diff --git a/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_common.dtsi b/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_common.dtsi
index 9aa338deaad..ae810c27966 100644
--- a/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_common.dtsi
+++ b/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_common.dtsi
@@ -3,8 +3,11 @@
 &pinctrl {
 	uart137_default_alt: uart137_default_alt {
 		group1 {
-			psels = <NRF_PSEL(UART_TX, 0, 6)>,
-				<NRF_PSEL(UART_RX, 0, 7)>;
+			psels = <NRF_PSEL(UART_TX, 0, 6)>;
+		};
+		group2 {
+			psels = <NRF_PSEL(UART_RX, 0, 7)>;
+			bias-pull-up;
 		};
 	};
 
@@ -15,6 +18,23 @@
 			low-power-enable;
 		};
 	};
+	uart120_default_alt: uart120_default_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 7, 7)>;
+		};
+		group2 {
+			psels = <NRF_PSEL(UART_RX, 7, 4)>;
+			bias-pull-up;
+		};
+	};
+
+	uart120_sleep_alt: uart120_sleep_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 7, 7)>,
+				<NRF_PSEL(UART_RX, 7, 4)>;
+			low-power-enable;
+		};
+	};
 };
 
 dut: &uart137 {
@@ -24,3 +44,10 @@ dut: &uart137 {
 	pinctrl-names = "default", "sleep";
 	current-speed = <115200>;
 };
+
+dut2: &uart120 {
+	pinctrl-0 = <&uart120_default_alt>;
+	pinctrl-1 = <&uart120_sleep_alt>;
+	pinctrl-names = "default", "sleep";
+	current-speed = <115200>;
+};
diff --git a/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index be5897fd051..79f5554a33b 100644
--- a/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/tests/drivers/uart/uart_async_api/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -5,3 +5,12 @@
 &dut {
 	memory-regions = <&cpuapp_dma_region>;
 };
+
+&dut2 {
+	status = "okay";
+	memory-regions = <&dma_fast_region>;
+};
+
+&dma_fast_region {
+	status = "okay";
+};
diff --git a/tests/drivers/uart/uart_async_api/src/test_uart_async.c b/tests/drivers/uart/uart_async_api/src/test_uart_async.c
index 139de516fd8..c087878eee8 100644
--- a/tests/drivers/uart/uart_async_api/src/test_uart_async.c
+++ b/tests/drivers/uart/uart_async_api/src/test_uart_async.c
@@ -31,12 +31,17 @@ struct dut_data {
 	const char *name;
 };
 
-static ZTEST_DMEM struct dut_data duts[] = {
+ZTEST_DMEM struct dut_data duts[] = {
 	{
 		.dev = DEVICE_DT_GET(UART_NODE),
 		.name = DT_NODE_FULL_NAME(UART_NODE),
 	},
-	/* More instances can be added here. */
+#if DT_NODE_EXISTS(DT_NODELABEL(dut2)) && DT_NODE_HAS_STATUS(DT_NODELABEL(dut2), okay)
+	{
+		.dev = DEVICE_DT_GET(DT_NODELABEL(dut2)),
+		.name = DT_NODE_FULL_NAME(DT_NODELABEL(dut2)),
+	},
+#endif
 };
 
 static ZTEST_BMEM const struct device *uart_dev;