diff --git a/CHANGELOG.md b/CHANGELOG.md
index d0e1190e69..c19839df49 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,11 +21,13 @@
 - Added stub test creation to `create_test_yml` ([#2476](https://github.com/nf-core/tools/pull/2476))
 - Replace ModulePatch by ComponentPatch ([#2482](https://github.com/nf-core/tools/pull/2482))
 - Fixed `nf-core modules lint` to work with new module structure for nf-test ([#2494](https://github.com/nf-core/tools/pull/2494))
+- Add option `--migrate-pytest` to create a module with nf-test taking into account an existing module ([#2549](https://github.com/nf-core/tools/pull/2549))
 
 ### Subworkflows
 
 - Added stub test creation to `create_test_yml` ([#2476](https://github.com/nf-core/tools/pull/2476))
 - Fixed `nf-core subworkflows lint` to work with new module structure for nf-test ([#2494](https://github.com/nf-core/tools/pull/2494))
+- Add option `--migrate-pytest` to create a subworkflow with nf-test taking into account an existing subworkflow ([#2549](https://github.com/nf-core/tools/pull/2549))
 
 ### General
 
diff --git a/nf_core/__main__.py b/nf_core/__main__.py
index b3728999c8..dfdd60f428 100644
--- a/nf_core/__main__.py
+++ b/nf_core/__main__.py
@@ -815,8 +815,20 @@ def modules_remove(ctx, dir, tool):
     default=False,
     help="Create a module from the template without TODOs or examples",
 )
+@click.option("--migrate-pytest", is_flag=True, default=False, help="Migrate a module with pytest tests to nf-test")
 def create_module(
-    ctx, tool, dir, author, label, meta, no_meta, force, conda_name, conda_package_version, empty_template
+    ctx,
+    tool,
+    dir,
+    author,
+    label,
+    meta,
+    no_meta,
+    force,
+    conda_name,
+    conda_package_version,
+    empty_template,
+    migrate_pytest,
 ):
     """
     Create a new DSL2 module from the nf-core template.
@@ -841,7 +853,7 @@ def create_module(
     # Run function
     try:
         module_create = ModuleCreate(
-            dir, tool, author, label, has_meta, force, conda_name, conda_package_version, empty_template
+            dir, tool, author, label, has_meta, force, conda_name, conda_package_version, empty_template, migrate_pytest
         )
         module_create.create()
     except UserWarning as e:
@@ -1033,7 +1045,8 @@ def bump_versions(ctx, tool, dir, all, show_all):
 @click.option("-d", "--dir", type=click.Path(exists=True), default=".", metavar="<directory>")
 @click.option("-a", "--author", type=str, metavar="<author>", help="Module author's GitHub username prefixed with '@'")
 @click.option("-f", "--force", is_flag=True, default=False, help="Overwrite any files if they already exist")
-def create_subworkflow(ctx, subworkflow, dir, author, force):
+@click.option("--migrate-pytest", is_flag=True, default=False, help="Migrate a module with pytest tests to nf-test")
+def create_subworkflow(ctx, subworkflow, dir, author, force, migrate_pytest):
     """
     Create a new subworkflow from the nf-core template.
 
@@ -1047,7 +1060,7 @@ def create_subworkflow(ctx, subworkflow, dir, author, force):
 
     # Run function
     try:
-        subworkflow_create = SubworkflowCreate(dir, subworkflow, author, force)
+        subworkflow_create = SubworkflowCreate(dir, subworkflow, author, force, migrate_pytest)
         subworkflow_create.create()
     except UserWarning as e:
         log.critical(e)
diff --git a/nf_core/components/create.py b/nf_core/components/create.py
index 54fade29a2..2105104922 100644
--- a/nf_core/components/create.py
+++ b/nf_core/components/create.py
@@ -9,6 +9,7 @@
 import logging
 import os
 import re
+import shutil
 import subprocess
 from pathlib import Path
 from typing import Dict, Optional
@@ -16,11 +17,13 @@
 import jinja2
 import questionary
 import rich
+import yaml
 from packaging.version import parse as parse_version
 
 import nf_core
 import nf_core.utils
 from nf_core.components.components_command import ComponentCommand
+from nf_core.lint_utils import run_prettier_on_file
 
 log = logging.getLogger(__name__)
 
