From 3e81a1a6f1d4afb631331d05212d010e4f40226d Mon Sep 17 00:00:00 2001 From: mhebrard Date: Fri, 1 Mar 2024 10:22:34 +0800 Subject: [PATCH 001/132] Test reducing AKT1 interval --- .../NA12878-chr14-AKT1.config | 4 +++ .../chr14_non_gap_regions.interval_list | 26 +++++++++++++++++++ 2 files changed, 30 insertions(+) create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.config b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.config index 6557e454..4e9e5085 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.config +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.config @@ -1,3 +1,7 @@ +params { + autosomes_non_gap_regions = "${projectDir}/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list" +} + process { withName: 'verifybamid2' { diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list new file mode 100644 index 00000000..8b2defac --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list @@ -0,0 +1,26 @@ +@HD VN:1.6 SO:coordinate +@SQ SN:chr14 LN:107043718 M5:e0f0eecc3bcab6178c62b6211565c807 UR:file:/Users/gnanakkan/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d7/208435b73576bd9dd7cc1a11c12341/hg38.fa +chr14 16000001 16022537 + . +chr14 16022638 16053976 + . +chr14 16054460 16061677 + . +chr14 16061994 16086625 + . +chr14 16089563 16096530 + . +chr14 16096631 16105376 + . +chr14 16113233 16130858 + . +chr14 16133336 16140527 + . +chr14 16140628 16228649 + . +chr14 16228750 16282882 + . +chr14 16282983 16346517 + . +chr14 16346618 16367287 + . +chr14 16367388 16374057 + . +chr14 16374158 16377402 + . +chr14 16377503 16400063 + . +chr14 16400164 16404348 + . +chr14 16404449 17538659 + . +chr14 17538760 17539099 + . +chr14 17539200 17539539 + . +chr14 17539640 17540837 + . +chr14 17540938 18173523 + . +chr14 18223524 18712644 + . +chr14 18862645 19511713 + . +chr14 19611714 106883718 + . From c0906ab1341293ab6c791c7d9138a08af71f99b7 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 11:37:33 +0800 Subject: [PATCH 002/132] add vcf workflow block --- main.nf | 16 ++++++++++++++++ 1 file changed, 16 insertions(+) diff --git a/main.nf b/main.nf index 58f5a3a9..3d2f2a4e 100644 --- a/main.nf +++ b/main.nf @@ -147,6 +147,22 @@ workflow { picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + + Channel + samples.branch { rec -> + def vcf_file = rec.vcf ? file( rec.vcf ) : null + + output: rec.biosample_id && vcf_file + def vcf_idx = file( "${rec.vcf}.tbi" ) + + return tuple( rec.biosample_id, vcf_file, vcf_idx ) + } + .set { vcf_inputs } + + bcftools_stats( vcf_inputs ) + picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) + count_variants ( vcf_inputs ) + // channel for samplelist input file type bam processed outputs Channel .empty() From cb87429bddacd9ca0d4e8f6a60ef89fe084d9c0f Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 12:04:52 +0800 Subject: [PATCH 003/132] vcf block test --- main.nf | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index 3d2f2a4e..0d16d818 100644 --- a/main.nf +++ b/main.nf @@ -157,11 +157,12 @@ workflow { return tuple( rec.biosample_id, vcf_file, vcf_idx ) } + .view() .set { vcf_inputs } - bcftools_stats( vcf_inputs ) - picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) - count_variants ( vcf_inputs ) + //bcftools_stats( vcf_inputs ) + //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) + //count_variants ( vcf_inputs ) // channel for samplelist input file type bam processed outputs Channel From 2002939ce5ccd90e72fc265a128479eaeee65c6b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 12:37:35 +0800 Subject: [PATCH 004/132] vcf qc process added --- main.nf | 12 ++++++++--- modules/CollectVariantCallingMetrics/main.nf | 19 ++++++++++++++++++ modules/bcftools/main.nf | 17 ++++++++++++++++ modules/count_variants/main.nf | 20 +++++++++++++++++++ .../params.yaml | 1 + 5 files changed, 66 insertions(+), 3 deletions(-) create mode 100644 modules/CollectVariantCallingMetrics/main.nf create mode 100644 modules/bcftools/main.nf create mode 100644 modules/count_variants/main.nf diff --git a/main.nf b/main.nf index 0d16d818..c4062e0b 100644 --- a/main.nf +++ b/main.nf @@ -92,6 +92,9 @@ include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_bam include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_cram } from './modules/CollectMultipleMetrics' include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_bam } from './modules/CollectWgsMetrics' include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from './modules/CollectWgsMetrics' +include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' +include { bcftools_stats } from './modules/bcftools' +include { count_variants } from './modules/count_variants' include { multiqc } from './modules/multiqc' /* @@ -144,8 +147,8 @@ workflow { picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) - picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + //picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + //picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) Channel @@ -162,8 +165,10 @@ workflow { //bcftools_stats( vcf_inputs ) //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) - //count_variants ( vcf_inputs ) + count_variants ( vcf_inputs ) + +/* // channel for samplelist input file type bam processed outputs Channel .empty() @@ -191,6 +196,7 @@ workflow { multiqc( multiqc_in ) +*/ } /* diff --git a/modules/CollectVariantCallingMetrics/main.nf b/modules/CollectVariantCallingMetrics/main.nf new file mode 100644 index 00000000..dfc0b8cc --- /dev/null +++ b/modules/CollectVariantCallingMetrics/main.nf @@ -0,0 +1,19 @@ +process picard_collect_variant_calling_metrics_vcf { + tag { sample } + + input: + tuple val(sample), path(vcf), path(tbi) + path(ref_dbsnp) + + output: + tuple val(sample), path("${sample}.metrics") + + script: + """ + picard CollectVariantCallingMetrics \ + I=${vcf} \ + O=${sample} \ + DBSNP=${ref_dbsnp} + """ + +} \ No newline at end of file diff --git a/modules/bcftools/main.nf b/modules/bcftools/main.nf new file mode 100644 index 00000000..cb1176f6 --- /dev/null +++ b/modules/bcftools/main.nf @@ -0,0 +1,17 @@ +process bcftools_stats { + + tag { sample } + + input: + tuple val(sample), path(vcf), path(tbi) + + output: + tuple val(sample), path("${sample}.pass.stats") + + """ + bcftools stats \\ + -f PASS \\ + "${vcf}" \\ + > "${sample}.pass.stats" + """ +} \ No newline at end of file diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf new file mode 100644 index 00000000..a562b86c --- /dev/null +++ b/modules/count_variants/main.nf @@ -0,0 +1,20 @@ +process count_variants { + + tag { sample } + + input: + tuple val(sample), path(vcf), path(tbi) + + output: + tuple val(sample), path("${sample}.variant_counts.json") + + script: + """ + echo "test vcf module" ${vcf} ${tbi} >${sample}.variant_counts.json + # count_variants.py \ + # --input_vcf ${vcf} \ + # --output_json ${sample}.variant_counts.json \ + # --loglevel DEBUG + """ + +} \ No newline at end of file diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml index 780599e3..fb95db46 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml @@ -2,3 +2,4 @@ samples: - biosample_id: NA12878-chr14-AKT1 aln: NA12878-chr14-AKT1.bam + vcf: NA12878-chr14-AKT1.vcf From 5407855b238e19888c574427a7fb6f4e411686bf Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 12:44:14 +0800 Subject: [PATCH 005/132] added compute resource for vcf qc process --- nextflow.config | 32 ++++++++++++++++++++++++++++++++ 1 file changed, 32 insertions(+) diff --git a/nextflow.config b/nextflow.config index b2e27ce4..818903d1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -80,6 +80,38 @@ process { mode: params.publish_mode, ] } + withName:bcftools_stats { + + cpus = 4 + disk = '1 GB' + memory = '1 GB' + + publishDir = [ + path: "${params.publishdir}/bcftools", + mode: params.publish_mode, + ] + } + withName:picard_collect_variant_calling_metrics_vcf { + + cpus = 4 + disk = '1 GB' + memory = '2 GB' + + publishDir = [ + path: "${params.publishdir}/CollectVariantCallingMetrics", + mode: params.publish_mode, + ] + } + withName:count_variants { + cpus = 4 + disk = '1 GB' + memory = '1 GB' + + publishDir = [ + path: "${params.publishdir}/count_variants", + mode: params.publish_mode, + ] + } withName: 'multiqc' { cpus = 1 From dfb687d97a10d24745cfde2b09e4c70d8482b53d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 12:47:23 +0800 Subject: [PATCH 006/132] vcf qc process initial test with aln --- main.nf | 1 + .../NA12878-chr14-AKT1.vcf | 1 + .../NA12878-chr14-AKT1.vcf.tbi | 1 + tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh | 2 +- 4 files changed, 4 insertions(+), 1 deletion(-) create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.tbi diff --git a/main.nf b/main.nf index c4062e0b..ae861282 100644 --- a/main.nf +++ b/main.nf @@ -113,6 +113,7 @@ workflow { vbi2_ud = file( params.vbi2_ud ) vbi2_bed = file( params.vbi2_bed ) vbi2_mean = file( params.vbi2_mean ) + //ref_dbsnp = file( params.ref_dbsnp ) inputs = new YamlSlurper().parse(file(params.inputs_list)) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf new file mode 100644 index 00000000..5d14d000 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf @@ -0,0 +1 @@ +test vcf diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.tbi b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.tbi new file mode 100644 index 00000000..6291a738 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.tbi @@ -0,0 +1 @@ +test tbi diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh index 610fa8ff..ad972d04 100755 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh @@ -6,7 +6,7 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -nextflow run ../../main.nf \ +/Users/gnanakkan/bin/nf-23.09.1-edge/nextflow run ../../main.nf \ -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config \ --inputs_list params.yaml \ -work-dir ./work \ From fe88209ea3c56a9af0caa0b9a0bf086cf607ca4c Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 4 Apr 2024 13:12:06 +0800 Subject: [PATCH 007/132] test dummy vcf file --- tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml | 1 - 1 file changed, 1 deletion(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml index fb95db46..780599e3 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml @@ -2,4 +2,3 @@ samples: - biosample_id: NA12878-chr14-AKT1 aln: NA12878-chr14-AKT1.bam - vcf: NA12878-chr14-AKT1.vcf From fa6c6bbaff1dc31bca99b08c7f7c7ab39e77c07b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 18:35:56 +0800 Subject: [PATCH 008/132] added vcf qc in metrics py --- bin/metrics/metrics.py | 122 ++++++++++++++++++ .../params.yaml | 1 + 2 files changed, 123 insertions(+) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index 59509095..5e7bc45e 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -157,3 +157,125 @@ def cross_contamination_rate(mqc, biosample_id): v = "NA" return k, v + +def pass_snps(mqc): + """ + The number of PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_het_snps(mqc): + """ + The number of PASS het SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_het_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + + +def pass_homalt_snps(mqc): + """ + The number of PASS hom alt SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_homalt_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + + +def pass_snp_het_hom(mqc): + """ + The het/hom ratio for PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_snp_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + + +def pass_del(mqc): + """ + The number of PASS deletions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_del"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_ins(mqc): + """ + The number of PASS insertions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_ins"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_snp_ts_tv(mqc): + """ + The transition to transversion ratio of passing bi-allelic SNPs. + + Source: count_variants.py (bcftools stats - tstv) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = next(iter(mqc["multiqc_npm_count_variants"].values())) + v = d["pass_snp_ts_tv"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml index 780599e3..fb95db46 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml @@ -2,3 +2,4 @@ samples: - biosample_id: NA12878-chr14-AKT1 aln: NA12878-chr14-AKT1.bam + vcf: NA12878-chr14-AKT1.vcf From 0f03e8dc78e45ffdd72178129bfb167b95bb8c0b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 18:41:12 +0800 Subject: [PATCH 009/132] added vcf qc in compile_metrics py --- bin/compile_metrics.py | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index ff09d903..9e7221cf 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -54,6 +54,10 @@ def calculate_metrics(mqc): "mean_insert_size", "insert_size_std_deviation", "cross_contamination_rate" + # variant calling + "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", + "pass_del", "pass_ins", + "pass_snp_ts_tv" ] result = dict() logging.info("Calculating %d metrics..." % len(metrics_list)) From 69835940bbd58d1e83a268b6cd6d1a15e61d67fa Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 19:31:20 +0800 Subject: [PATCH 010/132] add autosomes_non_gap_regions --- main.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index ae861282..1b868425 100644 --- a/main.nf +++ b/main.nf @@ -110,6 +110,7 @@ workflow { ref_fasta = file( params.reference ) ref_fasta_idx = file( params.reference + ".fai" ) autosomes_non_gap_regions = file( params.autosomes_non_gap_regions ) + autosomes_non_gap_regions_bed = file( params.autosomes_non_gap_regions_bed ) vbi2_ud = file( params.vbi2_ud ) vbi2_bed = file( params.vbi2_bed ) vbi2_mean = file( params.vbi2_mean ) @@ -164,9 +165,9 @@ workflow { .view() .set { vcf_inputs } - //bcftools_stats( vcf_inputs ) + bcftools_stats( vcf_inputs ) //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) - count_variants ( vcf_inputs ) + count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) /* From 34f011fab2684fe12e89f072d54cfaf4c4f02041 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 19:33:02 +0800 Subject: [PATCH 011/132] add autosomes_non_gap_regions_bed in resources folder --- conf/resources.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/resources.config b/conf/resources.config index 10b9ce43..3e616f4e 100644 --- a/conf/resources.config +++ b/conf/resources.config @@ -10,4 +10,5 @@ params { vbi2_bed = "${projectDir}/resources/verifybamid/1000g.phase3.100k.b38.vcf.gz.dat.bed" vbi2_mean = "${projectDir}/resources/verifybamid/1000g.phase3.100k.b38.vcf.gz.dat.mu" autosomes_non_gap_regions = "${projectDir}/resources/autosomes_non_gap_regions.interval_list" + autosomes_non_gap_regions_bed = "${projectDir}/resources/autosomes_non_gap_regions.bed" } From 73722f6b4a4b5dae29feeddd13f4826fef235036 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 19:34:48 +0800 Subject: [PATCH 012/132] added autosomes_non_gap_regions.bed file --- resources/autosomes_non_gap_regions.bed | 3578 +++++++++++++++++++++++ 1 file changed, 3578 insertions(+) create mode 100644 resources/autosomes_non_gap_regions.bed diff --git a/resources/autosomes_non_gap_regions.bed b/resources/autosomes_non_gap_regions.bed new file mode 100644 index 00000000..09ecb69d --- /dev/null +++ b/resources/autosomes_non_gap_regions.bed @@ -0,0 +1,3578 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + NPM-sample-qc/autosomes_non_gap_regions.bed at feature/metrics-fix · c-BIG/NPM-sample-qc · GitHub + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
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+ +
+ + + From 4b1117dd0e85e28ee165c2729f255c59daff673a Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 19:39:03 +0800 Subject: [PATCH 013/132] adde autosomes regions to the arg --- modules/count_variants/main.nf | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index a562b86c..61e0ba01 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -3,18 +3,19 @@ process count_variants { tag { sample } input: - tuple val(sample), path(vcf), path(tbi) + tuple val(sample), path(vcf), path(tbi), path(autosomes_non_gap_regions) output: tuple val(sample), path("${sample}.variant_counts.json") script: """ - echo "test vcf module" ${vcf} ${tbi} >${sample}.variant_counts.json - # count_variants.py \ - # --input_vcf ${vcf} \ - # --output_json ${sample}.variant_counts.json \ - # --loglevel DEBUG + # echo "test vcf module" ${vcf} ${tbi} >${sample}.variant_counts.json + count_variants.py \ + --input_vcf ${vcf} \ + --regions ${autosomes_non_gap_regions} \ + --output_json ${sample}.variant_counts.json \ + --loglevel DEBUG """ } \ No newline at end of file From 88fa29a772ecbe1294340a0363a08f0be51b3426 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 20:09:47 +0800 Subject: [PATCH 014/132] add vcf qc code metrics py --- bin/metrics/metrics.py | 28 ++++++++++++++-------------- 1 file changed, 14 insertions(+), 14 deletions(-) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index 5e7bc45e..d8c2bff0 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -158,7 +158,7 @@ def cross_contamination_rate(mqc, biosample_id): return k, v -def pass_snps(mqc): +def pass_snps(mqc, biosample_id): """ The number of PASS SNPs. @@ -167,7 +167,7 @@ def pass_snps(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_snps"] v = int(v) except KeyError: @@ -175,7 +175,7 @@ def pass_snps(mqc): return k, v -def pass_het_snps(mqc): +def pass_het_snps(mqc, biosample_id): """ The number of PASS het SNPs. @@ -184,7 +184,7 @@ def pass_het_snps(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_het_snps"] v = int(v) except KeyError: @@ -193,7 +193,7 @@ def pass_het_snps(mqc): return k, v -def pass_homalt_snps(mqc): +def pass_homalt_snps(mqc, biosample_id): """ The number of PASS hom alt SNPs. @@ -202,7 +202,7 @@ def pass_homalt_snps(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_homalt_snps"] v = int(v) except KeyError: @@ -211,7 +211,7 @@ def pass_homalt_snps(mqc): return k, v -def pass_snp_het_hom(mqc): +def pass_snp_het_hom(mqc, biosample_id): """ The het/hom ratio for PASS SNPs. @@ -220,7 +220,7 @@ def pass_snp_het_hom(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_snp_het_hom"] v = np.round(v, DECIMALS) except KeyError: @@ -229,7 +229,7 @@ def pass_snp_het_hom(mqc): return k, v -def pass_del(mqc): +def pass_del(mqc, biosample_id): """ The number of PASS deletions. @@ -238,7 +238,7 @@ def pass_del(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_del"] v = int(v) except KeyError: @@ -246,7 +246,7 @@ def pass_del(mqc): return k, v -def pass_ins(mqc): +def pass_ins(mqc, biosample_id): """ The number of PASS insertions. @@ -255,7 +255,7 @@ def pass_ins(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_ins"] v = int(v) except KeyError: @@ -263,7 +263,7 @@ def pass_ins(mqc): return k, v -def pass_snp_ts_tv(mqc): +def pass_snp_ts_tv(mqc, biosample_id): """ The transition to transversion ratio of passing bi-allelic SNPs. @@ -272,7 +272,7 @@ def pass_snp_ts_tv(mqc): k = inspect.currentframe().f_code.co_name try: - d = next(iter(mqc["multiqc_npm_count_variants"].values())) + d = mqc["multiqc_npm_count_variants"][biosample_id] v = d["pass_snp_ts_tv"] v = np.round(v, DECIMALS) except KeyError: From 361464e3ca97aa3b35db49f2c7a418f15d5ed438 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 21:32:49 +0800 Subject: [PATCH 015/132] clear space line metrics py --- bin/metrics/metrics.py | 3 --- 1 file changed, 3 deletions(-) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index d8c2bff0..218c7874 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -192,7 +192,6 @@ def pass_het_snps(mqc, biosample_id): return k, v - def pass_homalt_snps(mqc, biosample_id): """ The number of PASS hom alt SNPs. @@ -210,7 +209,6 @@ def pass_homalt_snps(mqc, biosample_id): return k, v - def pass_snp_het_hom(mqc, biosample_id): """ The het/hom ratio for PASS SNPs. @@ -228,7 +226,6 @@ def pass_snp_het_hom(mqc, biosample_id): return k, v - def pass_del(mqc, biosample_id): """ The number of PASS deletions. From 081d439fb95cac07cd4f033fdb1e1476bb78e722 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 5 Apr 2024 21:37:42 +0800 Subject: [PATCH 016/132] added count_variants.py --- bin/count_variants.py | 362 ++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 362 insertions(+) create mode 100644 bin/count_variants.py diff --git a/bin/count_variants.py b/bin/count_variants.py new file mode 100644 index 00000000..3acab70e --- /dev/null +++ b/bin/count_variants.py @@ -0,0 +1,362 @@ +#!/usr/bin/env python3 + +import logging +import argparse +import json +import pprint as pp +import subprocess +import numpy as np +import sys +import os +from pathlib import Path + + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--input_vcf", dest="input_vcf", required=True, + default=None, + help="Path to input VCF/gVCF.") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./variant_counts.json", + help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, + default="./", + help="Path to scratch dir. Default: ./") + parser.add_argument("--keep_scratch", dest="keep_scratch", required=False, + default=False, action="store_true", + help="Keep scratch dir. Default: False.") + parser.add_argument("--loglevel", dest="loglevel", required=False, + default="INFO", + help="Set logging level to INFO (default), WARNING or DEBUG.") + args = parser.parse_args() + + # create scratch dir if it doesn't exist + Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) + + return args + + +def set_logging(loglevel): + numeric_level = getattr(logging, loglevel.upper(), None) + if not isinstance(numeric_level, int): + raise ValueError('Invalid log level: %s' % loglevel) + logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', + level=numeric_level) + + +def setup(input_vcf, scratch_dir): + + # subset input vcf to include only variant sites to speed up execution from gvcf + logging.info("Subsetting variant sites from input vcf...") + variants_vcf = "%s/variants.vcf.gz" % scratch_dir + cmd = "bcftools view" + cmd += " -e 'GT=\"./.\" | GT=\".|.\" | GT=\".\" | GT=\"0/0\" | GT=\"0|0\" | GT=\"0\"'" + cmd += " -O z -o %s %s" % (variants_vcf, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + return variants_vcf + + +def count_variants(input_vcf, scratch_dir): + + # initialise results dict + r = dict() + metrics_list = [ + "all_snps", "all_het_snps", "all_homalt_snps", "all_snp_het_hom", + "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", + "all_indels", "all_het_indels", "all_homalt_indels", "all_indel_het_hom", + "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", + "all_del", "all_ins", "pass_del", "pass_ins", + "all_mnps", "pass_mnps", + "all_complex_indels", "all_complex_ins", "all_complex_del", + "pass_complex_indels", "pass_complex_ins", "pass_complex_del", + "all_multiallelic_sites", "all_multiallelic_sites", + "all_snp_ts_tv", "pass_snp_ts_tv" + ] + for m in metrics_list: + r[m] = 0 + + # run bcftools stats - used to obtain ts/tv and sanity checks + logging.info("Running bcftools stats for all variants...") + cmd = "bcftools stats %s > %s/all.bcftools_stats" % (input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + logging.info("Running bcftools stats for PASS variants...") + cmd = "bcftools stats -f PASS %s > %s/pass.bcftools_stats" % (input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + # calculate metrics + logging.info("Counting all_snps...") + cmd = "bcftools view -H -v snps %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_snps"] = int(p.stdout.read()) + + logging.info("Counting all_het_snps...") + cmd = "bcftools view -H -v snps -g het %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_het_snps"] = int(p.stdout.read()) + + logging.info("Counting all_homalt_snps...") + cmd = "bcftools view -H -v snps -g hom %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_homalt_snps"] = int(p.stdout.read()) + + logging.info("Calculating all_snp_het_hom...") + snp_het_hom = np.divide(r["all_het_snps"], r["all_homalt_snps"]) + r["all_snp_het_hom"] = np.round(snp_het_hom, 2) + + logging.info("Counting pass_snps...") + cmd = "bcftools view -H -v snps -f PASS %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_het_snps...") + cmd = "bcftools view -H -v snps -f PASS -g het %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_snps...") + cmd = "bcftools view -H -v snps -f PASS -g hom %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_snps"] = int(p.stdout.read()) + + logging.info("Calculating pass_snp_het_hom...") + snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) + r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) + + logging.info("Counting all_indels...") + cmd = "bcftools view -H -v indels %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_indels"] = int(p.stdout.read()) + + logging.info("Counting all_het_indels...") + cmd = "bcftools view -H -v indels -g het %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_het_indels"] = int(p.stdout.read()) + + logging.info("Counting all_homalt_indels...") + cmd = "bcftools view -H -v indels -g hom %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_homalt_indels"] = int(p.stdout.read()) + + logging.info("Calculating all_indel_het_hom...") + indel_het_hom = np.divide(r["all_het_indels"], r["all_homalt_indels"]) + r["all_indel_het_hom"] = np.round(indel_het_hom, 2) + + logging.info("Counting pass_indels...") + cmd = "bcftools view -H -v indels -f PASS %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_het_indels...") + cmd = "bcftools view -H -v indels -f PASS -g het %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_indels...") + cmd = "bcftools view -H -v indels -f PASS -g hom %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_indels"] = int(p.stdout.read()) + + logging.info("Calculating pass_indel_het_hom...") + indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) + r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) + + logging.info("Counting all_del, all_ins...") + cmd = "bcftools view -H -v indels %s" % input_vcf + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + # if length(ALT) < length(REF), label as DEL + cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "all_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Counting pass_del, pass_ins...") + cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + # if length(ALT) < length(REF), label as DEL + cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Counting all_mnps...") + cmd = "bcftools view -H -v mnps %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_mnps"] = int(p.stdout.read()) + + logging.info("Counting pass_mnps...") + cmd = "bcftools view -H -v mnps -f PASS %s | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_mnps"] = int(p.stdout.read()) + + logging.info("Counting all_complex_indels...") + cmd = "bcftools view -H -v indels %s" % input_vcf + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF) and length(REF) > 1, label as COMPLEX_INS + cmd += " | awk '{ if( length($5) > length($4) && length($4) > 1 ) { print \"COMPLEX_INS\" }" + # if length(ALT) < length(REF) and length(ALT) > 1, label as COMPLEX_DEL + cmd += " else if ( length($5) < length($4) && length($5) > 1 ) { print \"COMPLEX_DEL\" } }'" + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "all_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + r["all_complex_indels"] = r["all_complex_ins"] + r["all_complex_del"] + + logging.info("Counting pass_complex_indels...") + cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF) and length(REF) > 1, label as COMPLEX_INS + cmd += " | awk '{ if( length($5) > length($4) && length($4) > 1 ) { print \"COMPLEX_INS\" }" + # if length(ALT) < length(REF) and length(ALT) > 1, label as COMPLEX_DEL + cmd += " else if ( length($5) < length($4) && length($5) > 1 ) { print \"COMPLEX_DEL\" } }'" + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + r["pass_complex_indels"] = r["pass_complex_ins"] + r["pass_complex_del"] + + logging.info("Calculating all_multiallelic_sites...") + cmd = "bcftools view -H %s | sed 's/,//g' | awk '$5 ~ /,/' | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_multiallelic_sites"] = int(p.stdout.read()) + + logging.info("Calculating pass_multiallelic_sites...") + cmd = "bcftools view -H -f PASS %s | sed 's/,//g' | awk '$5 ~ /,/' | wc -l" % input_vcf + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_multiallelic_sites"] = int(p.stdout.read()) + + logging.info("Calculating all_snp_ts_tv...") + cmd = "cat %s/all.bcftools_stats | grep '^TSTV'" % scratch_dir + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + l = p.stdout.read().decode("utf-8").strip().split("\t") + r["all_snp_ts_tv"] = np.round(float(l[4]), 2) + + logging.info("Calculating pass_snp_ts_tv...") + cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + l = p.stdout.read().decode("utf-8").strip().split("\t") + r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) + + # sanity checks + logging.info("Running sanity checks for variant counts...") + + error_count = 0 + cmd = "cat %s/all.bcftools_stats | grep '^SN'" % scratch_dir + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + + for l in p.stdout.read().splitlines(): + l = l.decode("utf-8").strip().split("\t") + k = str(l[2]).lower() + v = int(l[3]) + if "snps" in k and v != r["all_snps"]: + logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) + error_count += 1 + elif "indels" in k and v != r["all_indels"]: + logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) + error_count += 1 + elif "mnps" in k and v != r["all_mnps"]: + logging.error("Mismatch in MNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_mnps"])) + error_count += 1 + + if error_count > 0: + sys.exit("Sanity checks failed, aborting execution") + + return r + + +def save_output(d, outfile): + with open(outfile, "w") as f: + json.dump(d, f, sort_keys=True, indent=4) + f.write("\n") + + +def done(scratch_dir): + if not args.keep_scratch: + # clean up + logging.info("Cleaning up...") + os.remove("%s/variants.vcf.gz" % scratch_dir) + os.remove("%s/all.bcftools_stats" % scratch_dir) + os.remove("%s/pass.bcftools_stats" % scratch_dir) + if len(os.listdir(scratch_dir)) == 0: + os.rmdir(scratch_dir) + else: + logging.warning("Couldn't remove scratch dir (%s): directory is not empty" % scratch_dir) + logging.info("DONE") + + +if __name__ == "__main__": + args = parse_args() + set_logging(args.loglevel) + + variants_vcf = setup(args.input_vcf, args.scratch_dir) + + vcf_metrics = count_variants(variants_vcf, args.scratch_dir) + save_output(vcf_metrics, args.output_json) + + done(args.scratch_dir) From c201f5f28da9032fc012edced4e6b01c0c481c55 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 07:51:03 +0800 Subject: [PATCH 017/132] add arg regions to count_variants py --- bin/count_variants.py | 3 +++ 1 file changed, 3 insertions(+) diff --git a/bin/count_variants.py b/bin/count_variants.py index 3acab70e..8715dbd8 100644 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -16,6 +16,9 @@ def parse_args(): parser.add_argument("--input_vcf", dest="input_vcf", required=True, default=None, help="Path to input VCF/gVCF.") + parser.add_argument("--regions", dest="regions", required=True, + default=None, + help="Path to input autosomes_non_gap_regions_bed.") parser.add_argument("--output_json", dest="output_json", required=False, default="./variant_counts.json", help="Path to output file for variant metrics. Default: ./variant_counts.json") From c8d98aad03dfd02dfd3b5dedb1b2d6f276d6d4c2 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 08:17:53 +0800 Subject: [PATCH 018/132] autosome regions as channel in to count_variants py --- modules/count_variants/main.nf | 5 +++-- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml | 3 --- tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 2 +- 3 files changed, 4 insertions(+), 6 deletions(-) diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index 61e0ba01..6027c320 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -3,7 +3,8 @@ process count_variants { tag { sample } input: - tuple val(sample), path(vcf), path(tbi), path(autosomes_non_gap_regions) + tuple val(sample), path(vcf), path(tbi) + path(autosomes_non_gap_regions) output: tuple val(sample), path("${sample}.variant_counts.json") @@ -18,4 +19,4 @@ process count_variants { --loglevel DEBUG """ -} \ No newline at end of file +} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml index 034fec6b..dd73cf16 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml @@ -1,7 +1,4 @@ samples: -- - biosample_id: NA12877 - aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12877/NA12877.bam - biosample_id: NA12878 aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 7ab59f8a..49dd4b8e 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -6,7 +6,7 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -nextflow run ../../main.nf \ +/Users/gnanakkan/bin/nf-23.09.1-edge/nextflow run ../../main.nf \ -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ From 659548df0db8f599998e5e003d574d0f00c35dce Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 08:21:01 +0800 Subject: [PATCH 019/132] test changes only main.nf, nextflow.config, param.yaml --- main.nf | 8 ++++---- nextflow.config | 4 ++-- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml | 1 + 3 files changed, 7 insertions(+), 6 deletions(-) diff --git a/main.nf b/main.nf index 1b868425..ceb645b0 100644 --- a/main.nf +++ b/main.nf @@ -140,14 +140,14 @@ workflow { .set { aln_inputs } - samtools_stats_bam( aln_inputs.bam, [] ) - samtools_stats_cram( aln_inputs.cram, ref_fasta ) + //samtools_stats_bam( aln_inputs.bam, [] ) + //samtools_stats_cram( aln_inputs.cram, ref_fasta ) verifybamid2_bam( aln_inputs.bam, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) verifybamid2_cram( aln_inputs.cram, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) - picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) - picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) + //picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) + //picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) //picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) //picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) diff --git a/nextflow.config b/nextflow.config index 818903d1..ece95a03 100644 --- a/nextflow.config +++ b/nextflow.config @@ -50,7 +50,7 @@ process { withName: 'verifybamid2' { cpus = 4 - disk = '50 GB' + disk = '5 GB' memory = { 2.GB * task.attempt } publishDir = [ @@ -115,7 +115,7 @@ process { withName: 'multiqc' { cpus = 1 - disk = '50 GB' + disk = '5 GB' memory = { 2.GB * task.attempt } publishDir = [ diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml index dd73cf16..f55939d8 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml @@ -2,4 +2,5 @@ samples: - biosample_id: NA12878 aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam + vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz aws_no_sign_request: true From a7080a0f160842cbb9edd6f9ae1036d13f7a4180 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 11:17:44 +0800 Subject: [PATCH 020/132] include bcftools conda_env.yml --- containers/conda_env.yml | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/containers/conda_env.yml b/containers/conda_env.yml index 0050a1c7..34fdedb2 100644 --- a/containers/conda_env.yml +++ b/containers/conda_env.yml @@ -3,9 +3,11 @@ channels: - bioconda - conda-forge - defaults +channel_priority: strict dependencies: - - python=3.7 + - python=3.11 - samtools=1.15 + - bcftools=1.19 - picard=2.27.0 - bedtools=2.29.2 - mosdepth=0.3.3 From 6fb4cf3fef1affbdabae5d5e04f67397c330eba0 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 11:19:15 +0800 Subject: [PATCH 021/132] change docker image version v0.12.0 --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index ece95a03..7da82e18 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,7 +9,7 @@ manifest { version = '0.12.0' } -docker_version = '0.11.0' +docker_version = '0.12.0' cleanup = true From 27861a50a80cd261e59ff9cd77d2ec1eda9749ae Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 11:19:34 +0800 Subject: [PATCH 022/132] bcftools added to image --- containers/Dockerfile.BAK | 75 +++++++++++++++++++++++++++++++++++++++ 1 file changed, 75 insertions(+) create mode 100644 containers/Dockerfile.BAK diff --git a/containers/Dockerfile.BAK b/containers/Dockerfile.BAK new file mode 100644 index 00000000..565121c9 --- /dev/null +++ b/containers/Dockerfile.BAK @@ -0,0 +1,75 @@ +FROM ubuntu:22.04 + +LABEL base_image="ubuntu:22.04" + +ENV LC_ALL=C.UTF-8 +ENV LANG=C.UTF-8 + +ENV PATH="/usr/local/conda/envs/npm-sample-qc/bin/:${PATH}" +ENV PATH="/usr/local/conda/envs/npm-sample-qc/share/picard-2.27.0-0/:${PATH}" +ENV PATH="/usr/local/conda/envs/npm-sample-qc/share/verifybamid2-2.0.1-7/:${PATH}" +ENV PATH="/usr/local/conda/bin/:${PATH}" + +ADD conda_env.yml /conda_env.yml + +RUN export DEBIAN_FRONTEND="noninteractive" && \ + apt-get update && \ + apt-get install -y --no-install-recommends \ + build-essential \ + libtool \ + libarchive-dev \ + make cmake m4 automake \ + gcc g++ \ + python3 python-pip python-dev-is-python3 \ + git \ + git-lfs \ + wget \ + zlib1g-dev \ + libbz2-dev \ + liblzma-dev \ + libmath-libm-perl \ + libpthread-stubs0-dev \ + curl \ + python3-pycurl \ + libncurses-dev \ + libboost-all-dev \ + libgtest-dev \ + cmake \ + libssl-dev \ + libcurl3-dev \ + unzip \ + gnuplot \ + datamash \ + graphviz \ + && rm -rf /var/lib/apt/lists/* + +RUN wget --max-redirect=1 https://github.com/samtools/bcftools/releases/download/1.19/bcftools-1.19.tar.bz2 \ + && tar -xf bcftools-1.19.tar.bz2 \ + && mkdir bcftools \ + && cd bcftools-1.19 \ + && ./configure --prefix=/bcftools \ + && make \ + && make install \ + && cd ../ \ + && rm bcftools-1.19.tar.bz2 \ + && apt-get install -y tabix + +ENV PATH="$PATH:/bcftools/bin" + + # install conda +RUN wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh +RUN bash Miniconda3-latest-Linux-x86_64.sh -b -p /usr/local/conda +RUN PATH="/usr/local/conda/bin:${PATH}" +RUN rm Miniconda3-latest-Linux-x86_64.sh +RUN conda update -n base -c defaults conda + +RUN conda install -c conda-forge -y awscli + + # create conda environment from template +RUN conda env create -f /conda_env.yml +RUN conda clean -a + + # install MultiQC and MultiQC_NPM plugin +RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git +WORKDIR /NPM-sample-qc/bin/multiqc_plugins +RUN python3 setup.py install From 2829052909b704339742975e182e5438b232bb3b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 12:31:27 +0800 Subject: [PATCH 023/132] add sample_id to combine the channel for multiqc --- main.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index ceb645b0..33b5c38d 100644 --- a/main.nf +++ b/main.nf @@ -162,13 +162,15 @@ workflow { return tuple( rec.biosample_id, vcf_file, vcf_idx ) } - .view() .set { vcf_inputs } bcftools_stats( vcf_inputs ) //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) + Channel + samples.map { it.biosample_id } + .set { sample_ids } /* // channel for samplelist input file type bam processed outputs From 89d9df7d524deac9d46bf3363343d13d4646af37 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 23:06:07 +0800 Subject: [PATCH 024/132] added npm_count_variants.py --- .../multiqc_npm/modules/npm_count_variants.py | 34 +++++++++++++++++++ 1 file changed, 34 insertions(+) create mode 100644 bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py b/bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py new file mode 100644 index 00000000..49ccf96f --- /dev/null +++ b/bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py @@ -0,0 +1,34 @@ +#!/usr/bin/env python3 + +""" +Parser for count_variants.py +""" + +import logging +import json + +from multiqc.utils import report + +log = logging.getLogger(__name__) + + +def parse_reports(self): + + # Set up vars + self.count_variants = dict() + + # Collect metrics + for f in self.find_log_files('multiqc_npm/count_variants'): + parsed_data = json.loads(f["f"]) + # Save results + s_name = f["s_name"] + self.count_variants[s_name] = parsed_data + + # Write results + if len(self.count_variants) > 0: + + # Write parsed data to a file + self.write_data_file(self.count_variants, 'multiqc_npm_count_variants') + + # Return the number of detected samples to the parent module + return len(self.count_variants) From a51fc0abcb6aac2a967508fbd539106a5fe4b11f Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 23:09:09 +0800 Subject: [PATCH 025/132] update npm_extra_metrics.py --- bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py b/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py index 140d5c32..7a84be68 100644 --- a/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py +++ b/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py @@ -12,6 +12,7 @@ from . import npm_picard_QualityYieldMetrics from . import npm_samtools_stats_bq +from . import npm_count_variants from . import npm_mosdepth log = logging.getLogger('multiqc') @@ -46,6 +47,10 @@ def __init__(self): if n['npm_samtools_stats_bq'] > 0: log.info("Found %d npm_samtools_stats_bq reports" % n['npm_samtools_stats_bq']) + n['npm_count_variants'] = npm_count_variants.parse_reports(self) + if n['npm_count_variants'] > 0: + log.info("Found %d npm_count_variants reports" % n['npm_count_variants']) + n['npm_mosdepth'] = npm_mosdepth.parse_reports(self) if n['npm_mosdepth'] > 0: log.info("Found %d npm_mosdepth reports" % n['npm_mosdepth']) From 4aa69a77bf60273c19676c9ec83c0296d679b369 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 23:20:34 +0800 Subject: [PATCH 026/132] update hooks.py --- bin/multiqc_plugins/multiqc_npm/hooks.py | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/bin/multiqc_plugins/multiqc_npm/hooks.py b/bin/multiqc_plugins/multiqc_npm/hooks.py index c2c9a7c7..d47965d7 100644 --- a/bin/multiqc_plugins/multiqc_npm/hooks.py +++ b/bin/multiqc_plugins/multiqc_npm/hooks.py @@ -19,7 +19,8 @@ def before_config(): my_search_patterns = { 'multiqc_npm/picard_quality_yield_metrics': {'fn': '*.quality_yield_metrics.txt', 'shared': 'true'}, - 'multiqc_npm/samtools_stats_bq': {'fn': '*.stats', 'contents': 'This file was produced by samtools stats', 'shared': 'true'} + 'multiqc_npm/samtools_stats_bq': {'fn': '*.stats', 'contents': 'This file was produced by samtools stats', 'shared': 'true'}, + 'multiqc_npm/count_variants': {'fn': '*.variant_counts.json'} } config.update_dict(config.sp, my_search_patterns) log.info("Expanded search patterns with the following: %s", ", ".join(my_search_patterns.keys())) From 6253e6714f1ba9f3f56e4ad3cd5f3cf48444ea23 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sat, 6 Apr 2024 23:37:06 +0800 Subject: [PATCH 027/132] changes to test count_variants.py --- containers/conda_env.yml | 5 ++--- nextflow.config | 6 +++--- tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 1 + 3 files changed, 6 insertions(+), 6 deletions(-) diff --git a/containers/conda_env.yml b/containers/conda_env.yml index 34fdedb2..4a7fd0fd 100644 --- a/containers/conda_env.yml +++ b/containers/conda_env.yml @@ -3,11 +3,10 @@ channels: - bioconda - conda-forge - defaults -channel_priority: strict dependencies: - - python=3.11 + - python=3.7 - samtools=1.15 - - bcftools=1.19 + - bcftools=1.15 - picard=2.27.0 - bedtools=2.29.2 - mosdepth=0.3.3 diff --git a/nextflow.config b/nextflow.config index 7da82e18..3551388a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -151,9 +151,9 @@ docker.tmp = "auto" // To use container provisioning service integrated with Nextflow // See https://www.nextflow.io/docs/latest/wave.html -wave { - enabled = true -} +// wave { +// enabled = true +// } // Load resources.config by default diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 49dd4b8e..5493cf10 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -10,4 +10,5 @@ NXF_ANSI_LOG=false -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ + -resume \ --publish_dir ./output From fa2de9d3a6e52e4abb5d805ca69ed2822f745d7d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 09:49:16 +0800 Subject: [PATCH 028/132] revert to wave enable --- modules/count_variants/main.nf | 1 - nextflow.config | 6 +++--- 2 files changed, 3 insertions(+), 4 deletions(-) diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index 6027c320..bb0dc193 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -11,7 +11,6 @@ process count_variants { script: """ - # echo "test vcf module" ${vcf} ${tbi} >${sample}.variant_counts.json count_variants.py \ --input_vcf ${vcf} \ --regions ${autosomes_non_gap_regions} \ diff --git a/nextflow.config b/nextflow.config index 3551388a..7da82e18 100644 --- a/nextflow.config +++ b/nextflow.config @@ -151,9 +151,9 @@ docker.tmp = "auto" // To use container provisioning service integrated with Nextflow // See https://www.nextflow.io/docs/latest/wave.html -// wave { -// enabled = true -// } +wave { + enabled = true +} // Load resources.config by default From e9a8505739a22da5bc5c6c8e7d9b9bf73bb5369b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 11:46:09 +0800 Subject: [PATCH 029/132] count_varinats py codes update on various files and folders --- bin/compile_metrics.py | 1 + bin/metrics/metrics.py | 17 +++++++++++++++++ main.nf | 14 ++++++-------- nextflow.config | 10 +++++----- 4 files changed, 29 insertions(+), 13 deletions(-) diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index 9e7221cf..f4591736 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -56,6 +56,7 @@ def calculate_metrics(mqc): "cross_contamination_rate" # variant calling "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", + "pass_indel_het_hom", "pass_del", "pass_ins", "pass_snp_ts_tv" ] diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index 218c7874..6b0b94d9 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -226,6 +226,23 @@ def pass_snp_het_hom(mqc, biosample_id): return k, v +def pass_indel_het_hom(mqc, biosample_id): + """ + The het/hom ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id] + v = d["pass_indel_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + def pass_del(mqc, biosample_id): """ The number of PASS deletions. diff --git a/main.nf b/main.nf index 33b5c38d..cd71ac4d 100644 --- a/main.nf +++ b/main.nf @@ -140,17 +140,17 @@ workflow { .set { aln_inputs } - //samtools_stats_bam( aln_inputs.bam, [] ) - //samtools_stats_cram( aln_inputs.cram, ref_fasta ) + samtools_stats_bam( aln_inputs.bam, [] ) + samtools_stats_cram( aln_inputs.cram, ref_fasta ) verifybamid2_bam( aln_inputs.bam, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) verifybamid2_cram( aln_inputs.cram, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) - //picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) - //picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) + picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) + picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) - //picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - //picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) Channel @@ -172,7 +172,6 @@ workflow { samples.map { it.biosample_id } .set { sample_ids } -/* // channel for samplelist input file type bam processed outputs Channel .empty() @@ -200,7 +199,6 @@ workflow { multiqc( multiqc_in ) -*/ } /* diff --git a/nextflow.config b/nextflow.config index 7da82e18..d014374a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -50,7 +50,7 @@ process { withName: 'verifybamid2' { cpus = 4 - disk = '5 GB' + disk = '50 GB' memory = { 2.GB * task.attempt } publishDir = [ @@ -83,7 +83,7 @@ process { withName:bcftools_stats { cpus = 4 - disk = '1 GB' + disk = '50 GB' memory = '1 GB' publishDir = [ @@ -94,7 +94,7 @@ process { withName:picard_collect_variant_calling_metrics_vcf { cpus = 4 - disk = '1 GB' + disk = '50 GB' memory = '2 GB' publishDir = [ @@ -103,8 +103,8 @@ process { ] } withName:count_variants { - cpus = 4 - disk = '1 GB' + cpus = 16 + disk = '50 GB' memory = '1 GB' publishDir = [ From 457e8895d11fd5bbb57900ecb513fdb97c78f01b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 10:40:31 +0000 Subject: [PATCH 030/132] permission +x count_variants.py and resource update nextflow.config --- bin/count_variants.py | 0 1 file changed, 0 insertions(+), 0 deletions(-) mode change 100644 => 100755 bin/count_variants.py diff --git a/bin/count_variants.py b/bin/count_variants.py old mode 100644 new mode 100755 From 31c64969ac32ba3b7c53eb99b433a0cef6db934c Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 13:22:10 +0000 Subject: [PATCH 031/132] tested all the process except multiqc --- main.nf | 24 +++++++++++++++++++----- modules/multiqc/main.nf | 3 ++- nextflow.config | 4 ++-- 3 files changed, 23 insertions(+), 8 deletions(-) diff --git a/main.nf b/main.nf index cd71ac4d..296e0231 100644 --- a/main.nf +++ b/main.nf @@ -149,8 +149,8 @@ workflow { picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) - picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + //picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + //picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) Channel @@ -172,13 +172,23 @@ workflow { samples.map { it.biosample_id } .set { sample_ids } +// channel for samplelist vcf input file processed outputs + Channel + .empty() + //sample_ids + //.join( count_variants.out ) + count_variants.out + .join( bcftools_stats.out ) + .view() + .set { vcf_qc } + // channel for samplelist input file type bam processed outputs Channel .empty() samtools_stats_bam.out.stats .join( picard_collect_multiple_metrics_bam.out.insert_size ) .join( picard_collect_multiple_metrics_bam.out.quality ) - .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) + //.join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) .join( verifybamid2_bam.out.freemix, remainder: true ) .set { ch_bam } @@ -188,19 +198,23 @@ workflow { samtools_stats_cram.out.stats .join( picard_collect_multiple_metrics_cram.out.insert_size ) .join( picard_collect_multiple_metrics_cram.out.quality ) - .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) + //.join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) .join( verifybamid2_cram.out.freemix, remainder: true ) .set { ch_cram } // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty ch_bam.mix(ch_cram) - .map { sample, stats, insertsize, quality, wgs_coverage, freemix -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [] ] } + .combine(vcf_qc,by:0) + .map { sample, stats, insertsize, quality, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, freemix ?: [], count_variants, bcftools_stats ] } + .view() .set { multiqc_in } multiqc( multiqc_in ) } +// .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } + /* ---------------------------------------------------------------------- COMPLETION INFO diff --git a/modules/multiqc/main.nf b/modules/multiqc/main.nf index 6eebb087..64e38f19 100644 --- a/modules/multiqc/main.nf +++ b/modules/multiqc/main.nf @@ -3,7 +3,8 @@ process multiqc { tag { sample } input: - tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix) + // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) + tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) output: tuple val(sample), path("${sample}/multiqc_report.html"), emit: report diff --git a/nextflow.config b/nextflow.config index d014374a..332b1862 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,7 +11,7 @@ manifest { docker_version = '0.12.0' -cleanup = true +// cleanup = true params { @@ -115,7 +115,7 @@ process { withName: 'multiqc' { cpus = 1 - disk = '5 GB' + disk = '50 GB' memory = { 2.GB * task.attempt } publishDir = [ From 93800ad17b988e723d80d6b6056c1cb853dda2a4 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 13:22:46 +0000 Subject: [PATCH 032/132] tested all the process except multiqc --- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml index f55939d8..2d0e3cb4 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml @@ -1,6 +1,5 @@ samples: - biosample_id: NA12878 - aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam - vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz -aws_no_sign_request: true + aln: /data/NA12878.bam + vcf: /data/NA12878.hard-filtered.vcf.gz From 70780e71e426da5124f0e96c6774f45f094f440e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 7 Apr 2024 13:23:07 +0000 Subject: [PATCH 033/132] tested all the process except multiqc --- tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 5493cf10..68ac94d1 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -6,9 +6,9 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/Users/gnanakkan/bin/nf-23.09.1-edge/nextflow run ../../main.nf \ +/home/ubuntu/bin/nf23.09.1-edge-all/nextflow run ../../main.nf \ -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ - -resume \ + -resume \ --publish_dir ./output From ab849feec4930e975b6420b0e93b456b36ede8a5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 8 Apr 2024 00:42:10 +0000 Subject: [PATCH 034/132] remove import mpsdepth in npm_extra_metrics.py --- bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py | 5 ----- 1 file changed, 5 deletions(-) diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py b/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py index 7a84be68..a6516cf7 100644 --- a/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py +++ b/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py @@ -13,7 +13,6 @@ from . import npm_picard_QualityYieldMetrics from . import npm_samtools_stats_bq from . import npm_count_variants -from . import npm_mosdepth log = logging.getLogger('multiqc') @@ -51,10 +50,6 @@ def __init__(self): if n['npm_count_variants'] > 0: log.info("Found %d npm_count_variants reports" % n['npm_count_variants']) - n['npm_mosdepth'] = npm_mosdepth.parse_reports(self) - if n['npm_mosdepth'] > 0: - log.info("Found %d npm_mosdepth reports" % n['npm_mosdepth']) - # Exit if we didn't find anything if sum(n.values()) == 0: raise UserWarning From 746013f8f54df8c751a3e8dd27d785787df62717 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 8 Apr 2024 06:32:47 +0000 Subject: [PATCH 035/132] add git checkout vcf_qc_implementation --- containers/Dockerfile | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/containers/Dockerfile b/containers/Dockerfile index 9052cd65..a8acd9a9 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -45,5 +45,11 @@ RUN conda clean -a # install MultiQC and MultiQC_NPM plugin RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git + +#RUN git clone -b feature/vcf-qc-implementation --single-branch https://github.com/c-BIG/NPM-sample-qc.git + WORKDIR /NPM-sample-qc/bin/multiqc_plugins + +RUN git checkout feature/vcf-qc-implementation RUN python3 setup.py install +# RUN python -m pip install . From a8e2c5e64c70cc424edcbd1c44c716a24873b90e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 8 Apr 2024 08:31:51 +0000 Subject: [PATCH 036/132] metrics.py update to fetch vcf qc values from multiqc_data.json --- bin/compile_metrics.py | 2 +- bin/metrics/metrics.py | 16 ++++++++-------- containers/Dockerfile | 5 +++-- main.nf | 6 +++--- 4 files changed, 15 insertions(+), 14 deletions(-) diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index f4591736..460c2740 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -53,7 +53,7 @@ def calculate_metrics(mqc): # insert size "mean_insert_size", "insert_size_std_deviation", - "cross_contamination_rate" + "cross_contamination_rate", # variant calling "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", "pass_indel_het_hom", diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index 6b0b94d9..c123dfc1 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -167,7 +167,7 @@ def pass_snps(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_snps"] v = int(v) except KeyError: @@ -184,7 +184,7 @@ def pass_het_snps(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_het_snps"] v = int(v) except KeyError: @@ -201,7 +201,7 @@ def pass_homalt_snps(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_homalt_snps"] v = int(v) except KeyError: @@ -218,7 +218,7 @@ def pass_snp_het_hom(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_snp_het_hom"] v = np.round(v, DECIMALS) except KeyError: @@ -235,7 +235,7 @@ def pass_indel_het_hom(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_indel_het_hom"] v = np.round(v, DECIMALS) except KeyError: @@ -252,7 +252,7 @@ def pass_del(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_del"] v = int(v) except KeyError: @@ -269,7 +269,7 @@ def pass_ins(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_ins"] v = int(v) except KeyError: @@ -286,7 +286,7 @@ def pass_snp_ts_tv(mqc, biosample_id): k = inspect.currentframe().f_code.co_name try: - d = mqc["multiqc_npm_count_variants"][biosample_id] + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] v = d["pass_snp_ts_tv"] v = np.round(v, DECIMALS) except KeyError: diff --git a/containers/Dockerfile b/containers/Dockerfile index a8acd9a9..c4b27c92 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -44,12 +44,13 @@ RUN conda env create -f /conda_env.yml RUN conda clean -a # install MultiQC and MultiQC_NPM plugin -RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git +# RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git #RUN git clone -b feature/vcf-qc-implementation --single-branch https://github.com/c-BIG/NPM-sample-qc.git - +RUN git clone https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin/multiqc_plugins RUN git checkout feature/vcf-qc-implementation RUN python3 setup.py install # RUN python -m pip install . +# RUN pip3 install numpy scipy argparse matplotlib diff --git a/main.nf b/main.nf index 296e0231..e95bef1a 100644 --- a/main.nf +++ b/main.nf @@ -188,7 +188,7 @@ workflow { samtools_stats_bam.out.stats .join( picard_collect_multiple_metrics_bam.out.insert_size ) .join( picard_collect_multiple_metrics_bam.out.quality ) - //.join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) + .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) .join( verifybamid2_bam.out.freemix, remainder: true ) .set { ch_bam } @@ -198,14 +198,14 @@ workflow { samtools_stats_cram.out.stats .join( picard_collect_multiple_metrics_cram.out.insert_size ) .join( picard_collect_multiple_metrics_cram.out.quality ) - //.join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) + .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) .join( verifybamid2_cram.out.freemix, remainder: true ) .set { ch_cram } // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty ch_bam.mix(ch_cram) .combine(vcf_qc,by:0) - .map { sample, stats, insertsize, quality, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, freemix ?: [], count_variants, bcftools_stats ] } + .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } .view() .set { multiqc_in } From 7884bd97db606ba382e7ba3876412f19c72eae0c Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 8 Apr 2024 08:33:59 +0000 Subject: [PATCH 037/132] update multiqc main.nf picard wgs metrics input tuple --- modules/multiqc/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/multiqc/main.nf b/modules/multiqc/main.nf index 64e38f19..73effba5 100644 --- a/modules/multiqc/main.nf +++ b/modules/multiqc/main.nf @@ -3,8 +3,8 @@ process multiqc { tag { sample } input: - // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) - tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) + tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) + // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) output: tuple val(sample), path("${sample}/multiqc_report.html"), emit: report From 8af29aba7eba14f8a3fd22731cc36422acf9fb7d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 8 Apr 2024 08:35:41 +0000 Subject: [PATCH 038/132] uncomment picard wgs metrics process --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index e95bef1a..0a501ea4 100644 --- a/main.nf +++ b/main.nf @@ -149,8 +149,8 @@ workflow { picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) - //picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - //picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) Channel From 4d484ffb8f190518a0eb8c3dfbb23aa5b4339212 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 14 May 2024 04:38:31 +0000 Subject: [PATCH 039/132] process varying number of processed outputs from aln or vcf or aln-vcf optional input to multiqc channel --- main.nf | 53 ++++++++++++++++++++++++++++++++++++----- modules/multiqc/main.nf | 4 +++- 2 files changed, 50 insertions(+), 7 deletions(-) diff --git a/main.nf b/main.nf index 0a501ea4..927ce7a2 100644 --- a/main.nf +++ b/main.nf @@ -175,17 +175,18 @@ workflow { // channel for samplelist vcf input file processed outputs Channel .empty() - //sample_ids - //.join( count_variants.out ) - count_variants.out + sample_ids + .join( count_variants.out ) + //count_variants.out .join( bcftools_stats.out ) - .view() + //.view() .set { vcf_qc } // channel for samplelist input file type bam processed outputs Channel .empty() - samtools_stats_bam.out.stats + sample_ids + .join( samtools_stats_bam.out.stats ) .join( picard_collect_multiple_metrics_bam.out.insert_size ) .join( picard_collect_multiple_metrics_bam.out.quality ) .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) @@ -195,19 +196,59 @@ workflow { // channel for samplelist input file type cram processed outputs Channel .empty() - samtools_stats_cram.out.stats + sample_ids + .join( samtools_stats_cram.out.stats ) .join( picard_collect_multiple_metrics_cram.out.insert_size ) .join( picard_collect_multiple_metrics_cram.out.quality ) .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) .join( verifybamid2_cram.out.freemix, remainder: true ) .set { ch_cram } +/* // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty ch_bam.mix(ch_cram) .combine(vcf_qc,by:0) + //.view() .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } + //.view() + .set { multiqc_in } +*/ +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + ch_bam.mix(ch_cram) // .ifEmpty([]) + //.combine(vcf_qc,by:0) + .join(vcf_qc, remainder: true) + //.map { it.minus(null) } + .map { files -> files - null } + //.flatten() + //.map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } + .view() + .set { multiqc_in } + + +/* +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + Channel + .empty() + sample_ids + //.combine(vcf_qc,by:0).ifEmpty([]) + //ch_bam.mix(ch_cram).ifEmpty([]) + .join(count_variants.out) + .join(bcftools_stats.out) + .map { sample, count_variants, bcftools_stats -> sample, count_variants, bcftools_stats } + .mix(ch_bam,ch_cram) + //.combine(vcf_qc,by:0).ifEmpty([]) + //.join(vcf_qc) + //.view() + //.mix(vcf_qc).ifEmpty([]) + //.join(vcf_qc).ifEmpty([]) + //.combine(vcf_qc,by:0).ifEmpty([]) + //.flatten() + //.toList() .view() + //.map { sample, count_variants, bcftools_stats, sample1, stats, insertsize, quality, wgs_coverage, freemix -> [ sample ?: [], count_variants ?: [], bcftools_stats ?: [], sample1 ?: [], stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [] ] } + //.view() .set { multiqc_in } +*/ multiqc( multiqc_in ) diff --git a/modules/multiqc/main.nf b/modules/multiqc/main.nf index 73effba5..758b7644 100644 --- a/modules/multiqc/main.nf +++ b/modules/multiqc/main.nf @@ -3,7 +3,9 @@ process multiqc { tag { sample } input: - tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) + //tuple val(sample), path(variantscounts), path(stats) + tuple val(sample), path('*') + // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) output: From 20f8e40837814df1d28b37ba2e8ae51869295573 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 27 May 2024 04:32:27 +0000 Subject: [PATCH 040/132] unittest new vcf region of NA12878-AKT1-chr14-101769349-106795748 --- .../NA12878-chr14-AKT1.vcf | Bin 9 -> 609662 bytes .../NA12878-chr14-AKT1.vcf.tbi | Bin 9 -> 3298 bytes .../readme.txt | 6 ++++++ 3 files changed, 6 insertions(+) create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/readme.txt diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf index 5d14d0004cb1a7cdf5d721fa5e64367be29d9cc0..82cf8fa36919e9efdb93b22140182ed81c03afdb 100644 GIT binary patch literal 609662 zcmZs>by$?$7dARWcS<*cbV$k|G6M)m4idtkC{jZS($XM}gn&Z`IP`#&z|bg&N=Y|J 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deletions(-) delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.tbi diff --git a/modules/multiqc/main.nf b/modules/multiqc/main.nf index 758b7644..2143f80b 100644 --- a/modules/multiqc/main.nf +++ b/modules/multiqc/main.nf @@ -3,8 +3,8 @@ process multiqc { tag { sample } input: - //tuple val(sample), path(variantscounts), path(stats) - tuple val(sample), path('*') + tuple val(sample), path(variantscounts), path(stats) + // tuple val(sample), path('*') // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) diff --git 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NA12878-chr14-AKT1.vcf + vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh index ad972d04..610fa8ff 100755 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh @@ -6,7 +6,7 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/Users/gnanakkan/bin/nf-23.09.1-edge/nextflow run ../../main.nf \ +nextflow run ../../main.nf \ -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config \ --inputs_list params.yaml \ -work-dir ./work \ From b12633fece39b3bec622f352202364f29be2b8ff Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 18 Jun 2024 08:58:14 +0000 Subject: [PATCH 046/132] update nextflow vesrion 24.04.2 --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 332b1862..2cdec64e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -5,7 +5,7 @@ manifest { homePage = 'https://github.com/c-BIG/NPM-sample-qc' description = 'Nextflow NPM-sample-qc analysis pipeline, part of the GHIF community.' mainScript = 'main.nf' - nextflowVersion = '!>=23.08.1-edge' + nextflowVersion = '!>=24.04.2' version = '0.12.0' } From c300e336d7e9716591400e74fb0c87bcae1b2f4e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 18 Jun 2024 09:41:39 +0000 Subject: [PATCH 047/132] update nextflow vesrion 22.10.1 --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 2cdec64e..a149ae9a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -5,7 +5,7 @@ manifest { homePage = 'https://github.com/c-BIG/NPM-sample-qc' description = 'Nextflow NPM-sample-qc analysis pipeline, part of the GHIF community.' mainScript = 'main.nf' - nextflowVersion = '!>=24.04.2' + nextflowVersion = '!>=23.10.1' version = '0.12.0' } From fdfdf87f4423e95abb7bbf3770f58c98af41cd28 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 19 Jun 2024 02:49:35 +0000 Subject: [PATCH 048/132] multiqc update to process aln and vcf --- main.nf | 4 ++-- modules/multiqc/main.nf | 5 ++--- 2 files changed, 4 insertions(+), 5 deletions(-) diff --git a/main.nf b/main.nf index 927ce7a2..81dd44e0 100644 --- a/main.nf +++ b/main.nf @@ -218,9 +218,9 @@ workflow { //.combine(vcf_qc,by:0) .join(vcf_qc, remainder: true) //.map { it.minus(null) } - .map { files -> files - null } + //.map { files -> files - null } //.flatten() - //.map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } + .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } .view() .set { multiqc_in } diff --git a/modules/multiqc/main.nf b/modules/multiqc/main.nf index 2143f80b..62e47a2a 100644 --- a/modules/multiqc/main.nf +++ b/modules/multiqc/main.nf @@ -3,9 +3,8 @@ process multiqc { tag { sample } input: - tuple val(sample), path(variantscounts), path(stats) - // tuple val(sample), path('*') - // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) + // tuple val(sample), path(variantscounts), path(stats) + tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) output: From 90049f915c328131a8eeb99ce0f298fafc2e6c1e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 24 Jun 2024 02:26:54 +0000 Subject: [PATCH 049/132] update latest unittest run metrics result --- .../results/metrics/NA12878-chr14-AKT1.metrics.json | 11 +++++++++-- 1 file changed, 9 insertions(+), 2 deletions(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json index 9f2afb3a..8a33e1a2 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json @@ -3,14 +3,21 @@ "id": "NA12878-chr14-AKT1" }, "wgs_qc_metrics": { + "count_deletions": 848, + "count_insertions": 850, + "count_snvs": 8489, "cross_contamination_rate": "NA", "insert_size_std_deviation": 98.2, "mad_autosome_coverage": 0.0, - "mean_autosome_coverage": 0.00065, + "mean_autosome_coverage": 0.00064, "mean_insert_size": 447.3, - "pct_autosomes_15x": 0.00163, + "pct_autosomes_15x": 0.0016, "pct_reads_mapped": 99.6307, "pct_reads_properly_paired": 97.87835, + "ratio_heterozygous_homzygous_indel": 1.49, + "ratio_heterozygous_homzygous_snv": 1.05, + "ratio_insertion_deletion": 1.0, + "ratio_transitions_transversions": 2.13, "yield_bp_q30": 1996315 } } From aa010a3309933f85ae0e9233800e2ab84b92a754 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 2 Aug 2024 07:56:35 +0000 Subject: [PATCH 050/132] added ratio of het/hom insertion and deletion --- bin/compile_metrics.py | 2 +- bin/count_variants.py | 46 ++++++++ bin/count_variants.py.BAK | 239 ++++++++++++++++++++++++++++++++++++++ 3 files changed, 286 insertions(+), 1 deletion(-) create mode 100755 bin/count_variants.py.BAK diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index fdc7d5e4..e60a3bb1 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -58,7 +58,7 @@ def calculate_metrics(mqc): "count_snvs", "ratio_heterozygous_homzygous_snv", "ratio_heterozygous_homzygous_indel", "count_deletions", "count_insertions", "ratio_insertion_deletion", - "ratio_transitions_transversions" + "ratio_heterozygous_homzygous_insertion_deletion", "ratio_transitions_transversions" ] result = dict() logging.info("Calculating %d metrics..." % len(metrics_list)) diff --git a/bin/count_variants.py b/bin/count_variants.py index bcb0e263..c256005a 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -148,6 +148,52 @@ def count_variants(input_vcf, scratch_dir, regions): r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) + logging.info("Counting pass_del_het, pass_ins_het...") + cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS_HET + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HET\" }" + # if length(ALT) < length(REF), label as DEL_HET + cmd += " else if ( length($5) < length($4) ) { print \"DEL_HET\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Counting pass_del_hom, pass_ins_hom...") + cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS_HOM + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HOM\" }" + # if length(ALT) < length(REF), label as DEL_HOM + cmd += " else if ( length($5) < length($4) ) { print \"DEL_HOM\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + logging.info("Counting pass_del, pass_ins...") cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) # filter dragen annotation of variant records diff --git a/bin/count_variants.py.BAK b/bin/count_variants.py.BAK new file mode 100755 index 00000000..bcb0e263 --- /dev/null +++ b/bin/count_variants.py.BAK @@ -0,0 +1,239 @@ +#!/usr/bin/env python3 + +import logging +import argparse +import json +import pprint as pp +import subprocess +import numpy as np +import sys +import os +from pathlib import Path + + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--input_vcf", dest="input_vcf", required=True, + default=None, + help="Path to input VCF/gVCF.") + parser.add_argument("--regions", dest="regions", required=True, + default=None, + help="Path to input autosomes_non_gap_regions_bed.") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./variant_counts.json", + help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, + default="./", + help="Path to scratch dir. Default: ./") + parser.add_argument("--keep_scratch", dest="keep_scratch", required=False, + default=False, action="store_true", + help="Keep scratch dir. Default: False.") + parser.add_argument("--loglevel", dest="loglevel", required=False, + default="INFO", + help="Set logging level to INFO (default), WARNING or DEBUG.") + args = parser.parse_args() + + # create scratch dir if it doesn't exist + Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) + + return args + + +def set_logging(loglevel): + numeric_level = getattr(logging, loglevel.upper(), None) + if not isinstance(numeric_level, int): + raise ValueError('Invalid log level: %s' % loglevel) + logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', + level=numeric_level) + + +def setup(input_vcf, scratch_dir): + + # subset input vcf to include only variant sites to speed up execution from gvcf + logging.info("Subsetting variant sites from input vcf...") + variants_vcf = "%s/variants.vcf.gz" % scratch_dir + cmd = "bcftools view" + cmd += " -e 'GT=\"./.\" | GT=\".|.\" | GT=\".\" | GT=\"0/0\" | GT=\"0|0\" | GT=\"0\"'" + cmd += " -O z -o %s %s" % (variants_vcf, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + return variants_vcf + + +def count_variants(input_vcf, scratch_dir, regions): + + # initialise results dict + r = dict() + metrics_list = [ + "all_snps", + "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", + "all_indels", + "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", + "pass_del", "pass_ins", "pass_ins_del", + "pass_snp_ts_tv" + ] + for m in metrics_list: + r[m] = 0 + + # run bcftools stats - used to obtain ts/tv and sanity checks + logging.info("Running bcftools stats for all variants...") + cmd = "tabix -p vcf %s" % input_vcf + cmd1 = "bcftools stats -R %s %s > %s/all.bcftools_stats" % (regions, input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + logging.debug("CMD: %s" % cmd1) + p = subprocess.run(cmd, shell=True) + p = subprocess.run(cmd1, shell=True) + + logging.info("Running bcftools stats for PASS variants...") + cmd = "bcftools stats -f PASS -R %s %s > %s/pass.bcftools_stats" % (regions, input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + # calculate metrics + logging.info("Counting all_snps...") + cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_snps...") + cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_het_snps...") + cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_snps...") + cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_snps"] = int(p.stdout.read()) + + logging.info("Calculating pass_snp_het_hom...") + snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) + r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) + + logging.info("Counting all_indels...") + cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_indels...") + cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_het_indels...") + cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_indels...") + cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_indels"] = int(p.stdout.read()) + + logging.info("Calculating pass_indel_het_hom...") + indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) + r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) + + + logging.info("Counting pass_del, pass_ins...") + cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + # if length(ALT) < length(REF), label as DEL + cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Calculating pass_ins_del...") + ins_del = np.divide(r["pass_ins"], r["pass_del"]) + r["pass_ins_del"] = np.round(ins_del, 2) + + logging.info("Calculating pass_snp_ts_tv...") + cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + l = p.stdout.read().decode("utf-8").strip().split("\t") + r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) + + # sanity checks + logging.info("Running sanity checks for variant counts...") + + error_count = 0 + cmd = "cat %s/all.bcftools_stats | grep '^SN'" % scratch_dir + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + + for l in p.stdout.read().splitlines(): + l = l.decode("utf-8").strip().split("\t") + k = str(l[2]).lower() + v = int(l[3]) + if "snps" in k and v != r["all_snps"]: + logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) + error_count += 1 + elif "indels" in k and v != r["all_indels"]: + logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) + error_count += 1 + + if error_count > 0: + sys.exit("Sanity checks failed, aborting execution") + + return r + + +def save_output(d, outfile): + with open(outfile, "w") as f: + json.dump(d, f, sort_keys=True, indent=4) + f.write("\n") + + +def done(scratch_dir): + if not args.keep_scratch: + # clean up + logging.info("Cleaning up...") + os.remove("%s/variants.vcf.gz" % scratch_dir) + os.remove("%s/variants.vcf.gz.tbi" % scratch_dir) + os.remove("%s/all.bcftools_stats" % scratch_dir) + os.remove("%s/pass.bcftools_stats" % scratch_dir) + if len(os.listdir(scratch_dir)) == 0: + os.rmdir(scratch_dir) + else: + logging.warning("Couldn't remove scratch dir (%s): directory is not empty" % scratch_dir) + logging.info("DONE") + + +if __name__ == "__main__": + args = parse_args() + set_logging(args.loglevel) + + variants_vcf = setup(args.input_vcf, args.scratch_dir) + + vcf_metrics = count_variants(variants_vcf, args.scratch_dir, args.regions) + save_output(vcf_metrics, args.output_json) + + done(args.scratch_dir) From 46c98bb7e145d4b13d059ecb7a87a30664371724 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 2 Aug 2024 08:04:28 +0000 Subject: [PATCH 051/132] added ratio of het/hom insertion and deletion --- bin/count_variants.py | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/bin/count_variants.py b/bin/count_variants.py index c256005a..1bd909c7 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -217,6 +217,10 @@ def count_variants(input_vcf, scratch_dir, regions): v = int(l[0]) r[k] = v + logging.info("Calculating pass_ins_del_het_hom...") + ins_del_het_hom = np.divide(np.sum(r["pass_ins_het"], r["pass_del_het"]), np.sum(r["pass_ins_hom"], r["pass_del_hom"])) + r["pass_ins_del_het_hom"] = np.round(ins_del_het_hom, 2) + logging.info("Calculating pass_ins_del...") ins_del = np.divide(r["pass_ins"], r["pass_del"]) r["pass_ins_del"] = np.round(ins_del, 2) From a152f8179ff3b309a57109ef3cd2ef8ffb7c1680 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 2 Aug 2024 08:08:18 +0000 Subject: [PATCH 052/132] added ratio of het/hom insertion and deletion --- bin/count_variants.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/count_variants.py b/bin/count_variants.py index 1bd909c7..00e894b4 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -70,7 +70,7 @@ def count_variants(input_vcf, scratch_dir, regions): "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", "all_indels", "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", - "pass_del", "pass_ins", "pass_ins_del", + "pass_ins_del_het_hom", "pass_del", "pass_ins", "pass_ins_del", "pass_snp_ts_tv" ] for m in metrics_list: From e99c0d38b5bedef4662173690f13f4aa523db8b8 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 2 Aug 2024 08:08:29 +0000 Subject: [PATCH 053/132] added ratio of het/hom insertion and deletion --- bin/metrics/metrics.py.BAK | 313 +++++++++++++++++++++++++++++++++++++ 1 file changed, 313 insertions(+) create mode 100644 bin/metrics/metrics.py.BAK diff --git a/bin/metrics/metrics.py.BAK b/bin/metrics/metrics.py.BAK new file mode 100644 index 00000000..cafbe7b7 --- /dev/null +++ b/bin/metrics/metrics.py.BAK @@ -0,0 +1,313 @@ +#!/usr/bin/env python3 + +import numpy as np +import inspect + +DECIMALS = 5 + +def yield_bp_q30(mqc, biosample_id): + """ + Description: The number of bases in short paired-end sequencing high quality reads, primary alignments, achieving a base quality score of 30 or greater (Phred scale). Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using GATK Picard CollectQualityYieldMetrics, reporting the PF_Q30_BASES field. Only high quality bases from primary alignments are considered. No filter on duplicated reads, clipped bases or mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_picard_QualityYieldMetrics"][biosample_id] + v = d["PF_Q30_BASES"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pct_reads_mapped(mqc, biosample_id): + """ + Description: The percentage of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = np.divide(d["reads_mapped"], + d["sequences"]) + v = np.round(v*100, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def pct_reads_properly_paired(mqc, biosample_id): + """ + Description: The percentage of short paired-end sequencing high quality, properly paired reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of properly paired reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["reads_properly_paired_percent"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mean_autosome_coverage(mqc, biosample_id): + """ + Description: The mean sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing mean coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["MEAN_COVERAGE"] + v = np.round(np.float64(v), DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mad_autosome_coverage(mqc, biosample_id): + """ + Description: The median absolute deviation of sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing median absolute deviation coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["MAD_COVERAGE"] + v = np.round(np.float64(v), DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def pct_autosomes_15x(mqc, biosample_id): + """ + Description: The percentage of bases attaining at least 15X sequencing coverage in short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing coverage percentage of bases attaining at least 15X of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["PCT_15X"] + v = np.round(v*100, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mean_insert_size(mqc, biosample_id): + """ + Description: The mean insert size of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_average field. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["insert_size_average"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def insert_size_std_deviation(mqc, biosample_id): + """ + Description: The insert size standard deviation of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_standard_deviation field. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["insert_size_standard_deviation"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def cross_contamination_rate(mqc, biosample_id): + """ + Description: Estimation of inter-sample contamination rate of short paired-end sequencing high quality, non duplicated reads, primary alignments, mapped on GRCh38 assembly. No minimum mapping quality is imposed. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads and clipped bases. + + Implementation details: The estimation of inter-sample DNA contamination of short paired-end sequencing high quality, aligned sequence reads (BAM/CRAM) mapped on GRCh38 assembly with pre-calculated reference panel of 1000 Genome Project dataset from the VerifyBamID resource using VerifyBamID2 with NumPC “4” (# of Principal Components used in estimation), the key information “FREEMIX” in “.selfSM” in the results indicates the estimated contamination level. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_verifybamid"][biosample_id] + v = d["FREEMIX"] + except KeyError: + v = "NA" + + return k, v + +def count_snvs(mqc, biosample_id): + """ + The number of PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_het_snps(mqc, biosample_id): + """ + The number of PASS het SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_het_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_homalt_snps(mqc, biosample_id): + """ + The number of PASS hom alt SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_homalt_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def ratio_heterozygous_homzygous_snv(mqc, biosample_id): + """ + The het/hom ratio for PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snp_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def ratio_heterozygous_homzygous_indel(mqc, biosample_id): + """ + The het/hom ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_indel_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def count_deletions(mqc, biosample_id): + """ + The number of PASS deletions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_del"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def count_insertions(mqc, biosample_id): + """ + The number of PASS insertions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def ratio_insertion_deletion(mqc, biosample_id): + """ + The ins/del ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins_del"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + + +def ratio_transitions_transversions(mqc, biosample_id): + """ + The transition to transversion ratio of passing bi-allelic SNPs. + + Source: count_variants.py (bcftools stats - tstv) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snp_ts_tv"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v From 71ea6965fab2cd29ffddded1d221d86a5e7f2e16 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 2 Aug 2024 08:10:43 +0000 Subject: [PATCH 054/132] added ratio of het/hom insertion and deletion --- bin/metrics/metrics.py | 17 +++++++++++++++++ 1 file changed, 17 insertions(+) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index cafbe7b7..b09fd78a 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -243,6 +243,23 @@ def ratio_heterozygous_homzygous_indel(mqc, biosample_id): return k, v +def ratio_heterozygous_homzygous_insertion_deletion(mqc, biosample_id): + """ + The het/hom ratio for PASS insertions and deletions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins_del_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + def count_deletions(mqc, biosample_id): """ The number of PASS deletions. From 6f9fae4bfed94a1dff30ed772933ace1b8367485 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 6 Aug 2024 08:35:03 +0000 Subject: [PATCH 055/132] test reowrk code --- bin/compile_metrics.py | 2 +- bin/compile_metrics.py.UPDATE | 91 ++++++++++ bin/count_variants.py | 52 +----- bin/count_variants.py.UPDATE | 289 +++++++++++++++++++++++++++++ bin/metrics/metrics.py | 17 -- bin/metrics/metrics.py.UPDATE | 330 ++++++++++++++++++++++++++++++++++ 6 files changed, 712 insertions(+), 69 deletions(-) create mode 100755 bin/compile_metrics.py.UPDATE create mode 100755 bin/count_variants.py.UPDATE create mode 100644 bin/metrics/metrics.py.UPDATE diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index e60a3bb1..fdc7d5e4 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -58,7 +58,7 @@ def calculate_metrics(mqc): "count_snvs", "ratio_heterozygous_homzygous_snv", "ratio_heterozygous_homzygous_indel", "count_deletions", "count_insertions", "ratio_insertion_deletion", - "ratio_heterozygous_homzygous_insertion_deletion", "ratio_transitions_transversions" + "ratio_transitions_transversions" ] result = dict() logging.info("Calculating %d metrics..." % len(metrics_list)) diff --git a/bin/compile_metrics.py.UPDATE b/bin/compile_metrics.py.UPDATE new file mode 100755 index 00000000..e60a3bb1 --- /dev/null +++ b/bin/compile_metrics.py.UPDATE @@ -0,0 +1,91 @@ +#!/usr/bin/env python3 + +import logging +import argparse +import json +import metrics + + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--multiqc_json", dest="multiqc_json", required=True, + default=None, + help="Path to multiqc_data.json.") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./metrics.json", + help="Path to output json. Default: ./metrics.json") + parser.add_argument("--biosample_id", dest="biosample_id", required=True, + default=None, + help="Biosample ID. Default: None") + parser.add_argument("--loglevel", dest="loglevel", required=False, + default="INFO", + help="Set logging level to INFO (default), WARNING or DEBUG.") + args = parser.parse_args() + return args + + +def set_logging(loglevel): + numeric_level = getattr(logging, loglevel.upper(), None) + if not isinstance(numeric_level, int): + raise ValueError('Invalid log level: %s' % loglevel) + logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', + level=numeric_level) + + +def load_multi_qc(multiqc_json): + logging.info("Reading multiqc output: %s", multiqc_json) + with open(multiqc_json) as json_file: + data_metrics = json.load(json_file) + # keep raw data to simplify parsing downstream + mqc = data_metrics["report_saved_raw_data"] + return mqc + + +def calculate_metrics(mqc): + metrics_list = [ + # primary metrics + "yield_bp_q30", + # alignment + "pct_reads_mapped", "pct_reads_properly_paired", + # coverage + "mean_autosome_coverage", "mad_autosome_coverage", + "pct_autosomes_15x", + # insert size + "mean_insert_size", + "insert_size_std_deviation", + "cross_contamination_rate", + # variant calling + "count_snvs", "ratio_heterozygous_homzygous_snv", + "ratio_heterozygous_homzygous_indel", + "count_deletions", "count_insertions", "ratio_insertion_deletion", + "ratio_heterozygous_homzygous_insertion_deletion", "ratio_transitions_transversions" + ] + result = dict() + logging.info("Calculating %d metrics..." % len(metrics_list)) + for m in metrics_list: + k, v = eval("metrics." + m + "(mqc, args.biosample_id)") + result[k] = v + + return result + + +def save_output(data_metrics, outfile): + with open(outfile, "w") as f: + data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} + json.dump(data_metrics, f, sort_keys=True, indent=4) + f.write("\n") + + +def done(): + logging.info("DONE") + + +if __name__ == "__main__": + args = parse_args() + set_logging(args.loglevel) + + mqc = load_multi_qc(args.multiqc_json) + parsed_metrics = calculate_metrics(mqc) + save_output(parsed_metrics, args.output_json) + + done() diff --git a/bin/count_variants.py b/bin/count_variants.py index 00e894b4..bcb0e263 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -70,7 +70,7 @@ def count_variants(input_vcf, scratch_dir, regions): "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", "all_indels", "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", - "pass_ins_del_het_hom", "pass_del", "pass_ins", "pass_ins_del", + "pass_del", "pass_ins", "pass_ins_del", "pass_snp_ts_tv" ] for m in metrics_list: @@ -148,52 +148,6 @@ def count_variants(input_vcf, scratch_dir, regions): r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) - logging.info("Counting pass_del_het, pass_ins_het...") - cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS_HET - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HET\" }" - # if length(ALT) < length(REF), label as DEL_HET - cmd += " else if ( length($5) < length($4) ) { print \"DEL_HET\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - - logging.info("Counting pass_del_hom, pass_ins_hom...") - cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS_HOM - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HOM\" }" - # if length(ALT) < length(REF), label as DEL_HOM - cmd += " else if ( length($5) < length($4) ) { print \"DEL_HOM\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - logging.info("Counting pass_del, pass_ins...") cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) # filter dragen annotation of variant records @@ -217,10 +171,6 @@ def count_variants(input_vcf, scratch_dir, regions): v = int(l[0]) r[k] = v - logging.info("Calculating pass_ins_del_het_hom...") - ins_del_het_hom = np.divide(np.sum(r["pass_ins_het"], r["pass_del_het"]), np.sum(r["pass_ins_hom"], r["pass_del_hom"])) - r["pass_ins_del_het_hom"] = np.round(ins_del_het_hom, 2) - logging.info("Calculating pass_ins_del...") ins_del = np.divide(r["pass_ins"], r["pass_del"]) r["pass_ins_del"] = np.round(ins_del, 2) diff --git a/bin/count_variants.py.UPDATE b/bin/count_variants.py.UPDATE new file mode 100755 index 00000000..00e894b4 --- /dev/null +++ b/bin/count_variants.py.UPDATE @@ -0,0 +1,289 @@ +#!/usr/bin/env python3 + +import logging +import argparse +import json +import pprint as pp +import subprocess +import numpy as np +import sys +import os +from pathlib import Path + + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--input_vcf", dest="input_vcf", required=True, + default=None, + help="Path to input VCF/gVCF.") + parser.add_argument("--regions", dest="regions", required=True, + default=None, + help="Path to input autosomes_non_gap_regions_bed.") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./variant_counts.json", + help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, + default="./", + help="Path to scratch dir. Default: ./") + parser.add_argument("--keep_scratch", dest="keep_scratch", required=False, + default=False, action="store_true", + help="Keep scratch dir. Default: False.") + parser.add_argument("--loglevel", dest="loglevel", required=False, + default="INFO", + help="Set logging level to INFO (default), WARNING or DEBUG.") + args = parser.parse_args() + + # create scratch dir if it doesn't exist + Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) + + return args + + +def set_logging(loglevel): + numeric_level = getattr(logging, loglevel.upper(), None) + if not isinstance(numeric_level, int): + raise ValueError('Invalid log level: %s' % loglevel) + logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', + level=numeric_level) + + +def setup(input_vcf, scratch_dir): + + # subset input vcf to include only variant sites to speed up execution from gvcf + logging.info("Subsetting variant sites from input vcf...") + variants_vcf = "%s/variants.vcf.gz" % scratch_dir + cmd = "bcftools view" + cmd += " -e 'GT=\"./.\" | GT=\".|.\" | GT=\".\" | GT=\"0/0\" | GT=\"0|0\" | GT=\"0\"'" + cmd += " -O z -o %s %s" % (variants_vcf, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + return variants_vcf + + +def count_variants(input_vcf, scratch_dir, regions): + + # initialise results dict + r = dict() + metrics_list = [ + "all_snps", + "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", + "all_indels", + "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", + "pass_ins_del_het_hom", "pass_del", "pass_ins", "pass_ins_del", + "pass_snp_ts_tv" + ] + for m in metrics_list: + r[m] = 0 + + # run bcftools stats - used to obtain ts/tv and sanity checks + logging.info("Running bcftools stats for all variants...") + cmd = "tabix -p vcf %s" % input_vcf + cmd1 = "bcftools stats -R %s %s > %s/all.bcftools_stats" % (regions, input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + logging.debug("CMD: %s" % cmd1) + p = subprocess.run(cmd, shell=True) + p = subprocess.run(cmd1, shell=True) + + logging.info("Running bcftools stats for PASS variants...") + cmd = "bcftools stats -f PASS -R %s %s > %s/pass.bcftools_stats" % (regions, input_vcf, scratch_dir) + logging.debug("CMD: %s" % cmd) + p = subprocess.run(cmd, shell=True) + + # calculate metrics + logging.info("Counting all_snps...") + cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_snps...") + cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_het_snps...") + cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_snps"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_snps...") + cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_snps"] = int(p.stdout.read()) + + logging.info("Calculating pass_snp_het_hom...") + snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) + r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) + + logging.info("Counting all_indels...") + cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["all_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_indels...") + cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_het_indels...") + cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_het_indels"] = int(p.stdout.read()) + + logging.info("Counting pass_homalt_indels...") + cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + r["pass_homalt_indels"] = int(p.stdout.read()) + + logging.info("Calculating pass_indel_het_hom...") + indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) + r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) + + + logging.info("Counting pass_del_het, pass_ins_het...") + cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS_HET + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HET\" }" + # if length(ALT) < length(REF), label as DEL_HET + cmd += " else if ( length($5) < length($4) ) { print \"DEL_HET\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Counting pass_del_hom, pass_ins_hom...") + cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS_HOM + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HOM\" }" + # if length(ALT) < length(REF), label as DEL_HOM + cmd += " else if ( length($5) < length($4) ) { print \"DEL_HOM\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Counting pass_del, pass_ins...") + cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) + # filter dragen annotation of variant records + cmd += " | sed 's/,//g'" + # exclude multi-allelic sites + cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" + # if length(ALT) > length(REF), label as INS + cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + # if length(ALT) < length(REF), label as DEL + cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + # label everything else as UNK and report its location + cmd += " else { print \"UNK\" } }'" + # count by label + cmd += " | sort | uniq -c" + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + x = p.stdout.read() + for l in x.splitlines(): + l = l.decode("utf-8").strip().split(" ") + k = "pass_%s" % l[1].lower() + v = int(l[0]) + r[k] = v + + logging.info("Calculating pass_ins_del_het_hom...") + ins_del_het_hom = np.divide(np.sum(r["pass_ins_het"], r["pass_del_het"]), np.sum(r["pass_ins_hom"], r["pass_del_hom"])) + r["pass_ins_del_het_hom"] = np.round(ins_del_het_hom, 2) + + logging.info("Calculating pass_ins_del...") + ins_del = np.divide(r["pass_ins"], r["pass_del"]) + r["pass_ins_del"] = np.round(ins_del, 2) + + logging.info("Calculating pass_snp_ts_tv...") + cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir + logging.debug("CMD: %s" % cmd) + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + l = p.stdout.read().decode("utf-8").strip().split("\t") + r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) + + # sanity checks + logging.info("Running sanity checks for variant counts...") + + error_count = 0 + cmd = "cat %s/all.bcftools_stats | grep '^SN'" % scratch_dir + p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) + + for l in p.stdout.read().splitlines(): + l = l.decode("utf-8").strip().split("\t") + k = str(l[2]).lower() + v = int(l[3]) + if "snps" in k and v != r["all_snps"]: + logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) + error_count += 1 + elif "indels" in k and v != r["all_indels"]: + logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) + error_count += 1 + + if error_count > 0: + sys.exit("Sanity checks failed, aborting execution") + + return r + + +def save_output(d, outfile): + with open(outfile, "w") as f: + json.dump(d, f, sort_keys=True, indent=4) + f.write("\n") + + +def done(scratch_dir): + if not args.keep_scratch: + # clean up + logging.info("Cleaning up...") + os.remove("%s/variants.vcf.gz" % scratch_dir) + os.remove("%s/variants.vcf.gz.tbi" % scratch_dir) + os.remove("%s/all.bcftools_stats" % scratch_dir) + os.remove("%s/pass.bcftools_stats" % scratch_dir) + if len(os.listdir(scratch_dir)) == 0: + os.rmdir(scratch_dir) + else: + logging.warning("Couldn't remove scratch dir (%s): directory is not empty" % scratch_dir) + logging.info("DONE") + + +if __name__ == "__main__": + args = parse_args() + set_logging(args.loglevel) + + variants_vcf = setup(args.input_vcf, args.scratch_dir) + + vcf_metrics = count_variants(variants_vcf, args.scratch_dir, args.regions) + save_output(vcf_metrics, args.output_json) + + done(args.scratch_dir) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index b09fd78a..cafbe7b7 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -243,23 +243,6 @@ def ratio_heterozygous_homzygous_indel(mqc, biosample_id): return k, v -def ratio_heterozygous_homzygous_insertion_deletion(mqc, biosample_id): - """ - The het/hom ratio for PASS insertions and deletions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins_del_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - def count_deletions(mqc, biosample_id): """ The number of PASS deletions. diff --git a/bin/metrics/metrics.py.UPDATE b/bin/metrics/metrics.py.UPDATE new file mode 100644 index 00000000..b09fd78a --- /dev/null +++ b/bin/metrics/metrics.py.UPDATE @@ -0,0 +1,330 @@ +#!/usr/bin/env python3 + +import numpy as np +import inspect + +DECIMALS = 5 + +def yield_bp_q30(mqc, biosample_id): + """ + Description: The number of bases in short paired-end sequencing high quality reads, primary alignments, achieving a base quality score of 30 or greater (Phred scale). Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using GATK Picard CollectQualityYieldMetrics, reporting the PF_Q30_BASES field. Only high quality bases from primary alignments are considered. No filter on duplicated reads, clipped bases or mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_picard_QualityYieldMetrics"][biosample_id] + v = d["PF_Q30_BASES"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pct_reads_mapped(mqc, biosample_id): + """ + Description: The percentage of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = np.divide(d["reads_mapped"], + d["sequences"]) + v = np.round(v*100, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def pct_reads_properly_paired(mqc, biosample_id): + """ + Description: The percentage of short paired-end sequencing high quality, properly paired reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of properly paired reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["reads_properly_paired_percent"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mean_autosome_coverage(mqc, biosample_id): + """ + Description: The mean sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing mean coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["MEAN_COVERAGE"] + v = np.round(np.float64(v), DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mad_autosome_coverage(mqc, biosample_id): + """ + Description: The median absolute deviation of sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing median absolute deviation coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["MAD_COVERAGE"] + v = np.round(np.float64(v), DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def pct_autosomes_15x(mqc, biosample_id): + """ + Description: The percentage of bases attaining at least 15X sequencing coverage in short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. + + Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing coverage percentage of bases attaining at least 15X of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_picard_wgsmetrics"][biosample_id] + v = d["PCT_15X"] + v = np.round(v*100, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def mean_insert_size(mqc, biosample_id): + """ + Description: The mean insert size of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_average field. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["insert_size_average"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def insert_size_std_deviation(mqc, biosample_id): + """ + Description: The insert size standard deviation of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. + + Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_standard_deviation field. Duplicated reads are included. No mapping qualiy is applied. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_samtools_stats"][biosample_id] + v = d["insert_size_standard_deviation"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def cross_contamination_rate(mqc, biosample_id): + """ + Description: Estimation of inter-sample contamination rate of short paired-end sequencing high quality, non duplicated reads, primary alignments, mapped on GRCh38 assembly. No minimum mapping quality is imposed. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads and clipped bases. + + Implementation details: The estimation of inter-sample DNA contamination of short paired-end sequencing high quality, aligned sequence reads (BAM/CRAM) mapped on GRCh38 assembly with pre-calculated reference panel of 1000 Genome Project dataset from the VerifyBamID resource using VerifyBamID2 with NumPC “4” (# of Principal Components used in estimation), the key information “FREEMIX” in “.selfSM” in the results indicates the estimated contamination level. + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_verifybamid"][biosample_id] + v = d["FREEMIX"] + except KeyError: + v = "NA" + + return k, v + +def count_snvs(mqc, biosample_id): + """ + The number of PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_het_snps(mqc, biosample_id): + """ + The number of PASS het SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_het_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def pass_homalt_snps(mqc, biosample_id): + """ + The number of PASS hom alt SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_homalt_snps"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def ratio_heterozygous_homzygous_snv(mqc, biosample_id): + """ + The het/hom ratio for PASS SNPs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snp_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def ratio_heterozygous_homzygous_indel(mqc, biosample_id): + """ + The het/hom ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_indel_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def ratio_heterozygous_homzygous_insertion_deletion(mqc, biosample_id): + """ + The het/hom ratio for PASS insertions and deletions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins_del_het_hom"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + +def count_deletions(mqc, biosample_id): + """ + The number of PASS deletions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_del"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def count_insertions(mqc, biosample_id): + """ + The number of PASS insertions. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins"] + v = int(v) + except KeyError: + v = "NA" + + return k, v + +def ratio_insertion_deletion(mqc, biosample_id): + """ + The ins/del ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins_del"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + + +def ratio_transitions_transversions(mqc, biosample_id): + """ + The transition to transversion ratio of passing bi-allelic SNPs. + + Source: count_variants.py (bcftools stats - tstv) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_snp_ts_tv"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v From 1dc1f83bc484b5cb04e0d88eb00b05ad70ded24e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 7 Aug 2024 03:19:21 +0000 Subject: [PATCH 056/132] code update to generate metrics key value pair --- modules/CollectMultipleMetrics/main.nf | 3 +++ modules/CollectWgsMetrics/main.nf | 5 +++++ modules/samtools/main.nf | 5 +++++ modules/verifybamid2/main.nf | 2 ++ 4 files changed, 15 insertions(+) diff --git a/modules/CollectMultipleMetrics/main.nf b/modules/CollectMultipleMetrics/main.nf index 7e14f482..768d914c 100644 --- a/modules/CollectMultipleMetrics/main.nf +++ b/modules/CollectMultipleMetrics/main.nf @@ -10,6 +10,7 @@ process picard_collect_multiple_metrics { output: tuple val(sample), path("${sample}.quality_yield_metrics.txt"), emit: quality tuple val(sample), path("${sample}.insert_size_metrics.txt"), emit: insert_size + tuple val(sample), path("${sample}.quality_yield_metrics.metrics"), emit: metrics script: def reference = ref_fasta ? /R="${ref_fasta}"/ : '' @@ -28,5 +29,7 @@ process picard_collect_multiple_metrics { PROGRAM=CollectInsertSizeMetrics \ METRIC_ACCUMULATION_LEVEL=null \ METRIC_ACCUMULATION_LEVEL=ALL_READS + + cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "{yield_bp_q30: ", \$1,"}"}' > "${sample}.quality_yield_metrics.metrics" """ } diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index 4113988f..a0af01e6 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -10,6 +10,7 @@ process picard_collect_wgs_metrics { output: tuple val(sample), path("${sample}_wgs_metrics.txt"), emit: wgs_coverage + tuple val(sample), path("${sample}_wgs_metrics.metrics"), emit: metrics script: """ @@ -21,5 +22,9 @@ process picard_collect_wgs_metrics { R=${ref_fasta} \ INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT + + cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "{mean_autosome_coverage: ", \$1,"}"}' > "${sample}_wgs_metrics.metrics" + cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "{pct_autosomes_15x: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index ca4412bf..652c67aa 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -8,6 +8,7 @@ process samtools_stats { output: tuple val(sample), path("${sample}.stats"), emit: stats + tuple val(sample), path("${sample}.samtools.metrics") script: def reference = ref_fasta ? /--reference "${ref_fasta}"/ : '' @@ -21,5 +22,9 @@ process samtools_stats { "${bam}" \\ --threads ${task.cpus} \\ > "${sample}.stats" + + grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "{mean_insert_size: ", \$1,"}"}' >"${sample}.samtools.metrics" + grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "{insert_size_std_deviation: ", \$1,"}"}' >>"${sample}.samtools.metrics" + grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" """ } diff --git a/modules/verifybamid2/main.nf b/modules/verifybamid2/main.nf index 4662f2d5..58e1ea4f 100644 --- a/modules/verifybamid2/main.nf +++ b/modules/verifybamid2/main.nf @@ -12,6 +12,7 @@ process verifybamid2 { output: tuple val(sample), path("${sample}.selfSM"), emit: freemix tuple val(sample), path("${sample}.Ancestry"), emit: ancestry + tuple val(sample), path("${sample}.selfSM.metrics"), emit: metrics script: def reference = ref_fasta ? /--Reference "${ref_fasta}"/ : '' @@ -22,6 +23,7 @@ process verifybamid2 { verifybamid2 --NumPC 4 --NumThread ${task.cpus*2} --SVDPrefix ${params.vbi2_svdprefix} ${reference} --BamFile ${bam} --Output ${sample} + cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "{cross_contamination_rate: ", \$1,"}"}' > "${sample}.selfSM.metrics" """ } From 53df2a038af669c7eb779bd478b7047b2a6bffb8 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 12 Aug 2024 01:43:47 +0000 Subject: [PATCH 057/132] update aln, vcf metric key value pait code --- modules/count_aln_vcf/main.nf | 14 ++++++++++++++ modules/count_variants/main.nf | 4 ++-- modules/samtools/main.nf | 2 +- nextflow.config | 26 ++++++++++++++++++++++++-- 4 files changed, 41 insertions(+), 5 deletions(-) create mode 100644 modules/count_aln_vcf/main.nf diff --git a/modules/count_aln_vcf/main.nf b/modules/count_aln_vcf/main.nf new file mode 100644 index 00000000..34d7094d --- /dev/null +++ b/modules/count_aln_vcf/main.nf @@ -0,0 +1,14 @@ +process count_aln_vcf { + + tag { sample } + + input: + tuple val(sample), path(count_aln), path(count_variants) + + output: + tuple val(sample), path("${sample}.aln.variant.metrics.json"), emit: metrics + + """ + cat ${count_aln} ${count_variants} > "${sample}.aln.variant.metrics.json" + """ +} diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index bb0dc193..0b5511ba 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -7,14 +7,14 @@ process count_variants { path(autosomes_non_gap_regions) output: - tuple val(sample), path("${sample}.variant_counts.json") + tuple val(sample), path("${sample}.variant.metrics.json") script: """ count_variants.py \ --input_vcf ${vcf} \ --regions ${autosomes_non_gap_regions} \ - --output_json ${sample}.variant_counts.json \ + --output_json ${sample}.variant.metrics.json \ --loglevel DEBUG """ diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 652c67aa..833fc730 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -8,7 +8,7 @@ process samtools_stats { output: tuple val(sample), path("${sample}.stats"), emit: stats - tuple val(sample), path("${sample}.samtools.metrics") + tuple val(sample), path("${sample}.samtools.metrics"), emit: metrics script: def reference = ref_fasta ? /--reference "${ref_fasta}"/ : '' diff --git a/nextflow.config b/nextflow.config index a149ae9a..426ba756 100644 --- a/nextflow.config +++ b/nextflow.config @@ -108,10 +108,32 @@ process { memory = '1 GB' publishDir = [ - path: "${params.publishdir}/count_variants", + path: "${params.publishdir}/metrics", mode: params.publish_mode, ] - } + } + withName: 'count_aln' { + + cpus = 1 + disk = '50 GB' + memory = { 2.GB * task.attempt } + + publishDir = [ + path: "${params.publishdir}/metrics", + mode: params.publish_mode, + ] + } + withName: 'count_aln_vcf' { + + cpus = 1 + disk = '50 GB' + memory = { 2.GB * task.attempt } + + publishDir = [ + path: "${params.publishdir}/metrics", + mode: params.publish_mode, + ] + } withName: 'multiqc' { cpus = 1 From 4b80c8c0ad745a058f8babf9206529996ccc4b5c Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 26 Aug 2024 03:18:32 +0000 Subject: [PATCH 058/132] tested for count aln metrics only --- main.nf | 68 ++++++++++++++++++++++++++++++++------- modules/count_aln/main.nf | 14 ++++++++ modules/samtools/main.nf | 10 +++++- 3 files changed, 80 insertions(+), 12 deletions(-) create mode 100644 modules/count_aln/main.nf diff --git a/main.nf b/main.nf index 81dd44e0..700b6efe 100644 --- a/main.nf +++ b/main.nf @@ -95,6 +95,8 @@ include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from ' include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' include { bcftools_stats } from './modules/bcftools' include { count_variants } from './modules/count_variants' +include { count_aln } from './modules/count_aln' +include { count_aln_vcf } from './modules/count_aln_vcf' include { multiqc } from './modules/multiqc' /* @@ -140,6 +142,19 @@ workflow { .set { aln_inputs } + Channel + samples.branch { rec -> + def aln_file = rec.aln ? file( rec.aln ) : null + def vcf_file = rec.vcf ? file( rec.vcf ) : null + + count: rec.biosample_id && aln_file && vcf_file + + return tuple( rec.biosample_id ) + } + .view() + .set { inputs_cond } + + samtools_stats_bam( aln_inputs.bam, [] ) samtools_stats_cram( aln_inputs.cram, ref_fasta ) @@ -186,24 +201,43 @@ workflow { Channel .empty() sample_ids - .join( samtools_stats_bam.out.stats ) - .join( picard_collect_multiple_metrics_bam.out.insert_size ) - .join( picard_collect_multiple_metrics_bam.out.quality ) - .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) - .join( verifybamid2_bam.out.freemix, remainder: true ) + .join( samtools_stats_bam.out.metrics ) + .join( picard_collect_multiple_metrics_bam.out.metrics ) + .join( picard_collect_wgs_metrics_bam.out.metrics ) + .join( verifybamid2_bam.out.metrics, remainder: true ) .set { ch_bam } // channel for samplelist input file type cram processed outputs Channel .empty() sample_ids - .join( samtools_stats_cram.out.stats ) - .join( picard_collect_multiple_metrics_cram.out.insert_size ) - .join( picard_collect_multiple_metrics_cram.out.quality ) - .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) - .join( verifybamid2_cram.out.freemix, remainder: true ) + .join( samtools_stats_cram.out.metrics ) + .join( picard_collect_multiple_metrics_cram.out.metrics ) + .join( picard_collect_wgs_metrics_cram.out.metrics ) + .join( verifybamid2_cram.out.metrics, remainder: true ) .set { ch_cram } + +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + ch_bam.mix(ch_cram) + .map { sample, stats, quality, wgs_coverage, freemix -> [ sample, stats, quality, wgs_coverage, freemix ?: [] ] } + .set { aln_count_in } + + count_aln ( aln_count_in ) + + +// channel for samplelist input file type bam processed outputs + Channel + .empty() + sample_ids + .join( inputs_cond ) + .join( count_aln.out.metrics ) + .join( count_variants.out ) + .view() + .set { ch_count } + + count_aln_vcf ( ch_count ) + /* // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty ch_bam.mix(ch_cram) @@ -213,6 +247,8 @@ workflow { //.view() .set { multiqc_in } */ + +/* // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty ch_bam.mix(ch_cram) // .ifEmpty([]) //.combine(vcf_qc,by:0) @@ -223,6 +259,8 @@ workflow { .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } .view() .set { multiqc_in } +*/ + /* @@ -250,7 +288,15 @@ workflow { .set { multiqc_in } */ - multiqc( multiqc_in ) +/* + metrics_combine = Channel.empty() + if (params.vcf && params.aln) { + metrics_combine = ( count_variants.out ) + .view() + } +*/ + +// multiqc( multiqc_in ) } diff --git a/modules/count_aln/main.nf b/modules/count_aln/main.nf new file mode 100644 index 00000000..113b876c --- /dev/null +++ b/modules/count_aln/main.nf @@ -0,0 +1,14 @@ +process count_aln { + + tag { sample } + + input: + tuple val(sample), path(stats), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix) + + output: + tuple val(sample), path("${sample}.aln.metrics.json"), emit: metrics + + """ + cat ${stats} ${picard_quality} ${picard_wgs_coverage} ${verifybamid_freemix} > "${sample}.aln.metrics.json" + """ +} diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 833fc730..e4b79e50 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -25,6 +25,14 @@ process samtools_stats { grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "{mean_insert_size: ", \$1,"}"}' >"${sample}.samtools.metrics" grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "{insert_size_std_deviation: ", \$1,"}"}' >>"${sample}.samtools.metrics" - grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" + grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t", \$1}' >>"${sample}.samtools.metrics" + #grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" + seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) + mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) + #echo \$mapped_reads \$seq >>"${sample}.samtools.metrics" + awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t %.2f", 100 * num / denom) }' >>"${sample}.samtools.metrics" + #awk "BEGIN { printf 100 * \$mapped_reads / \$seq }" >>"${sample}.samtools.metrics" + #div=\$((mapped_reads*100 / seq )) + #echo \$div >>"${sample}.samtools.metrics" """ } From ed1c46b5210c826e09ed0f0966e95cc7ed0fccdc Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 17:26:22 +0000 Subject: [PATCH 059/132] update standalone aln, variants and combined metrics --- bin/count_variants.py | 55 +++++++++++++++++--------- modules/CollectMultipleMetrics/main.nf | 2 +- modules/CollectWgsMetrics/main.nf | 7 ++-- modules/count_aln/main.nf | 7 +++- modules/count_variants/main.nf | 1 + modules/samtools/main.nf | 13 +++--- modules/verifybamid2/main.nf | 2 +- 7 files changed, 54 insertions(+), 33 deletions(-) diff --git a/bin/count_variants.py b/bin/count_variants.py index bcb0e263..47d8a35c 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -22,6 +22,9 @@ def parse_args(): parser.add_argument("--output_json", dest="output_json", required=False, default="./variant_counts.json", help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--biosample_id", dest="biosample_id", required=True, + default=None, + help="Sample ID.") parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, default="./", help="Path to scratch dir. Default: ./") @@ -66,10 +69,8 @@ def count_variants(input_vcf, scratch_dir, regions): # initialise results dict r = dict() metrics_list = [ - "all_snps", - "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", - "all_indels", - "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", + "pass_snps", "pass_snp_het_hom", + "pass_indel_het_hom", "pass_del", "pass_ins", "pass_ins_del", "pass_snp_ts_tv" ] @@ -95,7 +96,8 @@ def count_variants(input_vcf, scratch_dir, regions): cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_snps"] = int(p.stdout.read()) + # r["all_snps"] = int(p.stdout.read()) + all_snps = int(p.stdout.read()) logging.info("Counting pass_snps...") cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) @@ -107,44 +109,53 @@ def count_variants(input_vcf, scratch_dir, regions): cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_snps"] = int(p.stdout.read()) + # r["pass_het_snps"] = int(p.stdout.read()) + pass_het_snps = int(p.stdout.read()) logging.info("Counting pass_homalt_snps...") cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_snps"] = int(p.stdout.read()) + # r["pass_homalt_snps"] = int(p.stdout.read()) + pass_homalt_snps = int(p.stdout.read()) + logging.info("Calculating pass_snp_het_hom...") - snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) + # snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) + snp_het_hom = np.divide(pass_het_snps, pass_homalt_snps) r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) logging.info("Counting all_indels...") cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_indels"] = int(p.stdout.read()) + # r["all_indels"] = int(p.stdout.read()) + all_indels = int(p.stdout.read()) logging.info("Counting pass_indels...") cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_indels"] = int(p.stdout.read()) + # r["pass_indels"] = int(p.stdout.read()) + pass_indels = int(p.stdout.read()) logging.info("Counting pass_het_indels...") cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_indels"] = int(p.stdout.read()) + # r["pass_het_indels"] = int(p.stdout.read()) + pass_het_indels = int(p.stdout.read()) logging.info("Counting pass_homalt_indels...") cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_indels"] = int(p.stdout.read()) + # r["pass_homalt_indels"] = int(p.stdout.read()) + pass_homalt_indels = int(p.stdout.read()) logging.info("Calculating pass_indel_het_hom...") - indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) + # indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) + indel_het_hom = np.divide(pass_het_indels, pass_homalt_indels) r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) @@ -193,11 +204,15 @@ def count_variants(input_vcf, scratch_dir, regions): l = l.decode("utf-8").strip().split("\t") k = str(l[2]).lower() v = int(l[3]) - if "snps" in k and v != r["all_snps"]: - logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) + if "snps" in k and v != all_snps: + #if "snps" in k and v != r["all_snps"]: + # logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) + logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, all_snps)) error_count += 1 - elif "indels" in k and v != r["all_indels"]: - logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) + # elif "indels" in k and v != r["all_indels"]: + elif "indels" in k and v != all_indels: + # logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) + logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, all_indels)) error_count += 1 if error_count > 0: @@ -206,9 +221,11 @@ def count_variants(input_vcf, scratch_dir, regions): return r -def save_output(d, outfile): +def save_output(data_metrics, outfile): with open(outfile, "w") as f: - json.dump(d, f, sort_keys=True, indent=4) + # json.dump(data_metrics, f, sort_keys=True, indent=4) + data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} + json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") diff --git a/modules/CollectMultipleMetrics/main.nf b/modules/CollectMultipleMetrics/main.nf index 768d914c..84c2548c 100644 --- a/modules/CollectMultipleMetrics/main.nf +++ b/modules/CollectMultipleMetrics/main.nf @@ -30,6 +30,6 @@ process picard_collect_multiple_metrics { METRIC_ACCUMULATION_LEVEL=null \ METRIC_ACCUMULATION_LEVEL=ALL_READS - cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "{yield_bp_q30: ", \$1,"}"}' > "${sample}.quality_yield_metrics.metrics" + cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30\t", \$1}' > "${sample}.quality_yield_metrics.metrics" """ } diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index a0af01e6..02fdec83 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -23,8 +23,9 @@ process picard_collect_wgs_metrics { INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT - cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "{mean_autosome_coverage: ", \$1,"}"}' > "${sample}_wgs_metrics.metrics" - cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "{pct_autosomes_15x: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" - cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" + cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage", \$1}' > "${sample}_wgs_metrics.metrics" + cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x", \$1}' >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage", \$1}' >> "${sample}_wgs_metrics.metrics" + # cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/count_aln/main.nf b/modules/count_aln/main.nf index 113b876c..158ef942 100644 --- a/modules/count_aln/main.nf +++ b/modules/count_aln/main.nf @@ -9,6 +9,11 @@ process count_aln { tuple val(sample), path("${sample}.aln.metrics.json"), emit: metrics """ - cat ${stats} ${picard_quality} ${picard_wgs_coverage} ${verifybamid_freemix} > "${sample}.aln.metrics.json" + cat ${stats} ${picard_quality} ${picard_wgs_coverage} ${verifybamid_freemix} > "${sample}.aln.list.txt" + + count_aln.py \ + --input_metrics "${sample}.aln.list.txt" \ + --sample_id ${sample} \ + --output_json ${sample}.aln_metrics.json """ } diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index 0b5511ba..7cf1ee4e 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -15,6 +15,7 @@ process count_variants { --input_vcf ${vcf} \ --regions ${autosomes_non_gap_regions} \ --output_json ${sample}.variant.metrics.json \ + --biosample_id ${sample} \ --loglevel DEBUG """ diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index e4b79e50..63d79e5b 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -23,16 +23,13 @@ process samtools_stats { --threads ${task.cpus} \\ > "${sample}.stats" - grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "{mean_insert_size: ", \$1,"}"}' >"${sample}.samtools.metrics" - grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "{insert_size_std_deviation: ", \$1,"}"}' >>"${sample}.samtools.metrics" + grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size", \$1}' >"${sample}.samtools.metrics" + grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t", \$1}' >>"${sample}.samtools.metrics" grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t", \$1}' >>"${sample}.samtools.metrics" - #grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" + # grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) - #echo \$mapped_reads \$seq >>"${sample}.samtools.metrics" - awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t %.2f", 100 * num / denom) }' >>"${sample}.samtools.metrics" - #awk "BEGIN { printf 100 * \$mapped_reads / \$seq }" >>"${sample}.samtools.metrics" - #div=\$((mapped_reads*100 / seq )) - #echo \$div >>"${sample}.samtools.metrics" + div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t %.2f", 100 * num / denom) }') + echo \$div >>"${sample}.samtools.metrics" """ } diff --git a/modules/verifybamid2/main.nf b/modules/verifybamid2/main.nf index 58e1ea4f..7546624b 100644 --- a/modules/verifybamid2/main.nf +++ b/modules/verifybamid2/main.nf @@ -23,7 +23,7 @@ process verifybamid2 { verifybamid2 --NumPC 4 --NumThread ${task.cpus*2} --SVDPrefix ${params.vbi2_svdprefix} ${reference} --BamFile ${bam} --Output ${sample} - cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "{cross_contamination_rate: ", \$1,"}"}' > "${sample}.selfSM.metrics" + cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate\t", \$1}' > "${sample}.selfSM.metrics" """ } From 6b674d1142ae043811f6e141a3f1eee1e3daa298 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 17:35:47 +0000 Subject: [PATCH 060/132] Dockerfile update git branch --- containers/Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index c4b27c92..c7136662 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -50,7 +50,7 @@ RUN conda clean -a RUN git clone https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin/multiqc_plugins -RUN git checkout feature/vcf-qc-implementation +RUN git checkout feature/rework-aln-variant-metrics-json RUN python3 setup.py install # RUN python -m pip install . # RUN pip3 install numpy scipy argparse matplotlib From 7949223998643ec23bfca6fdc000a1d7cb80585e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 17:42:18 +0000 Subject: [PATCH 061/132] nextflow.config uncomment the cleanup --- nextflow.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow.config b/nextflow.config index 426ba756..f3d63368 100644 --- a/nextflow.config +++ b/nextflow.config @@ -6,12 +6,12 @@ manifest { description = 'Nextflow NPM-sample-qc analysis pipeline, part of the GHIF community.' mainScript = 'main.nf' nextflowVersion = '!>=23.10.1' - version = '0.12.0' + version = '0.13.0' } -docker_version = '0.12.0' +docker_version = '0.13.0' -// cleanup = true +cleanup = true params { From 71b5bd87dbf9552550e45062341329e726a4be45 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 17:49:35 +0000 Subject: [PATCH 062/132] update standalone aln, variants and combined metrics --- bin/count_aln.py | 70 ++++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 70 insertions(+) create mode 100755 bin/count_aln.py diff --git a/bin/count_aln.py b/bin/count_aln.py new file mode 100755 index 00000000..5ac189e5 --- /dev/null +++ b/bin/count_aln.py @@ -0,0 +1,70 @@ +#!/usr/bin/env python3 + +import argparse +import json +import pprint as pp +import subprocess +import numpy as np +import sys +import os +from pathlib import Path + + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--sample_id", dest="sample_id", required=True, + default=None, + help="Sample ID") + parser.add_argument("--input_metrics", dest="input_metrics", required=True, + default=None, + help="Path to input aln metrics list") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./variant_counts.json", + help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, + default="./", + help="Path to scratch dir. Default: ./") + args = parser.parse_args() + + # create scratch dir if it doesn't exist + Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) + + return args + + +#def raw_data(input_metrics): +# #d = dict() +# d = {} +# with open(input_metrics) as f: +# rows = ( line.split('\t') for line in f ) +# d = { row[0]:row[1] for row in rows } +# return d + +def raw_data(input_metrics): + d = {} + with open(input_metrics) as f: + for line in f: + if not line.strip(): + continue + row = line.split('\t') + key = row[0] + value_str = row[1] + try: + value = float(value_str.strip()) + except ValueError: + value = value_str.strip() + d[key] = value + return d + + +def save_output(data_metrics, outfile): + with open(outfile, "w") as f: + data_metrics = {"sample" : {"id" : args.sample_id}, "wgs_metrics" : data_metrics} + json.dump(data_metrics, f, sort_keys=True, indent=4) + f.write("\n") + +if __name__ == "__main__": + args = parse_args() + + data_metrics = raw_data(args.input_metrics) + save_output(data_metrics, args.output_json) From 712cca1dbca10e14e22b1ae1ecc01f16e292ff24 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 18:04:06 +0000 Subject: [PATCH 063/132] Dockerfile update git branch --- containers/Dockerfile | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index c7136662..5fb9f175 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -46,11 +46,11 @@ RUN conda clean -a # install MultiQC and MultiQC_NPM plugin # RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git -#RUN git clone -b feature/vcf-qc-implementation --single-branch https://github.com/c-BIG/NPM-sample-qc.git -RUN git clone https://github.com/c-BIG/NPM-sample-qc.git +RUN git clone -b feature/vcf-qc-implementation https://github.com/c-BIG/NPM-sample-qc.git +# RUN git clone https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin/multiqc_plugins -RUN git checkout feature/rework-aln-variant-metrics-json +# RUN git checkout feature/rework-aln-variant-metrics-json RUN python3 setup.py install # RUN python -m pip install . # RUN pip3 install numpy scipy argparse matplotlib From 81611864616339b1b338433a1677261341c62ae9 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 18:58:30 +0000 Subject: [PATCH 064/132] update git branch in Dockerfile --- containers/Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index 5fb9f175..aeab904f 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -46,7 +46,7 @@ RUN conda clean -a # install MultiQC and MultiQC_NPM plugin # RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git -RUN git clone -b feature/vcf-qc-implementation https://github.com/c-BIG/NPM-sample-qc.git +RUN git clone -b feature/rework-aln-variant-metrics-json https://github.com/c-BIG/NPM-sample-qc.git # RUN git clone https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin/multiqc_plugins From 2d8e3380aa23690f6672abe73cb43fcb0f61cee1 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 29 Aug 2024 19:11:27 +0000 Subject: [PATCH 065/132] code cleanup --- bin/count_aln.py | 9 --------- 1 file changed, 9 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index 5ac189e5..34f764f1 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -31,15 +31,6 @@ def parse_args(): return args - -#def raw_data(input_metrics): -# #d = dict() -# d = {} -# with open(input_metrics) as f: -# rows = ( line.split('\t') for line in f ) -# d = { row[0]:row[1] for row in rows } -# return d - def raw_data(input_metrics): d = {} with open(input_metrics) as f: From b5774437b9fd8d9a25a0d8769450c1e30dad0cf4 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 00:56:59 +0000 Subject: [PATCH 066/132] add jq in Dockerfile --- containers/Dockerfile | 1 + 1 file changed, 1 insertion(+) diff --git a/containers/Dockerfile b/containers/Dockerfile index aeab904f..362ff6ee 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -27,6 +27,7 @@ RUN export DEBIAN_FRONTEND="noninteractive" && \ datamash \ graphviz \ vim \ + jq \ && rm -rf /var/lib/apt/lists/* # install conda From 6ae426e39545c4def3c5d7d7dd16a50f941d7f57 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 02:20:40 +0000 Subject: [PATCH 067/132] try key value without strip float --- bin/count_aln.py | 22 +++++++++++++++++----- 1 file changed, 17 insertions(+), 5 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index 34f764f1..1c5d0ab6 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -31,6 +31,22 @@ def parse_args(): return args +#def raw_data(input_metrics): +# d = {} +# with open(input_metrics) as f: +# for line in f: +# if not line.strip(): +# continue +# row = line.split('\t') +# key = row[0] +# value_str = row[1] +# try: +# value = float(value_str.strip()) +# except ValueError: +# value = value_str.strip() +# d[key] = value +# return d + def raw_data(input_metrics): d = {} with open(input_metrics) as f: @@ -40,11 +56,7 @@ def raw_data(input_metrics): row = line.split('\t') key = row[0] value_str = row[1] - try: - value = float(value_str.strip()) - except ValueError: - value = value_str.strip() - d[key] = value + d[key] = value_str.replace("\n", "") return d From 6c4e35d063e5ac0564f60484a6a41031b04e10f5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 02:57:59 +0000 Subject: [PATCH 068/132] test run to get key value without altering to float and log --- bin/count_aln.py | 31 ++++++++++++++++--------------- 1 file changed, 16 insertions(+), 15 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index 1c5d0ab6..7d31b6e3 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -31,21 +31,22 @@ def parse_args(): return args -#def raw_data(input_metrics): -# d = {} -# with open(input_metrics) as f: -# for line in f: -# if not line.strip(): -# continue -# row = line.split('\t') -# key = row[0] -# value_str = row[1] -# try: -# value = float(value_str.strip()) -# except ValueError: -# value = value_str.strip() -# d[key] = value -# return d +def raw_data(input_metrics): + d = {} + with open(input_metrics) as f: + for line in f: + if not line.strip(): + continue + row = line.split('\t') + key = row[0] + value_str = row[1] + d[key] = value_str.replace("\n", "") + #try: + # value = float(value_str.strip()) + #except ValueError: + # value = value_str.strip() + #d[key] = value + return d def raw_data(input_metrics): d = {} From 00fa3e7fc1cd33f463e91c312861f50d6402ea42 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 05:16:18 +0000 Subject: [PATCH 069/132] test update key value --- bin/count_aln.py | 12 ------------ 1 file changed, 12 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index 7d31b6e3..dc0f1fdd 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -48,18 +48,6 @@ def raw_data(input_metrics): #d[key] = value return d -def raw_data(input_metrics): - d = {} - with open(input_metrics) as f: - for line in f: - if not line.strip(): - continue - row = line.split('\t') - key = row[0] - value_str = row[1] - d[key] = value_str.replace("\n", "") - return d - def save_output(data_metrics, outfile): with open(outfile, "w") as f: From 87a25a18d2b17a4afbfc0bf3de927c78fca40505 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 06:59:28 +0000 Subject: [PATCH 070/132] remove space and add tab in awk print --- modules/CollectMultipleMetrics/main.nf | 2 +- modules/CollectWgsMetrics/main.nf | 6 +++--- modules/samtools/main.nf | 8 ++++---- modules/verifybamid2/main.nf | 2 +- 4 files changed, 9 insertions(+), 9 deletions(-) diff --git a/modules/CollectMultipleMetrics/main.nf b/modules/CollectMultipleMetrics/main.nf index 84c2548c..b8021759 100644 --- a/modules/CollectMultipleMetrics/main.nf +++ b/modules/CollectMultipleMetrics/main.nf @@ -30,6 +30,6 @@ process picard_collect_multiple_metrics { METRIC_ACCUMULATION_LEVEL=null \ METRIC_ACCUMULATION_LEVEL=ALL_READS - cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30\t", \$1}' > "${sample}.quality_yield_metrics.metrics" + cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30\t",\$1}' > "${sample}.quality_yield_metrics.metrics" """ } diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index 02fdec83..34175c68 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -23,9 +23,9 @@ process picard_collect_wgs_metrics { INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT - cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage", \$1}' > "${sample}_wgs_metrics.metrics" - cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x", \$1}' >> "${sample}_wgs_metrics.metrics" - cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage", \$1}' >> "${sample}_wgs_metrics.metrics" + cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t",\$1}' > "${sample}_wgs_metrics.metrics" + cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t",\$1}' >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t",\$1}' >> "${sample}_wgs_metrics.metrics" # cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 63d79e5b..943c00f1 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -23,13 +23,13 @@ process samtools_stats { --threads ${task.cpus} \\ > "${sample}.stats" - grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size", \$1}' >"${sample}.samtools.metrics" - grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t", \$1}' >>"${sample}.samtools.metrics" - grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t", \$1}' >>"${sample}.samtools.metrics" + grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size\t",\$1}' >"${sample}.samtools.metrics" + grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t",\$1}' >>"${sample}.samtools.metrics" + grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t",\$1}' >>"${sample}.samtools.metrics" # grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) - div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t %.2f", 100 * num / denom) }') + div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t%.2f",100 * num / denom) }') echo \$div >>"${sample}.samtools.metrics" """ } diff --git a/modules/verifybamid2/main.nf b/modules/verifybamid2/main.nf index 7546624b..5fa10bbb 100644 --- a/modules/verifybamid2/main.nf +++ b/modules/verifybamid2/main.nf @@ -23,7 +23,7 @@ process verifybamid2 { verifybamid2 --NumPC 4 --NumThread ${task.cpus*2} --SVDPrefix ${params.vbi2_svdprefix} ${reference} --BamFile ${bam} --Output ${sample} - cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate\t", \$1}' > "${sample}.selfSM.metrics" + cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate\t",\$1}' > "${sample}.selfSM.metrics" """ } From eef9ed607da391e3b4a27689413ab819a83cc10d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 07:01:20 +0000 Subject: [PATCH 071/132] code update to print key value --- containers/Dockerfile | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index 362ff6ee..c3b9d4c1 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -51,7 +51,9 @@ RUN git clone -b feature/rework-aln-variant-metrics-json https://github.com/c-BI # RUN git clone https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin/multiqc_plugins -# RUN git checkout feature/rework-aln-variant-metrics-json -RUN python3 setup.py install +RUN git checkout feature/rework-aln-variant-metrics-json + +## RUN python3 setup.py install + # RUN python -m pip install . -# RUN pip3 install numpy scipy argparse matplotlib +RUN pip3 install numpy scipy argparse matplotlib From 408403f5e8966f847d65231538eeeef72b1f9df1 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Fri, 30 Aug 2024 08:14:23 +0000 Subject: [PATCH 072/132] replace space in count_aln nf --- modules/CollectMultipleMetrics/main.nf | 2 +- modules/CollectWgsMetrics/main.nf | 6 +++--- modules/count_aln/main.nf | 1 + modules/samtools/main.nf | 8 ++++---- modules/verifybamid2/main.nf | 2 +- 5 files changed, 10 insertions(+), 9 deletions(-) diff --git a/modules/CollectMultipleMetrics/main.nf b/modules/CollectMultipleMetrics/main.nf index b8021759..90f827b7 100644 --- a/modules/CollectMultipleMetrics/main.nf +++ b/modules/CollectMultipleMetrics/main.nf @@ -30,6 +30,6 @@ process picard_collect_multiple_metrics { METRIC_ACCUMULATION_LEVEL=null \ METRIC_ACCUMULATION_LEVEL=ALL_READS - cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30\t",\$1}' > "${sample}.quality_yield_metrics.metrics" + cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30","\t",\$1}' > "${sample}.quality_yield_metrics.metrics" """ } diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index 34175c68..4d50ab6c 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -23,9 +23,9 @@ process picard_collect_wgs_metrics { INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT - cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t",\$1}' > "${sample}_wgs_metrics.metrics" - cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t",\$1}' >> "${sample}_wgs_metrics.metrics" - cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t",\$1}' >> "${sample}_wgs_metrics.metrics" + cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage","\t",\$1}' > "${sample}_wgs_metrics.metrics" + cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x","\t",\$1}' >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage","\t",\$1}' >> "${sample}_wgs_metrics.metrics" # cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/count_aln/main.nf b/modules/count_aln/main.nf index 158ef942..8baa2c75 100644 --- a/modules/count_aln/main.nf +++ b/modules/count_aln/main.nf @@ -10,6 +10,7 @@ process count_aln { """ cat ${stats} ${picard_quality} ${picard_wgs_coverage} ${verifybamid_freemix} > "${sample}.aln.list.txt" + sed -i 's/ //' "${sample}.aln.list.txt" count_aln.py \ --input_metrics "${sample}.aln.list.txt" \ diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 943c00f1..0d69a486 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -23,13 +23,13 @@ process samtools_stats { --threads ${task.cpus} \\ > "${sample}.stats" - grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size\t",\$1}' >"${sample}.samtools.metrics" - grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t",\$1}' >>"${sample}.samtools.metrics" - grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t",\$1}' >>"${sample}.samtools.metrics" + grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size","\t",\$1}' >"${sample}.samtools.metrics" + grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation","\t",\$1}' >>"${sample}.samtools.metrics" + grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired","\t",\$1}' >>"${sample}.samtools.metrics" # grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) - div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("pct_reads_mapped\t%.2f",100 * num / denom) }') + div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%s\t %.2f", "pct_reads_mapped", 100 * num / denom) }') echo \$div >>"${sample}.samtools.metrics" """ } diff --git a/modules/verifybamid2/main.nf b/modules/verifybamid2/main.nf index 5fa10bbb..62a6c279 100644 --- a/modules/verifybamid2/main.nf +++ b/modules/verifybamid2/main.nf @@ -23,7 +23,7 @@ process verifybamid2 { verifybamid2 --NumPC 4 --NumThread ${task.cpus*2} --SVDPrefix ${params.vbi2_svdprefix} ${reference} --BamFile ${bam} --Output ${sample} - cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate\t",\$1}' > "${sample}.selfSM.metrics" + cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate","\t",\$1}' > "${sample}.selfSM.metrics" """ } From 49265e7c6469a6e7b145d542387f9d7b6af2fe09 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 2 Sep 2024 02:02:06 +0000 Subject: [PATCH 073/132] combine the aln and vcf metrics --- bin/compile_metrics.py.UPDATE | 91 ----------- bin/count_variants.py.BAK | 239 ---------------------------- bin/count_variants.py.UPDATE | 289 ---------------------------------- modules/count_aln_vcf/main.nf | 10 +- 4 files changed, 8 insertions(+), 621 deletions(-) delete mode 100755 bin/compile_metrics.py.UPDATE delete mode 100755 bin/count_variants.py.BAK delete mode 100755 bin/count_variants.py.UPDATE diff --git a/bin/compile_metrics.py.UPDATE b/bin/compile_metrics.py.UPDATE deleted file mode 100755 index e60a3bb1..00000000 --- a/bin/compile_metrics.py.UPDATE +++ /dev/null @@ -1,91 +0,0 @@ -#!/usr/bin/env python3 - -import logging -import argparse -import json -import metrics - - -def parse_args(): - parser = argparse.ArgumentParser() - parser.add_argument("--multiqc_json", dest="multiqc_json", required=True, - default=None, - help="Path to multiqc_data.json.") - parser.add_argument("--output_json", dest="output_json", required=False, - default="./metrics.json", - help="Path to output json. Default: ./metrics.json") - parser.add_argument("--biosample_id", dest="biosample_id", required=True, - default=None, - help="Biosample ID. Default: None") - parser.add_argument("--loglevel", dest="loglevel", required=False, - default="INFO", - help="Set logging level to INFO (default), WARNING or DEBUG.") - args = parser.parse_args() - return args - - -def set_logging(loglevel): - numeric_level = getattr(logging, loglevel.upper(), None) - if not isinstance(numeric_level, int): - raise ValueError('Invalid log level: %s' % loglevel) - logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', - level=numeric_level) - - -def load_multi_qc(multiqc_json): - logging.info("Reading multiqc output: %s", multiqc_json) - with open(multiqc_json) as json_file: - data_metrics = json.load(json_file) - # keep raw data to simplify parsing downstream - mqc = data_metrics["report_saved_raw_data"] - return mqc - - -def calculate_metrics(mqc): - metrics_list = [ - # primary metrics - "yield_bp_q30", - # alignment - "pct_reads_mapped", "pct_reads_properly_paired", - # coverage - "mean_autosome_coverage", "mad_autosome_coverage", - "pct_autosomes_15x", - # insert size - "mean_insert_size", - "insert_size_std_deviation", - "cross_contamination_rate", - # variant calling - "count_snvs", "ratio_heterozygous_homzygous_snv", - "ratio_heterozygous_homzygous_indel", - "count_deletions", "count_insertions", "ratio_insertion_deletion", - "ratio_heterozygous_homzygous_insertion_deletion", "ratio_transitions_transversions" - ] - result = dict() - logging.info("Calculating %d metrics..." % len(metrics_list)) - for m in metrics_list: - k, v = eval("metrics." + m + "(mqc, args.biosample_id)") - result[k] = v - - return result - - -def save_output(data_metrics, outfile): - with open(outfile, "w") as f: - data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} - json.dump(data_metrics, f, sort_keys=True, indent=4) - f.write("\n") - - -def done(): - logging.info("DONE") - - -if __name__ == "__main__": - args = parse_args() - set_logging(args.loglevel) - - mqc = load_multi_qc(args.multiqc_json) - parsed_metrics = calculate_metrics(mqc) - save_output(parsed_metrics, args.output_json) - - done() diff --git a/bin/count_variants.py.BAK b/bin/count_variants.py.BAK deleted file mode 100755 index bcb0e263..00000000 --- a/bin/count_variants.py.BAK +++ /dev/null @@ -1,239 +0,0 @@ -#!/usr/bin/env python3 - -import logging -import argparse -import json -import pprint as pp -import subprocess -import numpy as np -import sys -import os -from pathlib import Path - - -def parse_args(): - parser = argparse.ArgumentParser() - parser.add_argument("--input_vcf", dest="input_vcf", required=True, - default=None, - help="Path to input VCF/gVCF.") - parser.add_argument("--regions", dest="regions", required=True, - default=None, - help="Path to input autosomes_non_gap_regions_bed.") - parser.add_argument("--output_json", dest="output_json", required=False, - default="./variant_counts.json", - help="Path to output file for variant metrics. Default: ./variant_counts.json") - parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, - default="./", - help="Path to scratch dir. Default: ./") - parser.add_argument("--keep_scratch", dest="keep_scratch", required=False, - default=False, action="store_true", - help="Keep scratch dir. Default: False.") - parser.add_argument("--loglevel", dest="loglevel", required=False, - default="INFO", - help="Set logging level to INFO (default), WARNING or DEBUG.") - args = parser.parse_args() - - # create scratch dir if it doesn't exist - Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) - - return args - - -def set_logging(loglevel): - numeric_level = getattr(logging, loglevel.upper(), None) - if not isinstance(numeric_level, int): - raise ValueError('Invalid log level: %s' % loglevel) - logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', - level=numeric_level) - - -def setup(input_vcf, scratch_dir): - - # subset input vcf to include only variant sites to speed up execution from gvcf - logging.info("Subsetting variant sites from input vcf...") - variants_vcf = "%s/variants.vcf.gz" % scratch_dir - cmd = "bcftools view" - cmd += " -e 'GT=\"./.\" | GT=\".|.\" | GT=\".\" | GT=\"0/0\" | GT=\"0|0\" | GT=\"0\"'" - cmd += " -O z -o %s %s" % (variants_vcf, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.run(cmd, shell=True) - - return variants_vcf - - -def count_variants(input_vcf, scratch_dir, regions): - - # initialise results dict - r = dict() - metrics_list = [ - "all_snps", - "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", - "all_indels", - "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", - "pass_del", "pass_ins", "pass_ins_del", - "pass_snp_ts_tv" - ] - for m in metrics_list: - r[m] = 0 - - # run bcftools stats - used to obtain ts/tv and sanity checks - logging.info("Running bcftools stats for all variants...") - cmd = "tabix -p vcf %s" % input_vcf - cmd1 = "bcftools stats -R %s %s > %s/all.bcftools_stats" % (regions, input_vcf, scratch_dir) - logging.debug("CMD: %s" % cmd) - logging.debug("CMD: %s" % cmd1) - p = subprocess.run(cmd, shell=True) - p = subprocess.run(cmd1, shell=True) - - logging.info("Running bcftools stats for PASS variants...") - cmd = "bcftools stats -f PASS -R %s %s > %s/pass.bcftools_stats" % (regions, input_vcf, scratch_dir) - logging.debug("CMD: %s" % cmd) - p = subprocess.run(cmd, shell=True) - - # calculate metrics - logging.info("Counting all_snps...") - cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_snps...") - cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_het_snps...") - cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_homalt_snps...") - cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_snps"] = int(p.stdout.read()) - - logging.info("Calculating pass_snp_het_hom...") - snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) - r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) - - logging.info("Counting all_indels...") - cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_indels...") - cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_het_indels...") - cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_homalt_indels...") - cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_indels"] = int(p.stdout.read()) - - logging.info("Calculating pass_indel_het_hom...") - indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) - r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) - - - logging.info("Counting pass_del, pass_ins...") - cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" - # if length(ALT) < length(REF), label as DEL - cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - - logging.info("Calculating pass_ins_del...") - ins_del = np.divide(r["pass_ins"], r["pass_del"]) - r["pass_ins_del"] = np.round(ins_del, 2) - - logging.info("Calculating pass_snp_ts_tv...") - cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - l = p.stdout.read().decode("utf-8").strip().split("\t") - r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) - - # sanity checks - logging.info("Running sanity checks for variant counts...") - - error_count = 0 - cmd = "cat %s/all.bcftools_stats | grep '^SN'" % scratch_dir - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - - for l in p.stdout.read().splitlines(): - l = l.decode("utf-8").strip().split("\t") - k = str(l[2]).lower() - v = int(l[3]) - if "snps" in k and v != r["all_snps"]: - logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) - error_count += 1 - elif "indels" in k and v != r["all_indels"]: - logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) - error_count += 1 - - if error_count > 0: - sys.exit("Sanity checks failed, aborting execution") - - return r - - -def save_output(d, outfile): - with open(outfile, "w") as f: - json.dump(d, f, sort_keys=True, indent=4) - f.write("\n") - - -def done(scratch_dir): - if not args.keep_scratch: - # clean up - logging.info("Cleaning up...") - os.remove("%s/variants.vcf.gz" % scratch_dir) - os.remove("%s/variants.vcf.gz.tbi" % scratch_dir) - os.remove("%s/all.bcftools_stats" % scratch_dir) - os.remove("%s/pass.bcftools_stats" % scratch_dir) - if len(os.listdir(scratch_dir)) == 0: - os.rmdir(scratch_dir) - else: - logging.warning("Couldn't remove scratch dir (%s): directory is not empty" % scratch_dir) - logging.info("DONE") - - -if __name__ == "__main__": - args = parse_args() - set_logging(args.loglevel) - - variants_vcf = setup(args.input_vcf, args.scratch_dir) - - vcf_metrics = count_variants(variants_vcf, args.scratch_dir, args.regions) - save_output(vcf_metrics, args.output_json) - - done(args.scratch_dir) diff --git a/bin/count_variants.py.UPDATE b/bin/count_variants.py.UPDATE deleted file mode 100755 index 00e894b4..00000000 --- a/bin/count_variants.py.UPDATE +++ /dev/null @@ -1,289 +0,0 @@ -#!/usr/bin/env python3 - -import logging -import argparse -import json -import pprint as pp -import subprocess -import numpy as np -import sys -import os -from pathlib import Path - - -def parse_args(): - parser = argparse.ArgumentParser() - parser.add_argument("--input_vcf", dest="input_vcf", required=True, - default=None, - help="Path to input VCF/gVCF.") - parser.add_argument("--regions", dest="regions", required=True, - default=None, - help="Path to input autosomes_non_gap_regions_bed.") - parser.add_argument("--output_json", dest="output_json", required=False, - default="./variant_counts.json", - help="Path to output file for variant metrics. Default: ./variant_counts.json") - parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, - default="./", - help="Path to scratch dir. Default: ./") - parser.add_argument("--keep_scratch", dest="keep_scratch", required=False, - default=False, action="store_true", - help="Keep scratch dir. Default: False.") - parser.add_argument("--loglevel", dest="loglevel", required=False, - default="INFO", - help="Set logging level to INFO (default), WARNING or DEBUG.") - args = parser.parse_args() - - # create scratch dir if it doesn't exist - Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) - - return args - - -def set_logging(loglevel): - numeric_level = getattr(logging, loglevel.upper(), None) - if not isinstance(numeric_level, int): - raise ValueError('Invalid log level: %s' % loglevel) - logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', - level=numeric_level) - - -def setup(input_vcf, scratch_dir): - - # subset input vcf to include only variant sites to speed up execution from gvcf - logging.info("Subsetting variant sites from input vcf...") - variants_vcf = "%s/variants.vcf.gz" % scratch_dir - cmd = "bcftools view" - cmd += " -e 'GT=\"./.\" | GT=\".|.\" | GT=\".\" | GT=\"0/0\" | GT=\"0|0\" | GT=\"0\"'" - cmd += " -O z -o %s %s" % (variants_vcf, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.run(cmd, shell=True) - - return variants_vcf - - -def count_variants(input_vcf, scratch_dir, regions): - - # initialise results dict - r = dict() - metrics_list = [ - "all_snps", - "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", - "all_indels", - "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", - "pass_ins_del_het_hom", "pass_del", "pass_ins", "pass_ins_del", - "pass_snp_ts_tv" - ] - for m in metrics_list: - r[m] = 0 - - # run bcftools stats - used to obtain ts/tv and sanity checks - logging.info("Running bcftools stats for all variants...") - cmd = "tabix -p vcf %s" % input_vcf - cmd1 = "bcftools stats -R %s %s > %s/all.bcftools_stats" % (regions, input_vcf, scratch_dir) - logging.debug("CMD: %s" % cmd) - logging.debug("CMD: %s" % cmd1) - p = subprocess.run(cmd, shell=True) - p = subprocess.run(cmd1, shell=True) - - logging.info("Running bcftools stats for PASS variants...") - cmd = "bcftools stats -f PASS -R %s %s > %s/pass.bcftools_stats" % (regions, input_vcf, scratch_dir) - logging.debug("CMD: %s" % cmd) - p = subprocess.run(cmd, shell=True) - - # calculate metrics - logging.info("Counting all_snps...") - cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_snps...") - cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_het_snps...") - cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_snps"] = int(p.stdout.read()) - - logging.info("Counting pass_homalt_snps...") - cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_snps"] = int(p.stdout.read()) - - logging.info("Calculating pass_snp_het_hom...") - snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) - r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) - - logging.info("Counting all_indels...") - cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_indels...") - cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_het_indels...") - cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_het_indels"] = int(p.stdout.read()) - - logging.info("Counting pass_homalt_indels...") - cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_homalt_indels"] = int(p.stdout.read()) - - logging.info("Calculating pass_indel_het_hom...") - indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) - r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) - - - logging.info("Counting pass_del_het, pass_ins_het...") - cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS_HET - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HET\" }" - # if length(ALT) < length(REF), label as DEL_HET - cmd += " else if ( length($5) < length($4) ) { print \"DEL_HET\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - - logging.info("Counting pass_del_hom, pass_ins_hom...") - cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS_HOM - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS_HOM\" }" - # if length(ALT) < length(REF), label as DEL_HOM - cmd += " else if ( length($5) < length($4) ) { print \"DEL_HOM\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - - logging.info("Counting pass_del, pass_ins...") - cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" - # if length(ALT) < length(REF), label as DEL - cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - - logging.info("Calculating pass_ins_del_het_hom...") - ins_del_het_hom = np.divide(np.sum(r["pass_ins_het"], r["pass_del_het"]), np.sum(r["pass_ins_hom"], r["pass_del_hom"])) - r["pass_ins_del_het_hom"] = np.round(ins_del_het_hom, 2) - - logging.info("Calculating pass_ins_del...") - ins_del = np.divide(r["pass_ins"], r["pass_del"]) - r["pass_ins_del"] = np.round(ins_del, 2) - - logging.info("Calculating pass_snp_ts_tv...") - cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - l = p.stdout.read().decode("utf-8").strip().split("\t") - r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) - - # sanity checks - logging.info("Running sanity checks for variant counts...") - - error_count = 0 - cmd = "cat %s/all.bcftools_stats | grep '^SN'" % scratch_dir - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - - for l in p.stdout.read().splitlines(): - l = l.decode("utf-8").strip().split("\t") - k = str(l[2]).lower() - v = int(l[3]) - if "snps" in k and v != r["all_snps"]: - logging.error("Mismatch in SNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_snps"])) - error_count += 1 - elif "indels" in k and v != r["all_indels"]: - logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) - error_count += 1 - - if error_count > 0: - sys.exit("Sanity checks failed, aborting execution") - - return r - - -def save_output(d, outfile): - with open(outfile, "w") as f: - json.dump(d, f, sort_keys=True, indent=4) - f.write("\n") - - -def done(scratch_dir): - if not args.keep_scratch: - # clean up - logging.info("Cleaning up...") - os.remove("%s/variants.vcf.gz" % scratch_dir) - os.remove("%s/variants.vcf.gz.tbi" % scratch_dir) - os.remove("%s/all.bcftools_stats" % scratch_dir) - os.remove("%s/pass.bcftools_stats" % scratch_dir) - if len(os.listdir(scratch_dir)) == 0: - os.rmdir(scratch_dir) - else: - logging.warning("Couldn't remove scratch dir (%s): directory is not empty" % scratch_dir) - logging.info("DONE") - - -if __name__ == "__main__": - args = parse_args() - set_logging(args.loglevel) - - variants_vcf = setup(args.input_vcf, args.scratch_dir) - - vcf_metrics = count_variants(variants_vcf, args.scratch_dir, args.regions) - save_output(vcf_metrics, args.output_json) - - done(args.scratch_dir) diff --git a/modules/count_aln_vcf/main.nf b/modules/count_aln_vcf/main.nf index 34d7094d..9dc63a42 100644 --- a/modules/count_aln_vcf/main.nf +++ b/modules/count_aln_vcf/main.nf @@ -6,9 +6,15 @@ process count_aln_vcf { tuple val(sample), path(count_aln), path(count_variants) output: - tuple val(sample), path("${sample}.aln.variant.metrics.json"), emit: metrics + tuple val(sample), path("${sample}.metrics.json"), emit: metrics """ - cat ${count_aln} ${count_variants} > "${sample}.aln.variant.metrics.json" + # cat ${count_aln} ${count_variants} > "${sample}.metrics.json" + + compile_aln_variants_metrics.py \ + --input_aln_metrics ${count_aln} \ + --input_variants_metrics ${count_variants} \ + --sample_id ${sample} \ + --output_json ${sample}.metrics.json """ } From b56a74167af172f094389b0966c8e6967fd4ec8e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 04:54:47 +0000 Subject: [PATCH 074/132] code upde count_aln and compile_aln_variants --- containers/Dockerfile | 5 +++-- modules/CollectMultipleMetrics/main.nf | 2 +- modules/CollectWgsMetrics/main.nf | 6 +++--- modules/count_aln/main.nf | 2 +- modules/samtools/main.nf | 12 +++++++----- modules/verifybamid2/main.nf | 2 +- 6 files changed, 16 insertions(+), 13 deletions(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index c3b9d4c1..72610eb8 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -49,11 +49,12 @@ RUN conda clean -a RUN git clone -b feature/rework-aln-variant-metrics-json https://github.com/c-BIG/NPM-sample-qc.git # RUN git clone https://github.com/c-BIG/NPM-sample-qc.git -WORKDIR /NPM-sample-qc/bin/multiqc_plugins +# WORKDIR /NPM-sample-qc/bin/multiqc_plugins +WORKDIR /NPM-sample-qc/bin RUN git checkout feature/rework-aln-variant-metrics-json ## RUN python3 setup.py install # RUN python -m pip install . -RUN pip3 install numpy scipy argparse matplotlib +RUN pip3 install numpy scipy argparse diff --git a/modules/CollectMultipleMetrics/main.nf b/modules/CollectMultipleMetrics/main.nf index 90f827b7..21c330cf 100644 --- a/modules/CollectMultipleMetrics/main.nf +++ b/modules/CollectMultipleMetrics/main.nf @@ -30,6 +30,6 @@ process picard_collect_multiple_metrics { METRIC_ACCUMULATION_LEVEL=null \ METRIC_ACCUMULATION_LEVEL=ALL_READS - cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30","\t",\$1}' > "${sample}.quality_yield_metrics.metrics" + cut -f9 "${sample}.quality_yield_metrics.txt" | grep "PF_Q30_BASES" -A 1 | grep -v "PF_Q30_BASES" | awk '{print "yield_bp_q30\t"\$1}' > "${sample}.quality_yield_metrics.metrics" """ } diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index 4d50ab6c..d6860ffa 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -23,9 +23,9 @@ process picard_collect_wgs_metrics { INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT - cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage","\t",\$1}' > "${sample}_wgs_metrics.metrics" - cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x","\t",\$1}' >> "${sample}_wgs_metrics.metrics" - cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage","\t",\$1}' >> "${sample}_wgs_metrics.metrics" + cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t"\$1}' > "${sample}_wgs_metrics.metrics" + cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t"\$1}' >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" # cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/count_aln/main.nf b/modules/count_aln/main.nf index 8baa2c75..50f9b5af 100644 --- a/modules/count_aln/main.nf +++ b/modules/count_aln/main.nf @@ -15,6 +15,6 @@ process count_aln { count_aln.py \ --input_metrics "${sample}.aln.list.txt" \ --sample_id ${sample} \ - --output_json ${sample}.aln_metrics.json + --output_json ${sample}.aln.metrics.json """ } diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 0d69a486..1f42a857 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -23,13 +23,15 @@ process samtools_stats { --threads ${task.cpus} \\ > "${sample}.stats" - grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size","\t",\$1}' >"${sample}.samtools.metrics" - grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation","\t",\$1}' >>"${sample}.samtools.metrics" - grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired","\t",\$1}' >>"${sample}.samtools.metrics" + grep "insert size average:" "${sample}.stats" |cut -f3| awk '{print "mean_insert_size\t"\$1}' >"${sample}.samtools.metrics" + grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t"\$1}' >>"${sample}.samtools.metrics" + grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t"\$1}' >>"${sample}.samtools.metrics" + # grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" + seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) - div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%s\t %.2f", "pct_reads_mapped", 100 * num / denom) }') - echo \$div >>"${sample}.samtools.metrics" + div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%s\t %.2f", "pct_reads_mapped",100 * num / denom) }') + #echo \$div >>"${sample}.samtools.metrics" """ } diff --git a/modules/verifybamid2/main.nf b/modules/verifybamid2/main.nf index 62a6c279..3cca6b0c 100644 --- a/modules/verifybamid2/main.nf +++ b/modules/verifybamid2/main.nf @@ -23,7 +23,7 @@ process verifybamid2 { verifybamid2 --NumPC 4 --NumThread ${task.cpus*2} --SVDPrefix ${params.vbi2_svdprefix} ${reference} --BamFile ${bam} --Output ${sample} - cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate","\t",\$1}' > "${sample}.selfSM.metrics" + cut -f7 "${sample}.selfSM" | grep "FREEMIX" -A 1 | grep -v "FREEMIX" | awk '{print "cross_contamination_rate\t"\$1}' > "${sample}.selfSM.metrics" """ } From 4cce7c309bcc2ee2e48d2054b3e8bf2aed6a50c9 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 04:55:40 +0000 Subject: [PATCH 075/132] code upde count_aln and compile_aln_variants --- bin/compile_aln_variants_metrics.py | 69 +++++++++++++++++++++++++++++ 1 file changed, 69 insertions(+) create mode 100644 bin/compile_aln_variants_metrics.py diff --git a/bin/compile_aln_variants_metrics.py b/bin/compile_aln_variants_metrics.py new file mode 100644 index 00000000..7af3d498 --- /dev/null +++ b/bin/compile_aln_variants_metrics.py @@ -0,0 +1,69 @@ +#!/usr/bin/env python3 + +import argparse +import json +import pprint as pp +import subprocess +import numpy as np +import sys +import os +from pathlib import Path + +def parse_args(): + parser = argparse.ArgumentParser() + parser.add_argument("--sample_id", dest="sample_id", required=True, + default=None, + help="Sample ID") + parser.add_argument("--input_aln_metrics", dest="input_aln_metrics", required=True, + default=None, + help="Path to input aln metrics list") + parser.add_argument("--input_variants_metrics", dest="input_variants_metrics", required=True, + default=None, + help="Path to input variants metrics list") + parser.add_argument("--output_json", dest="output_json", required=False, + default="./metrics.json", + help="Path to output file for variant metrics. Default: ./variant_counts.json") + parser.add_argument("--scratch_dir", dest="scratch_dir", required=False, + default="./", + help="Path to scratch dir. Default: ./") + args = parser.parse_args() + + # create scratch dir if it doesn't exist + Path(args.scratch_dir).mkdir(parents=True, exist_ok=True) + + return args + + +#with open("variant_counts.json", 'r') as f1: +# data1 = json.load(f1) + +#with open("NA12878-chr14-AKT1.variant.metrics.json", 'r') as f2: +# data2 = json.load(f2) + +#with open("merged_output_json", "w") as f: +# json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) +# f.write("\n") + +def data1(input_aln_metrics): + aln_input = {} + with open(input_aln_metrics, 'r') as f1: + aln_input = json.load(f1) + return aln_input + +def data2(input_variants_metrics): + variants_input = {} + with open(input_variants_metrics, 'r') as f2: + variants_input = json.load(f2) + return variants_input + +def save_output(data1, data2, outfile): + with open(outfile, "w") as f: + json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) + f.write("\n") + +if __name__ == "__main__": + args = parse_args() + + aln = data1(args.input_aln_metrics) + variants = data2(args.input_variants_metrics) + save_output(aln, variants, args.output_json) From f67f69d35e431e6bf918b9a7ba0c39d6b4dfd577 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 05:03:25 +0000 Subject: [PATCH 076/132] code upde count_aln and compile_aln_variants --- bin/count_aln.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index dc0f1fdd..b07555f1 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -51,7 +51,7 @@ def raw_data(input_metrics): def save_output(data_metrics, outfile): with open(outfile, "w") as f: - data_metrics = {"sample" : {"id" : args.sample_id}, "wgs_metrics" : data_metrics} + data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : data_metrics} json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") From 947e561a590492c69433799b16d7ec2d96acab2f Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 05:06:56 +0000 Subject: [PATCH 077/132] code upde count_aln and compile_aln_variants --- bin/compile_aln_variants_metrics.py | 10 ---------- 1 file changed, 10 deletions(-) mode change 100644 => 100755 bin/compile_aln_variants_metrics.py diff --git a/bin/compile_aln_variants_metrics.py b/bin/compile_aln_variants_metrics.py old mode 100644 new mode 100755 index 7af3d498..1c7734ed --- a/bin/compile_aln_variants_metrics.py +++ b/bin/compile_aln_variants_metrics.py @@ -34,16 +34,6 @@ def parse_args(): return args -#with open("variant_counts.json", 'r') as f1: -# data1 = json.load(f1) - -#with open("NA12878-chr14-AKT1.variant.metrics.json", 'r') as f2: -# data2 = json.load(f2) - -#with open("merged_output_json", "w") as f: -# json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) -# f.write("\n") - def data1(input_aln_metrics): aln_input = {} with open(input_aln_metrics, 'r') as f1: From 4d78180f1540630fa91e15a35059542e82c0eaf3 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 06:37:05 +0000 Subject: [PATCH 078/132] remove compile_metrics.py and fix_multiqc_picard_wgsmetrics.py --- bin/{compile_metrics.py => compile_metrics.py.BAK} | 0 ..._picard_wgsmetrics.py => fix_multiqc_picard_wgsmetrics.py.BAK} | 0 2 files changed, 0 insertions(+), 0 deletions(-) rename bin/{compile_metrics.py => compile_metrics.py.BAK} (100%) rename bin/{fix_multiqc_picard_wgsmetrics.py => fix_multiqc_picard_wgsmetrics.py.BAK} (100%) diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py.BAK similarity index 100% rename from bin/compile_metrics.py rename to bin/compile_metrics.py.BAK diff --git a/bin/fix_multiqc_picard_wgsmetrics.py b/bin/fix_multiqc_picard_wgsmetrics.py.BAK similarity index 100% rename from bin/fix_multiqc_picard_wgsmetrics.py rename to bin/fix_multiqc_picard_wgsmetrics.py.BAK From 83e37cba5d2bb30b42dbf169c2a12990b4a58c49 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 06:37:49 +0000 Subject: [PATCH 079/132] rename multiqc_plugins to multiqc_plugins_BAK --- .../multiqc_npm/__init__.py | 0 bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/cli.py | 0 bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/hooks.py | 0 .../multiqc_npm/modules/__init__.py | 0 .../multiqc_npm/modules/npm_calculate_callability.py | 0 .../multiqc_npm/modules/npm_count_variants.py | 0 .../multiqc_npm/modules/npm_extra_metrics.py | 0 .../multiqc_npm/modules/npm_picard_QualityYieldMetrics.py | 0 .../multiqc_npm/modules/npm_samtools_stats_bq.py | 0 bin/{multiqc_plugins => multiqc_plugins_BAK}/setup.py | 0 10 files changed, 0 insertions(+), 0 deletions(-) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/__init__.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/cli.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/hooks.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/__init__.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/npm_calculate_callability.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/npm_count_variants.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/npm_extra_metrics.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/multiqc_npm/modules/npm_samtools_stats_bq.py (100%) rename bin/{multiqc_plugins => multiqc_plugins_BAK}/setup.py (100%) diff --git a/bin/multiqc_plugins/multiqc_npm/__init__.py b/bin/multiqc_plugins_BAK/multiqc_npm/__init__.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/__init__.py rename to bin/multiqc_plugins_BAK/multiqc_npm/__init__.py diff --git a/bin/multiqc_plugins/multiqc_npm/cli.py b/bin/multiqc_plugins_BAK/multiqc_npm/cli.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/cli.py rename to bin/multiqc_plugins_BAK/multiqc_npm/cli.py diff --git a/bin/multiqc_plugins/multiqc_npm/hooks.py b/bin/multiqc_plugins_BAK/multiqc_npm/hooks.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/hooks.py rename to bin/multiqc_plugins_BAK/multiqc_npm/hooks.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/__init__.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/__init__.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_calculate_callability.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/npm_calculate_callability.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/npm_count_variants.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/npm_extra_metrics.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py diff --git a/bin/multiqc_plugins/multiqc_npm/modules/npm_samtools_stats_bq.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py similarity index 100% rename from bin/multiqc_plugins/multiqc_npm/modules/npm_samtools_stats_bq.py rename to bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py diff --git a/bin/multiqc_plugins/setup.py b/bin/multiqc_plugins_BAK/setup.py similarity index 100% rename from bin/multiqc_plugins/setup.py rename to bin/multiqc_plugins_BAK/setup.py From 2fcbb2e1a1b9e160a13ec260e8dce8571729eb5d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 06:45:06 +0000 Subject: [PATCH 080/132] rename module compile_metrics and multiqc --- modules/{compile_metrics => compile_metrics_BAK}/main.nf | 0 modules/{multiqc => multiqc_BAK}/main.nf | 0 2 files changed, 0 insertions(+), 0 deletions(-) rename modules/{compile_metrics => compile_metrics_BAK}/main.nf (100%) rename modules/{multiqc => multiqc_BAK}/main.nf (100%) diff --git a/modules/compile_metrics/main.nf b/modules/compile_metrics_BAK/main.nf similarity index 100% rename from modules/compile_metrics/main.nf rename to modules/compile_metrics_BAK/main.nf diff --git a/modules/multiqc/main.nf b/modules/multiqc_BAK/main.nf similarity index 100% rename from modules/multiqc/main.nf rename to modules/multiqc_BAK/main.nf From 2c680d5c3a5a2e994848caa4db45bd0c3ffef42e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 07:04:37 +0000 Subject: [PATCH 081/132] update print aln metrics tab issue --- modules/samtools/main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 1f42a857..2fa71bd7 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -31,7 +31,7 @@ process samtools_stats { seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) - div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%s\t %.2f", "pct_reads_mapped",100 * num / denom) }') - #echo \$div >>"${sample}.samtools.metrics" + div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%.2f", 100 * num / denom) }') + echo "pct_reads_mapped\t"\$div >>"${sample}.samtools.metrics" """ } From 6424cce383784adf63ac4eb2b0be0244dad85699 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 07:05:32 +0000 Subject: [PATCH 082/132] comment include/multiqc module --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 700b6efe..74c933cb 100644 --- a/main.nf +++ b/main.nf @@ -97,7 +97,7 @@ include { bcftools_stats } from './modules/bcftools' include { count_variants } from './modules/count_variants' include { count_aln } from './modules/count_aln' include { count_aln_vcf } from './modules/count_aln_vcf' -include { multiqc } from './modules/multiqc' +// include { multiqc } from './modules/multiqc' /* ---------------------------------------------------------------------- From 7d31de52bf62866f7f540e157c4f73ef55a6bb37 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 07:56:08 +0000 Subject: [PATCH 083/132] update variants metric key names --- bin/count_variants.py | 30 +++++++++++++++--------------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/bin/count_variants.py b/bin/count_variants.py index 47d8a35c..ca74af3c 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -69,10 +69,10 @@ def count_variants(input_vcf, scratch_dir, regions): # initialise results dict r = dict() metrics_list = [ - "pass_snps", "pass_snp_het_hom", - "pass_indel_het_hom", - "pass_del", "pass_ins", "pass_ins_del", - "pass_snp_ts_tv" + "count_snvs", "ratio_heterozygous_homzygous_snv", + "ratio_heterozygous_homzygous_indel", + "count_deletions", "count_insertions", "ratio_insertion_deletion", + "ratio_transitions_transversions_snv" ] for m in metrics_list: r[m] = 0 @@ -99,11 +99,11 @@ def count_variants(input_vcf, scratch_dir, regions): # r["all_snps"] = int(p.stdout.read()) all_snps = int(p.stdout.read()) - logging.info("Counting pass_snps...") + logging.info("Counting count_snvs...") cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_snps"] = int(p.stdout.read()) + r["count_snvs"] = int(p.stdout.read()) logging.info("Counting pass_het_snps...") cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) @@ -120,10 +120,10 @@ def count_variants(input_vcf, scratch_dir, regions): pass_homalt_snps = int(p.stdout.read()) - logging.info("Calculating pass_snp_het_hom...") + logging.info("Calculating ratio_heterozygous_homzygous_snv...") # snp_het_hom = np.divide(r["pass_het_snps"], r["pass_homalt_snps"]) snp_het_hom = np.divide(pass_het_snps, pass_homalt_snps) - r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) + r["ratio_heterozygous_homzygous_snv"] = np.round(snp_het_hom, 2) logging.info("Counting all_indels...") cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) @@ -153,13 +153,13 @@ def count_variants(input_vcf, scratch_dir, regions): # r["pass_homalt_indels"] = int(p.stdout.read()) pass_homalt_indels = int(p.stdout.read()) - logging.info("Calculating pass_indel_het_hom...") + logging.info("Calculating ratio_heterozygous_homzygous_indel...") # indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) indel_het_hom = np.divide(pass_het_indels, pass_homalt_indels) - r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) + r["ratio_heterozygous_homzygous_indel"] = np.round(indel_het_hom, 2) - logging.info("Counting pass_del, pass_ins...") + logging.info("Counting count_deletions, count_insertions...") cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) # filter dragen annotation of variant records cmd += " | sed 's/,//g'" @@ -182,16 +182,16 @@ def count_variants(input_vcf, scratch_dir, regions): v = int(l[0]) r[k] = v - logging.info("Calculating pass_ins_del...") - ins_del = np.divide(r["pass_ins"], r["pass_del"]) - r["pass_ins_del"] = np.round(ins_del, 2) + logging.info("Calculating ratio_insertion_deletion...") + ins_del = np.divide(r["count_insertions"], r["count_deletions"]) + r["ratio_insertion_deletion"] = np.round(ins_del, 2) logging.info("Calculating pass_snp_ts_tv...") cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) l = p.stdout.read().decode("utf-8").strip().split("\t") - r["pass_snp_ts_tv"] = np.round(float(l[4]), 2) + r["ratio_transitions_transversions_snv"] = np.round(float(l[4]), 2) # sanity checks logging.info("Running sanity checks for variant counts...") From c2d5e6786b97aba36ec9d3606d81af5353dc6b33 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:01:15 +0000 Subject: [PATCH 084/132] files cleanup --- bin/compile_metrics.py.BAK | 91 - bin/fix_multiqc_picard_wgsmetrics.py.BAK | 39 - main.nf.BAK | 277 + modules/compile_metrics_BAK/main.nf | 21 - modules/multiqc_BAK/main.nf | 28 - .../.samtools.metrics | 2 + .../output_bak/pipeline_info/dag.pdf | Bin 0 -> 18211 bytes .../output_bak/pipeline_info/report.html | 1044 ++ .../output_bak/pipeline_info/timeline.html | 224 + .../output_bak/pipeline_info/trace.txt | 4 + .../bcftools/NA12878-chr14-AKT1.pass.stats | 1805 +++ .../NA12878-chr14-AKT1.variant_counts.json | 16 + .../multiqc/NA12878-chr14-AKT1.metrics.json | 23 + .../multiqc_data/multiqc.log | 28 + .../multiqc_data/multiqc_bcftools_stats.json | 31 + .../multiqc_data/multiqc_citations.json | 8 + .../multiqc_data/multiqc_data.json | 11707 ++++++++++++++++ .../multiqc_data/multiqc_general_stats.json | 9 + .../multiqc_npm_count_variants.json | 18 + .../multiqc_software_versions.json | 10 + .../multiqc_data/multiqc_sources.json | 7 + .../NA12878-chr14-AKT1/multiqc_report.html | 5200 +++++++ .../params.yaml.BAK | 5 + .../run.sh | 3 +- .../NA12878.vc_metrics.csv | 25 + .../params.yaml | 1 - .../params.yaml.BAK | 6 + .../params.yaml.aln | 4 + .../params.yaml.aln-vcf | 5 + .../params.yaml.vcf | 4 + 30 files changed, 20464 insertions(+), 181 deletions(-) delete mode 100755 bin/compile_metrics.py.BAK delete mode 100644 bin/fix_multiqc_picard_wgsmetrics.py.BAK create mode 100644 main.nf.BAK delete mode 100644 modules/compile_metrics_BAK/main.nf delete mode 100644 modules/multiqc_BAK/main.nf create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/dag.pdf create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/report.html create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_citations.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_data.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_general_stats.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_npm_count_variants.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_software_versions.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_sources.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_report.html create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf diff --git a/bin/compile_metrics.py.BAK b/bin/compile_metrics.py.BAK deleted file mode 100755 index fdc7d5e4..00000000 --- a/bin/compile_metrics.py.BAK +++ /dev/null @@ -1,91 +0,0 @@ -#!/usr/bin/env python3 - -import logging -import argparse -import json -import metrics - - -def parse_args(): - parser = argparse.ArgumentParser() - parser.add_argument("--multiqc_json", dest="multiqc_json", required=True, - default=None, - help="Path to multiqc_data.json.") - parser.add_argument("--output_json", dest="output_json", required=False, - default="./metrics.json", - help="Path to output json. Default: ./metrics.json") - parser.add_argument("--biosample_id", dest="biosample_id", required=True, - default=None, - help="Biosample ID. Default: None") - parser.add_argument("--loglevel", dest="loglevel", required=False, - default="INFO", - help="Set logging level to INFO (default), WARNING or DEBUG.") - args = parser.parse_args() - return args - - -def set_logging(loglevel): - numeric_level = getattr(logging, loglevel.upper(), None) - if not isinstance(numeric_level, int): - raise ValueError('Invalid log level: %s' % loglevel) - logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', - level=numeric_level) - - -def load_multi_qc(multiqc_json): - logging.info("Reading multiqc output: %s", multiqc_json) - with open(multiqc_json) as json_file: - data_metrics = json.load(json_file) - # keep raw data to simplify parsing downstream - mqc = data_metrics["report_saved_raw_data"] - return mqc - - -def calculate_metrics(mqc): - metrics_list = [ - # primary metrics - "yield_bp_q30", - # alignment - "pct_reads_mapped", "pct_reads_properly_paired", - # coverage - "mean_autosome_coverage", "mad_autosome_coverage", - "pct_autosomes_15x", - # insert size - "mean_insert_size", - "insert_size_std_deviation", - "cross_contamination_rate", - # variant calling - "count_snvs", "ratio_heterozygous_homzygous_snv", - "ratio_heterozygous_homzygous_indel", - "count_deletions", "count_insertions", "ratio_insertion_deletion", - "ratio_transitions_transversions" - ] - result = dict() - logging.info("Calculating %d metrics..." % len(metrics_list)) - for m in metrics_list: - k, v = eval("metrics." + m + "(mqc, args.biosample_id)") - result[k] = v - - return result - - -def save_output(data_metrics, outfile): - with open(outfile, "w") as f: - data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} - json.dump(data_metrics, f, sort_keys=True, indent=4) - f.write("\n") - - -def done(): - logging.info("DONE") - - -if __name__ == "__main__": - args = parse_args() - set_logging(args.loglevel) - - mqc = load_multi_qc(args.multiqc_json) - parsed_metrics = calculate_metrics(mqc) - save_output(parsed_metrics, args.output_json) - - done() diff --git a/bin/fix_multiqc_picard_wgsmetrics.py.BAK b/bin/fix_multiqc_picard_wgsmetrics.py.BAK deleted file mode 100644 index ccad4379..00000000 --- a/bin/fix_multiqc_picard_wgsmetrics.py.BAK +++ /dev/null @@ -1,39 +0,0 @@ -#!/usr/bin/env python3 - -import argparse -import logging - - -def parse_args(): - parser = argparse.ArgumentParser() - parser.add_argument("--input", dest="input", required=True, - default=None, - help="Path to picard CollectWgsMetrics file.") - args = parser.parse_args() - return args - - -# Replace metrics class name -# This temporary fix is needed due to compatibility issue with GATK -# 4.1.2.0 and MultiQC 1.8. MultiQC searches for CollectWgsMetrics -# instead of WgsMetrics in the report file. Note that this should be -# resolved in the 1.9 release of MultiQC. -# From https://github.com/genialis/resolwe-bio/blob/master/resolwe_bio/processes/support_processors/wgs_metrics.py#L19 -def replace_metrics_class(fname): - with open(fname, 'r') as report: - newlines = [] - for line in report.readlines(): - if line == '## METRICS CLASS\tpicard.analysis.WgsMetrics\n': - line = '## METRICS CLASS\tCollectWgsMetrics$WgsMetrics\n' - newlines.append(line) - else: - newlines.append(line) - with open(fname, 'w') as report: - for line in newlines: - report.writelines(line) - - -if __name__ == "__main__": - args = parse_args() - replace_metrics_class(args.input) - print("DONE: %s" % args.input) diff --git a/main.nf.BAK b/main.nf.BAK new file mode 100644 index 00000000..81dd44e0 --- /dev/null +++ b/main.nf.BAK @@ -0,0 +1,277 @@ +#!/usr/bin/env nextflow + +import groovy.yaml.YamlSlurper + +nextflow.enable.dsl=2 + +/* +Developed by the Genome Institute of Singapore for +the National Precision Medicine Programme + +Copyright: 2022 Genome Institute of Singapore +License: The MIT License (MIT) + +See LICENSE for more copyright information +*/ + +/* +---------------------------------------------------------------------- +FUNCTIONS +---------------------------------------------------------------------- +*/ + +def helpMessage() { + log.info """ + Usage: nextflow run main.nf -config nextflow.config -params-file params.yaml + -work-dir ./ --outdir ./ + [-resume] [--keep_workdir] [--help] + + Options: + -config Generic workflow settings + -params-file Sample-specific settings + -profile Job launch settings + -resume Re-use existing results (optional, omit to re-run from scratch) + --keep_workdir Keep work directory (optional, omit for auto-deletion) + --help Print this help message + """.stripIndent() +} + +def nextflowMessage() { + log.info "N E X T F L O W ~ version ${workflow.nextflow.version} ${workflow.nextflow.build}" +} + +def version_message() { + println("NPM-sample-qc ~ version ${workflow.manifest.version}") +} + +def minimalInformationMessage() { + log.info "User name : " + workflow.userName + log.info "Command Line : " + workflow.commandLine + log.info "Project Dir : " + workflow.projectDir + log.info "Launch Dir : " + workflow.launchDir + log.info "Work Dir : " + workflow.workDir + log.info "Results Dir : " + params.publish_dir + log.info "Info Dir : " + params.info_dir + log.info "Profile : " + workflow.profile +} + +def startMessage() { + this.nextflowMessage() + this.version_message() + this.minimalInformationMessage() +} + + +/* +---------------------------------------------------------------------- +USAGE +---------------------------------------------------------------------- +*/ + +if (params.help) exit 0, helpMessage() + +/* +---------------------------------------------------------------------- +LAUNCH INFO +---------------------------------------------------------------------- +*/ + +startMessage() + +/* +---------------------------------------------------------------------- +PROCESSES +--------------------------------------------------------------------- +*/ + +include { samtools_stats as samtools_stats_bam } from './modules/samtools' +include { samtools_stats as samtools_stats_cram } from './modules/samtools' +include { verifybamid2 as verifybamid2_bam } from './modules/verifybamid2' +include { verifybamid2 as verifybamid2_cram } from './modules/verifybamid2' +include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_bam } from './modules/CollectMultipleMetrics' +include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_cram } from './modules/CollectMultipleMetrics' +include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_bam } from './modules/CollectWgsMetrics' +include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from './modules/CollectWgsMetrics' +include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' +include { bcftools_stats } from './modules/bcftools' +include { count_variants } from './modules/count_variants' +include { multiqc } from './modules/multiqc' + +/* +---------------------------------------------------------------------- +WORKFLOW +--------------------------------------------------------------------- +*/ + +// main + +workflow { + + ref_fasta = file( params.reference ) + ref_fasta_idx = file( params.reference + ".fai" ) + autosomes_non_gap_regions = file( params.autosomes_non_gap_regions ) + autosomes_non_gap_regions_bed = file( params.autosomes_non_gap_regions_bed ) + vbi2_ud = file( params.vbi2_ud ) + vbi2_bed = file( params.vbi2_bed ) + vbi2_mean = file( params.vbi2_mean ) + //ref_dbsnp = file( params.ref_dbsnp ) + + inputs = new YamlSlurper().parse(file(params.inputs_list)) + + Channel + .fromList(inputs['samples']) + .ifEmpty { ['biosample_id': params.biosample_id, 'aln': params.aln] } + .set { samples } + + Channel + samples.branch { rec -> + def aln_file = rec.aln ? file( rec.aln ) : null + + bam: rec.biosample_id && aln_file?.extension == 'bam' + def bam_idx = file( "${rec.aln}.bai" ) + + return tuple( rec.biosample_id, aln_file, bam_idx ) + + cram: rec.biosample_id && aln_file?.extension == 'cram' + def cram_idx = file( "${rec.aln}.crai" ) + + return tuple( rec.biosample_id, aln_file, cram_idx ) + } + .set { aln_inputs } + + + samtools_stats_bam( aln_inputs.bam, [] ) + samtools_stats_cram( aln_inputs.cram, ref_fasta ) + + verifybamid2_bam( aln_inputs.bam, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) + verifybamid2_cram( aln_inputs.cram, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) + + picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) + picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) + + picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) + + + Channel + samples.branch { rec -> + def vcf_file = rec.vcf ? file( rec.vcf ) : null + + output: rec.biosample_id && vcf_file + def vcf_idx = file( "${rec.vcf}.tbi" ) + + return tuple( rec.biosample_id, vcf_file, vcf_idx ) + } + .set { vcf_inputs } + + bcftools_stats( vcf_inputs ) + //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) + count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) + + Channel + samples.map { it.biosample_id } + .set { sample_ids } + +// channel for samplelist vcf input file processed outputs + Channel + .empty() + sample_ids + .join( count_variants.out ) + //count_variants.out + .join( bcftools_stats.out ) + //.view() + .set { vcf_qc } + +// channel for samplelist input file type bam processed outputs + Channel + .empty() + sample_ids + .join( samtools_stats_bam.out.stats ) + .join( picard_collect_multiple_metrics_bam.out.insert_size ) + .join( picard_collect_multiple_metrics_bam.out.quality ) + .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) + .join( verifybamid2_bam.out.freemix, remainder: true ) + .set { ch_bam } + +// channel for samplelist input file type cram processed outputs + Channel + .empty() + sample_ids + .join( samtools_stats_cram.out.stats ) + .join( picard_collect_multiple_metrics_cram.out.insert_size ) + .join( picard_collect_multiple_metrics_cram.out.quality ) + .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) + .join( verifybamid2_cram.out.freemix, remainder: true ) + .set { ch_cram } + +/* +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + ch_bam.mix(ch_cram) + .combine(vcf_qc,by:0) + //.view() + .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } + //.view() + .set { multiqc_in } +*/ +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + ch_bam.mix(ch_cram) // .ifEmpty([]) + //.combine(vcf_qc,by:0) + .join(vcf_qc, remainder: true) + //.map { it.minus(null) } + //.map { files -> files - null } + //.flatten() + .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } + .view() + .set { multiqc_in } + + +/* +// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty + Channel + .empty() + sample_ids + //.combine(vcf_qc,by:0).ifEmpty([]) + //ch_bam.mix(ch_cram).ifEmpty([]) + .join(count_variants.out) + .join(bcftools_stats.out) + .map { sample, count_variants, bcftools_stats -> sample, count_variants, bcftools_stats } + .mix(ch_bam,ch_cram) + //.combine(vcf_qc,by:0).ifEmpty([]) + //.join(vcf_qc) + //.view() + //.mix(vcf_qc).ifEmpty([]) + //.join(vcf_qc).ifEmpty([]) + //.combine(vcf_qc,by:0).ifEmpty([]) + //.flatten() + //.toList() + .view() + //.map { sample, count_variants, bcftools_stats, sample1, stats, insertsize, quality, wgs_coverage, freemix -> [ sample ?: [], count_variants ?: [], bcftools_stats ?: [], sample1 ?: [], stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [] ] } + //.view() + .set { multiqc_in } +*/ + + multiqc( multiqc_in ) + +} + +// .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } + +/* +---------------------------------------------------------------------- +COMPLETION INFO +---------------------------------------------------------------------- +*/ +workflow.onComplete { + log.info "Started : " + workflow.start + log.info "Completed : " + workflow.complete + log.info "Duration : " + workflow.duration + log.info "Status : " + workflow.success + log.info "Publish dir : " + params.publish_dir +} + +workflow.onError { + log.info "Workflow execution stopped with the following message:" + log.info "Exit status : " + workflow.exitStatus + log.info "Error message : " + workflow.errorMessage + log.info "Error report : " + (workflow.errorReport ?: '-') +} diff --git a/modules/compile_metrics_BAK/main.nf b/modules/compile_metrics_BAK/main.nf deleted file mode 100644 index 0577d330..00000000 --- a/modules/compile_metrics_BAK/main.nf +++ /dev/null @@ -1,21 +0,0 @@ -process compile_metrics { - - tag { sample_ids } - - input: - val(sample_ids) - path "multiqc_data.json" - - output: - tuple val(sample_ids), path("${sample_ids}.metrics.json"), emit: compile_metrics_out - - script: - """ - # parse and calculate all the metrics in the multiqc output to compile - - compile_metrics.py \ - --multiqc_json multiqc_data.json \ - --output_json "${sample_ids}.metrics.json" \\ - --biosample_id "${sample_ids}" \\ - """ -} diff --git a/modules/multiqc_BAK/main.nf b/modules/multiqc_BAK/main.nf deleted file mode 100644 index 62e47a2a..00000000 --- a/modules/multiqc_BAK/main.nf +++ /dev/null @@ -1,28 +0,0 @@ -process multiqc { - - tag { sample } - - input: - // tuple val(sample), path(variantscounts), path(stats) - tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(picard_wgs_coverage), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) - // tuple val(sample), path(stats), path(picard_insert_size), path(picard_quality), path(verifybamid_freemix), path(count_variants), path(bcftools_stats) - - output: - tuple val(sample), path("${sample}/multiqc_report.html"), emit: report - tuple val(sample), path("${sample}/multiqc_data"), emit: data - tuple val(sample), path("${sample}/multiqc_data/multiqc_data.json"), emit: json_data - tuple val(sample), path("${sample}.metrics.json"), emit: compile_metrics_out - - """ - multiqc \\ - --data-format json \\ - --enable-npm-plugin \\ - -o ${sample} \\ - . - - compile_metrics.py \ - --multiqc_json "${sample}/multiqc_data/multiqc_data.json" \ - --output_json "${sample}.metrics.json" \\ - --biosample_id "${sample}" \\ - """ -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics new file mode 100644 index 00000000..57cdadea --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics @@ -0,0 +1,2 @@ +99.6307 +99.6307 diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/dag.pdf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/dag.pdf new file mode 100644 index 0000000000000000000000000000000000000000..24ccaa321cded6d12179984deb5dcd8db7bac962 GIT binary patch literal 18211 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+ +
+
+ +

