diff --git a/cli/tsc/dts/lib.dom.d.ts b/cli/tsc/dts/lib.dom.d.ts
index 0a2f9b9edaa6e0..2684735597a37a 100644
--- a/cli/tsc/dts/lib.dom.d.ts
+++ b/cli/tsc/dts/lib.dom.d.ts
@@ -18277,7 +18277,7 @@ declare var ReadableStream: {
     new(underlyingSource: UnderlyingByteSource, strategy?: { highWaterMark?: number }): ReadableStream<Uint8Array>;
     new<R = any>(underlyingSource: UnderlyingDefaultSource<R>, strategy?: QueuingStrategy<R>): ReadableStream<R>;
     new<R = any>(underlyingSource?: UnderlyingSource<R>, strategy?: QueuingStrategy<R>): ReadableStream<R>;
-    from<R>(asyncIterable: AsyncIterable<R> | Iterable<R | PromiseLike<R>>): ReadableStream<R>;
+    from<R>(asyncIterable: AsyncIterable<R> | Iterable<R | PromiseLike<R>> & object): ReadableStream<R>;
 };
 
 /** [MDN Reference](https://developer.mozilla.org/docs/Web/API/ReadableStreamBYOBReader) */
diff --git a/ext/web/06_streams.js b/ext/web/06_streams.js
index f29e5f204009be..57a437e4f5c304 100644
--- a/ext/web/06_streams.js
+++ b/ext/web/06_streams.js
@@ -2922,7 +2922,7 @@ function readableStreamPipeTo(
 }
 
 /**
- * @param {ReadableStreamGenericReader<any> | ReadableStreamBYOBReader} reader
+ * @param {ReadableStreamGenericReader | ReadableStreamBYOBReader} reader
  * @param {any} reason
  * @returns {Promise<void>}
  */
@@ -2955,7 +2955,7 @@ function readableStreamReaderGenericInitialize(reader, stream) {
 
 /**
  * @template R
- * @param {ReadableStreamGenericReader<R> | ReadableStreamBYOBReader} reader
+ * @param {ReadableStreamGenericReader | ReadableStreamBYOBReader} reader
  */
 function readableStreamReaderGenericRelease(reader) {
   const stream = reader[_stream];
diff --git a/ext/web/06_streams_types.d.ts b/ext/web/06_streams_types.d.ts
index e04f568d26644a..fe05ee6e65b167 100644
--- a/ext/web/06_streams_types.d.ts
+++ b/ext/web/06_streams_types.d.ts
@@ -60,8 +60,8 @@ interface VoidFunction {
   (): void;
 }
 
-interface ReadableStreamGenericReader<T> {
-  readonly closed: Promise<void>;
+interface ReadableStreamGenericReader {
+  readonly closed: Promise<undefined>;
   // deno-lint-ignore no-explicit-any
   cancel(reason?: any): Promise<void>;
 }
diff --git a/ext/web/lib.deno_web.d.ts b/ext/web/lib.deno_web.d.ts
index 2ad97ac7d1eef2..8aafbad535923a 100644
--- a/ext/web/lib.deno_web.d.ts
+++ b/ext/web/lib.deno_web.d.ts
@@ -882,7 +882,7 @@ declare var ReadableStream: {
     strategy?: QueuingStrategy<R>,
   ): ReadableStream<R>;
   from<R>(
-    asyncIterable: AsyncIterable<R> | Iterable<R | PromiseLike<R>>,
+    asyncIterable: AsyncIterable<R> | Iterable<R | PromiseLike<R>> & object,
   ): ReadableStream<R>;
 };
 
diff --git a/tests/specs/check/reject_string_in_readable_stream_from/__test__.jsonc b/tests/specs/check/reject_string_in_readable_stream_from/__test__.jsonc
new file mode 100644
index 00000000000000..634dbac8503ab5
--- /dev/null
+++ b/tests/specs/check/reject_string_in_readable_stream_from/__test__.jsonc
@@ -0,0 +1,5 @@
+{
+  "args": "check ./main.ts",
+  "output": "main.out",
+  "exitCode": 1
+}
diff --git a/tests/specs/check/reject_string_in_readable_stream_from/main.out b/tests/specs/check/reject_string_in_readable_stream_from/main.out
new file mode 100644
index 00000000000000..577c1fb059df7f
--- /dev/null
+++ b/tests/specs/check/reject_string_in_readable_stream_from/main.out
@@ -0,0 +1,5 @@
+Check [WILDCARD]/main.ts
+error: TS2345 [ERROR]: Argument of type 'string' is not assignable to parameter of type 'AsyncIterable<string> | (Iterable<string | PromiseLike<string>> & object)'.
+ReadableStream.from("string");
+                    ~~~~~~~~
+    at [WILDCARD]/main.ts:1:21
diff --git a/tests/specs/check/reject_string_in_readable_stream_from/main.ts b/tests/specs/check/reject_string_in_readable_stream_from/main.ts
new file mode 100644
index 00000000000000..5e7fc9c9563ec5
--- /dev/null
+++ b/tests/specs/check/reject_string_in_readable_stream_from/main.ts
@@ -0,0 +1 @@
+ReadableStream.from("string");
diff --git a/tests/unit/streams_test.ts b/tests/unit/streams_test.ts
index 73f9a60953c84b..53225a1553c6a3 100644
--- a/tests/unit/streams_test.ts
+++ b/tests/unit/streams_test.ts
@@ -541,6 +541,7 @@ Deno.test(async function decompressionStreamInvalidGzipStillReported() {
 
 Deno.test(function readableStreamFromWithStringThrows() {
   assertThrows(
+    // @ts-expect-error: primitives are not acceptable
     () => ReadableStream.from("string"),
     TypeError,
     "Failed to execute 'ReadableStream.from': Argument 1 can not be converted to async iterable.",