@@ -38,6 +41,7 @@ def __init__(
         conda_name: Optional[str] = None,
         conda_version: Optional[str] = None,
         empty_template: bool = False,
+        migrate_pytest: bool = False,
     ):
         super().__init__(component_type, directory)
         self.directory = directory
@@ -58,6 +62,7 @@ def __init__(
         self.docker_container = None
         self.file_paths: Dict[str, str] = {}
         self.not_empty_template = not empty_template
+        self.migrate_pytest = migrate_pytest
 
     def create(self):
         """
@@ -136,20 +141,35 @@ def create(self):
         # Check existence of directories early for fast-fail
         self.file_paths = self._get_component_dirs()
 
-        if self.component_type == "modules":
-            # Try to find a bioconda package for 'component'
-            self._get_bioconda_tool()
+        if self.migrate_pytest:
+            # Rename the component directory to old
+            component_old_dir = self.component_dir + "_old"
+            component_parent_path = Path(self.directory, self.component_type, self.org)
+            component_old_path = component_parent_path / component_old_dir
+            component_path = component_parent_path / self.component_dir
 
-        # Prompt for GitHub username
-        self._get_username()
+            component_path.rename(component_old_path)
+        else:
+            if self.component_type == "modules":
+                # Try to find a bioconda package for 'component'
+                self._get_bioconda_tool()
 
-        if self.component_type == "modules":
-            self._get_module_structure_components()
+            # Prompt for GitHub username
+            self._get_username()
+
+            if self.component_type == "modules":
+                self._get_module_structure_components()
 
         # Create component template with jinja2
         self._render_template()
         log.info(f"Created component template: '{self.component_name}'")
 
+        if self.migrate_pytest:
+            self._copy_old_files(component_old_path)
+            log.info("Migrate pytest tests: Copied original module files to new module")
+            shutil.rmtree(component_old_path)
+            self._print_and_delete_pytest_files()
+
         new_files = list(self.file_paths.values())
         log.info("Created following files:\n  " + "\n  ".join(new_files))
 
@@ -348,7 +368,7 @@ def _get_component_dirs(self):
             component_dir = os.path.join(self.directory, self.component_type, self.org, self.component_dir)
 
