Spectral library search does not work for MALDI !

[Amon789]

Hi,
I use mzmine 4.4.0 for MALDI imaging and it works nice except that the library search generate no match.
I use the mzwizard tool as follows

Do you think some steps should be added to get spectral match with the library?

BR
Amon

[SteffenHeu]

Hi,

what kind of instrument are you using? how are the ms2 spectra generated for the dataset?

[Amon789]

Instrument: timsTOF flex MALDI and no MS2 spectra

[SteffenHeu]

Hi @Amon789,
what kind of spectral library do you want to search? They are usually contain MS2 spectra, so if you don't have any, i would also not expect any matches.
Since you have a timsTOF flex, maybe this can help you: https://www.nature.com/articles/s41467-023-43298-9