diff --git a/cell_type_annotation.md b/cell_type_annotation.md index 5f8d986..d99b784 100644 --- a/cell_type_annotation.md +++ b/cell_type_annotation.md @@ -855,897 +855,7 @@ par(mfrow = c(3,3)) AUCell_exploreThresholds(cell.aucs[10:18], plotHist = TRUE, assign = TRUE) ``` - - -``` output -$`Haematoendothelial progenitors` -$`Haematoendothelial progenitors`$aucThr -$`Haematoendothelial progenitors`$aucThr$selected - R_k3 -0.273 - -$`Haematoendothelial progenitors`$aucThr$thresholds - threshold nCells -Global_k1 0.173 196 -L_k2 0.199 138 -R_k3 0.273 48 - -$`Haematoendothelial progenitors`$aucThr$comment -[1] "The global distribution overlaps the partial distributions. " - - -$`Haematoendothelial progenitors`$assignment - [1] "cell_9963" "cell_11842" "cell_9934" "cell_11154" "cell_10322" - [6] "cell_11337" "cell_11770" "cell_12021" "cell_11667" "cell_10047" -[11] "cell_11290" "cell_11733" "cell_11827" "cell_11404" "cell_11511" -[16] "cell_10146" "cell_10718" "cell_12090" "cell_10715" "cell_10458" -[21] "cell_10216" "cell_10010" "cell_11760" "cell_10126" "cell_10654" -[26] "cell_9813" "cell_11916" "cell_12082" "cell_11769" "cell_11484" -[31] "cell_11414" "cell_11932" "cell_11791" "cell_9974" "cell_11199" -[36] "cell_10256" "cell_10319" "cell_10977" "cell_10945" "cell_11077" -[41] "cell_10895" "cell_10168" "cell_9770" "cell_11772" "cell_11771" -[46] "cell_11611" "cell_11287" "cell_10095" - - -$`Intermediate mesoderm` -$`Intermediate mesoderm`$aucThr -$`Intermediate mesoderm`$aucThr$selected -minimumDens - 0.0723 - -$`Intermediate mesoderm`$aucThr$thresholds - threshold nCells -outlierOfGlobal 0.4388 0 -Global_k1 0.1984 262 -L_k2 0.2203 168 -R_k3 0.1547 516 -minimumDens 0.0723 841 - -$`Intermediate mesoderm`$aucThr$comment -[1] "The AUC might follow a normal distribution (random gene-set?). The global distribution overlaps the partial distributions. " - - -$`Intermediate mesoderm`$assignment - [1] "cell_11995" "cell_9963" "cell_10910" "cell_11021" "cell_10433" - [6] "cell_11395" "cell_10779" "cell_10883" "cell_10721" "cell_10116" - [11] "cell_10785" "cell_11781" "cell_11147" "cell_11842" "cell_11218" - [16] "cell_11441" "cell_11558" "cell_9979" "cell_10323" "cell_11663" - [21] "cell_10935" "cell_11567" "cell_10673" "cell_11634" "cell_10815" - [26] "cell_10077" "cell_9934" "cell_11888" "cell_10356" "cell_10490" - [31] "cell_9921" "cell_10062" "cell_9809" "cell_11906" "cell_10951" - [36] "cell_10520" "cell_10142" "cell_12176" "cell_11850" "cell_10284" - [41] "cell_9781" "cell_11885" "cell_11687" "cell_10523" "cell_11100" - [46] "cell_10881" "cell_10774" "cell_12107" "cell_11216" "cell_10439" - [51] "cell_10902" "cell_10856" "cell_11702" "cell_11269" "cell_10248" - [56] "cell_11883" "cell_11682" "cell_10877" "cell_9933" "cell_10843" - [61] "cell_11154" "cell_12052" "cell_9895" "cell_12028" "cell_10454" - [66] "cell_11750" "cell_12059" "cell_10615" "cell_10751" "cell_10387" - [71] "cell_10245" "cell_11767" "cell_9919" "cell_11976" "cell_11971" - [76] "cell_10797" "cell_10094" "cell_11724" "cell_11030" "cell_11855" - [81] "cell_10754" "cell_9905" "cell_11530" "cell_11352" "cell_10357" - [86] "cell_12074" "cell_12012" "cell_11561" "cell_11398" "cell_12075" - [91] "cell_12177" "cell_11653" "cell_10644" "cell_11993" "cell_10322" - [96] "cell_11900" "cell_11315" "cell_10474" "cell_10480" "cell_11220" -[101] "cell_10440" "cell_11596" "cell_10296" "cell_11337" "cell_11856" -[106] "cell_11728" "cell_10241" "cell_12016" "cell_11770" "cell_10917" -[111] "cell_11805" "cell_12002" "cell_10599" "cell_10846" "cell_10687" -[116] "cell_11330" "cell_11081" "cell_9953" "cell_10181" "cell_10395" -[121] "cell_12021" "cell_11667" "cell_10547" "cell_11329" "cell_10332" -[126] "cell_10562" "cell_11183" "cell_11138" "cell_10385" "cell_10125" -[131] "cell_10047" "cell_10038" "cell_10386" "cell_11673" "cell_10105" -[136] "cell_12162" "cell_10749" "cell_11549" "cell_11458" "cell_11290" -[141] "cell_11213" "cell_10849" "cell_11465" "cell_9885" "cell_10416" -[146] "cell_10847" "cell_11009" "cell_11733" "cell_10756" "cell_10479" -[151] "cell_11260" "cell_11002" "cell_10117" "cell_10933" "cell_11840" -[156] "cell_10922" "cell_11495" "cell_11380" "cell_10478" "cell_11026" -[161] "cell_11880" "cell_11896" "cell_12110" "cell_11115" "cell_10559" -[166] "cell_12151" "cell_11109" "cell_11014" "cell_11526" "cell_11018" -[171] "cell_11582" "cell_11476" "cell_10065" "cell_10628" "cell_11603" -[176] "cell_10825" "cell_9851" "cell_10634" "cell_10791" "cell_9844" -[181] "cell_10886" "cell_11931" "cell_11975" "cell_11150" "cell_10450" -[186] "cell_12115" "cell_11506" "cell_11239" "cell_11130" "cell_10011" -[191] "cell_11922" "cell_11827" "cell_10393" "cell_11982" "cell_10229" -[196] "cell_12140" "cell_9799" "cell_10907" "cell_11957" "cell_9784" -[201] "cell_11204" "cell_10292" "cell_11229" "cell_10692" "cell_11370" -[206] "cell_9972" "cell_10914" "cell_10027" "cell_10262" "cell_11657" -[211] "cell_11552" "cell_11123" "cell_10900" "cell_11093" "cell_9778" -[216] "cell_9938" "cell_11001" "cell_11619" "cell_12160" "cell_9876" -[221] "cell_9776" "cell_10394" "cell_10029" "cell_11377" "cell_11679" -[226] "cell_11054" "cell_11528" "cell_11913" "cell_11515" "cell_10435" -[231] "cell_10758" "cell_10220" "cell_10624" "cell_11716" "cell_11371" -[236] "cell_12042" "cell_10414" "cell_10828" "cell_10659" "cell_11424" -[241] "cell_11096" "cell_12171" "cell_11803" "cell_11404" "cell_10561" -[246] "cell_11180" "cell_10046" "cell_10009" "cell_9792" "cell_11853" -[251] "cell_11095" "cell_11511" "cell_11835" "cell_11463" "cell_10146" -[256] "cell_10317" "cell_11407" "cell_12173" "cell_10161" "cell_10438" -[261] "cell_10981" "cell_10079" "cell_11210" "cell_11174" "cell_10876" -[266] "cell_10087" "cell_11630" "cell_11434" "cell_9888" "cell_10367" -[271] "cell_11107" "cell_11994" "cell_10192" "cell_11822" "cell_10647" -[276] "cell_10436" "cell_11592" "cell_9817" "cell_11268" "cell_9961" -[281] "cell_10071" "cell_9959" "cell_11419" "cell_10585" "cell_11214" -[286] "cell_11084" "cell_11165" "cell_11327" "cell_11392" "cell_10718" -[291] "cell_10466" "cell_12090" "cell_11555" "cell_10995" "cell_10526" -[296] "cell_10761" "cell_11038" "cell_11232" "cell_10715" "cell_10458" -[301] "cell_10019" "cell_10328" "cell_10411" "cell_10313" "cell_9930" -[306] "cell_11114" "cell_9936" "cell_11234" "cell_10556" "cell_9846" -[311] "cell_11871" "cell_11285" "cell_10991" "cell_11939" "cell_10213" -[316] "cell_9863" "cell_11710" "cell_11256" "cell_10147" "cell_9989" -[321] "cell_10464" "cell_10953" "cell_10388" "cell_10216" "cell_10010" -[326] "cell_11485" "cell_10695" "cell_11760" "cell_11328" "cell_11620" -[331] "cell_10694" "cell_10175" "cell_9997" "cell_12147" "cell_10553" -[336] "cell_11266" "cell_10467" "cell_11233" "cell_10963" "cell_12149" -[341] "cell_9986" "cell_11276" "cell_11904" "cell_12179" "cell_9847" -[346] "cell_11303" "cell_11299" "cell_10956" "cell_10347" "cell_11599" -[351] "cell_10697" "cell_10190" "cell_10261" "cell_10498" "cell_11588" -[356] "cell_9977" "cell_11839" "cell_11777" "cell_11941" "cell_10645" -[361] "cell_10126" "cell_10833" "cell_10672" "cell_10654" "cell_11024" -[366] "cell_10164" "cell_11522" "cell_10407" "cell_12134" "cell_11056" -[371] "cell_11489" "cell_9969" "cell_9820" "cell_11017" "cell_9835" -[376] "cell_12064" "cell_11562" "cell_10345" "cell_10225" "cell_10783" -[381] "cell_12036" "cell_11333" "cell_10812" "cell_10998" "cell_9892" -[386] "cell_10360" "cell_12152" "cell_10508" "cell_9813" "cell_11124" -[391] "cell_11073" "cell_9859" "cell_10421" "cell_10930" "cell_10374" -[396] "cell_12097" "cell_11217" "cell_10800