             # Check if module/subworkflow directories exist already
-            if os.path.exists(component_dir) and not self.force_overwrite:
+            if os.path.exists(component_dir) and not self.force_overwrite and not self.migrate_pytest:
                 raise UserWarning(
                     f"{self.component_type[:-1]} directory exists: '{component_dir}'. Use '--force' to overwrite"
                 )
@@ -358,7 +378,7 @@ def _get_component_dirs(self):
                 parent_tool_main_nf = os.path.join(
                     self.directory, self.component_type, self.org, self.component, "main.nf"
                 )
-                if self.subtool and os.path.exists(parent_tool_main_nf):
+                if self.subtool and os.path.exists(parent_tool_main_nf) and not self.migrate_pytest:
                     raise UserWarning(
                         f"Module '{parent_tool_main_nf}' exists already, cannot make subtool '{self.component_name}'"
                     )
@@ -367,7 +387,7 @@ def _get_component_dirs(self):
                 tool_glob = glob.glob(
                     f"{os.path.join(self.directory, self.component_type, self.org, self.component)}/*/main.nf"
                 )
-                if not self.subtool and tool_glob:
+                if not self.subtool and tool_glob and not self.migrate_pytest:
                     raise UserWarning(
                         f"Module subtool '{tool_glob[0]}' exists already, cannot make tool '{self.component_name}'"
                     )
@@ -411,3 +431,61 @@ def _get_username(self):
                 f"[violet]GitHub Username:[/]{' (@author)' if author_default is None else ''}",
                 default=author_default,
             )
+
+    def _copy_old_files(self, component_old_path):
+        """Copy files from old module to new module"""
+        log.debug("Copying original main.nf file")
+        shutil.copyfile(component_old_path / "main.nf", self.file_paths[self.component_type + "/main.nf"])
+        log.debug("Copying original meta.yml file")
+        shutil.copyfile(component_old_path / "meta.yml", self.file_paths[self.component_type + "/meta.yml"])
+        if self.component_type == "modules":
+            log.debug("Copying original environment.yml file")
+            shutil.copyfile(
+                component_old_path / "environment.yml", self.file_paths[self.component_type + "/environment.yml"]
+            )
+        # Create a nextflow.config file if it contains information other than publishDir
+        pytest_dir = Path(self.directory, "tests", self.component_type, self.org, self.component_dir)
+        nextflow_config = pytest_dir / "nextflow.config"
+        if nextflow_config.is_file():
+            with open(nextflow_config, "r") as fh:
+                config_lines = ""
+                for line in fh:
+                    if "publishDir" not in line:
+                        config_lines += line
+            if len(config_lines) > 0:
+                log.debug("Copying nextflow.config file from pytest tests")
+                with open(
+                    Path(self.directory, self.component_type, self.org, self.component_dir, "tests", "nextflow.config"),
+                    "w+",
+                ) as ofh:
+                    ofh.write(config_lines)
+
+    def _print_and_delete_pytest_files(self):
+        """Prompt if pytest files should be deleted and printed to stdout"""
+        pytest_dir = Path(self.directory, "tests", self.component_type, self.org, self.component_dir)
+        if rich.prompt.Confirm.ask(
+            "[violet]Do you want to delete the pytest files?[/]\nPytest file 'main.nf' will be printed to standard output to allow migrating the tests manually to 'main.nf.test'.",
+            default=False,
+        ):
+            with open(pytest_dir / "main.nf", "r") as fh:
+                log.info(fh.read())
+            shutil.rmtree(pytest_dir)
+            log.info(
+                "[yellow]Please convert the pytest tests to nf-test in 'main.nf.test'.[/]\n"
+                "You can find more information about nf-test [link=https://nf-co.re/docs/contributing/modules#migrating-from-pytest-to-nf-test]at the nf-core web[/link]. "
+            )
+        else:
+            log.info(
+                "[yellow]Please migrate the pytest tests to nf-test in 'main.nf.test'.[/]\n"
+                "You can find more information about nf-test [link=https://nf-co.re/docs/contributing/modules#migrating-from-pytest-to-nf-test]at the nf-core web[/link].\n"
+                f"Once done, make sure to delete the module pytest files to avoid linting errors: {pytest_dir}"
+            )
+        # Delete tags from pytest_modules.yml
+        modules_yml = Path(self.directory, "tests", "config", "pytest_modules.yml")
+        with open(modules_yml, "r") as fh:
+            yml_file = yaml.safe_load(fh)
+        yml_key = self.component_dir if self.component_type == "modules" else f"subworkflows/{self.component_dir}"
+        del yml_file[yml_key]
+        with open(modules_yml, "w") as fh:
+            yaml.dump(yml_file, fh)
+        run_prettier_on_file(modules_yml)
diff --git a/nf_core/modules/create.py b/nf_core/modules/create.py
index b5368130ce..a5e0795a9f 100644
--- a/nf_core/modules/create.py
+++ b/nf_core/modules/create.py
@@ -17,6 +17,7 @@ def __init__(
         conda_name=None,
         conda_version=None,
         empty_template=False,
+        migrate_pytest=False,
     ):
         super().__init__(
             "modules",
@@ -29,4 +30,5 @@ def __init__(
             conda_name,
             conda_version,
             empty_template,
+            migrate_pytest,
         )
diff --git a/nf_core/subworkflows/create.py b/nf_core/subworkflows/create.py
index 963076455e..93e9f271be 100644
--- a/nf_core/subworkflows/create.py
+++ b/nf_core/subworkflows/create.py
@@ -12,6 +12,7 @@ def __init__(
         component="",
         author=None,
         force=False,
+        migrate_pytest=False,
     ):
         super().__init__(
             "subworkflows",
@@ -19,4 +20,5 @@ def __init__(
             component,
             author,
             force=force,
+            migrate_pytest=migrate_pytest,
         )
diff --git a/tests/modules/create.py b/tests/modules/create.py
index 7cfba484a1..74e5ec3896 100644
--- a/tests/modules/create.py
+++ b/tests/modules/create.py
@@ -1,11 +1,22 @@
+import filecmp
 import os
+import shutil
+from pathlib import Path
+from unittest import mock
 
 import pytest
 import requests_cache
 import responses
+import yaml
+from git.repo import Repo
 
 import nf_core.modules
-from tests.utils import mock_anaconda_api_calls, mock_biocontainers_api_calls
+from tests.utils import (
+    GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH,
+    GITLAB_URL,
+    mock_anaconda_api_calls,
+    mock_biocontainers_api_calls,
+)
 
