the tomogram is flipped in Z direction using the converted STAR file from WARP XML files

[werhoog]

Hi,
Thanks for the reliontomotools.
I meet with some problems, and can you help me?

I converted the XML files from WARP into the RELION4 STAR files.
commmand: warptomo2relion -i '../WARP/tilt/TS_005.xml' -s '../WARP/tilt/imod/005/TS_005.mrc' -d 1500 -o test_warp_relion --width 4092 --height 5760
After that, the tomograms were generated by the command "relion_tomo_reconstruct_tomogram" for manual inspection.

However, I found that the generated tomograms are Z-flipped compared with the tomograms generated by WARP.
Why are tomograms different generated by RELION4 and WARP? Any idea?

By the way, firstly, I did stack alignment in IMOD, and then import .xf files from imod into the WARP.
When the tilt series were imported from IMOD, the parameter "Don't invert angles" was not ticked in WARP.

Thanks!