diff --git a/CHANGELOG.md b/CHANGELOG.md
index e152fa14b0..7ee58ded12 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,6 +6,9 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.
 
 ## [Unreleased]
 ### Added
+- Enabled authenticators can now be configured via a configuration file, or the
+  CONJUR_AUTHENTICATORS environment variable.
+  [cyberark/conjur##2173](https://github.com/cyberark/conjur/issues/#2173)
 - Trusted Proxies can now be configured with a configuration file or by setting
   the CONJUR_TRUSTED_PROXIES environment variable.
   [cyberark/conjur#2168](https://github.com/cyberark/conjur/issues/2168)
diff --git a/Gemfile b/Gemfile
index d8f3ae789e..9e4b1f95fc 100644
--- a/Gemfile
+++ b/Gemfile
@@ -87,7 +87,10 @@ gem 'websocket'
 gem 'jwt'
 gem 'openid_connect'
 
-gem "anyway_config", "~> 2.0"
+# Unpin version once this Github issue,
+# https://github.com/palkan/anyway_config/issues/82
+# is resolved
+gem "anyway_config", "2.1.0"
 
 group :development, :test do
   gem 'aruba'
diff --git a/Gemfile.lock b/Gemfile.lock
index 493dcd454e..171e656627 100644
--- a/Gemfile.lock
+++ b/Gemfile.lock
@@ -444,7 +444,7 @@ PLATFORMS
 
 DEPENDENCIES
   activesupport
-  anyway_config (~> 2.0)
+  anyway_config (= 2.1.0)
   aruba
   aws-sdk-iam
   base32-crockford
diff --git a/app/controllers/authenticate_controller.rb b/app/controllers/authenticate_controller.rb
index 4809abc3ec..fd680b4f47 100644
--- a/app/controllers/authenticate_controller.rb
+++ b/app/controllers/authenticate_controller.rb
@@ -23,7 +23,7 @@ def index
   def status
     Authentication::ValidateStatus.new.(
       authenticator_status_input: status_input,
-      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str(ENV)
+      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str
     )
     render(json: { status: "ok" })
   rescue => e
@@ -70,7 +70,7 @@ def authenticate(input = authenticator_input)
     authn_token = Authentication::Authenticate.new.(
       authenticator_input: input,
       authenticators: installed_authenticators,
-      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str(ENV)
+      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str
     )
     content_type = :json
     if encoded_response?
@@ -237,7 +237,7 @@ def installed_authenticators
   end
 
   def enabled_authenticators
-    Authentication::InstalledAuthenticators.enabled_authenticators(ENV)
+    Authentication::InstalledAuthenticators.enabled_authenticators
   end
 
   def encoded_response?
diff --git a/app/controllers/concerns/basic_authenticator.rb b/app/controllers/concerns/basic_authenticator.rb
index 6584316cb2..1506bce169 100644
--- a/app/controllers/concerns/basic_authenticator.rb
+++ b/app/controllers/concerns/basic_authenticator.rb
@@ -30,7 +30,7 @@ def authenticator_login(username, password)
     ::Authentication::Login.new.(
       authenticator_input: login_input(username, password),
       authenticators: installed_login_authenticators,
-      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str(ENV)
+      enabled_authenticators: Authentication::InstalledAuthenticators.enabled_authenticators_str
     )
   end
 
diff --git a/app/domain/authentication/authn_k8s/validate_pod_request.rb b/app/domain/authentication/authn_k8s/validate_pod_request.rb
index b6f70a03f7..f764cfdcc9 100644
--- a/app/domain/authentication/authn_k8s/validate_pod_request.rb
+++ b/app/domain/authentication/authn_k8s/validate_pod_request.rb
@@ -12,7 +12,7 @@ module AuthnK8s
         k8s_object_lookup_class: K8sObjectLookup,
         validate_webservice_is_whitelisted: Security::ValidateWebserviceIsWhitelisted.new,
         validate_role_can_access_webservice: Security::ValidateRoleCanAccessWebservice.new,
-        enabled_authenticators: InstalledAuthenticators.enabled_authenticators_str(ENV),
+        enabled_authenticators: InstalledAuthenticators.enabled_authenticators_str,
         validate_resource_restrictions: ResourceRestrictions::ValidateResourceRestrictions.new(
           extract_resource_restrictions:      ExtractK8sResourceRestrictions.new
         ),
diff --git a/app/domain/authentication/installed_authenticators.rb b/app/domain/authentication/installed_authenticators.rb
index 809aaf73aa..295d5c19de 100644
--- a/app/domain/authentication/installed_authenticators.rb
+++ b/app/domain/authentication/installed_authenticators.rb
@@ -33,21 +33,18 @@ def configured_authenticators
           .push(::Authentication::Common.default_authenticator_name)
       end
 