Nextflow workflow report

+

[silly_lalande]

+ + +
+ Workflow execution completed successfully! +
+ + +
+
Run times
+
+ 29-May-2024 09:35:31 - 29-May-2024 09:35:45 + (duration: 13.8s) +
+ +
+
+
  3 succeeded  
+
  0 cached  
+
  0 ignored  
+
  0 failed  
+
+
+ +
Nextflow command
+ 
nextflow run ../../main.nf -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config --inputs_list params.yaml -work-dir ./work --publish_dir ./output
+
+ +
+
CPU-Hours
+
(a few seconds)
+ +
Launch directory
+
/home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6
+ +
Work directory
+
/home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work
+ +
Project directory
+
/home/ubuntu/April-7/NPM-sample-qc
+ + +
Script name
+
main.nf
+ + + +
Script ID
+
0fd4c7c6c4c6dbeb225d67dd88188176
+ + +
Workflow session
+
d3938eda-dd28-461d-a1aa-faafbd2badc3
+ + + +
Workflow profile
+
standard
+ + +
Workflow container
+
cbig/npm-sample-qc:v0.12.0
+ +
Container engine
+
docker
+ + +
Nextflow version
+
version 23.09.1-edge, build 5881 (11-09-2023 10:08 UTC)
+
+
+
+ +
+

Resource Usage

+

These plots give an overview of the distribution of resource usage for each process.

+ +

CPU

+ +
+
+
+
+
+
+
+ +
+ +

Memory

+ +
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This table shows information about each task in the workflow. Use the search box on the right + to filter rows for specific values. Clicking headers will sort the table by that value and + scrolling side to side will reveal more columns.

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+ (tasks table omitted because the dataset is too big) +
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+ Generated by Nextflow, version 23.09.1-edge +
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+ + + + + diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html new file mode 100644 index 00000000..cf0816c6 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html @@ -0,0 +1,224 @@ + + + + + + + + + + + + +
+

Processes execution timeline

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+ Launch time:
+ Elapsed time:
+ Legend: job wall time / memory usage (RAM) +

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+ + + + + + + diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt new file mode 100644 index 00000000..a56a7fb8 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt @@ -0,0 +1,4 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +2 b6/e2ba69 885662 bcftools_stats (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:34.815 3.3s 53ms 1178.9% 3.4 MB 4.7 MB 678.8 KB 38.8 KB +1 d7/181748 885992 count_variants (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:38.138 2.1s 1.4s 1108.0% 49.8 MB 698.4 MB 22.9 MB 10.5 MB +3 d4/01e073 886629 multiqc (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:40.222 5s 4.3s 148.3% 117.6 MB 866.8 MB 49.6 MB 17.7 MB diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats new file mode 100644 index 00000000..6b47284e --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats @@ -0,0 +1,1805 @@ +# This file was produced by bcftools stats (1.15.1+htslib-1.19) and can be plotted using plot-vcfstats. +# The command line was: bcftools stats -f PASS NA12878-chr14-AKT1.vcf.gz +# +# Definition of sets: +# ID [2]id [3]tab-separated file names +ID 0 NA12878-chr14-AKT1.vcf.gz +# SN, Summary numbers: +# number of records .. number of data rows in the VCF +# number of no-ALTs .. reference-only sites, ALT is either "." or identical to REF +# number of SNPs .. number of rows with a SNP +# number of MNPs .. number of rows with a MNP, such as CC>TT +# number of indels .. number of rows with an indel +# number of others .. number of rows with other type, for example a symbolic allele or +# a complex substitution, such as ACT>TCGA +# number of multiallelic sites .. number of rows with multiple alternate alleles +# number of multiallelic SNP sites .. number of rows with multiple alternate alleles, all SNPs +# +# Note that rows containing multiple types will be counted multiple times, in each +# counter. For example, a row with a SNP and an indel increments both the SNP and +# the indel counter. +# +# SN [2]id [3]key [4]value +SN 0 number of samples: 1 +SN 0 number of records: 10321 +SN 0 number of no-ALTs: 0 +SN 0 number of SNPs: 8489 +SN 0 number of MNPs: 0 +SN 0 number of indels: 1843 +SN 0 number of others: 0 +SN 0 number of multiallelic sites: 151 +SN 0 number of multiallelic SNP sites: 6 +# TSTV, transitions/transversions: +# TSTV [2]id [3]ts [4]tv [5]ts/tv [6]ts (1st ALT) [7]tv (1st ALT) [8]ts/tv (1st ALT) +TSTV 0 5784 2711 2.13 5776 2702 2.14 +# SiS, Singleton stats: +# SiS [2]id [3]allele count [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable +SiS 0 1 4347 2983 1364 1237 0 0 1237 +# AF, Stats by non-reference allele frequency: +# AF [2]id [3]allele frequency [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable +AF 0 0.000000 4347 2983 1364 1237 0 0 1237 +AF 0 0.990000 4148 2801 1347 740 0 0 740 +# QUAL, Stats by quality +# QUAL [2]id [3]Quality [4]number of SNPs [5]number of transitions (1st ALT) [6]number of transversions (1st ALT) [7]number of indels +QUAL 0 5.0 3 1 2 0 +QUAL 0 5.2 2 2 0 0 +QUAL 0 5.4 1 0 1 1 +QUAL 0 5.5 3 1 2 0 +QUAL 0 5.9 0 0 0 4 +QUAL 0 6.2 0 0 0 1 +QUAL 0 6.3 0 0 0 1 +QUAL 0 6.4 2 1 1 1 +QUAL 0 6.5 2 2 0 1 +QUAL 0 7.0 1 1 0 1 +QUAL 0 7.1 1 1 0 1 +QUAL 0 7.2 1 1 0 0 +QUAL 0 7.3 1 1 0 0 +QUAL 0 7.4 2 1 1 0 +QUAL 0 7.5 1 1 0 0 +QUAL 0 7.6 15 7 8 5 +QUAL 0 7.7 1 0 1 0 +QUAL 0 7.8 3 2 1 1 +QUAL 0 7.9 1 1 0 0 +QUAL 0 8.1 2 1 1 0 +QUAL 0 8.4 1 1 0 0 +QUAL 0 8.5 1 1 0 0 +QUAL 0 8.6 4 2 2 1 +QUAL 0 8.7 1 1 0 2 +QUAL 0 8.9 4 4 0 0 +QUAL 0 9.0 1 1 0 0 +QUAL 0 9.1 3 2 1 1 +QUAL 0 9.3 4 3 1 0 +QUAL 0 9.4 7 6 1 1 +QUAL 0 9.5 12 6 6 1 +QUAL 0 9.7 1 1 0 0 +QUAL 0 9.8 1 0 1 0 +QUAL 0 9.9 2 1 1 0 +QUAL 0 10.2 1 0 1 0 +QUAL 0 10.3 1 1 0 0 +QUAL 0 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0 0 +QUAL 0 501.8 1 0 1 0 +QUAL 0 506.6 0 0 0 1 +QUAL 0 513.0 0 0 0 1 +QUAL 0 516.2 0 0 0 1 +QUAL 0 528.2 1 0 1 0 +QUAL 0 530.5 1 1 0 0 +QUAL 0 535.7 0 0 0 1 +QUAL 0 557.5 1 1 0 0 +QUAL 0 558.0 1 1 0 0 +QUAL 0 578.3 0 0 0 1 +QUAL 0 579.4 1 0 1 0 +QUAL 0 591.2 1 1 0 0 +QUAL 0 591.3 1 1 0 0 +QUAL 0 599.6 1 1 0 0 +QUAL 0 605.6 1 0 1 0 +QUAL 0 605.8 1 1 0 0 +QUAL 0 612.9 1 1 0 0 +QUAL 0 615.9 1 1 0 0 +QUAL 0 627.2 1 0 1 0 +QUAL 0 629.8 1 1 0 0 +QUAL 0 631.0 1 1 0 0 +QUAL 0 648.8 1 0 1 0 +QUAL 0 650.9 1 1 0 0 +QUAL 0 685.3 1 1 0 0 +QUAL 0 772.6 1 1 0 0 +QUAL 0 843.7 0 0 0 1 +# IDD, InDel distribution: +# IDD [2]id [3]length (deletions negative) [4]number of sites [5]number of genotypes [6]mean VAF +IDD 0 -60 6 0 . +IDD 0 -59 1 0 . +IDD 0 -56 1 0 . +IDD 0 -54 1 0 . +IDD 0 -50 1 0 . +IDD 0 -47 1 0 . +IDD 0 -42 1 0 . +IDD 0 -41 2 0 . +IDD 0 -40 2 0 . +IDD 0 -38 1 0 . +IDD 0 -36 2 0 . +IDD 0 -31 2 0 . +IDD 0 -30 1 0 . +IDD 0 -28 2 0 . +IDD 0 -27 4 0 . +IDD 0 -26 2 0 . +IDD 0 -25 3 0 . +IDD 0 -24 3 0 . +IDD 0 -22 1 0 . +IDD 0 -21 5 0 . +IDD 0 -20 8 0 . +IDD 0 -18 5 0 . +IDD 0 -17 3 0 . +IDD 0 -16 5 0 . +IDD 0 -15 7 0 . +IDD 0 -14 8 0 . +IDD 0 -13 7 0 . +IDD 0 -12 16 0 . +IDD 0 -11 4 0 . +IDD 0 -10 11 0 . +IDD 0 -9 6 0 . +IDD 0 -8 28 0 . +IDD 0 -7 9 0 . +IDD 0 -6 28 0 . +IDD 0 -5 31 0 . +IDD 0 -4 97 0 . +IDD 0 -3 67 0 . +IDD 0 -2 155 0 . +IDD 0 -1 430 0 . +IDD 0 1 437 0 . +IDD 0 2 181 0 . +IDD 0 3 82 0 . +IDD 0 4 95 0 . +IDD 0 5 24 0 . +IDD 0 6 30 0 . +IDD 0 7 11 0 . +IDD 0 8 34 0 . +IDD 0 9 7 0 . +IDD 0 10 19 0 . +IDD 0 11 2 0 . +IDD 0 12 16 0 . +IDD 0 13 3 0 . +IDD 0 14 7 0 . +IDD 0 15 4 0 . +IDD 0 16 7 0 . +IDD 0 17 1 0 . +IDD 0 18 2 0 . +IDD 0 19 2 0 . +IDD 0 20 3 0 . +IDD 0 22 2 0 . +IDD 0 24 5 0 . +IDD 0 26 1 0 . +IDD 0 27 1 0 . +IDD 0 28 1 0 . +IDD 0 30 1 0 . +IDD 0 32 1 0 . +IDD 0 33 3 0 . +IDD 0 34 1 0 . +IDD 0 36 3 0 . +IDD 0 38 1 0 . +IDD 0 40 2 0 . +IDD 0 41 2 0 . +IDD 0 42 3 0 . +IDD 0 44 1 0 . +IDD 0 46 1 0 . +IDD 0 59 1 0 . +IDD 0 60 13 0 . +# ST, Substitution types: +# ST [2]id [3]type [4]count +ST 0 A>C 319 +ST 0 A>G 1404 +ST 0 A>T 230 +ST 0 C>A 329 +ST 0 C>G 464 +ST 0 C>T 1505 +ST 0 G>A 1455 +ST 0 G>C 458 +ST 0 G>T 331 +ST 0 T>A 255 +ST 0 T>C 1420 +ST 0 T>G 325 +# DP, Depth distribution +# DP [2]id [3]bin [4]number of genotypes [5]fraction of genotypes (%) [6]number of sites [7]fraction of sites (%) +DP 0 2 0 0.000000 106 1.027032 +DP 0 3 0 0.000000 110 1.065788 +DP 0 4 0 0.000000 76 0.736363 +DP 0 5 0 0.000000 71 0.687918 +DP 0 6 0 0.000000 60 0.581339 +DP 0 7 0 0.000000 48 0.465071 +DP 0 8 0 0.000000 48 0.465071 +DP 0 9 0 0.000000 68 0.658851 +DP 0 10 0 0.000000 61 0.591028 +DP 0 11 0 0.000000 83 0.804186 +DP 0 12 0 0.000000 92 0.891386 +DP 0 13 0 0.000000 111 1.075477 +DP 0 14 0 0.000000 83 0.804186 +DP 0 15 0 0.000000 116 1.123922 +DP 0 16 0 0.000000 116 1.123922 +DP 0 17 0 0.000000 134 1.298324 +DP 0 18 0 0.000000 118 1.143300 +DP 0 19 0 0.000000 134 1.298324 +DP 0 20 0 0.000000 169 1.637438 +DP 0 21 0 0.000000 152 1.472726 +DP 0 22 0 0.000000 168 1.627749 +DP 0 23 0 0.000000 171 1.656816 +DP 0 24 0 0.000000 196 1.899041 +DP 0 25 0 0.000000 238 2.305978 +DP 0 26 0 0.000000 245 2.373801 +DP 0 27 0 0.000000 289 2.800116 +DP 0 28 0 0.000000 344 3.333010 +DP 0 29 0 0.000000 382 3.701192 +DP 0 30 0 0.000000 365 3.536479 +DP 0 31 0 0.000000 385 3.730259 +DP 0 32 0 0.000000 412 3.991861 +DP 0 33 0 0.000000 395 3.827149 +DP 0 34 0 0.000000 397 3.846526 +DP 0 35 0 0.000000 436 4.224397 +DP 0 36 0 0.000000 417 4.040306 +DP 0 37 0 0.000000 422 4.088751 +DP 0 38 0 0.000000 383 3.710881 +DP 0 39 0 0.000000 338 3.274876 +DP 0 40 0 0.000000 305 2.955140 +DP 0 41 0 0.000000 272 2.635404 +DP 0 42 0 0.000000 248 2.402868 +DP 0 43 0 0.000000 227 2.199399 +DP 0 44 0 0.000000 168 1.627749 +DP 0 45 0 0.000000 180 1.744017 +DP 0 46 0 0.000000 135 1.308013 +DP 0 47 0 0.000000 107 1.036721 +DP 0 48 0 0.000000 107 1.036721 +DP 0 49 0 0.000000 75 0.726674 +DP 0 50 0 0.000000 76 0.736363 +DP 0 51 0 0.000000 72 0.697607 +DP 0 52 0 0.000000 57 0.552272 +DP 0 53 0 0.000000 41 0.397248 +DP 0 54 0 0.000000 44 0.426315 +DP 0 55 0 0.000000 42 0.406937 +DP 0 56 0 0.000000 26 0.251914 +DP 0 57 0 0.000000 21 0.203469 +DP 0 58 0 0.000000 19 0.184091 +DP 0 59 0 0.000000 21 0.203469 +DP 0 60 0 0.000000 18 0.174402 +DP 0 61 0 0.000000 9 0.087201 +DP 0 62 0 0.000000 9 0.087201 +DP 0 63 0 0.000000 14 0.135646 +DP 0 64 0 0.000000 8 0.077512 +DP 0 65 0 0.000000 10 0.096890 +DP 0 66 0 0.000000 14 0.135646 +DP 0 67 0 0.000000 9 0.087201 +DP 0 68 0 0.000000 4 0.038756 +DP 0 69 0 0.000000 2 0.019378 +DP 0 70 0 0.000000 1 0.009689 +DP 0 71 0 0.000000 2 0.019378 +DP 0 72 0 0.000000 2 0.019378 +DP 0 73 0 0.000000 3 0.029067 +DP 0 74 0 0.000000 3 0.029067 +DP 0 75 0 0.000000 4 0.038756 +DP 0 76 0 0.000000 1 0.009689 +DP 0 78 0 0.000000 2 0.019378 +DP 0 79 0 0.000000 3 0.029067 +DP 0 80 0 0.000000 1 0.009689 +DP 0 81 0 0.000000 1 0.009689 +DP 0 82 0 0.000000 1 0.009689 +DP 0 84 0 0.000000 1 0.009689 +DP 0 87 0 0.000000 2 0.019378 +DP 0 90 0 0.000000 1 0.009689 +DP 0 95 0 0.000000 1 0.009689 +DP 0 104 0 0.000000 1 0.009689 +DP 0 107 0 0.000000 1 0.009689 +DP 0 109 0 0.000000 3 0.029067 +DP 0 110 0 0.000000 2 0.019378 +DP 0 111 0 0.000000 2 0.019378 +DP 0 112 0 0.000000 3 0.029067 +DP 0 113 0 0.000000 1 0.009689 diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json new file mode 100644 index 00000000..4e78482d --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json @@ -0,0 +1,16 @@ +{ + "all_indels": 1860, + "all_snps": 8512, + "pass_del": 848, + "pass_het_indels": 1103, + "pass_het_snps": 4341, + "pass_homalt_indels": 740, + "pass_homalt_snps": 4148, + "pass_indel_het_hom": 1.49, + "pass_indels": 1843, + "pass_ins": 850, + "pass_ins_del": 1.0, + "pass_snp_het_hom": 1.05, + "pass_snp_ts_tv": 2.13, + "pass_snps": 8489 +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json new file mode 100644 index 00000000..d97f88d2 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json @@ -0,0 +1,23 @@ +{ + "biosample": { + "id": "NA12878-chr14-AKT1" + }, + "wgs_qc_metrics": { + "count_deletions": 848, + "count_insertions": 850, + "count_snvs": 8489, + "cross_contamination_rate": "NA", + "insert_size_std_deviation": "NA", + "mad_autosome_coverage": "NA", + "mean_autosome_coverage": "NA", + "mean_insert_size": "NA", + "pct_autosomes_15x": "NA", + "pct_reads_mapped": "NA", + "pct_reads_properly_paired": "NA", + "ratio_heterozygous_homzygous_indel": 1.49, + "ratio_heterozygous_homzygous_snv": 1.05, + "ratio_insertion_deletion": 1.0, + "ratio_transitions_transversions": 2.13, + "yield_bp_q30": "NA" + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log new file mode 100644 index 00000000..81d40fde --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log @@ -0,0 +1,28 @@ +[2024-05-29 09:35:42,298] multiqc [DEBUG ] This is MultiQC v1.21 +[2024-05-29 09:35:42,298] multiqc [INFO ] Expanded search patterns with the following: multiqc_npm/picard_quality_yield_metrics, multiqc_npm/samtools_stats_bq, multiqc_npm/count_variants +[2024-05-29 09:35:42,299] multiqc [DEBUG ] Command used: /usr/local/conda/bin/multiqc --data-format json --enable-npm-plugin -o NA12878-chr14-AKT1 . +[2024-05-29 09:35:43,009] multiqc [WARNING] MultiQC Version v1.22.1 now available! +[2024-05-29 09:35:43,009] multiqc [DEBUG ] Latest MultiQC version is v1.22.1, released 2024-05-17 +[2024-05-29 09:35:43,009] multiqc [DEBUG ] Working dir : /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191 +[2024-05-29 09:35:43,009] multiqc [DEBUG ] Template : default +[2024-05-29 09:35:43,009] multiqc [DEBUG ] Running Python 3.12.2 | packaged by conda-forge | (main, Feb 16 2024, 20:50:58) [GCC 12.3.0] +[2024-05-29 09:35:43,010] multiqc [DEBUG ] Analysing modules: multiqc_npm, custom_content, ccs, ngsderive, purple, conpair, isoseq, lima, peddy, somalier, methylqa, mosdepth, phantompeakqualtools, qualimap, bamdst, preseq, hifiasm, quast, qorts, rna_seqc, rockhopper, rsem, rseqc, busco, bustools, goleft_indexcov, gffcompare, disambiguate, supernova, deeptools, sargasso, verifybamid, mirtrace, happy, mirtop, sambamba, gopeaks, homer, hops, macs2, theta2, snpeff, gatk, htseq, bcftools, featureCounts, fgbio, dragen, dragen_fastqc, dedup, pbmarkdup, damageprofiler, mapdamage, biobambam2, jcvi, mtnucratio, picard, vep, bakta, prokka, qc3C, nanostat, samblaster, samtools, sexdeterrmine, seqera_cli, eigenstratdatabasetools, bamtools, jellyfish, vcftools, longranger, stacks, varscan2, snippy, umitools, truvari, megahit, bbmap, bismark, biscuit, diamond, hicexplorer, hicup, hicpro, salmon, kallisto, slamdunk, star, hisat2, tophat, bowtie2, bowtie1, cellranger, snpsplit, odgi, pangolin, nextclade, freyja, humid, kat, leehom, librarian, nonpareil, adapterRemoval, bbduk, clipandmerge, cutadapt, flexbar, sourmash, kaiju, bracken, kraken, malt, motus, trimmomatic, sickle, skewer, sortmerna, biobloomtools, fastq_screen, afterqc, fastp, fastqc, filtlong, prinseqplusplus, pychopper, porechop, pycoqc, minionqc, anglerfish, multivcfanalyzer, clusterflow, checkqc, bcl2fastq, bclconvert, interop, ivar, flash, seqyclean, optitype, whatshap, spaceranger, xenome, xengsort, metaphlan, seqwho +[2024-05-29 09:35:43,010] multiqc [DEBUG ] Using temporary directory for creating report: /tmp/tmp_6i2nysw +[2024-05-29 09:35:43,010] multiqc [INFO ] Search path : /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191 +[2024-05-29 09:35:43,050] multiqc [DEBUG ] Summary of files that were skipped by the search: [skipped_module_specific_max_filesize: 16] // [skipped_no_match: 7] +[2024-05-29 09:35:44,035] multiqc [INFO ] Running with MultiQC NPM plugin +[2024-05-29 09:35:44,036] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_npm_count_variants.json +[2024-05-29 09:35:44,036] multiqc [INFO ] Found 1 npm_count_variants reports +[2024-05-29 09:35:44,187] multiqc [DEBUG ] No samples found: custom_content +[2024-05-29 09:35:44,194] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_bcftools_stats.json +[2024-05-29 09:35:44,301] multiqc.modules.bcftools.bcftools [INFO ] Found 1 stats reports +[2024-05-29 09:35:44,301] multiqc.utils.software_versions [DEBUG ] Reading software versions from config.software_versions +[2024-05-29 09:35:44,302] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_software_versions.json +[2024-05-29 09:35:44,312] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_general_stats.json +[2024-05-29 09:35:44,313] multiqc [DEBUG ] Compressing plot data +[2024-05-29 09:35:44,356] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_data.json +[2024-05-29 09:35:44,356] multiqc [INFO ] Report : NA12878-chr14-AKT1/multiqc_report.html +[2024-05-29 09:35:44,356] multiqc [INFO ] Data : NA12878-chr14-AKT1/multiqc_data +[2024-05-29 09:35:44,356] multiqc [DEBUG ] Moving data file from '/tmp/tmp_6i2nysw/multiqc_data' to '/home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191/NA12878-chr14-AKT1/multiqc_data' +[2024-05-29 09:35:44,358] multiqc [DEBUG ] Full report path: /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191/NA12878-chr14-AKT1/multiqc_report.html +[2024-05-29 09:35:44,466] multiqc [INFO ] MultiQC complete diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json new file mode 100644 index 00000000..df7371bb --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json @@ -0,0 +1,31 @@ +{ + "NA12878-chr14-AKT1": { + 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Stats_mqc-generalstats-bcftools_stats-tstv": 2.13, + "Bcftools: Stats_mqc-generalstats-bcftools_stats-number_of_MNPs": 0 + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_npm_count_variants.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_npm_count_variants.json new file mode 100644 index 00000000..cb15a53b --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_npm_count_variants.json @@ -0,0 +1,18 @@ +{ + "NA12878-chr14-AKT1.variant": { + "all_indels": 1860, + "all_snps": 8512, + "pass_del": 848, + "pass_het_indels": 1103, + "pass_het_snps": 4341, + "pass_homalt_indels": 740, + "pass_homalt_snps": 4148, + "pass_indel_het_hom": 1.49, + "pass_indels": 1843, + "pass_ins": 850, + "pass_ins_del": 1.0, + "pass_snp_het_hom": 1.05, + "pass_snp_ts_tv": 2.13, + "pass_snps": 8489 + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_software_versions.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_software_versions.json new file mode 100644 index 00000000..8063f02e --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_software_versions.json @@ -0,0 +1,10 @@ +{ + "Bcftools": { + "Bcftools": [ + "1.15.1" + ], + "HTSlib": [ + "1.19" + ] + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_sources.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_sources.json new file mode 100644 index 00000000..fa0d9076 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_sources.json @@ -0,0 +1,7 @@ +{ + "Bcftools": { + "stats": { + "NA12878-chr14-AKT1": "/home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191/NA12878-chr14-AKT1.pass.stats" + } + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_report.html b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_report.html new file mode 100644 index 00000000..c61a63ad --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_report.html @@ -0,0 +1,5200 @@ + + + + + + + + + + + + + +MultiQC Report + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
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        + MultiQC: Summarize analysis results for multiple tools and samples in a single report
        + Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        + Bioinformatics (2016)
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        + A modular tool to aggregate results from bioinformatics analyses across many samples into a single report. +

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        Report + + generated on 2024-05-29, 09:35 UTC + + + based on data in: + + /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191

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        Sample NameVarsSNPIndelTs/Tv
        NA12878-chr14-AKT1
        10321
        8489
        1843
        2.13
        + + +
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        + + +
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        +

        Bcftools

        + +
        + + + Version: 1.15.1 + + + + HTSlib: 1.19 + + +
        + +
        +

        Bcftools contains utilities for variant calling and manipulating VCFs and BCFs.DOI: 10.1093/gigascience/giab008.

        + + + + +
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        + Variant Substitution Types + +

        + + + + +
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        Created with MultiQC
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        + Variant Quality + +

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        +
        Created with MultiQC
        +
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        + Indel Distribution + +

        + + + + +
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        + + + +
        +
        + + +
        +
        +
        Created with MultiQC
        +
        + + +
        +
        + + + + + + +
        + +

        + Variant depths + +

        + +

        Read depth support distribution for called variants

        + + +
        +
        + + + +
        +
        + + +
        +
        +
        Created with MultiQC
        +
        + + + +
        + + + +
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        + + + +
        + + +
        +
        +

        Software Versions

        + +
        +

        Software Versions lists versions of software tools extracted from file contents.

        + + + + +
        + + + + +
        + + + + + + + + + + +
        GroupSoftwareVersion
        BcftoolsBcftools1.15.1
        HTSlib1.19
        + + +
        + + +
        + + +
        + + + + +
        + +
        +
        + + +

        + + + MultiQC v1.21 + + - Written by Phil Ewels, + available on GitHub. +

        +

        + This report uses HighCharts, + jQuery, + jQuery UI, + Bootstrap, + FileSaver.js and + clipboard.js. +

        +
        +
        + + + + + + + + + + + + + + diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK new file mode 100644 index 00000000..5e6ea776 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK @@ -0,0 +1,5 @@ +samples: +- + biosample_id: NA12878-chr14-AKT1 + aln: NA12878-chr14-AKT1.bam + vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh index 610fa8ff..e4085fa4 100755 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh @@ -6,8 +6,9 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -nextflow run ../../main.nf \ +/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config \ --inputs_list params.yaml \ -work-dir ./work \ + -resume \ --publish_dir ./output diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv b/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv new file mode 100644 index 00000000..32d02fcb --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv @@ -0,0 +1,25 @@ +VARIANT CALLER SUMMARY,,Number of samples,1 +VARIANT CALLER SUMMARY,,Reads Processed,649717949 +VARIANT CALLER SUMMARY,,Child Sample,NA +VARIANT CALLER POSTFILTER,NA12878,Total,5060888,100.00 +VARIANT CALLER POSTFILTER,NA12878,Biallelic,4869445,96.22 +VARIANT CALLER POSTFILTER,NA12878,Multiallelic,191443,3.78 +VARIANT CALLER POSTFILTER,NA12878,SNPs,4078055,80.58 +VARIANT CALLER POSTFILTER,NA12878,Insertions (Hom),167967,3.32 +VARIANT CALLER POSTFILTER,NA12878,Insertions (Het),308210,6.09 +VARIANT CALLER POSTFILTER,NA12878,Deletions (Hom),164236,3.25 +VARIANT CALLER POSTFILTER,NA12878,Deletions (Het),317555,6.27 +VARIANT CALLER POSTFILTER,NA12878,Indels (Het),24626,0.49 +VARIANT CALLER POSTFILTER,NA12878,Chr X number of SNPs over genome,127260 +VARIANT CALLER POSTFILTER,NA12878,Chr Y number of SNPs over genome,0 +VARIANT CALLER POSTFILTER,NA12878,(Chr X SNPs)/(chr Y SNPs) ratio over genome,NA +VARIANT CALLER POSTFILTER,NA12878,SNP Transitions,3740035 +VARIANT CALLER POSTFILTER,NA12878,SNP Transversions,1915655 +VARIANT CALLER POSTFILTER,NA12878,Ti/Tv ratio,1.95 +VARIANT CALLER POSTFILTER,NA12878,Heterozygous,3150535 +VARIANT CALLER POSTFILTER,NA12878,Homozygous,1910353 +VARIANT CALLER POSTFILTER,NA12878,Het/Hom ratio,1.65 +VARIANT CALLER POSTFILTER,NA12878,In dbSNP,0,0.00 +VARIANT CALLER POSTFILTER,NA12878,Not in dbSNP,0,0.00 +VARIANT CALLER POSTFILTER,NA12878,Percent Callability,97.2545 +VARIANT CALLER POSTFILTER,NA12878,Percent Autosome Callability,98.1391 diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml index 2d0e3cb4..d12f9e77 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml @@ -1,5 +1,4 @@ samples: - biosample_id: NA12878 - aln: /data/NA12878.bam vcf: /data/NA12878.hard-filtered.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK new file mode 100644 index 00000000..f55939d8 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK @@ -0,0 +1,6 @@ +samples: +- + biosample_id: NA12878 + aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam + vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz +aws_no_sign_request: true diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln new file mode 100644 index 00000000..f02876a7 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln @@ -0,0 +1,4 @@ +samples: +- + biosample_id: NA12878 + aln: /data/NA12878.bam diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf new file mode 100644 index 00000000..2d0e3cb4 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf @@ -0,0 +1,5 @@ +samples: +- + biosample_id: NA12878 + aln: /data/NA12878.bam + vcf: /data/NA12878.hard-filtered.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf new file mode 100644 index 00000000..d12f9e77 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf @@ -0,0 +1,4 @@ +samples: +- + biosample_id: NA12878 + vcf: /data/NA12878.hard-filtered.vcf.gz From 41181c72edffdf0d64db1f7c249aa63aaf561268 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:01:27 +0000 Subject: [PATCH 085/132] files cleanup --- main.nf.BAK | 277 ---------------------------------------------------- 1 file changed, 277 deletions(-) delete mode 100644 main.nf.BAK diff --git a/main.nf.BAK b/main.nf.BAK deleted file mode 100644 index 81dd44e0..00000000 --- a/main.nf.BAK +++ /dev/null @@ -1,277 +0,0 @@ -#!/usr/bin/env nextflow - -import groovy.yaml.YamlSlurper - -nextflow.enable.dsl=2 - -/* -Developed by the Genome Institute of Singapore for -the National Precision Medicine Programme - -Copyright: 2022 Genome Institute of Singapore -License: The MIT License (MIT) - -See LICENSE for more copyright information -*/ - -/* ----------------------------------------------------------------------- -FUNCTIONS ----------------------------------------------------------------------- -*/ - -def helpMessage() { - log.info """ - Usage: nextflow run main.nf -config nextflow.config -params-file params.yaml - -work-dir ./ --outdir ./ - [-resume] [--keep_workdir] [--help] - - Options: - -config Generic workflow settings - -params-file Sample-specific settings - -profile Job launch settings - -resume Re-use existing results (optional, omit to re-run from scratch) - --keep_workdir Keep work directory (optional, omit for auto-deletion) - --help Print this help message - """.stripIndent() -} - -def nextflowMessage() { - log.info "N E X T F L O W ~ version ${workflow.nextflow.version} ${workflow.nextflow.build}" -} - -def version_message() { - println("NPM-sample-qc ~ version ${workflow.manifest.version}") -} - -def minimalInformationMessage() { - log.info "User name : " + workflow.userName - log.info "Command Line : " + workflow.commandLine - log.info "Project Dir : " + workflow.projectDir - log.info "Launch Dir : " + workflow.launchDir - log.info "Work Dir : " + workflow.workDir - log.info "Results Dir : " + params.publish_dir - log.info "Info Dir : " + params.info_dir - log.info "Profile : " + workflow.profile -} - -def startMessage() { - this.nextflowMessage() - this.version_message() - this.minimalInformationMessage() -} - - -/* ----------------------------------------------------------------------- -USAGE ----------------------------------------------------------------------- -*/ - -if (params.help) exit 0, helpMessage() - -/* ----------------------------------------------------------------------- -LAUNCH INFO ----------------------------------------------------------------------- -*/ - -startMessage() - -/* ----------------------------------------------------------------------- -PROCESSES ---------------------------------------------------------------------- -*/ - -include { samtools_stats as samtools_stats_bam } from './modules/samtools' -include { samtools_stats as samtools_stats_cram } from './modules/samtools' -include { verifybamid2 as verifybamid2_bam } from './modules/verifybamid2' -include { verifybamid2 as verifybamid2_cram } from './modules/verifybamid2' -include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_bam } from './modules/CollectMultipleMetrics' -include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_cram } from './modules/CollectMultipleMetrics' -include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_bam } from './modules/CollectWgsMetrics' -include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from './modules/CollectWgsMetrics' -include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' -include { bcftools_stats } from './modules/bcftools' -include { count_variants } from './modules/count_variants' -include { multiqc } from './modules/multiqc' - -/* ----------------------------------------------------------------------- -WORKFLOW ---------------------------------------------------------------------- -*/ - -// main - -workflow { - - ref_fasta = file( params.reference ) - ref_fasta_idx = file( params.reference + ".fai" ) - autosomes_non_gap_regions = file( params.autosomes_non_gap_regions ) - autosomes_non_gap_regions_bed = file( params.autosomes_non_gap_regions_bed ) - vbi2_ud = file( params.vbi2_ud ) - vbi2_bed = file( params.vbi2_bed ) - vbi2_mean = file( params.vbi2_mean ) - //ref_dbsnp = file( params.ref_dbsnp ) - - inputs = new YamlSlurper().parse(file(params.inputs_list)) - - Channel - .fromList(inputs['samples']) - .ifEmpty { ['biosample_id': params.biosample_id, 'aln': params.aln] } - .set { samples } - - Channel - samples.branch { rec -> - def aln_file = rec.aln ? file( rec.aln ) : null - - bam: rec.biosample_id && aln_file?.extension == 'bam' - def bam_idx = file( "${rec.aln}.bai" ) - - return tuple( rec.biosample_id, aln_file, bam_idx ) - - cram: rec.biosample_id && aln_file?.extension == 'cram' - def cram_idx = file( "${rec.aln}.crai" ) - - return tuple( rec.biosample_id, aln_file, cram_idx ) - } - .set { aln_inputs } - - - samtools_stats_bam( aln_inputs.bam, [] ) - samtools_stats_cram( aln_inputs.cram, ref_fasta ) - - verifybamid2_bam( aln_inputs.bam, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) - verifybamid2_cram( aln_inputs.cram, ref_fasta, vbi2_ud, vbi2_bed, vbi2_mean ) - - picard_collect_multiple_metrics_bam( aln_inputs.bam, [], [] ) - picard_collect_multiple_metrics_cram( aln_inputs.cram, ref_fasta, ref_fasta_idx ) - - picard_collect_wgs_metrics_bam( aln_inputs.bam, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - picard_collect_wgs_metrics_cram( aln_inputs.cram, autosomes_non_gap_regions, ref_fasta, ref_fasta_idx ) - - - Channel - samples.branch { rec -> - def vcf_file = rec.vcf ? file( rec.vcf ) : null - - output: rec.biosample_id && vcf_file - def vcf_idx = file( "${rec.vcf}.tbi" ) - - return tuple( rec.biosample_id, vcf_file, vcf_idx ) - } - .set { vcf_inputs } - - bcftools_stats( vcf_inputs ) - //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) - count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) - - Channel - samples.map { it.biosample_id } - .set { sample_ids } - -// channel for samplelist vcf input file processed outputs - Channel - .empty() - sample_ids - .join( count_variants.out ) - //count_variants.out - .join( bcftools_stats.out ) - //.view() - .set { vcf_qc } - -// channel for samplelist input file type bam processed outputs - Channel - .empty() - sample_ids - .join( samtools_stats_bam.out.stats ) - .join( picard_collect_multiple_metrics_bam.out.insert_size ) - .join( picard_collect_multiple_metrics_bam.out.quality ) - .join( picard_collect_wgs_metrics_bam.out.wgs_coverage ) - .join( verifybamid2_bam.out.freemix, remainder: true ) - .set { ch_bam } - -// channel for samplelist input file type cram processed outputs - Channel - .empty() - sample_ids - .join( samtools_stats_cram.out.stats ) - .join( picard_collect_multiple_metrics_cram.out.insert_size ) - .join( picard_collect_multiple_metrics_cram.out.quality ) - .join( picard_collect_wgs_metrics_cram.out.wgs_coverage ) - .join( verifybamid2_cram.out.freemix, remainder: true ) - .set { ch_cram } - -/* -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - ch_bam.mix(ch_cram) - .combine(vcf_qc,by:0) - //.view() - .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } - //.view() - .set { multiqc_in } -*/ -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - ch_bam.mix(ch_cram) // .ifEmpty([]) - //.combine(vcf_qc,by:0) - .join(vcf_qc, remainder: true) - //.map { it.minus(null) } - //.map { files -> files - null } - //.flatten() - .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } - .view() - .set { multiqc_in } - - -/* -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - Channel - .empty() - sample_ids - //.combine(vcf_qc,by:0).ifEmpty([]) - //ch_bam.mix(ch_cram).ifEmpty([]) - .join(count_variants.out) - .join(bcftools_stats.out) - .map { sample, count_variants, bcftools_stats -> sample, count_variants, bcftools_stats } - .mix(ch_bam,ch_cram) - //.combine(vcf_qc,by:0).ifEmpty([]) - //.join(vcf_qc) - //.view() - //.mix(vcf_qc).ifEmpty([]) - //.join(vcf_qc).ifEmpty([]) - //.combine(vcf_qc,by:0).ifEmpty([]) - //.flatten() - //.toList() - .view() - //.map { sample, count_variants, bcftools_stats, sample1, stats, insertsize, quality, wgs_coverage, freemix -> [ sample ?: [], count_variants ?: [], bcftools_stats ?: [], sample1 ?: [], stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [] ] } - //.view() - .set { multiqc_in } -*/ - - multiqc( multiqc_in ) - -} - -// .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } - -/* ----------------------------------------------------------------------- -COMPLETION INFO ----------------------------------------------------------------------- -*/ -workflow.onComplete { - log.info "Started : " + workflow.start - log.info "Completed : " + workflow.complete - log.info "Duration : " + workflow.duration - log.info "Status : " + workflow.success - log.info "Publish dir : " + params.publish_dir -} - -workflow.onError { - log.info "Workflow execution stopped with the following message:" - log.info "Exit status : " + workflow.exitStatus - log.info "Error message : " + workflow.errorMessage - log.info "Error report : " + (workflow.errorReport ?: '-') -} From 0ad9d93867a314d50b78adc0e6fa678218a6fb48 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:11:26 +0000 Subject: [PATCH 086/132] cleanup files and name change count_aln_vcf to compile_aln_vcf --- main.nf | 12 +++--------- modules/CollectWgsMetrics/main.nf | 1 - modules/{count_aln_vcf => compile_aln_vcf}/main.nf | 2 +- modules/samtools/main.nf | 2 -- nextflow.config | 2 +- 5 files changed, 5 insertions(+), 14 deletions(-) rename modules/{count_aln_vcf => compile_aln_vcf}/main.nf (94%) diff --git a/main.nf b/main.nf index 74c933cb..2e3dd1be 100644 --- a/main.nf +++ b/main.nf @@ -96,7 +96,7 @@ include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVa include { bcftools_stats } from './modules/bcftools' include { count_variants } from './modules/count_variants' include { count_aln } from './modules/count_aln' -include { count_aln_vcf } from './modules/count_aln_vcf' +include { compile_aln_vcf } from './modules/compile_aln_vcf' // include { multiqc } from './modules/multiqc' /* @@ -192,9 +192,7 @@ workflow { .empty() sample_ids .join( count_variants.out ) - //count_variants.out .join( bcftools_stats.out ) - //.view() .set { vcf_qc } // channel for samplelist input file type bam processed outputs @@ -226,7 +224,7 @@ workflow { count_aln ( aln_count_in ) -// channel for samplelist input file type bam processed outputs +// channel for samplelist input file type aln and vcf processed outputs Channel .empty() sample_ids @@ -236,7 +234,7 @@ workflow { .view() .set { ch_count } - count_aln_vcf ( ch_count ) + compile_aln_vcf ( ch_count ) /* // channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty @@ -296,12 +294,8 @@ workflow { } */ -// multiqc( multiqc_in ) - } -// .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } - /* ---------------------------------------------------------------------- COMPLETION INFO diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index d6860ffa..39899761 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -26,6 +26,5 @@ process picard_collect_wgs_metrics { cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t"\$1}' > "${sample}_wgs_metrics.metrics" cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t"\$1}' >> "${sample}_wgs_metrics.metrics" cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" - # cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "{mad_autosome_coverage: ", \$1,"}"}' >> "${sample}_wgs_metrics.metrics" """ } diff --git a/modules/count_aln_vcf/main.nf b/modules/compile_aln_vcf/main.nf similarity index 94% rename from modules/count_aln_vcf/main.nf rename to modules/compile_aln_vcf/main.nf index 9dc63a42..a87bd701 100644 --- a/modules/count_aln_vcf/main.nf +++ b/modules/compile_aln_vcf/main.nf @@ -1,4 +1,4 @@ -process count_aln_vcf { +process compile_aln_vcf { tag { sample } diff --git a/modules/samtools/main.nf b/modules/samtools/main.nf index 2fa71bd7..025a3644 100644 --- a/modules/samtools/main.nf +++ b/modules/samtools/main.nf @@ -27,8 +27,6 @@ process samtools_stats { grep "insert size standard deviation:" "${sample}.stats" |cut -f3| awk '{print "insert_size_std_deviation\t"\$1}' >>"${sample}.samtools.metrics" grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "pct_reads_properly_paired\t"\$1}' >>"${sample}.samtools.metrics" - # grep "percentage of properly paired reads" "${sample}.stats" |cut -f3| awk '{print "{pct_reads_properly_paired: ", \$1,"}"}' >>"${sample}.samtools.metrics" - seq=\$(grep " sequences:" "${sample}.stats" |cut -f3) mapped_reads=\$(grep " reads mapped:" "${sample}.stats" |cut -f3) div=\$(awk -v num=\$mapped_reads -v denom=\$seq 'BEGIN { printf ("%.2f", 100 * num / denom) }') diff --git a/nextflow.config b/nextflow.config index f3d63368..13e3824e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -123,7 +123,7 @@ process { mode: params.publish_mode, ] } - withName: 'count_aln_vcf' { + withName: 'compile_aln_vcf' { cpus = 1 disk = '50 GB' From 217ac20b3bbeb5aabdba61be8978f34dfa1f715d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:21:03 +0000 Subject: [PATCH 087/132] drop multiqc_plugins folder --- .../multiqc_npm/__init__.py | 0 bin/multiqc_plugins_BAK/multiqc_npm/cli.py | 16 ---- bin/multiqc_plugins_BAK/multiqc_npm/hooks.py | 26 ------- .../multiqc_npm/modules/__init__.py | 2 - .../modules/npm_calculate_callability.py | 34 --------- .../multiqc_npm/modules/npm_count_variants.py | 34 --------- .../multiqc_npm/modules/npm_extra_metrics.py | 55 -------------- .../modules/npm_picard_QualityYieldMetrics.py | 63 ---------------- .../modules/npm_samtools_stats_bq.py | 74 ------------------- bin/multiqc_plugins_BAK/setup.py | 39 ---------- 10 files changed, 343 deletions(-) delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/__init__.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/cli.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/hooks.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py delete mode 100644 bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py delete mode 100644 bin/multiqc_plugins_BAK/setup.py diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/__init__.py b/bin/multiqc_plugins_BAK/multiqc_npm/__init__.py deleted file mode 100644 index e69de29b..00000000 diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/cli.py b/bin/multiqc_plugins_BAK/multiqc_npm/cli.py deleted file mode 100644 index 2c1454f5..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/cli.py +++ /dev/null @@ -1,16 +0,0 @@ -#!/usr/bin/env python3 - -""" -Command line options - -See https://multiqc.info/docs/#command-line-options -""" - -import click - -enable_plugin = click.option('--enable-npm-plugin', - 'enable_plugin', - is_flag=True, - default=False, - help="Enable the MultiQC NPM plugin on this run" - ) diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/hooks.py b/bin/multiqc_plugins_BAK/multiqc_npm/hooks.py deleted file mode 100644 index d47965d7..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/hooks.py +++ /dev/null @@ -1,26 +0,0 @@ -#!/usr/bin/env python3 - -""" -Plugin hooks - -See https://multiqc.info/docs/#hooks -""" - -from __future__ import print_function -import logging -from pkg_resources import get_distribution - -from multiqc.utils import config - - -log = logging.getLogger('multiqc') - - -def before_config(): - my_search_patterns = { - 'multiqc_npm/picard_quality_yield_metrics': {'fn': '*.quality_yield_metrics.txt', 'shared': 'true'}, - 'multiqc_npm/samtools_stats_bq': {'fn': '*.stats', 'contents': 'This file was produced by samtools stats', 'shared': 'true'}, - 'multiqc_npm/count_variants': {'fn': '*.variant_counts.json'} - } - config.update_dict(config.sp, my_search_patterns) - log.info("Expanded search patterns with the following: %s", ", ".join(my_search_patterns.keys())) diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py deleted file mode 100644 index 79df8b47..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/__init__.py +++ /dev/null @@ -1,2 +0,0 @@ -from __future__ import absolute_import -from .npm_extra_metrics import NPMExtraMetrics diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py deleted file mode 100644 index f71b5347..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_calculate_callability.py +++ /dev/null @@ -1,34 +0,0 @@ -#!/usr/bin/env python3 - -""" -Parser for calculate_callability.py -""" - -import logging -import json - -from multiqc.utils import report - -log = logging.getLogger(__name__) - - -def parse_reports(self): - - # Set up vars - self.calculate_callability = dict() - - # Collect metrics - for f in self.find_log_files('multiqc_npm/calculate_callability'): - parsed_data = json.loads(f["f"]) - # Save results - s_name = f["s_name"] - self.calculate_callability[s_name] = parsed_data - - # Write results - if len(self.calculate_callability) > 0: - - # Write parsed data to a file - self.write_data_file(self.calculate_callability, 'multiqc_npm_calculate_callability') - - # Return the number of detected samples to the parent module - return len(self.calculate_callability) diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py deleted file mode 100644 index 49ccf96f..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_count_variants.py +++ /dev/null @@ -1,34 +0,0 @@ -#!/usr/bin/env python3 - -""" -Parser for count_variants.py -""" - -import logging -import json - -from multiqc.utils import report - -log = logging.getLogger(__name__) - - -def parse_reports(self): - - # Set up vars - self.count_variants = dict() - - # Collect metrics - for f in self.find_log_files('multiqc_npm/count_variants'): - parsed_data = json.loads(f["f"]) - # Save results - s_name = f["s_name"] - self.count_variants[s_name] = parsed_data - - # Write results - if len(self.count_variants) > 0: - - # Write parsed data to a file - self.write_data_file(self.count_variants, 'multiqc_npm_count_variants') - - # Return the number of detected samples to the parent module - return len(self.count_variants) diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py deleted file mode 100644 index a6516cf7..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_extra_metrics.py +++ /dev/null @@ -1,55 +0,0 @@ -#!/usr/bin/env python3 - -""" -NPMExtraMetrics module -""" - -from __future__ import print_function -import logging - -from multiqc.utils import config -from multiqc.modules.base_module import BaseMultiqcModule - -from . import npm_picard_QualityYieldMetrics -from . import npm_samtools_stats_bq -from . import npm_count_variants - -log = logging.getLogger('multiqc') - - -class NPMExtraMetrics(BaseMultiqcModule): - - def __init__(self): - - # Halt execution if we've disabled the plugin - if config.kwargs.get('enable_plugin', False) is True \ - or getattr(config, 'enable_plugin', False) is True: - log.info("Running with MultiQC NPM plugin") - else: - log.info("Skipping MultiQC NPM plugin as not enabled") - return None - - # Initialise the parent module Class object - super(NPMExtraMetrics, self).__init__( - name='NPMExtraMetrics', - anchor='npm_extra_metrics' - ) - - # Call each submodule and report progress - n = dict() - - n['npm_picard_QualityYieldMetrics'] = npm_picard_QualityYieldMetrics.parse_reports(self) - if n['npm_picard_QualityYieldMetrics'] > 0: - log.info("Found %d npm_picard_QualityYieldMetrics reports" % n['npm_picard_QualityYieldMetrics']) - - n['npm_samtools_stats_bq'] = npm_samtools_stats_bq.parse_reports(self) - if n['npm_samtools_stats_bq'] > 0: - log.info("Found %d npm_samtools_stats_bq reports" % n['npm_samtools_stats_bq']) - - n['npm_count_variants'] = npm_count_variants.parse_reports(self) - if n['npm_count_variants'] > 0: - log.info("Found %d npm_count_variants reports" % n['npm_count_variants']) - - # Exit if we didn't find anything - if sum(n.values()) == 0: - raise UserWarning diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py deleted file mode 100644 index 634c018a..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_picard_QualityYieldMetrics.py +++ /dev/null @@ -1,63 +0,0 @@ -#!/usr/bin/env python3 - -""" -Parser for picard QualityYieldMetrics -""" - -import logging -import os -import re - -from multiqc.utils import config, report - -log = logging.getLogger(__name__) - - -def parse_reports(self): - - # Set up vars - self.picard_quality_yield_metrics = dict() - - # Collect metrics - for f in self.find_log_files('multiqc_npm/picard_quality_yield_metrics', filehandles=True): - parsed_data = dict() - s_name = None - keys = None - - for l in f['f']: - - # Pull sample name from input - if 'QualityYieldMetrics' in l and 'INPUT' in l: - fn_search = re.search(r"INPUT(?:=|\s+)(\[?[^\s]+\]?)", l, flags=re.IGNORECASE) - if fn_search: - s_name = os.path.basename(fn_search.group(1).strip('[]')) - s_name = self.clean_s_name(s_name, f['root']) - # Remove file extension - s_name = os.path.splitext(s_name)[0] - print(s_name, "clean file name without extension") - - - # Parse metric names and values - if s_name is not None: - if 'QualityYieldMetrics' in l and '## METRICS CLASS' in l: - keys = f['f'].readline().strip("\n").split("\t") - elif keys: - vals = l.strip("\n").split("\t") - if len(vals) == len(keys): - for i, k in enumerate(keys): - try: - parsed_data[k] = float(vals[i]) - except ValueError: - parsed_data[k] = vals[i] - - # Save results - self.picard_quality_yield_metrics[s_name] = parsed_data - - # Write results - if len(self.picard_quality_yield_metrics) > 0: - - # Write parsed data to a file - self.write_data_file(self.picard_quality_yield_metrics, 'multiqc_npm_picard_QualityYieldMetrics') - - # Return the number of detected samples to the parent module - return len(self.picard_quality_yield_metrics) diff --git a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py b/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py deleted file mode 100644 index 2d77a797..00000000 --- a/bin/multiqc_plugins_BAK/multiqc_npm/modules/npm_samtools_stats_bq.py +++ /dev/null @@ -1,74 +0,0 @@ -#!/usr/bin/env python3 - -""" -Parser for samtools stats -""" - -import logging -import pandas as pd -import numpy as np - -from multiqc.utils import report - -log = logging.getLogger(__name__) - - -def calc_pct_q30_bases(x): - df = pd.DataFrame(x).apply(pd.to_numeric) - df.columns = ["cycle_number"] + ["bq_%d" % i for i in range(0, len(df.columns)-1, 1)] - - bq_all = df.loc[:, 'bq_0':] - bq_ge30 = df.loc[:, 'bq_30':] - - sum_bases_q30 = int(bq_ge30.values.sum()) - sum_bases_all = int(bq_all.values.sum()) - pct_q30_bases = np.round(np.divide(sum_bases_q30, sum_bases_all)*100, 2) - - return pct_q30_bases, sum_bases_q30, sum_bases_all - - -def parse_reports(self): - - # Set up vars - self.samtools_stats_bq = dict() - - # Collect metrics - for f in self.find_log_files('multiqc_npm/samtools_stats_bq', filehandles=True): - - # Collect relevant records and calculate metrics - parsed_data = dict() - ffq = list() - lfq = list() - - for l in f['f']: - if l.startswith("FFQ"): - vals = l.strip("\n").split("\t") - ffq.append(vals[1:]) - elif l.startswith("LFQ"): - vals = l.strip("\n").split("\t") - lfq.append(vals[1:]) - else: - continue - - pct, total, q30 = calc_pct_q30_bases(ffq) - parsed_data["pct_q30_bases_read1"] = pct - parsed_data["total_bases_read1"] = total - parsed_data["q30_bases_read1"] = q30 - - pct, total, q30 = calc_pct_q30_bases(lfq) - parsed_data["pct_q30_bases_read2"] = pct - parsed_data["total_bases_read2"] = total - parsed_data["q30_bases_read2"] = q30 - - # Save results - s_name = f["s_name"] - self.samtools_stats_bq[s_name] = parsed_data - - # Write results - if len(self.samtools_stats_bq) > 0: - - # Write parsed data to a file - self.write_data_file(self.samtools_stats_bq, 'multiqc_npm_samtools_stats_bq') - - # Return the number of detected samples to the parent module - return len(self.samtools_stats_bq) diff --git a/bin/multiqc_plugins_BAK/setup.py b/bin/multiqc_plugins_BAK/setup.py deleted file mode 100644 index fb0b791b..00000000 --- a/bin/multiqc_plugins_BAK/setup.py +++ /dev/null @@ -1,39 +0,0 @@ -#!/usr/bin/env python3 - -""" -A MultiQC plugin for downstream analysis of NPM data -""" - -from setuptools import setup, find_packages - -version = '0.1' - -setup( - name='multiqc_npm', - version=version, - author='Mar Gonzalez-Porta', - author_email='Mar_Gonzalez_Porta@gis.a-star.edu.sg', - description="MultiQC plugin for NPM", - long_description=__doc__, - keywords='bioinformatics', - url='https://github.com/c-BIG/NPM-sample-qc', - download_url='https://github.com/c-BIG/NPM-sample-qc/releases', - license='MIT', - packages=find_packages(), - include_package_data=True, - install_requires=[ - 'multiqc', - 'pandas' - ], - entry_points={ - 'multiqc.modules.v1': [ - 'multiqc_npm = multiqc_npm.modules.npm_extra_metrics:NPMExtraMetrics' - ], - 'multiqc.cli_options.v1': [ - 'enable-npm-plugin = multiqc_npm.cli:enable_plugin' - ], - 'multiqc.hooks.v1': [ - 'before_config = multiqc_npm.hooks:before_config' - ] - } -) From 3dc1bf0a1c62951c538760d2da26c6ef6446c64d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:40:38 +0000 Subject: [PATCH 088/132] drop multiqc config --- nextflow.config | 11 ----------- 1 file changed, 11 deletions(-) diff --git a/nextflow.config b/nextflow.config index 13e3824e..555b2c28 100644 --- a/nextflow.config +++ b/nextflow.config @@ -134,17 +134,6 @@ process { mode: params.publish_mode, ] } - withName: 'multiqc' { - - cpus = 1 - disk = '50 GB' - memory = { 2.GB * task.attempt } - - publishDir = [ - path: "${params.publishdir}/multiqc", - mode: params.publish_mode, - ] - } } plugins { From f2aaf3e6f8b22ac81385d04029b3d87e8c23c595 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 08:43:38 +0000 Subject: [PATCH 089/132] variable name update on count_variants.py --- bin/count_variants.py | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/bin/count_variants.py b/bin/count_variants.py index ca74af3c..bc4cd6fc 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -166,9 +166,11 @@ def count_variants(input_vcf, scratch_dir, regions): # exclude multi-allelic sites cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" # if length(ALT) > length(REF), label as INS - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + #cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" + cmd += " | awk '{ if( length($5) > length($4) ) { print \"insertions\" }" # if length(ALT) < length(REF), label as DEL - cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + #cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" + cmd += " else if ( length($5) < length($4) ) { print \"deletions\" }" # label everything else as UNK and report its location cmd += " else { print \"UNK\" } }'" # count by label @@ -178,7 +180,7 @@ def count_variants(input_vcf, scratch_dir, regions): x = p.stdout.read() for l in x.splitlines(): l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() + k = "count_%s" % l[1].lower() v = int(l[0]) r[k] = v From daf64bd867b8ed3f525f025862d9bd9629ab653d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 09:09:36 +0000 Subject: [PATCH 090/132] results of aln, varaints and compiled metrics json --- .../.samtools.metrics | 2 -- .../NA12878-chr14-AKT1.aln.metrics.json | 15 +++++++++++++++ .../metrics/NA12878-chr14-AKT1.metrics.json | 19 +++++++++---------- .../NA12878-chr14-AKT1.variant.metrics.json | 14 ++++++++++++++ .../params.yaml.BAK | 5 ----- 5 files changed, 38 insertions(+), 17 deletions(-) delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics deleted file mode 100644 index 57cdadea..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/.samtools.metrics +++ /dev/null @@ -1,2 +0,0 @@ -99.6307 -99.6307 diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json new file mode 100644 index 00000000..9d09d556 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json @@ -0,0 +1,15 @@ +{ + "biosample": { + "id": "NA12878-chr14-AKT1" + }, + "wgs_qc_metrics": { + "insert_size_std_deviation": "98.2", + "mad_autosome_coverage": "0", + "mean_autosome_coverage": "0.000644", + "mean_insert_size": "447.3", + "pct_autosomes_15x": "0.000016", + "pct_reads_mapped": "99.63", + "pct_reads_properly_paired": "97.9", + "yield_bp_q30": "1996315" + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json index 8a33e1a2..ee117097 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json @@ -6,18 +6,17 @@ "count_deletions": 848, "count_insertions": 850, "count_snvs": 8489, - "cross_contamination_rate": "NA", - "insert_size_std_deviation": 98.2, - "mad_autosome_coverage": 0.0, - "mean_autosome_coverage": 0.00064, - "mean_insert_size": 447.3, - "pct_autosomes_15x": 0.0016, - "pct_reads_mapped": 99.6307, - "pct_reads_properly_paired": 97.87835, + "insert_size_std_deviation": "98.2", + "mad_autosome_coverage": "0", + "mean_autosome_coverage": "0.000644", + "mean_insert_size": "447.3", + "pct_autosomes_15x": "0.000016", + "pct_reads_mapped": "99.63", + "pct_reads_properly_paired": "97.9", "ratio_heterozygous_homzygous_indel": 1.49, "ratio_heterozygous_homzygous_snv": 1.05, "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions": 2.13, - "yield_bp_q30": 1996315 + "ratio_transitions_transversions_snv": 2.13, + "yield_bp_q30": "1996315" } } diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json new file mode 100644 index 00000000..899b9a34 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json @@ -0,0 +1,14 @@ +{ + "biosample": { + "id": "NA12878-chr14-AKT1" + }, + "wgs_qc_metrics": { + "count_deletions": 848, + "count_insertions": 850, + "count_snvs": 8489, + "ratio_heterozygous_homzygous_indel": 1.49, + "ratio_heterozygous_homzygous_snv": 1.05, + "ratio_insertion_deletion": 1.0, + "ratio_transitions_transversions_snv": 2.13 + } +} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK deleted file mode 100644 index 5e6ea776..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.BAK +++ /dev/null @@ -1,5 +0,0 @@ -samples: -- - biosample_id: NA12878-chr14-AKT1 - aln: NA12878-chr14-AKT1.bam - vcf: NA12878-chr14-AKT1.vcf.gz From 695937797df0acd978b5b698ac0c82068c47fe26 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 3 Sep 2024 09:11:05 +0000 Subject: [PATCH 091/132] dropped ouput backup folder --- .../output_bak/pipeline_info/dag.pdf | Bin 18211 -> 0 bytes .../output_bak/pipeline_info/report.html | 1044 -- .../output_bak/pipeline_info/timeline.html | 224 - .../output_bak/pipeline_info/trace.txt | 4 - .../bcftools/NA12878-chr14-AKT1.pass.stats | 1805 --- .../NA12878-chr14-AKT1.variant_counts.json | 16 - .../multiqc/NA12878-chr14-AKT1.metrics.json | 23 - .../multiqc_data/multiqc.log | 28 - 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        Nextflow workflow report

