diff --git a/eng/common/templates/job/publish-build-assets.yml b/eng/common/templates/job/publish-build-assets.yml
index 3b9e2524ff3..fe9dfdf720c 100644
--- a/eng/common/templates/job/publish-build-assets.yml
+++ b/eng/common/templates/job/publish-build-assets.yml
@@ -94,7 +94,31 @@ jobs:
         PathtoPublish: '$(Build.StagingDirectory)/ReleaseConfigs.txt'
         PublishLocation: Container
         ArtifactName: ReleaseConfigs
-    
+
+    - task: powershell@2
+      displayName: Check if SymbolPublishingExclusionsFile.txt exists
+      inputs:
+        targetType: inline
+        script: |
+          $symbolExclusionfile = "$(Build.SourcesDirectory)/eng/SymbolPublishingExclusionsFile.txt"
+          if(Test-Path -Path $symbolExclusionfile)
+          {
+            Write-Host "SymbolExclusionFile exists"
+            Write-Host "##vso[task.setvariable variable=SymbolExclusionFile]true"
+          }
+          else{
+           Write-Host "Symbols Exclusion file does not exists"
+           Write-Host "##vso[task.setvariable variable=SymbolExclusionFile]false"
+          }
+
+    - task: PublishBuildArtifacts@1
+      displayName: Publish SymbolPublishingExclusionsFile Artifact
+      condition: eq(variables['SymbolExclusionFile'], 'true') 
+      inputs:
+        PathtoPublish: '$(Build.SourcesDirectory)/eng/SymbolPublishingExclusionsFile.txt'
+        PublishLocation: Container
+        ArtifactName: ReleaseConfigs
+        
     - ${{ if eq(parameters.enablePublishBuildArtifacts, 'true') }}:
       - template: /eng/common/templates/steps/publish-logs.yml
         parameters:
diff --git a/eng/publishing/v3/publish-assets.yml b/eng/publishing/v3/publish-assets.yml
index 33ef36c0979..60dca2adcde 100644
--- a/eng/publishing/v3/publish-assets.yml
+++ b/eng/publishing/v3/publish-assets.yml
@@ -43,6 +43,7 @@ jobs:
           AssetManifests/**
           BlobArtifacts/MergedManifest.xml
           PdbArtifacts/**
+          ReleaseConfigs/SymbolPublishingExclusionsFile.txt
         downloadPath: '$(Build.ArtifactStagingDirectory)'
 
     - task: NuGetToolInstaller@1
@@ -84,7 +85,7 @@ jobs:
           /p:AkaMSClientSecret=$(akams-client-secret)
           ${{ parameters.artifactsPublishingAdditionalParameters }}
           /p:PDBArtifactsBasePath='$(Build.ArtifactStagingDirectory)/PDBArtifacts/'
-          /p:SymbolPublishingExclusionsFile='$(Build.SourcesDirectory)/eng/SymbolPublishingExclusionsFile.txt'
+          /p:SymbolPublishingExclusionsFile='$(Build.ArtifactStagingDirectory)/ReleaseConfigs/SymbolPublishingExclusionsFile.txt'
           ${{ parameters.symbolPublishingAdditionalParameters}}
           /p:MsdlToken=$(microsoft-symbol-server-pat)
           /p:SymWebToken=$(symweb-symbol-server-pat)
diff --git a/src/Microsoft.DotNet.Build.Tasks.Feed/src/PublishArtifactsInManifestBase.cs b/src/Microsoft.DotNet.Build.Tasks.Feed/src/PublishArtifactsInManifestBase.cs
index 986f2668445..b626d27ef90 100644
--- a/src/Microsoft.DotNet.Build.Tasks.Feed/src/PublishArtifactsInManifestBase.cs
+++ b/src/Microsoft.DotNet.Build.Tasks.Feed/src/PublishArtifactsInManifestBase.cs
@@ -426,6 +426,27 @@ public async Task PublishSymbolsUsingStreamingAsync(
             HashSet<TargetFeedConfig> feedConfigsForSymbols = FeedConfigs[symbolCategory];
             Dictionary<string, string> serversToPublish =
                 GetTargetSymbolServers(feedConfigsForSymbols, msdlToken, symWebToken);
+            HashSet<string> excludeFiles = null;
+            
+            if(File.Exists(symbolPublishingExclusionsFile))
+            {
+                Log.LogMessage(MessageImportance.Normal, $"SymbolPublishingExclusionFile exists");
+                string[] files = File.ReadAllLines(symbolPublishingExclusionsFile);
+                excludeFiles = new HashSet<string>();
+
+                foreach(var file in files)
+                {
+                    if(!string.IsNullOrEmpty(file))
+                    {
+                        Log.LogMessage(MessageImportance.Normal, $"Exclude the file {file} from publishing to symbol server");
+                        excludeFiles.Add(file);
+                    }
+                }
+            }
+            else
+            {
+                Log.LogMessage(MessageImportance.Normal, $"SymbolPublishingExclusionFile was not found at ${symbolPublishingExclusionsFile} ");
+            }
 
             if (symbolsToPublish != null && symbolsToPublish.Any())
             {
@@ -471,7 +492,7 @@ await PublishSymbolsHelper.PublishAsync(
                                         token,
                                         symbolFiles,
                                         null,
-                                        null,
+                                        excludeFiles,
                                         ExpirationInDays,
                                         false,
                                         publishSpecialClrFiles,
@@ -537,7 +558,7 @@ await PublishSymbolsHelper.PublishAsync(
                             token,
                             null,
                             filesToSymbolServer,
-                            null,
+                            excludeFiles,
                             ExpirationInDays,
                             false,
                             publishSpecialClrFiles,