-      def enabled_authenticators(env)
+      def enabled_authenticators
         # Enabling via environment overrides enabling via CLI
-        env_enabled_authenticators(env) || db_enabled_authenticators
+        authenticators = 
+          Rails.application.config.conjur_config.authenticators
+        authenticators.empty? ? db_enabled_authenticators : authenticators
       end
 
-      def enabled_authenticators_str(env)
-        enabled_authenticators(env).join(',')
+      def enabled_authenticators_str
+        enabled_authenticators.join(',')
       end
 
       private
-
-      def env_enabled_authenticators(env)
-        authenticators = env["CONJUR_AUTHENTICATORS"]
-        authenticators.present? ? authenticators.split(',') : nil
-      end
       
       def db_enabled_authenticators
         # Always include 'authn' when enabling authenticators via CLI so that it
diff --git a/ci/docker-compose.yml b/ci/docker-compose.yml
index 4c9588cd7b..8a8efd6451 100644
--- a/ci/docker-compose.yml
+++ b/ci/docker-compose.yml
@@ -44,7 +44,7 @@ services:
       RAILS_ENV:
       REQUIRE_SIMPLECOV: "true"
       CONJUR_LOG_LEVEL: debug
-      CONJUR_AUTHENTICATORS: authn-ldap/test,authn-ldap/secure,authn-oidc/keycloak,authn-oidc,authn-config/env,authn-azure/prod,authn-gcp
+      CONJUR_AUTHENTICATORS: authn-ldap/test,authn-ldap/secure,authn-oidc/keycloak,authn-oidc,authn-k8s/test,authn-azure/prod,authn-gcp
       LDAP_URI: ldap://ldap-server:389
       LDAP_BASE: dc=conjur,dc=net
       LDAP_FILTER: '(uid=%s)'
diff --git a/cucumber/authenticators_config/features/authn_config.feature b/cucumber/authenticators_config/features/authn_config.feature
index 9b6795afb5..27a3702bb1 100644
--- a/cucumber/authenticators_config/features/authn_config.feature
+++ b/cucumber/authenticators_config/features/authn_config.feature
@@ -5,15 +5,15 @@ Feature: Authenticator configuration
     And I have user "authn-updater"
     And I load a policy:
     """
-    # authn-config/env is enabled in CONJUR_AUTHENTICATORS and used in tests to
+    # authn-k8s/test is enabled in CONJUR_AUTHENTICATORS and used in tests to
     # ensure that the env value continues to be used even when DB config exists.
     - !policy
-      id: conjur/authn-config/env
+      id: conjur/authn-k8s/test
       body:
       - !webservice
 
     - !policy
-      id: conjur/authn-config/db
+      id: conjur/authn-k8s/db
       body:
       - !webservice
 
@@ -35,48 +35,48 @@ Feature: Authenticator configuration
         resource: !webservice
 
     - !grant
-      role: !group conjur/authn-config/db/viewers
+      role: !group conjur/authn-k8s/db/viewers
       member: !user authn-viewer
 
     - !grant
-      role: !group conjur/authn-config/db/updaters
+      role: !group conjur/authn-k8s/db/updaters
       member: !user authn-updater
     """
 
   Scenario: Authenticator is not configured in database
     When I am the super-user
     And I retrieve the list of authenticators
-    Then the enabled authenticators contains "authn-config/env"
+    Then the enabled authenticators contains "authn-k8s/test"
 
