diff --git a/packages/fluentui/CHANGELOG.md b/packages/fluentui/CHANGELOG.md
index 72d332cf7a4fdf..3bf2cfb7aab142 100644
--- a/packages/fluentui/CHANGELOG.md
+++ b/packages/fluentui/CHANGELOG.md
@@ -66,6 +66,7 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 - Fix `Loader` component to properly pass ref to root slots @chpalac ([#20814](https://github.com/microsoft/fluentui/pull/20814))
 - Fix `Label` component to properly pass ref to root slots @chpalac ([#20813](https://github.com/microsoft/fluentui/pull/20813))
 - Fix `MenuItemIcon` to allow icon `size` to be in effect @yuanboxue-amber ([#20803](https://github.com/microsoft/fluentui/pull/20803))
+- Fix `PopupContent` component to properly pass ref to root slots @chpalac ([#20866](https://github.com/microsoft/fluentui/pull/20866))
 - Fix `Pill` components to properly pass ref to root slots @chpalac ([#20838](https://github.com/microsoft/fluentui/pull/20838))
 - Fix `MenuButton` component to properly pass ref to root slots @chpalac ([#20837](https://github.com/microsoft/fluentui/pull/20837))
 - Fix `getPointerStyles` transform for RTL @chpalac ([#20915](https://github.com/microsoft/fluentui/pull/20915))
diff --git a/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.tsx b/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.tsx
index 1697fc3a8db220..9d36024a9e736f 100644
--- a/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.tsx
+++ b/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.tsx
@@ -1,4 +1,4 @@
-import { Ref } from '@fluentui/react-component-ref';
+import { handleRef } from '@fluentui/react-component-ref';
 import { callable } from '@fluentui/styles';
 import * as React from 'react';
 import * as PropTypes from 'prop-types';
@@ -16,6 +16,7 @@ export class AutoFocusZone extends React.Component<AutoFocusZoneProps> {
   static propTypes = {
     as: PropTypes.elementType,
     firstFocusableSelector: PropTypes.oneOfType([PropTypes.func, PropTypes.string]),
+    innerRef: PropTypes.any,
   };
 
   static handledProps = _.keys(AutoFocusZone.propTypes) as any;
@@ -29,9 +30,15 @@ export class AutoFocusZone extends React.Component<AutoFocusZoneProps> {
     const ElementType = getElementType(this.props);
 
     return (
-      <Ref innerRef={this.root}>
-        <ElementType {...unhandledProps}>{this.props.children}</ElementType>
-      </Ref>
+      <ElementType
+        ref={(element: HTMLElement) => {
+          handleRef(this.root, element);
+          handleRef(this.props.innerRef, element);
+        }}
+        {...unhandledProps}
+      >
+        {this.props.children}
+      </ElementType>
     );
   }
 
diff --git a/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.types.tsx b/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.types.tsx
index d19e57cc9559b2..2d8ef24bf035d9 100644
--- a/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.types.tsx
+++ b/packages/fluentui/react-bindings/src/FocusZone/AutoFocusZone.types.tsx
@@ -8,4 +8,6 @@ export interface AutoFocusZoneProps extends React.HTMLAttributes<HTMLDivElement>
    * Indicates the selector for first focusable item. By default, the first tabbable element will get focus.
    */
   firstFocusableSelector?: string | (() => string);
+
+  innerRef?: React.Ref<any>;
 }
diff --git a/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.tsx b/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.tsx
index e0fea4190f9f5c..9264ab38ebfc30 100644
--- a/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.tsx
+++ b/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.tsx
@@ -16,6 +16,7 @@ import {
   HIDDEN_FROM_ACC_TREE,
 } from './focusUtilities';
 import { FocusTrapZoneProps } from './FocusTrapZone.types';
+import { handleRef } from '@fluentui/react-component-ref';
 
