From 10eea642d9c8d1c8687fd15159f373d8279aefc5 Mon Sep 17 00:00:00 2001
From: GitHub Actions <actions@github.com>
Date: Mon, 9 Sep 2024 03:21:12 +0000
Subject: [PATCH] site deploy

Auto-generated via {sandpaper}
Source  : 6e0421b533c1aa6d213810087b35ad38491e2b03
Branch  : md-outputs
Author  : GitHub Actions <actions@github.com>
Time    : 2024-09-09 03:20:03 +0000
Message : markdown source builds

Auto-generated via {sandpaper}
Source  : ce479189d69d75b1d7907fb3be10144395bfaa8c
Branch  : main
Author  : Andrew Ghazi <6763470+andrewGhazi@users.noreply.github.com>
Time    : 2024-09-09 03:05:01 +0000
Message : Merge pull request #27 from ccb-hms/add_exercises

Add exercises
---
 404.html                                      |   4 +-
 CODE_OF_CONDUCT.html                          |   2 +-
 LICENSE.html                                  |   2 +-
 aio.html                                      | 164 ++++++++++--------
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 cell_type_annotation.html                     |   2 +-
 eda_qc.html                                   |  14 +-
 hca.html                                      |  52 ++----
 images.html                                   |   4 +-
 index.html                                    |  14 +-
 instructor-notes.html                         |   4 +-
 instructor/404.html                           |   4 +-
 instructor/CODE_OF_CONDUCT.html               |   2 +-
 instructor/LICENSE.html                       |   2 +-
 instructor/aio.html                           | 164 ++++++++++--------
 instructor/cell_type_annotation.html          |   2 +-
 instructor/eda_qc.html                        |  14 +-
 instructor/hca.html                           |  52 ++----
 instructor/images.html                        |   4 +-
 instructor/index.html                         |  14 +-
 instructor/instructor-notes.html              |   4 +-
 instructor/intro-sce.html                     |   8 +-
 instructor/key-points.html                    |   4 +-
 instructor/large_data.html                    |  83 +++++++--
 instructor/multi-sample.html                  |  16 +-
 instructor/profiles.html                      |   4 +-
 instructor/reference.html                     |   2 +-
 intro-sce.html                                |   8 +-
 key-points.html                               |   4 +-
 large_data.html                               |  83 +++++++--
 md5sum.txt                                    |   4 +-
 multi-sample.html                             |  16 +-
 pkgdown.yml                                   |   2 +-
 profiles.html                                 |   4 +-
 reference.html                                |   2 +-
 64 files changed, 439 insertions(+), 325 deletions(-)
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diff --git a/404.html b/404.html
index 36a9297..77dc8f0 100644
--- a/404.html
+++ b/404.html
@@ -376,8 +376,8 @@ <h1>Page not found</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/404.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/404.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/CODE_OF_CONDUCT.html b/CODE_OF_CONDUCT.html
index 177f5d0..676f457 100644
--- a/CODE_OF_CONDUCT.html
+++ b/CODE_OF_CONDUCT.html
@@ -399,7 +399,7 @@ <h1>Contributor Code of Conduct</h1>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/CODE_OF_CONDUCT.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/LICENSE.html b/LICENSE.html
index 2f8fbaf..ad8c043 100644
--- a/LICENSE.html
+++ b/LICENSE.html
@@ -447,7 +447,7 @@ <h1>Licenses</h1>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/LICENSE.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/aio.html b/aio.html
index 1287678..26fb1bd 100644
--- a/aio.html
+++ b/aio.html
@@ -592,7 +592,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <ol style="list-style-type: decimal">
 <li><p>You have to do tons of book-keeping! If you perform a QC step
@@ -736,7 +736,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>Here we add a column called “conservation” that is just an integer
 sequence from 1 to the number of genes.</p>
@@ -815,7 +815,7 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> constructor function can be
 used to create a new <code>SingleCellExperiment</code> object.</p>
@@ -1036,7 +1036,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb4">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1116,7 +1116,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <button class="accordion-button spoiler-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSpoiler1" aria-expanded="false" aria-controls="collapseSpoiler1">
   <h3 class="accordion-header" id="headingSpoiler1">  <div class="note-square"><i aria-hidden="true" class="callout-icon" data-feather="eye"></i></div> Setting the Random Seed </h3>
 </button>
-<div id="collapseSpoiler1" class="accordion-collapse collapse" aria-labelledby="headingSpoiler1" data-bs-parent="#accordionSpoiler1">
+<div id="collapseSpoiler1" class="accordion-collapse collapse" data-bs-parent="#accordionSpoiler1" aria-labelledby="headingSpoiler1">
 <div class="accordion-body">
 <p>Whenever your code involves the generation of random numbers, it’s a
 good practice to set the random seed in R with
@@ -1349,7 +1349,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb25">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1625,7 +1625,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Spike-ins are deliberately-introduced exogeneous RNA from an exotic
 or synthetic source at a known concentration. This provides a known
@@ -1920,7 +1920,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <p>Mathematically, this would require the data to fall on a
 two-dimensional plane (for linear methods like PCA) or a smooth 2D
@@ -2090,7 +2090,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -3606,7 +3606,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>Samples 5 and 6 were from the same “pool” of cells. Looking at the
 documentation for the dataset under <code>?WTChimeraData</code> we see
@@ -3672,7 +3672,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>It’s important to have multiple samples within each experimental
 group because it helps the batch effect correction algorithm distinguish
@@ -4126,7 +4126,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>“logFC” stands for log fold-change. Rather than reporting e.g. a
 5-fold increase, it’s better to report a logFC of log(5) = 1.61.
@@ -4452,7 +4452,7 @@ <h3 class="callout-title">Exercise 1: Replicates</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>Remember, you can subset SingleCellExperiments with logical indices,
 just like a matrix. You can also access their column data with the
@@ -4466,7 +4466,7 @@ <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -4503,7 +4503,7 @@ <h3 class="callout-title">Exercise 2:</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
 <div class="accordion-body">
 <p>You can just hand <code>pheatmap()</code> a matrix as its only
 argument. It has a million options, but the defaults are usually pretty
@@ -4517,7 +4517,7 @@ <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution5" aria-expanded="false" aria-controls="collapseSolution5">
   <h4 class="accordion-header" id="headingSolution5"> Show me the solution </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" data-bs-parent="#accordionSolution5" aria-labelledby="headingSolution5">
+<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb53">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -4591,7 +4591,7 @@ <h3 class="callout-title">Key Points</h3>
  -->
 </section></section><section id="aio-large_data"><p>Content from <a href="large_data.html">Working with large data</a></p>
 <hr>
-<p>Last updated on 2024-09-06 |
+<p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -4672,7 +4672,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">TENxBrainData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce.brain</span> <span class="op">&lt;-</span> <span class="fu">TENxBrainData20k</span><span class="op">(</span><span class="op">)</span> </span>
+<span></span>
 <span><span class="va">sce.brain</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4766,7 +4768,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">counts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">log2</span><span class="op">(</span><span class="va">tmp</span> <span class="op">+</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4799,8 +4803,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scater</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">is.mito</span> <span class="op">&lt;-</span> <span class="fu">grepl</span><span class="op">(</span><span class="st">"^mt-"</span>, <span class="fu">rowData</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span><span class="op">$</span><span class="va">Symbol</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span> <span class="op">&lt;-</span> <span class="fu">perCellQCMetrics</span><span class="op">(</span><span class="va">sce.brain</span>, subsets <span class="op">=</span> <span class="fu">list</span><span class="op">(</span>Mt <span class="op">=</span> <span class="va">is.mito</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4874,9 +4881,10 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">param</span> <span class="op">&lt;-</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">bplapply</span><span class="op">(</span></span>
 <span>    X <span class="op">=</span> <span class="fu">c</span><span class="op">(</span><span class="fl">4</span>, <span class="fl">9</span>, <span class="fl">16</span>, <span class="fl">25</span><span class="op">)</span>,</span>
-<span>    FUN <span class="op">=</span> <span class="kw">function</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">{</span> <span class="fu">sqrt</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">}</span>,</span>
+<span>    FUN <span class="op">=</span> <span class="va">sqrt</span>,</span>
 <span>    BPPARAM <span class="op">=</span> <span class="va">param</span></span>
 <span><span class="op">)</span></span></code></pre>
 </div>
@@ -4906,10 +4914,15 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">MouseGastrulationData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span> <span class="op">&lt;-</span> <span class="fu">modelGeneVar</span><span class="op">(</span><span class="va">sce</span>, BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span><span class="fl">2</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4963,6 +4976,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># 2 hours, 8 GB, 1 CPU per task, for 10 tasks.</span></span>
 <span><span class="va">rs</span> <span class="op">&lt;-</span> <span class="fu">list</span><span class="op">(</span>walltime <span class="op">=</span> <span class="fl">7200</span>, memory <span class="op">=</span> <span class="fl">8000</span>, ncpus <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">bpp</span> <span class="op">&lt;-</span> <span class="fu">BatchtoolsParam</span><span class="op">(</span><span class="fl">10</span>, cluster <span class="op">=</span> <span class="st">"slurm"</span>, resources <span class="op">=</span> <span class="va">rs</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Parallelization is best suited for independent, CPU-intensive tasks