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        [silly_lalande]

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        Run times
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        - 29-May-2024 09:35:31 - 29-May-2024 09:35:45 - (duration: 13.8s) -
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          3 succeeded  
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        Nextflow command
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        nextflow run ../../main.nf -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config --inputs_list params.yaml -work-dir ./work --publish_dir ./output
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        /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6
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        /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work
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        version 23.09.1-edge, build 5881 (11-09-2023 10:08 UTC)
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        Resource Usage

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        Tasks

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        This table shows information about each task in the workflow. Use the search box on the right - to filter rows for specific values. Clicking headers will sort the table by that value and - scrolling side to side will reveal more columns.

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        - Generated by Nextflow, version 23.09.1-edge -
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        - - - - - diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html deleted file mode 100644 index cf0816c6..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/timeline.html +++ /dev/null @@ -1,224 +0,0 @@ - - - - - - - - - - - - -
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        Processes execution timeline

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        - Launch time:
        - Elapsed time:
        - Legend: job wall time / memory usage (RAM) -

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        - - - - - - - diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt deleted file mode 100644 index a56a7fb8..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/pipeline_info/trace.txt +++ /dev/null @@ -1,4 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar -2 b6/e2ba69 885662 bcftools_stats (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:34.815 3.3s 53ms 1178.9% 3.4 MB 4.7 MB 678.8 KB 38.8 KB -1 d7/181748 885992 count_variants (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:38.138 2.1s 1.4s 1108.0% 49.8 MB 698.4 MB 22.9 MB 10.5 MB -3 d4/01e073 886629 multiqc (NA12878-chr14-AKT1) COMPLETED 0 2024-05-29 09:35:40.222 5s 4.3s 148.3% 117.6 MB 866.8 MB 49.6 MB 17.7 MB diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats deleted file mode 100644 index 6b47284e..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/bcftools/NA12878-chr14-AKT1.pass.stats +++ /dev/null @@ -1,1805 +0,0 @@ -# This file was produced by bcftools stats (1.15.1+htslib-1.19) and can be plotted using plot-vcfstats. -# The command line was: bcftools stats -f PASS NA12878-chr14-AKT1.vcf.gz -# -# Definition of sets: -# ID [2]id [3]tab-separated file names -ID 0 NA12878-chr14-AKT1.vcf.gz -# SN, Summary numbers: -# number of records .. number of data rows in the VCF -# number of no-ALTs .. reference-only sites, ALT is either "." or identical to REF -# number of SNPs .. number of rows with a SNP -# number of MNPs .. number of rows with a MNP, such as CC>TT -# number of indels .. number of rows with an indel -# number of others .. number of rows with other type, for example a symbolic allele or -# a complex substitution, such as ACT>TCGA -# number of multiallelic sites .. number of rows with multiple alternate alleles -# number of multiallelic SNP sites .. number of rows with multiple alternate alleles, all SNPs -# -# Note that rows containing multiple types will be counted multiple times, in each -# counter. For example, a row with a SNP and an indel increments both the SNP and -# the indel counter. -# -# SN [2]id [3]key [4]value -SN 0 number of samples: 1 -SN 0 number of records: 10321 -SN 0 number of no-ALTs: 0 -SN 0 number of SNPs: 8489 -SN 0 number of MNPs: 0 -SN 0 number of indels: 1843 -SN 0 number of others: 0 -SN 0 number of multiallelic sites: 151 -SN 0 number of multiallelic SNP sites: 6 -# TSTV, transitions/transversions: -# TSTV [2]id [3]ts [4]tv [5]ts/tv [6]ts (1st ALT) [7]tv (1st ALT) [8]ts/tv (1st ALT) -TSTV 0 5784 2711 2.13 5776 2702 2.14 -# SiS, Singleton stats: -# SiS [2]id [3]allele count [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable -SiS 0 1 4347 2983 1364 1237 0 0 1237 -# AF, Stats by non-reference allele frequency: -# AF [2]id [3]allele frequency [4]number of SNPs [5]number of transitions [6]number of transversions [7]number of indels [8]repeat-consistent [9]repeat-inconsistent [10]not applicable -AF 0 0.000000 4347 2983 1364 1237 0 0 1237 -AF 0 0.990000 4148 2801 1347 740 0 0 740 -# QUAL, Stats by quality -# QUAL [2]id [3]Quality [4]number of SNPs [5]number of transitions (1st ALT) [6]number of transversions (1st ALT) [7]number of indels -QUAL 0 5.0 3 1 2 0 -QUAL 0 5.2 2 2 0 0 -QUAL 0 5.4 1 0 1 1 -QUAL 0 5.5 3 1 2 0 -QUAL 0 5.9 0 0 0 4 -QUAL 0 6.2 0 0 0 1 -QUAL 0 6.3 0 0 0 1 -QUAL 0 6.4 2 1 1 1 -QUAL 0 6.5 2 2 0 1 -QUAL 0 7.0 1 1 0 1 -QUAL 0 7.1 1 1 0 1 -QUAL 0 7.2 1 1 0 0 -QUAL 0 7.3 1 1 0 0 -QUAL 0 7.4 2 1 1 0 -QUAL 0 7.5 1 1 0 0 -QUAL 0 7.6 15 7 8 5 -QUAL 0 7.7 1 0 1 0 -QUAL 0 7.8 3 2 1 1 -QUAL 0 7.9 1 1 0 0 -QUAL 0 8.1 2 1 1 0 -QUAL 0 8.4 1 1 0 0 -QUAL 0 8.5 1 1 0 0 -QUAL 0 8.6 4 2 2 1 -QUAL 0 8.7 1 1 0 2 -QUAL 0 8.9 4 4 0 0 -QUAL 0 9.0 1 1 0 0 -QUAL 0 9.1 3 2 1 1 -QUAL 0 9.3 4 3 1 0 -QUAL 0 9.4 7 6 1 1 -QUAL 0 9.5 12 6 6 1 -QUAL 0 9.7 1 1 0 0 -QUAL 0 9.8 1 0 1 0 -QUAL 0 9.9 2 1 1 0 -QUAL 0 10.2 1 0 1 0 -QUAL 0 10.3 1 1 0 0 -QUAL 0 10.4 0 0 0 1 -QUAL 0 10.5 1 0 1 0 -QUAL 0 10.6 1 1 0 0 -QUAL 0 10.7 1 1 0 1 -QUAL 0 11.0 1 1 0 0 -QUAL 0 11.1 1 1 0 0 -QUAL 0 11.3 4 2 2 0 -QUAL 0 11.4 4 2 2 1 -QUAL 0 11.5 0 0 0 2 -QUAL 0 11.6 1 0 1 0 -QUAL 0 11.7 1 1 0 0 -QUAL 0 11.8 6 3 3 0 -QUAL 0 11.9 2 2 0 0 -QUAL 0 12.0 1 0 1 0 -QUAL 0 12.1 5 5 0 0 -QUAL 0 12.2 1 1 0 0 -QUAL 0 12.3 1 0 1 0 -QUAL 0 12.4 3 3 0 1 -QUAL 0 12.5 1 1 0 0 -QUAL 0 12.6 2 2 0 1 -QUAL 0 12.7 3 2 1 0 -QUAL 0 12.8 2 1 1 0 -QUAL 0 12.9 1 1 0 0 -QUAL 0 13.0 3 0 3 0 -QUAL 0 13.1 1 1 0 0 -QUAL 0 13.3 0 0 0 1 -QUAL 0 13.5 1 1 0 0 -QUAL 0 13.6 0 0 0 1 -QUAL 0 13.7 3 1 2 0 -QUAL 0 13.8 6 2 4 1 -QUAL 0 14.1 10 6 4 1 -QUAL 0 14.2 4 3 1 0 -QUAL 0 14.3 7 3 4 0 -QUAL 0 14.4 4 2 2 0 -QUAL 0 14.5 0 0 0 1 -QUAL 0 14.6 1 1 0 4 -QUAL 0 14.7 1 1 0 0 -QUAL 0 14.8 2 1 1 0 -QUAL 0 14.9 3 2 1 1 -QUAL 0 15.1 2 0 2 0 -QUAL 0 15.2 1 1 0 0 -QUAL 0 15.3 0 0 0 1 -QUAL 0 15.7 1 0 1 0 -QUAL 0 15.8 4 2 2 0 -QUAL 0 15.9 4 1 3 0 -QUAL 0 16.0 1 1 0 0 -QUAL 0 16.1 1 0 1 0 -QUAL 0 16.2 2 1 1 0 -QUAL 0 16.3 2 1 1 0 -QUAL 0 16.5 0 0 0 1 -QUAL 0 16.6 5 2 3 0 -QUAL 0 16.8 4 2 2 0 -QUAL 0 17.0 2 2 0 0 -QUAL 0 17.1 3 3 0 0 -QUAL 0 17.2 1 1 0 0 -QUAL 0 17.3 2 2 0 0 -QUAL 0 17.6 0 0 0 1 -QUAL 0 17.7 1 1 0 1 -QUAL 0 17.8 1 1 0 1 -QUAL 0 18.1 4 2 2 0 -QUAL 0 18.2 2 1 1 0 -QUAL 0 18.3 1 1 0 0 -QUAL 0 18.4 2 1 1 0 -QUAL 0 18.5 1 0 1 0 -QUAL 0 18.6 6 3 3 0 -QUAL 0 18.8 9 5 4 0 -QUAL 0 18.9 4 2 2 1 -QUAL 0 19.0 4 4 0 0 -QUAL 0 19.1 12 11 1 1 -QUAL 0 19.3 3 1 2 0 -QUAL 0 19.4 1 0 1 0 -QUAL 0 19.5 1 0 1 0 -QUAL 0 19.7 2 2 0 0 -QUAL 0 19.8 0 0 0 1 -QUAL 0 19.9 6 4 2 0 -QUAL 0 20.0 2 1 1 0 -QUAL 0 20.1 1 1 0 1 -QUAL 0 20.2 3 3 0 0 -QUAL 0 20.4 1 1 0 0 -QUAL 0 20.5 2 2 0 0 -QUAL 0 20.6 1 0 1 2 -QUAL 0 20.7 1 1 0 1 -QUAL 0 20.8 2 1 1 0 -QUAL 0 21.0 7 7 0 1 -QUAL 0 21.1 3 1 2 1 -QUAL 0 21.2 0 0 0 1 -QUAL 0 21.3 5 2 3 0 -QUAL 0 21.4 1 1 0 0 -QUAL 0 21.5 2 1 1 0 -QUAL 0 21.6 2 1 1 0 -QUAL 0 21.8 3 2 1 0 -QUAL 0 22.0 1 1 0 0 -QUAL 0 22.1 2 1 1 0 -QUAL 0 22.2 2 2 0 0 -QUAL 0 22.3 1 0 1 0 -QUAL 0 22.6 0 0 0 1 -QUAL 0 22.7 2 1 1 0 -QUAL 0 22.8 4 3 1 0 -QUAL 0 22.9 4 2 2 0 -QUAL 0 23.1 1 0 1 0 -QUAL 0 23.2 1 0 1 1 -QUAL 0 23.3 2 1 1 0 -QUAL 0 23.5 5 3 2 0 -QUAL 0 23.6 1 1 0 0 -QUAL 0 23.7 2 2 0 0 -QUAL 0 23.9 1 0 1 0 -QUAL 0 24.2 0 0 0 1 -QUAL 0 24.3 2 0 2 0 -QUAL 0 24.4 1 1 0 0 -QUAL 0 24.5 1 1 0 0 -QUAL 0 24.6 4 1 3 1 -QUAL 0 24.7 5 3 2 0 -QUAL 0 24.8 1 0 1 0 -QUAL 0 24.9 1 0 1 0 -QUAL 0 25.0 9 5 4 0 -QUAL 0 25.2 3 2 1 0 -QUAL 0 25.3 9 5 4 2 -QUAL 0 25.6 0 0 0 1 -QUAL 0 25.7 12 7 5 0 -QUAL 0 25.8 3 2 1 0 -QUAL 0 26.0 2 1 1 0 -QUAL 0 26.1 1 1 0 0 -QUAL 0 26.2 1 1 0 0 -QUAL 0 26.3 9 6 3 3 -QUAL 0 26.5 6 3 3 1 -QUAL 0 26.6 0 0 0 1 -QUAL 0 26.7 4 4 0 0 -QUAL 0 26.8 1 0 1 0 -QUAL 0 27.0 9 4 5 0 -QUAL 0 27.2 1 1 0 0 -QUAL 0 27.3 3 1 2 1 -QUAL 0 27.4 1 1 0 0 -QUAL 0 27.5 4 2 2 0 -QUAL 0 27.7 3 3 0 0 -QUAL 0 27.8 1 1 0 1 -QUAL 0 27.9 1 0 1 1 -QUAL 0 28.0 1 1 0 0 -QUAL 0 28.1 1 1 0 1 -QUAL 0 28.2 1 1 0 0 -QUAL 0 28.3 4 2 2 0 -QUAL 0 28.4 1 1 0 0 -QUAL 0 28.5 1 0 1 0 -QUAL 0 28.6 2 1 1 0 -QUAL 0 28.7 1 1 0 1 -QUAL 0 28.9 4 1 3 1 -QUAL 0 29.0 5 4 1 0 -QUAL 0 29.1 1 0 1 0 -QUAL 0 29.2 4 3 1 1 -QUAL 0 29.3 2 1 1 2 -QUAL 0 29.4 4 3 1 1 -QUAL 0 29.5 0 0 0 1 -QUAL 0 29.6 2 2 0 0 -QUAL 0 29.7 1 1 0 1 -QUAL 0 29.8 1 1 0 0 -QUAL 0 29.9 0 0 0 1 -QUAL 0 30.0 8 3 5 0 -QUAL 0 30.1 1 1 0 0 -QUAL 0 30.2 4 2 2 0 -QUAL 0 30.4 3 2 1 0 -QUAL 0 30.5 8 8 0 1 -QUAL 0 30.6 2 1 1 0 -QUAL 0 30.7 5 4 1 0 -QUAL 0 30.8 3 1 2 0 -QUAL 0 31.0 1 1 0 0 -QUAL 0 31.1 2 2 0 0 -QUAL 0 31.2 2 2 0 0 -QUAL 0 31.3 3 2 1 1 -QUAL 0 31.6 2 1 1 0 -QUAL 0 31.7 0 0 0 1 -QUAL 0 31.8 0 0 0 1 -QUAL 0 32.0 2 2 0 1 -QUAL 0 32.1 2 2 0 0 -QUAL 0 32.2 2 0 2 0 -QUAL 0 32.3 3 1 2 0 -QUAL 0 32.4 2 1 1 1 -QUAL 0 32.5 6 3 3 3 -QUAL 0 32.6 0 0 0 1 -QUAL 0 32.7 1 0 1 0 -QUAL 0 32.8 7 7 0 2 -QUAL 0 33.0 8 3 5 4 -QUAL 0 33.2 1 0 1 0 -QUAL 0 33.3 5 4 1 1 -QUAL 0 33.4 2 1 1 0 -QUAL 0 33.5 35 23 12 0 -QUAL 0 33.6 7 2 5 0 -QUAL 0 33.7 2 2 0 1 -QUAL 0 33.8 2 2 0 1 -QUAL 0 33.9 2 1 1 0 -QUAL 0 34.0 2 2 0 0 -QUAL 0 34.1 0 0 0 1 -QUAL 0 34.2 4 4 0 0 -QUAL 0 34.3 9 3 6 0 -QUAL 0 34.4 1 1 0 1 -QUAL 0 34.5 1 1 0 0 -QUAL 0 34.6 1 0 1 0 -QUAL 0 34.7 3 2 1 1 -QUAL 0 34.8 3 0 3 0 -QUAL 0 34.9 1 1 0 0 -QUAL 0 35.0 4 4 0 1 -QUAL 0 35.1 3 3 0 2 -QUAL 0 35.2 3 2 1 1 -QUAL 0 35.3 3 3 0 1 -QUAL 0 35.4 3 1 2 0 -QUAL 0 35.5 3 3 0 0 -QUAL 0 35.7 6 3 3 0 -QUAL 0 35.8 3 3 0 0 -QUAL 0 36.0 6 2 4 0 -QUAL 0 36.2 1 1 0 2 -QUAL 0 36.3 1 1 0 2 -QUAL 0 36.5 6 4 2 0 -QUAL 0 36.6 5 2 3 2 -QUAL 0 36.7 1 1 0 2 -QUAL 0 36.8 1 0 1 1 -QUAL 0 36.9 2 1 1 0 -QUAL 0 37.1 3 2 1 2 -QUAL 0 37.2 4 3 1 0 -QUAL 0 37.3 1 1 0 2 -QUAL 0 37.4 1 0 1 1 -QUAL 0 37.5 4 3 1 0 -QUAL 0 37.7 5 3 2 2 -QUAL 0 37.8 1 0 1 1 -QUAL 0 37.9 4 3 1 0 -QUAL 0 38.0 3 1 2 0 -QUAL 0 38.1 4 4 0 1 -QUAL 0 38.2 1 1 0 0 -QUAL 0 38.3 4 3 1 1 -QUAL 0 38.4 1 0 1 0 -QUAL 0 38.5 5 5 0 1 -QUAL 0 38.6 4 1 3 0 -QUAL 0 38.7 2 1 1 1 -QUAL 0 38.8 2 0 2 1 -QUAL 0 38.9 2 1 1 0 -QUAL 0 39.0 2 2 0 2 -QUAL 0 39.1 2 1 1 1 -QUAL 0 39.2 3 2 1 1 -QUAL 0 39.3 15 11 4 0 -QUAL 0 39.4 5 4 1 2 -QUAL 0 39.5 1 1 0 1 -QUAL 0 39.6 4 1 3 1 -QUAL 0 39.7 4 2 2 0 -QUAL 0 39.8 1 1 0 0 -QUAL 0 39.9 7 5 2 2 -QUAL 0 40.0 2 1 1 1 -QUAL 0 40.1 7 5 2 3 -QUAL 0 40.2 5 2 3 0 -QUAL 0 40.3 5 2 3 1 -QUAL 0 40.4 2 1 1 0 -QUAL 0 40.5 3 3 0 2 -QUAL 0 40.6 7 4 3 1 -QUAL 0 40.7 4 2 2 1 -QUAL 0 40.8 9 8 1 0 -QUAL 0 40.9 4 4 0 1 -QUAL 0 41.0 13 11 2 0 -QUAL 0 41.1 25 21 4 1 -QUAL 0 41.2 3 2 1 1 -QUAL 0 41.3 6 4 2 0 -QUAL 0 41.4 3 2 1 0 -QUAL 0 41.5 8 5 3 2 -QUAL 0 41.6 5 3 2 0 -QUAL 0 41.7 4 3 1 1 -QUAL 0 41.8 9 5 4 0 -QUAL 0 41.9 1 0 1 0 -QUAL 0 42.0 7 4 3 1 -QUAL 0 42.1 6 5 1 0 -QUAL 0 42.2 6 4 2 1 -QUAL 0 42.3 7 6 1 1 -QUAL 0 42.4 2 0 2 0 -QUAL 0 42.5 14 8 6 1 -QUAL 0 42.6 12 7 5 2 -QUAL 0 42.7 3 2 1 0 -QUAL 0 42.8 7 4 3 2 -QUAL 0 42.9 6 4 2 1 -QUAL 0 43.0 14 10 4 1 -QUAL 0 43.1 9 7 2 1 -QUAL 0 43.2 0 0 0 3 -QUAL 0 43.3 15 12 3 2 -QUAL 0 43.4 4 3 1 1 -QUAL 0 43.5 10 8 2 3 -QUAL 0 43.6 6 3 3 0 -QUAL 0 43.7 14 9 5 4 -QUAL 0 43.8 20 11 9 3 -QUAL 0 43.9 5 2 3 3 -QUAL 0 44.0 4 2 2 1 -QUAL 0 44.1 17 10 7 2 -QUAL 0 44.2 15 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28 23 5 7 -QUAL 0 48.7 31 24 7 5 -QUAL 0 48.8 33 25 8 10 -QUAL 0 48.9 44 32 12 13 -QUAL 0 49.0 38 28 10 15 -QUAL 0 49.1 30 18 12 14 -QUAL 0 49.2 41 31 10 12 -QUAL 0 49.3 46 33 13 13 -QUAL 0 49.4 59 41 18 16 -QUAL 0 49.5 58 46 12 7 -QUAL 0 49.6 89 62 27 15 -QUAL 0 49.7 129 100 29 14 -QUAL 0 49.8 55 43 12 18 -QUAL 0 49.9 208 149 59 46 -QUAL 0 50.0 1826 1264 562 414 -QUAL 0 50.1 3 3 0 4 -QUAL 0 50.2 1 1 0 1 -QUAL 0 50.3 5 5 0 0 -QUAL 0 50.4 3 2 1 1 -QUAL 0 50.5 0 0 0 1 -QUAL 0 50.6 0 0 0 1 -QUAL 0 50.7 3 2 1 1 -QUAL 0 50.9 1 1 0 3 -QUAL 0 51.0 2 2 0 0 -QUAL 0 51.2 0 0 0 1 -QUAL 0 51.3 0 0 0 1 -QUAL 0 51.4 2 1 1 0 -QUAL 0 51.6 5 4 1 0 -QUAL 0 51.7 1 1 0 1 -QUAL 0 51.9 1 1 0 0 -QUAL 0 52.0 2 2 0 0 -QUAL 0 52.1 1 1 0 0 -QUAL 0 52.2 4 2 2 0 -QUAL 0 52.5 2 1 1 0 -QUAL 0 52.6 1 0 1 0 -QUAL 0 52.7 1 1 0 0 -QUAL 0 52.8 1 1 0 0 -QUAL 0 53.0 4 3 1 1 -QUAL 0 53.1 4 3 1 0 -QUAL 0 53.4 1 0 1 1 -QUAL 0 53.6 0 0 0 1 -QUAL 0 53.7 2 2 0 0 -QUAL 0 53.8 2 2 0 0 -QUAL 0 54.0 1 0 1 0 -QUAL 0 54.4 0 0 0 2 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-QUAL 0 272.7 0 0 0 1 -QUAL 0 274.0 0 0 0 1 -QUAL 0 274.4 0 0 0 1 -QUAL 0 275.3 1 0 1 0 -QUAL 0 278.0 0 0 0 1 -QUAL 0 279.8 0 0 0 1 -QUAL 0 280.3 1 1 0 0 -QUAL 0 282.1 0 0 0 1 -QUAL 0 282.4 0 0 0 1 -QUAL 0 282.7 1 0 1 0 -QUAL 0 283.2 0 0 0 1 -QUAL 0 285.8 0 0 0 1 -QUAL 0 287.4 0 0 0 1 -QUAL 0 288.5 0 0 0 1 -QUAL 0 288.9 1 1 0 0 -QUAL 0 292.5 0 0 0 1 -QUAL 0 296.1 1 1 0 0 -QUAL 0 296.9 0 0 0 1 -QUAL 0 299.3 0 0 0 1 -QUAL 0 300.2 1 1 0 1 -QUAL 0 300.7 0 0 0 1 -QUAL 0 301.3 1 0 1 0 -QUAL 0 301.4 1 1 0 0 -QUAL 0 303.4 0 0 0 1 -QUAL 0 303.5 1 0 1 0 -QUAL 0 307.1 0 0 0 1 -QUAL 0 310.6 0 0 0 1 -QUAL 0 311.3 0 0 0 1 -QUAL 0 311.7 1 1 0 0 -QUAL 0 314.1 0 0 0 1 -QUAL 0 315.8 0 0 0 1 -QUAL 0 317.9 0 0 0 1 -QUAL 0 322.5 0 0 0 1 -QUAL 0 323.5 0 0 0 1 -QUAL 0 327.2 2 1 1 0 -QUAL 0 328.8 0 0 0 1 -QUAL 0 329.0 0 0 0 1 -QUAL 0 330.8 0 0 0 1 -QUAL 0 334.8 1 1 0 0 -QUAL 0 334.9 1 0 1 0 -QUAL 0 336.8 0 0 0 1 -QUAL 0 342.5 0 0 0 1 -QUAL 0 343.9 1 1 0 0 -QUAL 0 344.2 0 0 0 1 -QUAL 0 347.8 1 1 0 0 -QUAL 0 357.4 0 0 0 1 -QUAL 0 358.9 1 0 1 0 -QUAL 0 359.0 1 1 0 0 -QUAL 0 359.8 1 0 1 0 -QUAL 0 362.7 0 0 0 1 -QUAL 0 364.6 0 0 0 1 -QUAL 0 366.3 1 0 1 0 -QUAL 0 367.5 0 0 0 1 -QUAL 0 369.1 0 0 0 1 -QUAL 0 371.5 1 0 1 0 -QUAL 0 371.9 0 0 0 1 -QUAL 0 373.5 1 1 0 0 -QUAL 0 374.9 1 0 1 0 -QUAL 0 375.1 0 0 0 1 -QUAL 0 375.5 0 0 0 1 -QUAL 0 376.1 0 0 0 1 -QUAL 0 377.5 1 0 1 0 -QUAL 0 381.1 1 0 1 0 -QUAL 0 381.9 1 0 1 0 -QUAL 0 389.1 1 1 0 0 -QUAL 0 394.7 0 0 0 1 -QUAL 0 396.3 1 1 0 0 -QUAL 0 399.5 1 1 0 0 -QUAL 0 402.4 1 1 0 0 -QUAL 0 405.9 1 1 0 0 -QUAL 0 408.5 0 0 0 1 -QUAL 0 413.9 0 0 0 1 -QUAL 0 414.0 0 0 0 1 -QUAL 0 415.6 0 0 0 1 -QUAL 0 417.6 0 0 0 1 -QUAL 0 419.6 0 0 0 1 -QUAL 0 421.3 1 0 1 0 -QUAL 0 423.2 1 1 0 0 -QUAL 0 424.3 0 0 0 1 -QUAL 0 425.1 1 1 0 0 -QUAL 0 428.5 1 1 0 0 -QUAL 0 447.5 1 1 0 0 -QUAL 0 450.0 0 0 0 1 -QUAL 0 472.2 1 1 0 0 -QUAL 0 476.0 0 0 0 1 -QUAL 0 476.4 0 0 0 1 -QUAL 0 483.1 0 0 0 1 -QUAL 0 483.5 1 0 1 0 -QUAL 0 485.7 1 1 0 0 -QUAL 0 495.1 1 1 0 0 -QUAL 0 500.3 1 1 0 0 -QUAL 0 501.8 1 0 1 0 -QUAL 0 506.6 0 0 0 1 -QUAL 0 513.0 0 0 0 1 -QUAL 0 516.2 0 0 0 1 -QUAL 0 528.2 1 0 1 0 -QUAL 0 530.5 1 1 0 0 -QUAL 0 535.7 0 0 0 1 -QUAL 0 557.5 1 1 0 0 -QUAL 0 558.0 1 1 0 0 -QUAL 0 578.3 0 0 0 1 -QUAL 0 579.4 1 0 1 0 -QUAL 0 591.2 1 1 0 0 -QUAL 0 591.3 1 1 0 0 -QUAL 0 599.6 1 1 0 0 -QUAL 0 605.6 1 0 1 0 -QUAL 0 605.8 1 1 0 0 -QUAL 0 612.9 1 1 0 0 -QUAL 0 615.9 1 1 0 0 -QUAL 0 627.2 1 0 1 0 -QUAL 0 629.8 1 1 0 0 -QUAL 0 631.0 1 1 0 0 -QUAL 0 648.8 1 0 1 0 -QUAL 0 650.9 1 1 0 0 -QUAL 0 685.3 1 1 0 0 -QUAL 0 772.6 1 1 0 0 -QUAL 0 843.7 0 0 0 1 -# IDD, InDel distribution: -# IDD [2]id [3]length (deletions negative) [4]number of sites [5]number of genotypes [6]mean VAF -IDD 0 -60 6 0 . -IDD 0 -59 1 0 . -IDD 0 -56 1 0 . -IDD 0 -54 1 0 . -IDD 0 -50 1 0 . -IDD 0 -47 1 0 . -IDD 0 -42 1 0 . -IDD 0 -41 2 0 . -IDD 0 -40 2 0 . -IDD 0 -38 1 0 . -IDD 0 -36 2 0 . -IDD 0 -31 2 0 . -IDD 0 -30 1 0 . -IDD 0 -28 2 0 . -IDD 0 -27 4 0 . -IDD 0 -26 2 0 . -IDD 0 -25 3 0 . -IDD 0 -24 3 0 . -IDD 0 -22 1 0 . -IDD 0 -21 5 0 . -IDD 0 -20 8 0 . -IDD 0 -18 5 0 . -IDD 0 -17 3 0 . -IDD 0 -16 5 0 . -IDD 0 -15 7 0 . -IDD 0 -14 8 0 . -IDD 0 -13 7 0 . -IDD 0 -12 16 0 . -IDD 0 -11 4 0 . -IDD 0 -10 11 0 . -IDD 0 -9 6 0 . -IDD 0 -8 28 0 . -IDD 0 -7 9 0 . -IDD 0 -6 28 0 . -IDD 0 -5 31 0 . -IDD 0 -4 97 0 . -IDD 0 -3 67 0 . -IDD 0 -2 155 0 . -IDD 0 -1 430 0 . -IDD 0 1 437 0 . -IDD 0 2 181 0 . -IDD 0 3 82 0 . -IDD 0 4 95 0 . -IDD 0 5 24 0 . -IDD 0 6 30 0 . -IDD 0 7 11 0 . -IDD 0 8 34 0 . -IDD 0 9 7 0 . -IDD 0 10 19 0 . -IDD 0 11 2 0 . -IDD 0 12 16 0 . -IDD 0 13 3 0 . -IDD 0 14 7 0 . -IDD 0 15 4 0 . -IDD 0 16 7 0 . -IDD 0 17 1 0 . -IDD 0 18 2 0 . -IDD 0 19 2 0 . -IDD 0 20 3 0 . -IDD 0 22 2 0 . -IDD 0 24 5 0 . -IDD 0 26 1 0 . -IDD 0 27 1 0 . -IDD 0 28 1 0 . -IDD 0 30 1 0 . -IDD 0 32 1 0 . -IDD 0 33 3 0 . -IDD 0 34 1 0 . -IDD 0 36 3 0 . -IDD 0 38 1 0 . -IDD 0 40 2 0 . -IDD 0 41 2 0 . -IDD 0 42 3 0 . -IDD 0 44 1 0 . -IDD 0 46 1 0 . -IDD 0 59 1 0 . -IDD 0 60 13 0 . -# ST, Substitution types: -# ST [2]id [3]type [4]count -ST 0 A>C 319 -ST 0 A>G 1404 -ST 0 A>T 230 -ST 0 C>A 329 -ST 0 C>G 464 -ST 0 C>T 1505 -ST 0 G>A 1455 -ST 0 G>C 458 -ST 0 G>T 331 -ST 0 T>A 255 -ST 0 T>C 1420 -ST 0 T>G 325 -# DP, Depth distribution -# DP [2]id [3]bin [4]number of genotypes [5]fraction of genotypes (%) [6]number of sites [7]fraction of sites (%) -DP 0 2 0 0.000000 106 1.027032 -DP 0 3 0 0.000000 110 1.065788 -DP 0 4 0 0.000000 76 0.736363 -DP 0 5 0 0.000000 71 0.687918 -DP 0 6 0 0.000000 60 0.581339 -DP 0 7 0 0.000000 48 0.465071 -DP 0 8 0 0.000000 48 0.465071 -DP 0 9 0 0.000000 68 0.658851 -DP 0 10 0 0.000000 61 0.591028 -DP 0 11 0 0.000000 83 0.804186 -DP 0 12 0 0.000000 92 0.891386 -DP 0 13 0 0.000000 111 1.075477 -DP 0 14 0 0.000000 83 0.804186 -DP 0 15 0 0.000000 116 1.123922 -DP 0 16 0 0.000000 116 1.123922 -DP 0 17 0 0.000000 134 1.298324 -DP 0 18 0 0.000000 118 1.143300 -DP 0 19 0 0.000000 134 1.298324 -DP 0 20 0 0.000000 169 1.637438 -DP 0 21 0 0.000000 152 1.472726 -DP 0 22 0 0.000000 168 1.627749 -DP 0 23 0 0.000000 171 1.656816 -DP 0 24 0 0.000000 196 1.899041 -DP 0 25 0 0.000000 238 2.305978 -DP 0 26 0 0.000000 245 2.373801 -DP 0 27 0 0.000000 289 2.800116 -DP 0 28 0 0.000000 344 3.333010 -DP 0 29 0 0.000000 382 3.701192 -DP 0 30 0 0.000000 365 3.536479 -DP 0 31 0 0.000000 385 3.730259 -DP 0 32 0 0.000000 412 3.991861 -DP 0 33 0 0.000000 395 3.827149 -DP 0 34 0 0.000000 397 3.846526 -DP 0 35 0 0.000000 436 4.224397 -DP 0 36 0 0.000000 417 4.040306 -DP 0 37 0 0.000000 422 4.088751 -DP 0 38 0 0.000000 383 3.710881 -DP 0 39 0 0.000000 338 3.274876 -DP 0 40 0 0.000000 305 2.955140 -DP 0 41 0 0.000000 272 2.635404 -DP 0 42 0 0.000000 248 2.402868 -DP 0 43 0 0.000000 227 2.199399 -DP 0 44 0 0.000000 168 1.627749 -DP 0 45 0 0.000000 180 1.744017 -DP 0 46 0 0.000000 135 1.308013 -DP 0 47 0 0.000000 107 1.036721 -DP 0 48 0 0.000000 107 1.036721 -DP 0 49 0 0.000000 75 0.726674 -DP 0 50 0 0.000000 76 0.736363 -DP 0 51 0 0.000000 72 0.697607 -DP 0 52 0 0.000000 57 0.552272 -DP 0 53 0 0.000000 41 0.397248 -DP 0 54 0 0.000000 44 0.426315 -DP 0 55 0 0.000000 42 0.406937 -DP 0 56 0 0.000000 26 0.251914 -DP 0 57 0 0.000000 21 0.203469 -DP 0 58 0 0.000000 19 0.184091 -DP 0 59 0 0.000000 21 0.203469 -DP 0 60 0 0.000000 18 0.174402 -DP 0 61 0 0.000000 9 0.087201 -DP 0 62 0 0.000000 9 0.087201 -DP 0 63 0 0.000000 14 0.135646 -DP 0 64 0 0.000000 8 0.077512 -DP 0 65 0 0.000000 10 0.096890 -DP 0 66 0 0.000000 14 0.135646 -DP 0 67 0 0.000000 9 0.087201 -DP 0 68 0 0.000000 4 0.038756 -DP 0 69 0 0.000000 2 0.019378 -DP 0 70 0 0.000000 1 0.009689 -DP 0 71 0 0.000000 2 0.019378 -DP 0 72 0 0.000000 2 0.019378 -DP 0 73 0 0.000000 3 0.029067 -DP 0 74 0 0.000000 3 0.029067 -DP 0 75 0 0.000000 4 0.038756 -DP 0 76 0 0.000000 1 0.009689 -DP 0 78 0 0.000000 2 0.019378 -DP 0 79 0 0.000000 3 0.029067 -DP 0 80 0 0.000000 1 0.009689 -DP 0 81 0 0.000000 1 0.009689 -DP 0 82 0 0.000000 1 0.009689 -DP 0 84 0 0.000000 1 0.009689 -DP 0 87 0 0.000000 2 0.019378 -DP 0 90 0 0.000000 1 0.009689 -DP 0 95 0 0.000000 1 0.009689 -DP 0 104 0 0.000000 1 0.009689 -DP 0 107 0 0.000000 1 0.009689 -DP 0 109 0 0.000000 3 0.029067 -DP 0 110 0 0.000000 2 0.019378 -DP 0 111 0 0.000000 2 0.019378 -DP 0 112 0 0.000000 3 0.029067 -DP 0 113 0 0.000000 1 0.009689 diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json deleted file mode 100644 index 4e78482d..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/count_variants/NA12878-chr14-AKT1.variant_counts.json +++ /dev/null @@ -1,16 +0,0 @@ -{ - "all_indels": 1860, - "all_snps": 8512, - "pass_del": 848, - "pass_het_indels": 1103, - "pass_het_snps": 4341, - "pass_homalt_indels": 740, - "pass_homalt_snps": 4148, - "pass_indel_het_hom": 1.49, - "pass_indels": 1843, - "pass_ins": 850, - "pass_ins_del": 1.0, - "pass_snp_het_hom": 1.05, - "pass_snp_ts_tv": 2.13, - "pass_snps": 8489 -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json deleted file mode 100644 index d97f88d2..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1.metrics.json +++ /dev/null @@ -1,23 +0,0 @@ -{ - "biosample": { - "id": "NA12878-chr14-AKT1" - }, - "wgs_qc_metrics": { - "count_deletions": 848, - "count_insertions": 850, - "count_snvs": 8489, - "cross_contamination_rate": "NA", - "insert_size_std_deviation": "NA", - "mad_autosome_coverage": "NA", - "mean_autosome_coverage": "NA", - "mean_insert_size": "NA", - "pct_autosomes_15x": "NA", - "pct_reads_mapped": "NA", - "pct_reads_properly_paired": "NA", - "ratio_heterozygous_homzygous_indel": 1.49, - "ratio_heterozygous_homzygous_snv": 1.05, - "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions": 2.13, - "yield_bp_q30": "NA" - } -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log deleted file mode 100644 index 81d40fde..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc.log +++ /dev/null @@ -1,28 +0,0 @@ -[2024-05-29 09:35:42,298] multiqc [DEBUG ] This is MultiQC v1.21 -[2024-05-29 09:35:42,298] multiqc [INFO ] Expanded search patterns with the following: multiqc_npm/picard_quality_yield_metrics, multiqc_npm/samtools_stats_bq, multiqc_npm/count_variants -[2024-05-29 09:35:42,299] multiqc [DEBUG ] Command used: /usr/local/conda/bin/multiqc --data-format json --enable-npm-plugin -o NA12878-chr14-AKT1 . -[2024-05-29 09:35:43,009] multiqc [WARNING] MultiQC Version v1.22.1 now available! -[2024-05-29 09:35:43,009] multiqc [DEBUG ] Latest MultiQC version is v1.22.1, released 2024-05-17 -[2024-05-29 09:35:43,009] multiqc [DEBUG ] Working dir : /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191 -[2024-05-29 09:35:43,009] multiqc [DEBUG ] Template : default -[2024-05-29 09:35:43,009] multiqc [DEBUG ] Running Python 3.12.2 | packaged by conda-forge | (main, Feb 16 2024, 20:50:58) [GCC 12.3.0] -[2024-05-29 09:35:43,010] multiqc [DEBUG ] Analysing modules: multiqc_npm, custom_content, ccs, ngsderive, purple, conpair, isoseq, lima, peddy, somalier, methylqa, mosdepth, phantompeakqualtools, qualimap, bamdst, preseq, hifiasm, quast, qorts, rna_seqc, rockhopper, rsem, rseqc, busco, bustools, goleft_indexcov, gffcompare, disambiguate, supernova, deeptools, sargasso, verifybamid, mirtrace, happy, mirtop, sambamba, gopeaks, homer, hops, macs2, theta2, snpeff, gatk, htseq, bcftools, featureCounts, fgbio, dragen, dragen_fastqc, dedup, pbmarkdup, damageprofiler, mapdamage, biobambam2, jcvi, mtnucratio, picard, vep, bakta, prokka, qc3C, nanostat, samblaster, samtools, sexdeterrmine, seqera_cli, eigenstratdatabasetools, bamtools, jellyfish, vcftools, longranger, stacks, varscan2, snippy, umitools, truvari, megahit, bbmap, bismark, biscuit, diamond, hicexplorer, hicup, hicpro, salmon, kallisto, slamdunk, star, hisat2, tophat, bowtie2, bowtie1, cellranger, snpsplit, odgi, pangolin, nextclade, freyja, humid, kat, leehom, librarian, nonpareil, adapterRemoval, bbduk, clipandmerge, cutadapt, flexbar, sourmash, kaiju, bracken, kraken, malt, motus, trimmomatic, sickle, skewer, sortmerna, biobloomtools, fastq_screen, afterqc, fastp, fastqc, filtlong, prinseqplusplus, pychopper, porechop, pycoqc, minionqc, anglerfish, multivcfanalyzer, clusterflow, checkqc, bcl2fastq, bclconvert, interop, ivar, flash, seqyclean, optitype, whatshap, spaceranger, xenome, xengsort, metaphlan, seqwho -[2024-05-29 09:35:43,010] multiqc [DEBUG ] Using temporary directory for creating report: /tmp/tmp_6i2nysw -[2024-05-29 09:35:43,010] multiqc [INFO ] Search path : /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191 -[2024-05-29 09:35:43,050] multiqc [DEBUG ] Summary of files that were skipped by the search: [skipped_module_specific_max_filesize: 16] // [skipped_no_match: 7] -[2024-05-29 09:35:44,035] multiqc [INFO ] Running with MultiQC NPM plugin -[2024-05-29 09:35:44,036] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_npm_count_variants.json -[2024-05-29 09:35:44,036] multiqc [INFO ] Found 1 npm_count_variants reports -[2024-05-29 09:35:44,187] multiqc [DEBUG ] No samples found: custom_content -[2024-05-29 09:35:44,194] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_bcftools_stats.json -[2024-05-29 09:35:44,301] multiqc.modules.bcftools.bcftools [INFO ] Found 1 stats reports -[2024-05-29 09:35:44,301] multiqc.utils.software_versions [DEBUG ] Reading software versions from config.software_versions -[2024-05-29 09:35:44,302] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_software_versions.json -[2024-05-29 09:35:44,312] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_general_stats.json -[2024-05-29 09:35:44,313] multiqc [DEBUG ] Compressing plot data -[2024-05-29 09:35:44,356] multiqc.utils.util_functions [DEBUG ] Wrote data file multiqc_data.json -[2024-05-29 09:35:44,356] multiqc [INFO ] Report : NA12878-chr14-AKT1/multiqc_report.html -[2024-05-29 09:35:44,356] multiqc [INFO ] Data : NA12878-chr14-AKT1/multiqc_data -[2024-05-29 09:35:44,356] multiqc [DEBUG ] Moving data file from '/tmp/tmp_6i2nysw/multiqc_data' to '/home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191/NA12878-chr14-AKT1/multiqc_data' -[2024-05-29 09:35:44,358] multiqc [DEBUG ] Full report path: /home/ubuntu/April-7/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d4/01e07332ad951f29eae56a30653191/NA12878-chr14-AKT1/multiqc_report.html -[2024-05-29 09:35:44,466] multiqc [INFO ] MultiQC complete diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json deleted file mode 100644 index df7371bb..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_bak/results/multiqc/NA12878-chr14-AKT1/multiqc_data/multiqc_bcftools_stats.json +++ /dev/null @@ -1,31 +0,0 @@ -{ - "NA12878-chr14-AKT1": { - 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              - Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
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              NA12878-chr14-AKT1
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              Bcftools

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              - - - Version: 1.15.1 - - - - HTSlib: 1.19 - - -
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              Bcftools contains utilities for variant calling and manipulating VCFs and BCFs.DOI: 10.1093/gigascience/giab008.

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              - Variant depths - -

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              Read depth support distribution for called variants

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              Software Versions

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              Software Versions lists versions of software tools extracted from file contents.

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              GroupSoftwareVersion
              BcftoolsBcftools1.15.1
              HTSlib1.19
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              - - - MultiQC v1.21 - - - Written by Phil Ewels, - available on GitHub. -

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              - This report uses HighCharts, - jQuery, - jQuery UI, - Bootstrap, - FileSaver.js and - clipboard.js. -

