diff --git a/jwt/api_jwt.py b/jwt/api_jwt.py
index 48d739ad..9d035598 100644
--- a/jwt/api_jwt.py
+++ b/jwt/api_jwt.py
@@ -5,7 +5,7 @@
 from calendar import timegm
 from collections.abc import Iterable
 from datetime import datetime, timedelta, timezone
-from typing import TYPE_CHECKING, Any
+from typing import TYPE_CHECKING, Any, List
 
 from . import api_jws
 from .exceptions import (
@@ -110,7 +110,7 @@ def decode_complete(
         # passthrough arguments to _validate_claims
         # consider putting in options
         audience: str | Iterable[str] | None = None,
-        issuer: str | None = None,
+        issuer: str | List[str] | None = None,
         leeway: float | timedelta = 0,
         # kwargs
         **kwargs: Any,
@@ -195,7 +195,7 @@ def decode(
         # passthrough arguments to _validate_claims
         # consider putting in options
         audience: str | Iterable[str] | None = None,
-        issuer: str | None = None,
+        issuer: str | List[str] | None = None,
         leeway: float | timedelta = 0,
         # kwargs
         **kwargs: Any,
@@ -362,8 +362,12 @@ def _validate_iss(self, payload: dict[str, Any], issuer: Any) -> None:
         if "iss" not in payload:
             raise MissingRequiredClaimError("iss")
 
-        if payload["iss"] != issuer:
-            raise InvalidIssuerError("Invalid issuer")
+        if isinstance(issuer, list):
+            if payload["iss"] not in issuer:
+                raise InvalidIssuerError("Invalid issuer")
+        else:
+            if payload["iss"] != issuer:
+                raise InvalidIssuerError("Invalid issuer")
 
 
 _jwt_global_obj = PyJWT()
diff --git a/tests/test_api_jwt.py b/tests/test_api_jwt.py
index b51170b3..509f788f 100644
--- a/tests/test_api_jwt.py
+++ b/tests/test_api_jwt.py
@@ -486,6 +486,12 @@ def test_check_issuer_when_valid(self, jwt):
         token = jwt.encode(payload, "secret")
         jwt.decode(token, "secret", issuer=issuer, algorithms=["HS256"])
 
+    def test_check_issuer_list_when_valid(self, jwt):
+        issuer = ["urn:foo", "urn:bar"]
+        payload = {"some": "payload", "iss": "urn:foo"}
+        token = jwt.encode(payload, "secret")
+        jwt.decode(token, "secret", issuer=issuer, algorithms=["HS256"])
+
     def test_raise_exception_invalid_issuer(self, jwt):
         issuer = "urn:wrong"
 
@@ -496,6 +502,16 @@ def test_raise_exception_invalid_issuer(self, jwt):
         with pytest.raises(InvalidIssuerError):
             jwt.decode(token, "secret", issuer=issuer, algorithms=["HS256"])
 
+    def test_raise_exception_invalid_issuer_list(self, jwt):
+        issuer = ["urn:wrong", "urn:bar", "urn:baz"]
+
+        payload = {"some": "payload", "iss": "urn:foo"}
+
+        token = jwt.encode(payload, "secret")
+
+        with pytest.raises(InvalidIssuerError):
+            jwt.decode(token, "secret", issuer=issuer, algorithms=["HS256"])
+
     def test_skip_check_audience(self, jwt):
         payload = {"some": "payload", "aud": "urn:me"}
         token = jwt.encode(payload, "secret")