@@ -5035,7 +5049,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">bluster</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                               BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
@@ -5053,10 +5069,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">BiocNeighbors</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">clusters</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                         BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span>,</span>
 <span>                                                  BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">table</span><span class="op">(</span>exact <span class="op">=</span> <span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, approx <span class="op">=</span> <span class="va">clusters</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5065,19 +5084,19 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   2   0 391   1   0   0   0   1   0   3   0   0   0
-   6    0   0   0   0   0 206  51   0   0   0   1   0   0   0   0
-   7    0   0   0   0   0   3 194   0   0   1   0   0   0   0   0
-   8    0   0   0   0   2   0   0  91   0   0   0   2   0   0   0
+   4    0   0   0 342   0   0   0   0   0   0   0   0   0   0  55
+   5    0   0   0   0  74   0   0   0   0   0   0 198   0   0   0
+   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
    9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   10   0   0   0   0   0   0   0   0   0 113   8   0   0   0   0
-   11   0   0   0   0   0   0   0   0   0   0 144   0   0   0   0
-   12   0   0   0   0   2   0   0   0   0  15   0 199   0   0   0
-   13   0   0   0   0   0   0   0   0   0   0   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
+   10   0   0   0   0   0   0   0   0   0 113   0  16   0   0   0
+   11   0   0   0   0   0   0   0   0   0   0 153   0   0   0   0
+   12   0   0   2   0 321   0   0   0   0   1   0   0   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   0   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0</code></pre>
 </div>
 <p>The similarity of the two clusterings can be quantified by
 calculating the pairwise Rand index:</p>
@@ -5086,6 +5105,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">rand</span> <span class="op">&lt;-</span> <span class="fu">pairwiseRand</span><span class="op">(</span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, <span class="va">clusters</span>, mode <span class="op">=</span> <span class="st">"index"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">stopifnot</span><span class="op">(</span><span class="va">rand</span> <span class="op">&gt;</span> <span class="fl">0.8</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
@@ -5100,11 +5120,17 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y1</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y2</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">Y</span> <span class="op">&lt;-</span> <span class="fu">rbind</span><span class="op">(</span><span class="va">y1</span>, <span class="va">y2</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">exact</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">approx</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span>, BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">mean</span><span class="op">(</span><span class="va">exact</span><span class="op">$</span><span class="va">index</span> <span class="op">!=</span> <span class="va">approx</span><span class="op">$</span><span class="va">index</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5139,7 +5165,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <span></span>
 <span><span class="co"># As the name suggests, it is random, so we need to set the seed.</span></span>
 <span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">r.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">RandomParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">r.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5161,7 +5189,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101001</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">IrlbaParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">i.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5221,7 +5251,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">123</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">e.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">e.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5344,6 +5376,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">SeuratData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">InstallData</span><span class="op">(</span><span class="st">"pbmc3k"</span><span class="op">)</span></span></code></pre>
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
@@ -5354,9 +5387,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># Use PBMC3K from SeuratData</span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">LoadData</span><span class="op">(</span>ds <span class="op">=</span> <span class="st">"pbmc3k"</span>, type <span class="op">=</span> <span class="st">"pbmc3k.final"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">UpdateSeuratObject</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span> <span class="op">&lt;-</span> <span class="fu">as.SingleCellExperiment</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span></span></code></pre>
 </div>
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
@@ -5367,8 +5404,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span></span></code></pre>
 </div>
 <p>After some processing of the dataset, the actual conversion is
@@ -5378,7 +5418,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sobj</span> <span class="op">&lt;-</span> <span class="fu">as.Seurat</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">Idents</span><span class="op">(</span><span class="va">sobj</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="st">"celltype.mapped"</span></span>
+<span></span>
 <span><span class="va">sobj</span></span></code></pre>
 </div>
 </div>
@@ -5422,6 +5464,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">example_h5ad</span> <span class="op">&lt;-</span> <span class="fu">system.file</span><span class="op">(</span><span class="st">"extdata"</span>, <span class="st">"krumsiek11.h5ad"</span>,</span>
 <span>                            package <span class="op">=</span> <span class="st">"zellkonverter"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">readH5AD</span><span class="op">(</span><span class="va">example_h5ad</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5447,6 +5490,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">out.file</span> <span class="op">&lt;-</span> <span class="fu">tempfile</span><span class="op">(</span>fileext <span class="op">=</span> <span class="st">".h5ad"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">writeH5AD</span><span class="op">(</span><span class="va">sce</span>, file <span class="op">=</span> <span class="va">out.file</span><span class="op">)</span></span></code></pre>
 </div>
 <p>The resulting H5AD file can then be read into Python using scanpy’s
@@ -5722,15 +5766,18 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce.brain</span> <span class="op">=</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">SerialParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">2</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">3</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span></code></pre>
 </div>
@@ -5785,7 +5832,7 @@ <h3 class="callout-title">Key Points</h3>
 any links that you are not going to use.
  --></section></section><section id="aio-hca"><p>Content from <a href="hca.html">Accessing data from the Human Cell Atlas (HCA)</a></p>
 <hr>
-<p>Last updated on 2024-09-06 |
+<p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -6099,25 +6146,6 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 11 scRNA-seq                          4
 12 sci-RNA-seq                        1</code></pre>
 </div>
-</section><section><h2 class="section-heading" id="available-organisms">Available organisms<a class="anchor" aria-label="anchor" href="#available-organisms"></a>
-</h2>
-<hr class="half-width">
-<div class="codewrapper sourceCode" id="cb16">
-<h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">metadata</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">distinct</span><span class="op">(</span><span class="va">organism</span>, <span class="va">dataset_id</span><span class="op">)</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">count</span><span class="op">(</span><span class="va">organism</span><span class="op">)</span></span></code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="output" tabindex="0"><code># A tibble: 1 × 2
-  organism         n
-  &lt;chr&gt;        &lt;int&gt;
-1 Homo sapiens    63</code></pre>
-</div>
 <div class="section level3">
 <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA sequencing counts<a class="anchor" aria-label="anchor" href="#download-single-cell-rna-sequencing-counts"></a>
 </h3>
@@ -6128,7 +6156,7 @@ <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA seq
 ‘counts’ data.</p>
 <p>For the sake of demonstration, we’ll focus this small subset of
 samples:</p>
-<div class="codewrapper sourceCode" id="cb18">
+<div class="codewrapper sourceCode" id="cb16">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6143,7 +6171,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <div class="section level4">
 <h4 id="query-raw-counts">Query raw counts<a class="anchor" aria-label="anchor" href="#query-raw-counts"></a>
 </h4>
-<div class="codewrapper sourceCode" id="cb19">
+<div class="codewrapper sourceCode" id="cb17">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6174,7 +6202,7 @@ <h4 id="query-counts-scaled-per-million">Query counts scaled per million<a class
 </h4>
 <p>This is helpful if just few genes are of interest, as they can be
 compared across samples.</p>
-<div class="codewrapper sourceCode" id="cb21">
+<div class="codewrapper sourceCode" id="cb19">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6201,7 +6229,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <div class="section level4">
 <h4 id="extract-only-a-subset-of-genes">Extract only a subset of genes<a class="anchor" aria-label="anchor" href="#extract-only-a-subset-of-genes"></a>
 </h4>
-<div class="codewrapper sourceCode" id="cb23">
+<div class="codewrapper sourceCode" id="cb21">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6233,7 +6261,7 @@ <h4 id="extracting-counts-as-a-seurat-object">Extracting counts as a Seurat obje
 <p>If needed, the H5 <code>SingleCellExperiment</code> can be converted
 into a Seurat object. Note that it may take a long time and use a lot of
 memory depending on how many cells you are requesting.</p>
-<div class="codewrapper sourceCode" id="cb25">
+<div class="codewrapper sourceCode" id="cb23">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6255,7 +6283,7 @@ <h4 id="saving-as-hdf5">Saving as HDF5<a class="anchor" aria-label="anchor" href
 objects, if necessary, is to use
 <code>saveHDF5SummarizedExperiment</code> from the
 <code>HDF5Array</code> package.</p>
-<div class="codewrapper sourceCode" id="cb26">
+<div class="codewrapper sourceCode" id="cb24">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6283,9 +6311,9 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb27">
+<div class="codewrapper sourceCode" id="cb25">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6316,9 +6344,9 @@ <h3 class="callout-title">Exercise 2</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb28">
+<div class="codewrapper sourceCode" id="cb26">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6348,9 +6376,9 @@ <h3 class="callout-title">Exercise 3</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb29">
+<div class="codewrapper sourceCode" id="cb27">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6389,9 +6417,9 @@ <h3 class="callout-title">Exercise 4</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb30">
+<div class="codewrapper sourceCode" id="cb28">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6495,8 +6523,8 @@ <h3 class="callout-title">Key Points</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/aio.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/aio.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
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diff --git a/cell_type_annotation.html b/cell_type_annotation.html
index a423c5a..6cb89cd 100644
--- a/cell_type_annotation.html
+++ b/cell_type_annotation.html
@@ -1575,7 +1575,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/cell_type_annotation.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-08",
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diff --git a/eda_qc.html b/eda_qc.html
index f250bd7..10b1f52 100644
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+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <p>Mathematically, this would require the data to fall on a
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 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -1663,7 +1663,7 @@ <h3 class="callout-title">Further Reading</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/eda_qc.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
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+  "datePublished": "2024-09-09"
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   </script><script>
diff --git a/hca.html b/hca.html
index 071b6cc..3fd7775 100644
--- a/hca.html
+++ b/hca.html
@@ -273,7 +273,6 @@ <h2 class="accordion-header chapters" id="flush-headingEleven">
 <li><a href="#summarizing-the-metadata">Summarizing the metadata</a></li>
 <li><a href="#columns-available-in-the-metadata">Columns available in the metadata</a></li>
 <li><a href="#available-assays">Available assays</a></li>
-<li><a href="#available-organisms">Available organisms</a></li>
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@@ -339,7 +338,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
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         <h1>Accessing data from the Human Cell Atlas (HCA)</h1>
-        <p>Last updated on 2024-09-06 |
+        <p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 
@@ -615,23 +614,6 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 11 scRNA-seq                          4
 12 sci-RNA-seq                        1</code></pre>
 </div>
-</section><section><h2 class="section-heading" id="available-organisms">Available organisms<a class="anchor" aria-label="anchor" href="#available-organisms"></a></h2>
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-</h3>
-<pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">metadata</span> <span class="op">|&gt;</span></span>
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-<h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="output" tabindex="0"><code># A tibble: 1 × 2
-  organism         n
-  &lt;chr&gt;        &lt;int&gt;
-1 Homo sapiens    63</code></pre>
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 <div class="section level3">
 <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA sequencing counts<a class="anchor" aria-label="anchor" href="#download-single-cell-rna-sequencing-counts"></a></h3>
 <p>The data can be provided as either “counts” or counts per million
@@ -641,7 +623,7 @@ <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA seq
 ‘counts’ data.</p>
 <p>For the sake of demonstration, we’ll focus this small subset of
 samples:</p>
-<div class="codewrapper sourceCode" id="cb18">
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@@ -655,7 +637,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
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@@ -685,7 +667,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
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 <h4 id="extract-only-a-subset-of-genes">Extract only a subset of genes<a class="anchor" aria-label="anchor" href="#extract-only-a-subset-of-genes"></a></h4>
-<div class="codewrapper sourceCode" id="cb23">
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@@ -742,7 +724,7 @@ <h4 id="extracting-counts-as-a-seurat-object">Extracting counts as a Seurat obje
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@@ -762,7 +744,7 @@ <h4 id="saving-as-hdf5">Saving as HDF5<a class="anchor" aria-label="anchor" href
 objects, if necessary, is to use
 <code>saveHDF5SummarizedExperiment</code> from the
 <code>HDF5Array</code> package.</p>
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@@ -788,9 +770,9 @@ <h3 class="callout-title">Exercise 1</h3>
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   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
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@@ -821,9 +803,9 @@ <h3 class="callout-title">Exercise 2</h3>
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   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
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@@ -894,9 +876,9 @@ <h3 class="callout-title">Exercise 4</h3>
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-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
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 <pre class="downlit sourceCode r">
@@ -1008,8 +990,8 @@ <h3 class="callout-title">Key Points</h3>
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   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-06",
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+  "datePublished": "2024-09-09"
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diff --git a/images.html b/images.html
index bb6d3c1..5c035c1 100644
--- a/images.html
+++ b/images.html
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   "identifier": "https://ccb-hms.github.io/osca-carpentries/images.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
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+  "datePublished": "2024-09-09"
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   </script><script>
diff --git a/index.html b/index.html
index 6dfcdaf..90d08a2 100644
--- a/index.html
+++ b/index.html
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   If you already have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
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 version is available, quit RStudio, and download the latest version for
@@ -398,7 +398,7 @@ <h4 class="accordion-header" id="headingSolution2">
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   </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
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 <ul><li>Download R from the <a href="https://cran.r-project.org/bin/windows/base/release.htm" class="external-link">CRAN
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@@ -429,7 +429,7 @@ <h4 class="accordion-header" id="headingSolution3">
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   </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
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 <ul><li>Open RStudio, and click on “Help” &gt; “Check for updates”. If a new
 version is available, quit RStudio, and download the latest version for
@@ -453,7 +453,7 @@ <h4 class="accordion-header" id="headingSolution4">
   If you don’t have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <ul><li>Download R from the <a href="https://cran.r-project.org/bin/macosx/" class="external-link">CRAN website</a>.</li>
 <li>Select the <code>.pkg</code> file for the latest R version</li>
@@ -486,7 +486,7 @@ <h4 class="accordion-header" id="headingSolution5">
   Install R using your package manager and RStudio
   </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
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@@ -608,8 +608,8 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
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index 96f0839..b602275 100644
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index 59156dc..1af5b98 100644
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index 959c4d7..6a113f9 100644
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index e3a07bd..a806d56 100644
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index f7a8835..c43c746 100644
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@@ -595,7 +595,7 @@ <h3 class="callout-title">Challenge</h3>
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   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
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 <ol style="list-style-type: decimal">
 <li><p>You have to do tons of book-keeping! If you perform a QC step
@@ -739,7 +739,7 @@ <h3 class="callout-title">Challenge</h3>
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 <p>Here we add a column called “conservation” that is just an integer
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@@ -818,7 +818,7 @@ <h3 class="callout-title">Exercise 1</h3>
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@@ -1040,7 +1040,7 @@ <h3 class="callout-title">Challenge</h3>
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 <p>Whenever your code involves the generation of random numbers, it’s a
 good practice to set the random seed in R with
@@ -1353,7 +1353,7 @@ <h3 class="callout-title">Challenge</h3>
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 <p>Spike-ins are deliberately-introduced exogeneous RNA from an exotic
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@@ -1924,7 +1924,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <p>Mathematically, this would require the data to fall on a
 two-dimensional plane (for linear methods like PCA) or a smooth 2D
@@ -2094,7 +2094,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -3612,7 +3612,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>Samples 5 and 6 were from the same “pool” of cells. Looking at the
 documentation for the dataset under <code>?WTChimeraData</code> we see
@@ -3678,7 +3678,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>It’s important to have multiple samples within each experimental
 group because it helps the batch effect correction algorithm distinguish
@@ -4132,7 +4132,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>“logFC” stands for log fold-change. Rather than reporting e.g. a
 5-fold increase, it’s better to report a logFC of log(5) = 1.61.
