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nextflow.config
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/*
* -------------------------------------------------
* NanoRTax Nextflow config file
* -------------------------------------------------
* Default config options for all environments.
*/
// Global default params, used in configs
params {
// Workflow flags
reads = ""
outdir = './results'
min_read_length = 1400
max_read_length = 1700
taxonkit_db = "db/"
blast_db = "db/16S_ribosomal_RNA"
blast_taxdb = "db/taxdb"
kraken_db = "db/16S_RDP_k2db"
centrifuge_db = "db/silva"
cntrf_min_hitlen = 16
blast_evalue = 11
blast_max_hsps = 50
// Boilerplate options
name = false
help = false
monochrome_logs = false
// Defaults only, expecting to be overwritten
max_memory = 128.GB
max_cpus = 16
max_time = 240.h
}
// Container slug. Stable releases should specify release tag!
// Developmental code should specify :dev
process.container = 'hecrp/nanortax:latest'
// Load base.config by default for all pipelines
includeConfig 'conf/base.config'
profiles {
conda {
conda.enabled = true
process.conda = "$baseDir/environment.yml" }
debug { process.beforeScript = 'echo $HOSTNAME' }
docker {
docker.enabled = true
docker.temp = "$baseDir"
// Avoid this error:
// WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap.
// Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351
// once this is established and works well, nextflow might implement this behavior as new default.
docker.runOptions = '-u $(id -u):$(id -g)'
docker.fixOwnership = true
}
singularity {
singularity.enabled = true
singularity.autoMounts = true
}
test { includeConfig 'conf/test.config' }
}
// Export this variable to prevent local Python libraries from conflicting with those in the container
env {
PYTHONNOUSERSITE = 1
}
// Capture exit codes from upstream processes when piping
process.shell = ['/bin/bash', '-euo', 'pipefail']
manifest {
name = 'NanoRTax'
author = 'Laura Ciuffreda, Héctor Rodríguez Pérez'
homePage = 'https://github.com/genomicsiter/nanortax'
description = 'Real-time analysis pipeline for nanopore metagenomic data'
mainScript = 'main.nf'
nextflowVersion = '>=19.10.0'
version = '1.0dev'
}
// Function to ensure that resource requirements don't go beyond
// a maximum limit
def check_max(obj, type) {
if (type == 'memory') {
try {
if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
return params.max_memory as nextflow.util.MemoryUnit
else
return obj
} catch (all) {
println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'time') {
try {
if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
return params.max_time as nextflow.util.Duration
else
return obj
} catch (all) {
println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'cpus') {
try {
return Math.min( obj, params.max_cpus as int )
} catch (all) {
println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
return obj
}
}
}