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              - - - - - - - - - - - - - - From 21d96840b136176e2db3639e5e603fdb61b1f4bb Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 02:43:30 +0000 Subject: [PATCH 092/132] remove picard collectvariantcalling metrics --- nextflow.config | 22 +++++++++++----------- 1 file changed, 11 insertions(+), 11 deletions(-) diff --git a/nextflow.config b/nextflow.config index 555b2c28..2b00e4e8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -91,17 +91,17 @@ process { mode: params.publish_mode, ] } - withName:picard_collect_variant_calling_metrics_vcf { - - cpus = 4 - disk = '50 GB' - memory = '2 GB' - - publishDir = [ - path: "${params.publishdir}/CollectVariantCallingMetrics", - mode: params.publish_mode, - ] - } +// withName:picard_collect_variant_calling_metrics_vcf { +// +// cpus = 4 +// disk = '50 GB' +// memory = '2 GB' +// +// publishDir = [ +// path: "${params.publishdir}/CollectVariantCallingMetrics", +// mode: params.publish_mode, +// ] +// } withName:count_variants { cpus = 16 disk = '50 GB' From eda892325e2551a05be34cc8d7b22facfa10cc11 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 02:44:10 +0000 Subject: [PATCH 093/132] channel desc --- main.nf | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index 2e3dd1be..5df7fb50 100644 --- a/main.nf +++ b/main.nf @@ -92,12 +92,11 @@ include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_bam include { picard_collect_multiple_metrics as picard_collect_multiple_metrics_cram } from './modules/CollectMultipleMetrics' include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_bam } from './modules/CollectWgsMetrics' include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from './modules/CollectWgsMetrics' -include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' +// include { picard_collect_variant_calling_metrics_vcf } from './modules/CollectVariantCallingMetrics' include { bcftools_stats } from './modules/bcftools' include { count_variants } from './modules/count_variants' include { count_aln } from './modules/count_aln' include { compile_aln_vcf } from './modules/compile_aln_vcf' -// include { multiqc } from './modules/multiqc' /* ---------------------------------------------------------------------- @@ -125,6 +124,7 @@ workflow { .ifEmpty { ['biosample_id': params.biosample_id, 'aln': params.aln] } .set { samples } +// Create channel branches for bam and cram input type Channel samples.branch { rec -> def aln_file = rec.aln ? file( rec.aln ) : null @@ -141,7 +141,7 @@ workflow { } .set { aln_inputs } - +// Conditional input option to compile metrics if both input aln and vcf given Channel samples.branch { rec -> def aln_file = rec.aln ? file( rec.aln ) : null @@ -183,6 +183,7 @@ workflow { //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) +// Channel to get sample id mapping Channel samples.map { it.biosample_id } .set { sample_ids } From 890f706a2310f4bba17962238f54da12b6e36c44 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 05:23:46 +0000 Subject: [PATCH 094/132] update vcf qc desc and implementaion details on metrics.py --- bin/metrics/metrics.py | 62 ++++++++++-------------------------------- 1 file changed, 14 insertions(+), 48 deletions(-) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index cafbe7b7..64951d1c 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -160,9 +160,9 @@ def cross_contamination_rate(mqc, biosample_id): def count_snvs(mqc, biosample_id): """ - The number of PASS SNPs. + Description: The number of variant type SNVs in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view. (bcftools view -H -v snps -f PASS) """ k = inspect.currentframe().f_code.co_name @@ -175,45 +175,11 @@ def count_snvs(mqc, biosample_id): return k, v -def pass_het_snps(mqc, biosample_id): - """ - The number of PASS het SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_het_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pass_homalt_snps(mqc, biosample_id): - """ - The number of PASS hom alt SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_homalt_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - def ratio_heterozygous_homzygous_snv(mqc, biosample_id): """ - The het/hom ratio for PASS SNPs. + Description: The ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v snps -f PASS -g het / bcftools view -H -v snps -f PASS -g hom). """ k = inspect.currentframe().f_code.co_name @@ -228,9 +194,9 @@ def ratio_heterozygous_homzygous_snv(mqc, biosample_id): def ratio_heterozygous_homzygous_indel(mqc, biosample_id): """ - The het/hom ratio for PASS INDELs. + Description: The ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS -g het / bcftools view -H -v indels -f PASS -g hom). """ k = inspect.currentframe().f_code.co_name @@ -245,9 +211,9 @@ def ratio_heterozygous_homzygous_indel(mqc, biosample_id): def count_deletions(mqc, biosample_id): """ - The number of PASS deletions. + Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS....INS/bcftools view -H -v indels -f PASS....DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered. """ k = inspect.currentframe().f_code.co_name @@ -262,9 +228,9 @@ def count_deletions(mqc, biosample_id): def count_insertions(mqc, biosample_id): """ - The number of PASS insertions. + Description: The number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS....INS). Insertions are only considered in this metric short, less than 50bp, insertions as commonly identified by most short reads variant callers. Structural variations which include insertions larger than 50bp and which are typically identified using dedicated SV callers are not considered. """ k = inspect.currentframe().f_code.co_name @@ -279,9 +245,9 @@ def count_insertions(mqc, biosample_id): def ratio_insertion_deletion(mqc, biosample_id): """ - The ins/del ratio for PASS INDELs. + Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools view) + Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS....INS/bcftools view -H -v indels -f PASS....DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered. """ k = inspect.currentframe().f_code.co_name @@ -297,9 +263,9 @@ def ratio_insertion_deletion(mqc, biosample_id): def ratio_transitions_transversions(mqc, biosample_id): """ - The transition to transversion ratio of passing bi-allelic SNPs. + Description: The ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality variants. - Source: count_variants.py (bcftools stats - tstv) + Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality-variants by bcftools stats, (bcftools stats -f PASS ... TSTV). """ k = inspect.currentframe().f_code.co_name From 7571cb5b3b354cf1d3cba9d2d4ddc84f15b4f7cf Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:13:27 +0000 Subject: [PATCH 095/132] revert back the 1000genome s3 path to params.yaml --- .../NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml | 1 - .../params.yaml.aln | 4 ++++ .../params.yaml.aln.vcf | 5 +++++ .../params.yaml.vcf | 4 ++++ tests/NA12878_1000genomes-dragen-3.7.6/params.yaml | 4 +++- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln | 4 ---- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf | 5 ----- .../{params.yaml.BAK => params.yaml.aln-vcf.bak} | 0 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak | 5 +++++ tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf | 4 ---- tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak | 5 +++++ tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 2 +- 12 files changed, 27 insertions(+), 16 deletions(-) create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf rename tests/NA12878_1000genomes-dragen-3.7.6/{params.yaml.BAK => params.yaml.aln-vcf.bak} (100%) create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml index 5e6ea776..d629dab9 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml @@ -1,5 +1,4 @@ samples: - biosample_id: NA12878-chr14-AKT1 - aln: NA12878-chr14-AKT1.bam vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln new file mode 100644 index 00000000..780599e3 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln @@ -0,0 +1,4 @@ +samples: +- + biosample_id: NA12878-chr14-AKT1 + aln: NA12878-chr14-AKT1.bam diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf new file mode 100644 index 00000000..5e6ea776 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf @@ -0,0 +1,5 @@ +samples: +- + biosample_id: NA12878-chr14-AKT1 + aln: NA12878-chr14-AKT1.bam + vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf new file mode 100644 index 00000000..d629dab9 --- /dev/null +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf @@ -0,0 +1,4 @@ +samples: +- + biosample_id: NA12878-chr14-AKT1 + vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml index d12f9e77..f55939d8 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml @@ -1,4 +1,6 @@ samples: - biosample_id: NA12878 - vcf: /data/NA12878.hard-filtered.vcf.gz + aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam + vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz +aws_no_sign_request: true diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln deleted file mode 100644 index f02876a7..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln +++ /dev/null @@ -1,4 +0,0 @@ -samples: -- - biosample_id: NA12878 - aln: /data/NA12878.bam diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf deleted file mode 100644 index 2d0e3cb4..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf +++ /dev/null @@ -1,5 +0,0 @@ -samples: -- - biosample_id: NA12878 - aln: /data/NA12878.bam - vcf: /data/NA12878.hard-filtered.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.BAK rename to tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak new file mode 100644 index 00000000..dd73cf16 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak @@ -0,0 +1,5 @@ +samples: +- + biosample_id: NA12878 + aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam +aws_no_sign_request: true diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf deleted file mode 100644 index d12f9e77..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf +++ /dev/null @@ -1,4 +0,0 @@ -samples: -- - biosample_id: NA12878 - vcf: /data/NA12878.hard-filtered.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak new file mode 100644 index 00000000..5965de59 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak @@ -0,0 +1,5 @@ +samples: +- + biosample_id: NA12878 + vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz +aws_no_sign_request: true diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 68ac94d1..00cddcff 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -6,7 +6,7 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/home/ubuntu/bin/nf23.09.1-edge-all/nextflow run ../../main.nf \ +/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ From b6377b53520bf4e9a8b2c3b52eaa4d46d1ae799b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:15:49 +0000 Subject: [PATCH 096/132] Add results of metrics json --- .../output_certified/NA12878.aln.metrics.json | 16 +++++++++++++ .../output_certified/NA12878.metrics.json | 23 +++++++++++++++++++ .../NA12878.variant.metrics.json | 14 +++++++++++ 3 files changed, 53 insertions(+) create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json create mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json new file mode 100644 index 00000000..eb94cdbf --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json @@ -0,0 +1,16 @@ +{ + "biosample": { + "id": "NA12878" + }, + "wgs_qc_metrics": { + "cross_contamination_rate": "1.8379e-06", + "insert_size_std_deviation": "98.7", + "mad_autosome_coverage": "4", + "mean_autosome_coverage": "31.080137", + "mean_insert_size": "439.8", + "pct_autosomes_15x": "0.959502", + "pct_reads_mapped": "99.76", + "pct_reads_properly_paired": "98.1", + "yield_bp_q30": "110066894077" + } +} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json new file mode 100644 index 00000000..d1ac457c --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json @@ -0,0 +1,23 @@ +{ + "biosample": { + "id": "NA12878" + }, + "wgs_qc_metrics": { + "count_deletions": 433328, + "count_insertions": 419222, + "count_snvs": 3904186, + "cross_contamination_rate": "1.8379e-06", + "insert_size_std_deviation": "98.7", + "mad_autosome_coverage": "4", + "mean_autosome_coverage": "31.080137", + "mean_insert_size": "439.8", + "pct_autosomes_15x": "0.959502", + "pct_reads_mapped": "99.76", + "pct_reads_properly_paired": "98.1", + "ratio_heterozygous_homzygous_indel": 1.96, + "ratio_heterozygous_homzygous_snv": 1.57, + "ratio_insertion_deletion": 0.97, + "ratio_transitions_transversions_snv": 1.96, + "yield_bp_q30": "110066894077" + } +} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json new file mode 100644 index 00000000..26ec1554 --- /dev/null +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json @@ -0,0 +1,14 @@ +{ + "biosample": { + "id": "NA12878" + }, + "wgs_qc_metrics": { + "count_deletions": 433328, + "count_insertions": 419222, + "count_snvs": 3904186, + "ratio_heterozygous_homzygous_indel": 1.96, + "ratio_heterozygous_homzygous_snv": 1.57, + "ratio_insertion_deletion": 0.97, + "ratio_transitions_transversions_snv": 1.96 + } +} From ba306a05e9d8269b85acb697414d06c81a5056b5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:18:58 +0000 Subject: [PATCH 097/132] update gitignore --- .gitignore | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/.gitignore b/.gitignore index b66ba3a9..6b03b937 100644 --- a/.gitignore +++ b/.gitignore @@ -18,6 +18,10 @@ tests/*/output_certified/results/mosdepth tests/*/output_certified/results/samtools tests/*/output_certified/results/picard tests/*/output_certified/results/verifybamid2 +tests/*/output_certified/picard_collect_multiple_metrics +tests/*/output_certified/picard_collect_wgs_metrics +tests/*/output_certified/bcftools + # MacOS **/.DS* From 54eecb5538539ea09dd2520ff43130dde054033e Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:21:40 +0000 Subject: [PATCH 098/132] Add results of metrics json --- .../output_certified/NA12878.metrics.json | 23 ----------------- .../metrics}/NA12878.aln.metrics.json | 0 .../results/metrics/NA12878.metrics.json | 25 ++++++++++++------- .../metrics}/NA12878.variant.metrics.json | 0 4 files changed, 16 insertions(+), 32 deletions(-) delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json rename tests/NA12878_1000genomes-dragen-3.7.6/output_certified/{ => results/metrics}/NA12878.aln.metrics.json (100%) rename tests/NA12878_1000genomes-dragen-3.7.6/output_certified/{ => results/metrics}/NA12878.variant.metrics.json (100%) diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json deleted file mode 100644 index d1ac457c..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.metrics.json +++ /dev/null @@ -1,23 +0,0 @@ -{ - "biosample": { - "id": "NA12878" - }, - "wgs_qc_metrics": { - "count_deletions": 433328, - "count_insertions": 419222, - "count_snvs": 3904186, - "cross_contamination_rate": "1.8379e-06", - "insert_size_std_deviation": "98.7", - "mad_autosome_coverage": "4", - "mean_autosome_coverage": "31.080137", - "mean_insert_size": "439.8", - "pct_autosomes_15x": "0.959502", - "pct_reads_mapped": "99.76", - "pct_reads_properly_paired": "98.1", - "ratio_heterozygous_homzygous_indel": 1.96, - "ratio_heterozygous_homzygous_snv": 1.57, - "ratio_insertion_deletion": 0.97, - "ratio_transitions_transversions_snv": 1.96, - "yield_bp_q30": "110066894077" - } -} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.aln.metrics.json rename to tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json index bb4e40a6..d1ac457c 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json @@ -3,14 +3,21 @@ "id": "NA12878" }, "wgs_qc_metrics": { - "cross_contamination_rate": 1.8379e-06, - "insert_size_std_deviation": 98.7, - "mad_autosome_coverage": 2.41, - "mean_autosome_coverage": 31.5975, - "mean_insert_size": 439.8, - "pct_autosomes_15x": 95.91997, - "pct_reads_mapped": 99.76052, - "pct_reads_properly_paired": 98.09972, - "yield_bp_q30": 110066894077 + "count_deletions": 433328, + "count_insertions": 419222, + "count_snvs": 3904186, + "cross_contamination_rate": "1.8379e-06", + "insert_size_std_deviation": "98.7", + "mad_autosome_coverage": "4", + "mean_autosome_coverage": "31.080137", + "mean_insert_size": "439.8", + "pct_autosomes_15x": "0.959502", + "pct_reads_mapped": "99.76", + "pct_reads_properly_paired": "98.1", + "ratio_heterozygous_homzygous_indel": 1.96, + "ratio_heterozygous_homzygous_snv": 1.57, + "ratio_insertion_deletion": 0.97, + "ratio_transitions_transversions_snv": 1.96, + "yield_bp_q30": "110066894077" } } diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/output_certified/NA12878.variant.metrics.json rename to tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json From fb5d5ce08d6e3a75d554121e1d647fc3857a92be Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:22:36 +0000 Subject: [PATCH 099/132] Add results of metrics json --- .../results/metrics/NA12877.metrics.json | 16 ---------------- 1 file changed, 16 deletions(-) delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12877.metrics.json diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12877.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12877.metrics.json deleted file mode 100644 index d1f18db4..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12877.metrics.json +++ /dev/null @@ -1,16 +0,0 @@ -{ - "biosample": { - "id": "NA12877" - }, - "wgs_qc_metrics": { - "cross_contamination_rate": 6.27435e-10, - "insert_size_std_deviation": 103.6, - "mad_autosome_coverage": 2.68, - "mean_autosome_coverage": 33.71467, - "mean_insert_size": 458.4, - "pct_autosomes_15x": 96.01937, - "pct_reads_mapped": 99.7996, - "pct_reads_properly_paired": 98.1512, - "yield_bp_q30": 110850191617 - } -} From be420a8e6150000392869841c4d487eb35d9c04a Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 4 Sep 2024 06:26:15 +0000 Subject: [PATCH 100/132] Add results of metrics json --- .../NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml | 1 + .../params.yaml.aln.vcf | 5 ----- .../{params.yaml.aln.bak => params.yaml.aln} | 0 .../params.yaml.aln-vcf.bak | 6 ------ .../{params.yaml.vcf.bak => params.yaml.vcf} | 0 5 files changed, 1 insertion(+), 11 deletions(-) delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf rename tests/NA12878_1000genomes-dragen-3.7.6/{params.yaml.aln.bak => params.yaml.aln} (100%) delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak rename tests/NA12878_1000genomes-dragen-3.7.6/{params.yaml.vcf.bak => params.yaml.vcf} (100%) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml index d629dab9..5e6ea776 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml @@ -1,4 +1,5 @@ samples: - biosample_id: NA12878-chr14-AKT1 + aln: NA12878-chr14-AKT1.bam vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf deleted file mode 100644 index 5e6ea776..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln.vcf +++ /dev/null @@ -1,5 +0,0 @@ -samples: -- - biosample_id: NA12878-chr14-AKT1 - aln: NA12878-chr14-AKT1.bam - vcf: NA12878-chr14-AKT1.vcf.gz diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln.bak rename to tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak deleted file mode 100644 index f55939d8..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln-vcf.bak +++ /dev/null @@ -1,6 +0,0 @@ -samples: -- - biosample_id: NA12878 - aln: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.bam - vcf: s3://1000genomes-dragen-3.7.6/data/individuals/hg38-graph-based/NA12878/NA12878.hard-filtered.vcf.gz -aws_no_sign_request: true diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak b/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf.bak rename to tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf From ea35cddc0bf650ab416774326a940fb1c923eeaf Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 9 Sep 2024 06:46:10 +0000 Subject: [PATCH 101/132] code cleanup main.nf --- main.nf | 57 --------------------------------------------------------- 1 file changed, 57 deletions(-) diff --git a/main.nf b/main.nf index 5df7fb50..b7c3ced7 100644 --- a/main.nf +++ b/main.nf @@ -237,63 +237,6 @@ workflow { compile_aln_vcf ( ch_count ) -/* -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - ch_bam.mix(ch_cram) - .combine(vcf_qc,by:0) - //.view() - .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats, insertsize, quality, wgs_coverage, freemix ?: [], count_variants, bcftools_stats ] } - //.view() - .set { multiqc_in } -*/ - -/* -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - ch_bam.mix(ch_cram) // .ifEmpty([]) - //.combine(vcf_qc,by:0) - .join(vcf_qc, remainder: true) - //.map { it.minus(null) } - //.map { files -> files - null } - //.flatten() - .map { sample, stats, insertsize, quality, wgs_coverage, freemix, count_variants, bcftools_stats -> [ sample, stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [], count_variants ?: [], bcftools_stats ?: [] ] } - .view() - .set { multiqc_in } -*/ - - - -/* -// channel to mix the bam/cram process outputs and map the verifybamid2 'null' to '[]' if the verifybamid2 process output is empty - Channel - .empty() - sample_ids - //.combine(vcf_qc,by:0).ifEmpty([]) - //ch_bam.mix(ch_cram).ifEmpty([]) - .join(count_variants.out) - .join(bcftools_stats.out) - .map { sample, count_variants, bcftools_stats -> sample, count_variants, bcftools_stats } - .mix(ch_bam,ch_cram) - //.combine(vcf_qc,by:0).ifEmpty([]) - //.join(vcf_qc) - //.view() - //.mix(vcf_qc).ifEmpty([]) - //.join(vcf_qc).ifEmpty([]) - //.combine(vcf_qc,by:0).ifEmpty([]) - //.flatten() - //.toList() - .view() - //.map { sample, count_variants, bcftools_stats, sample1, stats, insertsize, quality, wgs_coverage, freemix -> [ sample ?: [], count_variants ?: [], bcftools_stats ?: [], sample1 ?: [], stats ?: [], insertsize ?: [], quality ?: [], wgs_coverage ?: [], freemix ?: [] ] } - //.view() - .set { multiqc_in } -*/ - -/* - metrics_combine = Channel.empty() - if (params.vcf && params.aln) { - metrics_combine = ( count_variants.out ) - .view() - } -*/ } From 5d78a7de1b06471fe7c06fe2b2358f6fdbe126c5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 9 Sep 2024 07:21:46 +0000 Subject: [PATCH 102/132] try fixing pct_autosome_cov --- modules/CollectWgsMetrics/main.nf | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index 39899761..a23dbbc0 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -25,6 +25,10 @@ process picard_collect_wgs_metrics { cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t"\$1}' > "${sample}_wgs_metrics.metrics" cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t"\$1}' >> "${sample}_wgs_metrics.metrics" + #15x_pct=\$(cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk 'BEGIN { printf ("%.2f", 100 * \$1) }') + #15x=\$(cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X") + #15x_pct=\$(awk -v pct=\$15x 'BEGIN { printf ("%.2f", 100 * pct) }') + #echo "pct_autosomes_15x\t"\$15x >> "${sample}_wgs_metrics.metrics" cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" """ } From 08e3f01b644ca32ac815c42f543cae4af5239975 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 10 Sep 2024 04:57:53 +0000 Subject: [PATCH 103/132] update to print pct with float --- modules/CollectWgsMetrics/main.nf | 15 +++++++++------ 1 file changed, 9 insertions(+), 6 deletions(-) diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index a23dbbc0..ceaa4d13 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -23,12 +23,15 @@ process picard_collect_wgs_metrics { INTERVALS=${autosomes_non_gap_regions} \ VALIDATION_STRINGENCY=SILENT - cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t"\$1}' > "${sample}_wgs_metrics.metrics" - cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk '{print "pct_autosomes_15x\t"\$1}' >> "${sample}_wgs_metrics.metrics" - #15x_pct=\$(cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X" | awk 'BEGIN { printf ("%.2f", 100 * \$1) }') - #15x=\$(cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | grep -v "PCT_15X") - #15x_pct=\$(awk -v pct=\$15x 'BEGIN { printf ("%.2f", 100 * pct) }') - #echo "pct_autosomes_15x\t"\$15x >> "${sample}_wgs_metrics.metrics" + #cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | grep -v "MEAN_COVERAGE" | awk '{print "mean_autosome_coverage\t"\$1}' > "${sample}_wgs_metrics.metrics" + avg=\$(cut -f2 "${sample}_wgs_metrics.txt" | grep "MEAN_COVERAGE" -A 1 | tail -n 1) + avgcov=\$(awk -v avgval=\$avg 'BEGIN { printf ("%.2f", avgval) }') + echo "mean_autosome_coverage\t"\$avgcov >> "${sample}_wgs_metrics.metrics" + + cov=\$(cut -f17 NA12878-chr14-AKT1_wgs_metrics.txt | grep "PCT_15X" -A 1 | tail -n 1) + covpct=\$(awk -v covval=\$cov 'BEGIN { printf ("%.2f", 100 * covval) }') + echo "pct_autosomes_15x\t"\$covpct >> "${sample}_wgs_metrics.metrics" + cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" """ } From 6e3b5dbb74536fd531c36ced33a39aa6ad6640bd Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 10 Sep 2024 11:17:27 +0000 Subject: [PATCH 104/132] update to print pct with float --- modules/CollectWgsMetrics/main.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index ceaa4d13..ed7ab75f 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -30,6 +30,7 @@ process picard_collect_wgs_metrics { cov=\$(cut -f17 NA12878-chr14-AKT1_wgs_metrics.txt | grep "PCT_15X" -A 1 | tail -n 1) covpct=\$(awk -v covval=\$cov 'BEGIN { printf ("%.2f", 100 * covval) }') + echo "pct_autosomes_15x\t"\$cov >> "${sample}_wgs_metrics.metrics" echo "pct_autosomes_15x\t"\$covpct >> "${sample}_wgs_metrics.metrics" cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" From fea3ee002c2a33738562f4c539b0ccdf78980734 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 10 Sep 2024 23:09:06 +0000 Subject: [PATCH 105/132] update to print pct with float --- main.nf | 2 ++ modules/CollectWgsMetrics/main.nf | 3 +-- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index b7c3ced7..66bc8446 100644 --- a/main.nf +++ b/main.nf @@ -188,6 +188,7 @@ workflow { samples.map { it.biosample_id } .set { sample_ids } +/* // channel for samplelist vcf input file processed outputs Channel .empty() @@ -195,6 +196,7 @@ workflow { .join( count_variants.out ) .join( bcftools_stats.out ) .set { vcf_qc } +*/ // channel for samplelist input file type bam processed outputs Channel diff --git a/modules/CollectWgsMetrics/main.nf b/modules/CollectWgsMetrics/main.nf index ed7ab75f..d849f3b2 100644 --- a/modules/CollectWgsMetrics/main.nf +++ b/modules/CollectWgsMetrics/main.nf @@ -28,9 +28,8 @@ process picard_collect_wgs_metrics { avgcov=\$(awk -v avgval=\$avg 'BEGIN { printf ("%.2f", avgval) }') echo "mean_autosome_coverage\t"\$avgcov >> "${sample}_wgs_metrics.metrics" - cov=\$(cut -f17 NA12878-chr14-AKT1_wgs_metrics.txt | grep "PCT_15X" -A 1 | tail -n 1) + cov=\$(cut -f17 "${sample}_wgs_metrics.txt" | grep "PCT_15X" -A 1 | tail -n 1) covpct=\$(awk -v covval=\$cov 'BEGIN { printf ("%.2f", 100 * covval) }') - echo "pct_autosomes_15x\t"\$cov >> "${sample}_wgs_metrics.metrics" echo "pct_autosomes_15x\t"\$covpct >> "${sample}_wgs_metrics.metrics" cut -f5 "${sample}_wgs_metrics.txt" | grep "MAD_COVERAGE" -A 1 | grep -v "MAD_COVERAGE" | awk '{print "mad_autosome_coverage\t"\$1}' >> "${sample}_wgs_metrics.metrics" From b79a7e8ef9d962e7924b7c9ee718873a97e059f6 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 15 Sep 2024 02:24:38 +0000 Subject: [PATCH 106/132] clean backup files --- bin/metrics/metrics.py.BAK | 313 -------------------------------- bin/metrics/metrics.py.UPDATE | 330 ---------------------------------- containers/Dockerfile.BAK | 75 -------- 3 files changed, 718 deletions(-) delete mode 100644 bin/metrics/metrics.py.BAK delete mode 100644 bin/metrics/metrics.py.UPDATE delete mode 100644 containers/Dockerfile.BAK diff --git a/bin/metrics/metrics.py.BAK b/bin/metrics/metrics.py.BAK deleted file mode 100644 index cafbe7b7..00000000 --- a/bin/metrics/metrics.py.BAK +++ /dev/null @@ -1,313 +0,0 @@ -#!/usr/bin/env python3 - -import numpy as np -import inspect - -DECIMALS = 5 - -def yield_bp_q30(mqc, biosample_id): - """ - Description: The number of bases in short paired-end sequencing high quality reads, primary alignments, achieving a base quality score of 30 or greater (Phred scale). Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using GATK Picard CollectQualityYieldMetrics, reporting the PF_Q30_BASES field. Only high quality bases from primary alignments are considered. No filter on duplicated reads, clipped bases or mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_picard_QualityYieldMetrics"][biosample_id] - v = d["PF_Q30_BASES"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pct_reads_mapped(mqc, biosample_id): - """ - Description: The percentage of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = np.divide(d["reads_mapped"], - d["sequences"]) - v = np.round(v*100, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def pct_reads_properly_paired(mqc, biosample_id): - """ - Description: The percentage of short paired-end sequencing high quality, properly paired reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of properly paired reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["reads_properly_paired_percent"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mean_autosome_coverage(mqc, biosample_id): - """ - Description: The mean sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing mean coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["MEAN_COVERAGE"] - v = np.round(np.float64(v), DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mad_autosome_coverage(mqc, biosample_id): - """ - Description: The median absolute deviation of sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing median absolute deviation coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["MAD_COVERAGE"] - v = np.round(np.float64(v), DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def pct_autosomes_15x(mqc, biosample_id): - """ - Description: The percentage of bases attaining at least 15X sequencing coverage in short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing coverage percentage of bases attaining at least 15X of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["PCT_15X"] - v = np.round(v*100, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mean_insert_size(mqc, biosample_id): - """ - Description: The mean insert size of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_average field. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["insert_size_average"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def insert_size_std_deviation(mqc, biosample_id): - """ - Description: The insert size standard deviation of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_standard_deviation field. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["insert_size_standard_deviation"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def cross_contamination_rate(mqc, biosample_id): - """ - Description: Estimation of inter-sample contamination rate of short paired-end sequencing high quality, non duplicated reads, primary alignments, mapped on GRCh38 assembly. No minimum mapping quality is imposed. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads and clipped bases. - - Implementation details: The estimation of inter-sample DNA contamination of short paired-end sequencing high quality, aligned sequence reads (BAM/CRAM) mapped on GRCh38 assembly with pre-calculated reference panel of 1000 Genome Project dataset from the VerifyBamID resource using VerifyBamID2 with NumPC “4” (# of Principal Components used in estimation), the key information “FREEMIX” in “.selfSM” in the results indicates the estimated contamination level. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_verifybamid"][biosample_id] - v = d["FREEMIX"] - except KeyError: - v = "NA" - - return k, v - -def count_snvs(mqc, biosample_id): - """ - The number of PASS SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pass_het_snps(mqc, biosample_id): - """ - The number of PASS het SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_het_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pass_homalt_snps(mqc, biosample_id): - """ - The number of PASS hom alt SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_homalt_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def ratio_heterozygous_homzygous_snv(mqc, biosample_id): - """ - The het/hom ratio for PASS SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snp_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def ratio_heterozygous_homzygous_indel(mqc, biosample_id): - """ - The het/hom ratio for PASS INDELs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_indel_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def count_deletions(mqc, biosample_id): - """ - The number of PASS deletions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_del"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def count_insertions(mqc, biosample_id): - """ - The number of PASS insertions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def ratio_insertion_deletion(mqc, biosample_id): - """ - The ins/del ratio for PASS INDELs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins_del"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - - -def ratio_transitions_transversions(mqc, biosample_id): - """ - The transition to transversion ratio of passing bi-allelic SNPs. - - Source: count_variants.py (bcftools stats - tstv) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snp_ts_tv"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v diff --git a/bin/metrics/metrics.py.UPDATE b/bin/metrics/metrics.py.UPDATE deleted file mode 100644 index b09fd78a..00000000 --- a/bin/metrics/metrics.py.UPDATE +++ /dev/null @@ -1,330 +0,0 @@ -#!/usr/bin/env python3 - -import numpy as np -import inspect - -DECIMALS = 5 - -def yield_bp_q30(mqc, biosample_id): - """ - Description: The number of bases in short paired-end sequencing high quality reads, primary alignments, achieving a base quality score of 30 or greater (Phred scale). Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using GATK Picard CollectQualityYieldMetrics, reporting the PF_Q30_BASES field. Only high quality bases from primary alignments are considered. No filter on duplicated reads, clipped bases or mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_picard_QualityYieldMetrics"][biosample_id] - v = d["PF_Q30_BASES"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pct_reads_mapped(mqc, biosample_id): - """ - Description: The percentage of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = np.divide(d["reads_mapped"], - d["sequences"]) - v = np.round(v*100, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def pct_reads_properly_paired(mqc, biosample_id): - """ - Description: The percentage of short paired-end sequencing high quality, properly paired reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the percentage of properly paired reads mapped on GRCh38 assembly. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["reads_properly_paired_percent"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mean_autosome_coverage(mqc, biosample_id): - """ - Description: The mean sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing mean coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["MEAN_COVERAGE"] - v = np.round(np.float64(v), DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mad_autosome_coverage(mqc, biosample_id): - """ - Description: The median absolute deviation of sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing median absolute deviation coverage of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["MAD_COVERAGE"] - v = np.round(np.float64(v), DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def pct_autosomes_15x(mqc, biosample_id): - """ - Description: The percentage of bases attaining at least 15X sequencing coverage in short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly. Overlapping bases are counted only once. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads. - - Implementation details: In the NPM-sample-QC reference implementation, the genome-wide sequencing coverage percentage of bases attaining at least 15X of the non gap regions of GRCh38 assembly, autosomes only, non duplicated reads, non overlapping bases, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater is derived from picard (2.27.0) CollectWgsMetrics. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_picard_wgsmetrics"][biosample_id] - v = d["PCT_15X"] - v = np.round(v*100, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def mean_insert_size(mqc, biosample_id): - """ - Description: The mean insert size of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_average field. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["insert_size_average"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def insert_size_std_deviation(mqc, biosample_id): - """ - Description: The insert size standard deviation of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly. Duplicated reads and clipped bases are included. No minimum mapping quality is imposed. - - Implementation details: In the NPM-sample-QC reference implementation it is computed using samtools stats, reporting the insert_size_standard_deviation field. Duplicated reads are included. No mapping qualiy is applied. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_samtools_stats"][biosample_id] - v = d["insert_size_standard_deviation"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def cross_contamination_rate(mqc, biosample_id): - """ - Description: Estimation of inter-sample contamination rate of short paired-end sequencing high quality, non duplicated reads, primary alignments, mapped on GRCh38 assembly. No minimum mapping quality is imposed. It is critical that the (BAM/CRAM) alignment files be readily marked for duplicated reads and clipped bases. - - Implementation details: The estimation of inter-sample DNA contamination of short paired-end sequencing high quality, aligned sequence reads (BAM/CRAM) mapped on GRCh38 assembly with pre-calculated reference panel of 1000 Genome Project dataset from the VerifyBamID resource using VerifyBamID2 with NumPC “4” (# of Principal Components used in estimation), the key information “FREEMIX” in “.selfSM” in the results indicates the estimated contamination level. - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_verifybamid"][biosample_id] - v = d["FREEMIX"] - except KeyError: - v = "NA" - - return k, v - -def count_snvs(mqc, biosample_id): - """ - The number of PASS SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pass_het_snps(mqc, biosample_id): - """ - The number of PASS het SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_het_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def pass_homalt_snps(mqc, biosample_id): - """ - The number of PASS hom alt SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_homalt_snps"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def ratio_heterozygous_homzygous_snv(mqc, biosample_id): - """ - The het/hom ratio for PASS SNPs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snp_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def ratio_heterozygous_homzygous_indel(mqc, biosample_id): - """ - The het/hom ratio for PASS INDELs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_indel_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def ratio_heterozygous_homzygous_insertion_deletion(mqc, biosample_id): - """ - The het/hom ratio for PASS insertions and deletions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins_del_het_hom"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - -def count_deletions(mqc, biosample_id): - """ - The number of PASS deletions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_del"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def count_insertions(mqc, biosample_id): - """ - The number of PASS insertions. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins"] - v = int(v) - except KeyError: - v = "NA" - - return k, v - -def ratio_insertion_deletion(mqc, biosample_id): - """ - The ins/del ratio for PASS INDELs. - - Source: count_variants.py (bcftools view) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_ins_del"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v - - -def ratio_transitions_transversions(mqc, biosample_id): - """ - The transition to transversion ratio of passing bi-allelic SNPs. - - Source: count_variants.py (bcftools stats - tstv) - """ - k = inspect.currentframe().f_code.co_name - - try: - d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] - v = d["pass_snp_ts_tv"] - v = np.round(v, DECIMALS) - except KeyError: - v = "NA" - - return k, v diff --git a/containers/Dockerfile.BAK b/containers/Dockerfile.BAK deleted file mode 100644 index 565121c9..00000000 --- a/containers/Dockerfile.BAK +++ /dev/null @@ -1,75 +0,0 @@ -FROM ubuntu:22.04 - -LABEL base_image="ubuntu:22.04" - -ENV LC_ALL=C.UTF-8 -ENV LANG=C.UTF-8 - -ENV PATH="/usr/local/conda/envs/npm-sample-qc/bin/:${PATH}" -ENV PATH="/usr/local/conda/envs/npm-sample-qc/share/picard-2.27.0-0/:${PATH}" -ENV PATH="/usr/local/conda/envs/npm-sample-qc/share/verifybamid2-2.0.1-7/:${PATH}" -ENV PATH="/usr/local/conda/bin/:${PATH}" - -ADD conda_env.yml /conda_env.yml - -RUN export DEBIAN_FRONTEND="noninteractive" && \ - apt-get update && \ - apt-get install -y --no-install-recommends \ - build-essential \ - libtool \ - libarchive-dev \ - make cmake m4 automake \ - gcc g++ \ - python3 python-pip python-dev-is-python3 \ - git \ - git-lfs \ - wget \ - zlib1g-dev \ - libbz2-dev \ - liblzma-dev \ - libmath-libm-perl \ - libpthread-stubs0-dev \ - curl \ - python3-pycurl \ - libncurses-dev \ - libboost-all-dev \ - libgtest-dev \ - cmake \ - libssl-dev \ - libcurl3-dev \ - unzip \ - gnuplot \ - datamash \ - graphviz \ - && rm -rf /var/lib/apt/lists/* - -RUN wget --max-redirect=1 https://github.com/samtools/bcftools/releases/download/1.19/bcftools-1.19.tar.bz2 \ - && tar -xf bcftools-1.19.tar.bz2 \ - && mkdir bcftools \ - && cd bcftools-1.19 \ - && ./configure --prefix=/bcftools \ - && make \ - && make install \ - && cd ../ \ - && rm bcftools-1.19.tar.bz2 \ - && apt-get install -y tabix - -ENV PATH="$PATH:/bcftools/bin" - - # install conda -RUN wget https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh -RUN bash Miniconda3-latest-Linux-x86_64.sh -b -p /usr/local/conda -RUN PATH="/usr/local/conda/bin:${PATH}" -RUN rm Miniconda3-latest-Linux-x86_64.sh -RUN conda update -n base -c defaults conda - -RUN conda install -c conda-forge -y awscli - - # create conda environment from template -RUN conda env create -f /conda_env.yml -RUN conda clean -a - - # install MultiQC and MultiQC_NPM plugin -RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git -WORKDIR /NPM-sample-qc/bin/multiqc_plugins -RUN python3 setup.py install From f3b902fb331e2bfb2101b7f7ca6ac4d3c816035b Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 15 Sep 2024 02:28:57 +0000 Subject: [PATCH 107/132] rename params aln/vcf file names --- .../{params.yaml.aln => params.aln.yaml} | 0 .../{params.yaml.vcf => params.vcf.yaml} | 0 .../{params.yaml.aln => params.aln.yaml} | 0 .../{params.yaml.vcf => params.vcf.yaml} | 0 4 files changed, 0 insertions(+), 0 deletions(-) rename tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/{params.yaml.aln => params.aln.yaml} (100%) rename tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/{params.yaml.vcf => params.vcf.yaml} (100%) rename tests/NA12878_1000genomes-dragen-3.7.6/{params.yaml.aln => params.aln.yaml} (100%) rename tests/NA12878_1000genomes-dragen-3.7.6/{params.yaml.vcf => params.vcf.yaml} (100%) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.aln.yaml similarity index 100% rename from tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.aln rename to tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.aln.yaml diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.vcf.yaml similarity index 100% rename from tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.yaml.vcf rename to tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/params.vcf.yaml diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln b/tests/NA12878_1000genomes-dragen-3.7.6/params.aln.yaml similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.aln rename to tests/NA12878_1000genomes-dragen-3.7.6/params.aln.yaml diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf b/tests/NA12878_1000genomes-dragen-3.7.6/params.vcf.yaml similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/params.yaml.vcf rename to tests/NA12878_1000genomes-dragen-3.7.6/params.vcf.yaml From f6eca2a133809ab7a43ca3ea7041dee96da240a8 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 15 Sep 2024 03:14:19 +0000 Subject: [PATCH 108/132] update metrics json output format --- bin/count_aln.py | 3 ++- bin/count_variants.py | 3 ++- 2 files changed, 4 insertions(+), 2 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index b07555f1..b0b37387 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -51,7 +51,8 @@ def raw_data(input_metrics): def save_output(data_metrics, outfile): with open(outfile, "w") as f: - data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : data_metrics} + # data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : data_metrics} + data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : {"aln_metrics" : data_metrics}} json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") diff --git a/bin/count_variants.py b/bin/count_variants.py index bc4cd6fc..8a39c4bf 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -226,7 +226,8 @@ def count_variants(input_vcf, scratch_dir, regions): def save_output(data_metrics, outfile): with open(outfile, "w") as f: # json.dump(data_metrics, f, sort_keys=True, indent=4) - data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} + #data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} + data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : {"variant_metrics" : data_metrics}} json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") From c044cccf5ee4b66bea3b59617eea0023e3c72659 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Sun, 15 Sep 2024 03:15:59 +0000 Subject: [PATCH 109/132] update metrics json output format --- .../NA12878-chr14-AKT1.aln.metrics.json | 18 +++++----- .../metrics/NA12878-chr14-AKT1.metrics.json | 34 +++++++++++-------- .../NA12878-chr14-AKT1.variant.metrics.json | 16 +++++---- 3 files changed, 38 insertions(+), 30 deletions(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json index 9d09d556..97362466 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json @@ -3,13 +3,15 @@ "id": "NA12878-chr14-AKT1" }, "wgs_qc_metrics": { - "insert_size_std_deviation": "98.2", - "mad_autosome_coverage": "0", - "mean_autosome_coverage": "0.000644", - "mean_insert_size": "447.3", - "pct_autosomes_15x": "0.000016", - "pct_reads_mapped": "99.63", - "pct_reads_properly_paired": "97.9", - "yield_bp_q30": "1996315" + "aln_metrics": { + "insert_size_std_deviation": "98.2", + "mad_autosome_coverage": "0", + "mean_autosome_coverage": "0.00", + "mean_insert_size": "447.3", + "pct_autosomes_15x": "0.00", + "pct_reads_mapped": "99.63", + "pct_reads_properly_paired": "97.9", + "yield_bp_q30": "1996315" + } } } diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json index ee117097..2f3693e0 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json @@ -3,20 +3,24 @@ "id": "NA12878-chr14-AKT1" }, "wgs_qc_metrics": { - "count_deletions": 848, - "count_insertions": 850, - "count_snvs": 8489, - "insert_size_std_deviation": "98.2", - "mad_autosome_coverage": "0", - "mean_autosome_coverage": "0.000644", - "mean_insert_size": "447.3", - "pct_autosomes_15x": "0.000016", - "pct_reads_mapped": "99.63", - "pct_reads_properly_paired": "97.9", - "ratio_heterozygous_homzygous_indel": 1.49, - "ratio_heterozygous_homzygous_snv": 1.05, - "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions_snv": 2.13, - "yield_bp_q30": "1996315" + "aln_metrics": { + "insert_size_std_deviation": "98.2", + "mad_autosome_coverage": "0", + "mean_autosome_coverage": "0.00", + "mean_insert_size": "447.3", + "pct_autosomes_15x": "0.00", + "pct_reads_mapped": "99.63", + "pct_reads_properly_paired": "97.9", + "yield_bp_q30": "1996315" + }, + "variant_metrics": { + "count_deletions": 848, + "count_insertions": 850, + "count_snvs": 8489, + "ratio_heterozygous_homzygous_indel": 1.49, + "ratio_heterozygous_homzygous_snv": 1.05, + "ratio_insertion_deletion": 1.0, + "ratio_transitions_transversions_snv": 2.13 + } } } diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json index 899b9a34..99ca6f45 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json @@ -3,12 +3,14 @@ "id": "NA12878-chr14-AKT1" }, "wgs_qc_metrics": { - "count_deletions": 848, - "count_insertions": 850, - "count_snvs": 8489, - "ratio_heterozygous_homzygous_indel": 1.49, - "ratio_heterozygous_homzygous_snv": 1.05, - "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions_snv": 2.13 + "variant_metrics": { + "count_deletions": 848, + "count_insertions": 850, + "count_snvs": 8489, + "ratio_heterozygous_homzygous_indel": 1.49, + "ratio_heterozygous_homzygous_snv": 1.05, + "ratio_insertion_deletion": 1.0, + "ratio_transitions_transversions_snv": 2.13 + } } } From 2e13a5d61c13ef889ffc86b164818a174d5bb1a4 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 17 Sep 2024 06:02:11 +0000 Subject: [PATCH 110/132] update metrics json format --- bin/compile_aln_variants_metrics.py | 3 ++- bin/count_aln.py | 2 +- bin/count_variants.py | 3 ++- modules/compile_aln_vcf/main.nf | 2 ++ 4 files changed, 7 insertions(+), 3 deletions(-) diff --git a/bin/compile_aln_variants_metrics.py b/bin/compile_aln_variants_metrics.py index 1c7734ed..0f6a74b2 100755 --- a/bin/compile_aln_variants_metrics.py +++ b/bin/compile_aln_variants_metrics.py @@ -48,7 +48,8 @@ def data2(input_variants_metrics): def save_output(data1, data2, outfile): with open(outfile, "w") as f: - json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) + json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) + #json.dump({"biosample": data1["biosample"], aln_input['wgs_qc_metrics']['variant_metric'].update(variants_input['wgs_qc_metrics']['variant_metric'])}, f, sort_keys=True, indent=4) f.write("\n") if __name__ == "__main__": diff --git a/bin/count_aln.py b/bin/count_aln.py index b0b37387..3401bd0a 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -52,7 +52,7 @@ def raw_data(input_metrics): def save_output(data_metrics, outfile): with open(outfile, "w") as f: # data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : data_metrics} - data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : {"aln_metrics" : data_metrics}} + data_metrics = {"biosample" : {"id" : args.sample_id}, "wgs_qc_metrics" : {"aln_metrics" : data_metrics, "variant_metrics" : {}}} json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") diff --git a/bin/count_variants.py b/bin/count_variants.py index 8a39c4bf..a39b8630 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -227,7 +227,8 @@ def save_output(data_metrics, outfile): with open(outfile, "w") as f: # json.dump(data_metrics, f, sort_keys=True, indent=4) #data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : data_metrics} - data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : {"variant_metrics" : data_metrics}} + #data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : {"aln_metrics" : {}, "variant_metrics" : data_metrics}} + data_metrics = {"biosample" : {"id" : args.biosample_id}, "wgs_qc_metrics" : {"variant_metrics" : data_metrics, "aln_metrics" : {}}} json.dump(data_metrics, f, sort_keys=True, indent=4) f.write("\n") diff --git a/modules/compile_aln_vcf/main.nf b/modules/compile_aln_vcf/main.nf index a87bd701..1b95246f 100644 --- a/modules/compile_aln_vcf/main.nf +++ b/modules/compile_aln_vcf/main.nf @@ -16,5 +16,7 @@ process compile_aln_vcf { --input_variants_metrics ${count_variants} \ --sample_id ${sample} \ --output_json ${sample}.metrics.json + + rm -rf ${count_aln} ${count_variants} """ } From b08d2e885fc776c0c05821882f42a1c1f6a685e2 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 04:43:48 +0000 Subject: [PATCH 111/132] update combining the aln and varaint metric json --- bin/compile_aln_variants_metrics.py | 7 +++++-- 1 file changed, 5 insertions(+), 2 deletions(-) diff --git a/bin/compile_aln_variants_metrics.py b/bin/compile_aln_variants_metrics.py index 0f6a74b2..322522d6 100755 --- a/bin/compile_aln_variants_metrics.py +++ b/bin/compile_aln_variants_metrics.py @@ -46,10 +46,13 @@ def data2(input_variants_metrics): variants_input = json.load(f2) return variants_input + def save_output(data1, data2, outfile): with open(outfile, "w") as f: - json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) - #json.dump({"biosample": data1["biosample"], aln_input['wgs_qc_metrics']['variant_metric'].update(variants_input['wgs_qc_metrics']['variant_metric'])}, f, sort_keys=True, indent=4) + data1['wgs_qc_metrics']['variant_metrics'].update(data2['wgs_qc_metrics']['variant_metrics']) + print(data1) + json.dump(data1, f, sort_keys=True, indent=4) + #json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) f.write("\n") if __name__ == "__main__": From 2305896ea007c2d7364de422c02bebdf446c8da5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 04:54:07 +0000 Subject: [PATCH 112/132] cleanup main.nf view --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 66bc8446..16b415ab 100644 --- a/main.nf +++ b/main.nf @@ -151,7 +151,7 @@ workflow { return tuple( rec.biosample_id ) } - .view() + //.view() .set { inputs_cond } @@ -234,7 +234,7 @@ workflow { .join( inputs_cond ) .join( count_aln.out.metrics ) .join( count_variants.out ) - .view() + //.view() .set { ch_count } compile_aln_vcf ( ch_count ) From d1f7aa2c2384046449550ffe3ea86dc91597ad54 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 05:09:38 +0000 Subject: [PATCH 113/132] update aln and varaint metric json --- .../results/metrics/NA12878-chr14-AKT1.aln.metrics.json | 3 ++- .../results/metrics/NA12878-chr14-AKT1.variant.metrics.json | 1 + 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json index 97362466..6c1994f8 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json @@ -12,6 +12,7 @@ "pct_reads_mapped": "99.63", "pct_reads_properly_paired": "97.9", "yield_bp_q30": "1996315" - } + }, + "variant_metrics": {} } } diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json index 99ca6f45..a05c8822 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json @@ -3,6 +3,7 @@ "id": "NA12878-chr14-AKT1" }, "wgs_qc_metrics": { + "aln_metrics": {}, "variant_metrics": { "count_deletions": 848, "count_insertions": 850, From c4d714843c93206d96617d2a253ae7be8bb94ecb Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 05:45:26 +0000 Subject: [PATCH 114/132] cleanup --- bin/compile_aln_variants_metrics.py | 1 + .../{NA12878.vc_metrics.csv => NA12878.vc_metrics.csv.BAK} | 0 2 files changed, 1 insertion(+) rename tests/NA12878_1000genomes-dragen-3.7.6/{NA12878.vc_metrics.csv => NA12878.vc_metrics.csv.BAK} (100%) diff --git a/bin/compile_aln_variants_metrics.py b/bin/compile_aln_variants_metrics.py index 322522d6..4a7f4a8a 100755 --- a/bin/compile_aln_variants_metrics.py +++ b/bin/compile_aln_variants_metrics.py @@ -50,6 +50,7 @@ def data2(input_variants_metrics): def save_output(data1, data2, outfile): with open(outfile, "w") as f: data1['wgs_qc_metrics']['variant_metrics'].update(data2['wgs_qc_metrics']['variant_metrics']) + #data1['wgs_qc_metrics']['variant_metrics'] = data2['wgs_qc_metrics']['variant_metrics'] print(data1) json.dump(data1, f, sort_keys=True, indent=4) #json.dump({"biosample": data1["biosample"], "wgs_qc_metrics": {**data1["wgs_qc_metrics"], **data2["wgs_qc_metrics"]}}, f, sort_keys=True, indent=4) diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv b/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK similarity index 100% rename from tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv rename to tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK From a222b3e01c16ec01c0f3d56ac9691f9dd6cfb8cc Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 05:46:27 +0000 Subject: [PATCH 115/132] cleanup --- .../NA12878.vc_metrics.csv.BAK | 25 ------------------- 1 file changed, 25 deletions(-) delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK b/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK deleted file mode 100644 index 32d02fcb..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/NA12878.vc_metrics.csv.BAK +++ /dev/null @@ -1,25 +0,0 @@ -VARIANT CALLER SUMMARY,,Number of samples,1 -VARIANT CALLER SUMMARY,,Reads Processed,649717949 -VARIANT CALLER SUMMARY,,Child Sample,NA -VARIANT CALLER POSTFILTER,NA12878,Total,5060888,100.00 -VARIANT CALLER POSTFILTER,NA12878,Biallelic,4869445,96.22 -VARIANT CALLER POSTFILTER,NA12878,Multiallelic,191443,3.78 -VARIANT CALLER POSTFILTER,NA12878,SNPs,4078055,80.58 -VARIANT CALLER POSTFILTER,NA12878,Insertions (Hom),167967,3.32 -VARIANT CALLER POSTFILTER,NA12878,Insertions (Het),308210,6.09 -VARIANT CALLER POSTFILTER,NA12878,Deletions (Hom),164236,3.25 -VARIANT CALLER POSTFILTER,NA12878,Deletions (Het),317555,6.27 -VARIANT CALLER POSTFILTER,NA12878,Indels (Het),24626,0.49 -VARIANT CALLER POSTFILTER,NA12878,Chr X number of SNPs over genome,127260 -VARIANT CALLER POSTFILTER,NA12878,Chr Y number of SNPs over genome,0 -VARIANT CALLER POSTFILTER,NA12878,(Chr X SNPs)/(chr Y SNPs) ratio over genome,NA -VARIANT CALLER POSTFILTER,NA12878,SNP Transitions,3740035 -VARIANT CALLER POSTFILTER,NA12878,SNP Transversions,1915655 -VARIANT CALLER POSTFILTER,NA12878,Ti/Tv ratio,1.95 -VARIANT CALLER POSTFILTER,NA12878,Heterozygous,3150535 -VARIANT CALLER POSTFILTER,NA12878,Homozygous,1910353 -VARIANT CALLER POSTFILTER,NA12878,Het/Hom ratio,1.65 -VARIANT CALLER POSTFILTER,NA12878,In dbSNP,0,0.00 -VARIANT CALLER POSTFILTER,NA12878,Not in dbSNP,0,0.00 -VARIANT CALLER POSTFILTER,NA12878,Percent Callability,97.2545 -VARIANT CALLER POSTFILTER,NA12878,Percent Autosome Callability,98.1391 From b14a5e231ae73c73634812d8f315921dbb79befa Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 06:10:58 +0000 Subject: [PATCH 116/132] assert int and float of metric value --- bin/count_aln.py | 14 ++++++++------ 1 file changed, 8 insertions(+), 6 deletions(-) diff --git a/bin/count_aln.py b/bin/count_aln.py index 3401bd0a..9ea00e5a 100755 --- a/bin/count_aln.py +++ b/bin/count_aln.py @@ -40,12 +40,14 @@ def raw_data(input_metrics): row = line.split('\t') key = row[0] value_str = row[1] - d[key] = value_str.replace("\n", "") - #try: - # value = float(value_str.strip()) - #except ValueError: - # value = value_str.strip() - #d[key] = value + #d[key] = value_str.replace("\n", "") + try: + #value = float(value_str.strip()) + value = int(value_str) + except ValueError: + #value = value_str.strip() + value = float(value_str.strip()) + d[key] = value return d From b200fbc755028177ed504ce498387ab10edfd651 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 18 Sep 2024 06:13:48 +0000 Subject: [PATCH 117/132] assert int and float of metric value --- .../metrics/NA12878-chr14-AKT1.aln.metrics.json | 16 ++++++++-------- .../metrics/NA12878-chr14-AKT1.metrics.json | 16 ++++++++-------- 2 files changed, 16 insertions(+), 16 deletions(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json index 6c1994f8..a3e89075 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json @@ -4,14 +4,14 @@ }, "wgs_qc_metrics": { "aln_metrics": { - "insert_size_std_deviation": "98.2", - "mad_autosome_coverage": "0", - "mean_autosome_coverage": "0.00", - "mean_insert_size": "447.3", - "pct_autosomes_15x": "0.00", - "pct_reads_mapped": "99.63", - "pct_reads_properly_paired": "97.9", - "yield_bp_q30": "1996315" + "insert_size_std_deviation": 98.2, + "mad_autosome_coverage": 0, + "mean_autosome_coverage": 0.0, + "mean_insert_size": 447.3, + "pct_autosomes_15x": 0.0, + "pct_reads_mapped": 99.63, + "pct_reads_properly_paired": 97.9, + "yield_bp_q30": 1996315 }, "variant_metrics": {} } diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json index 2f3693e0..82b1cddc 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json @@ -4,14 +4,14 @@ }, "wgs_qc_metrics": { "aln_metrics": { - "insert_size_std_deviation": "98.2", - "mad_autosome_coverage": "0", - "mean_autosome_coverage": "0.00", - "mean_insert_size": "447.3", - "pct_autosomes_15x": "0.00", - "pct_reads_mapped": "99.63", - "pct_reads_properly_paired": "97.9", - "yield_bp_q30": "1996315" + "insert_size_std_deviation": 98.2, + "mad_autosome_coverage": 0, + "mean_autosome_coverage": 0.0, + "mean_insert_size": 447.3, + "pct_autosomes_15x": 0.0, + "pct_reads_mapped": 99.63, + "pct_reads_properly_paired": 97.9, + "yield_bp_q30": 1996315 }, "variant_metrics": { "count_deletions": 848, From b06c79b052bc6124291c2f24811d3af00152b71d Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Thu, 19 Sep 2024 04:18:07 +0000 Subject: [PATCH 118/132] updated metric results --- .../results/metrics/NA12878.aln.metrics.json | 21 ++++++----- .../results/metrics/NA12878.metrics.json | 36 ++++++++++--------- .../metrics/NA12878.variant.metrics.json | 17 +++++---- 3 files changed, 42 insertions(+), 32 deletions(-) diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json index eb94cdbf..91d421c7 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json @@ -3,14 +3,17 @@ "id": "NA12878" }, "wgs_qc_metrics": { - "cross_contamination_rate": "1.8379e-06", - "insert_size_std_deviation": "98.7", - "mad_autosome_coverage": "4", - "mean_autosome_coverage": "31.080137", - "mean_insert_size": "439.8", - "pct_autosomes_15x": "0.959502", - "pct_reads_mapped": "99.76", - "pct_reads_properly_paired": "98.1", - "yield_bp_q30": "110066894077" + "aln_metrics": { + "cross_contamination_rate": 1.8379e-06, + "insert_size_std_deviation": 98.7, + "mad_autosome_coverage": 4, + "mean_autosome_coverage": 31.08, + "mean_insert_size": 439.8, + "pct_autosomes_15x": 95.95, + "pct_reads_mapped": 99.76, + "pct_reads_properly_paired": 98.1, + "yield_bp_q30": 110066894077 + }, + "variant_metrics": {} } } diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json index d1ac457c..c8132ab2 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.metrics.json @@ -3,21 +3,25 @@ "id": "NA12878" }, "wgs_qc_metrics": { - "count_deletions": 433328, - "count_insertions": 419222, - "count_snvs": 3904186, - "cross_contamination_rate": "1.8379e-06", - "insert_size_std_deviation": "98.7", - "mad_autosome_coverage": "4", - "mean_autosome_coverage": "31.080137", - "mean_insert_size": "439.8", - "pct_autosomes_15x": "0.959502", - "pct_reads_mapped": "99.76", - "pct_reads_properly_paired": "98.1", - "ratio_heterozygous_homzygous_indel": 1.96, - "ratio_heterozygous_homzygous_snv": 1.57, - "ratio_insertion_deletion": 0.97, - "ratio_transitions_transversions_snv": 1.96, - "yield_bp_q30": "110066894077" + "aln_metrics": { + "cross_contamination_rate": 1.8379e-06, + "insert_size_std_deviation": 98.7, + "mad_autosome_coverage": 4, + "mean_autosome_coverage": 31.08, + "mean_insert_size": 439.8, + "pct_autosomes_15x": 95.95, + "pct_reads_mapped": 99.76, + "pct_reads_properly_paired": 98.1, + "yield_bp_q30": 110066894077 + }, + "variant_metrics": { + "count_deletions": 433328, + "count_insertions": 419222, + "count_snvs": 3904186, + "ratio_heterozygous_homzygous_indel": 1.96, + "ratio_heterozygous_homzygous_snv": 1.57, + "ratio_insertion_deletion": 0.97, + "ratio_transitions_transversions_snv": 1.96 + } } } diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json index 26ec1554..9a9999cc 100644 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json +++ b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json @@ -3,12 +3,15 @@ "id": "NA12878" }, "wgs_qc_metrics": { - "count_deletions": 433328, - "count_insertions": 419222, - "count_snvs": 3904186, - "ratio_heterozygous_homzygous_indel": 1.96, - "ratio_heterozygous_homzygous_snv": 1.57, - "ratio_insertion_deletion": 0.97, - "ratio_transitions_transversions_snv": 1.96 + "aln_metrics": {}, + "variant_metrics": { + "count_deletions": 433328, + "count_insertions": 419222, + "count_snvs": 3904186, + "ratio_heterozygous_homzygous_indel": 1.96, + "ratio_heterozygous_homzygous_snv": 1.57, + "ratio_insertion_deletion": 0.97, + "ratio_transitions_transversions_snv": 1.96 + } } } From e6620bdfc240603cfe4b1a3f0386514da8faac63 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 23 Sep 2024 06:19:00 +0000 Subject: [PATCH 119/132] add count variants output emit name --- main.nf | 2 +- modules/count_variants/main.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 16b415ab..9ef48562 100644 --- a/main.nf +++ b/main.nf @@ -233,7 +233,7 @@ workflow { sample_ids .join( inputs_cond ) .join( count_aln.out.metrics ) - .join( count_variants.out ) + .join( count_variants.out.metrics ) //.view() .set { ch_count } diff --git a/modules/count_variants/main.nf b/modules/count_variants/main.nf index 7cf1ee4e..75d66d85 100644 --- a/modules/count_variants/main.nf +++ b/modules/count_variants/main.nf @@ -7,7 +7,7 @@ process count_variants { path(autosomes_non_gap_regions) output: - tuple val(sample), path("${sample}.variant.metrics.json") + tuple val(sample), path("${sample}.variant.metrics.json"), emit: metrics script: """ From 89b48310db877e984cf4641f1091979656965413 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 23 Sep 2024 06:39:46 +0000 Subject: [PATCH 120/132] removed redundant channels use of sampleids --- main.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 9ef48562..b8a2ea87 100644 --- a/main.nf +++ b/main.nf @@ -230,8 +230,9 @@ workflow { // channel for samplelist input file type aln and vcf processed outputs Channel .empty() - sample_ids - .join( inputs_cond ) + //sample_ids + //.join( inputs_cond ) + inputs_cond .join( count_aln.out.metrics ) .join( count_variants.out.metrics ) //.view() From 03fd678e11fa18f0526fb7c6cbf0d19ce665aa27 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 23 Sep 2024 07:59:08 +0000 Subject: [PATCH 121/132] include conditional input option to write unique metric output file --- main.nf | 80 +++++++++++++++++++++++++-------- modules/metric_aln/main.nf | 14 ++++++ modules/metric_variants/main.nf | 14 ++++++ nextflow.config | 28 ++++++++++-- 4 files changed, 114 insertions(+), 22 deletions(-) create mode 100644 modules/metric_aln/main.nf create mode 100644 modules/metric_variants/main.nf diff --git a/main.nf b/main.nf index b8a2ea87..d7ca1bdb 100644 --- a/main.nf +++ b/main.nf @@ -96,6 +96,8 @@ include { picard_collect_wgs_metrics as picard_collect_wgs_metrics_cram } from ' include { bcftools_stats } from './modules/bcftools' include { count_variants } from './modules/count_variants' include { count_aln } from './modules/count_aln' +include { metric_aln } from './modules/metric_aln' +include { metric_variants } from './modules/metric_variants' include { compile_aln_vcf } from './modules/compile_aln_vcf' /* @@ -124,6 +126,11 @@ workflow { .ifEmpty { ['biosample_id': params.biosample_id, 'aln': params.aln] } .set { samples } +// Channel to get sample id mapping + Channel + samples.map { it.biosample_id } + .set { sample_ids } + // Create channel branches for bam and cram input type Channel samples.branch { rec -> @@ -141,20 +148,6 @@ workflow { } .set { aln_inputs } -// Conditional input option to compile metrics if both input aln and vcf given - Channel - samples.branch { rec -> - def aln_file = rec.aln ? file( rec.aln ) : null - def vcf_file = rec.vcf ? file( rec.vcf ) : null - - count: rec.biosample_id && aln_file && vcf_file - - return tuple( rec.biosample_id ) - } - //.view() - .set { inputs_cond } - - samtools_stats_bam( aln_inputs.bam, [] ) samtools_stats_cram( aln_inputs.cram, ref_fasta ) @@ -183,10 +176,27 @@ workflow { //picard_collect_variant_calling_metrics_vcf( vcf_inputs, ref_dbsnp ) count_variants ( vcf_inputs, autosomes_non_gap_regions_bed ) -// Channel to get sample id mapping +// Conditional input option to rename metrics output file if vcf only given Channel - samples.map { it.biosample_id } - .set { sample_ids } + samples.branch { rec -> + def aln_file = rec.aln ? file( rec.aln ) : null + + count: rec.biosample_id && !aln_file + + return tuple( rec.biosample_id ) + } + .view() + .set { variants_cond } + + Channel + .empty() + variants_cond + .join( count_variants.out.metrics ) + .view() + .set { ch_variants_metric } + + metric_variants ( ch_variants_metric ) + /* // channel for samplelist vcf input file processed outputs @@ -226,8 +236,42 @@ workflow { count_aln ( aln_count_in ) +// Conditional input option to rename metrics output file if aln only given + Channel + samples.branch { rec -> + def vcf_file = rec.vcf ? file( rec.vcf ) : null + + count: rec.biosample_id && !vcf_file + + return tuple( rec.biosample_id ) + } + .view() + .set { aln_cond } + + Channel + .empty() + aln_cond + .join( count_aln.out.metrics ) + .view() + .set { ch_aln_metric } + + metric_aln ( ch_aln_metric ) + + +// Conditional input option to compile metrics if both input aln and vcf given + Channel + samples.branch { rec -> + def aln_file = rec.aln ? file( rec.aln ) : null + def vcf_file = rec.vcf ? file( rec.vcf ) : null + + count: rec.biosample_id && aln_file && vcf_file + + return tuple( rec.biosample_id ) + } + //.view() + .set { inputs_cond } -// channel for samplelist input file type aln and vcf processed outputs +// channel for samplelist input file type both aln and vcf processed outputs Channel .empty() //sample_ids diff --git a/modules/metric_aln/main.nf b/modules/metric_aln/main.nf new file mode 100644 index 00000000..da74d906 --- /dev/null +++ b/modules/metric_aln/main.nf @@ -0,0 +1,14 @@ +process metric_aln { + + tag { sample } + + input: + tuple val(sample), path(alncount) + + output: + tuple val(sample), path("${sample}.metrics.json"), emit: metrics + + """ + cp ${alncount} "${sample}.metrics.json" + """ +} diff --git a/modules/metric_variants/main.nf b/modules/metric_variants/main.nf new file mode 100644 index 00000000..0d19759f --- /dev/null +++ b/modules/metric_variants/main.nf @@ -0,0 +1,14 @@ +process metric_variants { + + tag { sample } + + input: + tuple val(sample), path(variantscount) + + output: + tuple val(sample), path("${sample}.metrics.json"), emit: metrics + + """ + cp ${variantscount} "${sample}.metrics.json" + """ +} diff --git a/nextflow.config b/nextflow.config index 2b00e4e8..1df8eedc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -107,6 +107,16 @@ process { disk = '50 GB' memory = '1 GB' +// publishDir = [ +// path: "${params.publishdir}/metrics", +// mode: params.publish_mode, +// ] + } + withName:metric_variants { + cpus = 1 + disk = '1 GB' + memory = '1 GB' + publishDir = [ path: "${params.publishdir}/metrics", mode: params.publish_mode, @@ -115,8 +125,18 @@ process { withName: 'count_aln' { cpus = 1 - disk = '50 GB' - memory = { 2.GB * task.attempt } + disk = '1 GB' + memory = { 1.GB * task.attempt } + +// publishDir = [ +// path: "${params.publishdir}/metrics", +// mode: params.publish_mode, +// ] + } + withName:metric_aln { + cpus = 1 + disk = '1 GB' + memory = '1 GB' publishDir = [ path: "${params.publishdir}/metrics", @@ -126,8 +146,8 @@ process { withName: 'compile_aln_vcf' { cpus = 1 - disk = '50 GB' - memory = { 2.GB * task.attempt } + disk = '1 GB' + memory = { 1.GB * task.attempt } publishDir = [ path: "${params.publishdir}/metrics", From f01d500105ba1f855780f54c7443547273db55a6 Mon Sep 17 00:00:00 2001 From: mhebrard Date: Tue, 24 Sep 2024 09:41:47 +0800 Subject: [PATCH 122/132] Delete aln & variant specific metrics.json from output_certified + run using nextflow + disable resume option --- .../NA12878-chr14-AKT1.aln.metrics.json | 18 ------------------ .../NA12878-chr14-AKT1.variant.metrics.json | 17 ----------------- .../run.sh | 3 +-- .../results/metrics/NA12878.aln.metrics.json | 19 ------------------- .../metrics/NA12878.variant.metrics.json | 17 ----------------- tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 3 +-- 6 files changed, 2 insertions(+), 75 deletions(-) delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json deleted file mode 100644 index a3e89075..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json +++ /dev/null @@ -1,18 +0,0 @@ -{ - "biosample": { - "id": "NA12878-chr14-AKT1" - }, - "wgs_qc_metrics": { - "aln_metrics": { - "insert_size_std_deviation": 98.2, - "mad_autosome_coverage": 0, - "mean_autosome_coverage": 0.0, - "mean_insert_size": 447.3, - "pct_autosomes_15x": 0.0, - "pct_reads_mapped": 99.63, - "pct_reads_properly_paired": 97.9, - "yield_bp_q30": 1996315 - }, - "variant_metrics": {} - } -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json deleted file mode 100644 index a05c8822..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json +++ /dev/null @@ -1,17 +0,0 @@ -{ - "biosample": { - "id": "NA12878-chr14-AKT1" - }, - "wgs_qc_metrics": { - "aln_metrics": {}, - "variant_metrics": { - "count_deletions": 848, - "count_insertions": 850, - "count_snvs": 8489, - "ratio_heterozygous_homzygous_indel": 1.49, - "ratio_heterozygous_homzygous_snv": 1.05, - "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions_snv": 2.13 - } - } -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh index e4085fa4..610fa8ff 100755 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh @@ -6,9 +6,8 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ +nextflow run ../../main.nf \ -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config \ --inputs_list params.yaml \ -work-dir ./work \ - -resume \ --publish_dir ./output diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json deleted file mode 100644 index 91d421c7..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json +++ /dev/null @@ -1,19 +0,0 @@ -{ - "biosample": { - "id": "NA12878" - }, - "wgs_qc_metrics": { - "aln_metrics": { - "cross_contamination_rate": 1.8379e-06, - "insert_size_std_deviation": 98.7, - "mad_autosome_coverage": 4, - "mean_autosome_coverage": 31.08, - "mean_insert_size": 439.8, - "pct_autosomes_15x": 95.95, - "pct_reads_mapped": 99.76, - "pct_reads_properly_paired": 98.1, - "yield_bp_q30": 110066894077 - }, - "variant_metrics": {} - } -} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json deleted file mode 100644 index 9a9999cc..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json +++ /dev/null @@ -1,17 +0,0 @@ -{ - "biosample": { - "id": "NA12878" - }, - "wgs_qc_metrics": { - "aln_metrics": {}, - "variant_metrics": { - "count_deletions": 433328, - "count_insertions": 419222, - "count_snvs": 3904186, - "ratio_heterozygous_homzygous_indel": 1.96, - "ratio_heterozygous_homzygous_snv": 1.57, - "ratio_insertion_deletion": 0.97, - "ratio_transitions_transversions_snv": 1.96 - } - } -} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 00cddcff..7ab59f8a 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -6,9 +6,8 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ +nextflow run ../../main.nf \ -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ - -resume \ --publish_dir ./output From 89cd4ad972e31a1db9b01b043815e87fa21948ed Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 24 Sep 2024 02:15:38 +0000 Subject: [PATCH 123/132] remove absolute path and resume arg --- tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh | 3 +-- tests/NA12878_1000genomes-dragen-3.7.6/run.sh | 3 +-- 2 files changed, 2 insertions(+), 4 deletions(-) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh index e4085fa4..610fa8ff 100755 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/run.sh @@ -6,9 +6,8 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ +nextflow run ../../main.nf \ -config ../../nextflow.config -config ./NA12878-chr14-AKT1.config \ --inputs_list params.yaml \ -work-dir ./work \ - -resume \ --publish_dir ./output diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh index 00cddcff..7ab59f8a 100755 --- a/tests/NA12878_1000genomes-dragen-3.7.6/run.sh +++ b/tests/NA12878_1000genomes-dragen-3.7.6/run.sh @@ -6,9 +6,8 @@ export CAPSULE_LOG=none NXF_ANSI_LOG=false -/home/ubuntu/bin/nf24.04.2/nextflow run ../../main.nf \ +nextflow run ../../main.nf \ -config ../../nextflow.config \ --inputs_list params.yaml \ -work-dir ./work \ - -resume \ --publish_dir ./output From 91e8f79fe2abae7f111a14ee1f67f870f2616348 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 24 Sep 2024 02:19:37 +0000 Subject: [PATCH 124/132] removed aln and variant only metrics json --- .../results/metrics/NA12878.aln.metrics.json | 19 ------------------- .../metrics/NA12878.variant.metrics.json | 17 ----------------- 2 files changed, 36 deletions(-) delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json delete mode 100644 tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json deleted file mode 100644 index 91d421c7..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.aln.metrics.json +++ /dev/null @@ -1,19 +0,0 @@ -{ - "biosample": { - "id": "NA12878" - }, - "wgs_qc_metrics": { - "aln_metrics": { - "cross_contamination_rate": 1.8379e-06, - "insert_size_std_deviation": 98.7, - "mad_autosome_coverage": 4, - "mean_autosome_coverage": 31.08, - "mean_insert_size": 439.8, - "pct_autosomes_15x": 95.95, - "pct_reads_mapped": 99.76, - "pct_reads_properly_paired": 98.1, - "yield_bp_q30": 110066894077 - }, - "variant_metrics": {} - } -} diff --git a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json b/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json deleted file mode 100644 index 9a9999cc..00000000 --- a/tests/NA12878_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878.variant.metrics.json +++ /dev/null @@ -1,17 +0,0 @@ -{ - "biosample": { - "id": "NA12878" - }, - "wgs_qc_metrics": { - "aln_metrics": {}, - "variant_metrics": { - "count_deletions": 433328, - "count_insertions": 419222, - "count_snvs": 3904186, - "ratio_heterozygous_homzygous_indel": 1.96, - "ratio_heterozygous_homzygous_snv": 1.57, - "ratio_insertion_deletion": 0.97, - "ratio_transitions_transversions_snv": 1.96 - } - } -} From f2b75f6135d68f804e82e3618879078c91b463c5 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Tue, 24 Sep 2024 02:21:30 +0000 Subject: [PATCH 125/132] removed aln and variant only metrics json --- .../NA12878-chr14-AKT1.aln.metrics.json | 18 ------------------ .../NA12878-chr14-AKT1.variant.metrics.json | 17 ----------------- 2 files changed, 35 deletions(-) delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json delete mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json deleted file mode 100644 index a3e89075..