diff --git a/README.md b/README.md
index 08e65f73..bcddddb3 100644
--- a/README.md
+++ b/README.md
@@ -71,6 +71,7 @@ If you've come here to help contribute - Thanks! Take a look at the [contributin
   - [Mock](#mock)
     - [.toHaveBeenCalledBefore()](#tohavebeencalledbefore)
     - [.toHaveBeenCalledAfter()](#tohavebeencalledafter)
+    - [.toHaveBeenCalledOnce()](#tohavebeencalledonce)
   - [Number](#number)
     - [.toBeNumber()](#tobenumber)
     - [.toBeNaN()](#tobenan)
@@ -533,6 +534,20 @@ it('calls mock1 after mock2', () => {
 });
 ```
 
+#### .toHaveBeenCalledOnce()
+
+Use `.toHaveBeenCalledOnce` to check if a `Mock` was called exactly one time.
+
+```js
+it('passes only if mock was called exactly once', () => {
+  const mock = jest.fn();
+
+  expect(mock).not.toHaveBeenCalled();
+  mock();
+  expect(mock).toHaveBeenCalledOnce();
+});
+```
+
 ### Number
 
 #### .toBeNumber()
diff --git a/src/matchers/index.js b/src/matchers/index.js
index 4ab5fc11..7d15a604 100644
--- a/src/matchers/index.js
+++ b/src/matchers/index.js
@@ -47,6 +47,7 @@ import toEndWithMatcher from './toEndWith';
 import toEqualCaseInsensitiveMatcher from './toEqualCaseInsensitive';
 import toHaveBeenCalledAfterMatcher from './toHaveBeenCalledAfter';
 import toHaveBeenCalledBeforeMatcher from './toHaveBeenCalledBefore';
+import toHaveBeenCalledOnceMatcher from './toHaveBeenCalledOnce';
 import toIncludeMatcher from './toInclude';
 import toIncludeAllMembersMatcher from './toIncludeAllMembers';
 import toIncludeAllPartialMembersMatcher from './toIncludeAllPartialMembers';
@@ -113,6 +114,7 @@ export const toEndWith = toEndWithMatcher.toEndWith;
 export const toEqualCaseInsensitive = toEqualCaseInsensitiveMatcher.toEqualCaseInsensitive;
 export const toHaveBeenCalledAfter = toHaveBeenCalledAfterMatcher.toHaveBeenCalledAfter;
 export const toHaveBeenCalledBefore = toHaveBeenCalledBeforeMatcher.toHaveBeenCalledBefore;
+export const toHaveBeenCalledOnce = toHaveBeenCalledOnceMatcher.toHaveBeenCalledOnce;
 export const toInclude = toIncludeMatcher.toInclude;
 export const toIncludeAllMembers = toIncludeAllMembersMatcher.toIncludeAllMembers;
 export const toIncludeAllPartialMembers = toIncludeAllPartialMembersMatcher.toIncludeAllPartialMembers;
diff --git a/src/matchers/toHaveBeenCalledOnce/__snapshots__/index.test.js.snap b/src/matchers/toHaveBeenCalledOnce/__snapshots__/index.test.js.snap
new file mode 100644
index 00000000..9079ad90
--- /dev/null
+++ b/src/matchers/toHaveBeenCalledOnce/__snapshots__/index.test.js.snap
@@ -0,0 +1,30 @@
+// Jest Snapshot v1, https://goo.gl/fbAQLP
+
+exports[`.not.toHaveBeenCalledOnce fails if mock was invoked exactly once 1`] = `
+"<dim>expect(</><red>received</><dim>).not.toHaveBeenCalledOnce(</><green>expected</><dim>)</>
+
+Expected mock function to have been called any amount of times but one, but it was called exactly once."
+`;
+
+exports[`.toHaveBeenCalledOnce fails if mock was invoked more than once, indicating how many times it was invoked 1`] = `
+"<dim>expect(</><red>received</><dim>).toHaveBeenCalledOnce(</><green>expected</><dim>)</>
+
+Expected mock function to have been called exactly once, but it was called:
+  <red>17</> times"
+`;
+
+exports[`.toHaveBeenCalledOnce fails if mock was never invoked indicating that it was invoked 0 times 1`] = `
+"<dim>expect(</><red>received</><dim>).toHaveBeenCalledOnce(</><green>expected</><dim>)</>
+
+Expected mock function to have been called exactly once, but it was called:
+  <red>0</> times"
+`;
+
+exports[`.toHaveBeenCalledOnce fails when given value is not a jest spy or mock 1`] = `
+"<dim>expect(</><red>received</><dim>).toHaveBeenCalledAfter(</><green>expected</><dim>)</>
+
+Matcher error: <red>\\"received\\"</> must be a mock or spy function
+
+Received has type:  function
+Received has value: <red>[Function mock1]</>"
+`;
diff --git a/src/matchers/toHaveBeenCalledOnce/index.js b/src/matchers/toHaveBeenCalledOnce/index.js
new file mode 100644
index 00000000..1e2f5647
--- /dev/null
+++ b/src/matchers/toHaveBeenCalledOnce/index.js
@@ -0,0 +1,45 @@
+import { matcherHint, printReceived, printWithType } from 'jest-matcher-utils';
+
+import { isJestMockOrSpy } from '../../utils';
+
+import predicate from './predicate';
+
+const passMessage = () => () =>
+  matcherHint('.not.toHaveBeenCalledOnce') +
+  '\n\n' +
+  'Expected mock function to have been called any amount of times but one, but it was called exactly once.';
+
+const failMessage = mockFn => () => {
+  return (