" "cell_11063" "cell_10363" -[401] "cell_10515" "cell_10267" "cell_11047" "cell_11744" "cell_11564" -[406] "cell_9849" "cell_11916" "cell_12082" "cell_12057" "cell_10664" -[411] "cell_11680" "cell_11542" "cell_10765" "cell_12066" "cell_11438" -[416] "cell_12072" "cell_10441" "cell_10396" "cell_10838" "cell_9832" -[421] "cell_10299" "cell_11769" "cell_11402" "cell_11736" "cell_11012" -[426] "cell_11484" "cell_11962" "cell_10604" "cell_11966" "cell_10370" -[431] "cell_10099" "cell_10669" "cell_11774" "cell_11579" "cell_10059" -[436] "cell_10511" "cell_10597" "cell_12031" "cell_10961" "cell_10497" -[441] "cell_11934" "cell_11353" "cell_11133" "cell_9837" "cell_11112" -[446] "cell_10272" "cell_10868" "cell_11734" "cell_10554" "cell_11683" -[451] "cell_10898" "cell_10623" "cell_10864" "cell_10217" "cell_10739" -[456] "cell_12174" "cell_12053" "cell_11859" "cell_10103" "cell_12167" -[461] "cell_10848" "cell_9975" "cell_10204" "cell_11678" "cell_10769" -[466] "cell_10369" "cell_10155" "cell_10860" "cell_10512" "cell_10333" -[471] "cell_11149" "cell_10560" "cell_10510" "cell_11658" "cell_10354" -[476] "cell_11141" "cell_11263" "cell_12058" "cell_9856" "cell_11208" -[481] "cell_9779" "cell_11598" "cell_10231" "cell_11547" "cell_10468" -[486] "cell_11961" "cell_10610" "cell_10453" "cell_10048" "cell_11304" -[491] "cell_10571" "cell_11778" "cell_10546" "cell_12111" "cell_11120" -[496] "cell_9940" "cell_12040" "cell_11471" "cell_11470" "cell_11321" -[501] "cell_10236" "cell_11176" "cell_12094" "cell_10509" "cell_10133" -[506] "cell_10359" "cell_11595" "cell_11019" "cell_10602" "cell_11414" -[511] "cell_11838" "cell_11932" "cell_11057" "cell_10303" "cell_10750" -[516] "cell_9880" "cell_10224" "cell_10885" "cell_11281" "cell_10399" -[521] "cell_9816" "cell_10737" "cell_10250" "cell_11040" "cell_9873" -[526] "cell_11782" "cell_10227" "cell_12125" "cell_11714" "cell_11104" -[531] "cell_11354" "cell_10660" "cell_9907" "cell_10249" "cell_10611" -[536] "cell_10018" "cell_11389" "cell_10098" "cell_10401" "cell_11418" -[541] "cell_12092" "cell_10890" "cell_10850" "cell_11791" "cell_11988" -[546] "cell_11897" "cell_10026" "cell_10403" "cell_10030" "cell_11688" -[551] "cell_11571" "cell_10696" "cell_9974" "cell_9967" "cell_11381" -[556] "cell_11972" "cell_12143" "cell_11837" "cell_10521" "cell_11280" -[561] "cell_11694" "cell_10166" "cell_11010" "cell_10947" "cell_11901" -[566] "cell_12044" "cell_10586" "cell_10428" "cell_10115" "cell_11357" -[571] "cell_11121" "cell_12114" "cell_12076" "cell_9823" "cell_11162" -[576] "cell_10836" "cell_11954" "cell_11713" "cell_11168" "cell_10700" -[581] "cell_11845" "cell_11557" "cell_11346" "cell_9962" "cell_11386" -[586] "cell_11799" "cell_10869" "cell_11411" "cell_10766" "cell_11450" -[591] "cell_11058" "cell_10481" "cell_10123" "cell_10238" "cell_10107" -[596] "cell_9968" "cell_11951" "cell_11811" "cell_11519" "cell_11172" -[601] "cell_10661" "cell_10534" "cell_10904" "cell_12081" "cell_10237" -[606] "cell_10247" "cell_10380" "cell_11199" "cell_11447" "cell_11854" -[611] "cell_11524" "cell_10058" "cell_11586" "cell_10234" "cell_11743" -[616] "cell_10256" "cell_11775" "cell_11270" "cell_10089" "cell_9777" -[621] "cell_10635" "cell_10049" "cell_10410" "cell_10675" "cell_10476" -[626] "cell_10832" "cell_10940" "cell_10788" "cell_11818" "cell_11502" -[631] "cell_10731" "cell_12166" "cell_10646" "cell_11397" "cell_10151" -[636] "cell_12085" "cell_11435" "cell_10239" "cell_11715" "cell_12060" -[641] "cell_11798" "cell_9929" "cell_10189" "cell_10212" "cell_9797" -[646] "cell_11529" "cell_11033" "cell_11794" "cell_9812" "cell_9992" -[651] "cell_11696" "cell_10680" "cell_10319" "cell_10120" "cell_10601" -[656] "cell_12145" "cell_10451" "cell_11243" "cell_9824" "cell_11070" -[661] "cell_11983" "cell_11986" "cell_10422" "cell_11664" "cell_10143" -[666] "cell_11368" "cell_11580" "cell_11583" "cell_11312" "cell_11862" -[671] "cell_11493" "cell_11226" "cell_9785" "cell_10417" "cell_9931" -[676] "cell_11277" "cell_11261" "cell_11642" "cell_10977" "cell_10424" -[681] "cell_10588" "cell_9803" "cell_11938" "cell_11877" "cell_10271" -[686] "cell_10177" "cell_10437" "cell_10741" "cell_10580" "cell_11102" -[691] "cell_10945" "cell_11077" "cell_9916" "cell_9861" "cell_10037" -[696] "cell_10485" "cell_10443" "cell_10140" "cell_10285" "cell_11618" -[701] "cell_9845" "cell_11469" "cell_11140" "cell_10452" "cell_10352" -[706] "cell_9894" "cell_11823" "cell_10944" "cell_10488" "cell_10867" -[711] "cell_11516" "cell_12116" "cell_10755" "cell_10072" "cell_11393" -[716] "cell_11650" "cell_10878" "cell_11400" "cell_9868" "cell_10817" -[721] "cell_11700" "cell_11085" "cell_12007" "cell_11998" "cell_12158" -[726] "cell_10315" "cell_10698" "cell_10471" "cell_10111" "cell_11945" -[731] "cell_10999" "cell_11225" "cell_11083" "cell_11539" "cell_12113" -[736] "cell_11158" "cell_9802" "cell_11173" "cell_10034" "cell_10193" -[741] "cell_12083" "cell_11296" "cell_11007" "cell_10895" "cell_10725" -[746] "cell_11709" "cell_10626" "cell_10168" "cell_9770" "cell_10870" -[751] "cell_10950" "cell_9924" "cell_12006" "cell_9983" "cell_10787" -[756] "cell_10054" "cell_11118" "cell_10732" "cell_10198" "cell_11923" -[761] "cell_10518" "cell_10418" "cell_10484" "cell_10522" "cell_10153" -[766] "cell_11483" "cell_11309" "cell_11445" "cell_11754" "cell_11914" -[771] "cell_10677" "cell_11772" "cell_11697" "cell_9942" "cell_10202" -[776] "cell_10383" "cell_11074" "cell_11212" "cell_10572" "cell_11342" -[781] "cell_10593" "cell_10283" "cell_12056" "cell_9819" "cell_11771" -[786] "cell_11591" "cell_9998" "cell_11144" "cell_11015" "cell_10381" -[791] "cell_10937" "cell_11265" "cell_10086" "cell_10187" "cell_10215" -[796] "cell_11611" "cell_9911" "cell_11308" "cell_10735" "cell_11978" -[801] "cell_11067" "cell_10491" "cell_11003" "cell_10989" "cell_11480" -[806] "cell_10255" "cell_10312" "cell_11548" "cell_11207" "cell_9920" -[811] "cell_10209" "cell_11211" "cell_11422" "cell_11360" "cell_10305" -[816] "cell_10821" "cell_10461" "cell_10008" "cell_10801" "cell_11319" -[821] "cell_10078" "cell_11651" "cell_11287" "cell_10389" "cell_10135" -[826] "cell_10773" "cell_11246" "cell_11623" "cell_11627" "cell_11031" -[831] "cell_10200" "cell_11113" "cell_9794" "cell_12023" "cell_11197" -[836] "cell_11313" "cell_11129" "cell_11597" "cell_9807" "cell_10095" -[841] "cell_11860" - - -$Mesenchyme -$Mesenchyme$aucThr -$Mesenchyme$aucThr$selected -minimumDens - 0.418 - -$Mesenchyme$aucThr$thresholds - threshold nCells -Global_k1 0.261 168 -L_k2 0.374 95 -R_k3 0.171 346 -minimumDens 0.418 81 - -$Mesenchyme$aucThr$comment -[1] "" - - -$Mesenchyme$assignment - [1] "cell_10062" "cell_11100" "cell_12059" "cell_10387" "cell_9919" - [6] "cell_10754" "cell_12075" "cell_10474" "cell_10480" "cell_11081" -[11] "cell_10125" "cell_12162" "cell_11465" "cell_11840" "cell_9784" -[16] "cell_11204" "cell_10900" "cell_9938" "cell_10394" "cell_11377" -[21] "cell_11407" "cell_10367" "cell_9961" "cell_9930" "cell_11710" -[26] "cell_10388" "cell_12147" "cell_10963" "cell_11276" "cell_10498" -[31] "cell_10645" "cell_10164" "cell_11489" "cell_9969" "cell_12064" -[36] "cell_10363" "cell_12072" "cell_10669" "cell_10511" "cell_11934" -[41] "cell_10864" "cell_10204" "cell_10155" "cell_11208" "cell_11120" -[46] "cell_9880" "cell_10250" "cell_12092" "cell_11897" "cell_11571" -[51] "cell_11837" "cell_11010" "cell_10947" "cell_12044" "cell_11954" -[56] "cell_9962" "cell_10237" "cell_11502" "cell_10151" "cell_11435" -[61] "cell_12060" "cell_10451" "cell_11243" "cell_11070" "cell_11983" -[66] "cell_11580" "cell_11583" "cell_9785" "cell_10177" "cell_10485" -[71] "cell_11469" "cell_12113" "cell_10677" "cell_10383" "cell_11015" -[76] "cell_10491" "cell_11480" "cell_10461" "cell_10389" "cell_9794" -[81] "cell_9807" - - -$`Neural