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.aln.metrics.json +++ /dev/null @@ -1,18 +0,0 @@ -{ - "biosample": { - "id": "NA12878-chr14-AKT1" - }, - "wgs_qc_metrics": { - "aln_metrics": { - "insert_size_std_deviation": 98.2, - "mad_autosome_coverage": 0, - "mean_autosome_coverage": 0.0, - "mean_insert_size": 447.3, - "pct_autosomes_15x": 0.0, - "pct_reads_mapped": 99.63, - "pct_reads_properly_paired": 97.9, - "yield_bp_q30": 1996315 - }, - "variant_metrics": {} - } -} diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json deleted file mode 100644 index a05c8822..00000000 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/output_certified/results/metrics/NA12878-chr14-AKT1.variant.metrics.json +++ /dev/null @@ -1,17 +0,0 @@ -{ - "biosample": { - "id": "NA12878-chr14-AKT1" - }, - "wgs_qc_metrics": { - "aln_metrics": {}, - "variant_metrics": { - "count_deletions": 848, - "count_insertions": 850, - "count_snvs": 8489, - "ratio_heterozygous_homzygous_indel": 1.49, - "ratio_heterozygous_homzygous_snv": 1.05, - "ratio_insertion_deletion": 1.0, - "ratio_transitions_transversions_snv": 2.13 - } - } -} From f5a5e1b1cbe6a468ce49c441660832967df63922 Mon Sep 17 00:00:00 2001 From: mhebrard Date: Tue, 24 Sep 2024 10:45:55 +0800 Subject: [PATCH 126/132] Reduce CPU requirement for count_variants + fix spaces --- nextflow.config | 31 ++++++++----------------------- 1 file changed, 8 insertions(+), 23 deletions(-) diff --git a/nextflow.config b/nextflow.config index 1df8eedc..31019277 100644 --- a/nextflow.config +++ b/nextflow.config @@ -58,6 +58,7 @@ process { mode: params.publish_mode, ] } + withName: 'picard_collect_multiple_metrics' { cpus = 1 @@ -69,6 +70,7 @@ process { mode: params.publish_mode, ] } + withName: 'picard_collect_wgs_metrics' { cpus = 1 @@ -80,6 +82,7 @@ process { mode: params.publish_mode, ] } + withName:bcftools_stats { cpus = 4 @@ -91,27 +94,13 @@ process { mode: params.publish_mode, ] } -// withName:picard_collect_variant_calling_metrics_vcf { -// -// cpus = 4 -// disk = '50 GB' -// memory = '2 GB' -// -// publishDir = [ -// path: "${params.publishdir}/CollectVariantCallingMetrics", -// mode: params.publish_mode, -// ] -// } + withName:count_variants { - cpus = 16 + cpus = 4 disk = '50 GB' memory = '1 GB' - -// publishDir = [ -// path: "${params.publishdir}/metrics", -// mode: params.publish_mode, -// ] } + withName:metric_variants { cpus = 1 disk = '1 GB' @@ -127,12 +116,8 @@ process { cpus = 1 disk = '1 GB' memory = { 1.GB * task.attempt } - -// publishDir = [ -// path: "${params.publishdir}/metrics", -// mode: params.publish_mode, -// ] } + withName:metric_aln { cpus = 1 disk = '1 GB' @@ -143,6 +128,7 @@ process { mode: params.publish_mode, ] } + withName: 'compile_aln_vcf' { cpus = 1 @@ -176,7 +162,6 @@ process.container = "cbig/npm-sample-qc:v${docker_version}" docker.enabled = true docker.runOptions = '-u \$(id -u):\$(id -g)' - docker.tmp = "auto" // To use container provisioning service integrated with Nextflow From 8723320804246328e6be6a36db87a18002a56c76 Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 13:26:04 +0800 Subject: [PATCH 127/132] delete out-of-place bed file --- autosomes_non_gap_regions.bed | 485 ---------------------------------- 1 file changed, 485 deletions(-) delete mode 100755 autosomes_non_gap_regions.bed diff --git a/autosomes_non_gap_regions.bed b/autosomes_non_gap_regions.bed deleted file mode 100755 index 660b7668..00000000 --- a/autosomes_non_gap_regions.bed +++ /dev/null @@ -1,485 +0,0 @@ -chr1 10000 207666 -chr1 257666 297968 -chr1 347968 535988 -chr1 585988 2702781 -chr1 2746290 12954384 -chr1 13004384 16799163 -chr1 16849163 29552233 -chr1 29553835 121976459 -chr1 122026459 122224535 -chr1 122224635 122503147 -chr1 122503247 124785432 -chr1 124785532 124849129 -chr1 124849229 124932724 -chr1 124932824 124977944 -chr1 124978326 125013060 -chr1 125013223 125026048 -chr1 125026071 125029104 -chr1 125029169 125103213 -chr1 125103233 125130246 -chr1 125131847 125171347 -chr1 125173583 125184587 -chr1 143184587 223558935 -chr1 223608935 228558364 -chr1 228608364 248946422 -chr2 10000 16145119 -chr2 16146119 89330679 -chr2 89530679 89685992 -chr2 89753992 90402511 -chr2 91402511 92138145 -chr2 92188145 94090557 -chr2 94140557 94293015 -chr2 94496015 97439618 -chr2 97489618 238903659 -chr2 238904047 242183529 -chr3 10000 90550102 -chr3 90550202 90565295 -chr3 90568828 90699772 -chr3 90699792 90722458 -chr3 90772458 91233586 -chr3 91233686 91247622 -chr3 91247722 91249905 -chr3 91256421 91257890 -chr3 91260180 91265381 -chr3 91276994 91282175 -chr3 91282734 91291069 -chr3 91291218 91345078 -chr3 91345173 91364131 -chr3 91364151 91438798 -chr3 91438818 91553319 -chr3 91553419 93655574 -chr3 93705574 198235559 -chr4 10000 1429358 -chr4 1434206 1435794 -chr4 1441552 8797477 -chr4 8816477 9272916 -chr4 9322916 31819295 -chr4 31832569 31835775 -chr4 31835795 32833016 -chr4 32839016 49336924 -chr4 49486924 49658100 -chr4 49708100 49711961 -chr4 49712061 51743951 -chr4 51793951 58878793 -chr4 58921381 190123121 -chr4 190173121 190204555 -chr5 10000 17530548 -chr5 17580548 46435900 -chr5 46485900 46569062 -chr5 46569162 46796725 -chr5 46796825 47061288 -chr5 47061388 47069162 -chr5 47073730 47078603 -chr5 47078720 47079733 -chr5 47082080 47106894 -chr5 47106994 47153339 -chr5 47153439 47296069 -chr5 47296169 47300585 -chr5 47306086 47309084 -chr5 47309184 49591369 -chr5 49591469 49592920 -chr5 49599323 49600986 -chr5 49601086 49603131 -chr5 49609714 49611721 -chr5 49612096 49618994 -chr5 49621605 49628165 -chr5 49630729 49633793 -chr5 49641815 49646823 -chr5 49646923 49650573 -chr5 49656261 49661871 -chr5 49666173 49667431 -chr5 49667531 49721203 -chr5 49721303 50059807 -chr5 50109807 139452659 -chr5 139453659 155760324 -chr5 155761324 181478259 -chr6 60000 58453888 -chr6 58553888 59829934 -chr6 60229934 61357029 -chr6 61363066 61370554 -chr6 61371372 61378482 -chr6 61378582 61381502 -chr6 61381602 61393846 -chr6 61393946 61398161 -chr6 61398261 95020790 -chr6 95070790 167591393 -chr6 167641393 170745979 -chr7 10000 237846 -chr7 240242 58119653 -chr7 58169653 60828234 -chr7 60878234 61063378 -chr7 61063426 61327788 -chr7 61377788 61528020 -chr7 61578020 61964169 -chr7 61967063 61976104 -chr7 62026104 62456779 -chr7 62506779 143650804 -chr7 143700804 159335973 -chr8 60000 7617127 -chr8 7667127 12234345 -chr8 12284345 43983744 -chr8 44033744 45877265 -chr8 45927265 85664222 -chr8 85714222 145078636 -chr9 10000 40529470 -chr9 40529480 40537052 -chr9 40537134 40547487 -chr9 40547497 40561938 -chr9 40561948 41225986 -chr9 41229378 41237752 -chr9 41238504 43222167 -chr9 43236167 43240559 -chr9 43240579 43254332 -chr9 43254352 43263290 -chr9 43263820 43268730 -chr9 43268750 43270944 -chr9 43274935 43276248 -chr9 43276457 43281323 -chr9 43282956 43332174 -chr9 43333269 43370405 -chr9 43371325 43377453 -chr9 43382279 43389535 -chr9 43389635 45518558 -chr9 60518558 60688432 -chr9 60738432 60779521 -chr9 60829521 61003887 -chr9 61053887 61231966 -chr9 61281966 61468808 -chr9 61518808 61735368 -chr9 61785368 62149738 -chr9 62249738 62748832 -chr9 62798832 62958371 -chr9 63008371 63202862 -chr9 63252862 63492264 -chr9 63542264 63918447 -chr9 63968447 64135013 -chr9 64185013 64215162 -chr9 64315162 64998124 -chr9 65048124 65080082 -chr9 65130082 65325123 -chr9 65375123 65595191 -chr9 65645191 66391387 -chr9 66591387 67920552 -chr9 68220552 134183092 -chr9 134185536 138334717 -chr10 10000 38529907 -chr10 38573338 38906036 -chr10 38911580 38913438 -chr10 38918269 39229918 -chr10 39230136 39238955 -chr10 39239118 39254773 -chr10 39254793 39338430 -chr10 39338450 39341685 -chr10 39341705 39409792 -chr10 39410237 39479351 -chr10 39479371 39497198 -chr10 39497296 39570652 -chr10 39570672 39585287 -chr10 39590435 39593013 -chr10 39597435 39598812 -chr10 39598832 39602699 -chr10 39606089 39607431 -chr10 39607451 39613189 -chr10 39615618 39617141 -chr10 39617255 39622353 -chr10 39625274 39635037 -chr10 39635057 39636682 -chr10 39686682 39935900 -chr10 39936000 41497440 -chr10 41497540 41545720 -chr10 41545820 41593521 -chr10 41693521 41916265 -chr10 42066265 47780368 -chr10 47870368 124121200 -chr10 124121502 131597030 -chr10 131597130 133690466 -chr10 133740466 133787422 -chr11 60000 50821348 -chr11 51078348 51090317 -chr11 51090417 54342399 -chr11 54342499 54425074 -chr11 54525074 70955696 -chr11 71055696 87978202 -chr11 88002896 96566178 -chr11 96566364 135076622 -chr12 10000 7083650 -chr12 7084650 34719407 -chr12 34769407 34816611 -chr12 34816711 34820185 -chr12 34820285 34822289 -chr12 34829237 34832088 -chr12 34832188 34835195 -chr12 34835295 37185252 -chr12 37235252 37240944 -chr12 37245716 37255332 -chr12 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12776204 12818137 -chr22 12868137 12904788 -chr22 12954788 12977325 -chr22 12977425 12986171 -chr22 12994027 13011653 -chr22 13014130 13021322 -chr22 13021422 13109444 -chr22 13109544 13163677 -chr22 13163777 13227312 -chr22 13227412 13248082 -chr22 13248182 13254852 -chr22 13254952 13258197 -chr22 13258297 13280858 -chr22 13280958 13285143 -chr22 13285243 14419454 -chr22 14419554 14419894 -chr22 14419994 14420334 -chr22 14420434 14421632 -chr22 14421732 15054318 -chr22 15154318 16279672 -chr22 16302843 16304296 -chr22 16305427 16307048 -chr22 16307605 16310302 -chr22 16310402 16313516 -chr22 16314010 18239129 -chr22 18339129 18433513 -chr22 18483513 18659564 -chr22 18709564 49973865 -chr22 49975365 50808468 From 408bfa11ea7fcb417a56fe63e5b30e5a943333bd Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 13:30:14 +0800 Subject: [PATCH 128/132] Clean Dockerfile --- containers/Dockerfile | 14 ++++---------- 1 file changed, 4 insertions(+), 10 deletions(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index 72610eb8..226956ac 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -44,17 +44,11 @@ RUN conda install -c conda-forge -y awscli RUN conda env create -f /conda_env.yml RUN conda clean -a -# install MultiQC and MultiQC_NPM plugin +# Clone master # RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git -RUN git clone -b feature/rework-aln-variant-metrics-json https://github.com/c-BIG/NPM-sample-qc.git -# RUN git clone https://github.com/c-BIG/NPM-sample-qc.git -# WORKDIR /NPM-sample-qc/bin/multiqc_plugins -WORKDIR /NPM-sample-qc/bin - -RUN git checkout feature/rework-aln-variant-metrics-json +# Clone develop +RUN git clone -b develop https://github.com/c-BIG/NPM-sample-qc.git -## RUN python3 setup.py install - -# RUN python -m pip install . +WORKDIR /NPM-sample-qc/bin RUN pip3 install numpy scipy argparse From 6a16fd96f32154d57f5d10e62e364ef23603266e Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 14:03:34 +0800 Subject: [PATCH 129/132] Remove justin local path from interval_list + Update nextflow requirement in readme --- README.rst | 4 +- .../autosomes_non_gap_regions.interval_list | 6732 ++++++++--------- .../chr14_non_gap_regions.interval_list | 2 +- 3 files changed, 3369 insertions(+), 3369 deletions(-) diff --git a/README.rst b/README.rst index f53919b3..31fec358 100644 --- a/README.rst +++ b/README.rst @@ -13,10 +13,10 @@ Requirements * `Install Nextflow`_ -v23.08.1-edge or higher :: +v23.10.4 or higher :: # Download the executable package - wget -qO- https://github.com/nextflow-io/nextflow/releases/download/v23.08.1-edge/nextflow-23.08.1-edge-all | bash + wget -qO- https://github.com/nextflow-io/nextflow/releases/download/v23.10.4/nextflow-23.10.4-all | bash # Make the binary executable on your system chmod -x nextflow # Optionally, move the nextflow file to a directory accessible by your $PATH variable diff --git 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UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8 LN:145138636 M5:c67955b5f7815a9a1edfaa15893d3616 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9 LN:138394717 M5:6c198acf68b5af7b9d676dfdd531b5de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr10 LN:133797422 M5:c0eeee7acfdaf31b770a509bdaa6e51a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11 LN:135086622 M5:1511375dc2dd1b633af8cf439ae90cec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12 LN:133275309 M5:96e414eace405d8c27a6d35ba19df56f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13 LN:114364328 M5:a5437debe2ef9c9ef8f3ea2874ae1d82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14 LN:107043718 M5:e0f0eecc3bcab6178c62b6211565c807 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15 LN:101991189 M5:f036bd11158407596ca6bf3581454706 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UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrY LN:57227415 M5:ce3e31103314a704255f3cd90369ecce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrM LN:16569 M5:c68f52674c9fb33aef52dcf399755519 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270706v1_random LN:175055 M5:62def1a794b3e18192863d187af956e6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270707v1_random LN:32032 M5:78135804eb15220565483b7cdd02f3be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270708v1_random LN:127682 M5:1e95e047b98ed92148dd84d6c037158c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270709v1_random LN:66860 M5:4e2db2933ea96aee8dab54af60ecb37d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270710v1_random LN:40176 M5:9949f776680c6214512ee738ac5da289 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270711v1_random LN:42210 M5:af383f98cf4492c1f1c4e750c26cbb40 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270712v1_random LN:176043 M5:c38a0fecae6a1838a405406f724d6838 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270713v1_random LN:40745 M5:cb78d48cc0adbc58822a1c6fe89e3569 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270714v1_random LN:41717 M5:42f7a452b8b769d051ad738ee9f00631 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270715v1_random LN:161471 M5:b65a8af1d7bbb7f3c77eea85423452bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270716v1_random LN:153799 M5:2828e63b8edc5e845bf48e75fbad2926 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_GL000221v1_random LN:155397 M5:3238fb74ea87ae857f9c7508d315babb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_GL000008v2_random LN:209709 M5:a999388c587908f80406444cebe80ba3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL000208v1_random LN:92689 M5:aa81be49bf3fe63a79bdc6a6f279abf6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_KI270717v1_random LN:40062 M5:796773a1ee67c988b4de887addbed9e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_KI270718v1_random LN:38054 M5:b0c463c8efa8d64442b48e936368dad5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_KI270719v1_random LN:176845 M5:cd5e932cfc4c74d05bb64e2126873a3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_KI270720v1_random LN:39050 M5:8c2683400a4aeeb40abff96652b9b127 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270721v1_random LN:100316 M5:9654b5d3f36845bb9d19a6dbd15d2f22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_GL000009v2_random LN:201709 M5:862f555045546733591ff7ab15bcecbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_GL000225v1_random LN:211173 M5:63945c3e6962f28ffd469719a747e73c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270722v1_random LN:194050 M5:51f46c9093929e6edc3b4dfd50d803fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_GL000194v1_random LN:191469 M5:6ac8f815bf8e845bb3031b73f812c012 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270723v1_random LN:38115 M5:74a4b480675592095fb0c577c515b5df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270724v1_random LN:39555 M5:c3fcb15dddf45f91ef7d94e2623ce13b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270725v1_random LN:172810 M5:edc6402e58396b90b8738a5e37bf773d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270726v1_random LN:43739 M5:fbe54a3197e2b469ccb2f4b161cfbe86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270727v1_random LN:448248 M5:84fe18a7bf03f3b7fc76cbac8eb583f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_KI270728v1_random LN:1872759 M5:369ff74cf36683b3066a2ca929d9c40d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL000205v2_random LN:185591 M5:458e71cd53dd1df4083dc7983a6c82c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270729v1_random LN:280839 M5:2756f6ee4f5780acce31e995443508b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270730v1_random LN:112551 M5:48f98ede8e28a06d241ab2e946c15e07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270731v1_random LN:150754 M5:8176d9a20401e8d9f01b7ca8b51d9c08 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270732v1_random LN:41543 M5:d837bab5e416450df6e1038ae6cd0817 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270733v1_random LN:179772 M5:f1fa05d48bb0c1f87237a28b66f0be0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270734v1_random LN:165050 M5:1d17410ae2569c758e6dd51616412d32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270735v1_random LN:42811 M5:eb6b07b73dd9a47252098ed3d9fb78b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270736v1_random LN:181920 M5:2ff189f33cfa52f321accddf648c5616 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270737v1_random LN:103838 M5:2ea8bc113a8193d1d700b584b2c5f42a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270738v1_random LN:99375 M5:854ec525c7b6a79e7268f515b6a9877c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270739v1_random LN:73985 M5:760fbd73515fedcc9f37737c4a722d6a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrY_KI270740v1_random LN:37240 M5:69e42252aead509bf56f1ea6fda91405 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270302v1 LN:2274 M5:ee6dff38036f7d03478c70717643196e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270304v1 LN:2165 M5:9423c1b46a48aa6331a77ab5c702ac9d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270303v1 LN:1942 M5:2cb746c78e0faa11e628603a4bc9bd58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270305v1 LN:1472 M5:f9acea3395b6992cf3418b6689b98cf9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270322v1 LN:21476 M5:7d459255d1c54369e3b64e719061a5a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270320v1 LN:4416 M5:d898b9c5a0118e76481bf5695272959e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270310v1 LN:1201 M5:af6cb123af7007793bac06485a2a20e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270316v1 LN:1444 M5:6adde7a9fe7bd6918f12d0f0924aa8ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270315v1 LN:2276 M5:ecc43e822dc011fae1fcfd9981c46e9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270312v1 LN:998 M5:26499f2fe4c65621fd8f4ecafbad31d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270311v1 LN:12399 M5:59594f9012d8ce21ed5d1119c051a2ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270317v1 LN:37690 M5:cd4b1fda800f6ec9ea8001994dbf6499 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270412v1 LN:1179 M5:7bb9612f733fb7f098be79499d46350c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270411v1 LN:2646 M5:fc240322d91d43c04f349cc58fda3eca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270414v1 LN:2489 M5:753e02ef3b1c590e0e3376ad2ebb5836 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270419v1 LN:1029 M5:58455e7a788f0dc82034d1fb109f6f5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270418v1 LN:2145 M5:1537ec12b9c58d137a2d4cb9db896bbc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270420v1 LN:2321 M5:bac954a897539c91982a7e3985a49910 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270424v1 LN:2140 M5:747c8f94f34d5de4ad289bc604708210 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270417v1 LN:2043 M5:cd26758fda713f9c96e51d541f50c2d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270422v1 LN:1445 M5:3fce80eb4c0554376b591699031feb56 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270423v1 LN:981 M5:bdf5a85c001731dccfb150db2bfe58ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270425v1 LN:1884 M5:665a46879bbb48294b0cfa87b61e71f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270429v1 LN:1361 M5:ee8962dbef9396884f649e566b78bf06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270442v1 LN:392061 M5:796289c4cda40e358991f9e672490015 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270466v1 LN:1233 M5:530b7033716a5d72dd544213c513fd12 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270465v1 LN:1774 M5:bb1b2323414425c46531b3c3d22ae00d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270467v1 LN:3920 M5:db34e0dc109a4afd499b5ec6aaae9754 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270435v1 LN:92983 M5:1655c75415b9c29e143a815f44286d05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270438v1 LN:112505 M5:e765271939b854dd6826aa764e930c87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270468v1 LN:4055 M5:0a603090f06108ed7aff75df0767b822 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270510v1 LN:2415 M5:cd7348b3b5d9d0dfef6aed2af75ce920 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270509v1 LN:2318 M5:1cdeb8c823d839e1d1735b5bc9a14856 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270518v1 LN:2186 M5:3fd898b62ca859f50fb8b83e7706352b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270508v1 LN:1951 M5:7d42a358d472b9cbdfdf30c8742473d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270516v1 LN:1300 M5:1cbaaafbbf016906a5bf5886f5a0ecb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270512v1 LN:22689 M5:ba1021c82d1230af856f59079e2f71b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270519v1 LN:138126 M5:8d754e9c9afd904fba0a2cd577fcc9a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270522v1 LN:5674 M5:070b4678e22501029c2e3297115216bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270511v1 LN:8127 M5:907ca34a4a2a6673632ebaf513a4c1a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270515v1 LN:6361 M5:dd7527ee8e0bdb0a43ca0b2a5456c8c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270507v1 LN:5353 M5:311894d0a815eb07c5cac49da851cb4a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270517v1 LN:3253 M5:913440c38d95c618617ca69bb9296170 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270529v1 LN:1899 M5:4caf890f2586daab8e4b2e2db904f05f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270528v1 LN:2983 M5:d75c9235f0b8c449fc4352997c56b086 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270530v1 LN:2168 M5:04549369e1197c626669a10164613635 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270539v1 LN:993 M5:19e3a982e67eafef39c5a3e4163f1e17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270538v1 LN:91309 M5:d60b72221cc7af871f2c757577e4c92a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270544v1 LN:1202 M5:e62a14b14467cdf48b5a236c66918f0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270548v1 LN:1599 M5:866b0db8e9cf66208c2c064bd09ce0a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270583v1 LN:1400 M5:b127e2e6dbe358ff192b271b8c6ee690 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270587v1 LN:2969 M5:36be47659719f47b95caa51744aa8f70 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270580v1 LN:1553 M5:1df30dae0f605811d927dcea58e729fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270581v1 LN:7046 M5:9f26945f9f9b3f865c9ebe953cbbc1a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270579v1 LN:31033 M5:fe62fb1964002717cc1b034630e89b1f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270589v1 LN:44474 M5:211c215414693fe0a2399cf82e707e03 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270590v1 LN:4685 M5:e8a57f147561b361091791b9010cd28b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270584v1 LN:4513 M5:d93636c9d54abd013cfc0d4c01334032 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270582v1 LN:6504 M5:6fd9804a7478d2e28160fe9f017689cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270588v1 LN:6158 M5:37ffa850e69b342a8f8979bd3ffc77d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270593v1 LN:3041 M5:f4a5bfa203e9e81acb640b18fb11e78e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270591v1 LN:5796 M5:d6af509d69835c9ac25a30086e5a4051 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270330v1 LN:1652 M5:c2c590706a339007b00c59e0b8937e78 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270329v1 LN:1040 M5:f023f927ae84c5cc48dc4dce11ba90f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270334v1 LN:1368 M5:53afe12d1371f250a3d1de655345d374 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270333v1 LN:2699 M5:57baf650c47bba9b3a8b7c6d0fb55ad6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270335v1 LN:1048 M5:eb27188639503b524d2659a23b8262ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270338v1 LN:1428 M5:301ef75a6b2996d745eb3464bd352b57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270340v1 LN:1428 M5:56b462bac20d385cdfcde0155fe4c3a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270336v1 LN:1026 M5:69ad2d85d870c8b0269434581e86e30e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270337v1 LN:1121 M5:16fc8d71a2662a6cfec7bdeec3d810c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270363v1 LN:1803 M5:6edd17a912f391022edbc192d49f2489 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270364v1 LN:2855 M5:6ff66a8e589ca27d93b5bac0e5b13a87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270362v1 LN:3530 M5:bc82401ffd9a5ae711fa0ea34da8d2f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270366v1 LN:8320 M5:44a0b65b7ba6bcff37eca202e7d966ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270378v1 LN:1048 M5:fc13bda7dbd914c92fb7e49489d1350f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270379v1 LN:1045 M5:3218bef25946cd95de585dfc7750f63b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270389v1 LN:1298 M5:2c9b08c57c27e714d4d5259fd91b6983 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270390v1 LN:2387 M5:7a64d89ea14990c16d20f4d6e7283e10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270387v1 LN:1537 M5:22a12462264340c25e912b8485cdfa91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270395v1 LN:1143 M5:7c03ca4756c1620f318fb189214388d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270396v1 LN:1880 M5:9069bed3c2efe7cc87227d619ad5816f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270388v1 LN:1216 M5:76f9f3315fa4b831e93c36cd88196480 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270394v1 LN:970 M5:d5171e863a3d8f832f0559235987b1e5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270386v1 LN:1788 M5:b9b1baaa7abf206f6b70cf31654172db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270391v1 LN:1484 M5:1fa5cf03b3eac0f1b4a64948fd09de53 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270383v1 LN:1750 M5:694d75683e4a9554bcc1291edbcaee43 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270393v1 LN:1308 M5:3724e1d70677d6b5c4bcf17fd40da111 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270384v1 LN:1658 M5:b06e44ea15d0a57618d6ca7d2e6ac5d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270392v1 LN:971 M5:59b3ca8de65fb171683f8a06d3b4bf0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270381v1 LN:1930 M5:2a9297cfd3b3807195ab9ad07e775d99 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270385v1 LN:990 M5:112a8b1df94ef0498a0bfe2d2ea5cc23 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270382v1 LN:4215 M5:e7085cdcee6ad62f359744e13d3209fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270376v1 LN:1136 M5:59e8fc80b78d62325082334b43dffdba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270374v1 LN:2656 M5:dbc92c9a92e558946e58b4909ec95dd5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270372v1 LN:1650 M5:53a9d5e8fd28bce5da5efcfd9114dbf2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270373v1 LN:1451 M5:b174fe53be245a840cd6324e39b88ced UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270375v1 LN:2378 M5:d678250c97e9b94aa390fa46e70a6d83 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270371v1 LN:2805 M5:a0af3d778dfeb7963e8e6d84c0c54fba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270448v1 LN:7992 M5:0f40827c265cb813b6e723da6c9b926b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270521v1 LN:7642 M5:af5bef7cefec7bd7efa729ac6c5be088 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000195v1 LN:182896 M5:5d9ec007868d517e73543b005ba48535 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000219v1 LN:179198 M5:f977edd13bac459cb2ed4a5457dba1b3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000220v1 LN:161802 M5:fc35de963c57bf7648429e6454f1c9db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000224v1 LN:179693 M5:d5b2fc04f6b41b212a4198a07f450e20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270741v1 LN:157432 M5:86eaea8a15a3950e37442eaaa5c9dc92 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000226v1 LN:15008 M5:1c1b2cd1fccbc0a99b6a447fa24d1504 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000213v1 LN:164239 M5:9d424fdcc98866650b58f004080a992a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270743v1 LN:210658 M5:3b62d9d3100f530d509e4efebd98502c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270744v1 LN:168472 M5:e90aee46b947ff8c32291a6843fde3f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270745v1 LN:41891 M5:1386fe3de6f82956f2124e19353ff9c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270746v1 LN:66486 M5:c470486a0a858e14aa21d7866f83cc17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270747v1 LN:198735 M5:62375d812ece679c9fd2f3d08d4e22a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270748v1 LN:93321 M5:4f6c6ab005c852a4352aa33e7cc88ded UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270749v1 LN:158759 M5:c899a7b4e911d371283f3f4058ca08b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270750v1 LN:148850 M5:c022ba92f244b7dc54ea90c4eef4d554 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270751v1 LN:150742 M5:1b758bbdee0e9ca882058d916cba9d29 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270752v1 LN:27745 M5:e0880631848337bd58559d9b1519da63 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270753v1 LN:62944 M5:25075fb2a1ecada67c0eb2f1fe0c7ec9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270754v1 LN:40191 M5:fe9e16233cecbc244f06f3acff3d03b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270755v1 LN:36723 M5:4a7da6a658955bd787af8add3ccb5751 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270756v1 LN:79590 M5:2996b120a5a5e15dab6555f0bf92e374 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270757v1 LN:71251 M5:174c73b60b41d8a1ef0fbaa4b3bdf0d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000214v1 LN:137718 M5:46c2032c37f2ed899eb41c0473319a69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KI270742v1 LN:186739 M5:2f31c013a4a8301deb8ab7ed1ca1cd99 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000216v2 LN:176608 M5:725009a7e3f5b78752b68afa922c090c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_GL000218v1 LN:161147 M5:1d708b54644c26c7e01c2dad5426d38c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270762v1_alt LN:354444 M5:b0397179e5a92bb7a3300b68e45a9f72 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270766v1_alt LN:256271 M5:e3f36479d0e07abbd0d47babdc76e19d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270760v1_alt LN:109528 M5:f2ea4b127c54df13f53a2b2b5a358087 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270765v1_alt LN:185285 M5:628b663499df4f5de7dbdd56943bdb6e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_GL383518v1_alt LN:182439 M5:978987018f1a910273ebcc387e038de8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_GL383519v1_alt LN:110268 M5:349e96f115f829409bd1087b5fb684ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_GL383520v2_alt LN:366580 M5:52e97087cc76c9fd73d4815c1e379ee0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270764v1_alt LN:50258 M5:36d9e8a04906213f8c1d4cf81fc4f83f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270763v1_alt LN:911658 M5:2f651e42b9fd434c2310be375fe8645e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270759v1_alt LN:425601 M5:8477794d60329af9bd00cd9277981a59 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270761v1_alt LN:165834 M5:3909a76846aa6ed534ba6be66a60cc81 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270770v1_alt LN:136240 M5:38181b04426519779c84cf9a8927cf00 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270773v1_alt LN:70887 M5:aa8b8ec2dd7776e643699db29ac85d74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270774v1_alt LN:223625 M5:a6d8be44258a4b8e3b55ffaccbd3b444 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270769v1_alt LN:120616 M5:537f266cfc0ba05dfb532658fb592eb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_GL383521v1_alt LN:143390 M5:8c6f0a214ddbfbd52be574a566e4b21a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270772v1_alt LN:133041 M5:7cc5209d7e796e90bd024204c04a9b7f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270775v1_alt LN:138019 M5:79c3c0ad56276d3eff8cb747b57973a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270771v1_alt LN:110395 M5:3739c7d552cfc62c495704f4f2d61330 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270768v1_alt LN:110099 M5:5f6d54708a2fd0e4592319c2c91850a7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_GL582966v2_alt LN:96131 M5:485c442c93fe19514153702f0c84d952 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_GL383522v1_alt LN:123821 M5:df3e809f9a87f792218db18db51f6ad4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270776v1_alt LN:174166 M5:87fe3336a33a4aa9c04ff7991aa78a11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270767v1_alt LN:161578 M5:160c7bb94aa35c1d61c27aadf1727862 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_JH636055v2_alt LN:173151 M5:42f48ed9d46aded55795ef64e4713d55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270783v1_alt LN:109187 M5:3a60aafd707904d4da9c1c090a7cf0ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270780v1_alt LN:224108 M5:2c050e6aeecf29b54469dea8c5631057 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_GL383526v1_alt LN:180671 M5:620913159e2fbd4e931ac120e3c584c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270777v1_alt LN:173649 M5:9673b29322cf16da2d295778229c1772 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270778v1_alt LN:248252 M5:f0053d7b401730af8c34cd7d20dd0a91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270781v1_alt LN:113034 M5:c94ff451868540e0399bf62acb24e1dd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270779v1_alt LN:205312 M5:3eec883e9656d4c4d96ea1e6d3c118ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270782v1_alt LN:162429 M5:ceaa7d4ca7d4b80ba9a8d9e48223de2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270784v1_alt LN:184404 M5:35980922a4773cefc710c08134d16772 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270790v1_alt LN:220246 M5:07cf7caef89c4677af575c370a1ebbb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_GL383528v1_alt LN:376187 M5:2948653361f974fbed3e26a4dfbf332c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270787v1_alt LN:111943 M5:e315d4f96ad548f1af1935d97f2af9df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_GL000257v2_alt LN:586476 M5:03c6c36060cd6c9f9b4fd0b096ff40e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270788v1_alt LN:158965 M5:463baeb6369579e0d387371989492dc0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_GL383527v1_alt LN:164536 M5:6d728406957c5c7fb158dbdb7efef2b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270785v1_alt LN:119912 M5:4932c3e044a1e3731cd4de290f6492b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270789v1_alt LN:205944 M5:3c9ef00afbe249de4934eeac37d64233 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270786v1_alt LN:244096 M5:107c6b17f17b5d24936663fe8e70cd2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270793v1_alt LN:126136 M5:0c6c632ce75031c86e3054c34d3b9b49 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270792v1_alt LN:179043 M5:3677eaca510b7e7c5f30ec8382a09d24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270791v1_alt LN:195710 M5:3a62433e49995583fc44e640241a60bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL383532v1_alt LN:82728 M5:eb61b6b3f9374b05ce68ae4a393cf5ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL949742v1_alt LN:226852 M5:20d5046bbd2a21729fdd64fa94bdd5a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270794v1_alt LN:164558 M5:9808234a2843375d92d4c34e262373b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL339449v2_alt LN:1612928 M5:1b6fa375fdf382778e6645d822d12254 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL383530v1_alt LN:101241 M5:adcb4048e465b6b949990853e436a136 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270796v1_alt LN:172708 M5:6b125232b8842fafd4179203620fa2be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_GL383531v1_alt LN:173459 M5:9161d10779b02fa44b841136ef9e499c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270795v1_alt LN:131892 M5:6670fafb8f6ceb4f46392f3178f29666 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000250v2_alt LN:4672374 M5:740358c10fbb870c86a7e44226bbdb3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270800v1_alt LN:175808 M5:3ed7e87ce250a8466359176e96d6a6a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270799v1_alt LN:152148 M5:80fd907ae0e4d20ad13960cb92ab6d45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL383533v1_alt LN:124736 M5:7d1a65603558094937299ff10e8714af UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270801v1_alt LN:870480 M5:bd0c66e2085cf16906ebc6b130862bd6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270802v1_alt LN:75005 M5:c66357c9b0909ee32c75286796602ce3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KB021644v2_alt LN:185823 M5:332d693d4f2026747d670e52836694ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270797v1_alt LN:197536 M5:2b76b3d07dc4cb2992e7125c19e32594 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270798v1_alt LN:271782 M5:7f074e537d58c543d116ce2c45bb0966 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270804v1_alt LN:157952 M5:9babd3431c32811769c782703f7b8c3d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270809v1_alt LN:209586 M5:26d14130d09dc093446dd31e8f0be76c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270806v1_alt LN:158166 M5:b55d6ad642408486735aede89d70c0c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_GL383534v2_alt LN:119183 M5:c6ff49147dedce02366d6ade10580611 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270803v1_alt LN:1111570 M5:986e63220694b8c91bad1d6d990f6c83 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270808v1_alt LN:271455 M5:52ce11809c32fe22003c5039e407d3e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270807v1_alt LN:126434 M5:4da184771ac6a8644e6df506bbd8a35e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270805v1_alt LN:209988 M5:d3a95141230a470a930f685fbce90a04 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270818v1_alt LN:145606 M5:5f8af0060f092d941cbe9d873ccc1d41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270812v1_alt LN:282736 M5:93b572cdabe748641570fa2ed3391222 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270811v1_alt LN:292436 M5:dacc0b0ef9fae890a1741dca03ba7d5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270821v1_alt LN:985506 M5:bbdee492e8852d313be96b6340fbfb1c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270813v1_alt LN:300230 M5:c91599edb1c553cf527c7f3ba754bb20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270822v1_alt LN:624492 M5:2cd6749ad4385747595c0b41440cb41d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270814v1_alt LN:141812 M5:84d9d4bbbac92a40b13809e501ff92fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270810v1_alt LN:374415 M5:369545bbf4f206fd1175a84da12b38fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270819v1_alt LN:133535 M5:29d6d49a19ae259feb012f492ab83ce0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270820v1_alt LN:36640 M5:d3b4609d5f29708fc33023259e4b775a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270817v1_alt LN:158983 M5:d6e6a3ac3c2f5afe16c2cfb2e813b30c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270816v1_alt LN:305841 M5:60bb5379ab4d817058300ef606b9fee6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270815v1_alt LN:132244 M5:8be72bf872ff1edfc3f43cd9b7d0b9b3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_GL383539v1_alt LN:162988 M5:12406aad3f3da31bda9c21a1aa0e16b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_GL383540v1_alt LN:71551 M5:23aea04f46682e2a2be1a5ff3934a9fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_GL383541v1_alt LN:171286 M5:0c787911df2449cbba8609bebf897ecb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_GL383542v1_alt LN:60032 M5:12a3180640a49f33c960eb12ca61a6c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr9_KI270823v1_alt LN:439082 M5:ad4baa1567e313eda0aad427008e10cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr10_GL383545v1_alt LN:179254 M5:c27dc6fea378fecf178a44682257c25e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr10_KI270824v1_alt LN:181496 M5:af40e32c32de290cfe497a110ff3bdfa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr10_GL383546v1_alt LN:309802 M5:ed6fb45e0a25c31903cbb0f78d9d487e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr10_KI270825v1_alt LN:188315 M5:3119d196965ee9cae7aeda77131076f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270832v1_alt LN:210133 M5:2b63a3f97e490e807587639807d26293 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270830v1_alt LN:177092 M5:dbe0c94a39d4a91469ffa6e509b3f191 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270831v1_alt LN:296895 M5:b0f755388212c03651b6fdc7531c3fd2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270829v1_alt LN:204059 M5:6bc1308e442a1662ae27952f126b7c01 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_GL383547v1_alt LN:154407 M5:7b556f03729e304a286c8d7ef0f0c10e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_JH159136v1_alt LN:200998 M5:8ac9fb9d942dba38bfd30f8d767f4bba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_JH159137v1_alt LN:191409 M5:b293c854ddcbc316cb1d449bca46fbb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270827v1_alt LN:67707 M5:86017dee76a03e3dc3c8833018101fae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270826v1_alt LN:186169 M5:a903d85efe5aa37dac36160bddeeac87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL877875v1_alt LN:167313 M5:3dd30a7638c3a3c518fc15571546b1be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL877876v1_alt LN:408271 M5:5c3a364520bf7ed46894abdce8f6e032 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270837v1_alt LN:40090 M5:e749a70a94624f09e134600d122c130d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL383549v1_alt LN:120804 M5:60a7c1711d7f23fd7311a7e4f96896f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270835v1_alt LN:238139 M5:b163c6fa013f6cb75b01088689f1a67f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL383550v2_alt LN:169178 M5:d61d9a153c3fd556b48e1c511a6145e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL383552v1_alt LN:138655 M5:c28f12c6ee0dec4cc6995766a710960c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL383553v2_alt LN:152874 M5:89b111e38005345de92036b249ab6080 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270834v1_alt LN:119498 M5:3ad1c94c00299533f6acd33ea2f04df1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_GL383551v1_alt LN:184319 M5:d96719c32333013a51c4d6d3261f984f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270833v1_alt LN:76061 M5:8c9988aa66d02708e3e57663902fc664 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270836v1_alt LN:56134 M5:28a69648439a2c02d46750eee929b20d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270840v1_alt LN:191684 M5:d90a653d7ac3171c8fb762d0012249f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270839v1_alt LN:180306 M5:b97ea581776626ac41b69f851a85a456 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270843v1_alt LN:103832 M5:563c8412eb284fc5321af590a4c9ab9d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270841v1_alt LN:169134 M5:6ac8a99077817e6bc9be36327b8aa18e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270838v1_alt LN:306913 M5:baf90eafb4ed6330cb151c1d5538f721 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr13_KI270842v1_alt LN:37287 M5:231dd4484df464129d3120986fe2af73 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270844v1_alt LN:322166 M5:b64acbff6218bd210676141e4e678a82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270847v1_alt LN:1511111 M5:9d380ae26e2edb178ef0121021f7a7ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270845v1_alt LN:180703 M5:51b3fa9f43d11aeb91b3debb33496d22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr14_KI270846v1_alt LN:1351393 M5:1ee28b84d30d13e03341b382995b05bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270852v1_alt LN:478999 M5:5104fe22193b4bd04166ecb401beb06a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270851v1_alt LN:263054 M5:64217994ed61c2feb5412ecc5a7a37b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270848v1_alt LN:327382 M5:d61e4262335c4884ad97dc5d73782870 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_GL383554v1_alt LN:296527 M5:4a3d54bda53308ca941d6d0e794b05cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270849v1_alt LN:244917 M5:7966604eef1b897ee97e8dce9e79b18e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_GL383555v2_alt LN:388773 M5:c904b1792869ae0565acbbdc19b9522f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270850v1_alt LN:430880 M5:5371d98deea30e91051e3efd20016b76 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_KI270854v1_alt LN:134193 M5:0e126949f4b012d27f55aed5f1c4ca84 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_KI270856v1_alt LN:63982 M5:515d2b9de600d584473c20bea079f34c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_KI270855v1_alt LN:232857 M5:436afa974458133c013b037d4a2dc1c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_KI270853v1_alt LN:2659700 M5:5e28daf08ffeafb037b6e97ba3c959e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_GL383556v1_alt LN:192462 M5:bed6a2667e8452a176e93e921e0c21f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr16_GL383557v1_alt LN:89672 M5:0989118882ce9c38635e2809e5bf71c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL383563v3_alt LN:375691 M5:fcd64ac83b2ebb57f50931c9d6fc0a31 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270862v1_alt LN:391357 M5:f2efc8dc887a8c0101053fa14491e07f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270861v1_alt LN:196688 M5:808750bf9b346e0a731a1bffac75a01e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270857v1_alt LN:2877074 M5:f5ca1bb91881efefa473c983f9519561 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_JH159146v1_alt LN:278131 M5:384b5b32f0ea2cfd15ac268a2ce07909 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_JH159147v1_alt LN:70345 M5:4bf63957bfa1ecdbbab483d4c0ce6682 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL383564v2_alt LN:133151 M5:fc2eb07543a6ecb0a27d9fa12cd91cfc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL000258v2_alt LN:1821992 M5:8bd6071029f2dbc5ab9d2d912bf5c953 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL383565v1_alt LN:223995 M5:063358c8e7f81361b959efab7b3f15cc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270858v1_alt LN:235827 M5:69fbdc82c5ef837f574d0b3c8b9c647f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270859v1_alt LN:108763 M5:8d28bd3b1a63fa9a945051f28e087668 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_GL383566v1_alt LN:90219 M5:a0f25165c6537c9861cc1231f710e99f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270860v1_alt LN:178921 M5:91e560df1ad6c58e7e1739178b8c7209 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_KI270864v1_alt LN:111737 M5:04e6ca7eabe96be35a18eb4c0675071d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383567v1_alt LN:289831 M5:d9015dd9a0916a98ed8ab99fd3cdd012 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383570v1_alt LN:164789 M5:08366c03855961f13b1d5e65920ccf74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383571v1_alt LN:198278 M5:40015159c7da8f06875bb558587e3f07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383568v1_alt LN:104552 M5:e9aba11d18125a2bce2b1e5915e9a904 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383569v1_alt LN:167950 M5:8d13c3e7cbb2b7e1a3225c5a54fe8f44 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_GL383572v1_alt LN:159547 M5:8fc7aaa775b43df3d77c9782a140a981 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_KI270863v1_alt LN:167999 M5:2e691a3fc5108cc7decde377ef398fde UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270868v1_alt LN:61734 M5:2dfd26bf8f217b858edc062be1846db5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270865v1_alt LN:52969 M5:b95ce15823c60ab83ec7a984f3dc9ebc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL383573v1_alt LN:385657 M5:5404455aab275489bc8e6c9fb3ead5cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL383575v2_alt LN:170222 M5:dd8730d9d33765ff135fcfadb8810280 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL383576v1_alt LN:188024 M5:8d4496a682182f8273a2fad665fcf4a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL383574v1_alt LN:155864 M5:0386df1d3476e6649f919195cc072fc7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270866v1_alt LN:43156 M5:d205b57d55e89546400e0f1e230a8a53 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270867v1_alt LN:233762 M5:3d8464a9e183031ea70e0e12fa231163 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949746v1_alt LN:987716 M5:d76e635e75bc038782fb3d0c195d33fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr20_GL383577v2_alt LN:128386 M5:f00c218d09df83c8b515a6b5591fe30c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr20_KI270869v1_alt LN:118774 M5:2b8065d3bdbac6664d7f35c25d049113 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr20_KI270871v1_alt LN:58661 M5:29e7c064fe54ab6b7fd8accb0363821b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr20_KI270870v1_alt LN:183433 M5:779f6fd5220eddc54631e80b94bb905a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_GL383578v2_alt LN:63917 M5:d512f47a48fc477e7232d3b46f2bda86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_KI270874v1_alt LN:166743 M5:45c435343bfd1d94c4ff17689faf507d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_KI270873v1_alt LN:143900 M5:22bc49cad0dda4ec9d79ffd619be94d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_GL383579v2_alt LN:201197 M5:631f76952802048256225746f1a14a89 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_GL383580v2_alt LN:74653 M5:b0cfe93a0987bda7488445b3c37b2516 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_GL383581v2_alt LN:116689 M5:1c9b893acb0fb6a6d9ff1915ce9004ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr21_KI270872v1_alt LN:82692 M5:e8daa648582ccb498c469d4f6dcf4140 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270875v1_alt LN:259914 M5:8a27dfcd4c4ae0239e3207b9994d8331 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270878v1_alt LN:186262 M5:07e6bd13f82b45996b0165c0a2c0ebb2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270879v1_alt LN:304135 M5:9fb8076a6ca93af66b2878d5cf07ebb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270876v1_alt LN:263666 M5:507bafbc0e637e7e5854acab4d2b7de7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270877v1_alt LN:101331 M5:b6724c024308122f0d19c875771d571e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_GL383583v2_alt LN:96924 M5:03ed78804fcfe33fb202c22d20e1ed05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_GL383582v2_alt LN:162811 M5:1919a95f3ea48fde56ba925295086028 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrX_KI270880v1_alt LN:284869 M5:e4ed2d1ef2ee71f00075ed9537aab284 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrX_KI270881v1_alt LN:144206 M5:6370851c900248567c9bc5bee75089ab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270882v1_alt LN:248807 M5:9a4ca55b5d44458dc5a1682ac6483738 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270883v1_alt LN:170399 M5:79916aa2c6a7669500b59ddd7b4eccec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270884v1_alt LN:157053 M5:28517f9e5682de5e74b42ba7c84a9d41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270885v1_alt LN:171027 M5:b5fc4688af8411394923dbcf6a85b85d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270886v1_alt LN:204239 M5:fae7fb0677c513b24e8027969233140f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270887v1_alt LN:209512 M5:3c672b8b13188d46ed1ea6806fdc9662 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270888v1_alt LN:155532 M5:325a22a47ae227dda0c8704cf4725601 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270889v1_alt LN:170698 M5:680f4977d9743df71085eacbff34769b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270890v1_alt LN:184499 M5:8862bbaae8fc895769859a6e89d19601 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270891v1_alt LN:170680 M5:0b1dd9ea37a3772563fb7298ad1f3857 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr1_KI270892v1_alt LN:162212 M5:24ba517f0feae1b6a95e1c65c21282be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270894v1_alt LN:214158 M5:0bfafe4b4a536ed3e5b77a89408d7534 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr2_KI270893v1_alt LN:161218 M5:d701ebc3d24a0056dc6b9418ba4e15c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270895v1_alt LN:162896 M5:4d08672ade726bd914e62a36d734da0c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270896v1_alt LN:378547 M5:751a447059436d9d5987f8417b2e2bd2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270897v1_alt LN:1144418 M5:5a8582c4943187c1a842801cf17d3706 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr5_KI270898v1_alt LN:130957 M5:9919f71320ac92b1abb42e2aedea84ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000251v2_alt LN:4795265 M5:e51d466e4a702acf3c9c5ffdc1ee0c51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr7_KI270899v1_alt LN:190869 M5:b42d6c28bfd9049414fb9d2617528db4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270901v1_alt LN:136959 M5:3d60ee1215e273e0c0267ad063614015 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270900v1_alt LN:318687 M5:80404f3147bd316898e6b5281f32c073 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270902v1_alt LN:106711 M5:f100803823a7cde3694698028e4c515c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270903v1_alt LN:214625 M5:5ce11ce469afc974c3024b30033dc5d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr12_KI270904v1_alt LN:572349 M5:6d8ebe7fb3dd3be62e34a1d2982e6f46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270906v1_alt LN:196384 M5:eb8157203f051ef7cfcab1f4647f22c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr15_KI270905v1_alt LN:5161414 M5:31c97360f2a53ab92bd8d6db1b1a410d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270907v1_alt LN:137721 M5:f5a65eb09de3017536d1b7c0b2237fbd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270910v1_alt LN:157099 M5:4eef5dbfe56e13c810780221b0f6426d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270909v1_alt LN:325800 M5:6a46afe4c5e257b556f25c37a09fc024 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_JH159148v1_alt LN:88070 M5:bd147af8e713f365ff05352eb8a4508f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr17_KI270908v1_alt LN:1423190 M5:ef3f9ebebe7ad9f9690daedeae8a74f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_KI270912v1_alt LN:174061 M5:3a8acbc0c5c751ccad11263a608715fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr18_KI270911v1_alt LN:157710 M5:930ee72eaddcaf1355220cd39539c326 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949747v2_alt LN:729520 M5:10169e6e86933b2bf74797be020fc857 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KB663609v1_alt LN:74013 M5:54abe159678a84e88ceb2d5271027628 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrX_KI270913v1_alt LN:274009 M5:a4d4a5e5b5da1e65f007116b2797a4f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270914v1_alt LN:205194 M5:086e17b28e2ac14e602c5dae2c358364 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270915v1_alt LN:170665 M5:a5c43ab0e4289f3951e65fd57419782a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270916v1_alt LN:184516 M5:45199873c7617fab0ba00e85a43ddbeb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270917v1_alt LN:190932 M5:1595cb5d2e776d8a505a06db45ea2c04 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270918v1_alt LN:123111 M5:e79cb3af945f28de17d744bf24cde080 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270919v1_alt LN:170701 M5:a25ce4a282c7ead76bb2468a9a56d082 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270920v1_alt LN:198005 M5:009fb96b4ee6d64732dac95a1f380f41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270921v1_alt LN:282224 M5:4e9f360581e618964d379d67e3b51f90 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270922v1_alt LN:187935 M5:7b6dc564b92bed8df5de2c1fa8de0986 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270923v1_alt LN:189352 M5:5b0339dbf41b0301512639781d44d546 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270924v1_alt LN:166540 M5:944759f109cad94523d1f3918ac0b382 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr4_KI270925v1_alt LN:555799 M5:08b3f5ff9beac37e3bbc50767cf3b43b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000252v2_alt LN:4604811 M5:0c2ee784f397289b1713ad133fd93d39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr8_KI270926v1_alt LN:229282 M5:58a00f9d6216734f0bf36a0f2294c246 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr11_KI270927v1_alt LN:218612 M5:159fb3a622d456897c41d93f4627d169 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949748v2_alt LN:1064304 M5:21fd288470eea03e7424729134f472f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr22_KI270928v1_alt LN:176103 M5:efcf7ce77388977478ffe2757b8f8430 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270929v1_alt LN:186203 M5:9f8d51d9c1ec4887cd55a1d0d8e61469 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270930v1_alt LN:200773 M5:2c5abf84ad91658f7262dff7ade3e7b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270931v1_alt LN:170148 M5:36b9336df72c820b6efa95567a5aaf28 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270932v1_alt LN:215732 M5:4a92a470a7851edf0c238a74814dece5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270933v1_alt LN:170537 M5:413442cc380f0bb8f15b33bc25149af1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL000209v2_alt LN:177381 M5:0e7a0f548f1f770b4272be1bd8b018fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270934v1_alt LN:163458 M5:43c32fd3aae0eee20305d3d735e7bbb4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000253v2_alt LN:4677643 M5:a5cdd062ee0d61ea6b3c5166e79b472e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949749v2_alt LN:1091841 M5:cf8641cadcdb22174c55a5d9d5c46036 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270935v1_alt LN:197351 M5:8175b26fd25c1671efd5af96e8b44835 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000254v2_alt LN:4827813 M5:ad357767de6beef959952d39200522ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949750v2_alt LN:1066390 M5:c48b453696107dc7105bce3857e1cbc6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270936v1_alt LN:164170 M5:64f7f27938eb4c80140f34e3d2aed483 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000255v2_alt LN:4606388 M5:bceeb4190283066a6d0891562b41d164 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949751v2_alt LN:1002683 M5:1615d0a6f8a5ff11274ba2e00b9487a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr3_KI270937v1_alt LN:165607 M5:39b5207a79f67fb863be3b2a5bed0963 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_GL000256v2_alt LN:4929269 M5:a80b1b3c21ac7e7b7acf75178ae3d83c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949752v1_alt LN:987100 M5:7d007a35ff02e56325881c68bb17b565 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr6_KI270758v1_alt LN:76752 M5:9d0d4a56d72f18d39c7a1094deb177a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_GL949753v2_alt LN:796479 M5:19162055ca3e800f14797b6cd37c1d4c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chr19_KI270938v1_alt LN:1066800 M5:9363b56f7b34fb35ab3400b1093f431a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrEBV LN:171823 M5:6743bd63b3ff2b5b8985d8933c53290a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707606v1_decoy LN:2200 M5:20c768ac79ca38077e5012ee0e5f8333 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707607v1_decoy LN:3033 M5:444cb839a910d9af9b5c5f013cb31063 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707608v1_decoy LN:3112 M5:bc263c9aacc51dabcb0ac4987c416a0a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707609v1_decoy LN:1642 M5:ec7a95a8f6e3d63152c0b1f46601e2ee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707610v1_decoy LN:1393 M5:441a56ef62eccbc2ec6d0384fe40367e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707611v1_decoy LN:1103 M5:a5378db83cbffb5f31da4a549152cf80 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707612v1_decoy LN:1039 M5:4ac166fdb188d87a64b59e346fc040cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707613v1_decoy LN:1619 M5:af5b7705364129a1fdf718e223e8dfb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707614v1_decoy LN:3122 M5:67860fb1c59453dbaf2bc21834f76ff1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707615v1_decoy LN:1934 M5:e8773170190290d21bf5c7bac36c73ee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707616v1_decoy LN:3111 M5:9792c2376ee383fa1d0763ff6b391fd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707617v1_decoy LN:2545 M5:7d9f68ca65f2e9029ef8c2d352b6068d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707618v1_decoy LN:2295 M5:dfec4823eafc51fe89a1c8a61a7861a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707619v1_decoy LN:1551 M5:f0f821c64e2f1339f4452cfabcd1a247 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707620v1_decoy LN:2046 M5:9e54f11658064725f046e3ffc7f5166e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707621v1_decoy LN:1222 M5:4053fd7005d4e430ff32b9b0c9dda52d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707622v1_decoy LN:1535 M5:393bc14f66f0af8c20fdd93b1a3dc78c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707623v1_decoy LN:3784 M5:5da25a3d7060acf52ce5d2084d8922cc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707624v1_decoy LN:1329 M5:18daae4566bfb62823b548ab88fa894a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707625v1_decoy LN:1238 M5:8a05b8842f52e87406e28162b8fa7249 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707626v1_decoy LN:5623 M5:e6705349b3e2839fcd382596f831045d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707627v1_decoy LN:5821 M5:cafc12fed95e40d4c1b441ad93f0493a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707628v1_decoy LN:2960 M5:1e0a770fe11a74301ab4535bd9be16c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707629v1_decoy LN:1848 M5:5288216fe388c6966e89e6f8a3a5118e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707630v1_decoy LN:2315 M5:1cab33f8538d939c751e2b2d9b01957a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707631v1_decoy LN:1945 M5:099f236a02fe8e80d7f135727589bce1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707632v1_decoy LN:1424 M5:a2e305f020577c5f7806c411f995402c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707633v1_decoy LN:1274 M5:967deda0c966a0bcd27c6e047e18e7f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707634v1_decoy LN:1007 M5:093928d5a51ad63c286ec229982828ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707635v1_decoy LN:1414 M5:270f3e796a1b7c345e83762fa735bfe1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707636v1_decoy LN:1725 M5:f00ca03da369b2dcf101bda70773022b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707637v1_decoy LN:5354 M5:ecf9ac0af0f7009b8b4e5fa639e7fc97 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707638v1_decoy LN:2189 M5:5a928a8e7ad5ec7808ae7dbcc7ba72d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707639v1_decoy LN:1294 M5:692623b127f6621794d92bfc65f198f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707640v1_decoy LN:1831 M5:23d5c8473f768ca2c6a3eed843fc2cf2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707641v1_decoy LN:1647 M5:09778e8253cf377911db86530bec54a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707642v1_decoy LN:2943 M5:6759f7bb4098b6eb0ec14f5c63369661 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707643v1_decoy LN:2857 M5:a6d7044f6c244253ec1e142bbb35d859 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707644v1_decoy LN:1030 M5:e3e8c02e17ebf79c0e90ce41a16ccedb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707645v1_decoy LN:1070 M5:26882fb498a5b281266a48f82726a7c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707646v1_decoy LN:1735 M5:b120aeee1c12ed6986a9743badfcc505 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707647v1_decoy LN:1982 M5:d1d012b49d3cb20db627f6dd65326217 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707648v1_decoy LN:1564 M5:a4fae6ab9f74c3f1b2c0f8352e9a3519 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707649v1_decoy LN:1775 M5:4bcea8af13f18547fe066845bdc1c120 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707650v1_decoy LN:1540 M5:b4256e83f91ba0174562138c3b466a45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707651v1_decoy LN:2013 M5:f7d9c80ab1716aee1a2237dce70b768a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707652v1_decoy LN:1176 M5:4af9bb8c4125407fb9f03e5fc7798528 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707653v1_decoy LN:1890 M5:854efc081d295baa5a1efa0fe1f9bb3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707654v1_decoy LN:3644 M5:5c90649c1bc38e0b333381ac593252bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707655v1_decoy LN:2785 M5:407de1fa7cb87b88317888d98ea56599 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707656v1_decoy LN:1017 M5:f521affa49ef813fea48a8f5b1fa8e13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707657v1_decoy LN:1068 M5:478f244a134bc02c1c51f63009626864 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707658v1_decoy LN:1007 M5:f4ce5e4a861ab83037e05e271e74497b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707659v1_decoy LN:2605 M5:5c8b18731c2eeda273e0caaf2e878654 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707660v1_decoy LN:8410 M5:b581d341cdf7f17875e60e3da33c398b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707661v1_decoy LN:5534 M5:fd57c53936fe1011e42fc08ef3ff65c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707662v1_decoy LN:2173 M5:5ca2d18676a26044b213465e1e6ac206 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707663v1_decoy LN:1065 M5:07cb60b1a2ff813eed23c7cc4128baa7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707664v1_decoy LN:8683 M5:ac12df9c6d81003f812323327f937147 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707665v1_decoy LN:2670 M5:366d796fda2fe9f8baadd88deddf9dca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707666v1_decoy LN:2420 M5:b5c696c469b2e831e8a5e8e87c6813e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707667v1_decoy LN:2189 M5:1f055f8a1a1f8c356b45cfcbd6db6713 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707668v1_decoy LN:2093 M5:839cff5bd33068228bd1031bf8be9a64 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707669v1_decoy LN:1184 M5:ad69f6deb582834c638527c565c4de2e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707670v1_decoy LN:1205 M5:aa9070a191ece87a421285b5be2e8b68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707671v1_decoy LN:2786 M5:28c96decb9c1d76b9a76b1ee75e2421d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707672v1_decoy LN:2794 M5:4d547ee98d0678f832df5c46f91604aa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707673v1_decoy LN:19544 M5:2d2080522d0d729d94c7d73eda00c62a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707674v1_decoy LN:2848 M5:9182728e5875ee5cfba2c524c997fcbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707675v1_decoy LN:10556 M5:021a0164c68fd77f51bead9e2c4e9cea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707676v1_decoy LN:9066 M5:c8c71f6c1f2612fed812e69c737ca6ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707677v1_decoy LN:7267 M5:0e929202e653306730239e7dbea8a861 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707678v1_decoy LN:2462 M5:21e3c2e28a7980351470a14a0d48160c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707679v1_decoy LN:1774 M5:9f0f7ecdb5b097f83aab97fff76127d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707680v1_decoy LN:1297 M5:3eab486a1f41712d26b8c251ad9dbff1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707681v1_decoy LN:4379 M5:04aec18ad103fe46337c6643d0eaca0c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707682v1_decoy LN:4208 M5:b413a0a980c7752db350c74c5f97937b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707683v1_decoy LN:4068 M5:80a8f28af2c872b62345c85f6217dea7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707684v1_decoy LN:2940 M5:774582f989e0cda16927addcf0ece955 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707685v1_decoy LN:3938 M5:cd0020e224ec3edc01ecdfd648f4099d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707686v1_decoy LN:2072 M5:879ef8f0e126b3f557a0170b71459c6e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707687v1_decoy LN:1136 M5:69aff37696b0d4542e56fcf9ad9af43c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707688v1_decoy LN:4248 M5:d301756f721c9141f1216e52fa4003da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707689v1_decoy LN:5823 M5:f29516cc0ba82ac48516c65b100b2255 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707690v1_decoy LN:3715 M5:a154584ab9547c044251e469591cfd94 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707691v1_decoy LN:4885 M5:ab7f60b71af56c68e4a0f56174c90e4e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707692v1_decoy LN:4813 M5:e8f5365670fcf6e3612d2e77ba943aaf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707693v1_decoy LN:2899 M5:3cf4cd0669a4fc846ef0aa0aac7cb739 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707694v1_decoy LN:1228 M5:38a9e18c0e3208c8b593dc4fdf118a9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707695v1_decoy LN:3119 M5:ba7aa2faa9f2fad7ad94f0acd152e6f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707696v1_decoy LN:3828 M5:4b39179f8d3eefee8f4865bf6d11dd9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707697v1_decoy LN:1186 M5:0b3eecab59c656d96325f01e70a906c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707698v1_decoy LN:1908 M5:708018246fdce7d4b8f47502bfdbc3a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707699v1_decoy LN:2795 M5:001979586db1b719cca6d3dc0e50e71c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707700v1_decoy LN:3703 M5:5c692cc6be5262461fdbba8574e6ff40 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707701v1_decoy LN:6722 M5:378424a19c93bf19eb0f565cba923eb1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707702v1_decoy LN:6466 M5:1b5a6efaa31d7974dffe73fc4bb88349 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707703v1_decoy LN:2235 M5:c045d23c4c9497849b45c45423f705ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707704v1_decoy LN:2871 M5:b44844f0650f1953d042b7b0c9740cb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707705v1_decoy LN:4632 M5:6c1d769d4a84500b2eefafe1cf460164 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707706v1_decoy LN:4225 M5:d37d4a4d41be60f45aa967ecac1a0c5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707707v1_decoy LN:4339 M5:ffcd631ed0bf56e54cd72ed51c70fa3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707708v1_decoy LN:2305 M5:6ebdd668b564460303637164213d0dff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707709v1_decoy LN:3273 M5:3531b30c5833b2f37585aeabefeafe36 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707710v1_decoy LN:5701 M5:943eba7b0fd71418d0ef4ab2bdcc3f14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707711v1_decoy LN:4154 M5:8e6daf77c80fc9e8710c6732e9d72918 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707712v1_decoy LN:1243 M5:65e4a30840d6420ebc8b7681d38ae431 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707713v1_decoy LN:1308 M5:d9aafcd2c4b12f82a113eb5418d120ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707714v1_decoy LN:2922 M5:4904d632dac614c608b8b7e3478dc3f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707715v1_decoy LN:3044 M5:15707c34a9803e1349a621fef2605913 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707716v1_decoy LN:2888 M5:68339f161a66b364c5e9ffed664272f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707717v1_decoy LN:1742 M5:1fd1325fe768dfe31d9199ee60b60206 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707718v1_decoy LN:4969 M5:454864584e92f6e195416ac9f9a8ee38 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707719v1_decoy LN:3270 M5:10feb2ed6ea7480290058dfc77385541 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707720v1_decoy LN:6028 M5:ab8f184e44faa3d93d59d9e3beba3acc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707721v1_decoy LN:1105 M5:97e3e0ba281c40270062097594c95b2c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707722v1_decoy LN:2884 M5:983f9666298bffa6bb3bc09593f83ab7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707723v1_decoy LN:1124 M5:d1d03191a7579c36409ca1686af957c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707724v1_decoy LN:1454 M5:648298f5e7a06c49b911d520d02036f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707725v1_decoy LN:2565 M5:507d9e32ff14b42ffc67abc3e66f8420 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707726v1_decoy LN:2149 M5:2cc36819133158e39f0ec48e7cadeb08 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707727v1_decoy LN:2630 M5:2ce79b6f499673a5546b3b608cc6d262 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707728v1_decoy LN:14625 M5:5c193cc3e52b61477ff6efd7d6bf1168 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707729v1_decoy LN:7431 M5:a6530e653f0ed096c60f6d44f83a5ac3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707730v1_decoy LN:5776 M5:8131fcdd109e0c725a9cd0f4dc240efa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707731v1_decoy LN:4820 M5:e86dbde0af3f5ba60b0ca212f4e51b75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707732v1_decoy LN:1227 M5:326c77e23881e6196b8441b3a96fc1d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707733v1_decoy LN:7503 M5:c633c411b0c6959cc08d71120f604c30 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707734v1_decoy LN:9652 M5:97472a33b38a6285aadc3ea8e200a0c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707735v1_decoy LN:1091 M5:8eb7237ab5103bf046f0b4fd8956e506 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707736v1_decoy LN:2467 M5:90141a455f60c4f02dc064d0200707c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707737v1_decoy LN:1270 M5:40c2e03cacd0e24267f2f495997de86f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707738v1_decoy LN:4365 M5:5a1da2ab0c8d8ec4e368ecdcb314a951 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707739v1_decoy LN:4284 M5:62bdd88183d94495bcb948a7c17e045c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707740v1_decoy LN:10282 M5:d20046e860cc9e220ba67181d6317b0c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707741v1_decoy LN:5601 M5:8eedf1585943eb4a63b18a14c9d60e08 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707742v1_decoy LN:4758 M5:453aaa1ccce2e7bcd76462fc6fb82810 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707743v1_decoy LN:1624 M5:6b23b3de3fd00f6eae35fa84c9f0a1f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707744v1_decoy LN:4024 M5:a5fc63b6fa7d72420c481475f1a9b25b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707745v1_decoy LN:1276 M5:1aa689253b47a87a0497755453cc3064 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707746v1_decoy LN:5083 M5:4df51fadbde93b81c9ce73ae34ad299d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707747v1_decoy LN:2075 M5:b020b9e14b2329811d8020661a1b223b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707748v1_decoy LN:3553 M5:3aae97b73e2f50aa215741927751caf2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707749v1_decoy LN:7010 M5:5f573deb341bf1ebc60148d185f3863b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707750v1_decoy LN:4718 M5:c90f362bf73d35d5b7ecc0df55b8e7f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707751v1_decoy LN:3546 M5:0a4232c675b33b2779a9f54185a01bf8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707752v1_decoy LN:2873 M5:dffaf05b9fdcf311b6631694c9a7f721 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707753v1_decoy LN:2144 M5:f62da5d7fb084c6276e9144911369e38 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707754v1_decoy LN:2243 M5:c01ba9f403c104a17c7a56c2e246402b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707755v1_decoy LN:5343 M5:fc0967e1b54e87ee9e6649ee9e135bfd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707756v1_decoy LN:4877 M5:7cddadbee2311357be0a6cbc21b7c3ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707757v1_decoy LN:3034 M5:8bbd8d0206582bdd7ff0d98834c11701 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707758v1_decoy LN:2826 M5:d1fe878facfdb974e00aa366bf3f6574 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707759v1_decoy LN:1221 M5:f538752a6b30c7db1def6888006fdf82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707760v1_decoy LN:1169 M5:45388eebdbbcd5727ecb94a69d2b09dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707761v1_decoy LN:2319 M5:880e2410158ce2b1647e1b9d4e80632e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707762v1_decoy LN:3450 M5:1a94da832f9b0b1e2cf7e642869d2b68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707763v1_decoy LN:2674 M5:98597e188d3ba588151f6808ded88a91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707764v1_decoy LN:3912 M5:5b188ee33158e7afbe044f110d28e434 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707765v1_decoy LN:6020 M5:487d6332ace17c42a8f64aa9adf10de4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707766v1_decoy LN:2303 M5:05f988a4a7180849c6c81602c29279eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707767v1_decoy LN:2552 M5:4f8579ec6353f301ef8362afb14c9e1c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707768v1_decoy LN:3656 M5:dd57001db92b45e0677136beb49e978d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707769v1_decoy LN:1591 M5:cae5200b9ceb32d63e9183210ec694c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707770v1_decoy LN:1209 M5:fde0c42984e1da0ba9aada4b168c4913 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707771v1_decoy LN:3176 M5:e48eeb05bf1913704ee94aad24e95e39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707772v1_decoy LN:8915 M5:e41a1182f28ef302e03876ec46b7ba76 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707773v1_decoy LN:4902 M5:61cb73463b0096ce7f34215a0e9c7d2b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707774v1_decoy LN:3324 M5:d0d739ef87156a3f1b0083d560547169 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707775v1_decoy LN:5997 M5:b23670b667f938d11721f9239ce46676 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707776v1_decoy LN:2618 M5:51e373cda128d4d44cf38c8c0057966c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707777v1_decoy LN:10311 M5:c35ba91babc524c1b3270599ece90957 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707778v1_decoy LN:2440 M5:f3bed9c00d53e9777a38a27156dd1659 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707779v1_decoy LN:12444 M5:bf5be5d1c5a27ad4ae37d468d9bf5990 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707780v1_decoy LN:5691 M5:2813badf81db953a968eb7d49bc2882f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707781v1_decoy LN:2717 M5:0a1557b49527e901599bd44d72da8e1b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707782v1_decoy LN:5277 M5:4061182ba5da9755a2a25e68a304ac01 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707783v1_decoy LN:4373 M5:3eef41e80ceccdcb21b9dba8a2cbca5a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707784v1_decoy LN:3224 M5:ef2e57d4220e2cf9680eb6cb27022a8e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707785v1_decoy LN:2631 M5:3ae2eacb39d96c2b69c8036fa48550e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707786v1_decoy LN:5385 M5:cba39df25869787d7270324f4c2e11be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707787v1_decoy LN:3678 M5:ee5041cbefd1339ffe625fe56a2ad8fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707788v1_decoy LN:1412 M5:cd5878fb701bca2a77f7f0479ada562a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707789v1_decoy LN:1443 M5:098268af4ad1b71ed7dbf4648ea026a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707790v1_decoy LN:1098 M5:437c3cf69fd5e7568ba6cd5302f1b0e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707791v1_decoy LN:3240 M5:b4243a9c38158ab50bf873d91a699467 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707792v1_decoy LN:1915 M5:44e6058f98843778f5708a68a650bd10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707793v1_decoy LN:4667 M5:1664dafd7db46e3d2e9257978a3c0282 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707794v1_decoy LN:7219 M5:69855847368a4c0722753f3097f73427 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707795v1_decoy LN:3277 M5:21882b529bd5fd85dd805de6c51c1d58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707796v1_decoy LN:3473 M5:386834c5cf2dacec8dc5f75898909eb2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707797v1_decoy LN:4243 M5:4d8a0b4dee134f88bc4604b841d38ec4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707798v1_decoy LN:17599 M5:6ab64c474c333b7a8e0aaf72d39306a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707799v1_decoy LN:5095 M5:f1c458ebccd70c843cec05e23921bbf6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707800v1_decoy LN:2237 M5:0feef89c01eacd296069717f5c7a7c71 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707801v1_decoy LN:2901 M5:27662470b962dfea212da33f14909142 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707802v1_decoy LN:2666 M5:1eb4be22c4e6d37a91facfb3025520b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707803v1_decoy LN:5336 M5:db6d33944d47e02a8df7cda94a1e996c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707804v1_decoy LN:4383 M5:bd1e2dc89c9dd82b43d3787afc9abe47 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707805v1_decoy LN:5446 M5:4103eb12b8c3ebec9cd8fe2f49b6d1e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707806v1_decoy LN:6252 M5:9218b0dc97e8ccb069eba3e9d676a995 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707807v1_decoy LN:4616 M5:4d717a57114c8f1b9b4ffb96edcd6615 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707808v1_decoy LN:3021 M5:eaaf056266a70e2a7be4db848d68672d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707809v1_decoy LN:3667 M5:4c8c58d51417cec07e970c76f7b280d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707810v1_decoy LN:4563 M5:4bd16dfab458c175a83a781d1b602d8a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707811v1_decoy LN:1120 M5:f63cbec018752a7584fd25ba859e6807 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707812v1_decoy LN:3845 M5:47464e332307f876b9138d74f8726752 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707813v1_decoy LN:2272 M5:c5516af17e36a2e09f21f2f4120377e6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707814v1_decoy LN:4764 M5:5d190274f9c28c4642fe1f75b5e3c9f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707815v1_decoy LN:5410 M5:c87029fcb5e0f94edf755e7b6b18f89e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707816v1_decoy LN:7150 M5:e2a7085047a795f4ee89232573d43df7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707817v1_decoy LN:1762 M5:2bf3e07e17fb50802f7da42c78322860 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707818v1_decoy LN:1207 M5:db2fca33bf9e3730c72e754f7e536e79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707819v1_decoy LN:1331 M5:b96ffe2a3a7a75977f2286d021069bc7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707820v1_decoy LN:8307 M5:89d9580408ce4fde62950344c2daa448 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707821v1_decoy LN:2276 M5:2a9fffb25e3ccf46680306388d4c2e6a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707822v1_decoy LN:2575 M5:8e5278c0a004efc105d35744a6aed334 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707823v1_decoy LN:3970 M5:ef86294088659e3b6c564f7a2c94abd2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707824v1_decoy LN:1352 M5:af1ddf1aad7bb03a0c72d325e8e9a52c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707825v1_decoy LN:3040 M5:673e8681ea9b0fe7b4b74a011c7780e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707826v1_decoy LN:2070 M5:83ff186bce877c493f356e110486b13a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707827v1_decoy LN:2913 M5:156af338c90535ad8ddbebc3cc2b8e06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707828v1_decoy LN:2389 M5:dec17b4db1503252e0d25e88839aef19 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707829v1_decoy LN:1835 M5:495cf270e534859f024d452fa5714a83 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707830v1_decoy LN:4807 M5:9d8e879d40a82477b244fe7baf4f1603 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707831v1_decoy LN:2201 M5:661353dc86a8b62e524ea43ee3f39c6e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707832v1_decoy LN:1265 M5:e33ce5ec06d99813aca09515f057e64e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707833v1_decoy LN:1961 M5:93e5f197aa47e632cdc4f4f9a06504c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707834v1_decoy LN:1064 M5:28a2c878efec380df24a5544738eda91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707835v1_decoy LN:1932 M5:bf3bff12edc1fb4331c341b1b2044e32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707836v1_decoy LN:3213 M5:d47bed4a51e112b3d415b77d354290ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707837v1_decoy LN:1178 M5:2a4f6a9f9df49215bb56bfbe5d6e1990 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707838v1_decoy LN:2926 M5:5f28eba962c1026228afdff4fad6ade4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707839v1_decoy LN:1038 M5:b42a95db827e89510d3dcdeac5fba1c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707840v1_decoy LN:3298 M5:54dc58ad6f4d92475e298f41206a1118 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707841v1_decoy LN:8992 M5:176684391390b0afaccaac0eecdb9560 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707842v1_decoy LN:6698 M5:b309273a9a78c30bcd5a5573742fd06e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707843v1_decoy LN:4880 M5:3ad4a232fe303ab7c09600c91b6c406b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707844v1_decoy LN:1766 M5:85cb4f0b6ab4d7dcec41ef37ca81ead2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707845v1_decoy LN:3532 M5:fa5f7bc4de1539f23d5610c26d69ed49 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707846v1_decoy LN:2297 M5:2bda3374523a39aa9c8766059468392e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707847v1_decoy LN:1234 M5:23e58f01c32ab1539dc5738c36b70940 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707848v1_decoy LN:1205 M5:c9b75dbdb9a2d4ee4648ed7fb537fb0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707849v1_decoy LN:2790 M5:20b3eef55d3dad7a5dbcd68268dad36c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707850v1_decoy LN:2006 M5:67d720cd69f97ad773cdcb3ccde8d47e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707851v1_decoy LN:4593 M5:7c836d280ec9794d222476d02e21b416 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707852v1_decoy LN:1579 M5:ab4542b394c8e1133f8eba10d896cb44 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707853v1_decoy LN:9597 M5:9d6b74ff3ae40b1134f4b1f1eabe16ff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707854v1_decoy LN:10451 M5:ec7353db2ae0657678d29512a5b78b59 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707855v1_decoy LN:3219 M5:b522f004eb3c1bdf17fe74e82e23ff88 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707856v1_decoy LN:2300 M5:7a18b1dc033c5a406a5f752e8a7d5a6d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707857v1_decoy LN:5985 M5:28c60286011df7faa5b7167d8dc7a5f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707858v1_decoy LN:2959 M5:682104fe067bc0a5e18763b5d0eae010 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707859v1_decoy LN:1340 M5:3b47b99a35fcc0a437518350a37c97c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707860v1_decoy LN:3148 M5:cc961e4253553c5534e478b629b89930 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707861v1_decoy LN:2242 M5:beedf4d2ae00fca7cc2cd7e9d4ce6012 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707862v1_decoy LN:16513 M5:968c6df8aaaaa1015e74f591e4722ed5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707863v1_decoy LN:7821 M5:9c8e1c87e1b58622c67edbf732b7ce9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707864v1_decoy LN:2159 M5:37c9ce7cb170bed17a0deb982f64fefc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707865v1_decoy LN:2114 M5:8b637137aa5f04b43be61da029e5f818 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707866v1_decoy LN:4109 M5:1d5a765b813cd25ff873c52ca2b47f05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707867v1_decoy LN:1544 M5:c2744b7344993ebcf11d21cb098cace1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707868v1_decoy LN:1005 M5:241518a7a0343d82a0d99b1dc00e5d79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707869v1_decoy LN:8632 M5:010737c4caab4d5e629753ad40bee3bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707870v1_decoy LN:1012 M5:88b3e83459db1925338cff6156325503 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707871v1_decoy LN:4728 M5:dcab24555bd79f11062bd6e856a1db4a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707872v1_decoy LN:2165 M5:aff31850364e11f37ba76baa5b7164d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707873v1_decoy LN:7591 M5:b034989a9fb62216a2547cd1206f4185 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707874v1_decoy LN:5202 M5:1e514f0c86c918e80fa4b29c206cb8cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707875v1_decoy LN:4241 M5:da66844cc4d4846716264b16f77233a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707876v1_decoy LN:4131 M5:60562638c91c3b3655e2ab9544600425 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707877v1_decoy LN:2272 M5:d672677153fa65839a94ae64d9284d49 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707878v1_decoy LN:2085 M5:d1bc85197aa7215a4b0248a74c19b399 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707879v1_decoy LN:4346 M5:1999b7a1b498a212e212e60377adb20d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707880v1_decoy LN:1208 M5:ea9da47963d703e465aaff5693250f76 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707881v1_decoy LN:4543 M5:e84492cd2aa22936e00b280fcaca8212 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707882v1_decoy LN:2772 M5:bed5000e1788ade3ad5a8541cf1c1671 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707883v1_decoy LN:2490 M5:3d040ea1d7a17b15bf44f59b8e66c98c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707884v1_decoy LN:4568 M5:bd5a8cfffb4d5bab0900b4fa1fbf9390 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707885v1_decoy LN:1776 M5:f0f2b210ad8e779eec5a4ba478c83ef7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707886v1_decoy LN:2699 M5:91fad31e963c9f7821cf37705e55f40e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707887v1_decoy LN:3534 M5:f6d25106c5942148df4207ebc4a60371 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707888v1_decoy LN:2424 M5:4857413cb43abc01f2f0c2d56e196332 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707889v1_decoy LN:1747 M5:e1946468f8d77d4ac72dd72fe8ce04f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707890v1_decoy LN:1088 M5:698423687f8bc007b40065a2658b7360 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707891v1_decoy LN:1143 M5:3dbbd76127230ad72adb348524c250da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707892v1_decoy LN:2530 M5:8909228bbdb37a62a906447c5b24616a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707893v1_decoy LN:8049 M5:a4e81d020fe37025f26c26aa8241f26d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707894v1_decoy LN:1366 M5:69423d07e5d284b7ad365c144aec6f4e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707895v1_decoy LN:4284 M5:da455b511b9da89b4527ee045c5fe231 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707896v1_decoy LN:33125 M5:10522217e43c9d9228b02fc14c418df3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707897v1_decoy LN:2137 M5:962521d05f6b98894c65d3669ba66008 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707898v1_decoy LN:3840 M5:561d50151a7132f385be0617165bcecb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707899v1_decoy LN:3087 M5:46cc4f61d95475ad4c963e9bfeb206fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707900v1_decoy LN:2041 M5:cea01cf71468a810b970138149139764 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707901v1_decoy LN:3344 M5:ef03bb9d9f28ee51bfc7291ee41812e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707902v1_decoy LN:2921 M5:dcc9f313bc6e72cc000ac62e62e975f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707903v1_decoy LN:6581 M5:6346aa1b0e3055e121b9cdccb04a53fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707904v1_decoy LN:3968 M5:ed15d6545609df15a0758922f36c9f87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707905v1_decoy LN:2339 M5:af66f6d8d03e1c43cef4284aef73ed96 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707906v1_decoy LN:1243 M5:bec4227b2724132e7ac912e4532bcf8d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707907v1_decoy LN:7776 M5:a9e8cc852787e0a7dea34bd16fd28b35 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707908v1_decoy LN:19837 M5:63f4cfca34f0fcd66c826ccc8c6d6cbd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707909v1_decoy LN:1737 M5:0eb45f3727a363ec209755519593220a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707910v1_decoy LN:1098 M5:bda7161c475d6f7bae40ce030d70fe87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707911v1_decoy LN:1893 M5:80bef2e1b21000a202a974145a551fe4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707912v1_decoy LN:1281 M5:96d1e0d5628111d9566a6da789c6204b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707913v1_decoy LN:1527 M5:ad42dca85dd0540c9229a86997665245 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707914v1_decoy LN:2055 M5:c7f0324e70cef84566fa5453dcb23f5d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707915v1_decoy LN:2527 M5:f7d3cdbc2f96d05bc8ff741d46f2f687 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707916v1_decoy LN:3275 M5:d9702219a1b8982c63cc1d6238368ab2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707917v1_decoy LN:1265 M5:6df0a4af8c86a2528464f45af7cbaccf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707918v1_decoy LN:2623 M5:5c6afdd9afcdaae5399f684978746b65 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707919v1_decoy LN:4850 M5:aed47145c2269b8c33a71652002e351f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707920v1_decoy LN:3584 M5:47dcd6ad41f139cfd6b9a02581cad11c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707921v1_decoy LN:2561 M5:33902e088970543e1715ee99abdcf7b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707922v1_decoy LN:3041 M5:313f5e86963d937b91534f4895c3a590 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707923v1_decoy LN:1409 M5:6af5cfeb86bc84ecdcfb91e994b4bdbb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707924v1_decoy LN:4596 M5:3415bc1f3a762bb7077c328b9e9e723c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707925v1_decoy LN:11555 M5:10cf29455a308a0380471d6bce16f4a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707926v1_decoy LN:1266 M5:ed2740c08c55e9dbdd3cf9db469d0df6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707927v1_decoy LN:1079 M5:7d006b7ffa2791321663578e50f456f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707928v1_decoy LN:1087 M5:e6a75cbd458016b1643e76ecc570d33a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707929v1_decoy LN:1226 M5:21256ce4fdadc803045a9b8f60dfe16b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707930v1_decoy LN:1131 M5:2f7c4c54c98b465c14120d32e5d60362 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707931v1_decoy LN:1199 M5:68471da27aefc5565f37a51052c09c8a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707932v1_decoy LN:1084 M5:ff5f2aa407bb1f0573a4d815fb69f917 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707933v1_decoy LN:2038 M5:1b9a682cbf0c31fe8f29a4aa0bbef274 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707934v1_decoy LN:1070 M5:f8c939429a099c8eaead4319b5ecd2b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707935v1_decoy LN:1312 M5:00ead3e9dd86f6d5e7d499e285b509b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707936v1_decoy LN:4031 M5:9175dddf96d1e576065b2465c6e2b567 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707937v1_decoy LN:7445 M5:f83991a23f7bca6045f72a0bf34ecf13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707938v1_decoy LN:1770 M5:d7487b3fc5cbe693cdcd5ef883b5f00d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707939v1_decoy LN:5600 M5:5fc39cbf76db7d0c1d56123a313a22c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707940v1_decoy LN:1882 M5:f239e0af7db0be33b82e8e4dc2405165 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707941v1_decoy LN:1170 M5:e7c47b0be4905c7dcf70b8659a334abb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707942v1_decoy LN:1300 M5:1beccd3ea3988863bad1b7614a1db1e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707943v1_decoy LN:5325 M5:54c4ba54e1ad9eebc7832dfb6cf778fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707944v1_decoy LN:2043 M5:669c152c4b3e10d1f61d5afb2cf22bcc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707945v1_decoy LN:1072 M5:d9416b21be3f6603426526a22e7e558e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707946v1_decoy LN:2463 M5:ce498b9d34e1447317c0d5d27aa0f911 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707947v1_decoy LN:1010 M5:4183a38dcef3c56b79dcf7eae090e37f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707948v1_decoy LN:1432 M5:c261bf235424d74a7b1d8624bb8d6ec2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707949v1_decoy LN:1162 M5:26aeb5ffbd148e35fa48fee69317843f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707950v1_decoy LN:1095 M5:2c795c49d9b77f2b5c0ef5c97e76ae46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707951v1_decoy LN:1118 M5:a0c0ea97493f7c511c5b0af1e8913e92 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707952v1_decoy LN:1383 M5:fcc78a7bf9b47bb52730911e285ad0f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707953v1_decoy LN:2289 M5:ba3eede08e65f36da588370a142cc02a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707954v1_decoy LN:1648 M5:de71b9c9ca48f28bc0c9d13c5139ce68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707955v1_decoy LN:2203 M5:4c9964a811b62ea018169d81ba474499 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707956v1_decoy LN:3270 M5:316a59db52a456d6aa5e140e1b1c8794 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707957v1_decoy LN:11499 M5:cd3bb1a0e33bc1aafb51df407fcf16df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707958v1_decoy LN:2474 M5:5909618b2a56c4db15b017bfff248a13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707959v1_decoy LN:2294 M5:bfd4a30a41e5e647cba935da2e8a63de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707960v1_decoy LN:1238 M5:8025797164ec304ff940e4ad94643c70 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707961v1_decoy LN:3410 M5:02a40e206c78446d368b6001622aa540 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707962v1_decoy LN:1523 M5:7f11ba71c0814d0ca2b3839d55de672d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707963v1_decoy LN:62955 M5:d620b771cabe5b2cc4b943ffa6d69a68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707964v1_decoy LN:6282 M5:fc00ea543316aed78deefeac0eaf8727 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707965v1_decoy LN:3836 M5:97e94c098f92403b805a0a60f9475542 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707966v1_decoy LN:6486 M5:a977897a46254d42ed9a60e6ed5d9d20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707967v1_decoy LN:15368 M5:220b351d87053eb9b844bc6e6e5182a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707968v1_decoy LN:9572 M5:060b9bb36bc05042b371bfbfc6618e3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707969v1_decoy LN:6413 M5:b25e652840d89c9eef74c04703958f9f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707970v1_decoy LN:4104 M5:d103bed226a1a7b95d98a8d61e39f70d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707971v1_decoy LN:12943 M5:8a88d2df497490f21c8c2b796544f699 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707972v1_decoy LN:4650 M5:93e792bf92036380920f5d06321cafe3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707973v1_decoy LN:3080 M5:127f70f3cf2cf09e8c7a399c471acf75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707974v1_decoy LN:3134 M5:a0447f79285905c6b9f2202fdb83f838 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707975v1_decoy LN:6211 M5:931aa6a0b0412241d93c942acba0c103 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707976v1_decoy LN:1126 M5:c068c96f088fa2ef7cfe6a805c22121a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707977v1_decoy LN:1101 M5:e86b95e84b7662e5e6e509be83db4b41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707978v1_decoy LN:1101 M5:d46f001c034076f4a3687b647a980623 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707979v1_decoy LN:2648 M5:fe4d1437f61ce0406d36a071f4e7293a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707980v1_decoy LN:2973 M5:9c4cb203ab1d6a54a7ed36d116c60853 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707981v1_decoy LN:2520 M5:3ccf197ae78ce07e23eef5e3c31fb576 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707982v1_decoy LN:2318 M5:6e5cf7df1252379ee351fb2386bcc144 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707983v1_decoy LN:2606 M5:64cc9674054ed4f7cdf0701da89de296 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707984v1_decoy LN:2205 M5:e5814756cacf0c02827237dcd24155a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707985v1_decoy LN:2929 M5:6a2895e9dda391590b1db42aea33e330 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707986v1_decoy LN:3869 M5:9640cfef88fffced10f0edaf20dbda2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707987v1_decoy LN:1117 M5:60f8230f4e335416aa92256695cbcf46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707988v1_decoy LN:2960 M5:b0447a959fdacb8b553c3684184cf036 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707989v1_decoy LN:1009 M5:75d4d43433fa532ce2da4a724ee25c42 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707990v1_decoy LN:4048 M5:f0720d93745203f7d155f219ab83b618 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707991v1_decoy LN:2193 M5:fede10b713c7d561d4872feb26040c3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_KN707992v1_decoy LN:1830 M5:1bac13a3ad2592a344e18bf8de117574 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000001v1_decoy LN:25139 M5:9b27ee6060931ae7d17e451b7739e547 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000002v1_decoy LN:18532 M5:9955447b0a6444031f1d68c821fcea22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000003v1_decoy LN:15240 M5:b635cbc1fc3962e93bc1427bc678396e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000004v1_decoy LN:13739 M5:7fa6d2a124f7c9bfeb030b859269f78f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000005v1_decoy LN:11297 M5:44c68451b1d90987e31184e887a24455 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000006v1_decoy LN:10074 M5:ddeeb2059f6f27dee5330cb501a11446 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000007v1_decoy LN:9891 M5:30b47b3ef6b82a46ccfec6573b0c6c10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000008v1_decoy LN:9774 