+    matcherHint('.toHaveBeenCalledOnce') +
+    '\n\n' +
+    'Expected mock function to have been called exactly once, but it was called:\n' +
+    `  ${printReceived(mockFn.mock.calls.length)} times`
+  );
+};
+
+const mockCheckFailMessage = value => () => {
+  return (
+    matcherHint('.toHaveBeenCalledAfter') +
+    '\n\n' +
+    `Matcher error: ${printReceived('received')} must be a mock or spy function` +
+    '\n\n' +
+    printWithType('Received', value, printReceived)
+  );
+};
+
+export default {
+  toHaveBeenCalledOnce: received => {
+    if (!isJestMockOrSpy(received)) {
+      return { pass: false, message: mockCheckFailMessage(received) };
+    }
+
+    const pass = predicate(received);
+
+    return {
+      pass,
+      message: pass ? passMessage(received) : failMessage(received),
+      actual: received,
+    };
+  },
+};
diff --git a/src/matchers/toHaveBeenCalledOnce/index.test.js b/src/matchers/toHaveBeenCalledOnce/index.test.js
new file mode 100644
index 00000000..dd2328d8
--- /dev/null
+++ b/src/matchers/toHaveBeenCalledOnce/index.test.js
@@ -0,0 +1,52 @@
+import matcher from './';
+
+expect.extend(matcher);
+
+describe('.toHaveBeenCalledOnce', () => {
+  let mock;
+  beforeEach(() => {
+    mock = jest.fn();
+  });
+
+  test('passes if mock was invoked exactly once', () => {
+    mock();
+    expect(mock).toHaveBeenCalledOnce();
+  });
+
+  test('fails if mock was never invoked indicating that it was invoked 0 times', () => {
+    expect(() => expect(mock).toHaveBeenCalledOnce()).toThrowErrorMatchingSnapshot();
+  });
+
+  test('fails if mock was invoked more than once, indicating how many times it was invoked', () => {
+    // Invoke mock 17 times
+    new Array(17).fill(mock).forEach(e => e(Math.random()));
+    expect(() => expect(mock).toHaveBeenCalledOnce()).toThrowErrorMatchingSnapshot();
+  });
+
+  test('fails when given value is not a jest spy or mock', () => {
+    const mock1 = () => {};
+    expect(() => expect(mock1).toHaveBeenCalledOnce()).toThrowErrorMatchingSnapshot();
+  });
+});
+
+describe('.not.toHaveBeenCalledOnce', () => {
+  let mock;
+  beforeEach(() => {
+    mock = jest.fn();
+  });
+
+  test('passes if mock was never invoked', () => {
+    expect(mock).not.toHaveBeenCalledOnce();
+  });
+
+  test('passes if mock was invoked more than once', () => {
+    mock();
+    mock();
+    expect(mock).not.toHaveBeenCalledOnce();
+  });
+
+  test('fails if mock was invoked exactly once', () => {
+    mock();
+    expect(() => expect(mock).not.toHaveBeenCalledOnce()).toThrowErrorMatchingSnapshot();
+  });
+});
diff --git a/src/matchers/toHaveBeenCalledOnce/predicate.js b/src/matchers/toHaveBeenCalledOnce/predicate.js
new file mode 100644
index 00000000..df139ad0
--- /dev/null
+++ b/src/matchers/toHaveBeenCalledOnce/predicate.js
@@ -0,0 +1 @@
+export default mockFn => mockFn.mock.calls.length === 1;
diff --git a/src/matchers/toHaveBeenCalledOnce/predicate.test.js b/src/matchers/toHaveBeenCalledOnce/predicate.test.js
new file mode 100644
index 00000000..ba5d9e5d
--- /dev/null
+++ b/src/matchers/toHaveBeenCalledOnce/predicate.test.js
@@ -0,0 +1,25 @@
+import predicate from './predicate';
+
+describe('.toHaveBeenCalledOnce predicate', () => {
+  let mock;
+  beforeEach(() => {
+    // Refresh on each test
+    mock = jest.fn();
+  });
+
+  test('returns true if mock was invoked exactly once', () => {
+    mock();
+    expect(predicate(mock)).toBe(true);
+  });
+
+  test('returns true if mock was invoked any amount of times but one', () => {
+    expect(predicate(mock)).toBe(false);
+
+    mock();
+    mock();
+    expect(predicate(mock)).toBe(false);
+
+    new Array(20).fill(mock).forEach(e => e());
+    expect(predicate(mock)).toBe(false);
+  });
+});
diff --git a/types/index.d.ts b/types/index.d.ts
index 82cada72..6680c133 100644
--- a/types/index.d.ts
+++ b/types/index.d.ts
@@ -162,6 +162,11 @@ declare namespace jest {
      */
     toHaveBeenCalledAfter(mock: jest.Mock): R;
 
+    /**
+     * Use `.toHaveBeenCalledOnce` to check if a `Mock` was called exactly one time.
+     */
+    toHaveBeenCalledOnce(): R;
+
     /**
      * Use `.toBeNumber` when checking if a value is a `Number`.
      */
@@ -541,6 +546,11 @@ declare namespace jest {
      */
     toHaveBeenCalledAfter(mock: jest.Mock): any;
 
+    /**
+     * Use `.toHaveBeenCalledOnce` to check if a `Mock` was called exactly one time.
+     */
+    toHaveBeenCalledOnce(): any;
+
     /**
      * Use `.toBeNumber` when checking if a value is a `Number`.
      */