scvelo() ImportError: /lib64/libstdc++.so.6: version `GLIBCXX_3.4.21' not found (required by /home/shijian/.cache/basilisk/1.2.1/velociraptor-1.0.0/env/lib/python3.7/site-packages/pandas/_libs/window/aggregations.cpython-37m-x86_64-linux-gnu.so)

[shijianasdf]

I run scvelo() following the demo:

> velo.out <- scvelo(sce, subset.row=top.hvgs, assay.X="spliced")

and i get the following error:

Error in py_module_import(module, convert = convert) : .linux/moby/250bf1aceecb7cd934586ba31bbbf95535a3daf1cdac6f972043a22c1b875333 -address /run/containerd/c
ImportError: /lib64/libstdc++.so.6: version `GLIBCXX_3.4.21' not found (required by /home/shijian/.cache/basilisk/1.2.1/velociraptor-1.0.0/env/lib/python3.7/site-packages/pandas/_libs/window/aggregations.cpython-37m-x86_64-linux-gnu.so)

Detailed traceback: 
     File "/home/software/R-4.0.3/lib64/R/library/reticulate/python/rpytools/loader.py", line 44, in _import_hook
          level=level
     File "/home/shijian/.cache/basilisk/1.2.1/velociraptor-1.0.0/env/lib/python3.7/site-packages/anndata/__init__.py", line 1, in 
        <module>from ._core.anndata import AnnData, ImplicitModificationWarning
     File "/home/software/R-4.0.3/lib64/R/library/reticulate/python/rpytools/loader.py", line 44, in _import_hook level=level
     File "/home/shijian/.cache/basilisk/1.2.1/velociraptor-1.0.0/env/lib/python3.7/site-packages/anndata/_core/anndata.py", line 20,  in <module> import pandas as pd
     File "/home/software/R-4.0.3/lib64/R/library/reticulate/python/rpytools/loader.py", line 44, in _import_

[LTLA]

Hm. Nothing comes to mind. I'd suggest upgrading to BioC 3.13 in any case, basilisk is on 1.4.0 and you've got 1.2.1.

[YOU-k]

Same error with me, after update basilisk to 1.4.0

[YOU-k]

Error in py_module_import(module, convert = convert) : 
  ImportError: /lib64/libz.so.1: version `ZLIB_1.2.9' not found (required by /stornext/Home/data/allstaff/y/you.y/.cache/R/basilisk/1.4.0/velociraptor/1.2.0/env/lib/python3.7/site-packages/matplotlib/../../.././libpng16.so.16)

[LTLA]

I have absolutely no idea. Dunno why it's fiddling with a system library, this should not happen from inside a conda environment.

Best guess is that the environment activation failed such that the LDPATH was not set properly, but then you should have seen a warning message along the lines of "environment activation failed" or something like that.