M5:f0bed43412c69b6db572994c9d5d9cd4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000009v1_decoy LN:9727 M5:0cab8d3c0896fb15383dc8aaef1a1f9a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000010v1_decoy LN:9358 M5:aba5595273a0e4398df3a8323523d10c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000011v1_decoy LN:8920 M5:fc623f57c6ad9441d3750c0f51722a0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000012v1_decoy LN:8479 M5:6dca96b0605fddf041e27a9f4beed770 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000013v1_decoy LN:8312 M5:f34a4b1860ec9fbd1acc736a3a174193 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000014v1_decoy LN:8261 M5:6deaa38e9033c12ed1ad7f62191a3e04 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000015v1_decoy LN:8131 M5:c3df1d07395395fb96b81e27ef3dcf61 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000016v1_decoy LN:8051 M5:f732550392ca9c30b42b4c6acd5d9263 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000017v1_decoy LN:7832 M5:03557dc0d8d0509da30549dc16031c51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000018v1_decoy LN:7710 M5:942aca2604695877a0a642916aafd2b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000019v1_decoy LN:7702 M5:c6c2453adbb040df0ea58395995ebae1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000020v1_decoy LN:7479 M5:5b348e642370fdd96c7f2a306318869e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000021v1_decoy LN:7368 M5:0693c472fdc0225c133374a15973bec3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000022v1_decoy LN:7162 M5:6629bbc8b7d3fa10074025773e597c29 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000023v1_decoy LN:7065 M5:6b413d9f61a68a7ce1ea620aebb3be6d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000024v1_decoy LN:7019 M5:14f555be268a0666a71794d0515cd6d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000025v1_decoy LN:6997 M5:470d24c11b0ea5dbd4329912231aba50 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000026v1_decoy LN:6994 M5:1a45d626e4f7add376267b1e77bcc942 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000027v1_decoy LN:6979 M5:6890d5c807ceff3e680405d80c19b990 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000028v1_decoy LN:6797 M5:6cde21bfadb3f954f76aba8775f5b89f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000029v1_decoy LN:6525 M5:091fdeaa7841b60f964f1a9cedc12bd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000030v1_decoy LN:6246 M5:7c23df0bfe1cc7d18d52be126f396cca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000031v1_decoy LN:5926 M5:5555324457dbc04a20ac656a15cce856 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000032v1_decoy LN:5914 M5:67d2d8955ebee73fe7f8f3764e4158f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000033v1_decoy LN:5898 M5:de8113c2b1586e776f28207cd56cec1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000034v1_decoy LN:5879 M5:1c6aa8f142642d8cf57f4f7029a20061 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000035v1_decoy LN:5834 M5:29c393ec5d6804ee5dc06b435ffc02f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000036v1_decoy LN:5743 M5:2f131c59606d7cb8cf6c6c495ce84aa4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000037v1_decoy LN:5577 M5:20bd1281fc32355adf567b4e0ff160a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000038v1_decoy LN:5413 M5:3d2ed60178406278eb02030b7c0cc328 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000039v1_decoy LN:5250 M5:62ebce054cc44d5c32625c44fa1e5ff5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000040v1_decoy LN:5246 M5:7cf464d2ec26fa4cea2bfd25f995969c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000041v1_decoy LN:5118 M5:ad3a1c8041863a52b7fa6d6d56ce0d3b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000042v1_decoy LN:5058 M5:bce45aec79ec4861d8442ec3d738761b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000043v1_decoy LN:4959 M5:f653839b5c54df56a1a2fdd7ceb42db8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000044v1_decoy LN:4853 M5:3505d16e35e056be0892167f5a564661 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000045v1_decoy LN:4828 M5:d43e63bb33a6cb65d5cdd1ca31e94c19 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000046v1_decoy LN:4819 M5:db9c73fa0d581cecaf0175aab4a0fc7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000047v1_decoy LN:4809 M5:1b157b5c5c208470638b188e23b55a59 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000048v1_decoy LN:4710 M5:ad9552980f4f1bf74b72740d14d2f5ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000049v1_decoy LN:4680 M5:5bb4ddca04b26e3afd0bf218f2947a7e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000050v1_decoy LN:4645 M5:6a821c450a9bcf89dfccaa62d46212cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000051v1_decoy LN:4514 M5:bab340cdaa58e6d32ad1138612c84e07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000052v1_decoy LN:4439 M5:3cd292c1df3855202250324272a95b5b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000053v1_decoy LN:4416 M5:73c9659871560313e754f9363053d56c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000054v1_decoy LN:4409 M5:8ab5d2ce8c35201fa585fdf6f4ae670c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000055v1_decoy LN:4392 M5:e2b0cc1cca4b7ed60f85199816696690 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000056v1_decoy LN:4359 M5:ad16965c23d8e07c823a47e9b098f162 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000057v1_decoy LN:4319 M5:3bfb10064835da4e9caf7e8deb1857f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000058v1_decoy LN:4290 M5:947253137780def5c1c0db12039bdeca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000059v1_decoy LN:4242 M5:2a6de19fbea3d44dbf76c71f1778b47e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000060v1_decoy LN:4228 M5:8b1e73bd29fd4c77bb54fb586721b1a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000061v1_decoy LN:4222 M5:8576a97cd4f61cd6455b423f14eaed92 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000062v1_decoy LN:4216 M5:d885db4b883ae34dd53ed184783c2144 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000063v1_decoy LN:4210 M5:1bfa5c8aecb62137e83c3274991deceb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000064v1_decoy LN:4206 M5:48398977c32247e425a6a85f97026636 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000065v1_decoy LN:4102 M5:f7d638a8f28d1c9842128b1225325947 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000066v1_decoy LN:4101 M5:78254b10a1884b61ecbfc72be3ad38d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000067v1_decoy LN:4083 M5:19683f0eb0d378e403a918d10931eb76 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000068v1_decoy LN:3967 M5:2519be1b425e386f50cf02ec88f8f903 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000069v1_decoy LN:3955 M5:7c186eab354093fe3b26a9e757407fca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000070v1_decoy LN:3945 M5:4cf91327091051082a0ff114dd170f21 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000071v1_decoy LN:3930 M5:d97123bfd4ea271ec354c170d89d80d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000072v1_decoy LN:3929 M5:147eecc4607f1ae7fd1dff1c0f98ca25 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000073v1_decoy LN:3924 M5:84bdf303142b233c179ecef62ab58b07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000074v1_decoy LN:3919 M5:648c37f3fa6e7d658440858761213e7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000075v1_decoy LN:3908 M5:36f5d13bc1a3b72a21c0a4b68148d469 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000076v1_decoy LN:3892 M5:052f146ade5330135e5381db9c779f75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000077v1_decoy LN:3890 M5:22c368b726169405e1f364115fc2356a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000078v1_decoy LN:3859 M5:85a6fde3123e4ae2ef854e83b790e55d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000079v1_decoy LN:3846 M5:21dfcbee386bf64a6e7639d212fe4ba0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000080v1_decoy LN:3835 M5:7f8ace0d7314cec254a8ff5addedb0f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000081v1_decoy LN:3830 M5:d40bb66c2d194acad4d612db8d34e1b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000082v1_decoy LN:3828 M5:c6c7826a848a366317f63f5db5dabe87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000083v1_decoy LN:3825 M5:632e853a99e258e0ae002c0c03aa7caa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000084v1_decoy LN:3821 M5:67720b3ea8a4c3be301aa0519627e306 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000085v1_decoy LN:3809 M5:8ee2faad62aedf582f3c33635df47f84 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000086v1_decoy LN:3801 M5:dbd8292192827196a4c3d545bfb9b67c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000087v1_decoy LN:3799 M5:15066eea555ea13dca8b28620db8ae45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000088v1_decoy LN:3737 M5:83437130e836bac50aba2aa79fa9d80c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000089v1_decoy LN:3701 M5:16d790e451ef2c33018834904a04c177 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000090v1_decoy LN:3698 M5:4045cd2ec9d306542cc3804aab489587 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000091v1_decoy LN:3692 M5:3a1e6d05d94debffbe6070b59627d610 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000092v1_decoy LN:3686 M5:f2bcad40026ed9d729d9c31034159300 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000093v1_decoy LN:3677 M5:50536f98dd2e44e8a77c5f9d289ad87c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000094v1_decoy LN:3664 M5:65fd750160fcf7b7944c8e015e46a443 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000095v1_decoy LN:3613 M5:3257e4358f19507804bfac363c932664 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000096v1_decoy LN:3611 M5:d10add8e30105013e264b6feede833e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000097v1_decoy LN:3606 M5:c78427ed0c2856bbf8131bd73e54c3f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000098v1_decoy LN:3584 M5:81a78209d26627e698602af1848e2b03 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000099v1_decoy LN:3581 M5:90e3913247adb7b30059e051bbcefe4c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000100v1_decoy LN:3543 M5:3dd50a694f9e6011fc694fab191cb759 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000101v1_decoy LN:3528 M5:0eefcbd5209d0d32ccf640f76a69f676 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000102v1_decoy LN:3527 M5:0bc37681f399eec3b3b7dcbf22498b37 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000103v1_decoy LN:3496 M5:c04b909a1562f77654eceacd0b8a935f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000104v1_decoy LN:3493 M5:fb84ec004fe99371b9b2d74bfb8570ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000105v1_decoy LN:3484 M5:e07ccdf102c986c26b2bda46045943b2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000106v1_decoy LN:3435 M5:4be97b9bc8d65dffe17f17ef9f801240 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000107v1_decoy LN:3391 M5:0878850e674ca72172a37319b9321acb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000108v1_decoy LN:3374 M5:5b7dd688c7eafb7b418766bdab494a84 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000109v1_decoy LN:3371 M5:fb407b611c6eab3bb1609123cbeb47c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000110v1_decoy LN:3361 M5:4851563bcd9e0f7af3768f20f33bb22a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000111v1_decoy LN:3351 M5:24701a0d266ed39e5bfd3757bbfa218e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000112v1_decoy LN:3340 M5:d10fcebc376b84a0e301d97eff284137 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000113v1_decoy LN:3320 M5:05f2acb99e398b98f272d297727f28d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000114v1_decoy LN:3282 M5:d20adc7c458920890e8c1c56717c60b3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000115v1_decoy LN:3278 M5:9baab7452d6c49d97acd68acc83ab547 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000116v1_decoy LN:3260 M5:b2b6112ec6f9d22a7842f78e37d6a3b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000117v1_decoy LN:3258 M5:edb413acc1f07788b1a55b647c56997c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000118v1_decoy LN:3253 M5:1143d3e269aa80abfae249b7c653814c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000119v1_decoy LN:3247 M5:8d905fa140b8fded3a9560d24a32cfe9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000120v1_decoy LN:3230 M5:8c96125639143835c0e5b29884855e8a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000121v1_decoy LN:3224 M5:6314311a75a1832245fb87dd86655de8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000122v1_decoy LN:3216 M5:80770770768ebb238cb4572d674e1579 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000123v1_decoy LN:3212 M5:63a4964026424edcd64a0e174de5c51a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000124v1_decoy LN:3194 M5:c68922465f39c47a5ebcf4767811e41e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000125v1_decoy LN:3189 M5:9e4ae67a2bea0ce8335cfb82e125cc2c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000126v1_decoy LN:3177 M5:94806d6a47b3781ed8e5004a2c577255 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000127v1_decoy LN:3176 M5:419d834659cd952516de6d9dcae8c3b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000128v1_decoy LN:3173 M5:050df236c1fa3a63c31f33b73308c168 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000129v1_decoy LN:3170 M5:61f6339992ffab5d3091b4a3b3baead8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000130v1_decoy LN:3166 M5:1fc75a1345ce98d6f25ba4d916b5d7f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000131v1_decoy LN:3163 M5:c262387ff17ca2ee56fb368575275676 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000132v1_decoy LN:3143 M5:790557e383cdc852732e3da2c6b6c615 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000133v1_decoy LN:3137 M5:ea2bf77f700236081cf8eb7aa0d9a909 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000134v1_decoy LN:3116 M5:a8ae3ccc9f17c0f0edcacc5079219d51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000135v1_decoy LN:3106 M5:89002d8689e45952391a7984d69df23d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000136v1_decoy LN:3093 M5:2dc162e245d6ff81936bc650004ec8cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000137v1_decoy LN:3079 M5:38068261005ed014603a09dac9438db7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000138v1_decoy LN:3053 M5:bb3a8eab3c8ea2aa961e65ec5e1d91da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000139v1_decoy LN:3051 M5:87d0e9b49ecda7ba160f80012ca40de3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000140v1_decoy LN:3015 M5:303ba1d7f1155342056b491810358e6b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000141v1_decoy LN:3012 M5:c7a07f27a9874d99e33c27c2a2280cd2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000142v1_decoy LN:3009 M5:d88319790f0a837a02a625cfdb6c1971 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000143v1_decoy LN:2997 M5:2b912adb843e4f2d90726c884ca7ab4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000144v1_decoy LN:2997 M5:f6bf68bca6151666f29be31cb16f1c0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000145v1_decoy LN:2983 M5:3473d9484854e710526e0c7ccc80b5bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000146v1_decoy LN:2979 M5:90d61da24b116c2bacb63747666a326b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000147v1_decoy LN:2967 M5:c84a0386ea78dc84323f55723fc42ef1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000148v1_decoy LN:2967 M5:a2b48635d63117baec9f7dbeae644928 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000149v1_decoy LN:2966 M5:0b15201439291bf53043abb6dcdab743 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000150v1_decoy LN:2954 M5:f36be073b0668fe6c56206c0a9bcf742 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000151v1_decoy LN:2952 M5:0aa12dea5cdf7b9dea2d56a71e233dab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000152v1_decoy LN:2934 M5:70eafae03cdb824f956cc77cc9b342ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000153v1_decoy LN:2918 M5:d7711561e6c2a87e3771c3ce9e28ffaa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000154v1_decoy LN:2895 M5:b40089d10ae6fb3b2a90a260a6469229 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000155v1_decoy LN:2887 M5:9be2679cff5e171c42de84446d6fedfa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000156v1_decoy LN:2879 M5:100d6fd294d6998c7928887ba353f441 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000157v1_decoy LN:2878 M5:e081c58ad4b64da9af95ca27932e7ebb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000158v1_decoy LN:2872 M5:0469343a7677dac01c66bf219aa0611d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000159v1_decoy LN:2868 M5:fbc27037c78cd3b3982d7f792147d4ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000160v1_decoy LN:2866 M5:33497edef237132c3e3efe7304d5a685 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000161v1_decoy LN:2865 M5:65371a3805dd2779f5807f1849df8a2f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000162v1_decoy LN:2864 M5:7e9448d377e58534ce56c68cd97278d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000163v1_decoy LN:2859 M5:93dbbd6f23a68673abfe169d241204a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000164v1_decoy LN:2854 M5:c468c0f380889b274a988802cc95f8ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000165v1_decoy LN:2830 M5:bb1fb10e20767215b036dbad18b92018 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000166v1_decoy LN:2828 M5:9f9279b092a64e7648f91091e5112414 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000167v1_decoy LN:2824 M5:774c1242a9fec9c39b231e3a4244c48f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000168v1_decoy LN:2819 M5:5e794be8f02e6190282545c4a587a131 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000169v1_decoy LN:2813 M5:f7e3bb8a09ce56ebcb9638b710cd2ce3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000170v1_decoy LN:2809 M5:8fb1846cc5b62db20428a2bf2bfaded5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000171v1_decoy LN:2802 M5:9bd40f006580d92c066ff544ddc22d8d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000172v1_decoy LN:2791 M5:5f91fa423cf6ea436c30cddd27d2f7b2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000173v1_decoy LN:2783 M5:698dd6fdb96a0f8653015f1bf3406d29 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000174v1_decoy LN:2778 M5:3aae745709a336cef318bd095be7bf93 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000175v1_decoy LN:2777 M5:14ba8c26038f60c5a103e430e7fba5e5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000176v1_decoy LN:2770 M5:467d903833c580a48fa311a7a15c61c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000177v1_decoy LN:2769 M5:beb768213f5d753b7f6a857687fb1c9b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000178v1_decoy LN:2767 M5:2d998199f83204c294ff1e618648e88a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000179v1_decoy LN:2763 M5:9244414120396c3b53b847abfd80bc74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000180v1_decoy LN:2745 M5:8657cb328a0d649107216245ce042ff3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000181v1_decoy LN:2742 M5:9a35552497474ad6e9b35b843c759b60 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000182v1_decoy LN:2736 M5:4fdea3271d6795c56d47fc6d54f11972 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000183v1_decoy LN:2729 M5:5da3445ac176ed7cca1131267e6dfb95 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000184v1_decoy LN:2726 M5:9cc9d79dd54416be6fc2b500d4ca8223 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000185v1_decoy LN:2719 M5:8d898d7f057764e7d11b7121f8cfb366 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000186v1_decoy LN:2715 M5:0bd839a4a62fe7568bf02c2bdf660047 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000187v1_decoy LN:2708 M5:0bcff7297c716264bc76813a0516ad12 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000188v1_decoy LN:2704 M5:0ceefe5f0373922aa642a3180f917c9d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000189v1_decoy LN:2692 M5:a1ed1d3ee4d5cc17aae0d9d0fa0775b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000190v1_decoy LN:2691 M5:43df7d9d35f2d15ff844b2f4564338dd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000191v1_decoy LN:2690 M5:b7809dc0c98369b8235b36dfbd7e2ae3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000192v1_decoy LN:2687 M5:a61002b74d916bbe06d09eb62cdb9144 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000193v1_decoy LN:2677 M5:02cb7169934dce464116e8644638b22d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000194v1_decoy LN:2668 M5:0e691a59ae468d427df06bdb2e34e315 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000195v1_decoy LN:2668 M5:4e1222dfcb9ce80e3a7544e3af791e70 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000196v1_decoy LN:2663 M5:5e4092300f7a24534a4fc2afe6c68b5f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000197v1_decoy LN:2655 M5:8bf68a2085f6903253894b5ac2be0d10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000198v1_decoy LN:2644 M5:035eac523101444b286c852a129bb0f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000199v1_decoy LN:2642 M5:518ada0e1f2b63c0a99a0154b584120d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000200v1_decoy LN:2632 M5:40870ec46cc59053e01def95871a68eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000201v1_decoy LN:2632 M5:af03b437e04ad5f5cc86e2a67adee8ff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000202v1_decoy LN:2628 M5:fa043c52050897ca77e0b475bc77776f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000203v1_decoy LN:2623 M5:0761598e68213207c0d256d5e7e1bcbb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000204v1_decoy LN:2622 M5:502e3af12800de14f86b2cfd3de9bd2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000205v1_decoy LN:2619 M5:74fb4a710fd8bb14104c1cbde54ee247 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000206v1_decoy LN:2605 M5:73c6a5d1feb99a88b122655b4dcdd742 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000207v1_decoy LN:2603 M5:2d1e488c4ee483df72211d2762b1b7cc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000208v1_decoy LN:2601 M5:c72de668308e8c098888b96b543703d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000209v1_decoy LN:2598 M5:33dd6c20ea4712d7d3b200db8f026cbf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000210v1_decoy LN:2597 M5:cd6c72af3776b51a1619b01b5eff8bc8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000211v1_decoy LN:2596 M5:40ba1e4b99e2625ec5a5cc4038fa9a06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000212v1_decoy LN:2594 M5:fe8d3058e4af4e6063be2c2f955b2bfb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000213v1_decoy LN:2586 M5:2ae32263b19fd8541852cb566f82be30 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000214v1_decoy LN:2585 M5:873a6619e50330ccdcebeb56cf56b70a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000215v1_decoy LN:2583 M5:13d7b02826997cd3db01ccfc712000ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000216v1_decoy LN:2578 M5:f891eed2ad267879c435cbc346ebfaec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000217v1_decoy LN:2569 M5:17baf081bd68afecee4fb26591fb7d44 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000218v1_decoy LN:2569 M5:b543771b65b36e55866a9191f68deb0e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000219v1_decoy LN:2551 M5:cd59a6042156743484b01e429d67dcb9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000220v1_decoy LN:2548 M5:822737a44cc33c2db164829922cdb96a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000221v1_decoy LN:2548 M5:cfc1c1a98c1e72f1193cf3bdbd7d6e22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000222v1_decoy LN:2546 M5:4b897ac33360cfc5dda50c492edd5264 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000223v1_decoy LN:2545 M5:3e5f015ea4c3e8e3f4aa413fc8e33ff7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000224v1_decoy LN:2534 M5:8a161ea48715e813fd05aa43c1e4524a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000225v1_decoy LN:2533 M5:fb3804f1f8c9b481a5684e24912cdb2e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000226v1_decoy LN:2522 M5:4a6ae65ea92c112f656b5d39d76a7741 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000227v1_decoy LN:2522 M5:4b56d4e6c02a6e93eace0b35a8e81178 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000228v1_decoy LN:2515 M5:38e333d2fbaeea1196ff289ff007b136 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000229v1_decoy LN:2513 M5:6948ea45d0167313b03bb520dece25ab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000230v1_decoy LN:2507 M5:ae1256f74ac53ab9f03548cd40e2fe8e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000231v1_decoy LN:2504 M5:615e1e7e5b15386539deb06dd4e70457 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000232v1_decoy LN:2497 M5:2601507db41c0111c04735355c122721 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000233v1_decoy LN:2471 M5:204b14540e382d6178cbf75d805ad164 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000234v1_decoy LN:2465 M5:8bf3deb57ae47354c0f07b477420f838 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000235v1_decoy LN:2464 M5:ef6b06a889077e9b0c4ae3b5eeaf21cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000236v1_decoy LN:2459 M5:cf22036ebb20e5c07502642f1d926f55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000237v1_decoy LN:2457 M5:64a6bc4d08e4f9e0d6ba8cce58b7f505 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000238v1_decoy LN:2450 M5:e83868519a491203921eb9f7286a5e98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000239v1_decoy LN:2435 M5:8292756d8d376c15256d12d69951d1ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000240v1_decoy LN:2434 M5:e850a25d74b1a7564101247ce3b1fb7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000241v1_decoy LN:2432 M5:b0696db696f0e45c6909f75d3f17ab87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000242v1_decoy LN:2427 M5:4a78bf09eb53eaefba5d6406f424579a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000243v1_decoy LN:2421 M5:6751a1f3f80e5a43badc5b9a986fa49a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000244v1_decoy LN:2420 M5:b8c086bba1ed8bc4eb0ea05e7031664e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000245v1_decoy LN:2414 M5:4ae74a7c8879b7eadc4ff52f6c247436 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000246v1_decoy LN:2404 M5:cd50b4acbe112fb299328b26c243aad7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000247v1_decoy LN:2403 M5:0a7f0ec7ad3711a0173947d4768d9193 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000248v1_decoy LN:2402 M5:cf164664d17746b996c1b51b7a1b2d1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000249v1_decoy LN:2397 M5:d5f7a437c859d40100bf3a757b38ea7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000250v1_decoy LN:2395 M5:fdf94cbe463685fab85e81430541c656 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000251v1_decoy LN:2394 M5:9e100dfb362640d77d73adb06f63c206 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000252v1_decoy LN:2388 M5:0f0f93cc335a9faecc119d22738a778e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000253v1_decoy LN:2382 M5:af0841d63c2d4ca67c11f8e2f0c0b42b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000254v1_decoy LN:2381 M5:948e4e6ebbce9d82413e86c3c96d684f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000255v1_decoy LN:2380 M5:46be4a58c4b132b788bd04a3c51f0108 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000256v1_decoy LN:2368 M5:722aeea650c487dc132f37322a5b5eb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000257v1_decoy LN:2364 M5:44995a3c81d834bd8bbceda57ee73d51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000258v1_decoy LN:2363 M5:a71f07406eabc3a412250ac21c446ba3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000259v1_decoy LN:2348 M5:99b15b0bac8cbd7497c5ca04a721c657 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000260v1_decoy LN:2339 M5:0768f34e39c5c97c1639fd42b72fec1d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000261v1_decoy LN:2335 M5:974a3d6e7628eaf95db035d99f046379 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000262v1_decoy LN:2332 M5:9a0e708d8a88bfa96a75e98604a273b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000263v1_decoy LN:2331 M5:60412161b03d0c2f2ce5659369ee368e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000264v1_decoy LN:2330 M5:5593063ebd79eae9ac5a5a5ba9095b7e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000265v1_decoy LN:2323 M5:6042dc9c77f44643d41e68ea0cda385f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000266v1_decoy LN:2319 M5:ba867aa03d1617be8f801a89c1530937 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000267v1_decoy LN:2314 M5:a29f663eddf36e84b6cb6faff0f62309 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000268v1_decoy LN:2308 M5:659c8649b3dbe0dd672045a5ed7fa3b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000269v1_decoy LN:2306 M5:e10dadf09b67626744c1c39c1fea5fe4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000270v1_decoy LN:2296 M5:81f921a9ec2bd029889721432b22d4d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000271v1_decoy LN:2287 M5:095a5e8a3ae51b66207f518822bf71a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000272v1_decoy LN:2279 M5:47fde7cc81a14c0c6be4ccbfa3312015 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000273v1_decoy LN:2276 M5:35e9863bbef84bef6634eb206bc66c15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000274v1_decoy LN:2273 M5:9e8590286f229ad55bf921bb4d7236f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000275v1_decoy LN:2262 M5:f184b3c6054e080672a50e932da0aafe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000276v1_decoy LN:2254 M5:06e878f736f352fe167cc033f030b492 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000277v1_decoy LN:2252 M5:af9e9099df9fd3abe01d3ab305c9d65b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000278v1_decoy LN:2245 M5:8f7d8cb82a282dc326d4bd286895492d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000279v1_decoy LN:2239 M5:ae67f16bc2c56baec5093246804b3df5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000280v1_decoy LN:2223 M5:bffd2399ce899d9fa674d826311500f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000281v1_decoy LN:2220 M5:926bf451304bc000be7146acb11c393e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000282v1_decoy LN:2218 M5:adcddfbd560a42060c45d80c751787c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000283v1_decoy LN:2215 M5:706580b59cf8322b639309fc6110d094 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000284v1_decoy LN:2213 M5:7145c3c0ac2cc197f7d0fc0fb18d6c1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000285v1_decoy LN:2203 M5:0c6c215de5d00669ece9d0d461b7b5c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000286v1_decoy LN:2200 M5:a724f88e94788f5e1738a038d67d8d7b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000287v1_decoy LN:2197 M5:fd0cdd36334762ca58e7b82b6ebf802d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000288v1_decoy LN:2194 M5:441ebd8ba7d0673bebf054e6ce9b405e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000289v1_decoy LN:2183 M5:df57267be3acda36409e42dd3c0da306 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000290v1_decoy LN:2179 M5:8bc6beaec162ccebf341b6c46ebaf3fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000291v1_decoy LN:2177 M5:d0d5b33e946654d9b23402cef3f10822 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000292v1_decoy LN:2177 M5:bc189c463b5d9bae5c71afa304910390 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000293v1_decoy LN:2177 M5:c74d19e91cd80fb55bc91184bdc0eac4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000294v1_decoy LN:2168 M5:c828de6bd73d4226b924cbe268ab5d35 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000295v1_decoy LN:2160 M5:33980619d2c675019c4fb26681973f1f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000296v1_decoy LN:2155 M5:40e793695b37b027d9d893448ad20eac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000297v1_decoy LN:2144 M5:55b3946760e337f7fa9358993879d5d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000298v1_decoy LN:2143 M5:5c8fe70b46ce7d299039403820af18c0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000299v1_decoy LN:2136 M5:7a28362f930fcd9a245cc239750dd1d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000300v1_decoy LN:2134 M5:285c47826e725f4442c18898156ac4fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000301v1_decoy LN:2129 M5:b4ad7d61b3d8b5b5f03100792231b056 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000302v1_decoy LN:2128 M5:a6fd1741e7a81251829a88549717a433 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000303v1_decoy LN:2125 M5:c95f162916242e94c057ec50e3f666e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000304v1_decoy LN:2125 M5:cf878bbb13f4cb8f6ad2f6134d4152f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000305v1_decoy LN:2122 M5:ffc29d2524335853c556eac4b936ac47 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000306v1_decoy LN:2111 M5:13ad53d0306dcdc84cfbe5af890ffa7f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000307v1_decoy LN:2106 M5:2705f017f46d301002f0ccf08933080c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000308v1_decoy LN:2094 M5:dc17db769fee486df656f8334528abba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000309v1_decoy LN:2093 M5:61b8e15e0c011168970bf67fa28a893c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000310v1_decoy LN:2088 M5:4a0f2b01b92451953bcb14f94874625a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000311v1_decoy LN:2086 M5:560b32a4aa380573107a95fe8344fcbd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000312v1_decoy LN:2086 M5:f4a368ddba66f5647c78853a2080c7e1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000313v1_decoy LN:2084 M5:90b16478fad5417ff33d03ed3cdb9897 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000314v1_decoy LN:2080 M5:eb02520ddd2b898796bf56257623ff11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000315v1_decoy LN:2079 M5:214af8bfae249648407394750763140c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000316v1_decoy LN:2076 M5:b2439d0b325e4cd30b1869e8e17005f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000317v1_decoy LN:2071 M5:d8e3a93de5b5c8fa58e8785c44e165f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000318v1_decoy LN:2066 M5:03ab659ca0a3d031ed57f5b71c40b7bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000319v1_decoy LN:2061 M5:b7df3a4252d3ed398842dacc1ef5119d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000320v1_decoy LN:2055 M5:3225edb2d4dc98b7b0cfd91afeacdba1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000321v1_decoy LN:2053 M5:d0f66764a23cc8c03b6e0b1b8dcd2aec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000322v1_decoy LN:2040 M5:a53241c8700359fdfc69eff381d88829 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000323v1_decoy LN:2036 M5:8b83d6b87f9d9045336bfe2d4f2d638f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000324v1_decoy LN:2035 M5:641af824694766c85122b263dc1b3649 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000325v1_decoy LN:2034 M5:7f2f9c9fa94f3acab772792c44b4c167 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000326v1_decoy LN:2032 M5:1f32623318d9221ee867209755a538bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000327v1_decoy LN:2029 M5:053ea4692acbe04aa98a6f331417531f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000328v1_decoy LN:2025 M5:930554d67c2f289a7f2bbcce35c88e75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000329v1_decoy LN:2021 M5:b3cda19a888b02b135de2538d4a74936 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000330v1_decoy LN:2018 M5:abfba957d4a6107fa109f1dd5a572de9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000331v1_decoy LN:2015 M5:0ccdc46d98d147f9b6bb6808f723d28c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000332v1_decoy LN:2009 M5:634f88805091cb15be16b1af5e720775 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000333v1_decoy LN:2007 M5:957b5291471bee32d4771292a611cfa9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000334v1_decoy LN:2005 M5:6d2a28c1dcd5377a19f5768b3fa75ad2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000335v1_decoy LN:2003 M5:7fcaa8fce3cf7cda00a42df29b938b6c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000336v1_decoy LN:2001 M5:e28b02249a274e4b98551241e243fcbc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000337v1_decoy LN:2001 M5:4718723746d62e7eed277db09d01884a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000338v1_decoy LN:2000 M5:7071be152f29ebf4ec4cfede2d44d70a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000339v1_decoy LN:1996 M5:21924fac8b9023e37be232641581d24c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000340v1_decoy LN:1992 M5:462035d8f8260ac9d68132b5ac63ceea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000341v1_decoy LN:1985 M5:80c971eec06361d4428c1c731da5d2eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000342v1_decoy LN:1981 M5:6f5d885e4d0a0476e309c004c8d7116c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000343v1_decoy LN:1977 M5:07b50b0be202b0e56fae3ff7b729fcf1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000344v1_decoy LN:1971 M5:ec1eccee490917513ffa0844c16cd648 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000345v1_decoy LN:1968 M5:a44124ee81131e9ba181e4d57343b933 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000346v1_decoy LN:1962 M5:a46b7187d8afab3b6711d2b57dbb649e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000347v1_decoy LN:1961 M5:391b9551b7df1ea41abd51c70c6391ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000348v1_decoy LN:1960 M5:2272602c288691975c385900ff1871d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000349v1_decoy LN:1960 M5:92f15826c95eeb01e4b619c7b646a17a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000350v1_decoy LN:1954 M5:98742b24ea14c25494dc15620ed8d346 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000351v1_decoy LN:1952 M5:5d5e3c8f90d84b8f2e912de53d0250a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000352v1_decoy LN:1947 M5:f5e22c20cf8f7956480ff337ad36be24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000353v1_decoy LN:1944 M5:cd921451a9fcf4ec5d06cb74cee21605 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000354v1_decoy LN:1943 M5:eba8670102e02d674c11f1779a39338e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000355v1_decoy LN:1941 M5:79a20867ab6678a0eb6a1bddd2d467f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000356v1_decoy LN:1937 M5:e17159dcd1117f3bb92bebb710740ba2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000357v1_decoy LN:1934 M5:9266a855ef31e94c5f77d277055f2125 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000358v1_decoy LN:1929 M5:11b07e8db80b814bbcd4814bebafc177 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000359v1_decoy LN:1924 M5:e9df9c6169849c4d69eb8a286f274eab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000360v1_decoy LN:1924 M5:5152172dba1a32a5529708855c21201b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000361v1_decoy LN:1923 M5:22f23fe69e62cdfb4304b679a6916bad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000362v1_decoy LN:1921 M5:b5eaf4b290ab9cf157680d65e640a5a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000363v1_decoy LN:1918 M5:9dedcf6b3a849e4c681f05753c2597d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000364v1_decoy LN:1915 M5:a1c06309d5103a00f8c5cf1847f58856 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000365v1_decoy LN:1915 M5:7a8b1fa154de78062577f95bcbf9cd7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000366v1_decoy LN:1914 M5:6db99643422d9ef62392ced193881137 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000367v1_decoy LN:1912 M5:61c61bda79ca84060bd63c678715b83c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000368v1_decoy LN:1910 M5:64edcf78348cea32c4e946329efd3ff9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000369v1_decoy LN:1907 M5:9266a29087762dab9b2c3f0065838122 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000370v1_decoy LN:1904 M5:f753bc41e46a7b0a9a9c63055a0553fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000371v1_decoy LN:1897 M5:df2a02d92f1a99000001bd1979b387b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000372v1_decoy LN:1891 M5:1664892a75f937da1e68f5c28f0be6f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000373v1_decoy LN:1890 M5:ad597d36bffdf82ff75f444e98be4928 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000374v1_decoy LN:1888 M5:570f07c26f0d742b21502cff22b7d2cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000375v1_decoy LN:1888 M5:a85dc5b82df805bdd2ee38b94de6133c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000376v1_decoy LN:1885 M5:d5cfea6c2a90821c0bac19bb1b81c97b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000377v1_decoy LN:1881 M5:cdcde92ccd92dd4531ee3ccae640577b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000378v1_decoy LN:1879 M5:fa3996c5e3cb86b5904ad5ab423f06ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000379v1_decoy LN:1877 M5:4659566201687f420c913a424ff09d62 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000380v1_decoy LN:1876 M5:e619de3bcb3f53147ec25a74324350be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000381v1_decoy LN:1876 M5:7d297256bbb1ec6cdc08e447670e69d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000382v1_decoy LN:1874 M5:bd4b0fff3b0f9b3c27bc270d80d2d91f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000383v1_decoy LN:1872 M5:d337922792c71f4bb3ec1c522519d7cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000384v1_decoy LN:1869 M5:95a4dae294e133dca09afd04559a3d82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000385v1_decoy LN:1866 M5:38b2fbf522a772de9e5971e2c248d773 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000386v1_decoy LN:1865 M5:a2b125ab6d06ffdfbfde0a2899a63d38 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000387v1_decoy LN:1865 M5:eeb193e267c0aad102b5a8c55b899094 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000388v1_decoy LN:1865 M5:a21001e6acebfcf03c2f187db59bca58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000389v1_decoy LN:1862 M5:e1aa83bd329fed4cea1a31c2b26605cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000390v1_decoy LN:1862 M5:e80fe8b2857e4e57c40cff1a604ca905 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000391v1_decoy LN:1859 M5:e85c5d8da9312ccb56a4ecb47e7cb397 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000392v1_decoy LN:1856 M5:d1448f2aeede93e71be4fa57f641c1ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000393v1_decoy LN:1856 M5:dcbc9c61d537f450dd4fae3b11f71d19 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000394v1_decoy LN:1854 M5:a427d47a812c6fe6c9d26190b4b83f7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000395v1_decoy LN:1850 M5:876c26621463a5218a5953d9be4d0810 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000396v1_decoy LN:1849 M5:4797d275b03add54ab91c5b8c318a7e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000397v1_decoy LN:1849 M5:707d492cfa678968ded0e2306392504a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000398v1_decoy LN:1847 M5:06eb26d8459f3e43735ded45aa07a2e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000399v1_decoy LN:1839 M5:1ec1c61f264cc1ec9596dab78ea77adc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000400v1_decoy LN:1834 M5:c268a5b922576efd86cb97619df6fb55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000401v1_decoy LN:1821 M5:37647f03de6c012f655cbffbfbe570b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000402v1_decoy LN:1815 M5:edf2017fafb77c03c7f392ec8489ffae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000403v1_decoy LN:1811 M5:60131480b353a90b21d6a47f52a7d1eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000404v1_decoy LN:1808 M5:b4009abb588476a2593dd27cef872705 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000405v1_decoy LN:1808 M5:ef9e792232f15cf2b18c4d4a7ce99253 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000406v1_decoy LN:1807 M5:09de8b5234448cff4c1e3defd30ee794 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000407v1_decoy LN:1807 M5:212f6e4df329ece3ab38c483cdf02535 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000408v1_decoy LN:1802 M5:291bcca126e0b971eaa6fde409109815 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000409v1_decoy LN:1801 M5:12ba0451f91998e31fd25003c892102b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000410v1_decoy LN:1800 M5:d47cda8c33b62a63e40f02664d4811d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000411v1_decoy LN:1795 M5:7f941c23c25bebc1937eae96aa5e98d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000412v1_decoy LN:1794 M5:1354d0a5f0d586b01f493802f991dd7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000413v1_decoy LN:1792 M5:7b03b07cb1cf327205fb693d3ace8a42 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000414v1_decoy LN:1788 M5:36d568b75f9e3cf031e037043ac02384 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000415v1_decoy LN:1786 M5:393fd0366a1270f611866e1fb5af3cc1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000416v1_decoy LN:1782 M5:2652e35a4315660b304c6b7522e5f7d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000417v1_decoy LN:1782 M5:c7c0bd282280cb9eb969940cc3b68acf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000418v1_decoy LN:1781 M5:5a9a78f89d320fbabd4693bd232704a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000419v1_decoy LN:1781 M5:75304d27b61d40a759bd36190202926a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000420v1_decoy LN:1779 M5:95cf227ee90e6dced27e307b52d240fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000421v1_decoy LN:1777 M5:c1fd358a71fe269cee210b27840c272e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000422v1_decoy LN:1764 M5:5db13804da88f18750e478f6682b83a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000423v1_decoy LN:1762 M5:bcf551251490bd2c7ab5a99232bbbcf0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000424v1_decoy LN:1755 M5:c8de78702f0a0471f26cfe586a8d9263 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000425v1_decoy LN:1749 M5:9c843f2e59ba4aeed3517c0aa36ed8c0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000426v1_decoy LN:1747 M5:431f95d76dc16b369e6765e38ae92ee1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000427v1_decoy LN:1746 M5:1641a71638dd63211280e6bef22e6a4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000428v1_decoy LN:1745 M5:98de780057460a078034b25725bad406 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000429v1_decoy LN:1744 M5:16ceb3de9929ccc268d4aeacb8f20439 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000430v1_decoy LN:1742 M5:6c61a88798f9fe78567c879793385c29 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000431v1_decoy LN:1740 M5:e2ecdbd050ed1413eb2a07f7d9ebc8c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000432v1_decoy LN:1740 M5:7f1d544cca143be6dd353cf3efb80028 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000433v1_decoy LN:1736 M5:18493fcfd6ea161acd237867aa7337cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000434v1_decoy LN:1735 M5:afe0292acad0a1460fbad00971606090 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000435v1_decoy LN:1732 M5:5bba5da58b43755bdc14ab286a8a5cee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000436v1_decoy LN:1732 M5:680818b10726c68c5917f48456528d0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000437v1_decoy LN:1730 M5:836c13c8ad60355227f91a1b5308ea33 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000438v1_decoy LN:1727 M5:f5fb0c58f9b4fa60b1eeccee56f0cd56 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000439v1_decoy LN:1722 M5:9a47086cf6334392302ea1713fb68c57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000440v1_decoy LN:1718 M5:dcc0114d2cbd5f165da52b4610aeb6b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000441v1_decoy LN:1716 M5:59cfde6a838acc68c55e5a781f1308fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000442v1_decoy LN:1710 M5:cf384ae5b546344ace5aa4d662c6ac0a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000443v1_decoy LN:1708 M5:4df6e9cabebe481359ac95881b277f74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000444v1_decoy LN:1707 M5:971971b7547432d52662e516989b1596 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000445v1_decoy LN:1706 M5:9c926b3b414cdf578a7af119dc3d32bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000446v1_decoy LN:1705 M5:91d17f22298838daf727563421004b33 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000447v1_decoy LN:1704 M5:64c4b3b7b16fb06e268d70c576c18be7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000448v1_decoy LN:1699 M5:e50192f7290b158ee82f04623e78180f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000449v1_decoy LN:1698 M5:938f5fb6e35906afc6601c756e85684e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000450v1_decoy LN:1697 M5:670cd517d0cef208acfe5f83f4f08e40 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000451v1_decoy LN:1697 M5:29b287f217fd519ac38852bf9f3b89cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000452v1_decoy LN:1695 M5:42ba5d9b4331876409b5509b694d32f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000453v1_decoy LN:1695 M5:a98d7b845be68d05f42b70b19d87ecbf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000454v1_decoy LN:1693 M5:bda60eb625bdf4d5f8b0cb6ccb975c1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000455v1_decoy LN:1687 M5:27bc09a272621c3414cbe34cd809347d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000456v1_decoy LN:1686 M5:6f0aa7beab754a13ff566057d0641ae0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000457v1_decoy LN:1680 M5:8cba844ac8cc6321dba7652815bbd94d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000458v1_decoy LN:1679 M5:e2f908a05a86ddbda11acd668b7487e6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000459v1_decoy LN:1679 M5:5a0c260602a6ab3fa76f49157966cf4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000460v1_decoy LN:1678 M5:54a34fdfb08cb07c1f51c8be679c6339 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000461v1_decoy LN:1674 M5:ced48cb3354e7f24d532fefc85a96319 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000462v1_decoy LN:1674 M5:4467091f25a983fc0f7d9d88bd351993 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000463v1_decoy LN:1671 M5:7cbbe8ba7b64b120b467c953851a7eb4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000464v1_decoy LN:1669 M5:a3cdd6c546af035a3c9830a67c0bb056 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000465v1_decoy LN:1665 M5:1e245fa1bc113bab6305d0fc3cad2112 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000466v1_decoy LN:1663 M5:b1a1b5888971729811d7fcf9b06665e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000467v1_decoy LN:1657 M5:8a989e9803993317d80157778c71da54 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000468v1_decoy LN:1653 M5:bc73c770766d2b8deb017d234f5d7387 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000469v1_decoy LN:1652 M5:9559e20eaa71db947eb9645779c2c2e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000470v1_decoy LN:1650 M5:60a6f98d6f706aa9554c7b6ed3de2716 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000471v1_decoy LN:1649 M5:ce3eddf63b3aea572dd8e0b762b90829 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000472v1_decoy LN:1649 M5:aeab25a6fcaf97cdcb319dff1550df0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000473v1_decoy LN:1640 M5:7a4f3084ac2ed72ea4f5e60260c77948 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000474v1_decoy LN:1638 M5:5c4753a4d69db9ff02e858c8ddbb8911 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000475v1_decoy LN:1636 M5:8970166c6ade7a2f8106159d5ee5c857 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000476v1_decoy LN:1632 M5:67037d44662f2487a2e39e76ea3fef94 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000477v1_decoy LN:1631 M5:3455484eacb9d4eaa7ed63b96b087c85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000478v1_decoy LN:1630 M5:c20fcc553e42922058f9f0e839f527ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000479v1_decoy LN:1627 M5:cf02dff4c16e81e47d1cc5ffdbf1b4ae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000480v1_decoy LN:1624 M5:93392bdc58b6376d5da876bae65be9e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000481v1_decoy LN:1617 M5:e8add50c6a06030a1dca84968a89d4b2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000482v1_decoy LN:1616 M5:6d496ad94b5cd40c72f5362e446e22c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000483v1_decoy LN:1615 M5:bcc4bc611bdb483f2f925df072d50a41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000484v1_decoy LN:1611 M5:19f0a56814e718041b51733abc55a44c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000485v1_decoy LN:1611 M5:771186a23da9b9c2081773c5a6d73812 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000486v1_decoy LN:1606 M5:f6dc33d2578fb0ce6a560d5ee7b9b5cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000487v1_decoy LN:1605 M5:440c7d94624635647d771ce7f3833416 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000488v1_decoy LN:1605 M5:7e6682db43f2445cfc3b18ddecb786c0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000489v1_decoy LN:1600 M5:1b3265b9aa7cae61d843ae0b09e2f3a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000490v1_decoy LN:1598 M5:8ef2cd8ef0749e5d753b552c1f2fc3d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000491v1_decoy LN:1598 M5:9bfcb4215c94e812ae4ead4fc804a63f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000492v1_decoy LN:1597 M5:8c06a1c635581dd76469e431bda358b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000493v1_decoy LN:1596 M5:9880ad7b13fdc3af8d8c81e3adf8ba43 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000494v1_decoy LN:1595 M5:ec1ac1310cc7d4432ced7724829cbdba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000495v1_decoy LN:1592 M5:f2d436516525e67ce3aa61ec5273dcb5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000496v1_decoy LN:1589 M5:201a121b6775880be8593d2a9cef12cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000497v1_decoy LN:1585 M5:491aed38b1a89a77be7b2d1d30bd4fa4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000498v1_decoy LN:1579 M5:2e63f3a1cf01e023c443b4478459db64 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000499v1_decoy LN:1578 M5:f9223dc0eaf125b8226c386bbca18647 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000500v1_decoy LN:1577 M5:3c9bc60202ddedaa3894d11a96f5429b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000501v1_decoy LN:1577 M5:c367b54edc2b83cb15946ac3c975ccfd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000502v1_decoy LN:1577 M5:d7d24f1188f1d67db7215729a5c5b8d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000503v1_decoy LN:1576 M5:229d2aa7ab158e9b4bad158972029e55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000504v1_decoy LN:1575 M5:c8f35742cdde94078de3c3d84c130c42 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000505v1_decoy LN:1574 M5:d0367db77d5a6bff49f54d4acd3d01e1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000506v1_decoy LN:1572 M5:06b66d904a7902fb764a3e661b9c8bea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000507v1_decoy LN:1571 M5:e3187302e0771a467d4e7d2b31669e7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000508v1_decoy LN:1563 M5:78aa7cd4461790084af50b1b80e1838b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000509v1_decoy LN:1561 M5:abcdf15d561b447b0bdedb366fb77d72 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000510v1_decoy LN:1561 M5:f9a7e72f7e382fa59a1d2c0c4d6edc53 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000511v1_decoy LN:1560 M5:bbfe4f1e02f2fdcc9d20e65e8d3da35d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000512v1_decoy LN:1560 M5:538f38f8d5e6495a85b6bfecf487ecc6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000513v1_decoy LN:1554 M5:8ae2f8b5f30a6c1762de005819965ec7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000514v1_decoy LN:1552 M5:392885482ea57d0e3f1311c3be95c89b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000515v1_decoy LN:1548 M5:1310bfff4743d36d7f8153b6663f2249 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000516v1_decoy LN:1546 M5:629769b2550c48e2a73115da95946951 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000517v1_decoy LN:1541 M5:76f0c1d1779803feb107f253ea555df7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000518v1_decoy LN:1536 M5:686a5b0fc214ac36df8da7605ffae4ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000519v1_decoy LN:1533 M5:e2bf8fa01d69d0ec7c7e1f6d040912c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000520v1_decoy LN:1532 M5:0c23895ba0df7ad7ea8f2b58313a0cdf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000521v1_decoy LN:1532 M5:c9515245780a66ef482674755d38c592 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000522v1_decoy LN:1530 M5:5caa4ec6f260d2f960770b368909d78c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000523v1_decoy LN:1527 M5:b05daa30b4f2c9167aac9fde662de4c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000524v1_decoy LN:1526 M5:cfcd1e358a225fc7c2354b1bad52cccc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000525v1_decoy LN:1524 M5:08ec33c06085eb399a1e56f43d1d222e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000526v1_decoy LN:1523 M5:74f16c21c5a2372050d2f61595d52eea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000527v1_decoy LN:1523 M5:e7cf8c07771c8fa37bf4b6fbca555113 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000528v1_decoy LN:1522 M5:8f754d63b65c3c7a1e9a1ad5e439d2d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000529v1_decoy LN:1522 M5:51530101930cfdb66060d0686c4fed16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000530v1_decoy LN:1519 M5:dced229b77a1ea722aed13b4eb4e5b79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000531v1_decoy LN:1513 M5:dc49162a2d904001541bef4c7f7cd54f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000532v1_decoy LN:1508 M5:c183c701388b1a11f3cbf31ba2d84f41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000533v1_decoy LN:1508 M5:0d9b9c8ca5a3b58a13369e0a097f25cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000534v1_decoy LN:1505 M5:4e806d8438bd8512d22e24a026640d66 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000535v1_decoy LN:1503 M5:0b9c580f3d47c52b031cb00b9741c1a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000536v1_decoy LN:1496 M5:ebce7ee144631f93b7001740bff968ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000537v1_decoy LN:1491 M5:191de45ffda752707ef5b22c780675b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000538v1_decoy LN:1490 M5:2b8af44ac46088badbdffea0c0c3d368 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000539v1_decoy LN:1490 M5:378e070f5556e26fd1e89f1f21e2c35a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000540v1_decoy LN:1487 M5:7c84677ee13bffaac6c5c623f317619f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000541v1_decoy LN:1486 M5:501226e799037a04ea73a3be5bde460b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000542v1_decoy LN:1485 M5:8ec76a3f62b296d50ccff9ce4a9aa072 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000543v1_decoy LN:1484 M5:34e4860b41e4db3fe4ec16fdba4cb85a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000544v1_decoy LN:1483 M5:ca6b3496195a36f5f1a5a1de26bd9655 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000545v1_decoy LN:1479 M5:902f04a9f4b960cef80039434d00e63f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000546v1_decoy LN:1479 M5:16692f78928924e255aeb5b72a97ab21 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000547v1_decoy LN:1476 M5:e765f1919b45a51913ed7d2f4c9a7491 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000548v1_decoy LN:1475 M5:fa46ec2fd14b9d99852d4d30568593ff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000549v1_decoy LN:1472 M5:d03e8e717a38f0178037af0ca86aafb4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000550v1_decoy LN:1469 M5:8ee65cdf657a82a381d7cb6caa4f3f5d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000551v1_decoy LN:1468 M5:bc6f74a7c8ffab037e3eab5ea88037a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000552v1_decoy LN:1467 M5:7d64a0f8c6675f07e9dec9b70a0afdd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000553v1_decoy LN:1465 M5:2e3b43fdc0d1784a2518dfe255384519 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000554v1_decoy LN:1464 M5:df762923ca57a0113032d663d3c97bea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000555v1_decoy LN:1463 M5:11aada1e695fe5177d9540b02a2d432b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000556v1_decoy LN:1463 M5:d3ab1903d41ac470157b0c4d4ed61933 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000557v1_decoy LN:1459 M5:bbadf4ad4f9a1ee8da95612868cfb018 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000558v1_decoy LN:1459 M5:bcd6dabc3d1399c379f3adf1cdeae332 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000559v1_decoy LN:1458 M5:1781d0b5245fdca60eb63e40b293194d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000560v1_decoy LN:1458 M5:46a7c1c5c2e823abe406cf459179bfde UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000561v1_decoy LN:1454 M5:a7a571b77c5a922ca842288fc6734696 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000562v1_decoy LN:1449 M5:cc0f411395c55cc332ad0889e75eca80 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000563v1_decoy LN:1449 M5:184e7321ea1fcdc47df967c7c5d879f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000564v1_decoy LN:1448 M5:9cc22c1be7f706f6789fc994e46ddc95 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000565v1_decoy LN:1446 M5:2be7af38d4e11bbf45397add5e8b29bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000566v1_decoy LN:1442 M5:7badb67269751abbac8f46b5704299b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000567v1_decoy LN:1441 M5:ee8162f4c41a8b15758b34b01d87e2d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000568v1_decoy LN:1440 M5:d4b0ebe0d4c6d4d270b5a5b7d1a5d7ee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000569v1_decoy LN:1439 M5:ec8779a6ca200bbe41de6dd3630da10e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000570v1_decoy LN:1437 M5:3d29e003ab19ba610718173d23d26b37 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000571v1_decoy LN:1436 M5:a7261a67e8f0dd3de7bb37e12ed74c3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000572v1_decoy LN:1429 M5:d9b8501a054b0ba4229f7ae1e74bf2c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000573v1_decoy LN:1429 M5:ddc6caafb94fef7d6209cdff64795226 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000574v1_decoy LN:1427 M5:9aca197fbaf212c69feea234780489a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000575v1_decoy LN:1426 M5:1e4c043b8c89e6c193c519db2531e6b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000576v1_decoy LN:1425 M5:846748418b1797e21e540d92fd794495 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000577v1_decoy LN:1424 M5:4fd0c37d4a586150a9301f2009c5c1e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000578v1_decoy LN:1424 M5:54ac14d019ced5f6bfe22948074afa9e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000579v1_decoy LN:1423 M5:3fdf802f805e746ac56f30d6d1a68708 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000580v1_decoy LN:1423 M5:7237c88f6ea636176c0287d0432a9732 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000581v1_decoy LN:1423 M5:e9eaf98ae51879086e24b98bea88df0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000582v1_decoy LN:1414 M5:34272cd5beb57237fb7a193f798cc2d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000583v1_decoy LN:1414 M5:9060d1ca5fe447374bc2a1e69f4e3224 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000584v1_decoy LN:1413 M5:3298b158d1b1f933a0e2a292a1529e10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000585v1_decoy LN:1413 M5:59c1a460224d4414afdea6e15bd8b2e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000586v1_decoy LN:1410 M5:64228ed4ae27b3994019d9e0038f55a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000587v1_decoy LN:1409 M5:4d845a6ec097602753d860feddf23e58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000588v1_decoy LN:1409 M5:a000b425fd931ce6015497afb22efd88 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000589v1_decoy LN:1406 M5:0c059afc9f69018ba9da0b23aa8b9b75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000590v1_decoy LN:1405 M5:0c586f3eb4883fc1918171f471f5e5f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000591v1_decoy LN:1405 M5:cd72088875313abb75d2a891e35b7d4d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000592v1_decoy LN:1404 M5:abf874737ff5cfa8f718112f32beb985 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000593v1_decoy LN:1404 M5:eb8f0a5cc692b80655ef58e2547a4cec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000594v1_decoy LN:1402 M5:6ee2ff095b70b42cd9c9b4e0efabca4c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000595v1_decoy LN:1402 M5:f4d8da8a43b238c8e0646f5f0b52f89c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000596v1_decoy LN:1402 M5:8c7872a37fea0f290dc1bc82d975b894 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000597v1_decoy LN:1402 M5:d0d9fdcbf88b68b1dec6f806c72fed32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000598v1_decoy LN:1400 M5:6634e5294042b88996256c18dfedb84d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000599v1_decoy LN:1398 M5:fd8634e8ef145e4987e4520f150aa3f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000600v1_decoy LN:1396 M5:25350d57a6af0ff5c2c4c9c49b44ce71 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000601v1_decoy LN:1395 M5:6aedeed255c7942bf7499cf1627f8cbb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000602v1_decoy LN:1394 M5:535e79d7dc446b1c3d57759eee6e4f79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000603v1_decoy LN:1393 M5:300693e040f4152f622506cde18980f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000604v1_decoy LN:1391 M5:8a946b3527d782026e0dc90559017170 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000605v1_decoy LN:1389 M5:7a9e96381bae2eb52e9e340616f3d40f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000606v1_decoy LN:1389 M5:b6ee1b27e065ac10608cc2a75f23b552 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000607v1_decoy LN:1388 M5:ff5370f5970d37597e705a08fca533b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000608v1_decoy LN:1387 M5:445d8c03bad3cc26e6adeb0047d18078 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000609v1_decoy LN:1384 M5:e885e52d68b2de3b4a858205e3e642ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000610v1_decoy LN:1381 M5:56708189f55fba9d8406fbd9eee51424 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000611v1_decoy LN:1381 M5:c7791c86ba8e059389aaa3919f1ed24b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000612v1_decoy LN:1379 M5:040a6029ad56ae20245dd1757c313f19 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000613v1_decoy LN:1377 M5:cc1f3e1b6fbcf7cf33ba399c84ff0fd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000614v1_decoy LN:1376 M5:73782073ee88b30e5184f8c58b162395 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000615v1_decoy LN:1376 M5:b49adf2ce996df0a067aa5afccc4d593 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000616v1_decoy LN:1375 M5:ebef890f7fb5122bf631a5489d6d46a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000617v1_decoy LN:1374 M5:d165c7fd94b756243531a20cd030e7f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000618v1_decoy LN:1372 M5:99bb1f26eea260757df2246652e20ce0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000619v1_decoy LN:1371 M5:fdc45233952efe3a4444e4fc118e9bd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000620v1_decoy LN:1370 M5:023468d3dca8b3726de68eb85c7d2301 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000621v1_decoy LN:1370 M5:fb5072d4c89ac94df7141cacd9701034 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000622v1_decoy LN:1366 M5:5aee91a1f323fd56c65017a1878fae17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000623v1_decoy LN:1363 M5:6b6ecc4a02c37b540c86cd51299d13da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000624v1_decoy LN:1360 M5:06cd979553eb7045f14feac8e6f2e2bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000625v1_decoy LN:1356 M5:d4cf98566c01e8715f3f2d3883ee0960 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000626v1_decoy LN:1355 M5:1dfb4921c6f707d06505f67e59849603 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000627v1_decoy LN:1355 M5:0d374e2ae6601c90f80dad8772daeec6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000628v1_decoy LN:1352 M5:474bb6fe17aa82dbe052602181e2c342 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000629v1_decoy LN:1345 M5:be38a1cda1a6e1ae5e323c69f41169ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000630v1_decoy LN:1344 M5:5f167804d39afced5ca6f11bdfc4e79e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000631v1_decoy LN:1344 M5:a8c1265cc335c37e42363c32f7004283 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000632v1_decoy LN:1342 M5:7aad5f98492b8fca78e060b6be39462a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000633v1_decoy LN:1342 M5:f3ffd07c1b2dd961b6182008cc89dca9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000634v1_decoy LN:1336 M5:64e80f53b6b20fe69e1f570c4bf46bdf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000635v1_decoy LN:1334 M5:49441fe73d5a6c1424175db5c183d5f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000636v1_decoy LN:1334 M5:7d75d91acde1b12b627eec4355428b3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000637v1_decoy LN:1333 M5:e4ece619ebb5ab86de6fab33b38d5481 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000638v1_decoy LN:1332 M5:7135701f246e43df19f5aba147d6ffe4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000639v1_decoy LN:1328 M5:fbb7b0f582ebca6f3cef730730d1ba0a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000640v1_decoy LN:1328 M5:62be463086e26b1df7d57735080ccdad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000641v1_decoy LN:1328 M5:14eb9b729a7e8e201661d1337b6c986d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000642v1_decoy LN:1327 M5:7758f1665792870c0c087b3c3dfa6f6f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000643v1_decoy LN:1325 M5:4e1088e1aa76be60fab929e87bddd522 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000644v1_decoy LN:1322 M5:af3b1f03c49d83adafa3d8f2d6874c24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000645v1_decoy LN:1320 M5:446dcf62ddc2538c64ec6aa731ea150b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000646v1_decoy LN:1319 M5:56748fdfa4712830c7af4c68f4759228 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000647v1_decoy LN:1318 M5:8eaa0a6e0ed839c74aab55d260c6fc46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000648v1_decoy LN:1315 M5:56712d4e2ff952bae0ab0ddd610f253d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000649v1_decoy LN:1314 M5:d3e12fcc460574d688e5bae5d2442b1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000650v1_decoy LN:1313 M5:b547545a4b0358b765cecfc76a24f5c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000651v1_decoy LN:1313 M5:6b644257e459efb9331cbf248eb360a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000652v1_decoy LN:1312 M5:49650bf9d34993f3734ff8be22a21d8c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000653v1_decoy LN:1310 M5:a4ce42eeaf93dc12fd2c1d548ecb8ec1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000654v1_decoy LN:1309 M5:b6aa3601c472a96642590f2556fd60f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000655v1_decoy LN:1309 M5:86d4783a9574d129cdf2d96b81899a69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000656v1_decoy LN:1307 M5:f7f361b3f660b392044fad24ffae29fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000657v1_decoy LN:1307 M5:754ff5c46ace6aa00b6225c1395948bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000658v1_decoy LN:1305 M5:93cb281ea84aef36a85cb9950604dd0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000659v1_decoy LN:1304 M5:12d269dc2ce62735236ae94e1f20075b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000660v1_decoy LN:1303 M5:48fbe06e5f32a0378e9bb9f94280b087 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000661v1_decoy LN:1302 M5:21449d3d68824fc9c13706be068c9b2d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000662v1_decoy LN:1302 M5:8b61707178e6e8435c171f9af9103412 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000663v1_decoy LN:1301 M5:00401a0950f66bdb8079810e7cd7ea17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000664v1_decoy LN:1301 M5:da9b6adf8123090f1132c9b33c3aa8e1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000665v1_decoy LN:1300 M5:a6e3addcf5e77b6d35b918c3d4b07184 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000666v1_decoy LN:1299 M5:74108c185dba89f41809a3192d33baec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000667v1_decoy LN:1297 M5:4aa3c2f67ac1c8f495575e8560808f7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000668v1_decoy LN:1295 M5:c0e3f662ea9b92faded08da52ec79ba1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000669v1_decoy LN:1294 M5:acb9567104820929d7f17199b614c243 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000670v1_decoy LN:1293 M5:d58180a24710ca20cd90857426fba9cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000671v1_decoy LN:1291 M5:0021de79f69c7e6f7be872209251d8a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000672v1_decoy LN:1291 M5:ec57c06e1967b151af9716613687bf06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000673v1_decoy LN:1289 M5:9e48e5eb17a1e58951e1b652dd9f44d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000674v1_decoy LN:1288 M5:93d19765c9dd00919c6cfa7d93347c46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000675v1_decoy LN:1288 M5:71235a1d58334d223edcaa90fefacfdd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000676v1_decoy LN:1287 M5:a76f2f42317a6ab2cb892a72174adb45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000677v1_decoy LN:1287 M5:f334340b46a994fa73de757843dce680 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000678v1_decoy LN:1287 M5:e2d24061f1801ed1293ec0b863363b3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000679v1_decoy LN:1286 M5:23647de37c2ff39cd67aaef8a5580814 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000680v1_decoy LN:1283 M5:1dbcdc4ac713f81604583fa6b6106bb4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000681v1_decoy LN:1281 M5:bb3d85c707984bea66a348264a710aa3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000682v1_decoy LN:1277 M5:5c2e53fdc0f5cd26c68ab66f14416b3b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000683v1_decoy LN:1274 M5:f1d20984b2bcf2b2b0e005c54fff514a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000684v1_decoy LN:1270 M5:0e776546d49756c788dae25d1787fb00 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000685v1_decoy LN:1267 M5:e52038740e15a18dd9b464342630faed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000686v1_decoy LN:1266 M5:9b437657983b2138524382ca0130b93b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000687v1_decoy LN:1260 M5:0a5f065c6de74f1ae3424582269c48da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000688v1_decoy LN:1259 M5:78788ef8ba3807f9a105d4a9a55a3c65 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000689v1_decoy LN:1258 M5:4860c125f57848f7afe40d583f7477ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000690v1_decoy LN:1258 M5:2713e855b2f8f1734239f0523b6200ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000691v1_decoy LN:1258 M5:619c058b99f936b291f949550793c9f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000692v1_decoy LN:1256 M5:c21ff94783721fb59f3db81a80c1e0d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000693v1_decoy LN:1255 M5:8c83cfffa25879e35ab28858fa33d70a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000694v1_decoy LN:1254 M5:b0caa977dfdfb73b5e1a58b423ff4a8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000695v1_decoy LN:1254 M5:e2dd86e353ba1cebb9ee6ed4e35b54f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000696v1_decoy LN:1253 M5:5036e5442498d2a7fdb0f573f258cd60 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000697v1_decoy LN:1250 M5:a082ce0af37351135354a3448f4e1e21 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000698v1_decoy LN:1249 M5:d47b3d78169ea825e882ac16b3117146 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000699v1_decoy LN:1248 M5:208d107183937e7c221f38693342e39d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000700v1_decoy LN:1248 M5:0f92b053b9f7b2d596ce45e73486fe32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000701v1_decoy LN:1247 M5:38be4b2a0f1e5aa9c09b662eee046034 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000702v1_decoy LN:1242 M5:05cde0965d9330b1048d4d3c3d3d700c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000703v1_decoy LN:1242 M5:0861806c0301513ad259fe070d39e0dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000704v1_decoy LN:1241 M5:364bee56428db3836e130cf7a18b713d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000705v1_decoy LN:1241 M5:854cf124e49f41012ddbad98fa6676d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000706v1_decoy LN:1241 M5:1f8ca7e72524d4e37948801d7b5a8afe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000707v1_decoy LN:1239 M5:f24cb34121b984ca68855edd34574629 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000708v1_decoy LN:1238 M5:d209df786f3b1d1ad941874631548588 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000709v1_decoy LN:1237 M5:b4a98205bda995826a33cefc257ca695 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000710v1_decoy LN:1236 M5:e889a8feacb30e59ab4f4b36b7d9c1e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000711v1_decoy LN:1235 M5:1d00a257b98ad31640bb5ba492b84179 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000712v1_decoy LN:1234 M5:33721b9972767397365deed771dbf073 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000713v1_decoy LN:1234 M5:036d24e0ed6b0b50f1cfbd3f587c4cc2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000714v1_decoy LN:1234 M5:a7ddd63607d708a84ae2b88906989e89 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000715v1_decoy LN:1233 M5:9eac1d4d910f6c899ce66d778f2f1151 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000716v1_decoy LN:1232 M5:0b8643f1a24c79b3ecdd1a67db0cc86a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000717v1_decoy LN:1232 M5:c5a5a989ac7252d8392936662ea77847 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000718v1_decoy LN:1231 M5:ac3030bfebae64338eb50915e6c8bd90 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000719v1_decoy LN:1230 M5:57901862b298a5add08f5cca114254dd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000720v1_decoy LN:1228 M5:425fcf156dc340ca958e76e678b5b1e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000721v1_decoy LN:1227 M5:8202dba5ecf55e01c2eaa23654a65b28 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000722v1_decoy LN:1227 M5:84410313c42ed3e74a0db6afd68aea27 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000723v1_decoy LN:1226 M5:abbfddc0795df55c6e15788256752132 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000724v1_decoy LN:1224 M5:a62c3a744a6da312ddb499103c0e5dd1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000725v1_decoy LN:1224 M5:8de8180c87074519ebeedefce23a5bd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000726v1_decoy LN:1220 M5:f04fd8bfac48f74f1ba90d5f1730840b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000727v1_decoy LN:1220 M5:f0dfe089c3f7b049c98dc63e934ff352 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000728v1_decoy LN:1219 M5:e7c7be67e8022eceeb1748e1bc896244 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000729v1_decoy LN:1217 M5:d54b84b201adc65a8f6c321c0a9b82b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000730v1_decoy LN:1216 M5:2a49da07788e874c277958962ecb0df3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000731v1_decoy LN:1215 M5:c382bd37421a81163d88288a6f7cde1d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000732v1_decoy LN:1214 M5:2987b50923434c160ee2c370f1a0665f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000733v1_decoy LN:1214 M5:2cef32c11c190dec442c6760ce3b95d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000734v1_decoy LN:1214 M5:3320cf99748892c60e02c948326286eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000735v1_decoy LN:1213 M5:ed1d21ad71609ae43a9b6bcebb49639f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000736v1_decoy LN:1212 M5:b586f3157d6a08d059ff88c2a8b09110 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000737v1_decoy LN:1209 M5:7ba7bce4d6531a5524c73a86b5bedbb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000738v1_decoy LN:1208 M5:04bd45b790033b8311ed85dea3015e01 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000739v1_decoy LN:1207 M5:347ce8f1f6a746892189ab8494e8aaa8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000740v1_decoy LN:1207 M5:707de4a5497ea34c4e4f0bc0c85de3fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000741v1_decoy LN:1207 M5:be1fcf2149a75c654ccdcd7bdf0f6fbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000742v1_decoy LN:1206 M5:4e1effd0109053a328e58366f9f97082 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000743v1_decoy LN:1206 M5:9f9dfe453260eb10828d18745f7f5dcc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000744v1_decoy LN:1205 M5:855e900b59e0654686ce1a3d9955d650 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000745v1_decoy LN:1205 M5:137e80b53681aa0957f4489dc3bebca5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000746v1_decoy LN:1204 M5:d5ccfc0afa0a8ffeb39566a3edbd5831 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000747v1_decoy LN:1204 M5:2d8e3051fce1f6364064a61a1015f902 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000748v1_decoy LN:1204 M5:4ae1aece0937c17d93e0b54aab43444f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000749v1_decoy LN:1203 M5:108a33f55fef0274c3265bbadc509de9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000750v1_decoy LN:1201 M5:6ca12ea72ce31843c3a72b1519b0f252 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000751v1_decoy LN:1201 M5:85e6c0c98d00cefe52487084168379f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000752v1_decoy LN:1200 M5:6db13990bde5be0862c8524915fea770 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000753v1_decoy LN:1200 M5:e2708863bd36e41c2e8ea5a6b3ec5110 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000754v1_decoy LN:1199 M5:beae786e8598f79032fc8c16208aeb00 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000755v1_decoy LN:1198 M5:0a56caad49417b3587e394dcf70fb6f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000756v1_decoy LN:1197 M5:7f43dc6c55686b78a0ca14bb83eaf8c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000757v1_decoy LN:1196 M5:ed9e0446d6bb51755db408158805d507 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000758v1_decoy LN:1195 M5:cbb14c85e5696a49ce697e346634e422 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000759v1_decoy LN:1194 M5:af5aad17aff7705f0fd96e58a32a59d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000760v1_decoy LN:1194 M5:b1a7eab6e2cc0e62933274443fed0a24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000761v1_decoy LN:1191 M5:23fae8306c0b4a5bf7d0941294736058 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000762v1_decoy LN:1189 M5:5b7ba9e9888da831b0aa4565b412bcc6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000763v1_decoy LN:1186 M5:b39edd9fdfea3b1d9644fa805f33a2b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000764v1_decoy LN:1186 M5:ad284b4b4daf92a4456309e77375468a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000765v1_decoy LN:1184 M5:98516b9021a46bcc79d7386ac6dc34c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000766v1_decoy LN:1183 M5:64d3e55e37f2e86e15c96ef1d879bba1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000767v1_decoy LN:1183 M5:bb4656c8fbc2436252c45a1da9ec9583 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000768v1_decoy LN:1182 M5:fef2856f46d2c7724e666eafdd402ebc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000769v1_decoy LN:1181 M5:987931dd2bf6bac95e341d207f146623 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000770v1_decoy LN:1181 M5:eac4c9bfba221a54e6f9e50cbda276fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000771v1_decoy LN:1181 M5:cf5eee43e4ec7820ecca7ad7864f52ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000772v1_decoy LN:1181 M5:0b58fd78215049a25bf9904c8d82de05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000773v1_decoy LN:1179 M5:bbff47313bf9094c388338d41292f0da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000774v1_decoy LN:1178 M5:0e47d0853364f93c33da6738f79bd494 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000775v1_decoy LN:1178 M5:f12f849bba7e72b4f1f60d7aad6a21a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000776v1_decoy LN:1177 M5:1b10327e63ec134bcc76d1d55f9e78d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000777v1_decoy LN:1177 M5:c87caf65afb409c1775c034ed6b2c791 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000778v1_decoy LN:1171 M5:d25c5de51ec9619b03cce2f95298e8a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000779v1_decoy LN:1171 M5:3e4f5e7a0753a3239b3bc0d49eeaf461 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000780v1_decoy LN:1171 M5:b988438edbb2bbad2f8c1dd32c116f59 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000781v1_decoy LN:1170 M5:41b5e67b7143e5bf81dbb61b81a230a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000782v1_decoy LN:1170 M5:ac3de3666d0730b5fb7f44a84f9c4d1d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000783v1_decoy LN:1167 M5:7187d1e3ed138db9f1610a9c4dbefeb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000784v1_decoy LN:1167 M5:a1f7192900c80cfabbb39df8dc8d3c48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000785v1_decoy LN:1167 M5:80ff0c82d9a5bc156c3c0bf340bd68ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000786v1_decoy LN:1165 M5:688f5bf8eb90c874eda7108fcf283889 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000787v1_decoy LN:1165 M5:f768117d9e7c2526c123ea228e2cafe9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000788v1_decoy LN:1162 M5:039765c983a107557b8c3ec0a025e44c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000789v1_decoy LN:1157 M5:220dbbeab5a5d61415cdfde9f50b5766 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000790v1_decoy LN:1156 M5:23d66ea0a7a7d160c9413d93f5ad0162 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000791v1_decoy LN:1156 M5:5cb979a509d0b6052c6e0a55be36cc00 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000792v1_decoy LN:1154 M5:b0ee554cec6a7fca1a6556932643d8a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000793v1_decoy LN:1154 M5:0370a3a0105a0683cbd802403418f170 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000794v1_decoy LN:1151 M5:8e0e1ffe5b02de8878bd4d564bb6323c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000795v1_decoy LN:1151 M5:7bd0473f38ad03caa52a001a9fbb7354 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000796v1_decoy LN:1150 M5:5a37e3660ee2c75c170b2b5b40e9fb6b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000797v1_decoy LN:1150 M5:490b01f6455790bd5df9ffc337520f37 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000798v1_decoy LN:1147 M5:20582882471f3bed3cd0120b82323ac3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000799v1_decoy LN:1147 M5:0e5917e383190727d973a070137a2fbc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000800v1_decoy LN:1146 M5:4bd3ad205dd76c8fb5e52879cad2ae96 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000801v1_decoy LN:1144 M5:b97e8d5a835434eb2a718b9e61b8108e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000802v1_decoy LN:1144 M5:2df1004e75f2e124d3578d82432b9495 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000803v1_decoy LN:1143 M5:f38f2b568b969edfffe4cb768ffbf1f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000804v1_decoy LN:1142 M5:9aab8eca5e4d310eded1b7375ddc7cf6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000805v1_decoy LN:1141 M5:9d60d12e15605e5bdc4e95d5507c5fbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000806v1_decoy LN:1141 M5:ed137b8aa6491c9545e9486d7412e617 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000807v1_decoy LN:1140 M5:153c981d613562052e1fb0b115c4534f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000808v1_decoy LN:1138 M5:0338a9928d761051eb989aba5a478df1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000809v1_decoy LN:1134 M5:58756cfdfe491443b22624377976b379 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000810v1_decoy LN:1134 M5:684bc9fdb4e26e632a8183d8802beedf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000811v1_decoy LN:1132 M5:cbe47496e2df2406ca4e66332ba1ec10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000812v1_decoy LN:1131 M5:4408dcacff9d3e25b64da0a9935a7bc3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000813v1_decoy LN:1131 M5:58cc2832c5530babe80d72360b82afcb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000814v1_decoy LN:1130 M5:c537aaab4d2fffc792fff332ba26df17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000815v1_decoy LN:1127 M5:c10ad449537826ed9790a8e7a91cc9f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000816v1_decoy LN:1126 M5:43067a55481ed1a3a07a02ad65eaa2df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000817v1_decoy LN:1124 M5:68a35e6dd8db1522069b2a5cee954207 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000818v1_decoy LN:1122 M5:488cfcbe81f47dcfda704c5c28ebbe03 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000819v1_decoy LN:1122 M5:de91661f937117bf675c5f21a54ab668 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000820v1_decoy LN:1121 M5:5e6a5620500506981d3ab73624f63d9e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000821v1_decoy LN:1119 M5:fb748af1360cb775a390f0e931dd02b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000822v1_decoy LN:1119 M5:6b4f3cf5f29db2ef1bddb02c5828ce30 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000823v1_decoy LN:1119 M5:90f491e70c191edb78a37852f165ac39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000824v1_decoy LN:1119 M5:53358ae1c3833697bd5f0eb0f8ac79d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000825v1_decoy LN:1118 M5:e36d0f228ae617024efb91c918776dbc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000826v1_decoy LN:1116 M5:28d254c1b706d0bf3b10df07746930cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000827v1_decoy LN:1116 M5:08d17cf8bbbea61863d1111ebec35b21 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000828v1_decoy LN:1115 M5:6ab6da5d7d22274a6ed522846b55ebc0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000829v1_decoy LN:1115 M5:798a4b9e0b964f40d8c23e5cc3336db7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000830v1_decoy LN:1115 M5:9cbd8613556330a85d27a64185ea84cc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000831v1_decoy LN:1114 M5:83cc1a5967022f8e841c81fe75d58ffd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000832v1_decoy LN:1113 M5:01c1108b447f8fc1ac66a83a185cf320 