 /** FocusTrapZone is used to trap the focus in any html element placed in body
  *  and hide other elements outside of Focus Trap Zone from accessibility tree.
@@ -56,6 +57,7 @@ export class FocusTrapZone extends React.Component<FocusTrapZoneProps, {}> {
     disableFirstFocus: PropTypes.bool,
     focusPreviouslyFocusedInnerElement: PropTypes.bool,
     focusTriggerOnOutsideClick: PropTypes.bool,
+    innerRef: PropTypes.any,
   };
 
   static defaultProps: FocusTrapZoneProps = {
@@ -140,7 +142,10 @@ export class FocusTrapZone extends React.Component<FocusTrapZoneProps, {}> {
         <ElementType
           {...unhandledProps}
           className={className}
-          ref={this.createRef}
+          ref={(element: HTMLElement) => {
+            this.createRef(element);
+            handleRef(this.props.innerRef, element);
+          }}
           aria-labelledby={ariaLabelledBy}
           onKeyDown={this._onKeyboardHandler}
           onFocusCapture={this._onFocusCapture}
diff --git a/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.types.tsx b/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.types.tsx
index 5b2b5ccfa9bea6..6aa07e18094306 100644
--- a/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.types.tsx
+++ b/packages/fluentui/react-bindings/src/FocusZone/FocusTrapZone.types.tsx
@@ -68,4 +68,6 @@ export interface FocusTrapZoneProps extends React.HTMLAttributes<HTMLDivElement>
    * If it has never had a focused descendant before, behavior falls back to the first focused descendant.
    */
   focusPreviouslyFocusedInnerElement?: boolean;
+
+  innerRef?: any;
 }
diff --git a/packages/fluentui/react-northstar/src/components/Popup/PopupContent.tsx b/packages/fluentui/react-northstar/src/components/Popup/PopupContent.tsx
index b5699e922dba29..20c436999530a4 100644
--- a/packages/fluentui/react-northstar/src/components/Popup/PopupContent.tsx
+++ b/packages/fluentui/react-northstar/src/components/Popup/PopupContent.tsx
@@ -1,6 +1,5 @@
 import { Accessibility } from '@fluentui/accessibility';
 import {
-  ComponentWithAs,
   AutoFocusZone,
   AutoFocusZoneProps,
   FocusTrapZone,
@@ -11,6 +10,7 @@ import {
   useStyles,
   useTelemetry,
   useUnhandledProps,
+  ForwardRefWithAs,
 } from '@fluentui/react-bindings';
 import * as customPropTypes from '@fluentui/react-proptypes';
 import * as PopperJs from '@popperjs/core';
@@ -90,8 +90,7 @@ export const popupContentSlotClassNames: PopupContentSlotClassNames = {
 /**
  * A PopupContent displays the content of a Popup component.
  */
-export const PopupContent: ComponentWithAs<'div', PopupContentProps> &
-  FluentComponentStaticProps<PopupContentProps> = props => {
+export const PopupContent = (React.forwardRef<HTMLDivElement, PopupContentProps>((props, ref) => {
   const context = useFluentContext();
   const { setStart, setEnd } = useTelemetry(PopupContent.displayName, context.telemetry);
   setStart();
@@ -162,22 +161,35 @@ export const PopupContent: ComponentWithAs<'div', PopupContentProps> &
       ...((_.keys(trapFocus).length && trapFocus) as FocusTrapZoneProps),
       as: ElementType,
     };
-    element = <FocusTrapZone {...focusTrapZoneProps}>{popupContent}</FocusTrapZone>;
+    element = (
+      <FocusTrapZone innerRef={ref} {...focusTrapZoneProps}>
+        {popupContent}
+      </FocusTrapZone>
+    );
   } else if (autoFocus) {
     const autoFocusZoneProps = {
       ...popupContentProps,
       ...((_.keys(autoFocus).length && autoFocus) as AutoFocusZoneProps),
       as: ElementType,
     };
-    element = <AutoFocusZone {...autoFocusZoneProps}>{popupContent}</AutoFocusZone>;
+    element = (
+      <AutoFocusZone innerRef={ref} {...autoFocusZoneProps}>
+        {popupContent}
+      </AutoFocusZone>
+    );
   } else {
-    element = <ElementType {...popupContentProps}>{popupContent}</ElementType>;
+    element = (
+      <ElementType ref={ref} {...popupContentProps}>
+        {popupContent}
+      </ElementType>
+    );
   }
 
   setEnd();
 
   return element;
-};
+}) as unknown) as ForwardRefWithAs<'div', HTMLDivElement, PopupContentProps> &
+  FluentComponentStaticProps<PopupContentProps>;
 
 PopupContent.displayName = 'PopupContent';