   Scenario: Authenticator is enabled in the environment and disabled in the database
     When I am the super-user
-    And I successfully PATCH "/authn-config/env/cucumber" with body:
+    And I successfully PATCH "/authn-k8s/test/cucumber" with body:
     """
     enabled=false
     """
     And I retrieve the list of authenticators
-    Then the enabled authenticators contains "authn-config/env"
+    Then the enabled authenticators contains "authn-k8s/test"
 
   Scenario: Authenticator is successfully configured
     When I login as "authn-updater"
-    And I successfully PATCH "/authn-config/db/cucumber" with body:
+    And I successfully PATCH "/authn-k8s/db/cucumber" with body:
     """
     enabled=true
     """
     Then the HTTP response status code is 204
-    And authenticator "cucumber:webservice:conjur/authn-config/db" is enabled
+    And authenticator "cucumber:webservice:conjur/authn-k8s/db" is enabled
 
-    When I successfully PATCH "/authn-config/db/cucumber" with body:
+    When I successfully PATCH "/authn-k8s/db/cucumber" with body:
     """
     enabled=false
     """
     Then the HTTP response status code is 204
-    And authenticator "cucumber:webservice:conjur/authn-config/db" is disabled
+    And authenticator "cucumber:webservice:conjur/authn-k8s/db" is disabled
 
   Scenario: Authenticator account does not exist
     When I am the super-user
     And I save my place in the log file
-    And I PATCH "/authn-config/db/nope" with body:
+    And I PATCH "/authn-k8s/db/nope" with body:
     """
     enabled=true
     """
@@ -89,7 +89,7 @@ Feature: Authenticator configuration
   Scenario: Authenticator webservice does not exist
     When I am the super-user
     And I save my place in the log file
-    And I PATCH "/authn-config/db%2Fnope/cucumber" with body:
+    And I PATCH "/authn-k8s/db%2Fnope/cucumber" with body:
     """
     enabled=true
     """
@@ -102,12 +102,12 @@ Feature: Authenticator configuration
   Scenario: Authenticated user can not update authenticator
     When I login as "authn-viewer"
     And I save my place in the log file
-    And I PATCH "/authn-config/db/cucumber" with body:
+    And I PATCH "/authn-k8s/db/cucumber" with body:
     """
     enabled=true
     """
     Then the HTTP response status code is 403
-    And authenticator "cucumber:webservice:conjur/authn-config/db" is disabled
+    And authenticator "cucumber:webservice:conjur/authn-k8s/db" is disabled
     And The following appears in the log after my savepoint:
     """
     Errors::Authentication::Security::RoleNotAuthorizedOnResource
@@ -116,7 +116,7 @@ Feature: Authenticator configuration
   Scenario: Nested webservice can not be configured
     When I am the super-user
     And I save my place in the log file
-    And I PATCH "/authn-config/db%2Fstatus/cucumber" with body:
+    And I PATCH "/authn-k8s/db%2Fstatus/cucumber" with body:
     """
     enabled=true
     """
diff --git a/dev/start b/dev/start
index c69eadc354..626cd7f2b8 100755
--- a/dev/start
+++ b/dev/start
@@ -136,7 +136,7 @@ docker-compose exec -d conjur conjurctl server
 echo 'Checking if Conjur server is ready'
 conjur_isready?
 
-default_authenticators="authn,authn-config/env"
+default_authenticators="authn,authn-k8s/test"
 enabled_authenticators="$default_authenticators"
 
 env_args=
diff --git a/lib/conjur/conjur_config.rb b/lib/conjur/conjur_config.rb
index 1e6f0c3d61..e256436b2c 100644
--- a/lib/conjur/conjur_config.rb
+++ b/lib/conjur/conjur_config.rb
@@ -16,7 +16,8 @@ class ConjurConfig < Anyway::Config
 
     attr_config(
       # Read TRUSTED_PROXIES before default to maintain backwards compatibility
-      trusted_proxies: (ENV['TRUSTED_PROXIES'] || [])
+      trusted_proxies: (ENV['TRUSTED_PROXIES'] || []),
+      authenticators: []
     )
 
