diff --git a/unit-tests/live/options/test-timestamp-domain.py b/unit-tests/live/options/test-timestamp-domain.py
new file mode 100644
index 0000000000..bd58bd813d
--- /dev/null
+++ b/unit-tests/live/options/test-timestamp-domain.py
@@ -0,0 +1,83 @@
+# License: Apache 2.0. See LICENSE file in root directory.
+# Copyright(c) 2022 Intel Corporation. All Rights Reserved.
+
+# test:device D400*
+
+import time
+import pyrealsense2 as rs
+
+from rspy import test
+
+
+def close_resources(sensor):
+    """
+    Stop and Close sensor.
+    :sensor: sensor of device
+    """
+    if len(sensor.get_active_streams()) > 0:
+        sensor.stop()
+        sensor.close()
+
+
+def set_and_verify_timestamp_domain(sensor, global_time_enabled: bool):
+    """
+    Perform sensor (depth or color) test according given global time
+    :sensor: depth or color sensor in device
+    :global_time_enabled bool: True - timestamp is enabled otherwise false
+    """
+    global frame_queue
+
+    sensor.set_option(rs.option.global_time_enabled, global_time_enabled)
+    time.sleep(0.3)  # Waiting for new frame from device. Need in case low FPS.
+    frame = frame_queue.wait_for_frame()
+
+    if not frame:
+        test.fail()
+
+    expected_ts_domain = rs.timestamp_domain.global_time if global_time_enabled else \
+        rs.timestamp_domain.hardware_clock
+
+    test.check_equal(sensor.get_option(rs.option.global_time_enabled), global_time_enabled)
+    test.check_equal(frame.get_frame_timestamp_domain(), expected_ts_domain)
+
+
+frame_queue = rs.frame_queue(capacity=1, keep_frames=False)
+device = test.find_first_device_or_exit()
+
+# Depth sensor test
+depth_sensor = device.first_depth_sensor()
+depth_profile = next(p for p in depth_sensor.profiles if p.stream_type() == rs.stream.depth)
+depth_sensor.open(depth_profile)
+depth_sensor.start(frame_queue)
+
+# Test #1
+test.start('Check setting global time domain: depth sensor - timestamp domain is OFF')
+set_and_verify_timestamp_domain(depth_sensor, False)
+test.finish()
+
+# Test #2
+test.start('Check setting global time domain: depth sensor - timestamp domain is ON')
+set_and_verify_timestamp_domain(depth_sensor, True)
+test.finish()
+
+close_resources(depth_sensor)
+
+# Color sensor test
+color_sensor = device.first_color_sensor()
+color_profile = next(p for p in color_sensor.profiles if p.stream_type() == rs.stream.color)
+color_sensor.open(color_profile)
+color_sensor.start(frame_queue)
+
+# Test #3
+test.start('Check setting global time domain: color sensor - timestamp domain is OFF')
+set_and_verify_timestamp_domain(color_sensor, False)
+test.finish()
+
+# Test #4
+test.start('Check setting global time domain: color sensor - timestamp domain is ON')
+set_and_verify_timestamp_domain(color_sensor, True)
+test.finish()
+
+close_resources(color_sensor)
+
+test.print_results_and_exit()