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make_movie.m
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clear all;
generate_movie = 1;
% filename = 'Z:\pClamp\Station BN Data\09519009_Filt.abf';
filename = 'C:\Users\BLGyarfas\Documents\MATLAB\datafiles\09519009_Filt.abf';
video_output_name = 'FastVid3Step_With_Zoom2_Raw.avi';
animation_data = [];
start_time = 3.5;
end_time = 3.3;
[data, sampleInt] = import_abf(filename, 32);
animation_data = [animation_data; data(round(start_time/(sampleInt*1e-6)):end,:)];
[data, sampleInt] = import_abf(filename, 33);
animation_data = [animation_data; data(1:round(end_time/(sampleInt*1e-6)),:)];
time = 0:sampleInt*1e-6:(sampleInt*1e-6*(length(animation_data)-1));
h1 = figure('Position',[200 50 720 480]);
set(h1, 'DoubleBuffer', 'on', 'Color', [1 1 1]);
if generate_movie == 1
mov = avifile(video_output_name,'Compression', 'none', 'fps', 30, 'quality', 100);
end
%trying for 30 fps so plot every 33.3 ms
frame_per_sec = 30;
points_to_plot = round((1/30)/(sampleInt*1e-6));
seconds_to_plot = time(end);
frame_counter = 1;
with_voltage = 1;
if with_voltage == 0
%Without Voltage
for i=1:points_to_plot:round(seconds_to_plot/(sampleInt*1e-6))
h = plot(time(1:i), animation_data(1:i, 1));
axis([0 5 -20 30]);
xlabel('time (s)')
ylabel('pA')
tempInset = get(gca, 'TightInset');
tempPos = get(gca, 'Position');
tempOuter = get(gca, 'OuterPosition');
set(gca, 'Position', [tempInset(1) tempInset(2) 1-tempInset(3)-tempInset(1) 1-tempInset(4)-tempInset(2)])
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
end
end
else
%with voltage
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%FIRST PART - Real Time
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
acurrent = subplot(2,1,1);
axis([0 5 -20 65]);
ylabel('pA')
set(acurrent, 'XTickLabel', '', 'NextPlot', 'replacechildren');
set(acurrent, 'XLimMode', 'manual', 'YLimMode', 'manual');
tempInsetCurrent = get(acurrent, 'TightInset');
avoltage = subplot(2,1,2);
axis([0 5 -50 200]);
xlabel('time (s)')
ylabel('mV');
tempInsetVoltage = get(avoltage, 'TightInset');
set(avoltage, 'XLimMode', 'manual', 'YLimMode', 'manual', 'NextPlot', 'replacechildren');
shared_width = 1-(max([tempInsetCurrent(1) tempInsetVoltage(1)]) ...
+ max([tempInsetCurrent(3) tempInsetVoltage(3) ]));
shared_left = max([tempInsetCurrent(1) tempInsetVoltage(1)]);
set(acurrent,'Position', [shared_left 0.3+tempInsetCurrent(2) shared_width 0.7-(tempInsetCurrent(4)+tempInsetCurrent(2))])
set(avoltage,'Position', [shared_left tempInsetVoltage(2) shared_width 0.3-(tempInsetVoltage(4)+tempInsetVoltage(2))])
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '1x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
for i=1:points_to_plot:round(seconds_to_plot/(sampleInt*1e-6))
delete(hplotc)
delete(htext)
delete(hplotv)
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '1x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.001)
end
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%% HIGHLIGHT ZOOM AREA - FIRST ZOOM
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
hold on;
%Highlight Zoom, blink the line for 4 seconds
x = linspace(0,5*pi, 150); % gives 120 values
y = cos(x)/2 + 0.5;
axes(acurrent)
linehandle1 = line([2.75 2.75],[-20 65], [0 0], 'LineStyle', '--', 'Color', [y(1) y(1) y(1)], 'LineWidth', 2);
linehandle2 = line([4.25 4.25],[-20 65], [0 0], 'LineStyle', '--', 'Color', [y(1) y(1) y(1)], 'LineWidth', 2);
for i=2:length(y)
set(linehandle1, 'Color', [y(i) y(i) y(i)]);
set(linehandle2, 'Color', [y(i) y(i) y(i)]);
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%ZOOM IN - FIRST PART, CHANGE THE TIME TIME INDICIATOR FOR X1 TO X10
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
start_cur_axis = axis(acurrent);
start_volt_axis = axis(avoltage);
end_cur_axis = [2.75 4.25 -10 20];
end_volt_axis = [2.75 4.25 -50 100];
%Take 4 seconds to zoom in
%CALCULATE ZOOM
for i=1:4
trans_cur_axis(:,i) = start_cur_axis(i)-((tanh(linspace(-3,3,150)).*(1/2) + 0.5).*(start_cur_axis(i)-end_cur_axis(i)));
trans_volt_axis(:,i) = start_volt_axis(i)-((tanh(linspace(-3,3,150)).*(1/2) + 0.5).*(start_volt_axis(i)-end_volt_axis(i)));
end
trans_cur_axis = [start_cur_axis; trans_cur_axis; end_cur_axis];
trans_volt_axis = [start_volt_axis; trans_volt_axis; end_volt_axis];
%CACLUATE TEXT CHANGE
textchange = zeros(152,3);
% textchange(:,1) = cos(linspace(0,2*pi,152))./2+.5;
textchange(:,1) = ones(152,1);
textchange(:,2) = cos(linspace(pi,3*pi,152))./2+.5;
textchange(:,3) = cos(linspace(pi,3*pi,152))./2+.5;
for i=1:length(trans_cur_axis)
axis(acurrent, trans_cur_axis(i,:));
axis(avoltage, trans_volt_axis(i,:));
set(htext, 'Color', textchange(i,:));
if i > length(trans_cur_axis)/2
set(htext, 'String', 'x10')
end
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
delete(linehandle1)
delete(linehandle2)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%FADE OUT OLD TRACE
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
plotchange = zeros(150,3);
