diff --git a/instrumentation/opentelemetry-instrumentation-aiohttp-server/pyproject.toml b/instrumentation/opentelemetry-instrumentation-aiohttp-server/pyproject.toml
index 566f9dbb35..3ea2340d89 100644
--- a/instrumentation/opentelemetry-instrumentation-aiohttp-server/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-aiohttp-server/pyproject.toml
@@ -35,11 +35,6 @@ dependencies = [
 instruments = [
     "aiohttp ~= 3.0",
 ]
-test = [
-  "opentelemetry-instrumentation-aiohttp-server[instruments]",
-  "pytest-asyncio",
-  "pytest-aiohttp",
-]
 
 [project.entry-points.opentelemetry_instrumentor]
 aiohttp-server = "opentelemetry.instrumentation.aiohttp_server:AioHttpServerInstrumentor"
diff --git a/instrumentation/opentelemetry-instrumentation-aiohttp-server/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-aiohttp-server/test-requirements.txt
new file mode 100644
index 0000000000..0e49aeb745
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-aiohttp-server/test-requirements.txt
@@ -0,0 +1,27 @@
+aiohttp==3.9.3
+aiosignal==1.3.1
+asgiref==3.7.2
+async-timeout==4.0.3
+attrs==23.2.0
+Deprecated==1.2.14
+frozenlist==1.4.1
+idna==3.6
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+multidict==6.0.5
+packaging==23.2
+pluggy==1.4.0
+py==1.11.0
+py-cpuinfo==9.0.0
+pytest==7.1.3
+pytest-aiohttp==1.0.5
+pytest-asyncio==0.23.5
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.10.0
+wrapt==1.16.0
+yarl==1.9.4
+zipp==3.17.0
+-e ../../../opentelemetry-instrumentation
+-e ../../../util/opentelemetry-util-http
+-e ../../../instrumentation/opentelemetry-instrumentation-aiohttp-server
diff --git a/tox.ini b/tox.ini
index 820ac93816..b0ed755ed8 100644
--- a/tox.ini
+++ b/tox.ini
@@ -391,7 +391,7 @@ commands_pre =
 
   grpc: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-grpc[test]
 
-  falcon-{1,2,3},flask-{213,220,300},django-{1,2,3,4},pyramid,tornado,starlette,fastapi,aiohttp,asgi,httpx-{18,21},requests,urllib,urllib3v-{1,2},wsgi: pip install {toxinidir}/util/opentelemetry-util-http
+  falcon-{1,2,3},flask-{213,220,300},django-{1,2,3,4},pyramid,tornado,starlette,fastapi,aiohttp-client,asgi,httpx-{18,21},requests,urllib,urllib3v-{1,2},wsgi: pip install {toxinidir}/util/opentelemetry-util-http
   wsgi,falcon-{1,2,3},flask-{213,220,300},django-{1,2,3,4},pyramid: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-wsgi[test]
   asgi,django-{3,4},starlette,fastapi: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-asgi[test]
 
@@ -462,7 +462,7 @@ commands_pre =
 
   aiohttp-client: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-client[test]
 
-  aiohttp-server: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-server[test]
+  aiohttp-server: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-server/test-requirements.txt
 
   aiopg: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-aiopg[test]
 
@@ -565,7 +565,7 @@ commands_pre =
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-psycopg[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-psycopg2[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-client[test]
-  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-server[test]
+  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-server
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-aiopg[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-sqlite3[test]
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pyramid[test]