 
 def test_modules_create_succeed(self):
@@ -65,3 +76,66 @@ def test_modules_create_nfcore_modules_subtool(self):
     assert os.path.exists(
         os.path.join(self.nfcore_modules, "modules", "nf-core", "star", "index", "tests", "main.nf.test")
     )
+
+
+@mock.patch("rich.prompt.Confirm.ask")
+def test_modules_migrate(self, mock_rich_ask):
+    """Create a module with the --migrate-pytest option to convert pytest to nf-test"""
+    pytest_dir = Path(self.nfcore_modules, "tests", "modules", "nf-core", "samtools", "sort")
+    module_dir = Path(self.nfcore_modules, "modules", "nf-core", "samtools", "sort")
+
+    # Clone modules repo with pytests
+    shutil.rmtree(self.nfcore_modules)
+    Repo.clone_from(GITLAB_URL, self.nfcore_modules, branch=GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH)
+    with open(module_dir / "main.nf", "r") as fh:
+        old_main_nf = fh.read()
+    with open(module_dir / "meta.yml", "r") as fh:
+        old_meta_yml = fh.read()
+
+    # Create a module with --migrate-pytest
+    mock_rich_ask.return_value = True
+    module_create = nf_core.modules.ModuleCreate(self.nfcore_modules, "samtools/sort", migrate_pytest=True)
+    module_create.create()
+
+    with open(module_dir / "main.nf", "r") as fh:
+        new_main_nf = fh.read()
+    with open(module_dir / "meta.yml", "r") as fh:
+        new_meta_yml = fh.read()
+    nextflow_config = module_dir / "tests" / "nextflow.config"
+
+    # Check that old files have been copied to the new module
+    assert old_main_nf == new_main_nf
+    assert old_meta_yml == new_meta_yml
+    assert nextflow_config.is_file()
+
+    # Check that pytest folder is deleted
+    assert not pytest_dir.is_dir()
+
+    # Check that pytest_modules.yml is updated
+    with open(Path(self.nfcore_modules, "tests", "config", "pytest_modules.yml")) as fh:
+        modules_yml = yaml.safe_load(fh)
+    assert "samtools/sort" not in modules_yml.keys()
+
+
+@mock.patch("rich.prompt.Confirm.ask")
+def test_modules_migrate_no_delete(self, mock_rich_ask):
+    """Create a module with the --migrate-pytest option to convert pytest to nf-test.
+    Test that pytest directory is not deleted."""
+    pytest_dir = Path(self.nfcore_modules, "tests", "modules", "nf-core", "samtools", "sort")
+
+    # Clone modules repo with pytests
+    shutil.rmtree(self.nfcore_modules)
+    Repo.clone_from(GITLAB_URL, self.nfcore_modules, branch=GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH)
+
+    # Create a module with --migrate-pytest
+    mock_rich_ask.return_value = False
+    module_create = nf_core.modules.ModuleCreate(self.nfcore_modules, "samtools/sort", migrate_pytest=True)
+    module_create.create()
+
+    # Check that pytest folder is not deleted
+    assert pytest_dir.is_dir()
+
+    # Check that pytest_modules.yml is updated
+    with open(Path(self.nfcore_modules, "tests", "config", "pytest_modules.yml")) as fh:
+        modules_yml = yaml.safe_load(fh)
+    assert "samtools/sort" not in modules_yml.keys()
diff --git a/tests/subworkflows/create.py b/tests/subworkflows/create.py
index 94c2a66331..fc628df34f 100644
--- a/tests/subworkflows/create.py
+++ b/tests/subworkflows/create.py
@@ -1,8 +1,15 @@
+import filecmp
 import os
+import shutil
+from pathlib import Path
+from unittest import mock
 
 import pytest
+import yaml
+from git.repo import Repo
 
 import nf_core.subworkflows
+from tests.utils import GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH, GITLAB_URL
 