% textchange(:,1) = cos(linspace(0,2*pi,152))./2+.5;
plotchange(:,1) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,2) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,3) = cos(linspace(pi,2*pi,150))./2+.5;
for i=1:length(plotchange)
set(hplotc, 'Color', plotchange(i,:))
set(hplotv, 'Color', plotchange(i,:))
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
delete(hplotc)
delete(hplotv)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%REPLAY AT 10X
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
slow_down_factor = 10;
points_to_plot = round((1/30)/(sampleInt*1e-6*slow_down_factor));
start_time = 2.75;
end_time = 4.25;
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '10x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
for i=round(start_time/(sampleInt*1e-6)):points_to_plot:round(end_time/(sampleInt*1e-6))
delete(hplotc)
delete(htext)
delete(hplotv)
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '10x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.001)
end
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%ZOOM HIGHLIGHT AGAIN - 2ND TIME
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
x = linspace(0,5*pi, 150); % gives 120 values
y = cos(x)/2 + 0.5;
axes(acurrent)
linehandle1 = line([3.92 3.92],[-20 65], [0 0], 'LineStyle', '--', 'Color', [y(1) y(1) y(1)], 'LineWidth', 2);
linehandle2 = line([4.05 4.05],[-20 65], [0 0], 'LineStyle', '--', 'Color', [y(1) y(1) y(1)], 'LineWidth', 2);
for i=2:length(y)
set(linehandle1, 'Color', [y(i) y(i) y(i)]);
set(linehandle2, 'Color', [y(i) y(i) y(i)]);
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%% ZOOM IN - 2ND TIME
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
start_cur_axis = axis(acurrent);
start_volt_axis = axis(avoltage);
end_cur_axis = [3.92 4.05 -2.5 17.5];
end_volt_axis = [3.92 4.05 -50 100];
%Take 4 seconds to zoom in
%awesome zoom
trans_cur_axis = [];
trans_volt_axis = [];
for i=1:4
trans_cur_axis(:,i) = start_cur_axis(i)-((tanh(linspace(-3,3,150)).*(1/2) + 0.5).*(start_cur_axis(i)-end_cur_axis(i)));
trans_volt_axis(:,i) = start_volt_axis(i)-((tanh(linspace(-3,3,150)).*(1/2) + 0.5).*(start_volt_axis(i)-end_volt_axis(i)));
end
trans_cur_axis = [start_cur_axis; trans_cur_axis; end_cur_axis];
trans_volt_axis = [start_volt_axis; trans_volt_axis; end_volt_axis];
%text change from x10 to x30
textchange = zeros(152,3);
% textchange(:,1) = cos(linspace(0,2*pi,152))./2+.5;
textchange(:,1) = ones(152,1);
textchange(:,2) = cos(linspace(pi,3*pi,152))./2+.5;
textchange(:,3) = cos(linspace(pi,3*pi,152))./2+.5;
for i=1:length(trans_cur_axis)
axis(acurrent, trans_cur_axis(i,:));
axis(avoltage, trans_volt_axis(i,:));
set(htext, 'Color', textchange(i,:));
if i > length(trans_cur_axis)/2
set(htext, 'String', 'x100')
end
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
delete(linehandle1)
delete(linehandle2)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%FADE OUT OLD TRACE - 2ND TIME
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
plotchange = zeros(150,3);
% textchange(:,1) = cos(linspace(0,2*pi,152))./2+.5;
plotchange(:,1) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,2) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,3) = cos(linspace(pi,2*pi,150))./2+.5;
for i=1:length(plotchange)
set(hplotc, 'Color', plotchange(i,:))
set(hplotv, 'Color', plotchange(i,:))
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
delete(hplotc)
delete(hplotv)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%REPLAY AT 100X - 3RD REPLAY
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
slow_down_factor = 100;
points_to_plot = round((1/30)/(sampleInt*1e-6*slow_down_factor));
start_time = 3.92;
end_time = 4.05;
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '100x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
for i=round(start_time/(sampleInt*1e-6)):points_to_plot:round(end_time/(sampleInt*1e-6))
delete(hplotc)
delete(htext)
delete(hplotv)
axes(acurrent)
hplotc = plot(acurrent, time(1:i), animation_data(1:i, 1), '-k');
% text('Units', 'normalized', 'Position', [0.9 0.9 0], 'String', '1x','FontSize',24, 'Color', [1 0 0])
htext = text('Units', 'pixels', 'Position', [600 300 0], 'String', '100x','FontSize',24, 'Color', [1 0 0]);
axes(avoltage)
hplotv = plot(avoltage, time(1:i), animation_data(1:i, 2), '-k');
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.001)
end
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%FADE OUT OLD TRACE - 3RD TIME
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
plotchange = zeros(150,3);
% textchange(:,1) = cos(linspace(0,2*pi,152))./2+.5;
plotchange(:,1) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,2) = cos(linspace(pi,2*pi,150))./2+.5;
plotchange(:,3) = cos(linspace(pi,2*pi,150))./2+.5;
for i=1:length(plotchange)
set(hplotc, 'Color', plotchange(i,:))
set(hplotv, 'Color', plotchange(i,:))
if generate_movie == 1
F = getframe(gcf);
mov = addframe(mov, F);
else
pause(0.01)
end
end
delete(hplotc)
delete(hplotv)
end
if generate_movie == 1
mov = close(mov);
end