@@ -4458,7 +4458,7 @@ <h3 class="callout-title">Exercise 1: Replicates</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>Remember, you can subset SingleCellExperiments with logical indices,
 just like a matrix. You can also access their column data with the
@@ -4472,7 +4472,7 @@ <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -4509,7 +4509,7 @@ <h3 class="callout-title">Exercise 2:</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
 <div class="accordion-body">
 <p>You can just hand <code>pheatmap()</code> a matrix as its only
 argument. It has a million options, but the defaults are usually pretty
@@ -4523,7 +4523,7 @@ <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution5" aria-expanded="false" aria-controls="collapseSolution5">
   <h4 class="accordion-header" id="headingSolution5"> Show me the solution </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" data-bs-parent="#accordionSolution5" aria-labelledby="headingSolution5">
+<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb53">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -4597,7 +4597,7 @@ <h3 class="callout-title">Key Points</h3>
  -->
 </section></section><section id="aio-large_data"><p>Content from <a href="large_data.html">Working with large data</a></p>
 <hr>
-<p>Last updated on 2024-09-06 |
+<p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 12 minutes</p>
@@ -4679,7 +4679,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">TENxBrainData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce.brain</span> <span class="op">&lt;-</span> <span class="fu">TENxBrainData20k</span><span class="op">(</span><span class="op">)</span> </span>
+<span></span>
 <span><span class="va">sce.brain</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4773,7 +4775,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">counts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">log2</span><span class="op">(</span><span class="va">tmp</span> <span class="op">+</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4806,8 +4810,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scater</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">is.mito</span> <span class="op">&lt;-</span> <span class="fu">grepl</span><span class="op">(</span><span class="st">"^mt-"</span>, <span class="fu">rowData</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span><span class="op">$</span><span class="va">Symbol</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span> <span class="op">&lt;-</span> <span class="fu">perCellQCMetrics</span><span class="op">(</span><span class="va">sce.brain</span>, subsets <span class="op">=</span> <span class="fu">list</span><span class="op">(</span>Mt <span class="op">=</span> <span class="va">is.mito</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4881,9 +4888,10 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">param</span> <span class="op">&lt;-</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">bplapply</span><span class="op">(</span></span>
 <span>    X <span class="op">=</span> <span class="fu">c</span><span class="op">(</span><span class="fl">4</span>, <span class="fl">9</span>, <span class="fl">16</span>, <span class="fl">25</span><span class="op">)</span>,</span>
-<span>    FUN <span class="op">=</span> <span class="kw">function</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">{</span> <span class="fu">sqrt</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">}</span>,</span>
+<span>    FUN <span class="op">=</span> <span class="va">sqrt</span>,</span>
 <span>    BPPARAM <span class="op">=</span> <span class="va">param</span></span>
 <span><span class="op">)</span></span></code></pre>
 </div>
@@ -4913,10 +4921,15 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">MouseGastrulationData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span> <span class="op">&lt;-</span> <span class="fu">modelGeneVar</span><span class="op">(</span><span class="va">sce</span>, BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span><span class="fl">2</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -4970,6 +4983,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># 2 hours, 8 GB, 1 CPU per task, for 10 tasks.</span></span>
 <span><span class="va">rs</span> <span class="op">&lt;-</span> <span class="fu">list</span><span class="op">(</span>walltime <span class="op">=</span> <span class="fl">7200</span>, memory <span class="op">=</span> <span class="fl">8000</span>, ncpus <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">bpp</span> <span class="op">&lt;-</span> <span class="fu">BatchtoolsParam</span><span class="op">(</span><span class="fl">10</span>, cluster <span class="op">=</span> <span class="st">"slurm"</span>, resources <span class="op">=</span> <span class="va">rs</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Parallelization is best suited for independent, CPU-intensive tasks
@@ -5042,7 +5056,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">bluster</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                               BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
@@ -5060,10 +5076,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">BiocNeighbors</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">clusters</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                         BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span>,</span>
 <span>                                                  BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">table</span><span class="op">(</span>exact <span class="op">=</span> <span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, approx <span class="op">=</span> <span class="va">clusters</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5072,19 +5091,19 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   2   0 391   1   0   0   0   1   0   3   0   0   0
-   6    0   0   0   0   0 206  51   0   0   0   1   0   0   0   0
-   7    0   0   0   0   0   3 194   0   0   1   0   0   0   0   0
-   8    0   0   0   0   2   0   0  91   0   0   0   2   0   0   0
+   4    0   0   0 342   0   0   0   0   0   0   0   0   0   0  55
+   5    0   0   0   0  74   0   0   0   0   0   0 198   0   0   0
+   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
    9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   10   0   0   0   0   0   0   0   0   0 113   8   0   0   0   0
-   11   0   0   0   0   0   0   0   0   0   0 144   0   0   0   0
-   12   0   0   0   0   2   0   0   0   0  15   0 199   0   0   0
-   13   0   0   0   0   0   0   0   0   0   0   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
+   10   0   0   0   0   0   0   0   0   0 113   0  16   0   0   0
+   11   0   0   0   0   0   0   0   0   0   0 153   0   0   0   0
+   12   0   0   2   0 321   0   0   0   0   1   0   0   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   0   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0</code></pre>
 </div>
 <p>The similarity of the two clusterings can be quantified by
 calculating the pairwise Rand index:</p>
@@ -5093,6 +5112,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">rand</span> <span class="op">&lt;-</span> <span class="fu">pairwiseRand</span><span class="op">(</span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, <span class="va">clusters</span>, mode <span class="op">=</span> <span class="st">"index"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">stopifnot</span><span class="op">(</span><span class="va">rand</span> <span class="op">&gt;</span> <span class="fl">0.8</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
@@ -5107,11 +5127,17 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y1</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y2</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">Y</span> <span class="op">&lt;-</span> <span class="fu">rbind</span><span class="op">(</span><span class="va">y1</span>, <span class="va">y2</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">exact</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">approx</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span>, BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">mean</span><span class="op">(</span><span class="va">exact</span><span class="op">$</span><span class="va">index</span> <span class="op">!=</span> <span class="va">approx</span><span class="op">$</span><span class="va">index</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5146,7 +5172,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <span></span>
 <span><span class="co"># As the name suggests, it is random, so we need to set the seed.</span></span>
 <span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">r.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">RandomParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">r.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5168,7 +5196,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101001</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">IrlbaParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">i.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5228,7 +5258,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">123</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">e.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">e.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5351,6 +5383,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">SeuratData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">InstallData</span><span class="op">(</span><span class="st">"pbmc3k"</span><span class="op">)</span></span></code></pre>
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
@@ -5361,9 +5394,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># Use PBMC3K from SeuratData</span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">LoadData</span><span class="op">(</span>ds <span class="op">=</span> <span class="st">"pbmc3k"</span>, type <span class="op">=</span> <span class="st">"pbmc3k.final"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">UpdateSeuratObject</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span> <span class="op">&lt;-</span> <span class="fu">as.SingleCellExperiment</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span></span></code></pre>
 </div>
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
@@ -5374,8 +5411,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span></span></code></pre>
 </div>
 <p>After some processing of the dataset, the actual conversion is
@@ -5385,7 +5425,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sobj</span> <span class="op">&lt;-</span> <span class="fu">as.Seurat</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">Idents</span><span class="op">(</span><span class="va">sobj</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="st">"celltype.mapped"</span></span>
+<span></span>
 <span><span class="va">sobj</span></span></code></pre>
 </div>
 </div>
@@ -5429,6 +5471,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">example_h5ad</span> <span class="op">&lt;-</span> <span class="fu">system.file</span><span class="op">(</span><span class="st">"extdata"</span>, <span class="st">"krumsiek11.h5ad"</span>,</span>
 <span>                            package <span class="op">=</span> <span class="st">"zellkonverter"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">readH5AD</span><span class="op">(</span><span class="va">example_h5ad</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -5454,6 +5497,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">out.file</span> <span class="op">&lt;-</span> <span class="fu">tempfile</span><span class="op">(</span>fileext <span class="op">=</span> <span class="st">".h5ad"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">writeH5AD</span><span class="op">(</span><span class="va">sce</span>, file <span class="op">=</span> <span class="va">out.file</span><span class="op">)</span></span></code></pre>
 </div>
 <p>The resulting H5AD file can then be read into Python using scanpy’s
@@ -5729,15 +5773,18 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce.brain</span> <span class="op">=</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">SerialParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">2</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">3</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span></code></pre>
 </div>
@@ -5792,7 +5839,7 @@ <h3 class="callout-title">Key Points</h3>
 any links that you are not going to use.
  --></section></section><section id="aio-hca"><p>Content from <a href="hca.html">Accessing data from the Human Cell Atlas (HCA)</a></p>
 <hr>
-<p>Last updated on 2024-09-06 |
+<p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 30 minutes</p>
@@ -6107,25 +6154,6 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 11 scRNA-seq                          4
 12 sci-RNA-seq                        1</code></pre>
 </div>
-</section><section><h2 class="section-heading" id="available-organisms">Available organisms<a class="anchor" aria-label="anchor" href="#available-organisms"></a>
-</h2>
-<hr class="half-width">
-<div class="codewrapper sourceCode" id="cb16">
-<h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">metadata</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">distinct</span><span class="op">(</span><span class="va">organism</span>, <span class="va">dataset_id</span><span class="op">)</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">count</span><span class="op">(</span><span class="va">organism</span><span class="op">)</span></span></code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="output" tabindex="0"><code># A tibble: 1 × 2
-  organism         n
-  &lt;chr&gt;        &lt;int&gt;
-1 Homo sapiens    63</code></pre>
-</div>
 <div class="section level3">
