diff --git a/Dockerfile b/Dockerfile
index 1557419209390..61d64212b402a 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -13,7 +13,7 @@ ENV \
 ARG QEMU_CPU
 
 # Install uv
-RUN pip3 install uv==0.5.0
+RUN pip3 install uv==0.5.4
 
 WORKDIR /usr/src
 
diff --git a/homeassistant/package_constraints.txt b/homeassistant/package_constraints.txt
index 40232ffb24c55..765fbd89a2440 100644
--- a/homeassistant/package_constraints.txt
+++ b/homeassistant/package_constraints.txt
@@ -65,7 +65,7 @@ standard-telnetlib==3.13.0;python_version>='3.13'
 typing-extensions>=4.12.2,<5.0
 ulid-transform==1.0.2
 urllib3>=1.26.5,<2
-uv==0.5.0
+uv==0.5.4
 voluptuous-openapi==0.0.5
 voluptuous-serialize==2.6.0
 voluptuous==0.15.2
diff --git a/pyproject.toml b/pyproject.toml
index 6b0b920678ca2..e281a2429d05d 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -75,7 +75,7 @@ dependencies    = [
     # Temporary setting an upper bound, to prevent compat issues with urllib3>=2
     # https://github.com/home-assistant/core/issues/97248
     "urllib3>=1.26.5,<2",
-    "uv==0.5.0",
+    "uv==0.5.4",
     "voluptuous==0.15.2",
     "voluptuous-serialize==2.6.0",
     "voluptuous-openapi==0.0.5",
diff --git a/requirements.txt b/requirements.txt
index ba4ed56a7a042..5ca035921078d 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -43,7 +43,7 @@ standard-telnetlib==3.13.0;python_version>='3.13'
 typing-extensions>=4.12.2,<5.0
 ulid-transform==1.0.2
 urllib3>=1.26.5,<2
-uv==0.5.0
+uv==0.5.4
 voluptuous==0.15.2
 voluptuous-serialize==2.6.0
 voluptuous-openapi==0.0.5
diff --git a/script/hassfest/docker/Dockerfile b/script/hassfest/docker/Dockerfile
index 89b10fa3027b5..b75477820ffeb 100644
--- a/script/hassfest/docker/Dockerfile
+++ b/script/hassfest/docker/Dockerfile
@@ -14,7 +14,7 @@ WORKDIR "/github/workspace"
 COPY . /usr/src/homeassistant
 
 # Uv is only needed during build
-RUN --mount=from=ghcr.io/astral-sh/uv:0.5.0,source=/uv,target=/bin/uv \
+RUN --mount=from=ghcr.io/astral-sh/uv:0.5.4,source=/uv,target=/bin/uv \
     # Required for PyTurboJPEG
     apk add --no-cache libturbojpeg \
     && uv pip install \