     # Perform validations and collect errors then report results as exception
@@ -24,6 +25,7 @@ class ConjurConfig < Anyway::Config
       invalid = []
 
       invalid << "trusted_proxies" unless trusted_proxies_valid?
+      invalid << "authenticators" unless authenticators_valid?
 
       unless invalid.empty?
         raise Errors::Conjur::InvalidConfigValues, invalid.join(', ')
@@ -32,7 +34,7 @@ class ConjurConfig < Anyway::Config
 
     # Get attribute sources without including attribute values
     def attribute_sources
-      to_source_trace.map { |k,v| [ k.to_sym, v[:source][:type] ] }.to_h
+      to_source_trace.map { |k, v| [ k.to_sym, v[:source][:type] ] }.to_h
     end
 
     # The Anyway config gem automatically converts a comma-separated env var to
@@ -43,9 +45,15 @@ def attribute_sources
     #
     # To address this inconsistent behavior, we use custom setters to ensure
     # list attributes are always converted to a a list type.
+    # We filed an issue regarding this behavior:
+    #   https://github.com/palkan/anyway_config/issues/82
 
     def trusted_proxies=(val)
-      super(str_to_list(val))
+      super(str_to_list(val)&.uniq)
+    end
+
+    def authenticators=(val)
+      super(str_to_list(val)&.uniq)
     end
 
     private
@@ -63,5 +71,18 @@ def trusted_proxies_valid?
     rescue
       false
     end
+
+    def authenticators_valid?
+      # TODO: Ideally we would check against the enabled authenticators
+      # in the DB. However, we need to figure out how to use code from the
+      # application without introducing warnings.
+      authenticators_regex = 
+        %r{^(authn|authn-(k8s|oidc|iam|ldap|gcp|azure)(/.+)?)$}
+      authenticators.all? do |authenticator|
+        authenticators_regex.match?(authenticator.strip)
+      end
+    rescue
+      false
+    end
   end
 end
diff --git a/spec/lib/conjur/conjur_config_spec.rb b/spec/lib/conjur/conjur_config_spec.rb
index a0a86a58b6..d535d7ffbb 100644
--- a/spec/lib/conjur/conjur_config_spec.rb
+++ b/spec/lib/conjur/conjur_config_spec.rb
@@ -86,23 +86,45 @@
   end
 
   describe "validation" do
-    let(:invalid_config) {
+    let(:invalid_trusted_proxies_config) {
       Conjur::ConjurConfig.new(trusted_proxies: "boop")
     }
 
+    let(:invalid_authenticators_config) {
+      Conjur::ConjurConfig.new(authenticators: "invalid-authn")
+    }
+
+    let(:invalid_config) {
+      Conjur::ConjurConfig.new(
+        authenticators: "invalid-authn", trusted_proxies: "boop"
+      )
+    }
+
     it "raises error when validation fails" do
+      expect { invalid_trusted_proxies_config }.
+        to raise_error(Errors::Conjur::InvalidConfigValues)
+      expect { invalid_authenticators_config }.
+        to raise_error(Errors::Conjur::InvalidConfigValues)
       expect { invalid_config }.
         to raise_error(Errors::Conjur::InvalidConfigValues)
     end
 
     it "includes the attribute that failed validation" do
-      expect { invalid_config }.
+      expect { invalid_trusted_proxies_config }.
         to raise_error(/trusted_proxies/)
+      expect { invalid_authenticators_config }.
+        to raise_error(/authenticators/)
+      expect { invalid_config }.
+        to raise_error(/trusted_proxies, authenticators/)
     end
 
     it "does not include the value that failed validation" do
-      expect { invalid_config }.
+      expect { invalid_trusted_proxies_config }.
         to_not raise_error(/boop/)
+      expect { invalid_authenticators_config }.
+        to_not raise_error(/invalid-authn/)
+      expect { invalid_config }.
+        to_not raise_error(/boop.*invalid-authn/)
     end
   end