diff --git a/lib/compat/wordpress-5.9/script-loader.php b/lib/compat/wordpress-5.9/script-loader.php
index 1b091d0580b85..aea2275672598 100644
--- a/lib/compat/wordpress-5.9/script-loader.php
+++ b/lib/compat/wordpress-5.9/script-loader.php
@@ -5,30 +5,44 @@
  * @package gutenberg
  */
 
-/*
+/**
  * This code lives in script-loader.php
  * where we just load styles using wp_get_global_stylesheet.
  */
-if ( ! function_exists( 'wp_get_global_styles' ) ) {
-	/**
-	 * Fetches the preferences for each origin (core, theme, user)
-	 * and enqueues the resulting stylesheet.
+function gutenberg_enqueue_global_styles_assets() {
+	$separate_assets  = wp_should_load_separate_core_block_assets();
+	$is_block_theme   = wp_is_block_theme();
+	$is_classic_theme = ! $is_block_theme;
+
+	/*
+	 * Global styles should be printed in the head when loading all styles combined.
+	 * The footer should only be used to print global styles for classic themes with separate core assets enabled.
+	 *
+	 * See https://core.trac.wordpress.org/ticket/53494.
 	 */
-	function gutenberg_enqueue_global_styles_assets() {
-		$stylesheet = wp_get_global_stylesheet();
-		if ( empty( $stylesheet ) ) {
-			return;
-		}
+	if (
+		( $is_block_theme && doing_action( 'wp_footer' ) ) ||
+		( $is_classic_theme && doing_action( 'wp_footer' ) && ! $separate_assets ) ||
+		( $is_classic_theme && doing_action( 'wp_enqueue_scripts' ) && $separate_assets )
+	) {
+		return;
+	}
+
+	$stylesheet = wp_get_global_stylesheet();
+
+	if ( empty( $stylesheet ) ) {
+		return;
+	}
 
-		if ( isset( wp_styles()->registered['global-styles'] ) ) {
-			// We're in WordPress 5.8, so we overwrite the existing.
-			wp_styles()->registered['global-styles']->extra['after'][0] = $stylesheet;
-		} else {
-			// WordPress 5.7 or lower.
-			wp_register_style( 'global-styles', false, array(), true, true );
-			wp_add_inline_style( 'global-styles', $stylesheet );
-			wp_enqueue_style( 'global-styles' );
-		}
+	if ( isset( wp_styles()->registered['global-styles'] ) ) {
+		// There's a GS stylesheet (theme has theme.json), so we overwrite it.
+		wp_styles()->registered['global-styles']->extra['after'][0] = $stylesheet;
+	} else {
+		// No GS stylesheet (theme has no theme.json), so we enqueue a new one.
+		wp_register_style( 'global-styles', false, array(), true, true );
+		wp_add_inline_style( 'global-styles', $stylesheet );
+		wp_enqueue_style( 'global-styles' );
 	}
-	add_action( 'wp_enqueue_scripts', 'gutenberg_enqueue_global_styles_assets' );
 }
+add_action( 'wp_enqueue_scripts', 'gutenberg_enqueue_global_styles_assets' );
+add_action( 'wp_footer', 'gutenberg_enqueue_global_styles_assets' );