Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Integrate Bambu results for multiple samples #38

Open
koehlek99 opened this issue Jul 4, 2024 · 2 comments
Open

Integrate Bambu results for multiple samples #38

koehlek99 opened this issue Jul 4, 2024 · 2 comments

Comments

@koehlek99
Copy link

Hi, thanks a lot for developing this tool!

I ran the develop version using Bambu for transcript identification. I feel like I read somewhere that the multisample run for Bambu is not implemented yet, so I ran it separately on each sample.

Do you have a suggestion how to compare the novel transcripts and merge counts between samples? Do the Bambu IDs match or should I collapse them somehow?

Thanks a lot in advance!

Best,
Krissi

@ChangqingW
Copy link
Collaborator

Hmm, I did wrote not yet implemented for multi-sample. They do have a multi-sample mode. I'll try to hook it up with it.

@ChangqingW
Copy link
Collaborator

ChangqingW commented Jul 16, 2024

There is gffcompare to merge similar transcripts. If you do not merge similar ones, I guess your novel transcripts might be very sample-specific, e.g. when there is a genuine shared novel transcript across samples it might get called slightly differently in each sample.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants