diff --git a/.travis.yml b/.travis.yml
index d4dd786f4..6ed83d3f7 100644
--- a/.travis.yml
+++ b/.travis.yml
@@ -5,7 +5,6 @@ env:
   - TOX_ENV=py27-lint
   - TOX_ENV=py34-lint
   - TOX_ENV=py27-lint-readme
-  - TOX_ENV=py26
   - TOX_ENV=py27
   - TOX_ENV=py34
 
diff --git a/CONTRIBUTING.rst b/CONTRIBUTING.rst
index 2e484211a..a6cb11723 100644
--- a/CONTRIBUTING.rst
+++ b/CONTRIBUTING.rst
@@ -106,7 +106,7 @@ Before you submit a pull request, check that it meets these guidelines:
 
 1. If the pull request adds functionality, the docs should be updated. Put
    your new functionality into a function with a docstring.
-2. The pull request should work for Python 2.6 and 2.7. Check
+2. The pull request should work for Python 2.7 and 3.4. Check
    https://travis-ci.org/galaxyproject/planemo/pull_requests
    and make sure that the tests pass for all supported Python versions.
 
@@ -118,7 +118,7 @@ To run a subset of tests::
     % make tox ENV=py27 ARGS='--tests tests/test_shed_upload.py'
 
 This will use Tox_ to run the specified tests using Python 2.7. ``ENV`` here
-can be used to specify different Python version (e.g. ``py26``, ``py27``,
+can be used to specify different Python version (e.g. ``py27`` and
 ``py34``). Python 3.4 is a work in progress. 
 
 Even more granularity is also possible by specifying specific test methods.::
@@ -157,9 +157,6 @@ testing environment. Planemo defines the following environments:
 ``py27-lint-readme``
     Lint the README reStructuredText.
 
-``py26``
-    Run planemo tests in Python 2.6.
-
 ``py27``
     Run planemo tests in Python 2.7.
 
diff --git a/dev-requirements.txt b/dev-requirements.txt
index b43653962..a9b97dc8f 100644
--- a/dev-requirements.txt
+++ b/dev-requirements.txt
@@ -12,9 +12,6 @@ sphinx
 # Used to check readme.
 readme
 
-# For Python 2.6 tests
-unittest2
-
 # Used for code checking.
 pyflakes
 flake8
diff --git a/setup.py b/setup.py
index 6dbace4ed..424cb517d 100644
--- a/setup.py
+++ b/setup.py
@@ -4,11 +4,16 @@
 import ast
 import os
 import re
+import sys
 try:
     from setuptools import setup
 except ImportError:
     from distutils.core import setup
 
+if sys.version_info < (2, 7):
+    sys.stderr.write("ERROR: planemo requires at least Python Version 2.7\n")
+    sys.exit(1)
+
 SOURCE_DIR = "planemo"
 
 _version_re = re.compile(r'__version__\s+=\s+(.*)')
@@ -114,7 +119,6 @@ def get_var(var_name):
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 2",
-        'Programming Language :: Python :: 2.6',
         'Programming Language :: Python :: 2.7',
     ],
     test_suite=TEST_DIR,
diff --git a/tox.ini b/tox.ini
index 3bb38bb4a..543d7eeb9 100644
--- a/tox.ini
+++ b/tox.ini
@@ -1,7 +1,7 @@
 # TODO: py34 to envlist
 # TODO: implement doc linting
 [tox]
-envlist = py34-lint, py27-lint, py27-lint-readme, py26, py27
+envlist = py34-lint, py27-lint, py27-lint-readme, py27
 source_dir = planemo
 test_dir = tests
 
@@ -11,7 +11,6 @@ commands = {envpython} setup.py nosetests []
 deps =
     -rrequirements.txt
     py27: cwltool
-    py26: unittest2
     nose
     coverage
     flask