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Hi,
I generated 3 matricss (exon, intron & spanning) using the -V option in dropEst from 10X BAM file. For velocity analysis, I wanted to use the matrices directly to generate an AnnData object & pass it to scVelo following the steps here. theislab/scvelo#78 (comment)
However, I could not generate the matrices due to the different size of the matrices.
As you can see, all my matrices have the same number of cells but different number of genes. I was wondering how can I generate matrices of similar size for all 3 of them so that I can use them for velocity analysis.
Thank you.
The text was updated successfully, but these errors were encountered:
benjytan88
changed the title
Matrices for velocyto
Matrices for velocity analysis
Jul 6, 2021
Hi,
I generated 3 matricss (exon, intron & spanning) using the -V option in dropEst from 10X BAM file. For velocity analysis, I wanted to use the matrices directly to generate an AnnData object & pass it to scVelo following the steps here.
theislab/scvelo#78 (comment)
However, I could not generate the matrices due to the different size of the matrices.
> dim(s19.dropest$exon)
[1] 14763 10418
> dim(s19.dropest$intron)
[1] 14362 10418
> dim(s19.dropest$spanning)
[1] 11804 10418
As you can see, all my matrices have the same number of cells but different number of genes. I was wondering how can I generate matrices of similar size for all 3 of them so that I can use them for velocity analysis.
Thank you.
The text was updated successfully, but these errors were encountered: