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feature barcode length limit cannot exceed 21 #383

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yuzhouevelyntong opened this issue Jun 2, 2023 · 0 comments
Open

feature barcode length limit cannot exceed 21 #383

yuzhouevelyntong opened this issue Jun 2, 2023 · 0 comments

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@yuzhouevelyntong
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Hey,

I'm running a custom Perturb-seq library thru cumulus. When it calls "run_generate_count_matrix_ADTs", it shows the error message "Barcode AGTCAAGACCCTCGGGCTCTGTGG exceeds the length limit 21!". Is this a set limit or can we customize it anywhere?

Also, I wonder why we cannot input the feature_barcode.csv file like what 10x website documented here
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@yuzhouevelyntong