crest` -$`Neural crest`$aucThr -$`Neural crest`$aucThr$selected -outlierOfGlobal - 0.331 - -$`Neural crest`$aucThr$thresholds - threshold nCells -outlierOfGlobal 0.331 0 -Global_k1 0.181 264 -L_k2 0.168 373 -R_k3 0.254 12 - -$`Neural crest`$aucThr$comment -[1] "The AUC might follow a normal distribution (random gene-set?). The right distribution is taller. " - - -$`Neural crest`$assignment -character(0) - - -$NMP -$NMP$aucThr -$NMP$aucThr$selected - L_k2 -0.314 - -$NMP$aucThr$thresholds - threshold nCells -Global_k1 0.1873 195 -L_k2 0.3143 42 -R_k3 0.0961 677 - -$NMP$aucThr$comment -[1] "" - - -$NMP$assignment - [1] "cell_11021" "cell_10721" "cell_10116" "cell_10248" "cell_12052" - [6] "cell_10440" "cell_11380" "cell_9851" "cell_10886" "cell_11239" -[11] "cell_11180" "cell_10647" "cell_10411" "cell_11234" "cell_12149" -[16] "cell_9977" "cell_9820" "cell_12097" "cell_10597" "cell_10868" -[21] "cell_11149" "cell_10231" "cell_10453" "cell_11040" "cell_11104" -[26] "cell_10696" "cell_10380" "cell_10234" "cell_10832" "cell_9929" -[31] "cell_9824" "cell_11368" "cell_10140" "cell_11393" "cell_11998" -[36] "cell_10698" "cell_11083" "cell_12006" "cell_11207" "cell_10821" -[41] "cell_11319" "cell_11597" - - -$`Paraxial mesoderm` -$`Paraxial mesoderm`$aucThr -$`Paraxial mesoderm`$aucThr$selected -minimumDens - 0.0736 - -$`Paraxial mesoderm`$aucThr$thresholds - threshold nCells -Global_k1 0.1970 181 -L_k2 0.5372 0 -R_k3 0.0667 841 -minimumDens 0.0736 840 - -$`Paraxial mesoderm`$aucThr$comment -[1] "The global distribution overlaps the partial distributions. " - - -$`Paraxial mesoderm`$assignment - [1] "cell_11995" "cell_9963" "cell_10910" "cell_11021" "cell_10433" - [6] "cell_11395" "cell_10779" "cell_10883" "cell_10721" "cell_10116" - [11] "cell_10785" "cell_11781" "cell_11147" "cell_11842" "cell_11218" - [16] "cell_11441" "cell_11558" "cell_9979" "cell_10323" "cell_11663" - [21] "cell_10935" "cell_11567" "cell_10673" "cell_11634" "cell_10815" - [26] "cell_10077" "cell_9934" "cell_11888" "cell_10356" "cell_10490" - [31] "cell_9921" "cell_10062" "cell_9809" "cell_11906" "cell_10951" - [36] "cell_10520" "cell_12176" "cell_11850" "cell_10284" "cell_9781" - [41] "cell_11885" "cell_11687" "cell_10523" "cell_11100" "cell_10881" - [46] "cell_10774" "cell_12107" "cell_11216" "cell_10439" "cell_10902" - [51] "cell_10856" "cell_11702" "cell_11269" "cell_10248" "cell_11883" - [56] "cell_11682" "cell_10877" "cell_9933" "cell_10843" "cell_11154" - [61] "cell_12052" "cell_9895" "cell_12028" "cell_10454" "cell_11750" - [66] "cell_12059" "cell_10615" "cell_10751" "cell_10387" "cell_10245" - [71] "cell_11767" "cell_9919" "cell_11976" "cell_11971" "cell_10797" - [76] "cell_10094" "cell_11724" "cell_11030" "cell_11855" "cell_10754" - [81] "cell_9905" "cell_11530" "cell_11352" "cell_10357" "cell_12074" - [86] "cell_12012" "cell_11561" "cell_11398" "cell_12075" "cell_12177" - [91] "cell_11653" "cell_10644" "cell_11993" "cell_10322" "cell_11900" - [96] "cell_11315" "cell_10474" "cell_10480" "cell_11220" "cell_10440" -[101] "cell_11596" "cell_10296" "cell_11337" "cell_11856" "cell_11728" -[106] "cell_10241" "cell_12016" "cell_11770" "cell_10917" "cell_11805" -[111] "cell_12002" "cell_10599" "cell_10846" "cell_10687" "cell_11330" -[116] "cell_11081" "cell_9953" "cell_10181" "cell_10395" "cell_12021" -[121] "cell_11667" "cell_10547" "cell_11329" "cell_10332" "cell_10562" -[126] "cell_11183" "cell_11138" "cell_10385" "cell_10125" "cell_10047" -[131] "cell_10038" "cell_10386" "cell_11673" "cell_10105" "cell_12162" -[136] "cell_10749" "cell_11549" "cell_11458" "cell_11290" "cell_11213" -[141] "cell_10849" "cell_11465" "cell_9885" "cell_10416" "cell_10847" -[146] "cell_11009" "cell_11733" "cell_10756" "cell_10479" "cell_11260" -[151] "cell_11002" "cell_10117" "cell_10933" "cell_11840" "cell_10922" -[156] "cell_11495" "cell_11380" "cell_10478" "cell_11026" "cell_11880" -[161] "cell_11896" "cell_12110" "cell_11115" "cell_10559" "cell_12151" -[166] "cell_11109" "cell_11014" "cell_11526" "cell_11018" "cell_11582" -[171] "cell_11476" "cell_10065" "cell_10628" "cell_11603" "cell_10825" -[176] "cell_9851" "cell_10634" "cell_10791" "cell_9844" "cell_10886" -[181] "cell_11931" "cell_11975" "cell_11150" "cell_10450" "cell_12115" -[186] "cell_11506" "cell_11239" "cell_11130" "cell_10011" "cell_11922" -[191] "cell_11827" "cell_10393" "cell_11982" "cell_10229" "cell_12140" -[196] "cell_9799" "cell_10907" "cell_11957" "cell_9784" "cell_11204" -[201] "cell_10292" "cell_11229" "cell_10692" "cell_11370" "cell_9972" -[206] "cell_10914" "cell_10027" "cell_10262" "cell_11657" "cell_11552" -[211] "cell_12029" "cell_11123" "cell_10900" "cell_11093" "cell_9778" -[216] "cell_9938" "cell_11001" "cell_11619" "cell_12160" "cell_9876" -[221] "cell_9776" "cell_10394" "cell_10029" "cell_11377" "cell_11679" -[226] "cell_11054" "cell_11528" "cell_11913" "cell_11515" "cell_10435" -[231] "cell_10758" "cell_10220" "cell_10624" "cell_11716" "cell_11371" -[236] "cell_12042" "cell_10414" "cell_10828" "cell_10659" "cell_11424" -[241] "cell_11096" "cell_12171" "cell_11803" "cell_11404" "cell_11180" -[246] "cell_10046" "cell_10009" "cell_9792" "cell_11853" "cell_11095" -[251] "cell_11511" "cell_11835" "cell_11463" "cell_10146" "cell_10317" -[256] "cell_11407" "cell_12173" "cell_10161" "cell_10438" "cell_10981" -[261] "cell_10079" "cell_11210" "cell_11174" "cell_10876" "cell_10087" -[266] "cell_11630" "cell_11434" "cell_9888" "cell_10367" "cell_11107" -[271] "cell_11994" "cell_10192" "cell_11822" "cell_10647" "cell_10436" -[276] "cell_11592" "cell_9817" "cell_11268" "cell_9961" "cell_10071" -[281] "cell_9959" "cell_11419" "cell_10585" "cell_11214" "cell_11084" -[286] "cell_11165" "cell_11327" "cell_11392" "cell_10718" "cell_10466" -[291] "cell_12090" "cell_11555" "cell_10995" "cell_10526" "cell_10761" -[296] "cell_11038" "cell_11232" "cell_10715" "cell_10458" "cell_10019" -[301] "cell_10328" "cell_10411" "cell_10313" "cell_9930" "cell_11114" -[306] "cell_9936" "cell_11234" "cell_10556" "cell_9846" "cell_11871" -[311] "cell_11285" "cell_10991" "cell_11939" "cell_10213" "cell_9863" -[316] "cell_11710" "cell_11256" "cell_10147" "cell_9989" "cell_10464" -[321] "cell_10953" "cell_10388" "cell_10216" "cell_10010" "cell_11485" -[326] "cell_10695" "cell_11760" "cell_11328" "cell_11620" "cell_10694" -[331] "cell_10175" "cell_9997" "cell_12147" "cell_10553" "cell_11266" -[336] "cell_10467" "cell_11233" "cell_10963" "cell_12149" "cell_9986" -[341] "cell_11276" "cell_11904" "cell_12179" "cell_9847" "cell_11303" -[346] "cell_11299" "cell_10956" "cell_10347" "cell_11599" "cell_10697" -[351] "cell_10190" "cell_10261" "cell_10498" "cell_11588" "cell_9977" -[356] "cell_11839" "cell_11777" "cell_11941" "cell_10645" "cell_10126" -[361] "cell_10833" "cell_10672" "cell_10654" "cell_11024" "cell_10164" -[366] "cell_11522" "cell_10407" "cell_12134" "cell_11056" "cell_11489" -[371] "cell_9969" "cell_9820" "cell_11017" "cell_9835" "cell_12064" -[376] "cell_11562" "cell_10345" "cell_10225" "cell_10783" "cell_12036" -[381] "cell_11333" "cell_10812" "cell_10998" "cell_9892" "cell_10360" -[386] "cell_12152" "cell_10508" "cell_9813" "cell_11124" "cell_11073" -[391] "cell_9859" "cell_10421" "cell_10930" "cell_10374" "cell_12097" -[396] "cell_11217" "cell_10800" "cell_11063" "cell_10363" "cell_10515" -[401] "cell_10267" "cell_11047" "cell_11744" "cell_11564" "cell_9849" -[406] "cell_11916" "cell_12082" "cell_12057" "cell_10664" "cell_11680" -[411] "cell_11542" "cell_10765" "cell_12066" "cell_11438" "cell_12072" -[416] "cell_10441" "cell_10396" "cell_10838" "cell_9832" "cell_10299" -[421] "cell_11769" "cell_11402" "cell_11736" "cell_11012" "cell_11484" -[426] "cell_11962" "cell_10604" "cell_11966" "cell_10370" "cell_10099" -[431] "cell_10669" "cell_11774" "cell_11579" "cell_10059" "cell_10511" -[436] "cell_10597" "cell_12031" "cell_10961" "cell_10497" "cell_11934" -[441] "cell_11353" "cell_11133" "cell_9837" "cell_11112" "cell_10272" -[446] "cell_10868" "cell_11734" "cell_10554" "cell_11683" "cell_10898" -[451] "cell_10623" "cell_10864" "cell_10217" "cell_10739" "cell_12174" -[456] "cell_12053" "cell_11859" "cell_10103" "cell_12167" "cell_10848" -[461] "cell_9975" "cell_10204" "cell_11678" "cell_10769" "cell_10369" -[466] "cell_10155" "cell_10860" "cell_10512" "cell_10333" "cell_11149" -[471] "cell_10560" "cell_10510" "cell_11658" "cell_10354" "cell_11141" -[476] "cell_11263" "cell_12058" "cell_9856" "cell_11208" "cell_9779" -[481] "cell_11598" "cell_10231" "cell_11547" "cell_10468" "cell_11961" -[486] "cell_10610" "cell_10453" "cell_10048" "cell_11304" "cell_10571" -[491] "cell_11778" "cell_10546" "cell_12111" "cell_11120" "cell_9940" -[496] "cell_12040" "cell_11471" "cell_11470" "cell_11321" "cell_10236" -[501] "cell_11176" "cell_12094" "cell_10509" "cell_10133" "cell_10359" -[506] "cell_11595" "cell_11019" "cell_10602" "cell_11414" "cell_11838" -[511] "cell_11932" "cell_11057" "cell_10303" "cell_10750" "cell_9880" -[516] "cell_10224" "cell_10885" "cell_11281" "cell_10399" "cell_9816" -[521] "cell_10737" "cell_10250" "cell_11040" "cell_9873" "cell_11782" -[526] "cell_10227" "cell_12125" "cell_11714" "cell_11104" "cell_11354" -[531] "cell_10660" "cell_9907" "cell_10249" "cell_10611" "cell_10018" -[536] "cell_11389" "cell_10098" "cell_10401" "cell_11418" "cell_12092" -[541] "cell_10890" "cell_10850" "cell_11791" "cell_11988" "cell_11897" -[546] "cell_10026" "cell_10403" "cell_10030" "cell_11688" "cell_11571" -[551] "cell_10696" "cell_9974" "cell_9967" "cell_11381" "cell_11972" -[556] "cell_12143" "cell_11837" "cell_10521" "cell_11280" "cell_11694" -[561] "cell_10166" "cell_11010" "cell_10947" "cell_11901" "cell_12044" -[566] "cell_10586" "cell_10428" "cell_10115" "cell_11357" "cell_11121" -[571] "cell_12114" "cell_12076" "cell_9823" "cell_11162" "cell_10836" -[576] "cell_11954" "cell_11713" "cell_11168" "cell_10700" "cell_11845" -[581] "cell_11557" "cell_11346" "cell_9962" "cell_11386" "cell_11799" -[586] "cell_10869" "cell_11411" "cell_10766" "cell_11450" "cell_11058" -[591] "cell_10481" "cell_10123" "cell_10238" "cell_10107" "cell_9968" -[596] "cell_11951" "cell_11811" "cell_11519" "cell_11172" "cell_10661" -[601] "cell_10534" "cell_10904" "cell_12081" "cell_10237" "cell_10247" -[606] "cell_10380" "cell_11199" "cell_11447" "cell_11854" "cell_11524" -[611] "cell_10058" "cell_11586" "cell_10234" "cell_11743" "cell_10256" -[616] "cell_11775" "cell_11270" "cell_10089" "cell_9777" "cell_10635" -[621] "cell_10049" "cell_10410" "cell_10675" "cell_10476" "cell_10832" -[626] "cell_10940" "cell_10788" "cell_11818" "cell_11502" "cell_10731" -[631] "cell_12166" "cell_10646" "cell_11397" "cell_10151" "cell_12085" -[636] "cell_11435" "cell_10239" "cell_11715" "cell_12060" "cell_11798" -[641] "cell_9929" "cell_10189" "cell_10212" "cell_9797" "cell_11529" -[646] "cell_11033" "cell_11794" "cell_9812" "cell_9992" "cell_11696" -[651] "cell_10680" "cell_10319" "cell_10120" "cell_10601" "cell_12145" -[656] "cell_10451" "cell_11243" "cell_9824" "cell_11070" "cell_11983" -[661] "cell_11986" "cell_10422" "cell_11664" "cell_10143" "cell_11368" -[666] "cell_11580" "cell_11583" "cell_11312" "cell_11862" "cell_11493" -[671] "cell_11226" "cell_9785" "cell_10417" "cell_9931" "cell_11277" -[676] "cell_11261" "cell_11642" "cell_10977" "cell_10424" "cell_10588" -[681] "cell_9803" "cell_11938" "cell_11877" "cell_10271" "cell_10177" -[686] "cell_10437" "cell_10741" "cell_10580" "cell_11102" "cell_10945" -[691] "cell_11077" "cell_9916" "cell_9861" "cell_10037" "cell_10485" -[696] "cell_10443" "cell_10140" "cell_10285" "cell_11618" "cell_9845" -[701] "cell_11469" "cell_11140" "cell_10452" "cell_10352" "cell_9894" -[706] "cell_11823" "cell_10944" "cell_10488" "cell_10867" "cell_11516" -[711] "cell_12116" "cell_10755" "cell_10072" "cell_11393" "cell_11650" -[716] "cell_10878" "cell_11400" "cell_9868" "cell_10817" "cell_11700" -[721] "cell_11085" "cell_12007" "cell_11998" "cell_12158" "cell_10315" -[726] "cell_10698" "cell_10471" "cell_10111" "cell_11945" "cell_10999" -[731] "cell_11225" "cell_11083" "cell_11539" "cell_12113" "cell_11158" -[736] "cell_9802" "cell_11173" "cell_10034" "cell_10193" "cell_12083" -[741] "cell_11296" "cell_11007" "cell_10895" "cell_10725" "cell_11709" -[746] "cell_10626" "cell_10168" "cell_9770" "cell_10870" "cell_10950" -[751] "cell_9924" "cell_12006" "cell_9983" "cell_10787" "cell_10054" -[756] "cell_11118" "cell_10732" "cell_10198" "cell_11923" "cell_10518" -[761] "cell_10418" "cell_10484" "cell_10522" "cell_10153" "cell_11483" -[766] "cell_11309" "cell_11445" "cell_11754" "cell_11914" "cell_10677" -[771] "cell_11772" "cell_11697" "cell_9942" "cell_10202" "cell_10383" -[776] "cell_11074" "cell_11212" "cell_10572" "cell_11342" "cell_10593" -[781] "cell_10283" "cell_12056" "cell_9819" "cell_11771" "cell_11591" -[786] "cell_9998" "cell_11144" "cell_11015" "cell_10381" "cell_10937" -[791] "cell_11265" "cell_10086" "cell_10187" "cell_10215" "cell_11611" -[796] "cell_9911" "cell_11308" "cell_10735" "cell_11978" "cell_11067" -[801] "cell_10491" "cell_11003" "cell_10989" "cell_11480" "cell_10255" -[806] "cell_10312" "cell_11548" "cell_11207" "cell_9920" "cell_10209" -[811] "cell_11211" "cell_11422" "cell_11360" "cell_10305" "cell_10821" -[816] "cell_10461" "cell_10008" "cell_10801" "cell_11319" "cell_10078" -[821] "cell_11651" "cell_11287" "cell_10389" "cell_10135" "cell_10773" -[826] "cell_11246" "cell_11623" "cell_11627" "cell_11031" "cell_10200" -[831] "cell_11113" "cell_9794" "cell_12023" "cell_11197" "cell_11313" -[836] "cell_11129" "cell_11597" "cell_9807" "cell_10095" "cell_11860" - - -$`Pharyngeal mesoderm` -$`Pharyngeal mesoderm`$aucThr -$`Pharyngeal mesoderm`$aucThr$selected -minimumDens - 0.0816 - -$`Pharyngeal mesoderm`$aucThr$thresholds - threshold nCells -Global_k1 0.2244 228 -L_k2 0.5680 0 -R_k3 0.0893 837 -minimumDens 0.0816 838 - -$`Pharyngeal mesoderm`$aucThr$comment -[1] "The global distribution overlaps the partial distributions. " - - -$`Pharyngeal mesoderm`$assignment - [1] "cell_11995" "cell_9963" "cell_10910" "cell_11021" "cell_10433" - [6] "cell_11395" "cell_10779" "cell_10883" "cell_10721" "cell_10116" - [11] "cell_10785" "cell_11781" "cell_11147" "cell_11842" "cell_11218" - [16] "cell_11441" "cell_11558" "cell_9979" "cell_10323" "cell_11663" - [21] "cell_10935" "cell_11567" "cell_10673" "cell_11634" "cell_10815" - [26] "cell_10077" "cell_9934" "cell_11888" "cell_10356" "cell_10490" - [31] "cell_9921" "cell_10062" "cell_9809" "cell_11906" "cell_10951" - [36] "cell_10520" "cell_12176" "cell_11850" "cell_10284" "cell_9781" - [41] "cell_11885" "cell_11687" "cell_10523" "cell_11100" "cell_10881" - [46] "cell_10774" "cell_12107" "cell_11216" "cell_10439" "cell_10902" - [51] "cell_10856" "cell_11702" "cell_11269" "cell_10248" "cell_11883" - [56] "cell_11682" "cell_10877" "cell_9933" "cell_10843" "cell_11154" - [61] "cell_12052" "cell_9895" "cell_12028" "cell_10454" "cell_11750" - [66] "cell_12059" "cell_10615" "cell_10751" "cell_10387" "cell_10245" - [71] "cell_11767" "cell_9919" "cell_11976" "cell_11971" "cell_10797" - [76] "cell_10094" "cell_11724" "cell_11030" "cell_11855" "cell_10754" - [81] "cell_9905" "cell_11530" "cell_11352" "cell_10357" "cell_12074" - [86] "cell_12012" "cell_11561" "cell_11398" "cell_12075" "cell_12177" - [91] "cell_11653" "cell_10644" "cell_11993" "cell_10322" "cell_11900" - [96] "cell_11315" "cell_10474" "cell_10480" "cell_11220" "cell_10440" -[101] "cell_11596" "cell_10296" "cell_11337" "cell_11856" "cell_11728" -[106] "cell_10241" "cell_12016" "cell_11770" "cell_10917" "cell_11805" -[111] "cell_12002" "cell_10599" "cell_10846" "cell_10687" "cell_11330" -[116] "cell_11081" "cell_9953" "cell_10181" "cell_10395" "cell_12021" -[121] "cell_11667" "cell_10547" "cell_11329" "cell_10332" "cell_10562" -[126] "cell_11183" "cell_11138" "cell_10385" "cell_10125" "cell_10047" -[131] "cell_10038" "cell_10386" "cell_11673" "cell_10105" "cell_12162" -[136] "cell_10749" "cell_11549" "cell_11458" "cell_11290" "cell_11213" -[141] "cell_10849" "cell_11465" "cell_9885" "cell_10416" "cell_10847" -[146] "cell_11009" "cell_11733" "cell_10756" "cell_10479" "cell_11260" -[151] "cell_11002" "cell_10117" "cell_10933" "cell_11840" "cell_10922" -[156] "cell_11495" "cell_11380" "cell_10478" "cell_11026" "cell_11880" -[161] "cell_11896" "cell_12110" "cell_11115" "cell_10559" "cell_12151" -[166] "cell_11109" "cell_11014" "cell_11526" "cell_11018" "cell_11582" -[171] "cell_11476" "cell_10065" "cell_10628" "cell_11603" "cell_10825" -[176] "cell_9851" "cell_10634" "cell_10791" "cell_9844" "cell_10886" -[181] "cell_11931" "cell_11975" "cell_11150" "cell_10450" "cell_12115" -[186] "cell_11506" "cell_11239" "cell_11130" "cell_10011" "cell_11922" -[191] "cell_11827" "cell_10393" "cell_11982" "cell_10229" "cell_12140" -[196] "cell_9799" "cell_10907" "cell_11957" "cell_9784" "cell_11204" -[201] "cell_10292" "cell_11229" "cell_10692" "cell_11370" "cell_9972" -[206] "cell_10914" "cell_10027" "cell_10262" "cell_11657" "cell_11552" -[211] "cell_11123" "cell_10900" "cell_11093" "cell_9778" "cell_9938" -[216] "cell_11001" "cell_11619" "cell_12160" "cell_9876" "cell_9776" -[221] "cell_10394" "cell_10029" "cell_11377" "cell_11679" "cell_11054" -[226] "cell_11528" "cell_11913" "cell_11515" "cell_10435" "cell_10758" -[231] "cell_10220" "cell_10624" "cell_11716" "cell_11371" "cell_12042" -[236] "cell_10414" "cell_10828" "cell_10659" "cell_11424" "cell_11096" -[241] "cell_12171" "cell_11803" "cell_11404" "cell_11180" "cell_10046" -[246] "cell_10009" "cell_9792" "cell_11853" "cell_11095" "cell_11511" -[251] "cell_11835" "cell_11463" "cell_10146" "cell_10317" "cell_11407" -[256] "cell_12173" "cell_10161" "cell_10438" "cell_10981" "cell_10079" -[261] "cell_11210" "cell_11174" "cell_10876" "cell_10087" "cell_11630" -[266] "cell_11434" "cell_9888" "cell_10367" "cell_11107" "cell_11994" -[271] "cell_10192" "cell_11822" "cell_10647" "cell_10436" "cell_11592" -[276] "cell_9817" "cell_11268" "cell_9961" "cell_10071" "cell_9959" -[281] "cell_11419" "cell_10585" "cell_11214" "cell_11084" "cell_11165" -[286] "cell_11327" "cell_11392" "cell_10718" "cell_10466" "cell_12090" -[291] "cell_11555" "cell_10995" "cell_10526" "cell_10761" "cell_11038" -[296] "cell_11232" "cell_10715" "cell_10458" "cell_10019" "cell_10328" -[301] "cell_10411" "cell_10313" "cell_9930" "cell_11114" "cell_9936" -[306] "cell_11234" "cell_10556" "cell_9846" "cell_11871" "cell_11285" -[311] "cell_10991" "cell_11939" "cell_10213" "cell_9863" "cell_11710" -[316] "cell_11256" "cell_10147" "cell_9989" "cell_10464" "cell_10953" -[321] "cell_10388" "cell_10216" "cell_10010" "cell_11485" "cell_10695" -[326] "cell_11760" "cell_11328" "cell_11620" "cell_10694" "cell_10175" -[331] "cell_9997" "cell_12147" "cell_10553" "cell_11266" "cell_10467" -[336] "cell_11233" "cell_10963" "cell_12149" "cell_9986" "cell_11276" -[341] "cell_11904" "cell_12179" "cell_9847" "cell_11303" "cell_11299" -[346] "cell_10956" "cell_10347" "cell_11599" "cell_10697" "cell_10190" -[351] "cell_10261" "cell_10498" "cell_11588" "cell_9977" "cell_11839" -[356] "cell_11777" "cell_11941" "cell_10645" "cell_10126" "cell_10833" -[361] "cell_10672" "cell_10654" "cell_11024" "cell_10164" "cell_11522" -[366] "cell_10407" "cell_12134" "cell_11056" "cell_11489" "cell_9969" -[371] "cell_9820" "cell_11017" "cell_9835" "cell_12064" "cell_11562" -[376] "cell_10345" "cell_10225" "cell_10783" "cell_12036" "cell_11333" -[381] "cell_10812" "cell_10998" "cell_9892" "cell_10360" "cell_12152" -[386] "cell_10508" "cell_9813" "cell_11124" "cell_11073" "cell_9859" -[391] "cell_10421" "cell_10930" "cell_10374" "cell_12097" "cell_11217" -[396] "cell_10800" "cell_11063" "cell_10363" "cell_10515" "cell_10267" -[401] "cell_11047" "cell_11744" "cell_11564" "cell_9849" "cell_11916" -[406] "cell_12082" "cell_12057" "cell_10664" "cell_11680" "cell_11542" -[411] "cell_10765" "cell_12066" "cell_11438" "cell_12072" "cell_10441" -[416] "cell_10396" "cell_10838" "cell_9832" "cell_10299" "cell_11769" -[421] "cell_11402" "cell_11736" "cell_11012" "cell_11484" "cell_11962" -[426] "cell_10604" "cell_11966" "cell_10370" "cell_10099" "cell_10669" -[431] "cell_11774" "cell_11579" "cell_10059" "cell_10511" "cell_10597" -[436] "cell_12031" "cell_10961" "cell_10497" "cell_11934" "cell_11353" -[441] "cell_11133" "cell_9837" "cell_11112" "cell_10272" "cell_10868" -[446] "cell_11734" "cell_10554" "cell_11683" "cell_10898" "cell_10623" -[451] "cell_10864" "cell_10217" "cell_10739" "cell_12174" "cell_12053" -[456] "cell_11859" "cell_10103" "cell_12167" "cell_10848" "cell_9975" -[461] "cell_10204" "cell_11678" "cell_10769" "cell_10369" "cell_10155" -[466] "cell_10860" "cell_10512" "cell_10333" "cell_11149" "cell_10560" -[471] "cell_10510" "cell_11658" "cell_10354" "cell_11141" "cell_11263" -[476] "cell_12058" "cell_9856" "cell_11208" "cell_9779" "cell_11598" -[481] "cell_10231" "cell_11547" "cell_10468" "cell_11961" "cell_10610" -[486] "cell_10453" "cell_10048" "cell_11304" "cell_10571" "cell_11778" -[491] "cell_10546" "cell_12111" "cell_11120" "cell_9940" "cell_12040" -[496] "cell_11471" "cell_11470" "cell_11321" "cell_10236" "cell_11176" -[501] "cell_12094" "cell_10509" "cell_10133" "cell_10359" "cell_11595" -[506] "cell_11019" "cell_10602" "cell_11414" "cell_11838" "cell_11932" -[511] "cell_11057" "cell_10303" "cell_10750" "cell_9880" "cell_10224" -[516] "cell_10885" "cell_11281" "cell_10399" "cell_9816" "cell_10737" -[521] "cell_10250" "cell_11040" "cell_9873" "cell_11782" "cell_10227" -[526] "cell_12125" "cell_11714" "cell_11104" "cell_11354" "cell_10660" -[531] "cell_9907" "cell_10249" "cell_10611" "cell_10018" "cell_11389" -[536] "cell_10098" "cell_10401" "cell_11418" "cell_12092" "cell_10890" -[541] "cell_10850" "cell_11791" "cell_11988" "cell_11897" "cell_10026" -[546] "cell_10403" "cell_10030" "cell_11688" "cell_11571" "cell_10696" -[551] "cell_9974" "cell_9967" "cell_11381" "cell_11972" "cell_12143" -[556] "cell_11837" "cell_10521" "cell_11280" "cell_11694" "cell_10166" -[561] "cell_11010" "cell_10947" "cell_11901" "cell_12044" "cell_10586" -[566] "cell_10428" "cell_10115" "cell_11357" "cell_11121" "cell_12114" -[571] "cell_12076" "cell_9823" "cell_11162" "cell_10836" "cell_11954" -[576] "cell_11713" "cell_11168" "cell_10700" "cell_11845" "cell_11557" -[581] "cell_11346" "cell_9962" "cell_11799" "cell_10869" "cell_11411" -[586] "cell_10766" "cell_11450" "cell_11058" "cell_10481" "cell_10123" -[591] "cell_10238" "cell_10107" "cell_9968" "cell_11951" "cell_11811" -[596] "cell_11519" "cell_11172" "cell_10661" "cell_10534" "cell_10904" -[601] "cell_12081" "cell_10237