 <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA sequencing counts<a class="anchor" aria-label="anchor" href="#download-single-cell-rna-sequencing-counts"></a>
 </h3>
@@ -6136,7 +6164,7 @@ <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA seq
 ‘counts’ data.</p>
 <p>For the sake of demonstration, we’ll focus this small subset of
 samples:</p>
-<div class="codewrapper sourceCode" id="cb18">
+<div class="codewrapper sourceCode" id="cb16">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6151,7 +6179,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <div class="section level4">
 <h4 id="query-raw-counts">Query raw counts<a class="anchor" aria-label="anchor" href="#query-raw-counts"></a>
 </h4>
-<div class="codewrapper sourceCode" id="cb19">
+<div class="codewrapper sourceCode" id="cb17">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6182,7 +6210,7 @@ <h4 id="query-counts-scaled-per-million">Query counts scaled per million<a class
 </h4>
 <p>This is helpful if just few genes are of interest, as they can be
 compared across samples.</p>
-<div class="codewrapper sourceCode" id="cb21">
+<div class="codewrapper sourceCode" id="cb19">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6209,7 +6237,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <div class="section level4">
 <h4 id="extract-only-a-subset-of-genes">Extract only a subset of genes<a class="anchor" aria-label="anchor" href="#extract-only-a-subset-of-genes"></a>
 </h4>
-<div class="codewrapper sourceCode" id="cb23">
+<div class="codewrapper sourceCode" id="cb21">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6241,7 +6269,7 @@ <h4 id="extracting-counts-as-a-seurat-object">Extracting counts as a Seurat obje
 <p>If needed, the H5 <code>SingleCellExperiment</code> can be converted
 into a Seurat object. Note that it may take a long time and use a lot of
 memory depending on how many cells you are requesting.</p>
-<div class="codewrapper sourceCode" id="cb25">
+<div class="codewrapper sourceCode" id="cb23">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6263,7 +6291,7 @@ <h4 id="saving-as-hdf5">Saving as HDF5<a class="anchor" aria-label="anchor" href
 objects, if necessary, is to use
 <code>saveHDF5SummarizedExperiment</code> from the
 <code>HDF5Array</code> package.</p>
-<div class="codewrapper sourceCode" id="cb26">
+<div class="codewrapper sourceCode" id="cb24">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6291,9 +6319,9 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb27">
+<div class="codewrapper sourceCode" id="cb25">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6324,9 +6352,9 @@ <h3 class="callout-title">Exercise 2</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb28">
+<div class="codewrapper sourceCode" id="cb26">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6356,9 +6384,9 @@ <h3 class="callout-title">Exercise 3</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb29">
+<div class="codewrapper sourceCode" id="cb27">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6397,9 +6425,9 @@ <h3 class="callout-title">Exercise 4</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb30">
+<div class="codewrapper sourceCode" id="cb28">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -6503,8 +6531,8 @@ <h3 class="callout-title">Key Points</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/aio.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/aio.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/cell_type_annotation.html b/instructor/cell_type_annotation.html
index 13c597e..d750406 100644
--- a/instructor/cell_type_annotation.html
+++ b/instructor/cell_type_annotation.html
@@ -1577,7 +1577,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/cell_type_annotation.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/eda_qc.html b/instructor/eda_qc.html
index b368862..ebd4f18 100644
--- a/instructor/eda_qc.html
+++ b/instructor/eda_qc.html
@@ -488,7 +488,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb4">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -567,7 +567,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <button class="accordion-button spoiler-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSpoiler1" aria-expanded="false" aria-controls="collapseSpoiler1">
   <h3 class="accordion-header" id="headingSpoiler1">  <div class="note-square"><i aria-hidden="true" class="callout-icon" data-feather="eye"></i></div> Setting the Random Seed </h3>
 </button>
-<div id="collapseSpoiler1" class="accordion-collapse collapse" aria-labelledby="headingSpoiler1" data-bs-parent="#accordionSpoiler1">
+<div id="collapseSpoiler1" class="accordion-collapse collapse" data-bs-parent="#accordionSpoiler1" aria-labelledby="headingSpoiler1">
 <div class="accordion-body">
 <p>Whenever your code involves the generation of random numbers, it’s a
 good practice to set the random seed in R with
@@ -793,7 +793,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb25">
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@@ -1065,7 +1065,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Spike-ins are deliberately-introduced exogeneous RNA from an exotic
 or synthetic source at a known concentration. This provides a known
@@ -1351,7 +1351,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <p>Mathematically, this would require the data to fall on a
 two-dimensional plane (for linear methods like PCA) or a smooth 2D
@@ -1514,7 +1514,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -1665,7 +1665,7 @@ <h3 class="callout-title">Further Reading</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/eda_qc.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/hca.html b/instructor/hca.html
index 08c1745..a070f5d 100644
--- a/instructor/hca.html
+++ b/instructor/hca.html
@@ -273,7 +273,6 @@ <h2 class="accordion-header chapters" id="flush-headingEleven">
 <li><a href="#summarizing-the-metadata">Summarizing the metadata</a></li>
 <li><a href="#columns-available-in-the-metadata">Columns available in the metadata</a></li>
 <li><a href="#available-assays">Available assays</a></li>
-<li><a href="#available-organisms">Available organisms</a></li>
 <li><a href="#exercises">Exercises</a></li>
         </ul></div><!--/div.accordion-body-->
     </div><!--/div.accordion-collapse-->
@@ -339,7 +338,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Accessing data from the Human Cell Atlas (HCA)</h1>
-        <p>Last updated on 2024-09-06 |
+        <p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 
@@ -617,23 +616,6 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 11 scRNA-seq                          4
 12 sci-RNA-seq                        1</code></pre>
 </div>
-</section><section><h2 class="section-heading" id="available-organisms">Available organisms<a class="anchor" aria-label="anchor" href="#available-organisms"></a></h2>
-<hr class="half-width"><div class="codewrapper sourceCode" id="cb16">
-<h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">metadata</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">distinct</span><span class="op">(</span><span class="va">organism</span>, <span class="va">dataset_id</span><span class="op">)</span> <span class="op">|&gt;</span></span>
-<span>    <span class="fu">count</span><span class="op">(</span><span class="va">organism</span><span class="op">)</span></span></code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="output" tabindex="0"><code># A tibble: 1 × 2
-  organism         n
-  &lt;chr&gt;        &lt;int&gt;
-1 Homo sapiens    63</code></pre>
-</div>
 <div class="section level3">
 <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA sequencing counts<a class="anchor" aria-label="anchor" href="#download-single-cell-rna-sequencing-counts"></a></h3>
 <p>The data can be provided as either “counts” or counts per million
@@ -643,7 +625,7 @@ <h3 id="download-single-cell-rna-sequencing-counts">Download single-cell RNA seq
 ‘counts’ data.</p>
 <p>For the sake of demonstration, we’ll focus this small subset of
 samples:</p>
-<div class="codewrapper sourceCode" id="cb18">
+<div class="codewrapper sourceCode" id="cb16">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -657,7 +639,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <div class="section level4">
 <h4 id="query-raw-counts">Query raw counts<a class="anchor" aria-label="anchor" href="#query-raw-counts"></a></h4>
-<div class="codewrapper sourceCode" id="cb19">
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 <pre class="downlit sourceCode r">
@@ -687,7 +669,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <h4 id="query-counts-scaled-per-million">Query counts scaled per million<a class="anchor" aria-label="anchor" href="#query-counts-scaled-per-million"></a></h4>
 <p>This is helpful if just few genes are of interest, as they can be
 compared across samples.</p>
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@@ -713,7 +695,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </div>
 <div class="section level4">
 <h4 id="extract-only-a-subset-of-genes">Extract only a subset of genes<a class="anchor" aria-label="anchor" href="#extract-only-a-subset-of-genes"></a></h4>
-<div class="codewrapper sourceCode" id="cb23">
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 <pre class="downlit sourceCode r">
@@ -744,7 +726,7 @@ <h4 id="extracting-counts-as-a-seurat-object">Extracting counts as a Seurat obje
 <p>If needed, the H5 <code>SingleCellExperiment</code> can be converted
 into a Seurat object. Note that it may take a long time and use a lot of
 memory depending on how many cells you are requesting.</p>
-<div class="codewrapper sourceCode" id="cb25">
+<div class="codewrapper sourceCode" id="cb23">
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 <pre class="downlit sourceCode r">
@@ -764,7 +746,7 @@ <h4 id="saving-as-hdf5">Saving as HDF5<a class="anchor" aria-label="anchor" href
 objects, if necessary, is to use
 <code>saveHDF5SummarizedExperiment</code> from the
 <code>HDF5Array</code> package.</p>
-<div class="codewrapper sourceCode" id="cb26">
+<div class="codewrapper sourceCode" id="cb24">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -790,9 +772,9 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
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 </h3>
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@@ -823,9 +805,9 @@ <h3 class="callout-title">Exercise 2</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
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 </h3>
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@@ -855,9 +837,9 @@ <h3 class="callout-title">Exercise 3</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
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 </h3>
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@@ -896,9 +878,9 @@ <h3 class="callout-title">Exercise 4</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
-<div class="codewrapper sourceCode" id="cb30">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -1010,8 +992,8 @@ <h3 class="callout-title">Key Points</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/hca.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/hca.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/images.html b/instructor/images.html
index eafd3b9..69d4f5e 100644
--- a/instructor/images.html
+++ b/instructor/images.html
@@ -494,8 +494,8 @@ <h3 id="hca-figure-1">Figure 1</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/images.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/images.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/index.html b/instructor/index.html
index 69f5ef2..4ace307 100644
--- a/instructor/index.html
+++ b/instructor/index.html
@@ -457,7 +457,7 @@ <h4 class="accordion-header" id="headingSolution1">
   If you already have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <ul><li>Open RStudio, and click on “Help” &gt; “Check for updates”. If a new
 version is available, quit RStudio, and download the latest version for
@@ -483,7 +483,7 @@ <h4 class="accordion-header" id="headingSolution2">
   If you don’t have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <ul><li>Download R from the <a href="https://cran.r-project.org/bin/windows/base/release.htm" class="external-link">CRAN
 website</a>.</li>
@@ -514,7 +514,7 @@ <h4 class="accordion-header" id="headingSolution3">
   If you already have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <ul><li>Open RStudio, and click on “Help” &gt; “Check for updates”. If a new
 version is available, quit RStudio, and download the latest version for
@@ -538,7 +538,7 @@ <h4 class="accordion-header" id="headingSolution4">
   If you don’t have R and RStudio installed
   </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <ul><li>Download R from the <a href="https://cran.r-project.org/bin/macosx/" class="external-link">CRAN website</a>.</li>
 <li>Select the <code>.pkg</code> file for the latest R version</li>
@@ -571,7 +571,7 @@ <h4 class="accordion-header" id="headingSolution5">
   Install R using your package manager and RStudio
   </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
+<div id="collapseSolution5" class="accordion-collapse collapse" data-bs-parent="#accordionSolution5" aria-labelledby="headingSolution5">
 <div class="accordion-body">
 <ul><li>Follow the instructions for your distribution from <a href="https://cloud.r-project.org/bin/linux" class="external-link">CRAN</a>, they provide
 information to get the most recent version of R for common
@@ -693,8 +693,8 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/index.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/index.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/instructor-notes.html b/instructor/instructor-notes.html
index 90b3f8a..d48afaa 100644
--- a/instructor/instructor-notes.html