 
 def test_subworkflows_create_succeed(self):
@@ -35,3 +42,70 @@ def test_subworkflows_create_nfcore_modules(self):
     assert os.path.exists(
         os.path.join(self.nfcore_modules, "subworkflows", "nf-core", "test_subworkflow", "tests", "main.nf.test")
     )
+
+
+@mock.patch("rich.prompt.Confirm.ask")
+def test_subworkflows_migrate(self, mock_rich_ask):
+    """Create a subworkflow with the --migrate-pytest option to convert pytest to nf-test"""
+    pytest_dir = Path(self.nfcore_modules, "tests", "subworkflows", "nf-core", "bam_stats_samtools")
+    subworkflow_dir = Path(self.nfcore_modules, "subworkflows", "nf-core", "bam_stats_samtools")
+
+    # Clone modules repo with pytests
+    shutil.rmtree(self.nfcore_modules)
+    Repo.clone_from(GITLAB_URL, self.nfcore_modules, branch=GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH)
+    with open(subworkflow_dir / "main.nf", "r") as fh:
+        old_main_nf = fh.read()
+    with open(subworkflow_dir / "meta.yml", "r") as fh:
+        old_meta_yml = fh.read()
+
+    # Create a subworkflow with --migrate-pytest
+    mock_rich_ask.return_value = True
+    subworkflow_create = nf_core.subworkflows.SubworkflowCreate(
+        self.nfcore_modules, "bam_stats_samtools", migrate_pytest=True
+    )
+    subworkflow_create.create()
+
+    with open(subworkflow_dir / "main.nf", "r") as fh:
+        new_main_nf = fh.read()
+    with open(subworkflow_dir / "meta.yml", "r") as fh:
+        new_meta_yml = fh.read()
+    nextflow_config = subworkflow_dir / "tests" / "nextflow.config"
+
+    # Check that old files have been copied to the new module
+    assert old_main_nf == new_main_nf
+    assert old_meta_yml == new_meta_yml
+    assert nextflow_config.is_file()
+
+    # Check that pytest folder is deleted
+    assert not pytest_dir.is_dir()
+
+    # Check that pytest_modules.yml is updated
+    with open(Path(self.nfcore_modules, "tests", "config", "pytest_modules.yml")) as fh:
+        modules_yml = yaml.safe_load(fh)
+    assert "subworkflows/bam_stats_samtools" not in modules_yml.keys()
+
+
+@mock.patch("rich.prompt.Confirm.ask")
+def test_subworkflows_migrate_no_delete(self, mock_rich_ask):
+    """Create a subworkflow with the --migrate-pytest option to convert pytest to nf-test.
+    Test that pytest directory is not deleted."""
+    pytest_dir = Path(self.nfcore_modules, "tests", "subworkflows", "nf-core", "bam_stats_samtools")
+
+    # Clone modules repo with pytests
+    shutil.rmtree(self.nfcore_modules)
+    Repo.clone_from(GITLAB_URL, self.nfcore_modules, branch=GITLAB_SUBWORKFLOWS_ORG_PATH_BRANCH)
+
+    # Create a module with --migrate-pytest
+    mock_rich_ask.return_value = False
+    module_create = nf_core.subworkflows.SubworkflowCreate(
+        self.nfcore_modules, "bam_stats_samtools", migrate_pytest=True
+    )
+    module_create.create()
+
+    # Check that pytest folder is not deleted
+    assert pytest_dir.is_dir()
+
+    # Check that pytest_modules.yml is updated
+    with open(Path(self.nfcore_modules, "tests", "config", "pytest_modules.yml")) as fh:
+        modules_yml = yaml.safe_load(fh)
+    assert "subworkflows/bam_stats_samtools" not in modules_yml.keys()
diff --git a/tests/test_modules.py b/tests/test_modules.py
index 21a28d5e46..e2e024a751 100644
--- a/tests/test_modules.py
+++ b/tests/test_modules.py
@@ -152,6 +152,8 @@ def test_modulesrepo_class(self):
         test_modules_create_nfcore_modules,
         test_modules_create_nfcore_modules_subtool,
         test_modules_create_succeed,
+        test_modules_migrate,
+        test_modules_migrate_no_delete,
     )
     from .modules.info import (  # type: ignore[misc]
         test_modules_info_in_modules_repo,
diff --git a/tests/test_subworkflows.py b/tests/test_subworkflows.py
index a4ab959fb4..aaf4080a7b 100644
--- a/tests/test_subworkflows.py
+++ b/tests/test_subworkflows.py
@@ -92,6 +92,8 @@ def tearDown(self):
         test_subworkflows_create_fail_exists,
         test_subworkflows_create_nfcore_modules,
         test_subworkflows_create_succeed,
+        test_subworkflows_migrate,
+        test_subworkflows_migrate_no_delete,
     )
     from .subworkflows.info import (  # type: ignore[misc]
         test_subworkflows_info_in_modules_repo,