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bamtk.c
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bamtk.c
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#include <stdio.h>
#include <unistd.h>
#include <assert.h>
#include <fcntl.h>
#include "bam.h"
#ifdef _USE_KNETFILE
#include "knetfile.h"
#endif
int bam_taf2baf(int argc, char *argv[]);
int bam_mpileup(int argc, char *argv[]);
int bam_merge(int argc, char *argv[]);
int bam_index(int argc, char *argv[]);
int bam_sort(int argc, char *argv[]);
int bam_tview_main(int argc, char *argv[]);
int bam_mating(int argc, char *argv[]);
int bam_rmdup(int argc, char *argv[]);
int bam_flagstat(int argc, char *argv[]);
int bam_fillmd(int argc, char *argv[]);
int bam_idxstats(int argc, char *argv[]);
int main_samview(int argc, char *argv[]);
int main_import(int argc, char *argv[]);
int main_reheader(int argc, char *argv[]);
int main_cut_target(int argc, char *argv[]);
int main_phase(int argc, char *argv[]);
int main_cat(int argc, char *argv[]);
int main_depth(int argc, char *argv[]);
int main_bam2fq(int argc, char *argv[]);
int main_pad2unpad(int argc, char *argv[]);
int main_bedcov(int argc, char *argv[]);
int main_bamshuf(int argc, char *argv[]);
int faidx_main(int argc, char *argv[]);
static int usage()
{
fprintf(stderr, "\n");
fprintf(stderr, "Program: samtools (Tools for alignments in the SAM format)\n");
fprintf(stderr, "Version: %s\n\n", BAM_VERSION);
fprintf(stderr, "Usage: samtools <command> [options]\n\n");
fprintf(stderr, "Command: view SAM<->BAM conversion\n");
fprintf(stderr, " sort sort alignment file\n");
fprintf(stderr, " mpileup multi-way pileup\n");
fprintf(stderr, " depth compute the depth\n");
fprintf(stderr, " faidx index/extract FASTA\n");
#if _CURSES_LIB != 0
fprintf(stderr, " tview text alignment viewer\n");
#endif
fprintf(stderr, " index index alignment\n");
fprintf(stderr, " idxstats BAM index stats (r595 or later)\n");
fprintf(stderr, " fixmate fix mate information\n");
fprintf(stderr, " flagstat simple stats\n");
fprintf(stderr, " calmd recalculate MD/NM tags and '=' bases\n");
fprintf(stderr, " merge merge sorted alignments\n");
fprintf(stderr, " rmdup remove PCR duplicates\n");
fprintf(stderr, " reheader replace BAM header\n");
fprintf(stderr, " cat concatenate BAMs\n");
fprintf(stderr, " bedcov read depth per BED region\n");
fprintf(stderr, " targetcut cut fosmid regions (for fosmid pool only)\n");
fprintf(stderr, " phase phase heterozygotes\n");
fprintf(stderr, " bamshuf shuffle and group alignments by name\n");
// fprintf(stderr, " depad convert padded BAM to unpadded BAM\n"); // not stable
fprintf(stderr, "\n");
#ifdef _WIN32
fprintf(stderr, "\
Note: The Windows version of SAMtools is mainly designed for read-only\n\
operations, such as viewing the alignments and generating the pileup.\n\
Binary files generated by the Windows version may be buggy.\n\n");
#endif
return 1;
}
int main(int argc, char *argv[])
{
#ifdef _WIN32
setmode(fileno(stdout), O_BINARY);
setmode(fileno(stdin), O_BINARY);
#ifdef _USE_KNETFILE
knet_win32_init();
#endif
#endif
if (argc < 2) return usage();
if (strcmp(argv[1], "view") == 0) return main_samview(argc-1, argv+1);
else if (strcmp(argv[1], "import") == 0) return main_import(argc-1, argv+1);
else if (strcmp(argv[1], "mpileup") == 0) return bam_mpileup(argc-1, argv+1);
else if (strcmp(argv[1], "merge") == 0) return bam_merge(argc-1, argv+1);
else if (strcmp(argv[1], "sort") == 0) return bam_sort(argc-1, argv+1);
else if (strcmp(argv[1], "index") == 0) return bam_index(argc-1, argv+1);
else if (strcmp(argv[1], "idxstats") == 0) return bam_idxstats(argc-1, argv+1);
else if (strcmp(argv[1], "faidx") == 0) return faidx_main(argc-1, argv+1);
else if (strcmp(argv[1], "fixmate") == 0) return bam_mating(argc-1, argv+1);
else if (strcmp(argv[1], "rmdup") == 0) return bam_rmdup(argc-1, argv+1);
else if (strcmp(argv[1], "flagstat") == 0) return bam_flagstat(argc-1, argv+1);
else if (strcmp(argv[1], "calmd") == 0) return bam_fillmd(argc-1, argv+1);
else if (strcmp(argv[1], "fillmd") == 0) return bam_fillmd(argc-1, argv+1);
else if (strcmp(argv[1], "reheader") == 0) return main_reheader(argc-1, argv+1);
else if (strcmp(argv[1], "cat") == 0) return main_cat(argc-1, argv+1);
else if (strcmp(argv[1], "targetcut") == 0) return main_cut_target(argc-1, argv+1);
else if (strcmp(argv[1], "phase") == 0) return main_phase(argc-1, argv+1);
else if (strcmp(argv[1], "depth") == 0) return main_depth(argc-1, argv+1);
else if (strcmp(argv[1], "bam2fq") == 0) return main_bam2fq(argc-1, argv+1);
else if (strcmp(argv[1], "pad2unpad") == 0) return main_pad2unpad(argc-1, argv+1);
else if (strcmp(argv[1], "depad") == 0) return main_pad2unpad(argc-1, argv+1);
else if (strcmp(argv[1], "bedcov") == 0) return main_bedcov(argc-1, argv+1);
else if (strcmp(argv[1], "bamshuf") == 0) return main_bamshuf(argc-1, argv+1);
else if (strcmp(argv[1], "pileup") == 0) {
fprintf(stderr, "[main] The `pileup' command has been removed. Please use `mpileup' instead.\n");
return 1;
}
#if _CURSES_LIB != 0
else if (strcmp(argv[1], "tview") == 0) return bam_tview_main(argc-1, argv+1);
#endif
else {
fprintf(stderr, "[main] unrecognized command '%s'\n", argv[1]);
return 1;
}
return 0;
}