diff --git a/CHANGELOG.md b/CHANGELOG.md
index 18ddc29e..63515e3f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,10 +9,16 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### `Changed`
 
+- [#664](https://github.com/nf-core/mag/pull/664) - Update GTDBTk to latest version, with updated column names, update GTDB to release 220 (by @dialvarezs)
+
 ### `Fixed`
 
 ### `Dependencies`
 
+| Tool   | Previous version | New version |
+| ------ | ---------------- | ----------- |
+| GTDBTk | 2.3.2            | 2.4.0       |
+
 ### `Deprecated`
 
 ## 3.0.3 [2024-08-27]
diff --git a/bin/summary_gtdbtk.py b/bin/summary_gtdbtk.py
index 7ae43a09..370ea4fa 100755
--- a/bin/summary_gtdbtk.py
+++ b/bin/summary_gtdbtk.py
@@ -76,11 +76,11 @@ def main(args=None):
     columns = [
         "user_genome",
         "classification",
-        "fastani_reference",
-        "fastani_reference_radius",
-        "fastani_taxonomy",
-        "fastani_ani",
-        "fastani_af",
+        "closest_genome_reference",
+        "closest_genome_reference_radius",
+        "closest_genome_taxonomy",
+        "closest_genome_ani",
+        "closest_genome_af",
         "closest_placement_reference",
         "closest_placement_radius",
         "closest_placement_taxonomy",
diff --git a/conf/test_full.config b/conf/test_full.config
index d7f3365e..9a01bc58 100644
--- a/conf/test_full.config
+++ b/conf/test_full.config
@@ -22,7 +22,7 @@ params {
     //centrifuge_db = "s3://ngi-igenomes/test-data/mag/p_compressed+h+v.tar.gz"
     kraken2_db    = "s3://ngi-igenomes/test-data/mag/minikraken_8GB_202003.tgz"
     cat_db        = "s3://ngi-igenomes/test-data/mag/CAT_prepare_20210107.tar.gz"
-    // gtdb_db       = "s3://ngi-igenomes/test-data/mag/gtdbtk_r214_data.tar.gz"
+    // gtdb_db       = "s3://ngi-igenomes/test-data/mag/gtdbtk_r214_data.tar.gz" ## This should be updated to release 220, once we get GTDB-Tk working again
     skip_gtdbtk   = true
 
     // TODO TEMPORARY: deactivate SPAdes due to incompatibility of container with fusion file system
diff --git a/modules.json b/modules.json
index 0cab4e4e..b17f7dc3 100644
--- a/modules.json
+++ b/modules.json
@@ -124,7 +124,7 @@
                     },
                     "gtdbtk/classifywf": {
                         "branch": "master",
-                        "git_sha": "0735b6d2b509cbb5cf71d15fda819cd7392722fe",
+                        "git_sha": "06c8865e36741e05ad32ef70ab3fac127486af48",
                         "installed_by": ["modules"]
                     },
                     "gunc/downloaddb": {
diff --git a/modules/nf-core/gtdbtk/classifywf/environment.yml b/modules/nf-core/gtdbtk/classifywf/environment.yml
index 8801269e..500531ea 100644
--- a/modules/nf-core/gtdbtk/classifywf/environment.yml
+++ b/modules/nf-core/gtdbtk/classifywf/environment.yml
@@ -1,7 +1,5 @@
-name: gtdbtk_classifywf
 channels:
   - conda-forge
   - bioconda
-  - defaults
 dependencies:
-  - bioconda::gtdbtk=2.3.2
+  - bioconda::gtdbtk=2.4.0
diff --git a/modules/nf-core/gtdbtk/classifywf/main.nf b/modules/nf-core/gtdbtk/classifywf/main.nf
index 14dd28a4..9fc7a32d 100644
--- a/modules/nf-core/gtdbtk/classifywf/main.nf
+++ b/modules/nf-core/gtdbtk/classifywf/main.nf
@@ -5,8 +5,8 @@ process GTDBTK_CLASSIFYWF {
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gtdbtk:2.3.2--pyhdfd78af_0' :
-        'biocontainers/gtdbtk:2.3.2--pyhdfd78af_0' }"
+        'https://depot.galaxyproject.org/singularity/gtdbtk:2.4.0--pyhdfd78af_1' :
+        'biocontainers/gtdbtk:2.4.0--pyhdfd78af_1' }"
 
     input:
     tuple val(meta), path("bins/*")
diff --git a/nextflow.config b/nextflow.config
index 85be4905..8338e9f3 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -87,7 +87,7 @@ params {
     cat_official_taxonomy                = false
     save_cat_db                          = false
     skip_gtdbtk                          = false
-    gtdb_db                              = "https://data.ace.uq.edu.au/public/gtdb/data/releases/release214/214.1/auxillary_files/gtdbtk_r214_data.tar.gz"
+    gtdb_db                              = "https://data.ace.uq.edu.au/public/gtdb/data/releases/release220/220.0/auxillary_files/gtdbtk_package/full_package/gtdbtk_r220_data.tar.gz"
     gtdb_mash                            = null
     gtdbtk_min_completeness              = 50.0
     gtdbtk_max_contamination             = 10.0
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 35e85825..219d6724 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -537,7 +537,7 @@
                 "gtdb_db": {
                     "type": "string",
                     "description": "Specify the location of a GTDBTK database. Can be either an uncompressed directory or a `.tar.gz` archive. If not specified will be downloaded for you when GTDBTK or binning QC is not skipped.",
-                    "default": "https://data.ace.uq.edu.au/public/gtdb/data/releases/release214/214.1/auxillary_files/gtdbtk_r214_data.tar.gz"
+                    "default": "https://data.ace.uq.edu.au/public/gtdb/data/releases/release220/220.0/auxillary_files/gtdbtk_package/full_package/gtdbtk_r220_data.tar.gz"
                 },
                 "gtdb_mash": {
                     "type": "string",