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Nanoseq Pipeline Fails at Bambu Process with java.lang.Boolean Error #279

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xeniaroda opened this issue Oct 7, 2024 · 0 comments
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@xeniaroda
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I am currently testing the nf-core/nanoseq pipeline with a transcriptomic dataset (RIP-Seq strategy). My objective is to run the RNA protocol followed by Bambu, and later perform differential expression analysis using DESeq2 and DEXseq. However, the pipeline gets stuck during the Bambu process with the following error:

Pipeline completed with errors
ERROR ~ Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:BAMBU (1)'

Caused by:
Not a valid path value type: java.lang.Boolean (false)

Image

  • Specifications:

Sample information:

Name: GSM6774547
Instrument: GridION
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: Other
Layout: SINGLE

  • Sample sheet (samplesheet.csv): is attached samplesheet.csv

  • Configuration file (out.config): is attached as a text file out.config.txt

  • Nextflow.log file (.nextflow.log) is attached as a text file nextflow.log.txt

  • Command:
    nextflow run nf-core/nanoseq -c /media/nfcore_nanoseq/out.config -profile docker

The pipeline runs as expected until the Bambu process, where it halts with the error. It seems the error is related to an incorrect path configuration or Boolean value in the configuration file or during the Bambu process. Any help resolving this would be appreciated!

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