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Extend Read Structures operators for reverse-complemented structures #949

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galaxy001 opened this issue Nov 13, 2023 · 0 comments
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@galaxy001
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galaxy001 commented Nov 13, 2023

I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if fgbio can reverse-complement a read structure, for example, when the type is in lower case.

Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?

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