diff --git a/packages/react-native/Libraries/AppDelegate/RCTAppDelegate.mm b/packages/react-native/Libraries/AppDelegate/RCTAppDelegate.mm
index 55bd24452b9b05..e2d9426be5b5d6 100644
--- a/packages/react-native/Libraries/AppDelegate/RCTAppDelegate.mm
+++ b/packages/react-native/Libraries/AppDelegate/RCTAppDelegate.mm
@@ -87,7 +87,7 @@ - (BOOL)application:(UIApplication *)application didFinishLaunchingWithOptions:(
 
   NSDictionary *initProps = updateInitialProps([self prepareInitialProps], fabricEnabled);
 
-  RCTAppSetupPrepareApp(application, enableTM, *_reactNativeConfig);
+  RCTAppSetupPrepareApp(application, enableTM);
 
   UIView *rootView;
   if (enableBridgeless) {
diff --git a/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.h b/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.h
index ad48fa19bcbf1c..8c5776140e5aa3 100644
--- a/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.h
+++ b/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.h
@@ -11,8 +11,6 @@
 
 #ifdef __cplusplus
 
-#import <react/config/ReactNativeConfig.h>
-
 #import <memory>
 
 #if USE_HERMES
@@ -43,21 +41,11 @@ std::unique_ptr<facebook::react::JSExecutorFactory> RCTAppSetupJsExecutorFactory
     RCTBridge *bridge,
     const std::shared_ptr<facebook::react::RuntimeScheduler> &runtimeScheduler);
 
-/**
- * Register features and experiments prior to app initialization.
- */
-void RCTAppSetupPrepareApp(
-    UIApplication *application,
-    BOOL turboModuleEnabled,
-    const facebook::react::ReactNativeConfig &reactNativeConfig);
-
 #endif // __cplusplus
 
 RCT_EXTERN_C_BEGIN
 
-void RCTAppSetupPrepareApp(UIApplication *application, BOOL turboModuleEnabled)
-    __deprecated_msg("Use the 3-argument overload of RCTAppSetupPrepareApp instead");
-
+void RCTAppSetupPrepareApp(UIApplication *application, BOOL turboModuleEnabled);
 UIView *RCTAppSetupDefaultRootView(
     RCTBridge *bridge,
     NSString *moduleName,
diff --git a/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.mm b/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.mm
index 865cac04ff4a5b..7c57844b1b272b 100644
--- a/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.mm
+++ b/packages/react-native/Libraries/AppDelegate/RCTAppSetupUtils.mm
@@ -24,9 +24,6 @@
 #import <React/RCTFabricSurface.h>
 #import <React/RCTSurfaceHostingProxyRootView.h>
 
-// jsinspector-modern
-#import <jsinspector-modern/InspectorFlags.h>
-
 void RCTAppSetupPrepareApp(UIApplication *application, BOOL turboModuleEnabled)
 {
   RCTEnableTurboModule(turboModuleEnabled);
@@ -38,20 +35,6 @@ void RCTAppSetupPrepareApp(UIApplication *application, BOOL turboModuleEnabled)
 #endif
 }
 
-void RCTAppSetupPrepareApp(
-    UIApplication *application,
-    BOOL turboModuleEnabled,
-    const facebook::react::ReactNativeConfig &reactNativeConfig)
-{
-#pragma clang diagnostic push
-#pragma clang diagnostic ignored "-Wdeprecated-declarations"
-  RCTAppSetupPrepareApp(application, turboModuleEnabled);
-#pragma clang diagnostic pop
-
-  auto &inspectorFlags = facebook::react::jsinspector_modern::InspectorFlags::getInstance();
-  inspectorFlags.initFromConfig(reactNativeConfig);
-}
-
 UIView *
 RCTAppSetupDefaultRootView(RCTBridge *bridge, NSString *moduleName, NSDictionary *initialProperties, BOOL fabricEnabled)
 {
diff --git a/packages/react-native/Libraries/AppDelegate/React-RCTAppDelegate.podspec b/packages/react-native/Libraries/AppDelegate/React-RCTAppDelegate.podspec
index 47eaade4f414f0..35281f25c003ea 100644
--- a/packages/react-native/Libraries/AppDelegate/React-RCTAppDelegate.podspec
+++ b/packages/react-native/Libraries/AppDelegate/React-RCTAppDelegate.podspec
@@ -88,7 +88,6 @@ Pod::Spec.new do |s|
   add_dependency(s, "React-utils")
   add_dependency(s, "React-debug")
   add_dependency(s, "React-rendererdebug")
-  add_dependency(s, "React-jsinspector")
 
   if use_hermes
     s.dependency "React-hermes"