From 16489548e1c5d0dddc4fc15facc861ffd402e0fe Mon Sep 17 00:00:00 2001 From: GitHub Actions Date: Tue, 5 Nov 2024 07:13:27 +0000 Subject: [PATCH] markdown source builds Auto-generated via `{sandpaper}` Source : 525d01158a9cdfafb73a3214ccfd9e30d656aec2 Branch : main Author : Charlotte Soneson Time : 2024-11-05 06:47:50 +0000 Message : Merge pull request #115 from carpentries-incubator/update/packages Update 34 packages --- 03-import-annotate.md | 10 +- 04-exploratory-qc.md | 20 +- 07-gene-set-analysis.md | 32 +- config.yaml | 86 - ...exploratory-qc-rendered-pca-exercise-1.png | Bin 14956 -> 14969 bytes ...ial-expression-rendered-heatmap-time-1.png | Bin 22621 -> 22606 bytes ...gene-set-analysis-rendered-hypergeom-1.png | Bin 190993 -> 191047 bytes md5sum.txt | 36 +- renv.lock | 3065 ----------------- 9 files changed, 49 insertions(+), 3200 deletions(-) delete mode 100644 config.yaml delete mode 100644 renv.lock diff --git a/03-import-annotate.md b/03-import-annotate.md index c56e23bd..3b0aceef 100644 --- a/03-import-annotate.md +++ b/03-import-annotate.md @@ -778,22 +778,22 @@ other attached packages: [3] org.Mm.eg.db_3.19.1 AnnotationDbi_1.66.0 [5] SummarizedExperiment_1.34.0 Biobase_2.64.0 [7] MatrixGenerics_1.16.0 matrixStats_1.4.1 - [9] GenomicRanges_1.56.1 GenomeInfoDb_1.40.1 + [9] GenomicRanges_1.56.2 GenomeInfoDb_1.40.1 [11] IRanges_2.38.1 S4Vectors_0.42.1 [13] BiocGenerics_0.50.0 knitr_1.48 loaded via a namespace (and not attached): - [1] Matrix_1.7-0 bit_4.5.0 jsonlite_1.8.9 + [1] Matrix_1.7-1 bit_4.5.0 jsonlite_1.8.9 [4] highr_0.11 compiler_4.4.1 BiocManager_1.30.25 [7] renv_1.0.11 crayon_1.5.3 blob_1.2.4 [10] Biostrings_2.72.1 png_0.1-8 fastmap_1.2.0 [13] yaml_2.3.10 lattice_0.22-6 R6_2.5.1 [16] XVector_0.44.0 S4Arrays_1.4.1 DelayedArray_0.30.1 [19] GenomeInfoDbData_1.2.12 DBI_1.2.3 rlang_1.1.4 -[22] KEGGREST_1.44.1 cachem_1.1.0 xfun_0.47 -[25] bit64_4.0.5 memoise_2.0.1 SparseArray_1.4.8 +[22] KEGGREST_1.44.1 cachem_1.1.0 xfun_0.49 +[25] bit64_4.5.2 memoise_2.0.1 SparseArray_1.4.8 [28] RSQLite_2.3.7 cli_3.6.3 zlibbioc_1.50.0 -[31] grid_4.4.1 vctrs_0.6.5 evaluate_1.0.0 +[31] grid_4.4.1 vctrs_0.6.5 evaluate_1.0.1 [34] abind_1.4-8 httr_1.4.7 pkgconfig_2.0.3 [37] tools_4.4.1 UCSC.utils_1.0.0 ``` diff --git a/04-exploratory-qc.md b/04-exploratory-qc.md index 33e0843f..dca50034 100644 --- a/04-exploratory-qc.md +++ b/04-exploratory-qc.md @@ -454,7 +454,7 @@ other attached packages: [7] vsn_3.72.0 DESeq2_1.44.0 [9] SummarizedExperiment_1.34.0 Biobase_2.64.0 [11] MatrixGenerics_1.16.0 matrixStats_1.4.1 -[13] GenomicRanges_1.56.1 GenomeInfoDb_1.40.1 +[13] GenomicRanges_1.56.2 GenomeInfoDb_1.40.1 [15] IRanges_2.38.1 S4Vectors_0.42.1 [17] BiocGenerics_0.50.0 @@ -464,32 +464,32 @@ loaded via a namespace (and not attached): [7] mgcv_1.9-1 png_0.1-8 vctrs_0.6.5 [10] pkgconfig_2.0.3 shape_1.4.6.1 crayon_1.5.3 [13] fastmap_1.2.0 XVector_0.44.0 labeling_0.4.3 -[16] utf8_1.2.4 promises_1.3.0 shinyAce_0.4.2 -[19] UCSC.utils_1.0.0 preprocessCore_1.66.0 xfun_0.47 +[16] utf8_1.2.4 promises_1.3.0 shinyAce_0.4.3 +[19] UCSC.utils_1.0.0 preprocessCore_1.66.0 xfun_0.49 [22] cachem_1.1.0 zlibbioc_1.50.0 jsonlite_1.8.9 [25] listviewer_4.0.0 highr_0.11 later_1.3.2 [28] DelayedArray_0.30.1 BiocParallel_1.38.0 parallel_4.4.1 [31] cluster_2.1.6 R6_2.5.1 bslib_0.8.0 -[34] limma_3.60.4 jquerylib_0.1.4 Rcpp_1.0.13 +[34] limma_3.60.6 jquerylib_0.1.4 Rcpp_1.0.13-1 [37] iterators_1.0.14 knitr_1.48 httpuv_1.6.15 -[40] Matrix_1.7-0 splines_4.4.1 igraph_2.0.3 +[40] Matrix_1.7-1 splines_4.4.1 igraph_2.1.1 [43] tidyselect_1.2.1 abind_1.4-8 yaml_2.3.10 [46] doParallel_1.0.17 codetools_0.2-20 affy_1.82.0 [49] miniUI_0.1.1.1 lattice_0.22-6 tibble_3.2.1 -[52] shiny_1.9.1 withr_3.0.1 evaluate_1.0.0 +[52] shiny_1.9.1 withr_3.0.2 evaluate_1.0.1 [55] circlize_0.4.16 pillar_1.9.0 affyio_1.74.0 [58] BiocManager_1.30.25 renv_1.0.11 DT_0.33 [61] foreach_1.5.2 shinyjs_2.1.0 generics_0.1.3 [64] munsell_0.5.1 scales_1.3.0 xtable_1.8-4 -[67] glue_1.7.0 tools_4.4.1 colourpicker_1.3.0 -[70] locfit_1.5-9.10 colorspace_2.1-1 nlme_3.1-164 +[67] glue_1.8.0 tools_4.4.1 colourpicker_1.3.0 +[70] locfit_1.5-9.10 colorspace_2.1-1 nlme_3.1-166 [73] GenomeInfoDbData_1.2.12 vipor_0.4.7 cli_3.6.3 [76] fansi_1.0.6 viridisLite_0.4.2 S4Arrays_1.4.1 -[79] dplyr_1.1.4 gtable_0.3.5 rintrojs_0.3.4 +[79] dplyr_1.1.4 gtable_0.3.6 rintrojs_0.3.4 [82] sass_0.4.9 digest_0.6.37 SparseArray_1.4.8 [85] ggrepel_0.9.6 farver_2.1.2 rjson_0.2.23 [88] htmlwidgets_1.6.4 htmltools_0.5.8.1 lifecycle_1.0.4 -[91] shinyWidgets_0.8.6 httr_1.4.7 GlobalOptions_0.1.2 +[91] shinyWidgets_0.8.7 httr_1.4.7 GlobalOptions_0.1.2 [94] statmod_1.5.0 mime_0.12 ``` diff --git a/07-gene-set-analysis.md b/07-gene-set-analysis.md index 56a4287f..ada35351 100644 --- a/07-gene-set-analysis.md +++ b/07-gene-set-analysis.md @@ -500,9 +500,9 @@ microbenchmark( ``` output Unit: microseconds - expr min lq mean median uq max neval - fisher 247.441 253.1220 287.03204 261.883 275.659 2133.728 100 - hyper 1.453 1.6835 2.51601 1.999 3.061 9.678 100 + expr min lq mean median uq max neval + fisher 240.809 245.127 266.64734 250.292 258.6875 1433.724 100 + hyper 1.473 1.698 2.40966 2.625 2.8910 7.514 100 ``` It is very astonishing that `phyper()` is hundreds of times faster than @@ -1230,7 +1230,7 @@ resTimeGO = enrichGO(gene = timeDEgenes, ``` ``` output ---> Expected input gene ID: 22702,16578,19113,192287,14211,210529 +--> Expected input gene ID: 12380,99412,94221,18775,11674,378462 ``` ``` output @@ -1478,19 +1478,19 @@ mmu00592 alpha-Linolenic acid metabolism - Mus mus mmu04913 Ovarian steroidogenesis - Mus musculus (house mouse) mmu04061 Viral protein interaction with cytokine and cytokine receptor - Mus musculus (house mouse) GeneRatio BgRatio RichFactor FoldEnrichment zScore pvalue -mmu00590 16/460 89/9787 0.1797753 3.824915 5.945245 3.232256e-06 -mmu00591 12/460 55/9787 0.2181818 4.642055 6.015024 6.976725e-06 -mmu00565 11/460 48/9787 0.2291667 4.875770 5.977670 1.021306e-05 -mmu00592 8/460 25/9787 0.3200000 6.808348 6.457490 1.194030e-05 -mmu04913 12/460 64/9787 0.1875000 3.989266 5.328009 3.553165e-05 -mmu04061 14/460 95/9787 0.1473684 3.135423 4.644614 1.301689e-04 +mmu00590 16/454 89/9709 0.1797753 3.844578 5.970763 3.021082e-06 +mmu00591 12/454 55/9709 0.2181818 4.665919 6.038307 6.615368e-06 +mmu00565 11/454 48/9709 0.2291667 4.900835 6.000284 9.722179e-06 +mmu00592 8/454 25/9709 0.3200000 6.843348 6.479015 1.149555e-05 +mmu04913 12/454 64/9709 0.1875000 4.009774 5.350291 3.376203e-05 +mmu04061 14/454 95/9709 0.1473684 3.151542 4.667283 1.232101e-04 p.adjust qvalue -mmu00590 0.0009253733 0.0008012571 -mmu00591 0.0009253733 0.0008012571 -mmu00565 0.0009253733 0.0008012571 -mmu00592 0.0009253733 0.0008012571 -mmu04913 0.0022029625 0.0019074887 -mmu04061 0.0067253926 0.0058233450 +mmu00590 0.0008794095 0.0007593113 +mmu00591 0.0008794095 0.0007593113 +mmu00565 0.0008794095 0.0007593113 +mmu00592 0.0008794095 0.0007593113 +mmu04913 0.0020662361 0.0017840566 +mmu04061 0.0062837135 0.0054255662 geneID mmu00590 18783/19215/211429/329502/78390/19223/67103/242546/13118/18781/18784/11689/232889/15446/237625/11687 mmu00591 18783/211429/329502/78390/242546/18781/18784/13113/622127/232889/237625/11687 diff --git a/config.yaml b/config.yaml deleted file mode 100644 index 29544760..00000000 --- a/config.yaml +++ /dev/null @@ -1,86 +0,0 @@ -#------------------------------------------------------------ -# Values for this lesson. -#------------------------------------------------------------ - -# Which carpentry is this (swc, dc, lc, or cp)? -# swc: Software Carpentry -# dc: Data Carpentry -# lc: Library Carpentry -# cp: Carpentries (to use for instructor training for instance) -# incubator: The Carpentries Incubator -carpentry: 'incubator' -varnish: mblue9/varnish - -# Overall title for pages. -title: 'RNA-seq analysis with Bioconductor' - -# Date the lesson was created (YYYY-MM-DD, this is empty by default) -created: '2020-09-15' - -# Comma-separated list of keywords for the lesson -keywords: 'software, data, lesson, The Carpentries' - -# Life cycle stage of the lesson -# possible values: pre-alpha, alpha, beta, stable -life_cycle: 'beta' - -# License of the lesson -license: 'CC-BY 4.0' - -# Link to the source repository for this lesson -source: 'https://github.com/carpentries-incubator/bioc-rnaseq' - -# Default branch of your lesson -branch: 'main' - -# Who to contact if there are any issues -contact: 'charlotte.soneson@fmi.ch' - -# Navigation ------------------------------------------------ -# -# Use the following menu items to specify the order of -# individual pages in each dropdown section. Leave blank to -# include all pages in the folder. -# -# Example ------------- -# -# episodes: -# - introduction.md -# - first-steps.md -# -# learners: -# - setup.md -# -# instructors: -# - instructor-notes.md -# -# profiles: -# - one-learner.md -# - another-learner.md - -# Order of episodes in your lesson -episodes: -- 01-intro-to-rnaseq.Rmd -- 02-setup.Rmd -- 03-import-annotate.Rmd -- 04-exploratory-qc.Rmd -- 05-differential-expression.Rmd -- 06-extra-design.Rmd -- 07-gene-set-analysis.Rmd -- 08-next-steps.Rmd - -# Information for Learners -learners: - -# Information for Instructors -instructors: - -# Learner Profiles -profiles: - -# Customisation --------------------------------------------- -# -# This space below is where custom yaml items (e.g. pinning -# sandpaper and varnish versions) should live - -url: https://carpentries-incubator.github.io/bioc-rnaseq diff --git a/fig/04-exploratory-qc-rendered-pca-exercise-1.png b/fig/04-exploratory-qc-rendered-pca-exercise-1.png index 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