You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if fgbio can reverse-complement a read structure, for example, when the type is in lower case.
Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?
The text was updated successfully, but these errors were encountered:
I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if
fgbio
can reverse-complement a read structure, for example, when the type is in lower case.Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?
The text was updated successfully, but these errors were encountered: