This is the repository for the in-incubator microscope. It is capable to acquire Time-Lapse videos from living cells inside an incubator.
The current version of the Z-stage is inspired by the flexure-bearing Z-stage from Richard Bowman's openflexure microscope design. Their open-source project can be found here.
Each functional block has an Arduino acting as a BUS-communicator. Therefore it's possible to control (i.e. switch on/off) devices from the MASTER-device (i.e. Raspberry pi). We aim to build a low-cost solution for high throughput acquisitions, acting as a new tools for (not only) biologists.
It is a very early developer version. Please feel free to contribute to the documentation and device development.
Note: The pictures on this site do not show the latest version of the Z-stage. Follow the respective assembly guidelines.
- X/Y/Z/t Acquisitions possible
- Fluorescent imaging
- Quantitative Phase Imaging
- Modularized design
- Low-cost acquisition
- Based on off-the-shelf components
- Open-Source
The Bill of Materials is always the most up-to-date version!
| Name | Properties | Price | Link | # |
|---|---|---|---|---|
| Baseplate puzzle | - | 5€ | Base-plate | 5 |
| Module: Z-Stage | - | ?? € | Z-Stage | 1 |
| Module: Mirror 45° | - | 5€ | Mirror 45 | 1 |
| Module: Raspberry Camera | - | ??€ | RaspiCam | 1 |
| Module: LED array | - | ??€ | LED array | 1 |
To acquire the STL-files use the UC2-Configurator. The files themselves are in the RAW folder. The module can be built using injection-moulded (IM) or 3D-printed (3DP) cubes.
- Check out the RESOURCES for more information!
- 1× LED-Array, Neopixel, 8x8 🢂
- 32× 5mm Ball magnets 🢂
- 20× - 40× Screws DIN912 ISO 4762 M3×12 mm 🢂
- 1× M3×30 mm and M3 nut - non-magnetic
- 2× M2×12 mm and 2× M2 nut - non-magnetic
- 1× Raspberry Pi Camera 🢂
- 1× Objective Lens (e.g. 10×, NA 0.3) 🢂
- 1× Mirrors (e.g. 30×30 mm² Toymirrors) 🢂
- 2× ESP32 🢂
- 1× Stepper Motor and 1× Driver Board 🢂
- 9× Female-Female Jumper Wire, 0.14 mm² 🢂
- 2× Power cables for ESP32 - USB-microUSB 🢂
- Optional (for Fluomodule): 2× Star-LEDs blue (high power 1-3 Watt) 🢂
- Optional (for Fluomodule): 1x MOS-FET/Power PNP Transistor 🢂
- Optional (for Fluomodule): 4× Screws DIN912 ISO 4762 M3×18 mm 🢂
For assembly instructions of the respective modules refer to the links in Modules for this setup.
A simplified scheme can be found here.
A detailed alignment instructions are also found here.
🢂 Find more in the Electronics section
🢂 Find the software for this setup in our dedicated UC2-Software-GIT
This is a 30-minutes timelapse of some dog-cells made in a Workshop in UiO prepared by Xian H. and Kay S.
Do you want to show your own results? Do you have ideas for improvements? Let us know!