diff --git a/.changelog/32759.txt b/.changelog/32759.txt
new file mode 100644
index 00000000000..0b361006143
--- /dev/null
+++ b/.changelog/32759.txt
@@ -0,0 +1,7 @@
+```release-note:enhancement
+resource/aws_instance: Add `http_protocol_ipv6` attribute to `metadata_options` configuration block
+```
+
+```release-note:enhancement
+data-source/aws_instance: Add `metadata_options.http_protocol_ipv6` attribute
+```
\ No newline at end of file
diff --git a/internal/service/ec2/ec2_instance.go b/internal/service/ec2/ec2_instance.go
index 172dda9f050..412283ebf43 100644
--- a/internal/service/ec2/ec2_instance.go
+++ b/internal/service/ec2/ec2_instance.go
@@ -546,6 +546,12 @@ func ResourceInstance() *schema.Resource {
 							Default:      ec2.InstanceMetadataEndpointStateEnabled,
 							ValidateFunc: validation.StringInSlice(ec2.InstanceMetadataEndpointState_Values(), false),
 						},
+						"http_protocol_ipv6": {
+							Type:         schema.TypeString,
+							Optional:     true,
+							Default:      ec2.InstanceMetadataProtocolStateDisabled,
+							ValidateFunc: validation.StringInSlice(ec2.InstanceMetadataProtocolState_Values(), false),
+						},
 						"http_put_response_hop_limit": {
 							Type:         schema.TypeInt,
 							Optional:     true,
@@ -1813,6 +1819,7 @@ func resourceInstanceUpdate(ctx context.Context, d *schema.ResourceData, meta in
 
 				if tfMap["http_endpoint"].(string) == ec2.InstanceMetadataEndpointStateEnabled {
 					// These parameters are not allowed unless HttpEndpoint is enabled.
+					input.HttpProtocolIpv6 = aws.String(tfMap["http_protocol_ipv6"].(string))
 					input.HttpPutResponseHopLimit = aws.Int64(int64(tfMap["http_put_response_hop_limit"].(int)))
 					input.HttpTokens = aws.String(tfMap["http_tokens"].(string))
 					input.InstanceMetadataTags = aws.String(tfMap["instance_metadata_tags"].(string))
@@ -3100,6 +3107,9 @@ func expandInstanceMetadataOptions(l []interface{}) *ec2.InstanceMetadataOptions
 
 	if m["http_endpoint"].(string) == ec2.InstanceMetadataEndpointStateEnabled {
 		// These parameters are not allowed unless HttpEndpoint is enabled
+		if v, ok := m["http_protocol_ipv6"].(string); ok && v != "" {
+			opts.HttpProtocolIpv6 = aws.String(v)
+		}
 
 		if v, ok := m["http_tokens"].(string); ok && v != "" {
 			opts.HttpTokens = aws.String(v)
@@ -3176,6 +3186,7 @@ func flattenInstanceMetadataOptions(opts *ec2.InstanceMetadataOptionsResponse) [
 
