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Some versions of samtools and bcftools are not compatible so the pileup file generated by samtools mpileup cannot be read by bcftools call. Also, it seems bcftools call accepts only a piped input from bcftools mpileup. So the pileup files generated by samtools mpileup cannot finally be used for variant calling! I know there might be some ways to figure it out without adding a new feature to the pipeline but I have two suggestions to make Sarek even better:
Replace samtools mpileup with bcftools mpileup.
Add bcftool call for actual variant calling and generate the final vcf file.
The text was updated successfully, but these errors were encountered:
FriederikeHanssen
changed the title
Using bcftools mpileup (instead of samtools mpileup) for generating the pileup files
Fix bcftools mpileup
Jun 14, 2023
Description of feature
Some versions of samtools and bcftools are not compatible so the pileup file generated by
samtools mpileup
cannot be read bybcftools call
. Also, it seemsbcftools call
accepts only a piped input frombcftools mpileup
. So the pileup files generated bysamtools mpileup
cannot finally be used for variant calling! I know there might be some ways to figure it out without adding a new feature to the pipeline but I have two suggestions to make Sarek even better:samtools mpileup
withbcftools mpileup
.The text was updated successfully, but these errors were encountered: