diff --git a/src/Compilers/Test/Core/ModuleInitializer.cs b/src/Compilers/Test/Core/ModuleInitializer.cs
index 6e592c35225c8..2b1bcb4d22081 100644
--- a/src/Compilers/Test/Core/ModuleInitializer.cs
+++ b/src/Compilers/Test/Core/ModuleInitializer.cs
@@ -17,10 +17,6 @@ internal static void Initialize()
         {
             Trace.Listeners.Clear();
             Trace.Listeners.Add(new ThrowingTraceListener());
-
-            // Make sure we load DSRN from the directory containing the unit tests and not from a runtime directory on .NET 5+.
-            Environment.SetEnvironmentVariable("MICROSOFT_DIASYMREADER_NATIVE_ALT_LOAD_PATH", Path.GetDirectoryName(typeof(ModuleInitializer).Assembly.Location));
-            Environment.SetEnvironmentVariable("MICROSOFT_DIASYMREADER_NATIVE_USE_ALT_LOAD_PATH_ONLY", "1");
         }
     }
 }