+++ b/instructor/instructor-notes.html
@@ -430,8 +430,8 @@ <h1>Instructor Notes</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/instructor-notes.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/instructor-notes.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/intro-sce.html b/instructor/intro-sce.html
index d695558..c5b6996 100644
--- a/instructor/intro-sce.html
+++ b/instructor/intro-sce.html
@@ -545,7 +545,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <ol style="list-style-type: decimal"><li><p>You have to do tons of book-keeping! If you perform a QC step
 that removes dead cells, now you also have to remember to remove that
@@ -684,7 +684,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>Here we add a column called “conservation” that is just an integer
 sequence from 1 to the number of genes.</p>
@@ -762,7 +762,7 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> constructor function can be
 used to create a new <code>SingleCellExperiment</code> object.</p>
@@ -879,7 +879,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/intro-sce.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/key-points.html b/instructor/key-points.html
index c1ce780..15fbe2b 100644
--- a/instructor/key-points.html
+++ b/instructor/key-points.html
@@ -540,8 +540,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/key-points.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/key-points.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/large_data.html b/instructor/large_data.html
index 1956472..f1c17b6 100644
--- a/instructor/large_data.html
+++ b/instructor/large_data.html
@@ -333,7 +333,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Working with large data</h1>
-        <p>Last updated on 2024-09-06 |
+        <p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 
@@ -414,7 +414,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">TENxBrainData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce.brain</span> <span class="op">&lt;-</span> <span class="fu">TENxBrainData20k</span><span class="op">(</span><span class="op">)</span> </span>
+<span></span>
 <span><span class="va">sce.brain</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -508,7 +510,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">counts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">log2</span><span class="op">(</span><span class="va">tmp</span> <span class="op">+</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -541,8 +545,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scater</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">is.mito</span> <span class="op">&lt;-</span> <span class="fu">grepl</span><span class="op">(</span><span class="st">"^mt-"</span>, <span class="fu">rowData</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span><span class="op">$</span><span class="va">Symbol</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span> <span class="op">&lt;-</span> <span class="fu">perCellQCMetrics</span><span class="op">(</span><span class="va">sce.brain</span>, subsets <span class="op">=</span> <span class="fu">list</span><span class="op">(</span>Mt <span class="op">=</span> <span class="va">is.mito</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -613,9 +620,10 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">param</span> <span class="op">&lt;-</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">bplapply</span><span class="op">(</span></span>
 <span>    X <span class="op">=</span> <span class="fu">c</span><span class="op">(</span><span class="fl">4</span>, <span class="fl">9</span>, <span class="fl">16</span>, <span class="fl">25</span><span class="op">)</span>,</span>
-<span>    FUN <span class="op">=</span> <span class="kw">function</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">{</span> <span class="fu">sqrt</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">}</span>,</span>
+<span>    FUN <span class="op">=</span> <span class="va">sqrt</span>,</span>
 <span>    BPPARAM <span class="op">=</span> <span class="va">param</span></span>
 <span><span class="op">)</span></span></code></pre>
 </div>
@@ -645,10 +653,15 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">MouseGastrulationData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span> <span class="op">&lt;-</span> <span class="fu">modelGeneVar</span><span class="op">(</span><span class="va">sce</span>, BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span><span class="fl">2</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -702,6 +715,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># 2 hours, 8 GB, 1 CPU per task, for 10 tasks.</span></span>
 <span><span class="va">rs</span> <span class="op">&lt;-</span> <span class="fu">list</span><span class="op">(</span>walltime <span class="op">=</span> <span class="fl">7200</span>, memory <span class="op">=</span> <span class="fl">8000</span>, ncpus <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">bpp</span> <span class="op">&lt;-</span> <span class="fu">BatchtoolsParam</span><span class="op">(</span><span class="fl">10</span>, cluster <span class="op">=</span> <span class="st">"slurm"</span>, resources <span class="op">=</span> <span class="va">rs</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Parallelization is best suited for independent, CPU-intensive tasks
@@ -771,7 +785,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">bluster</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                               BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
@@ -789,10 +805,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">BiocNeighbors</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">clusters</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                         BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span>,</span>
 <span>                                                  BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">table</span><span class="op">(</span>exact <span class="op">=</span> <span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, approx <span class="op">=</span> <span class="va">clusters</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -801,19 +820,19 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   2   0 391   1   0   0   0   1   0   3   0   0   0
-   6    0   0   0   0   0 206  51   0   0   0   1   0   0   0   0
-   7    0   0   0   0   0   3 194   0   0   1   0   0   0   0   0
-   8    0   0   0   0   2   0   0  91   0   0   0   2   0   0   0
+   4    0   0   0 342   0   0   0   0   0   0   0   0   0   0  55
+   5    0   0   0   0  74   0   0   0   0   0   0 198   0   0   0
+   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
    9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   10   0   0   0   0   0   0   0   0   0 113   8   0   0   0   0
-   11   0   0   0   0   0   0   0   0   0   0 144   0   0   0   0
-   12   0   0   0   0   2   0   0   0   0  15   0 199   0   0   0
-   13   0   0   0   0   0   0   0   0   0   0   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
+   10   0   0   0   0   0   0   0   0   0 113   0  16   0   0   0
+   11   0   0   0   0   0   0   0   0   0   0 153   0   0   0   0
+   12   0   0   2   0 321   0   0   0   0   1   0   0   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   0   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0</code></pre>
 </div>
 <p>The similarity of the two clusterings can be quantified by
 calculating the pairwise Rand index:</p>
@@ -822,6 +841,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">rand</span> <span class="op">&lt;-</span> <span class="fu">pairwiseRand</span><span class="op">(</span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, <span class="va">clusters</span>, mode <span class="op">=</span> <span class="st">"index"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">stopifnot</span><span class="op">(</span><span class="va">rand</span> <span class="op">&gt;</span> <span class="fl">0.8</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
@@ -836,11 +856,17 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y1</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y2</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">Y</span> <span class="op">&lt;-</span> <span class="fu">rbind</span><span class="op">(</span><span class="va">y1</span>, <span class="va">y2</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">exact</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">approx</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span>, BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">mean</span><span class="op">(</span><span class="va">exact</span><span class="op">$</span><span class="va">index</span> <span class="op">!=</span> <span class="va">approx</span><span class="op">$</span><span class="va">index</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -874,7 +900,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <span></span>
 <span><span class="co"># As the name suggests, it is random, so we need to set the seed.</span></span>
 <span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">r.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">RandomParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">r.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -896,7 +924,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101001</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">IrlbaParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">i.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -956,7 +986,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">123</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">e.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">e.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -1076,6 +1108,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">SeuratData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">InstallData</span><span class="op">(</span><span class="st">"pbmc3k"</span><span class="op">)</span></span></code></pre>
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
@@ -1086,9 +1119,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># Use PBMC3K from SeuratData</span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">LoadData</span><span class="op">(</span>ds <span class="op">=</span> <span class="st">"pbmc3k"</span>, type <span class="op">=</span> <span class="st">"pbmc3k.final"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">UpdateSeuratObject</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span> <span class="op">&lt;-</span> <span class="fu">as.SingleCellExperiment</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span></span></code></pre>
 </div>
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
@@ -1099,8 +1136,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span></span></code></pre>
 </div>
 <p>After some processing of the dataset, the actual conversion is
@@ -1110,7 +1150,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sobj</span> <span class="op">&lt;-</span> <span class="fu">as.Seurat</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">Idents</span><span class="op">(</span><span class="va">sobj</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="st">"celltype.mapped"</span></span>
+<span></span>
 <span><span class="va">sobj</span></span></code></pre>
 </div>
 </div>
@@ -1153,6 +1195,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">example_h5ad</span> <span class="op">&lt;-</span> <span class="fu">system.file</span><span class="op">(</span><span class="st">"extdata"</span>, <span class="st">"krumsiek11.h5ad"</span>,</span>
 <span>                            package <span class="op">=</span> <span class="st">"zellkonverter"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">readH5AD</span><span class="op">(</span><span class="va">example_h5ad</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -1178,6 +1221,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">out.file</span> <span class="op">&lt;-</span> <span class="fu">tempfile</span><span class="op">(</span>fileext <span class="op">=</span> <span class="st">".h5ad"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">writeH5AD</span><span class="op">(</span><span class="va">sce</span>, file <span class="op">=</span> <span class="va">out.file</span><span class="op">)</span></span></code></pre>
 </div>
 <p>The resulting H5AD file can then be read into Python using scanpy’s
@@ -1449,15 +1493,18 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce.brain</span> <span class="op">=</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">SerialParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">2</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">3</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span></code></pre>
 </div>
@@ -1562,8 +1609,8 @@ <h3 class="callout-title">Key Points</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/large_data.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/large_data.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/multi-sample.html b/instructor/multi-sample.html
index 15d1c73..f22d192 100644
--- a/instructor/multi-sample.html
+++ b/instructor/multi-sample.html
@@ -545,7 +545,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>Samples 5 and 6 were from the same “pool” of cells. Looking at the
 documentation for the dataset under <code>?WTChimeraData</code> we see
@@ -609,7 +609,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>It’s important to have multiple samples within each experimental
 group because it helps the batch effect correction algorithm distinguish
@@ -1059,7 +1059,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>“logFC” stands for log fold-change. Rather than reporting e.g. a
 5-fold increase, it’s better to report a logFC of log(5) = 1.61.
@@ -1376,7 +1376,7 @@ <h3 class="callout-title">Exercise 1: Replicates</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>Remember, you can subset SingleCellExperiments with logical indices,
 just like a matrix. You can also access their column data with the
@@ -1390,7 +1390,7 @@ <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1427,7 +1427,7 @@ <h3 class="callout-title">Exercise 2:</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
 <div class="accordion-body">
 <p>You can just hand <code>pheatmap()</code> a matrix as its only
 argument. It has a million options, but the defaults are usually pretty
@@ -1441,7 +1441,7 @@ <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution5" aria-expanded="false" aria-controls="collapseSolution5">
   <h4 class="accordion-header" id="headingSolution5"> Show me the solution </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" data-bs-parent="#accordionSolution5" aria-labelledby="headingSolution5">
+<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb53">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1567,7 +1567,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/multi-sample.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/profiles.html b/instructor/profiles.html
index df3ebb0..1b3f79a 100644
--- a/instructor/profiles.html
+++ b/instructor/profiles.html
@@ -352,8 +352,8 @@ <h1>Learner Profiles</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/profiles.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/profiles.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/instructor/reference.html b/instructor/reference.html
index 4ab7c02..568595b 100644
--- a/instructor/reference.html
+++ b/instructor/reference.html
@@ -413,7 +413,7 @@ <h1>Reference</h1>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/reference.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/intro-sce.html b/intro-sce.html
index d51961a..39f6f25 100644
--- a/intro-sce.html
+++ b/intro-sce.html
@@ -543,7 +543,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <ol style="list-style-type: decimal"><li><p>You have to do tons of book-keeping! If you perform a QC step
 that removes dead cells, now you also have to remember to remove that
@@ -682,7 +682,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>Here we add a column called “conservation” that is just an integer
 sequence from 1 to the number of genes.</p>
@@ -760,7 +760,7 @@ <h3 class="callout-title">Exercise 1</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> constructor function can be
 used to create a new <code>SingleCellExperiment</code> object.</p>
@@ -877,7 +877,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/intro-sce.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/key-points.html b/key-points.html
index 9b96a3e..85a5578 100644
--- a/key-points.html
+++ b/key-points.html
@@ -538,8 +538,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://ccb-hms.github.io/osca-carpentries/key-points.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/key-points.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/large_data.html b/large_data.html
index c2db90a..3ea8925 100644
--- a/large_data.html
+++ b/large_data.html
@@ -333,7 +333,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Working with large data</h1>
-        <p>Last updated on 2024-09-06 |
+        <p>Last updated on 2024-09-09 |
 
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 
@@ -412,7 +412,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">TENxBrainData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce.brain</span> <span class="op">&lt;-</span> <span class="fu">TENxBrainData20k</span><span class="op">(</span><span class="op">)</span> </span>
+<span></span>
 <span><span class="va">sce.brain</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -506,7 +508,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">counts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span> <span class="op">&lt;-</span> <span class="fu">log2</span><span class="op">(</span><span class="va">tmp</span> <span class="op">+</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">tmp</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -539,8 +543,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scater</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">is.mito</span> <span class="op">&lt;-</span> <span class="fu">grepl</span><span class="op">(</span><span class="st">"^mt-"</span>, <span class="fu">rowData</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span><span class="op">$</span><span class="va">Symbol</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span> <span class="op">&lt;-</span> <span class="fu">perCellQCMetrics</span><span class="op">(</span><span class="va">sce.brain</span>, subsets <span class="op">=</span> <span class="fu">list</span><span class="op">(</span>Mt <span class="op">=</span> <span class="va">is.mito</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">qcstats</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -611,9 +618,10 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">param</span> <span class="op">&lt;-</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">bplapply</span><span class="op">(</span></span>
 <span>    X <span class="op">=</span> <span class="fu">c</span><span class="op">(</span><span class="fl">4</span>, <span class="fl">9</span>, <span class="fl">16</span>, <span class="fl">25</span><span class="op">)</span>,</span>
-<span>    FUN <span class="op">=</span> <span class="kw">function</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">{</span> <span class="fu">sqrt</span><span class="op">(</span><span class="va">x</span><span class="op">)</span> <span class="op">}</span>,</span>
+<span>    FUN <span class="op">=</span> <span class="va">sqrt</span>,</span>
 <span>    BPPARAM <span class="op">=</span> <span class="va">param</span></span>
 <span><span class="op">)</span></span></code></pre>
 </div>
@@ -643,10 +651,15 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">MouseGastrulationData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span> <span class="op">&lt;-</span> <span class="fu">modelGeneVar</span><span class="op">(</span><span class="va">sce</span>, BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span><span class="fl">2</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">dec.mc</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -700,6 +713,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># 2 hours, 8 GB, 1 CPU per task, for 10 tasks.</span></span>
 <span><span class="va">rs</span> <span class="op">&lt;-</span> <span class="fu">list</span><span class="op">(</span>walltime <span class="op">=</span> <span class="fl">7200</span>, memory <span class="op">=</span> <span class="fl">8000</span>, ncpus <span class="op">=</span> <span class="fl">1</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">bpp</span> <span class="op">&lt;-</span> <span class="fu">BatchtoolsParam</span><span class="op">(</span><span class="fl">10</span>, cluster <span class="op">=</span> <span class="st">"slurm"</span>, resources <span class="op">=</span> <span class="va">rs</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Parallelization is best suited for independent, CPU-intensive tasks
@@ -769,7 +783,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">bluster</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                               BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
@@ -787,10 +803,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">scran</span><span class="op">)</span></span>
+<span></span>
 <span><span class="kw">library</span><span class="op">(</span><span class="va">BiocNeighbors</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">clusters</span> <span class="op">&lt;-</span> <span class="fu">clusterCells</span><span class="op">(</span><span class="va">sce</span>, use.dimred <span class="op">=</span> <span class="st">"PCA"</span>,</span>
 <span>                         BLUSPARAM <span class="op">=</span> <span class="fu">NNGraphParam</span><span class="op">(</span>cluster.fun <span class="op">=</span> <span class="st">"louvain"</span>,</span>
 <span>                                                  BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">table</span><span class="op">(</span>exact <span class="op">=</span> <span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, approx <span class="op">=</span> <span class="va">clusters</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -799,19 +818,19 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   2   0 391   1   0   0   0   1   0   3   0   0   0
-   6    0   0   0   0   0 206  51   0   0   0   1   0   0   0   0
-   7    0   0   0   0   0   3 194   0   0   1   0   0   0   0   0
-   8    0   0   0   0   2   0   0  91   0   0   0   2   0   0   0
+   4    0   0   0 342   0   0   0   0   0   0   0   0   0   0  55
+   5    0   0   0   0  74   0   0   0   0   0   0 198   0   0   0
+   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
    9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   10   0   0   0   0   0   0   0   0   0 113   8   0   0   0   0
-   11   0   0   0   0   0   0   0   0   0   0 144   0   0   0   0
-   12   0   0   0   0   2   0   0   0   0  15   0 199   0   0   0
-   13   0   0   0   0   0   0   0   0   0   0   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
+   10   0   0   0   0   0   0   0   0   0 113   0  16   0   0   0
+   11   0   0   0   0   0   0   0   0   0   0 153   0   0   0   0
+   12   0   0   2   0 321   0   0   0   0   1   0   0   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   0   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0</code></pre>
 </div>
 <p>The similarity of the two clusterings can be quantified by
 calculating the pairwise Rand index:</p>
@@ -820,6 +839,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">rand</span> <span class="op">&lt;-</span> <span class="fu">pairwiseRand</span><span class="op">(</span><span class="fu">colLabels</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span>, <span class="va">clusters</span>, mode <span class="op">=</span> <span class="st">"index"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">stopifnot</span><span class="op">(</span><span class="va">rand</span> <span class="op">&gt;</span> <span class="fl">0.8</span><span class="op">)</span></span></code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
@@ -834,11 +854,17 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y1</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">y2</span> <span class="op">&lt;-</span> <span class="fu">matrix</span><span class="op">(</span><span class="fu">rnorm</span><span class="op">(</span><span class="fl">50000</span><span class="op">)</span>, nrow <span class="op">=</span> <span class="fl">1000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">Y</span> <span class="op">&lt;-</span> <span class="fu">rbind</span><span class="op">(</span><span class="va">y1</span>, <span class="va">y2</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">exact</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">approx</span> <span class="op">&lt;-</span> <span class="fu">findKNN</span><span class="op">(</span><span class="va">Y</span>, k <span class="op">=</span> <span class="fl">20</span>, BNPARAM <span class="op">=</span> <span class="fu">AnnoyParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">mean</span><span class="op">(</span><span class="va">exact</span><span class="op">$</span><span class="va">index</span> <span class="op">!=</span> <span class="va">approx</span><span class="op">$</span><span class="va">index</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -872,7 +898,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <span></span>
