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Apo_Holo_Analysis

Please cite: Wankowicz, S. A., de Oliveira, S. H., Hogan, D. W., van den Bedem, H., & Fraser, J. S. (2022). Ligand binding remodels protein side chain conformational heterogeneity. Elife, 11, e74114.

The scripts in this repository are related to the manuscript Ligand binding remodels protein side chain conformational heterogeneity.

A corresponding blog post about tricks and tips about running a structural bioinformatic pipeline can be found here.

Scripts are organized into four categories:

  1. PDB Selection
  2. Refinement, Creation of Multiconformer Models
  3. Quality Control
  4. Analysis

Inside each folder is another README that describes how to use the script/pipeline in each folder.

Dependencies:

qFit-3.0

Phenix

Python Dependencies (recommended to install in same conda env as qFit. See qFit-3.0 for more details).

numpy pandas Biopython glob

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Scripts for paper on Apo/Holo Pairs

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