README.md

EP-KP-transcriptomics

Additional Data for:

Raw data:

All data pertaining to this study are accessible at the European Nucleotide Archive (ENA) under the study accession PRJEB64484.

The King penguin transcriptome can be found under the ERS16107787 (HCAX01000001-HCAX01080605)

The Emperor penguin transcriptome can be found under ERS16107788 (HCAY01000001-HCAY01106060)

Clean reads from the 3’-QuantSeq mRNA data for each tissue and each species can also be found on the ENA (Study: PRJEB64484) under the sample accessions:

ERS16201405 - ERS16201453 (Emperor penguin)

ERS16201454 - ERS16201503 (King penguin)

Transcriptome annotations

Contains the Trinotate .tsv file for the annotation of both the de novo transcriptomes of the Emperor penguin and the King penguin

Long non-coding RNA datasets

King penguin

lncRNA_king_penguin.fa

Emperor penguin:

lncRNA_emperor_penguin.fa

Significant tissue-enhanced or tissue-inhibited transcripts

Data for both species are in the directory: tissue-enhanced-inhibited-outputs

This contains significant tissue-enhanced and -inhibited transcripts

Enhanced transcripts have a positive log2fold change. Inhibited transcripts have a nehative log2fold change.

Unique (i.e. species-specific) tissue-enhanced transcripts

Data for both species are in the directory Unique-tissue-enhanced-outputs

This contains significant tissue-enhanced transcripts per species - they contain the gene symbol for the annotation.

Differentially Expressed Genes (DEGs)

Contains the per tissue DEGs between the Emperor Penguin and King penguin.

NB:

Overexpressed genes in the Emperor penguin have a negative log2fold change

Underexpressed genes in the Emperor penguin have a positive log2fold change