diff --git a/.github/test-spec.yml b/.github/test-spec.yml
index c01ae703adf..007f4307aa9 100644
--- a/.github/test-spec.yml
+++ b/.github/test-spec.yml
@@ -218,3 +218,25 @@
       - "subsys/bluetooth/**/*"
       - "!subsys/bluetooth/mesh/**/*"
       - "!subsys/bluetooth/audio/**/*"
+
+"CI-audio-test":
+  - "boards/arm/nrf5340_audio_dk_nrf5340/**/*"
+  - "drivers/flash/**/*"
+  - "drivers/spi/**/*"
+  - "drivers/gpio/**/*"
+  - "drivers/i2c/**/*"
+  - "drivers/watchdog/**/*"
+  - "include/dfu/**/*"
+  - "include/mgmt/mcumgr/**/*"
+  - "samples/bluetooth/hci_rpmsg/**/*"
+  - "soc/arm/nordic_nrf/**/*"
+  - "subsys/bluetooth/audio/**/*"
+  - "subsys/bluetooth/host/**/*"
+  - "subsys/dfu/**/*"
+  - "subsys/fs/**/*"
+  - "subsys/mgmt/mcumgr/**/*"
+  - "subsys/sd/**/*"
+  - "subsys/storage/**/*"
+  - "subsys/task_wdt/**/*"
+  - "subsys/usb/**/*"
+  - "subsys/zbus/**/*"