 <span><span class="co"># As the name suggests, it is random, so we need to set the seed.</span></span>
 <span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101000</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">r.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">RandomParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">r.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -894,7 +922,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">101001</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">IrlbaParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">i.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -954,7 +984,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="fu">set.seed</span><span class="op">(</span><span class="fl">123</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">e.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">str</span><span class="op">(</span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">e.out</span>, <span class="st">"PCA"</span><span class="op">)</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -1074,6 +1106,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="kw">library</span><span class="op">(</span><span class="va">SeuratData</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">InstallData</span><span class="op">(</span><span class="st">"pbmc3k"</span><span class="op">)</span></span></code></pre>
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
@@ -1084,9 +1117,13 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="co"># Use PBMC3K from SeuratData</span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">LoadData</span><span class="op">(</span>ds <span class="op">=</span> <span class="st">"pbmc3k"</span>, type <span class="op">=</span> <span class="st">"pbmc3k.final"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span> <span class="op">&lt;-</span> <span class="fu">UpdateSeuratObject</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span> <span class="op">&lt;-</span> <span class="fu">as.SingleCellExperiment</span><span class="op">(</span><span class="va">pbmc</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">pbmc.sce</span></span></code></pre>
 </div>
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
@@ -1097,8 +1134,11 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">WTChimeraData</span><span class="op">(</span>samples <span class="op">=</span> <span class="fl">5</span>, type <span class="op">=</span> <span class="st">"processed"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="va">sce</span></span></code></pre>
 </div>
 <p>After some processing of the dataset, the actual conversion is
@@ -1108,7 +1148,9 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sobj</span> <span class="op">&lt;-</span> <span class="fu">as.Seurat</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">Idents</span><span class="op">(</span><span class="va">sobj</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="st">"celltype.mapped"</span></span>
+<span></span>
 <span><span class="va">sobj</span></span></code></pre>
 </div>
 </div>
@@ -1151,6 +1193,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">example_h5ad</span> <span class="op">&lt;-</span> <span class="fu">system.file</span><span class="op">(</span><span class="st">"extdata"</span>, <span class="st">"krumsiek11.h5ad"</span>,</span>
 <span>                            package <span class="op">=</span> <span class="st">"zellkonverter"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">readH5AD</span><span class="op">(</span><span class="va">example_h5ad</span><span class="op">)</span></span></code></pre>
 </div>
 <div class="codewrapper">
@@ -1176,6 +1219,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </h3>
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">out.file</span> <span class="op">&lt;-</span> <span class="fu">tempfile</span><span class="op">(</span>fileext <span class="op">=</span> <span class="st">".h5ad"</span><span class="op">)</span></span>
+<span></span>
 <span><span class="fu">writeH5AD</span><span class="op">(</span><span class="va">sce</span>, file <span class="op">=</span> <span class="va">out.file</span><span class="op">)</span></span></code></pre>
 </div>
 <p>The resulting H5AD file can then be read into Python using scanpy’s
@@ -1447,15 +1491,18 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <pre class="downlit sourceCode r">
 <code class="sourceCode R"><span><span class="va">sce.brain</span> <span class="op">=</span> <span class="fu">logNormCounts</span><span class="op">(</span><span class="va">sce.brain</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">SerialParam</span><span class="op">(</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">2</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span>
 <span></span>
-<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
+<span><span class="fu">system.time</span><span class="op">(</span><span class="op">{</span><span class="va">i.out</span> <span class="op">&lt;-</span> <span class="fu">runPCA</span><span class="op">(</span><span class="va">sce.brain</span>, </span>
+<span>                             ncomponents <span class="op">=</span> <span class="fl">20</span>, </span>
 <span>                             BSPARAM <span class="op">=</span> <span class="fu">ExactParam</span><span class="op">(</span><span class="op">)</span>,</span>
 <span>                             BPPARAM <span class="op">=</span> <span class="fu">MulticoreParam</span><span class="op">(</span>workers <span class="op">=</span> <span class="fl">3</span><span class="op">)</span><span class="op">)</span><span class="op">}</span><span class="op">)</span></span></code></pre>
 </div>
@@ -1560,8 +1607,8 @@ <h3 class="callout-title">Key Points</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/large_data.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/large_data.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/md5sum.txt b/md5sum.txt
index b39df98..7e50b5a 100644
--- a/md5sum.txt
+++ b/md5sum.txt
@@ -8,8 +8,8 @@
 "episodes/eda_qc.Rmd" "1e88f395d30778f4526532deea43eb03" "site/built/eda_qc.md" "2024-09-06"
 "episodes/cell_type_annotation.Rmd" "66af56b730aaa88e937bc1743afb471a" "site/built/cell_type_annotation.md" "2024-09-08"
 "episodes/multi-sample.Rmd" "2d38d9903358ea8a8067abd82a1f1f54" "site/built/multi-sample.md" "2024-09-08"
-"episodes/large_data.Rmd" "bbe443f474a0823122658effa2beb57e" "site/built/large_data.md" "2024-09-06"
-"episodes/hca.Rmd" "6db220495ae4ae56d33e4ca5b5f9b8ae" "site/built/hca.md" "2024-09-06"
+"episodes/large_data.Rmd" "b9710492c6792ea435778c4e42f27e02" "site/built/large_data.md" "2024-09-09"
+"episodes/hca.Rmd" "e01d3fd1e07f158bed08b72d657ae1d1" "site/built/hca.md" "2024-09-09"
 "instructors/instructor-notes.md" "cae72b6712578d74a49fea7513099f8c" "site/built/instructor-notes.md" "2024-09-06"
 "learners/reference.md" "40fc1d0be2412d2d9d434a5bc84e4de8" "site/built/reference.md" "2024-09-06"
 "learners/setup.md" "25772142a26fe3c0cebbe650f5683269" "site/built/setup.md" "2024-09-06"
diff --git a/multi-sample.html b/multi-sample.html
index 8d68080..6f29737 100644
--- a/multi-sample.html
+++ b/multi-sample.html
@@ -543,7 +543,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1"> Show me the solution </h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>Samples 5 and 6 were from the same “pool” of cells. Looking at the
 documentation for the dataset under <code>?WTChimeraData</code> we see
@@ -607,7 +607,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2"> Show me the solution </h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>It’s important to have multiple samples within each experimental
 group because it helps the batch effect correction algorithm distinguish
@@ -1057,7 +1057,7 @@ <h3 class="callout-title">Challenge</h3>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3"> Show me the solution </h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>“logFC” stands for log fold-change. Rather than reporting e.g. a
 5-fold increase, it’s better to report a logFC of log(5) = 1.61.
@@ -1374,7 +1374,7 @@ <h3 class="callout-title">Exercise 1: Replicates</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>Remember, you can subset SingleCellExperiments with logical indices,
 just like a matrix. You can also access their column data with the
@@ -1388,7 +1388,7 @@ <h4 class="accordion-header" id="headingHint1"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4"> Show me the solution </h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1425,7 +1425,7 @@ <h3 class="callout-title">Exercise 2:</h3>
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
 <div class="accordion-body">
 <p>You can just hand <code>pheatmap()</code> a matrix as its only
 argument. It has a million options, but the defaults are usually pretty
@@ -1439,7 +1439,7 @@ <h4 class="accordion-header" id="headingHint2"> Give me a hint </h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution5" aria-expanded="false" aria-controls="collapseSolution5">
   <h4 class="accordion-header" id="headingSolution5"> Show me the solution </h4>
 </button>
-<div id="collapseSolution5" class="accordion-collapse collapse" data-bs-parent="#accordionSolution5" aria-labelledby="headingSolution5">
+<div id="collapseSolution5" class="accordion-collapse collapse" aria-labelledby="headingSolution5" data-bs-parent="#accordionSolution5">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb53">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1565,7 +1565,7 @@ <h3 class="callout-title">Key Points</h3>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/multi-sample.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/pkgdown.yml b/pkgdown.yml
index 0c53800..dc76279 100644
--- a/pkgdown.yml
+++ b/pkgdown.yml
@@ -2,4 +2,4 @@ pandoc: 3.1.11
 pkgdown: 2.1.0
 pkgdown_sha: ~
 articles: {}
-last_built: 2024-09-08T16:59Z
+last_built: 2024-09-09T03:20Z
diff --git a/profiles.html b/profiles.html
index a34aedb..6bd625d 100644
--- a/profiles.html
+++ b/profiles.html
@@ -352,8 +352,8 @@ <h1>Learner Profiles</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/profiles.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/profiles.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-09-08",
-  "datePublished": "2024-09-08"
+  "dateModified": "2024-09-09",
+  "datePublished": "2024-09-09"
 }
 
   </script><script>
diff --git a/reference.html b/reference.html
index 1d7d3ae..b7dbfc5 100644
--- a/reference.html
+++ b/reference.html
@@ -411,7 +411,7 @@ <h1>Reference</h1>
   "identifier": "https://ccb-hms.github.io/osca-carpentries/reference.html",
   "dateCreated": "2024-01-10",
   "dateModified": "2024-09-06",
-  "datePublished": "2024-09-08"
+  "datePublished": "2024-09-09"
 }
 
   </script><script>