 	m := map[string]interface{}{
 		"http_endpoint":               aws.StringValue(opts.HttpEndpoint),
+		"http_protocol_ipv6":          aws.StringValue(opts.HttpProtocolIpv6),
 		"http_put_response_hop_limit": aws.Int64Value(opts.HttpPutResponseHopLimit),
 		"http_tokens":                 aws.StringValue(opts.HttpTokens),
 		"instance_metadata_tags":      aws.StringValue(opts.InstanceMetadataTags),
diff --git a/internal/service/ec2/ec2_instance_data_source.go b/internal/service/ec2/ec2_instance_data_source.go
index 73e54d824aa..b56374c6163 100644
--- a/internal/service/ec2/ec2_instance_data_source.go
+++ b/internal/service/ec2/ec2_instance_data_source.go
@@ -228,6 +228,10 @@ func DataSourceInstance() *schema.Resource {
 							Type:     schema.TypeString,
 							Computed: true,
 						},
+						"http_protocol_ipv6": {
+							Type:     schema.TypeString,
+							Computed: true,
+						},
 						"http_put_response_hop_limit": {
 							Type:     schema.TypeInt,
 							Computed: true,
diff --git a/internal/service/ec2/ec2_instance_data_source_test.go b/internal/service/ec2/ec2_instance_data_source_test.go
index 74b89de3e4c..5fc4aec3915 100644
--- a/internal/service/ec2/ec2_instance_data_source_test.go
+++ b/internal/service/ec2/ec2_instance_data_source_test.go
@@ -634,6 +634,7 @@ func TestAccEC2InstanceDataSource_metadataOptions(t *testing.T) {
 				Check: resource.ComposeTestCheckFunc(
 					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.#", resourceName, "metadata_options.#"),
 					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.0.http_endpoint", resourceName, "metadata_options.0.http_endpoint"),
+					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.0.http_protocol_ipv6", resourceName, "metadata_options.0.http_protocol_ipv6"),
 					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.0.http_tokens", resourceName, "metadata_options.0.http_tokens"),
 					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.0.http_put_response_hop_limit", resourceName, "metadata_options.0.http_put_response_hop_limit"),
 					resource.TestCheckResourceAttrPair(datasourceName, "metadata_options.0.instance_metadata_tags", resourceName, "metadata_options.0.instance_metadata_tags"),
diff --git a/internal/service/ec2/ec2_instance_test.go b/internal/service/ec2/ec2_instance_test.go
index 5c9da246688..059182a97cc 100644
--- a/internal/service/ec2/ec2_instance_test.go
+++ b/internal/service/ec2/ec2_instance_test.go
@@ -5007,6 +5007,7 @@ func TestAccEC2Instance_metadataOptions(t *testing.T) {
 					testAccCheckInstanceExists(ctx, resourceName, &v),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.#", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_endpoint", "enabled"),
+					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_protocol_ipv6", "disabled"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_tokens", "optional"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_put_response_hop_limit", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.instance_metadata_tags", "disabled"),
@@ -5018,6 +5019,7 @@ func TestAccEC2Instance_metadataOptions(t *testing.T) {
 					testAccCheckInstanceExists(ctx, resourceName, &v),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.#", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_endpoint", "disabled"),
+					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_protocol_ipv6", "disabled"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_tokens", "optional"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_put_response_hop_limit", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.instance_metadata_tags", "disabled"),
@@ -5029,6 +5031,7 @@ func TestAccEC2Instance_metadataOptions(t *testing.T) {
 					testAccCheckInstanceExists(ctx, resourceName, &v),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.#", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_endpoint", "enabled"),
+					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_protocol_ipv6", "enabled"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_tokens", "required"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_put_response_hop_limit", "2"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.instance_metadata_tags", "enabled"),
@@ -5040,6 +5043,7 @@ func TestAccEC2Instance_metadataOptions(t *testing.T) {
 					testAccCheckInstanceExists(ctx, resourceName, &v),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.#", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_endpoint", "enabled"),
+					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_protocol_ipv6", "disabled"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_tokens", "optional"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.http_put_response_hop_limit", "1"),
 					resource.TestCheckResourceAttr(resourceName, "metadata_options.0.instance_metadata_tags", "disabled"),
@@ -8542,6 +8546,7 @@ resource "aws_instance" "test" {
 
   metadata_options {
     http_endpoint               = "enabled"
+    http_protocol_ipv6          = "enabled"
     http_tokens                 = "required"
     http_put_response_hop_limit = 2
     instance_metadata_tags      = "enabled"
@@ -8567,6 +8572,7 @@ resource "aws_instance" "test" {
 
   metadata_options {
     http_endpoint               = "enabled"
+    http_protocol_ipv6          = "disabled"
     http_tokens                 = "optional"
     http_put_response_hop_limit = 1
     instance_metadata_tags      = "disabled"
diff --git a/website/docs/d/instance.html.markdown b/website/docs/d/instance.html.markdown
index 668e3a5e58a..38071202494 100644
--- a/website/docs/d/instance.html.markdown
+++ b/website/docs/d/instance.html.markdown
@@ -92,6 +92,7 @@ interpolation.
     * `auto_recovery` - Automatic recovery behavior of the instance.
 * `metadata_options` - Metadata options of the Instance.
     * `http_endpoint` - State of the metadata service: `enabled`, `disabled`.
+    * `http_protocol_ipv6` - Whether the IPv6 endpoint for the instance metadata service is `enabled` or `disabled`
     * `http_tokens` - If session tokens are required: `optional`, `required`.
     * `http_put_response_hop_limit` - Desired HTTP PUT response hop limit for instance metadata requests.
     * `instance_metadata_tags` - If access to instance tags is allowed from the metadata service: `enabled`, `disabled`.
diff --git a/website/docs/r/instance.html.markdown b/website/docs/r/instance.html.markdown
index 13f420074c5..fba0b71cef8 100644
--- a/website/docs/r/instance.html.markdown
+++ b/website/docs/r/instance.html.markdown
@@ -355,6 +355,7 @@ Metadata options can be applied/modified to the EC2 Instance at any time.
 The `metadata_options` block supports the following:
 
 * `http_endpoint` - (Optional) Whether the metadata service is available. Valid values include `enabled` or `disabled`. Defaults to `enabled`.
+* `http_protocol_ipv6` - (Optional) Whether the IPv6 endpoint for the instance metadata service is enabled. Defaults to `disabled`.
 * `http_put_response_hop_limit` - (Optional) Desired HTTP PUT response hop limit for instance metadata requests. The larger the number, the further instance metadata requests can travel. Valid values are integer from `1` to `64`. Defaults to `1`.
 * `http_tokens` - (Optional) Whether or not the metadata service requires session tokens, also referred to as _Instance Metadata Service Version 2 (IMDSv2)_. Valid values include `optional` or `required`. Defaults to `optional`.
 * `instance_metadata_tags` - (Optional) Enables or disables access to instance tags from the instance metadata service. Valid values include `enabled` or `disabled`. Defaults to `disabled`.