" "cell_10247" "cell_10380" "cell_11199" -[606] "cell_11447" "cell_11854" "cell_11524" "cell_10058" "cell_11586" -[611] "cell_10234" "cell_11743" "cell_10256" "cell_11775" "cell_11270" -[616] "cell_10089" "cell_9777" "cell_10635" "cell_10049" "cell_10410" -[621] "cell_10675" "cell_10476" "cell_10832" "cell_10940" "cell_10788" -[626] "cell_11818" "cell_11502" "cell_10731" "cell_12166" "cell_10646" -[631] "cell_11397" "cell_10151" "cell_12085" "cell_11435" "cell_10239" -[636] "cell_11715" "cell_12060" "cell_11798" "cell_9929" "cell_10189" -[641] "cell_10212" "cell_9797" "cell_11529" "cell_11033" "cell_11794" -[646] "cell_9812" "cell_9992" "cell_11696" "cell_10680" "cell_10319" -[651] "cell_10120" "cell_10601" "cell_12145" "cell_10451" "cell_11243" -[656] "cell_9824" "cell_11070" "cell_11983" "cell_11986" "cell_10422" -[661] "cell_11664" "cell_10143" "cell_11368" "cell_11580" "cell_11583" -[666] "cell_11312" "cell_11862" "cell_11493" "cell_11226" "cell_9785" -[671] "cell_10417" "cell_9931" "cell_11277" "cell_11261" "cell_11642" -[676] "cell_10977" "cell_10424" "cell_10588" "cell_9803" "cell_11938" -[681] "cell_11877" "cell_10271" "cell_10177" "cell_10437" "cell_10741" -[686] "cell_10580" "cell_11102" "cell_10945" "cell_11077" "cell_9916" -[691] "cell_9861" "cell_10037" "cell_10485" "cell_10443" "cell_10140" -[696] "cell_10285" "cell_11618" "cell_9845" "cell_11469" "cell_11140" -[701] "cell_10452" "cell_10352" "cell_9894" "cell_11823" "cell_10944" -[706] "cell_10488" "cell_10867" "cell_11516" "cell_12116" "cell_10755" -[711] "cell_10072" "cell_11393" "cell_11650" "cell_10878" "cell_11400" -[716] "cell_9868" "cell_10817" "cell_11700" "cell_11085" "cell_12007" -[721] "cell_11998" "cell_12158" "cell_10315" "cell_10698" "cell_10471" -[726] "cell_10111" "cell_11945" "cell_10999" "cell_11225" "cell_11083" -[731] "cell_11539" "cell_12113" "cell_11158" "cell_9802" "cell_11173" -[736] "cell_10034" "cell_10193" "cell_12083" "cell_11296" "cell_11007" -[741] "cell_10895" "cell_10725" "cell_11709" "cell_10626" "cell_10168" -[746] "cell_9770" "cell_10870" "cell_10950" "cell_9924" "cell_12006" -[751] "cell_9983" "cell_10787" "cell_10054" "cell_11118" "cell_10732" -[756] "cell_10198" "cell_11923" "cell_10518" "cell_10418" "cell_10484" -[761] "cell_10522" "cell_10153" "cell_11483" "cell_11309" "cell_11445" -[766] "cell_11754" "cell_11914" "cell_10677" "cell_11772" "cell_11697" -[771] "cell_9942" "cell_10202" "cell_10383" "cell_11074" "cell_11212" -[776] "cell_10572" "cell_11342" "cell_10593" "cell_10283" "cell_12056" -[781] "cell_9819" "cell_11771" "cell_11591" "cell_9998" "cell_11144" -[786] "cell_11015" "cell_10381" "cell_10937" "cell_11265" "cell_10086" -[791] "cell_10187" "cell_10215" "cell_11611" "cell_9911" "cell_11308" -[796] "cell_10735" "cell_11978" "cell_11067" "cell_10491" "cell_11003" -[801] "cell_10989" "cell_11480" "cell_10255" "cell_10312" "cell_11548" -[806] "cell_11207" "cell_9920" "cell_10209" "cell_11211" "cell_11422" -[811] "cell_11360" "cell_10305" "cell_10821" "cell_10461" "cell_10008" -[816] "cell_10801" "cell_11319" "cell_10078" "cell_11651" "cell_11287" -[821] "cell_10389" "cell_10135" "cell_10773" "cell_11246" "cell_11623" -[826] "cell_11627" "cell_11031" "cell_10200" "cell_11113" "cell_9794" -[831] "cell_12023" "cell_11197" "cell_11313" "cell_11129" "cell_11597" -[836] "cell_9807" "cell_10095" "cell_11860" - - -$`Somitic mesoderm` -$`Somitic mesoderm`$aucThr -$`Somitic mesoderm`$aucThr$selected -minimumDens - 0.0567 - -$`Somitic mesoderm`$aucThr$thresholds - threshold nCells -Global_k1 0.1638 171 -L_k2 0.1739 159 -minimumDens 0.0567 839 - -$`Somitic mesoderm`$aucThr$comment -[1] "The global distribution overlaps the partial distributions. " - - -$`Somitic mesoderm`$assignment - [1] "cell_11995" "cell_9963" "cell_10910" "cell_11021" "cell_10433" - [6] "cell_11395" "cell_10779" "cell_10883" "cell_10721" "cell_10116" - [11] "cell_10785" "cell_11781" "cell_11147" "cell_11842" "cell_11218" - [16] "cell_11441" "cell_11558" "cell_9979" "cell_10323" "cell_11663" - [21] "cell_10935" "cell_11567" "cell_10673" "cell_11634" "cell_10815" - [26] "cell_10077" "cell_9934" "cell_11888" "cell_10356" "cell_10490" - [31] "cell_9921" "cell_10062" "cell_9809" "cell_11906" "cell_10084" - [36] "cell_10951" "cell_10520" "cell_10142" "cell_12176" "cell_11850" - [41] "cell_10284" "cell_9781" "cell_11885" "cell_11687" "cell_10523" - [46] "cell_11100" "cell_10881" "cell_10774" "cell_12107" "cell_11216" - [51] "cell_10439" "cell_10902" "cell_10856" "cell_11702" "cell_11269" - [56] "cell_10248" "cell_11883" "cell_11682" "cell_10877" "cell_9933" - [61] "cell_10843" "cell_11154" "cell_12052" "cell_9895" "cell_12028" - [66] "cell_10454" "cell_11750" "cell_12059" "cell_10615" "cell_10751" - [71] "cell_10387" "cell_10245" "cell_11767" "cell_9919" "cell_11976" - [76] "cell_11971" "cell_10797" "cell_10094" "cell_11724" "cell_11030" - [81] "cell_11855" "cell_10754" "cell_9905" "cell_11530" "cell_11352" - [86] "cell_10357" "cell_12074" "cell_12012" "cell_11561" "cell_11398" - [91] "cell_12075" "cell_12177" "cell_11653" "cell_10644" "cell_11993" - [96] "cell_10322" "cell_11900" "cell_11315" "cell_10474" "cell_10480" -[101] "cell_11220" "cell_10440" "cell_11596" "cell_10296" "cell_11856" -[106] "cell_11728" "cell_10241" "cell_12016" "cell_11770" "cell_10917" -[111] "cell_11805" "cell_12002" "cell_10599" "cell_10846" "cell_10687" -[116] "cell_11330" "cell_11081" "cell_9953" "cell_10181" "cell_10395" -[121] "cell_12021" "cell_11667" "cell_10547" "cell_11329" "cell_10332" -[126] "cell_10562" "cell_11183" "cell_11138" "cell_10385" "cell_10125" -[131] "cell_10047" "cell_10038" "cell_10386" "cell_11673" "cell_10105" -[136] "cell_12162" "cell_10749" "cell_11549" "cell_11458" "cell_11290" -[141] "cell_11213" "cell_10849" "cell_11465" "cell_9885" "cell_10416" -[146] "cell_10847" "cell_11009" "cell_11733" "cell_10756" "cell_10479" -[151] "cell_11260" "cell_11002" "cell_10117" "cell_10933" "cell_11840" -[156] "cell_10922" "cell_11495" "cell_11380" "cell_10478" "cell_11026" -[161] "cell_11880" "cell_11896" "cell_12110" "cell_11115" "cell_10559" -[166] "cell_12151" "cell_11109" "cell_11014" "cell_11526" "cell_11018" -[171] "cell_11582" "cell_11476" "cell_10065" "cell_10628" "cell_11603" -[176] "cell_10825" "cell_9851" "cell_10634" "cell_10791" "cell_9844" -[181] "cell_10886" "cell_11931" "cell_11975" "cell_11150" "cell_10450" -[186] "cell_12115" "cell_11506" "cell_11239" "cell_11130" "cell_10011" -[191] "cell_11922" "cell_11827" "cell_10393" "cell_11982" "cell_10229" -[196] "cell_12140" "cell_9799" "cell_10907" "cell_11957" "cell_9784" -[201] "cell_11204" "cell_10292" "cell_11229" "cell_10692" "cell_11370" -[206] "cell_9972" "cell_10914" "cell_10027" "cell_10262" "cell_11657" -[211] "cell_11552" "cell_11123" "cell_10900" "cell_11093" "cell_9778" -[216] "cell_9938" "cell_11001" "cell_11619" "cell_12160" "cell_9876" -[221] "cell_9776" "cell_10394" "cell_10029" "cell_11377" "cell_11679" -[226] "cell_11054" "cell_11528" "cell_11913" "cell_11515" "cell_10435" -[231] "cell_10758" "cell_10220" "cell_10624" "cell_11716" "cell_11371" -[236] "cell_12042" "cell_10414" "cell_10828" "cell_10659" "cell_11424" -[241] "cell_11096" "cell_12171" "cell_11803" "cell_11404" "cell_10561" -[246] "cell_11180" "cell_10046" "cell_10009" "cell_9792" "cell_11853" -[251] "cell_11095" "cell_11511" "cell_11835" "cell_11463" "cell_10146" -[256] "cell_10317" "cell_11407" "cell_12173" "cell_10161" "cell_10438" -[261] "cell_10981" "cell_10079" "cell_11210" "cell_11174" "cell_10876" -[266] "cell_10087" "cell_11630" "cell_11434" "cell_9888" "cell_10367" -[271] "cell_11107" "cell_11994" "cell_10192" "cell_11822" "cell_10647" -[276] "cell_10436" "cell_11592" "cell_9817" "cell_11268" "cell_9961" -[281] "cell_10071" "cell_9959" "cell_11419" "cell_10585" "cell_11214" -[286] "cell_11084" "cell_11165" "cell_11327" "cell_11392" "cell_10718" -[291] "cell_10466" "cell_12090" "cell_11555" "cell_10995" "cell_10526" -[296] "cell_10761" "cell_11038" "cell_11232" "cell_10715" "cell_10458" -[301] "cell_10019" "cell_10328" "cell_10411" "cell_10313" "cell_9930" -[306] "cell_11114" "cell_9936" "cell_11234" "cell_10556" "cell_9846" -[311] "cell_11871" "cell_11285" "cell_10991" "cell_11939" "cell_10213" -[316] "cell_9863" "cell_11710" "cell_11256" "cell_10147" "cell_9989" -[321] "cell_10464" "cell_10953" "cell_10388" "cell_10010" "cell_11485" -[326] "cell_10695" "cell_11760" "cell_11328" "cell_11620" "cell_10694" -[331] "cell_10175" "cell_9997" "cell_12147" "cell_10553" "cell_11266" -[336] "cell_10467" "cell_11233" "cell_10963" "cell_12149" "cell_9986" -[341] "cell_11276" "cell_11904" "cell_12179" "cell_9847" "cell_11303" -[346] "cell_11299" "cell_10956" "cell_10347" "cell_11599" "cell_10697" -[351] "cell_10190" "cell_10261" "cell_10498" "cell_11588" "cell_9977" -[356] "cell_11839" "cell_11777" "cell_11941" "cell_10645" "cell_10833" -[361] "cell_10672" "cell_10654" "cell_11024" "cell_10164" "cell_11522" -[366] "cell_10407" "cell_12134" "cell_11056" "cell_11489" "cell_9969" -[371] "cell_9820" "cell_11017" "cell_9835" "cell_12064" "cell_11562" -[376] "cell_10345" "cell_10225" "cell_10783" "cell_12036" "cell_11333" -[381] "cell_10812" "cell_10998" "cell_9892" "cell_10360" "cell_12152" -[386] "cell_10508" "cell_9813" "cell_11124" "cell_11073" "cell_9859" -[391] "cell_10421" "cell_10930" "cell_10374" "cell_12097" "cell_11217" -[396] "cell_10800" "cell_11063" "cell_10363" "cell_10515" "cell_10267" -[401] "cell_11047" "cell_11744" "cell_11564" "cell_9849" "cell_11916" -[406] "cell_12082" "cell_12057" "cell_10664" "cell_11680" "cell_11542" -[411] "cell_10765" "cell_12066" "cell_11438" "cell_12072" "cell_10441" -[416] "cell_10396" "cell_10838" "cell_9832" "cell_10299" "cell_11402" -[421] "cell_11736" "cell_11012" "cell_11484" "cell_11962" "cell_10604" -[426] "cell_11966" "cell_10370" "cell_10099" "cell_10669" "cell_11774" -[431] "cell_11579" "cell_10059" "cell_10511" "cell_10597" "cell_12031" -[436] "cell_10961" "cell_10497" "cell_11934" "cell_11353" "cell_11133" -[441] "cell_9837" "cell_11112" "cell_10272" "cell_10868" "cell_11734" -[446] "cell_10554" "cell_11683" "cell_10898" "cell_10623" "cell_10864" -[451] "cell_10217" "cell_10739" "cell_12174" "cell_12053" "cell_11859" -[456] "cell_10103" "cell_12167" "cell_10848" "cell_9975" "cell_10204" -[461] "cell_11678" "cell_10769" "cell_10369" "cell_10155" "cell_10860" -[466] "cell_10512" "cell_10333" "cell_11149" "cell_10560" "cell_10510" -[471] "cell_11658" "cell_10354" "cell_11141" "cell_11263" "cell_12058" -[476] "cell_9856" "cell_11208" "cell_9779" "cell_11598" "cell_10231" -[481] "cell_11547" "cell_10468" "cell_11961" "cell_10610" "cell_10453" -[486] "cell_10048" "cell_11304" "cell_10571" "cell_11778" "cell_10546" -[491] "cell_12111" "cell_11120" "cell_9940" "cell_12040" "cell_11471" -[496] "cell_11470" "cell_11321" "cell_10236" "cell_11176" "cell_12094" -[501] "cell_10509" "cell_10133" "cell_10359" "cell_11595" "cell_11019" -[506] "cell_10602" "cell_11414" "cell_11838" "cell_11932" "cell_11057" -[511] "cell_10303" "cell_10750" "cell_9880" "cell_10224" "cell_10885" -[516] "cell_11281" "cell_10399" "cell_9816" "cell_10737" "cell_10250" -[521] "cell_11040" "cell_9873" "cell_11782" "cell_10227" "cell_12125" -[526] "cell_11714" "cell_11104" "cell_11354" "cell_10660" "cell_9907" -[531] "cell_10249" "cell_10611" "cell_10018" "cell_11389" "cell_10098" -[536] "cell_10401" "cell_11418" "cell_12092" "cell_10890" "cell_10850" -[541] "cell_11791" "cell_11988" "cell_11897" "cell_10026" "cell_10403" -[546] "cell_10030" "cell_11688" "cell_11571" "cell_10696" "cell_9974" -[551] "cell_9967" "cell_11381" "cell_11972" "cell_12143" "cell_11837" -[556] "cell_10521" "cell_11280" "cell_11694" "cell_10166" "cell_11010" -[561] "cell_10947" "cell_11901" "cell_12044" "cell_10586" "cell_10428" -[566] "cell_10115" "cell_11357" "cell_11121" "cell_12114" "cell_12076" -[571] "cell_9823" "cell_11162" "cell_10836" "cell_11954" "cell_11713" -[576] "cell_11168" "cell_10700" "cell_11845" "cell_11557" "cell_11346" -[581] "cell_9962" "cell_11386" "cell_11799" "cell_10869" "cell_11411" -[586] "cell_10766" "cell_11450" "cell_11058" "cell_10481" "cell_10123" -[591] "cell_10238" "cell_10107" "cell_9968" "cell_11951" "cell_11811" -[596] "cell_11519" "cell_11172" "cell_10661" "cell_10534" "cell_10904" -[601] "cell_12081" "cell_10237" "cell_10247" "cell_10380" "cell_11199" -[606] "cell_11447" "cell_11854" "cell_11524" "cell_10058" "cell_11586" -[611] "cell_10234" "cell_11743" "cell_10256" "cell_11775" "cell_11270" -[616] "cell_10089" "cell_9777" "cell_10635" "cell_10049" "cell_10410" -[621] "cell_10675" "cell_10476" "cell_10832" "cell_10940" "cell_10788" -[626] "cell_11818" "cell_11502" "cell_10731" "cell_12166" "cell_10646" -[631] "cell_11397" "cell_10151" "cell_12085" "cell_11435" "cell_10239" -[636] "cell_11715" "cell_12060" "cell_11798" "cell_9929" "cell_10189" -[641] "cell_10212" "cell_9797" "cell_11529" "cell_11033" "cell_11794" -[646] "cell_9812" "cell_9992" "cell_11696" "cell_10680" "cell_10319" -[651] "cell_10120" "cell_10601" "cell_12145" "cell_10451" "cell_11243" -[656] "cell_9824" "cell_11070" "cell_11983" "cell_11986" "cell_10422" -[661] "cell_11664" "cell_10143" "cell_11368" "cell_11580" "cell_11583" -[666] "cell_11312" "cell_11862" "cell_11493" "cell_11226" "cell_9785" -[671] "cell_10417" "cell_9931" "cell_11277" "cell_11261" "cell_11642" -[676] "cell_10977" "cell_10424" "cell_10588" "cell_9803" "cell_11938" -[681] "cell_11877" "cell_10271" "cell_10177" "cell_10437" "cell_10741" -[686] "cell_10580" "cell_11102" "cell_10945" "cell_11077" "cell_9916" -[691] "cell_9861" "cell_10037" "cell_10485" "cell_10443" "cell_10140" -[696] "cell_10285" "cell_11618" "cell_9845" "cell_11469" "cell_11140" -[701] "cell_10452" "cell_10352" "cell_9894" "cell_11823" "cell_10944" -[706] "cell_10488" "cell_10867" "cell_11516" "cell_12116" "cell_10755" -[711] "cell_10072" "cell_11393" "cell_11650" "cell_10878" "cell_11400" -[716] "cell_9868" "cell_10817" "cell_11700" "cell_11085" "cell_12007" -[721] "cell_11998" "cell_12158" "cell_10315" "cell_10698" "cell_10471" -[726] "cell_10111" "cell_11945" "cell_10999" "cell_11225" "cell_11083" -[731] "cell_11539" "cell_12113" "cell_11158" "cell_9802" "cell_11173" -[736] "cell_10034" "cell_10193" "cell_10831" "cell_12083" "cell_11296" -[741] "cell_11007" "cell_10895" "cell_10725" "cell_11709" "cell_10626" -[746] "cell_10168" "cell_9770" "cell_10870" "cell_10950" "cell_9924" -[751] "cell_12006" "cell_9983" "cell_10787" "cell_10054" "cell_11118" -[756] "cell_10732" "cell_10198" "cell_11923" "cell_10518" "cell_10418" -[761] "cell_10484" "cell_10522" "cell_10153" "cell_11483" "cell_11309" -[766] "cell_11445" "cell_11754" "cell_11914" "cell_10677" "cell_11772" -[771] "cell_11697" "cell_9942" "cell_10202" "cell_10383" "cell_11074" -[776] "cell_11212" "cell_10572" "cell_11342" "cell_10593" "cell_10283" -[781] "cell_12056" "cell_9819" "cell_11771" "cell_11591" "cell_9998" -[786] "cell_11144" "cell_11015" "cell_10381" "cell_10937" "cell_11265" -[791] "cell_10086" "cell_10187" "cell_10215" "cell_11611" "cell_9911" -[796] "cell_11308" "cell_10735" "cell_11978" "cell_11067" "cell_10491" -[801] "cell_11003" "cell_10989" "cell_11480" "cell_10255" "cell_10312" -[806] "cell_11548" "cell_11207" "cell_9920" "cell_10209" "cell_11211" -[811] "cell_11422" "cell_11360" "cell_10305" "cell_10821" "cell_10461" -[816] "cell_10008" "cell_10801" "cell_11319" "cell_10078" "cell_11651" -[821] "cell_11287" "cell_10389" "cell_10135" "cell_10773" "cell_11246" -[826] "cell_11623" "cell_11627" "cell_11031" "cell_10200" "cell_11113" -[831] "cell_9794" "cell_12023" "cell_11197" "cell_11313" "cell_11129" -[836] "cell_11597" "cell_9807" "cell_10095" "cell_11860" - - -$`Spinal cord` -$`Spinal cord`$aucThr -$`Spinal cord`$aucThr$selected -outlierOfGlobal - 0.433 - -$`Spinal cord`$aucThr$thresholds - threshold nCells -outlierOfGlobal 0.4333 0 -Global_k1 0.1939 239 -L_k2 0.1241 632 -R_k3 0.0913 754 - -$`Spinal cord`$aucThr$comment -[1] "The AUC might follow a normal distribution (random gene-set?). The right distribution is taller. " - - -$`Spinal cord`$assignment -character(0) -``` + ::: @@ -1893,7 +1003,7 @@ sce$leiden_clust <- clusterCells(sce, use.dimred = "PCA", plotReducedDim(sce, "UMAP", color_by = "leiden_clust") ``` - + ::: ::: @@ -1922,7 +1032,7 @@ names(19): Allantois Cardiomyocytes ... Spinal cord Surface ectoderm plotReducedDim(ref, dimred = "umap", color_by = "celltype") ``` - + ``` r # Repetitive work -> write a function diff --git a/eda_qc.md b/eda_qc.md index 3c13970..8c9294c 100644 --- a/eda_qc.md +++ b/eda_qc.md @@ -106,9 +106,9 @@ The distribution of total counts (called the unique molecular identifier or UMI A simple approach would be to apply a threshold on the total count to only retain those barcodes with large totals. However, this may unnecessarily discard libraries derived from cell types with low RNA content. ::: callout -Depending on your data source, identifying and discarding empty droplets may not be necessary. Some academic institutions have research cores dedicated to single cell work that perform the sample preparation and sequencing. Many of these cores will also perform empty droplet filtering and other initial QC steps. If the sequencing outputs were provided to you by someone else, make sure to communicate with them about what pre-processing steps have been performed, if any. +Depending on your data source, identifying and discarding empty droplets may not be necessary. Some academic institutions have research cores dedicated to single cell work that perform the sample preparation and sequencing. Many of these cores will also perform empty droplet filtering and other initial QC steps. Specific details on the steps in common pipelines like [10x Genomics' CellRanger](https://www.10xgenomics.com/support/software/cell-ranger/latest/tutorials) can usually be found in the documentation that came with the sequencing material. - +The main point is: if the sequencing outputs were provided to you by someone else, make sure to communicate with them about what pre-processing steps have been performed, if any. ::: :::: challenge diff --git a/fig/cell_type_annotation-rendered-auc-dist2-1.png b/fig/cell_type_annotation-rendered-auc-dist2-1.png new file mode 100644 index 0000000..e1e04a8 Binary files /dev/null and b/fig/cell_type_annotation-rendered-auc-dist2-1.png differ diff --git a/fig/cell_type_annotation-rendered-unnamed-chunk-7-1.png b/fig/cell_type_annotation-rendered-unnamed-chunk-7-1.png index f3ca7cb..73f876f 100644 Binary files a/fig/cell_type_annotation-rendered-unnamed-chunk-7-1.png and b/fig/cell_type_annotation-rendered-unnamed-chunk-7-1.png differ diff --git a/fig/cell_type_annotation-rendered-unnamed-chunk-8-1.png b/fig/cell_type_annotation-rendered-unnamed-chunk-8-1.png index 73f876f..82d3d6f 100644 Binary files a/fig/cell_type_annotation-rendered-unnamed-chunk-8-1.png and b/fig/cell_type_annotation-rendered-unnamed-chunk-8-1.png differ diff --git a/intro-sce.md b/intro-sce.md index a6f524d..db748cb 100644 --- a/intro-sce.md +++ b/intro-sce.md @@ -116,6 +116,17 @@ This class implements a data structure that stores all aspects of our single-cel +:::: spoiler + +### Before `SingleCellExperiment` + +Before `SingleCellExperiment`, coders working with single cell data would sometimes keep all of these components in separate objects e.g. a matrix of counts, a data.frame of sample metadata, a data.frame of gene annotations and so on. There were two main disadvantages to this sort of "from scratch" approach: + +1. Tons of book-keeping. If you performed a QC step that removed dead cells, you also had to remember to remove that same set of cells from the cell-wise metadata. Un-expressed genes were dropped? Don't forget to filter the gene metadata table too. +2. All the downstream steps had to be "from scratch" as well. All the data munging, analysis, and visualization code had to be customized to the idiosyncrasies of a given input set. + +:::: + Let's look at an example dataset. `WTChimeraData` comes from a study on mouse development. We can assign one sample to a `SingleCellExperiment` object named `sce` like so: @@ -261,7 +272,7 @@ Here we add a column called "conservation" that is just an integer sequence from ``` r -rowData(sce)$conservation = 1:nrow(sce) +rowData(sce)$conservation = rnorm(nrow(sce)) ``` This is just a made-up example with a simple sequence of numbers, but in practice its convenient to store any sort of gene-wise information in the columns of the rowData. @@ -384,21 +395,6 @@ altExpNames(0): ::::::::::::::::::::::::::::::::::::::::::::: -:::: challenge - -#### Extension Challenge 1 - -Before `SingleCellExperiment`, coders working with single cell data would sometimes keep all of these components in separate objects e.g. a matrix of counts, a data.frame of sample metadata, a data.frame of gene annotations and so on. What are the main disadvantages of this sort of "from scratch" approach? - -::: solution - -1. You have to do tons of book-keeping! If you perform a QC step that removes dead cells, now you also have to remember to remove that same set of cells from the cell-wise metadata. Dropped un-expressed genes? Don't forget to filter the gene metadata table too. - -2. All the downstream steps have to be "from scratch" as well! All the data munging, analysis, and visualization code has to be customized to the idiosyncrasies of your input. Agh! - -::: - -:::: :::::::::::::: checklist diff --git a/md5sum.txt b/md5sum.txt index ad41738..7ba160b 100644 --- a/md5sum.txt +++ b/md5sum.txt @@ -4,9 +4,9 @@ "config.yaml" "b0d664d3d6abdd0e98b16282e1c03107" "site/built/config.yaml" "2024-09-24" "index.md" "495939ddd3f110be3bbcd49b60f4a7ce" "site/built/index.md" "2024-09-24" "links.md" "8184cf4149eafbf03ce8da8ff0778c14" "site/built/links.md" "2024-09-24" -"episodes/intro-sce.Rmd" "709fc538c9872b9494fa37f1059ea4a0" "site/built/intro-sce.md" "2024-10-02" -"episodes/eda_qc.Rmd" "b4800ddfe2d5deb5047311658f254e6d" "site/built/eda_qc.md" "2024-10-02" -"episodes/cell_type_annotation.Rmd" "5bd585c6e4c6fc09a7443ce4da35899f" "site/built/cell_type_annotation.md" "2024-10-02" +"episodes/intro-sce.Rmd" "b704934867b22d804de1e0fa0a9600eb" "site/built/intro-sce.md" "2024-10-02" +"episodes/eda_qc.Rmd" "b21851ec0c9912dd9cb8a0cf230bcf9d" "site/built/eda_qc.md" "2024-10-02" +"episodes/cell_type_annotation.Rmd" "8fab6e0cbb60d6fe6a67a0004c5ce5ab" "site/built/cell_type_annotation.md" "2024-10-02" "episodes/multi-sample.Rmd" "4711a38fd8b29961424215dd17fb7528" "site/built/multi-sample.md" "2024-09-30" "episodes/large_data.Rmd" "f19fa53e9e63d4cb8fe0f6ab61c8fc3a" "site/built/large_data.md" "2024-10-02" "episodes/hca.Rmd" "3f2af9dc9e53fd617512a37db87f20a7" "site/built/hca.md" "2024-10-02"