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000833v1_decoy LN:1113 M5:7f89f5d60ad86ff56b75b22948d678c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000834v1_decoy LN:1110 M5:5a70b2a58e433469ccb66822189128d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000835v1_decoy LN:1110 M5:1e38e29d6d62fd249bf64655d6f81862 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000836v1_decoy LN:1109 M5:96a1d9adc17f059b56e67cfb6937a345 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000837v1_decoy LN:1108 M5:45ea81c798eb106c5436246caa66b2ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000838v1_decoy LN:1107 M5:1814f68b7f841bfe03b315aa32382c00 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000839v1_decoy LN:1107 M5:48441754ca4953c7e3a4a0c8d47ee1e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000840v1_decoy LN:1107 M5:7f28434a41e01b99a0d611c226b8dae7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000841v1_decoy LN:1107 M5:d18ae976cb8e092b2164fb5cf8e96c57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000842v1_decoy LN:1106 M5:7d6bf428559a5a4c3e493b755ebe5524 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000843v1_decoy LN:1103 M5:c32717ad22315f9c16c5ec2cc21c930d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000844v1_decoy LN:1103 M5:a1754553d128a5a63a67e63de2fd3738 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000845v1_decoy LN:1103 M5:39f91ddc96023a6c28b74bce8ab2612c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000846v1_decoy LN:1100 M5:82964c295c4313b285a6149d3c1566a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000847v1_decoy LN:1099 M5:b6ad0bd8376da6c84aad8cf5fe7bc6d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000848v1_decoy LN:1098 M5:e14ec564d8038b588e7bbcc8b2ec0a9a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000849v1_decoy LN:1097 M5:7307c9a55c6540dd0e2d41858ae31cbd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000850v1_decoy LN:1096 M5:a2998a406a8123e41e824e2ede54ed2b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000851v1_decoy LN:1096 M5:e62b4e85fecea83baffc447a08ca6785 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000852v1_decoy LN:1094 M5:6e5c8b4b68744e944eab55ecb8fb5844 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000853v1_decoy LN:1093 M5:24331159404d19f94c9138da50577e51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000854v1_decoy LN:1090 M5:2929cff1249a75eefb4cfffd96d62464 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000855v1_decoy LN:1088 M5:fbb037cbafc5f686b9ab73e07f0ffcfe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000856v1_decoy LN:1087 M5:211034f9737003efb857df7e977d5277 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000857v1_decoy LN:1086 M5:368e68935f97965228ac78bb2aae203c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000858v1_decoy LN:1085 M5:ce2ac36f0b0097282b1bff60c135f74e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000859v1_decoy LN:1084 M5:3418f70e7ae002b97228bff0e077080b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000860v1_decoy LN:1084 M5:b8e4715e539923de16e145b5eac6191e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000861v1_decoy LN:1084 M5:fc5500f691a93bcad35796e610743159 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000862v1_decoy LN:1084 M5:e59c0bee43efc00f925e98deafcb1339 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000863v1_decoy LN:1083 M5:3f45413c28190577d1c4837a73d4a27a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000864v1_decoy LN:1083 M5:5e5b283ed71b787f5a90e2a85cae8fcc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000865v1_decoy LN:1082 M5:50f12f0b974f354351bb88c197b21126 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000866v1_decoy LN:1082 M5:d678f152fcc7e46daafe921a0a41337d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000867v1_decoy LN:1081 M5:b861e354b7da07ac9ff8f421b6caf341 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000868v1_decoy LN:1081 M5:0eb25de463e630b62cb8c32bb42f6ad5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000869v1_decoy LN:1079 M5:47743f7eabd8f065bf223bf5327dc730 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000870v1_decoy LN:1076 M5:43ee859a8e75e455f0f1bdbfb155b6c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000871v1_decoy LN:1074 M5:8de0f0af578c623d46ac53461a51a3cc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000872v1_decoy LN:1073 M5:788f48af377f20246f7f9a21e90b0e0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000873v1_decoy LN:1073 M5:45c2f5e2b5413134f6030f49be58ba4f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000874v1_decoy LN:1071 M5:86a4e6ad2956475caac9b348e6e7f8b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000875v1_decoy LN:1069 M5:22ee1b95d0dcca48ee2f77ec8135e0d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000876v1_decoy LN:1067 M5:3f76ce94efafe5dd1634e6d51aa6d1d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000877v1_decoy LN:1067 M5:5bc259606f224eb610c64d5e5d55321b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000878v1_decoy LN:1067 M5:9449555579bbe1f92046e6c9b1d6eb4a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000879v1_decoy LN:1066 M5:2777151312e10fd89af514515090fcca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000880v1_decoy LN:1065 M5:ae8283b9a8386e65951be3f19e3b7006 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000881v1_decoy LN:1065 M5:79e7ca97d9a943a065f8d902b9b01244 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000882v1_decoy LN:1065 M5:0991862c1ee6015ab6ba1008ec3ff69d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000883v1_decoy LN:1065 M5:d68bf7942659eebe8f4973154fd1da22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000884v1_decoy LN:1065 M5:dd08ea8e4c5ee4d1f942b2dce2296cbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000885v1_decoy LN:1064 M5:a5179394ece1fe9e2392cac1241bcacf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000886v1_decoy LN:1064 M5:5ad23055bdc140edb211af99bf7d76e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000887v1_decoy LN:1064 M5:5fb9770dcbf52d1a7f41c9d235a9ceeb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000888v1_decoy LN:1063 M5:68844d1f612bcae36302b17758f78e86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000889v1_decoy LN:1062 M5:844d181d05473e9b6d06b5defabf50d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000890v1_decoy LN:1062 M5:224848a8b0947ecc656065423b7862ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000891v1_decoy LN:1062 M5:de33c1fe76fea17846a96ea7bc9e91f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000892v1_decoy LN:1061 M5:919160e2c6fec7a7c92d823d76b66027 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000893v1_decoy LN:1060 M5:f79c0acfc167625b085df24f5058e8f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000894v1_decoy LN:1057 M5:6cae16f687b6453bea4343f3a72ea5d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000895v1_decoy LN:1057 M5:7b95591d319231c96c2db2ab196e568c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000896v1_decoy LN:1056 M5:59477a60f18aa435be9cd8e8cb8958fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000897v1_decoy LN:1055 M5:ef9e2ac86b6dc21510af5a157ffdd9a7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000898v1_decoy LN:1055 M5:8a426cfbaa95d3a4ff9de0b8cc401ed0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000899v1_decoy LN:1055 M5:071ea5ccf103c1e5bc64476b69297cae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000900v1_decoy LN:1055 M5:a0183c0c525e9830c2487ba397d48984 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000901v1_decoy LN:1054 M5:fbc0b340afbabfc63b03b8ec4caf77ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000902v1_decoy LN:1051 M5:5f5a077b53495abe7fc001c49c8b9306 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000903v1_decoy LN:1050 M5:69420a1791c126a89681a6620ca82d48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000904v1_decoy LN:1050 M5:9c4f6e5286d911f86ede7780174ee24a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000905v1_decoy LN:1049 M5:0940c85e47df41bd6052ec8f8ca76da3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000906v1_decoy LN:1048 M5:d1bb56cbbe04f366cb8b9e00e16c0292 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000907v1_decoy LN:1047 M5:b1bfe8c8a961d7d57914f4d7c4244100 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000908v1_decoy LN:1046 M5:e48424ce325e8f78e32af41d56e93243 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000909v1_decoy LN:1046 M5:2b08cbae6f429b208c980cf721fc1a43 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000910v1_decoy LN:1046 M5:43dfe59c93ea059ca8557bb58d0d3e39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000911v1_decoy LN:1045 M5:4a62b434c567f91554aba4fe9d29bee5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000912v1_decoy LN:1045 M5:9dda9f1dca0f324d3ec28a2f95399b6f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000913v1_decoy LN:1045 M5:f7d06c828a959e093ca5c4fefcaed79d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000914v1_decoy LN:1044 M5:9565e8a0c6d2b4911f44a2dec3319fec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000915v1_decoy LN:1042 M5:63910bd693964213823ca9ff1e270883 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000916v1_decoy LN:1041 M5:9350358a6726a178c8e3c5c038a58b5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000917v1_decoy LN:1039 M5:1c73f73eb55cd6290be7beede9ad10f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000918v1_decoy LN:1039 M5:95035075514c5d5ad7852ba2c1b2b649 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000919v1_decoy LN:1038 M5:f8b064906cddf6b316b6aee040120925 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000920v1_decoy LN:1036 M5:510d0277b9afc44d0f3a07b3327a111d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000921v1_decoy LN:1036 M5:d7a9e2e36d916e57d735f657035a9829 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000922v1_decoy LN:1035 M5:318ac9befd9a2449cae3c371dec8e628 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000923v1_decoy LN:1035 M5:6af821ff48f6593ccd322ce697bdc253 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000924v1_decoy LN:1033 M5:bdd1f96a69f748c2a2fc4b21e31c2f06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000925v1_decoy LN:1032 M5:5b23ae1b5786a5a5eadc92b69bd836c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000926v1_decoy LN:1031 M5:b06aa5a4fb487a467429373242a18923 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000927v1_decoy LN:1031 M5:6fd21c6cec0c8086aa77d26c6d200aeb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000928v1_decoy LN:1031 M5:196c591c8593f2c090224ae6cec80358 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000929v1_decoy LN:1027 M5:b7eb33328daf9d0ae316619a61384cec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000930v1_decoy LN:1027 M5:a1112db1101bde90d172ceb0b455adc0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000931v1_decoy LN:1026 M5:42be32c5547cde39b2ccc77ac8e41c3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000932v1_decoy LN:1026 M5:62f5a154eeb5e3613de4f255efe75565 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000933v1_decoy LN:1024 M5:69e6c66e0db5f669e7762df739c9bba6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000934v1_decoy LN:1024 M5:f2b5de034be298d37051f37061d1748a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000935v1_decoy LN:1022 M5:d81ab37920ea303d70fec80162569190 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000936v1_decoy LN:1022 M5:08f1fd0b0e3cb4204a68c0bd7c5c57a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000937v1_decoy LN:1021 M5:4c13817a5ba74eee0c03aada914a0243 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000938v1_decoy LN:1020 M5:e4ef90447f68425ac775770584c2ac6d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000939v1_decoy LN:1019 M5:d7d108b06883f65ce1ec9f6a55b4842e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000940v1_decoy LN:1018 M5:3e1e177644a21aa70ff44fba9753313c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000941v1_decoy LN:1018 M5:6bff6cc8151a99c46a864ea703c2f8b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000942v1_decoy LN:1018 M5:e7f0466b453823ed7d075d4a2ede4a7b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000943v1_decoy LN:1016 M5:3d62e68db3dcf0e169c55174dcbd79c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000944v1_decoy LN:1010 M5:7b808bb60e41db8c1149f10d7bd5465e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000945v1_decoy LN:1010 M5:8f606e0943101b0656668a2ae35e055f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000946v1_decoy LN:1009 M5:5af16ec72873d3cf402dd411e1bbaafa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000947v1_decoy LN:1008 M5:ccfac7e6bd5f5a5fb98e773922be5b3f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000948v1_decoy LN:1007 M5:d5e4ec92647dc97d7ca5c479effc5698 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000949v1_decoy LN:1006 M5:53c640d8a27c49f44643fa6cff64c7bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000950v1_decoy LN:1005 M5:4f20464a0e34b4c139f58567dac67a9d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000951v1_decoy LN:1005 M5:47b4982ddfd84f1b626e9ea7b0859169 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000952v1_decoy LN:1004 M5:77403eb0765e6a6422307122ee3a0544 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000953v1_decoy LN:1004 M5:74caf00415bd1b5764ea6082c0945b27 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000954v1_decoy LN:1003 M5:811dd1c7e02a779772cb0adcd330822d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000955v1_decoy LN:1003 M5:b217fa3505a5024cd26ddcf42d514a4d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000956v1_decoy LN:1003 M5:c90b2e71160e8f5cd6d23a740a17841b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000957v1_decoy LN:1003 M5:3bf126803f3335d9227e753cb43060dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000958v1_decoy LN:1002 M5:3904df1efaf5438d8e060141cf4dd6a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000959v1_decoy LN:1002 M5:ab5ef802456142b25705723663909e35 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000960v1_decoy LN:1000 M5:ff514a3d2ae42b584daf88b9a9a8751d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000961v1_decoy LN:1000 M5:eb02c1a37ede0d1a737a6c8c984eb06e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000962v1_decoy LN:8358 M5:567a758298b590bb1f4a159d81b84283 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000963v1_decoy LN:7932 M5:fd0a26e270577d3a6cc4cb9256e21cc2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000964v1_decoy LN:6846 M5:4fd9f0f9e88fcd125702c11dfc2c4efc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000965v1_decoy LN:4591 M5:31483ec110747cfcac4dc1e36e8758ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000966v1_decoy LN:4041 M5:f4f7ba029902c50fa77ed2b9966eb1a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000967v1_decoy LN:3841 M5:00a9379bf89f4556f66988ab0193b4c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000968v1_decoy LN:3754 M5:bb2217ed56094a266a3fb1b82e6c6a23 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000969v1_decoy LN:3743 M5:5332a570edd08ee710cafaec398e0156 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000970v1_decoy LN:3702 M5:2b25e772f95c73582eacc01e9a048d9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000971v1_decoy LN:3625 M5:44cc5ae081c41e1c77f2043b88c70e0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000972v1_decoy LN:3529 M5:9c1927ed8f09b5ddff51f2f25450a4a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000973v1_decoy LN:3508 M5:04e36a548c184b404c00b7841ba45394 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000974v1_decoy LN:3359 M5:43e1fbfc042ef8b79879bd53c3b27521 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000975v1_decoy LN:3320 M5:4c9cce705cc18e643a96d433b0f0d80d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000976v1_decoy LN:3231 M5:090a5929ce6079dacc5408a9a7212f91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000977v1_decoy LN:3220 M5:b353222bd8f3e04b305911608d5b8b16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000978v1_decoy LN:3212 M5:d21eaa3001500aa078c0457aa0ecb160 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000979v1_decoy LN:3192 M5:87ce752d8b9611f39e6371d84b00dd68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000980v1_decoy LN:3092 M5:51fba58b7e482359134618d7115ef0b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000981v1_decoy LN:3087 M5:86e1c20fe0c0fc2ee5a4627c9dc91dde UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000982v1_decoy LN:3048 M5:0f4f9a676207d67aa7b835ec767daa3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000983v1_decoy LN:3005 M5:8cf60855c69aaa947ecffc2d85f7b836 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000984v1_decoy LN:3004 M5:1fe6d0e0f45dd1c103c83d965f4d065f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000985v1_decoy LN:2959 M5:5734404ace148ab496a0589c177d68d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000986v1_decoy LN:2934 M5:6091b4163a2558a3b376861ba46e49b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000987v1_decoy LN:2933 M5:867d88d0d6a7b4576b5f40df8aee32a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000988v1_decoy LN:2827 M5:fc01429a43e70e9f45d2e9b183f1cbb5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000989v1_decoy LN:2794 M5:8c97ce716667af6e7bf7b16598a2d1a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000990v1_decoy LN:2749 M5:460d8510eb2de1d5521b6303183eca65 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000991v1_decoy LN:2745 M5:bcb193217c039f3d327b106f7bbb1eb2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000992v1_decoy LN:2733 M5:38d3109378fc051e0d6d30c9c768c361 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000993v1_decoy LN:2698 M5:7909dcbf170e075fd5ae77f2de6658bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000994v1_decoy LN:2665 M5:90d637dd87631542f98b8a2fb31e45d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000995v1_decoy LN:2634 M5:a5125437bf818a2f4ece1a2cb7ad4e5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000996v1_decoy LN:2492 M5:82de7c0346b508963d47eaef107ba2ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000997v1_decoy LN:2489 M5:904eab4180cdf8accc4e926f65892e16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000998v1_decoy LN:2468 M5:5a16ed756cd3e8451225c0c89c299ac3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01000999v1_decoy LN:2414 M5:a7f82c5206197f2b60bf6e21e8406ff1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001000v1_decoy LN:2395 M5:fa87a3decbc163bd5c4be47f26ce4c72 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001001v1_decoy LN:2356 M5:c1764faa777d0cb7e6d10ff41a7f4d46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001002v1_decoy LN:2339 M5:a71335121d2395b90f88701d0d395b28 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001003v1_decoy LN:2310 M5:e7727f2a6ea39391478a70b270b9a4c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001004v1_decoy LN:2288 M5:27c3653c8e9470329db519a7e855887b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001005v1_decoy LN:2285 M5:07a062d63e1175496ade57cb4924ac86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001006v1_decoy LN:2269 M5:da22a2b66c30d77f9c75ac5543b82df4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001007v1_decoy LN:2253 M5:521007d0a98d89311d4fef1f850e5c3d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001008v1_decoy LN:2203 M5:efd830cbd9f13d6f2e59621b80df1cc0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001009v1_decoy LN:2176 M5:35223579474d332cd9c25530d391cb37 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001010v1_decoy LN:2159 M5:c6451a9867c60bb9414b9f531733227b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001011v1_decoy LN:2155 M5:c9a15197997fcc229a4ba16daaef7ab9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001012v1_decoy LN:2149 M5:8fc79f00adb9b9876fc0c760212279a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001013v1_decoy LN:2129 M5:e0053f5c6a91ee7561e587740719a89a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001014v1_decoy LN:2116 M5:c4594c9954ddda6bf892d1221deb1f5a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001015v1_decoy LN:2113 M5:a604e6df460c882c18dd0b761e7da09a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001016v1_decoy LN:2098 M5:1e7f643633169955a530300b89b41499 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001017v1_decoy LN:2066 M5:95e88f3c4f847fe97ff528408aee9885 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001018v1_decoy LN:2066 M5:50eb710c12d63d96710eead27936dbd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001019v1_decoy LN:2059 M5:80e50b65d76c95f06a65b213d6e22e3d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001020v1_decoy LN:2047 M5:1e5494b45601a7dacb84814da24724db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001021v1_decoy LN:2040 M5:0fd285ea19d36ac7fbfeb49f98d0e3fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001022v1_decoy LN:2030 M5:4d3b39f0ab2f0f051d917cc94faeae9d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001023v1_decoy LN:2024 M5:a4cef27d7ccd380794cbff4dd6eb41e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001024v1_decoy LN:2001 M5:e6821e55e6dbf96dd5ab53b59be17ce5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001025v1_decoy LN:1992 M5:dda157c4b492e3c1a4e277e1dab4aa7e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001026v1_decoy LN:1981 M5:4166a173f0411e5579f9d3ed8aacd959 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001027v1_decoy LN:1979 M5:88e1e86ee00ad030c8e1f25e49ad569a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001028v1_decoy LN:1957 M5:26727871d8918ce12f1e7fa8f5914c7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001029v1_decoy LN:1953 M5:9b38c1ef5a1efb5065c9f3dc102ff199 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001030v1_decoy LN:1944 M5:85264a3258514a27228a82bcec9ea08d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001031v1_decoy LN:1936 M5:a7347a7344187fa04690bfdffc33512e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001032v1_decoy LN:1932 M5:4030d4f8fc378be7ab110a327a3a7f7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001033v1_decoy LN:1882 M5:ca4279d8b8d3f3b766ec81b34c402aa0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001034v1_decoy LN:1878 M5:e5d3efe3296f7f5ffb2193bd5be2aead UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001035v1_decoy LN:1870 M5:1c898a94d86446ff18aeae6c9836d2cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001036v1_decoy LN:1821 M5:8f2121509d8a9d3e95e78ceeaca8db95 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001037v1_decoy LN:1813 M5:1111c9f5ecffa495ea2a9408094b8fb6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001038v1_decoy LN:1809 M5:0ef16d8e23134785f99146b8b3cada3f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001039v1_decoy LN:1804 M5:11e7f4e8d60fef86d7fbefca0fabf5df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001040v1_decoy LN:1797 M5:42437cf6fa6210fd9b127e7487d14e4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001041v1_decoy LN:1791 M5:1b98bd8020f173b5205948481dd39658 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001042v1_decoy LN:1781 M5:7460d29d17ba264f4358d5876a383fac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001043v1_decoy LN:1766 M5:1599e276d124e4a9aa2f66a6300f8a3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001044v1_decoy LN:1764 M5:fba6f4c5474d22767649a8328d8f3670 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001045v1_decoy LN:1743 M5:8b83d468225e1a45cb1846dc726f2562 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001046v1_decoy LN:1741 M5:8adc2f7bd9e61fde6e9824026c0a2430 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001047v1_decoy LN:1709 M5:7363ff9bc2e506ad5041f70fc083e7fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001048v1_decoy LN:1706 M5:ef2492257bbf08783a4db0aecad49550 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001049v1_decoy LN:1701 M5:9c322f81502c32f0c8f405ee752a4f44 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001050v1_decoy LN:1689 M5:5fe371c4096ae41150f76981d68dacd2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001051v1_decoy LN:1646 M5:ed85a7af0713ab3b3bcdf61bafeedea5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001052v1_decoy LN:1641 M5:97b84c347da74fc7614b6363ba4f28e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001053v1_decoy LN:1639 M5:dc79cb73595caf151cffe0be4286ce1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001054v1_decoy LN:1636 M5:221da4dd1119306af9f8fa1bc567f6fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001055v1_decoy LN:1632 M5:6f572e5698e138bf1e7b1edba75588f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001056v1_decoy LN:1629 M5:fd6f43c6fc2542ce63298bdd465a38a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001057v1_decoy LN:1623 M5:4a44475e85813d0b54e2afdd3aa8315b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001058v1_decoy LN:1622 M5:d30e3342b06bd2428d574ef50226a9b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001059v1_decoy LN:1622 M5:0e6aaefcfab75455e8f59e751c8d44a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001060v1_decoy LN:1619 M5:e91e56fee1ae99144354eba8e5ec571c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001061v1_decoy LN:1606 M5:e266b715eded02faa272225186a213dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001062v1_decoy LN:1593 M5:74d3a9f8a38387de10a759100154372c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001063v1_decoy LN:1592 M5:efcf64c62b2233135f5794d7b9e2f4cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001064v1_decoy LN:1558 M5:bbc8fdfa71746e3e396e745a1fc9d63d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001065v1_decoy LN:1545 M5:fc836522e323b4779fcc5b16f2d28505 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001066v1_decoy LN:1542 M5:6fe808dc84087dc7d8418eced9744173 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001067v1_decoy LN:1540 M5:9c582e02bbde278105b2764116a44ef6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001068v1_decoy LN:1529 M5:9f25be63cbf7cc5da1d1bc6fe23db799 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001069v1_decoy LN:1518 M5:772df05b40eabe6b084af8cac402aef4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001070v1_decoy LN:1515 M5:bda033712d93ab6c918ec5b0fa58f343 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001071v1_decoy LN:1513 M5:faf46f2a8ea7b8d1d0282aaf9e10141b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001072v1_decoy LN:1507 M5:12e6bce1a4e70c91a764227eafe6d229 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001073v1_decoy LN:1504 M5:c757ce71d69487d184714bb6bf879036 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001074v1_decoy LN:1499 M5:d6645febc4c0e7fef261124c74ecff8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001075v1_decoy LN:1495 M5:adf3668dcf991b59a38c281c3d7b5647 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001076v1_decoy LN:1495 M5:2edd8f2a644a90e4ca21826bb31402dd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001077v1_decoy LN:1492 M5:3dc6cc3ffd2a9b7966ca3e8ea12dc81f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001078v1_decoy LN:1492 M5:308ce742886a73a84ab8cb90370451ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001079v1_decoy LN:1489 M5:39d5f26be15da228d1d38b408e0a392c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001080v1_decoy LN:1485 M5:2a52bf85b6a45c79c92a6c6027abf122 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001081v1_decoy LN:1483 M5:d22840c21eb279736a5a8262ec8a66b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001082v1_decoy LN:1473 M5:d6b57113fdeadd16b92fed725a02d12d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001083v1_decoy LN:1470 M5:5b5ff1a6c8fff55ac6ebd1d9bda4fd80 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001084v1_decoy LN:1463 M5:80030f88512ee394cc4e9a81b032e17a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001085v1_decoy LN:1460 M5:2587132a077227d0b8742ec9439f95b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001086v1_decoy LN:1458 M5:5366ad05ba9fe3bdfbc51dee8759c348 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001087v1_decoy LN:1456 M5:1d39f2ea075eca983ca23f12efc47f22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001088v1_decoy LN:1453 M5:020a82dc938b6e53e904f477268c7d5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001089v1_decoy LN:1443 M5:541cab251db4f8356953dfc20e8cb327 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001090v1_decoy LN:1441 M5:a563ca647fc20b65ba7baf52e3eb4648 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001091v1_decoy LN:1426 M5:d096cb367c98d6804b4a1b205054c9ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001092v1_decoy LN:1425 M5:4c1a5daffb2303c754e4383ec3bff31f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001093v1_decoy LN:1418 M5:5305c46f4221a62f8001b24ced994801 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001094v1_decoy LN:1413 M5:8d8667fd137515e7c5d31b0b942a7525 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001095v1_decoy LN:1413 M5:e38f0eb475fa6f2aee1b51ea785af3f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001096v1_decoy LN:1412 M5:88ee82e753efc8d4fbd5f1e02ddff9e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001097v1_decoy LN:1407 M5:732666474ada2d4fe1316edd249b2f5d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001098v1_decoy LN:1406 M5:f63dbabd25745821024183fe0082257b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001099v1_decoy LN:1396 M5:0102bc3c8078cc16bddfa125f005370a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001100v1_decoy LN:1390 M5:181bb8fe5095e667506c66e213f3b51d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001101v1_decoy LN:1382 M5:545aae1204533fcf75e6c622471d3061 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001102v1_decoy LN:1376 M5:a69102cf73d82bc4298048ee7eb0aa85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001103v1_decoy LN:1375 M5:61f67b4a0c7573bf583ae5a051e7aaf3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001104v1_decoy LN:1371 M5:4b33e5f1a51a79cdc62fd070cf874f91 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001105v1_decoy LN:1367 M5:4286f9ebcff872815a9246b3a2596781 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001106v1_decoy LN:1364 M5:9a3f9b88cf1926d9c75b673647ff46c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001107v1_decoy LN:1356 M5:b54b34217f1c3efb1ba9d4477a0f2045 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001108v1_decoy LN:1355 M5:c49fb7670582bf2f81413071056f61ff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001109v1_decoy LN:1352 M5:32abeff50bcab272795c72f7b7edd46e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001110v1_decoy LN:1350 M5:9660451ad55da2148a00b49a126176d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001111v1_decoy LN:1346 M5:c6722a471b0e8cb1384d59ff72cc732a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001112v1_decoy LN:1345 M5:6ffb665540f55661d5ccf40795cebd35 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001113v1_decoy LN:1340 M5:e0ba11f5404075a40b0072d19c2bced2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001114v1_decoy LN:1330 M5:ccb7f39ff649bf360b5b4186cf18c01f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001115v1_decoy LN:1329 M5:c52f3a9196ca3b03d2a02680f15419e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001116v1_decoy LN:1324 M5:f07d0c07c8951023331599b2df20380d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001117v1_decoy LN:1316 M5:8195f619c45e8d571a25264aa6e7a65c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001118v1_decoy LN:1307 M5:49f9986c45cac526b89e5520a9baa4dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001119v1_decoy LN:1304 M5:fdbf4160ba84788b20bd855b64cea30f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001120v1_decoy LN:1304 M5:edc34fe61ae9a3b15bc5a4ad8d291271 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001121v1_decoy LN:1303 M5:6aea0ad92108bcebca7f7886b75bf9b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001122v1_decoy LN:1301 M5:ccf0d28aa2b30e1d8e7ef2eafe01e849 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001123v1_decoy LN:1300 M5:8bce51ab0470cf3f210d3b25582c2297 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001124v1_decoy LN:1297 M5:01e9ba63de5b8b6e1a07efb0b1179f20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001125v1_decoy LN:1296 M5:dd2148d2b631c1a7e61a18155b56698f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001126v1_decoy LN:1290 M5:9e7639e4c222198ef6503cc2e1c7577d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001127v1_decoy LN:1284 M5:1ed6768a3d0449e96d3939d08102f424 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001128v1_decoy LN:1282 M5:a8d94de0900b31bf20f7c16fa5eb29c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001129v1_decoy LN:1281 M5:30eded62211ca354db518348aab06f6c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001130v1_decoy LN:1280 M5:35b60389b57134465f6d190d5955cf46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001131v1_decoy LN:1279 M5:448c65a5e874d8959bde564141823daa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001132v1_decoy LN:1272 M5:aeacf07664f294565febc34b1f8f640b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001133v1_decoy LN:1267 M5:df7730fbbc24afefb2749741f708f001 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001134v1_decoy LN:1267 M5:eb717a13171c7390c1c2e91a50473c32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001135v1_decoy LN:1266 M5:76896544c6b4b9c8bc2be23ce31fbe4f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001136v1_decoy LN:1264 M5:f3195b89752559a8a693e50e17a9d0c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001137v1_decoy LN:1264 M5:73ff474a35189bec700ddd7f25a140a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001138v1_decoy LN:1264 M5:b090d99b3c28f2a84a801018d5843220 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001139v1_decoy LN:1263 M5:a0baed3aa07a676a4fded91fd8edca4a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001140v1_decoy LN:1249 M5:0e81abee1c989a0f1ceebfa48b5eacee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001141v1_decoy LN:1240 M5:ddced69f19f82a08186fb39c0354eff3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001142v1_decoy LN:1239 M5:43ea2140da380099332cf0cb0490293d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001143v1_decoy LN:1235 M5:fe6e8b59583fb141e7b203df304e688e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001144v1_decoy LN:1235 M5:7051b4e9e0386119d27af1cbe5a2c633 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001145v1_decoy LN:1233 M5:703871732eddddd1c5ade422c3a7e105 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001146v1_decoy LN:1232 M5:a281258df9fd861e7ca6bdb869fd27d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001147v1_decoy LN:1230 M5:d0939ee2b936624fd9dc5f0bb2a0cff5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001148v1_decoy LN:1226 M5:21f414046e47b5e9c13d47b9b073c6cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001149v1_decoy LN:1223 M5:6e73b42728119fe2041f6c920248ed9a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001150v1_decoy LN:1214 M5:44865e67b99f28cb3bf0d5d89d259a99 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001151v1_decoy LN:1213 M5:062e3bbeb41213d0e68f944e1911f058 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001152v1_decoy LN:1211 M5:75ee5d3d8230d6db22a735709fd78e40 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001153v1_decoy LN:1209 M5:8ea8e29581777bdb7e7e2435b43a9123 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001154v1_decoy LN:1202 M5:5e4321979f4ad7f0e0630deaf3292360 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001155v1_decoy LN:1199 M5:de05fbc3c15237ca2c8d5c37f3f66dad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001156v1_decoy LN:1197 M5:81532092e3b56c94488f88b7b40525f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001157v1_decoy LN:1193 M5:ebc3be993ac1b9ab8acddc283066d160 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001158v1_decoy LN:1191 M5:9bb2ea3006a7f808a16cf62efac3d39c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001159v1_decoy LN:1187 M5:c5586dfb4606cba7d04c9bdb9b970ca3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001160v1_decoy LN:1186 M5:8ac59cbe50a76ddb4315fa75de02a202 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001161v1_decoy LN:1184 M5:1acac09b845dc4ff431c33916e819c87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001162v1_decoy LN:1184 M5:b3d7d809b498912a66c53542f6164aa9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001163v1_decoy LN:1182 M5:a06ad4e450a32bf31e43fa9794e6c635 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001164v1_decoy LN:1179 M5:2fa76b3fa2caedda0d3aa46b0071fd03 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001165v1_decoy LN:1173 M5:ba3aca12cb0c76e77a2ab28a3366cc21 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001166v1_decoy LN:1169 M5:7eaa353b323f8eadf6cafe90fce641bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001167v1_decoy LN:1167 M5:61d674b4fcb995535cf94aa7ee127946 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001168v1_decoy LN:1166 M5:38aa98ae17ee349012bee80c9828adfe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001169v1_decoy LN:1165 M5:bbd8f8056d59712fd77f6bbaddca57b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001170v1_decoy LN:1164 M5:807bdf141f9ae4103c56e43d949f531e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001171v1_decoy LN:1163 M5:cd8efaef0aa61647e740aa2b0e22ecd4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001172v1_decoy LN:1158 M5:3e53721e66305f3f2b4da98d998268d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001173v1_decoy LN:1158 M5:8ba71ae4bcbf61a48eabeee68296f62e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001174v1_decoy LN:1157 M5:b99ae11f6934e857cb9399ae6c41824f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001175v1_decoy LN:1157 M5:bbc9020c1846bb053b237d5b0478531d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001176v1_decoy LN:1157 M5:52700c360bd82baf97d7bc5fd70a9825 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001177v1_decoy LN:1155 M5:8e366717b85c08e01d0acc612724c6b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001178v1_decoy LN:1154 M5:53cfcde178b82cf94450e6ecc610ef24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001179v1_decoy LN:1149 M5:857f173eae9d86a6ed33b462c9607c5e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001180v1_decoy LN:1148 M5:ea231418525b44371f390a8a2a11af78 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001181v1_decoy LN:1148 M5:5a71efd43a930d44ebcb3574bb9cc031 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001182v1_decoy LN:1146 M5:c8f128dd7893d4ec3036e468d8bd6522 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001183v1_decoy LN:1144 M5:3cc0de0dd79e700a3369cf024230bc41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001184v1_decoy LN:1140 M5:fe4503df1d12b851a419140fa4b4173f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001185v1_decoy LN:1136 M5:224b91af2e2925de5d844cccabcf05ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001186v1_decoy LN:1134 M5:cfbd851609b789f2899a3be61d9127c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001187v1_decoy LN:1133 M5:463b232f53f888f5d3bf049f131ebba1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001188v1_decoy LN:1129 M5:1e9b1998943db973b5af6f8d3f8cc934 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001189v1_decoy LN:1127 M5:e780622b257020939988b4785d91218a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001190v1_decoy LN:1127 M5:7efc9fb11679f19ad35fe9371a9dbb1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001191v1_decoy LN:1118 M5:911cf50162dfce9f1bec2ae5f5ffcd74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001192v1_decoy LN:1110 M5:e8f80a36c02331c337e0b327ff8faf9f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001193v1_decoy LN:1104 M5:807ca6abca6229d97d8e363a0d01228f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001194v1_decoy LN:1104 M5:0f010a7025fef93df6efa5c5bf5a0f20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001195v1_decoy LN:1101 M5:1c2dc8ff99f4cc1ff11ad3d16b9f272a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001196v1_decoy LN:1098 M5:cb8ee97689963848f0ab08fab52ac3a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001197v1_decoy LN:1096 M5:0724eae831018e8ab18798c02eaa4f1a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001198v1_decoy LN:1094 M5:fefb3d4308c57ce507d9c50c88eaae9f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001199v1_decoy LN:1091 M5:a86fb31cc351320639079ba611824352 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001200v1_decoy LN:1089 M5:b75ffa24b8714c207a1d8de82e0812f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001201v1_decoy LN:1086 M5:0fcd8b558e0ab2ccf0b0d8bc3db23d07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001202v1_decoy LN:1085 M5:9e3b81bef0ba768284fd5cdc7b7ccf13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001203v1_decoy LN:1084 M5:35268d23224ae41608aa13bd7fdc7fdd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001204v1_decoy LN:1083 M5:a74077cc79c0fe324530f49b263b3307 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001205v1_decoy LN:1083 M5:cc88bb09f7a6ad37d5d5bf95400fc329 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001206v1_decoy LN:1079 M5:35f5a907b81ef8dbf77ec725790583dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001207v1_decoy LN:1076 M5:de787959b71e3c620c8ef7e41f6bf5b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001208v1_decoy LN:1069 M5:062313b0d5e67fee9d60100c7938397d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001209v1_decoy LN:1068 M5:93480c74c7540c0e7af8c2576e2b9dac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001210v1_decoy LN:1067 M5:8f8b744585f712f3e0f83707464cf886 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001211v1_decoy LN:1067 M5:929d9dd00e1ad8454c70511ea362a4e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001212v1_decoy LN:1067 M5:24aa3c508b845b2b1048334ce78332c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001213v1_decoy LN:1063 M5:29f3cd44f918aff7d3164e28bfddb98c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001214v1_decoy LN:1062 M5:12f432068dd357613e770c93cafe0452 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001215v1_decoy LN:1059 M5:47cb3f694ffe2d6ce654ed3b6cb5e3f7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001216v1_decoy LN:1058 M5:f49ebea87cb9479f2d50f484c3217850 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001217v1_decoy LN:1058 M5:4828d8055dbb4bfe4f703bb55a2ba1f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001218v1_decoy LN:1055 M5:6feebb18b6b36dd7c31078b901101e23 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001219v1_decoy LN:1054 M5:bbd43557ef56c3c1b056aa213470e130 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001220v1_decoy LN:1054 M5:135c9383230d02f4fa50cd9ef42c51d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001221v1_decoy LN:1053 M5:bf3402855155585fef154c6f26c2f42f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001222v1_decoy LN:1053 M5:55f5597ffafbca066600c886b78932ab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001223v1_decoy LN:1052 M5:777b4dde14fe0e0c5aff39ba12f25d7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001224v1_decoy LN:1051 M5:f46bdaf70049d1b5dcdcf4d9bb8bb616 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001225v1_decoy LN:1049 M5:a1ecf64987c3a271019ca2bcf6dfb797 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001226v1_decoy LN:1047 M5:c2548a36b128c6216d1689299d9bc6e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001227v1_decoy LN:1044 M5:57aaa99e04236628ee2e2c48688a28e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001228v1_decoy LN:1043 M5:8ab15ca1a985afa87ec7a9cf048505ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001229v1_decoy LN:1043 M5:ad851f750b29642813387f1b00a09791 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001230v1_decoy LN:1042 M5:794f6bbb5ddfaa81ad10c9f97e460d93 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001231v1_decoy LN:1042 M5:a88fd0ab44445757ce49b2886b3483dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001232v1_decoy LN:1041 M5:16cfe1e6b786b55e14e1c23b0010abd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001233v1_decoy LN:1040 M5:03f3627a2f7242721c8ba740808d67d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001234v1_decoy LN:1039 M5:2bd140282d454fa86b3d845400c68456 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001235v1_decoy LN:1038 M5:44928251b94a7ba7b6d78cfdc7b74903 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001236v1_decoy LN:1037 M5:0021932312a252327115aef207a22260 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001237v1_decoy LN:1037 M5:7ff32784d45f4a2b9d90d895152d8586 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001238v1_decoy LN:1035 M5:cafb9e327ae844419f531cc74ad34c2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001239v1_decoy LN:1027 M5:87b6d1b7895e0e98849ddb847f85e535 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001240v1_decoy LN:1021 M5:9f34ec8f23890b5c5ec129f3aa55fb28 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001241v1_decoy LN:1021 M5:52f88af4a5a6b8a7171df7143c99cec1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001242v1_decoy LN:1019 M5:093ab05e7ea2f698daf568af4a5f9314 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001243v1_decoy LN:1019 M5:322f96479243e771eb980526832e662f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001244v1_decoy LN:1016 M5:cf6212ddc0a137e657fec0764722dcad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001245v1_decoy LN:1014 M5:21eab5ad8f971f8753d0aee1004c1335 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001246v1_decoy LN:1013 M5:3c7ad60ac9394211fb081912b61e0f03 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001247v1_decoy LN:1009 M5:09f86dc178eeb4ecffafd9734a2afd5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001248v1_decoy LN:1008 M5:43929eb34efd2bbaf9f730d06a1e4799 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001249v1_decoy LN:1007 M5:494ad809a7d99f4147c2398821be92a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001250v1_decoy LN:1004 M5:589a72200353f7537edc3a9acccfe913 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001251v1_decoy LN:1004 M5:183d62e7208aff9604bbbf27702052f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001252v1_decoy LN:1003 M5:8f62a1d759dfd3a68b63b88c50e5fa85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001253v1_decoy LN:1001 M5:0bae6492e6a77abd67b50475fa94a80d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001254v1_decoy LN:1000 M5:6c3e8a079341eb05dd337a0413754070 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001255v1_decoy LN:1000 M5:39444bfd408b4e184afe6e0476041965 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001256v1_decoy LN:1000 M5:44d266b549830722a7a161606171afd9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001257v1_decoy LN:17929 M5:329d6c2a67aaff331fd020d0c9627310 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001258v1_decoy LN:9749 M5:446c5fb7d2e7af681df8773fae61fb27 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001259v1_decoy LN:8053 M5:83998e69640a5925a4a86054bce5f2ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001260v1_decoy LN:7826 M5:30c34924be761a9b459c27c08f1f85e5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001261v1_decoy LN:7768 M5:32d54ea636150f497c9484a714b37b8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001262v1_decoy LN:5691 M5:ed6cb5bb44a3cfa13f40e14e70bd5fda UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001263v1_decoy LN:5444 M5:40da5cd1840a6368532eaf7e9e4dbe8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001264v1_decoy LN:5077 M5:ea25764cf81ce3030520b86de80c9758 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001265v1_decoy LN:4990 M5:be003e2c8206a15f2159483f284c898a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001266v1_decoy LN:4545 M5:f2f37f70dde8161d632cee33ff5ba435 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001267v1_decoy LN:4544 M5:2b0987b0fb64b16ed454eaff1b74be84 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001268v1_decoy LN:4202 M5:78643a9f481cc635295ad076fe1e82bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001269v1_decoy LN:4195 M5:eb3537b9b9d637c74f1d3b43e5fd06c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001270v1_decoy LN:3807 M5:dc3d4eca4636d838ee52fe920bbffbfe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001271v1_decoy LN:3741 M5:dc2e137e3cac9a5093030c34fa68c2ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001272v1_decoy LN:3699 M5:db44a6f6dcd56edde16009cdfc186ccb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001273v1_decoy LN:3640 M5:5bda6902f082f2b4a6823836d5d0b48c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001274v1_decoy LN:3531 M5:2740aeab0828fa2d4f2789d353a40b8c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001275v1_decoy LN:3455 M5:03cdb0fa6bc0f56dc7266334229fca85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001276v1_decoy LN:3411 M5:2127857d57a589d739a30316547e9447 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001277v1_decoy LN:3387 M5:6b2e8c2f953822e034c233e94c710473 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001278v1_decoy LN:3358 M5:61be95906f76f332c029bcaaca590c79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001279v1_decoy LN:3285 M5:3c9976ca87f24507e195746c96cd0b4f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001280v1_decoy LN:3273 M5:8e25e50d38f8fb4cbe34cb3c83f14fc5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001281v1_decoy LN:3262 M5:c1e6634aa95f817084fc1e1eced2abad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001282v1_decoy LN:3259 M5:eded84ead1eb3b92edb6b77f27f8c4f6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001283v1_decoy LN:3222 M5:08c79b1d017ddd4136c42b4cb8c16e5c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001284v1_decoy LN:3127 M5:78f9666a13abcf03877f434199528e58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001285v1_decoy LN:3110 M5:19e028e80d77e35d088ea23eb52c9f68 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001286v1_decoy LN:3104 M5:7f7ac2759ee408d0324f4841b60eb208 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001287v1_decoy LN:3071 M5:cea9d8679bb3eacfb8b04f6ebbf1d4da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001288v1_decoy LN:3063 M5:52b2d8ce5acdb6c47cd5cf7924b1bc24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001289v1_decoy LN:3059 M5:8f356c7dd57f009b249e5147f708ff1a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001290v1_decoy LN:2990 M5:5ef88696d7bd42f40685d222c13e3750 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001291v1_decoy LN:2986 M5:5e39db2cc6d246fa9083dae9ffacda96 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001292v1_decoy LN:2928 M5:09a5226cdb11c4ba418a8bdf844de126 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001293v1_decoy LN:2922 M5:0b5455a2f20844d83c853abef966cffa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001294v1_decoy LN:2875 M5:82e9fdbdba365f9449c25c473beabbcd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001295v1_decoy LN:2859 M5:f0957c602b216d5ed18be6c38733aaf1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001296v1_decoy LN:2850 M5:183158b1e867bb90712701171e344b1c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001297v1_decoy LN:2813 M5:cad265dba180a6379fe3ff72f3874c51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001298v1_decoy LN:2785 M5:b2dea38e300ff000f19f2ed7c17637ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001299v1_decoy LN:2736 M5:75d2dcbfc2388d15030e0f3414952222 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001300v1_decoy LN:2688 M5:0a109d9d73d2f16494f280419a294b45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001301v1_decoy LN:2658 M5:3d83558c586ec81407c8717d1bcf07f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001302v1_decoy LN:2643 M5:c91faf6dba1b10dadf69cfb999d96386 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001303v1_decoy LN:2618 M5:10b7e187737a04307d41acec7960dffe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001304v1_decoy LN:2605 M5:3385ffd5cef2e207dd33adbc369436c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001305v1_decoy LN:2583 M5:4906d3167051868d21d54e95faccf81d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001306v1_decoy LN:2534 M5:b315ca81b05ca7fa9b615787a3b18066 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001307v1_decoy LN:2512 M5:fa1111a29692e4566683516ee2cb9829 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001308v1_decoy LN:2500 M5:51a81bda9467ea2f1691f1136a69d3a7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001309v1_decoy LN:2481 M5:66e34aaf92c2486bd4c11db6543995e6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001310v1_decoy LN:2478 M5:c4ac4ca4a4312da763c265a008380d31 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001311v1_decoy LN:2473 M5:464b136e01802236f8fcd38d3f548eb8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001312v1_decoy LN:2467 M5:b4c84cee8bcb72c5b5260d702510937f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001313v1_decoy LN:2442 M5:9d63b837b16220bf689f455bdc1d96c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001314v1_decoy LN:2430 M5:56acbbe636fd49946581fc122c6688d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001315v1_decoy LN:2417 M5:8082a8e6410572af77ebb845f8c1df94 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001316v1_decoy LN:2408 M5:9a55c9ad04d332fd58a9914422f7d836 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001317v1_decoy LN:2395 M5:c6c6115fb6941b48dc5a0abc409f5eac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001318v1_decoy LN:2352 M5:755f704f0ecbfcbbfd31d8f375c83c5b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001319v1_decoy LN:2337 M5:666a5906da91f80774c953543fb9c2c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001320v1_decoy LN:2322 M5:0f19d9949679348751b92e237ed27888 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001321v1_decoy LN:2307 M5:439c6600d966bee934ed52a2139c4687 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001322v1_decoy LN:2306 M5:27db03ab4ca9e14af33b697876aaa754 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001323v1_decoy LN:2292 M5:5fe69547ba8f4b03aaa8e71c2608fe14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001324v1_decoy LN:2271 M5:69ed4113a87f0c964d9f7515adb0dfeb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001325v1_decoy LN:2265 M5:78e3664d88395ed413c3de96d0b6aa0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001326v1_decoy LN:2260 M5:ccac0591cbfd65e9a8d51f8e05d34f2a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001327v1_decoy LN:2240 M5:bc9efb2dbf8706ae63a9da56f74a5940 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001328v1_decoy LN:2238 M5:a0265d5b1e9532d8f124c363fe675206 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001329v1_decoy LN:2228 M5:422f2cb811b961c3513bb155a4a0fed0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001330v1_decoy LN:2215 M5:0d7e0facbdb02ac5cbe36bcb3f1683a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001331v1_decoy LN:2205 M5:8aeb5e1e49ad042e60fd97cbe3bd36b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001332v1_decoy LN:2191 M5:bc35b989cffe895e99e6a2fc45b7f65e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001333v1_decoy LN:2191 M5:b1ccfad8f6bde8d9d40c0b7199e30cc5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001334v1_decoy LN:2190 M5:0ae32cfb78573ddb9929745e2e7a0313 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001335v1_decoy LN:2184 M5:08a18a9ad801c228f5de34b35b58ce5f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001336v1_decoy LN:2166 M5:95badad6f81c843868ebb07d1f2d7911 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001337v1_decoy LN:2165 M5:e10a87e41dccb3ec25e99df332d5a560 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001338v1_decoy LN:2162 M5:7973da1851b09e72b47bf83450c468dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001339v1_decoy LN:2146 M5:e8939fd820d746a8792b356c04e54cce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001340v1_decoy LN:2116 M5:b67c09bdf1ed2f6a0ddd972711b36104 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001341v1_decoy LN:2112 M5:90340b9fb118df304042bb73e83ec202 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001342v1_decoy LN:2108 M5:465277293cdb0bf1897d5a17e266d354 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001343v1_decoy LN:2106 M5:ddc350523aba6f1c4b90f820904cd233 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001344v1_decoy LN:2106 M5:8c726624d2d1347ec079d4a89d25759e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001345v1_decoy LN:2106 M5:4b1887bbf69131a205c5cc1a4303c860 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001346v1_decoy LN:2097 M5:6dc99a12532b35dedef60dc2cfae35b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001347v1_decoy LN:2081 M5:12316554f4811e33853508ac262465d6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001348v1_decoy LN:2058 M5:b1935843fbea95bed6588d9d700d9936 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001349v1_decoy LN:2055 M5:b013f57c784235eb32f6871dd01e5e13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001350v1_decoy LN:2054 M5:c6835412045762a4c54200ada23f30b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001351v1_decoy LN:2037 M5:50c1358c2b24fd59d5a286bbaa57818b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001352v1_decoy LN:2032 M5:45ee3ea9d4de3859645f9b51f0ca2c67 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001353v1_decoy LN:2032 M5:924e5cc3295232068cf32022f5f7e779 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001354v1_decoy LN:2020 M5:70660abb35fad19ab75c06b301d4163a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001355v1_decoy LN:2018 M5:9126be7c6d2612a65885a2fec496e2a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001356v1_decoy LN:2014 M5:847a7ff01c6682c63ad35fae4dd6f947 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001357v1_decoy LN:2001 M5:71668bc6a710ff919fd32549ee63f1f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001358v1_decoy LN:2001 M5:3288f97535eb71395eaf3b088578bc3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001359v1_decoy LN:1991 M5:b29d731dd150421c4e201d1adc92bd3f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001360v1_decoy LN:1990 M5:7e91b8bc1cf2c754f8af12a28921575f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001361v1_decoy LN:1983 M5:71f21061b64aff6e62b116e9384a099a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001362v1_decoy LN:1981 M5:87dba9523601c5cfc29630be31297114 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001363v1_decoy LN:1981 M5:2a3b4956ecd118211708b9d7c428818f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001364v1_decoy LN:1979 M5:eb8501b5022cd696a32a2bff2acbbb3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001365v1_decoy LN:1963 M5:87a0635cbf78d7359208e0d29a3b9596 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001366v1_decoy LN:1932 M5:42a66b3579061c6333cfeec8e7bc7b0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001367v1_decoy LN:1929 M5:95db3317d465876b900ca9167ec31b73 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001368v1_decoy LN:1881 M5:31fb7324b8669148c367ef667ff3c928 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001369v1_decoy LN:1874 M5:bb9c6a2d6ec56835ea520c45a854e8eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001370v1_decoy LN:1849 M5:8f83bc8528784a67dd64f10d89331d81 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001371v1_decoy LN:1849 M5:aadeed33b6077b0febbd1f61c186805c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001372v1_decoy LN:1833 M5:155c69774a39a66871981c66bb0f0535 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001373v1_decoy LN:1832 M5:2677f37d4424e092deaaf6c2371defb9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001374v1_decoy LN:1826 M5:4dbca21467f25692d20f062b08c3238d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001375v1_decoy LN:1814 M5:208039e11c53bf03a11d83b417fe5920 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001376v1_decoy LN:1814 M5:8ef785587310c1f2d2226b740e7e4553 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001377v1_decoy LN:1791 M5:ab8270aa2cc7626669c77a954b338953 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001378v1_decoy LN:1789 M5:3ac762e7071f807dda31f5513d9624cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001379v1_decoy LN:1786 M5:9149be02831b6a76a57da93d45460920 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001380v1_decoy LN:1778 M5:a17c5b0d2e1771a62091b6705cfbf5a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001381v1_decoy LN:1776 M5:2ebb9adddc8d21f56ed91c6fbc97cfe1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001382v1_decoy LN:1762 M5:686009ce9e6490069ba0845173cf2c16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001383v1_decoy LN:1758 M5:34dc7958ca4e0be1690c80cce1f048de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001384v1_decoy LN:1757 M5:af98ed1ffd7c4a0166ed1d3bb789d039 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001385v1_decoy LN:1754 M5:80b9896356b85bbe70c7ed49afb616a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001386v1_decoy LN:1752 M5:a7c233ed3f050d44d75a9138231b5384 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001387v1_decoy LN:1751 M5:6e2fe9e7a9951deedb4a39fd837721a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001388v1_decoy LN:1749 M5:5d5cf3896254f422f70ace465b0f820d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001389v1_decoy LN:1738 M5:f8265cae7fb03630effc0fede6811b3f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001390v1_decoy LN:1729 M5:48fe4b417655ad69a29770ec6e412fdb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001391v1_decoy LN:1726 M5:01bbe99249b489660daa17fa9f958d10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001392v1_decoy LN:1716 M5:bd9a3ab49b9960e88c2490efe5d8c749 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001393v1_decoy LN:1712 M5:f05c2bfa07a617b06e6a8ebc4efc5022 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001394v1_decoy LN:1711 M5:0f06abd7c321228fa763fb3815140af5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001395v1_decoy LN:1703 M5:09b91ffc2209fd9adc0e0fbac86de4bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001396v1_decoy LN:1702 M5:ff652624b7319ee60f8946d8f751cf16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001397v1_decoy LN:1699 M5:2a71713d93ad2a6a8edc736ddc02907d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001398v1_decoy LN:1686 M5:3bfbf7d5273c5ec692b1907c7c3ff2d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001399v1_decoy LN:1684 M5:6d040733a43c01650e5c098e00d46a62 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001400v1_decoy LN:1680 M5:c353280f0d833806daec83385e95e393 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001401v1_decoy LN:1678 M5:c2edb1379f817a8378d4eb089dcdfc0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001402v1_decoy LN:1678 M5:740b37d2e6785fc699776f93cc872e86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001403v1_decoy LN:1677 M5:6d4b39914847504188d3b9feaf3348fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001404v1_decoy LN:1676 M5:31845da6d4ed6000f50d5122bdef15cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001405v1_decoy LN:1672 M5:0630c8622e65da828a153d66ab29b298 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001406v1_decoy LN:1669 M5:e7d237d72fb65c19aac7bcaca3d86d95 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001407v1_decoy LN:1668 M5:86c9c0f792de2f54f69278df5e3246cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001408v1_decoy LN:1663 M5:481fc7b87114d19302cdde760018a39c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001409v1_decoy LN:1660 M5:ca249c982b9bc0cae8d02ae5a7e497cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001410v1_decoy LN:1660 M5:9a4adc64cf2fd32db34af21df269bfe0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001411v1_decoy LN:1658 M5:9e0ca112ba9edd83dd02ee0a1b977682 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001412v1_decoy LN:1656 M5:30ba767a8335a19ffbb875f109afcac3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001413v1_decoy LN:1656 M5:e77633b957a375847ad25f9b01d08580 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001414v1_decoy LN:1652 M5:33a7cad76ee390036b7d61b167e2b351 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001415v1_decoy LN:1647 M5:904ad5deeeb540a10728c9fabf9e5909 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001416v1_decoy LN:1645 M5:378193508f34a25cf912974a4c531bb2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001417v1_decoy LN:1641 M5:fe667f1d920a30b730d27903d4de3610 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001418v1_decoy LN:1638 M5:dd1f1f8b342e987caf5e43ffa4b193ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001419v1_decoy LN:1633 M5:775a347b6935f9e170d809222897833a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001420v1_decoy LN:1626 M5:44b1b569182bce231346586fe77f0651 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001421v1_decoy LN:1614 M5:04a70773f36888dde7766a58c3a92de1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001422v1_decoy LN:1612 M5:0f0f7a8906ae3367b908acc806579e87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001423v1_decoy LN:1605 M5:345b541ac9d588f32be39d15d00f6305 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001424v1_decoy LN:1603 M5:dd25e42a5806fade892f5be6c5517133 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001425v1_decoy LN:1599 M5:42c61caf5957d89e892870d7abb0086a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001426v1_decoy LN:1589 M5:ff1c151f692dba0e79005392390f2639 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001427v1_decoy LN:1588 M5:d409b0b1699f38489ac9b78c9e547b12 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001428v1_decoy LN:1585 M5:549a7c1ad8b79d31a311204a8ce078c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001429v1_decoy LN:1584 M5:21ac82f5d3944ac17a667861fe4eb50c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001430v1_decoy LN:1584 M5:d717e9b20496c0e3cf6f096572904e94 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001431v1_decoy LN:1580 M5:4cef86e6f0c85a15667ecccf0ec2e509 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001432v1_decoy LN:1572 M5:83a76ba3b28ab176a0fe0c3932129c1d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001433v1_decoy LN:1570 M5:eefbc920c0c274a05ccd03672089bd6a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001434v1_decoy LN:1569 M5:e84f6544b2757c3d68e8333237c05b9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001435v1_decoy LN:1568 M5:085a737d9ac2ce5ad615c127373713e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001436v1_decoy LN:1567 M5:d0cc61096796d51c565e73784c3df91e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001437v1_decoy LN:1565 M5:963645d93472e0f55f70cf30e5541dcb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001438v1_decoy LN:1559 M5:6cd647a043602392b10b564ef25b9bfa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001439v1_decoy LN:1559 M5:41e3d96f1e4d9a77d4314fb0c550ddc8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001440v1_decoy LN:1556 M5:f95112abd208efc5ebe4d5529e2537a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001441v1_decoy LN:1554 M5:ff55b1b730eb936e0ffca09b8fff9a89 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001442v1_decoy LN:1549 M5:df38873f39fd89b3782d53eb4bd35345 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001443v1_decoy LN:1542 M5:66b6405dd4c7537d3119eeb1dfa81795 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001444v1_decoy LN:1541 M5:ce00bd7a73eab91b6d81729240662ea1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001445v1_decoy LN:1538 M5:96e6001e127eea7213d8378afb6f24d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001446v1_decoy LN:1537 M5:4a49340b952f5a6e1fbcfbe271cbee49 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001447v1_decoy LN:1535 M5:9d30511ff7644e5e501c629d82f97441 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001448v1_decoy LN:1530 M5:ed3d23f4c537a88a36a4a6964bf2d332 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001449v1_decoy LN:1528 M5:e2f85fa47e8361a098aacb0ddfe058e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001450v1_decoy LN:1522 M5:525443cf312658767f3ffb263b1ab40a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001451v1_decoy LN:1514 M5:d378e3b3268d04ee2ed255d9b671404b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001452v1_decoy LN:1509 M5:434a0e69d1dfb2f439fd8c4c6efee812 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001453v1_decoy LN:1507 M5:1c60f6588a6a6fdf49ba74bd4d8844ae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001454v1_decoy LN:1500 M5:4a8517743aa87c3edd4556e876facf8e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001455v1_decoy LN:1499 M5:3787c00e3019dc0bd4b019ac8bd677e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001456v1_decoy LN:1499 M5:93e8bbddc43d5e902eaac3b1569c191d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001457v1_decoy LN:1497 M5:cedf6613fedd7bcfb6cf4822d6133393 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001458v1_decoy LN:1496 M5:c6421744963f5f5ef7d06349345f8c11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001459v1_decoy LN:1488 M5:c3d6f0be5c1768c3f4ab357b40330c07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001460v1_decoy LN:1486 M5:cfa272f94abe2b6ea6b8fff9bc2743a0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001461v1_decoy LN:1485 M5:73b05431178ff099c7143b2c3310a8d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001462v1_decoy LN:1481 M5:815cd718fd3524c82596f553bc50c030 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001463v1_decoy LN:1479 M5:3be54176b83f28ddb8402da62d8368c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001464v1_decoy LN:1472 M5:a3d5a1d61c0377010cab585d8f86782b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001465v1_decoy LN:1472 M5:54ed54ed3b16b9482b8bb7a42358e3f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001466v1_decoy LN:1470 M5:957a8cadb7ce3ea0f910acb49b0d28f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001467v1_decoy LN:1466 M5:5f1e18ba047f8e50f57be2e0ec9d7ee0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001468v1_decoy LN:1465 M5:1371b580bc13e98f593106d0c6cc7055 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001469v1_decoy LN:1461 M5:a1e27c8891fb421149591b6254008534 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001470v1_decoy LN:1458 M5:0a299df79227b0b8f9d01b64c1091040 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001471v1_decoy LN:1457 M5:fb81cfbfe3dcf83480d1566dbfb35cdf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001472v1_decoy LN:1448 M5:5ccf4c226bbe31dec446e5f2cfb754aa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001473v1_decoy LN:1447 M5:d4e733a5240b77f6521178683ef92f11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001474v1_decoy LN:1444 M5:266aac36179cb1faca5c4a0102fc8ace UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001475v1_decoy LN:1443 M5:6153fa9f83b2dca77912302a435e6783 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001476v1_decoy LN:1443 M5:eadd62062090a328419de772556bec04 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001477v1_decoy LN:1438 M5:808b663863cc7b8062a075d60a932ee0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001478v1_decoy LN:1432 M5:3d320a29f829d8b30da651831563b8fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001479v1_decoy LN:1430 M5:d406932c6d2e5cda5d40bd5282612ec8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001480v1_decoy LN:1430 M5:1b514b0604012b9c3bf96dc729d5b570 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001481v1_decoy LN:1429 M5:bba1c32000bf8515723b23e91e3d73d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001482v1_decoy LN:1429 M5:469a525eaccd114705d3876e6497d24c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001483v1_decoy LN:1429 M5:1cab4c8d35aa87cfadcc67327c0cde05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001484v1_decoy LN:1426 M5:7f0b3e31f88382eb1a9d667b4fa5ae8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001485v1_decoy LN:1426 M5:600029364a5faff2a6121c791c26f3b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001486v1_decoy LN:1420 M5:bd2635b8f37ae8d36555126a2efda82f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001487v1_decoy LN:1416 M5:1feda0b1dfda8eb29e804bb83d191350 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001488v1_decoy LN:1416 M5:381713228fbc436ef15eb3fc5ce95526 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001489v1_decoy LN:1415 M5:c7b5a3592ce2955180a8bf93340ce7a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001490v1_decoy LN:1415 M5:8a9071712ed09640282279375c1aab9e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001491v1_decoy LN:1414 M5:ad3849a6c284e0371da519c83c3efd56 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001492v1_decoy LN:1413 M5:1897ebbd3ccc3ed5dfd33e64df6cee2e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001493v1_decoy LN:1410 M5:bbae6ade32694ea4d23d50d382e589ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001494v1_decoy LN:1405 M5:60eac2719276e2c8aed1dd8879086460 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001495v1_decoy LN:1402 M5:560d0b9e5abc45feb134351a94cda358 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001496v1_decoy LN:1398 M5:c87eb09186b0ed776438697cefe49c34 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001497v1_decoy LN:1397 M5:17bdfaf46a6963ba97861fcb9ff9d4ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001498v1_decoy LN:1395 M5:14942812a5a15869999430aa17472a47 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001499v1_decoy LN:1392 M5:14d8c0e42dbe62d45491f288a844c263 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001500v1_decoy LN:1388 M5:3535c9f286c4bc12e2fdab3ebd0e4cbd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001501v1_decoy LN:1386 M5:3ed31b85ef3e34d8e053031462c9cc64 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001502v1_decoy LN:1382 M5:370ef584d4cfe29bba430512c01c2d92 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001503v1_decoy LN:1381 M5:0d6317f9e8c37b0d588aaf5c404621d6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001504v1_decoy LN:1379 M5:15b371e04aee1e2cd7a2c3ad778615cd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001505v1_decoy LN:1376 M5:9e5d443eae0beb889e93d9fec1df500f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001506v1_decoy LN:1374 M5:19e75c42356ec21ffffd6ead1147246d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001507v1_decoy LN:1374 M5:d047841fe73e626cbad1d1a075cc1179 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001508v1_decoy LN:1373 M5:9af5c2bfca12c6e109d87d15a001f382 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001509v1_decoy LN:1373 M5:26c1cdc503f21321bdbbf7184681fd57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001510v1_decoy LN:1372 M5:c08f8502b7b9423df1c4988d25d4e1e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001511v1_decoy LN:1370 M5:51b5c0febc1d93a3ae056b562f29febe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001512v1_decoy LN:1367 M5:84cb97f5fa89889026682a6ddb4e17ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001513v1_decoy LN:1365 M5:9b35f302f4d7104621ee94c9466fcf05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001514v1_decoy LN:1364 M5:d26988a458e184ce67dfdf1494cce818 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001515v1_decoy LN:1361 M5:2be502a9c104f048b5faab19bf6b0c94 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001516v1_decoy LN:1361 M5:a66a14b66c130258ed0f9a2e60b977a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001517v1_decoy LN:1355 M5:f8520c48ea6b728718603ce85ba18832 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001518v1_decoy LN:1355 M5:d16784b4fc04cab4570de94e9753760e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001519v1_decoy LN:1354 M5:226153c1ffcb6ca5cb3a64d124c3b9fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001520v1_decoy LN:1353 M5:36898b58e8fec2ff86a3db37bffcfe2d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001521v1_decoy LN:1349 M5:919f3dc576e969ebb845489e155befd1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001522v1_decoy LN:1345 M5:e1f3dc21c137a83319e95d8cd641953b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001523v1_decoy LN:1344 M5:c7cc4c4587e7e5f69d3afcaca3f62ef6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001524v1_decoy LN:1343 M5:12b492bc7285ab647a13d8ac1efecc61 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001525v1_decoy LN:1338 M5:8d831cbc5a7d72f9a5c05360b502005d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001526v1_decoy LN:1338 M5:89ce2ba0c1414088ff5f502a0908059f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001527v1_decoy LN:1338 M5:ed5c2d0150ddb784f059ca25eb3b32c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001528v1_decoy LN:1336 M5:1dbe3107f4bae73b01853e98084f8f47 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001529v1_decoy LN:1333 M5:1d1e9eb7e4182425f8718dc456a8e9bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001530v1_decoy LN:1333 M5:d77e50f1375e757aefd872040ee290b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001531v1_decoy LN:1332 M5:93546f6ce6b44646b5a2d20190054dcb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001532v1_decoy LN:1324 M5:bcb215dd97bfb4682fe3d17f99605c5f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001533v1_decoy LN:1323 M5:0c68586edd47761d55b6a127af21b958 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001534v1_decoy LN:1323 M5:8290481e03f0efd3da641df90d6a09f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001535v1_decoy LN:1320 M5:06b5a586c070a38af486a0ae25dac08e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001536v1_decoy LN:1320 M5:aa7f4ccf5b8955783f201e89c6b8b61b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001537v1_decoy LN:1317 M5:5f5c4304240e41a75cdf79d590705a7b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001538v1_decoy LN:1316 M5:2b2e47e474a04b2c5d7538dee74a4520 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001539v1_decoy LN:1304 M5:70b1f3865cf28cf8c3cb6ca89e299e75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001540v1_decoy LN:1304 M5:801a2a35c0eb1ddde4f339fae91bdd02 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001541v1_decoy LN:1303 M5:0013745a3b7f3937b3f0d58b8fb8b96d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001542v1_decoy LN:1302 M5:2e708be5a88bc87556f10487d43af5f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001543v1_decoy LN:1301 M5:9b421120b8e61665a80c2202f9742b11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001544v1_decoy LN:1300 M5:7b6b985d4c9b335dde3369fc8ae75a4f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001545v1_decoy LN:1298 M5:c2c9f5a874990de1d53e53efca362cd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001546v1_decoy LN:1297 M5:8849c9f185b5ae8ed6d60d3b99c6591c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001547v1_decoy LN:1295 M5:134596128a40e4a4c8f40d310152fe2f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001548v1_decoy LN:1284 M5:c943fc593d7c12a3edad870f24db083c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001549v1_decoy LN:1283 M5:de6680db4756a0d685d2f2405be073be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001550v1_decoy LN:1283 M5:a86669f9a78babae5db999b036d712b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001551v1_decoy LN:1279 M5:48d3b7e152913253a1c7749be4542da0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001552v1_decoy LN:1278 M5:6f732db21b1679f66b34145d0d179a57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001553v1_decoy LN:1271 M5:18a0c55016d63414e1a7c9a868d2167b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001554v1_decoy LN:1271 M5:fbb66e8da3ee67360e823061c517c47b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001555v1_decoy LN:1268 M5:668bc50dfcee6830bfd6b8b193cf9aab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001556v1_decoy LN:1264 M5:512e1b52aa8bc91a79e98a21f48fd69a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001557v1_decoy LN:1263 M5:a280a5ddd14400e7a21e262dacba0878 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001558v1_decoy LN:1262 M5:074796c17da27a3ae23b3ab43837eff9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001559v1_decoy LN:1261 M5:f75ff3300dbd75160e935cd46e4511c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001560v1_decoy LN:1260 M5:67884b5403ffc61f67795ef0fcc3b616 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001561v1_decoy LN:1259 M5:778ef716a17f44127ce4648ca3d01437 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001562v1_decoy LN:1259 M5:79561487702e7f87d4c1babb97cbf910 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001563v1_decoy LN:1258 M5:05c2d3d1fe5c9569c6f982e7978b0d6d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001564v1_decoy LN:1256 M5:443af4844c7fe05604fbf14d61807faf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001565v1_decoy LN:1253 M5:3ab1b0cbc9dfe5e1b97cc5bbee6ba5d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001566v1_decoy LN:1248 M5:6ca9712ce96adea0a2a178a885e6c403 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001567v1_decoy LN:1248 M5:a9815826b47ba4c29bb0b6f427c91f9b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001568v1_decoy LN:1246 M5:847154b1d516e188b5d8b9b6932658a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001569v1_decoy LN:1246 M5:cc8ad8f95e7d72b075ce88fc9fd93560 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001570v1_decoy LN:1244 M5:3df737516c175d1baf5cb57874761a06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001571v1_decoy LN:1238 M5:350c62d6852c839c79bb94399823a7c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001572v1_decoy LN:1238 M5:cf4520e99994e5810535b8e92f1f4764 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001573v1_decoy LN:1236 M5:27e61fe700513169fa02b0a6b9224fb6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001574v1_decoy LN:1234 M5:6af09c7c1feeb50a156ab599d39314f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001575v1_decoy LN:1234 M5:1b1c2b7e28c781322697a77df6b717c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001576v1_decoy LN:1231 M5:24168a14a8a7b0626a75964a5ffa4c06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001577v1_decoy LN:1231 M5:7b5c30f4c44dc17f05a6022490a1b06b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001578v1_decoy LN:1230 M5:7dd3d8aa3ad6ae0467cbb5729a65b7fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001579v1_decoy LN:1230 M5:9838f95ca9d84335f580003e43e0e909 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001580v1_decoy LN:1228 M5:40d2f9b81666f0aeaadf3b60d6f00326 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001581v1_decoy LN:1227 M5:b603baea2a6d239b5a0e0482be39ebae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001582v1_decoy LN:1222 M5:592ec40093cfd07fe7ae22531e7ff948 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001583v1_decoy LN:1222 M5:45d2796dafa85ef3ebb8d378ab5c4fe2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001584v1_decoy LN:1221 M5:7171fedcff845d9bf0eed62d8de65582 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001585v1_decoy LN:1221 M5:1919b7c47b361c4925ba7f89f672c639 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001586v1_decoy LN:1220 M5:9b6bee47fab2e16188e112fd219cb63b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001587v1_decoy LN:1218 M5:403153f244f25427f95b761c7e7643f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001588v1_decoy LN:1218 M5:4bdec42af039130bb647f35a1587442e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001589v1_decoy LN:1216 M5:5eb119c85dbdbac20fd9b54ca19e3bb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001590v1_decoy LN:1216 M5:30be84f72824c6a73cb46db0880fca34 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001591v1_decoy LN:1212 M5:a3990c8502d89fd44d2744a7c3b5ec27 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001592v1_decoy LN:1210 M5:812ecd7a45c18a1ca8e07d380fa9c7fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001593v1_decoy LN:1209 M5:f3658490a61b9678c62147607e15dd39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001594v1_decoy LN:1208 M5:6ad7956bf60e17b114d96c186021ba22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001595v1_decoy LN:1208 M5:07b45f43574a98e35df01f1b02658afa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001596v1_decoy LN:1206 M5:7ad1a707f9ee83f188f836b84f0ee5de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001597v1_decoy LN:1205 M5:c5fe9e310df6cae87f2d597153392ce3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001598v1_decoy LN:1205 M5:3253a26afa899ec2cde4d0fe80a95557 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001599v1_decoy LN:1202 M5:7eba2ca9a5450bf7c6febae054f41555 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001600v1_decoy LN:1200 M5:00f180d0f4f2be5d3a79232ebf1f4150 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001601v1_decoy LN:1199 M5:06c81b1f3014d187114393c990dbb3d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001602v1_decoy LN:1198 M5:04ad661ad55cf80b26d65b537fa28426 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001603v1_decoy LN:1198 M5:10cec3a4b27c1a9e83dba32c764db1a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001604v1_decoy LN:1198 M5:ca938a555284768f7de4aa5bc45030af UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001605v1_decoy LN:1195 M5:6352f3ee72fc500fb234cefdc58d230c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001606v1_decoy LN:1194 M5:7761a04b81c344864c4196c9fa348529 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001607v1_decoy LN:1191 M5:6f495eb0c24a06b146428c8e15145289 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001608v1_decoy LN:1189 M5:7cdef7e7fe5039c26f91054002844a08 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001609v1_decoy LN:1188 M5:8fb25530ab0d6cc405ed3820a00a19b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001610v1_decoy LN:1180 M5:baa36ab5e7d359dcf38de58e0578aa06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001611v1_decoy LN:1180 M5:fbf128a1dc1f9852e12d0f7cc819e220 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001612v1_decoy LN:1179 M5:cb72123adfc2996fb4f8c8023e656377 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001613v1_decoy LN:1172 M5:9a828a68ee9904074c07ecb9b4a66d85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001614v1_decoy LN:1168 M5:47f21c445bb5e0f2901a60313a4b54fe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001615v1_decoy LN:1166 M5:6640bdaa008c2b633f8732710aea42e5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001616v1_decoy LN:1157 M5:548cacc169d84926fc794c75c8fe890a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001617v1_decoy LN:1156 M5:ed240ec48dfc49536a05c15b0cf84b32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001618v1_decoy LN:1156 M5:4cf17295af5ac519eeb5b60d65d8d630 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001619v1_decoy LN:1155 M5:7535ed3093386510b39164d7d8c0e75d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001620v1_decoy LN:1154 M5:942a2c1ebc2dcdc44aca71c7998704e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001621v1_decoy LN:1154 M5:172266936a16cb96c0fa51a30de2f617 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001622v1_decoy LN:1149 M5:904c6976a4151582f6e20888f8882b1c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001623v1_decoy LN:1143 M5:e146ce9a62548893f8042eb31c9eb73a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001624v1_decoy LN:1143 M5:226937ae596ef9af068232903d42c1f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001625v1_decoy LN:1140 M5:9f8924f0407c98c271d938a89ce0651a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001626v1_decoy LN:1137 M5:e6dc97de94f6ebca61e2afaa11081a24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001627v1_decoy LN:1135 M5:ddc4671e6fc9af479ecc987b65228b89 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001628v1_decoy LN:1135 M5:156395c17267dbc279afab948e04d7eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001629v1_decoy LN:1135 M5:b51c39a7e5a4d0f3a185dfe0405d28be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001630v1_decoy LN:1127 M5:eee6cec0d44e913c8909412bdc06fa14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001631v1_decoy LN:1127 M5:e614e251417f03894a86d226643c44b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001632v1_decoy LN:1126 M5:e3d6bcc05df478652842790b2163c80e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001633v1_decoy LN:1123 M5:62a0c3d0311f689e83c69aca70ff1d7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001634v1_decoy LN:1123 M5:4c25a0253e0d7c0aa032a2af4dc159a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001635v1_decoy LN:1123 M5:77f17c8bda201de22cb683529469d159 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001636v1_decoy LN:1122 M5:4716e9809cd6983cc872a801adcfcc46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001637v1_decoy LN:1122 M5:e854813fe411b829feb5122630115359 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001638v1_decoy LN:1121 M5:9ee360ada2996f37f05bc35f51af2274 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001639v1_decoy LN:1121 M5:e9eacf65a91b75a7484d73c0960d1f14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001640v1_decoy LN:1119 M5:115a5a8bf67d2676b18db1fa2cf1fb41 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001641v1_decoy LN:1119 M5:8c12171780de61fb413546e612810679 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001642v1_decoy LN:1119 M5:d36511233c4008330a6ea48f39a48c8e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001643v1_decoy LN:1118 M5:49d64211fa0e9269ff0335c106fadf0a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001644v1_decoy LN:1115 M5:d3b2472895f28b7fa60956f7b18c525d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001645v1_decoy LN:1106 M5:14d31945977d0566bc15d63216affa59 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001646v1_decoy LN:1106 M5:954d5725f47430e77ac7a65db6252cb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001647v1_decoy LN:1104 M5:afc20c7775696bfe6b465aa9d8d5a2cb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001648v1_decoy LN:1102 M5:1a699dde289aa62b55f6ac11bb7947e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001649v1_decoy LN:1101 M5:97d8bdd4c01947f81c47b798baa7b71e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001650v1_decoy LN:1098 M5:81be16ab671a23dae662d52a90f53d45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001651v1_decoy LN:1098 M5:849d2fca45af7dd15c549778da256bd5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001652v1_decoy LN:1096 M5:85135079f59c18f32cab3330238996f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001653v1_decoy LN:1096 M5:eeee606c5ce1877c2c17f3c35cee97c0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001654v1_decoy LN:1095 M5:a67b439018f9500bd22116207fa78bb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001655v1_decoy LN:1093 M5:45193d6dc28c575afe0a816a04981a13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001656v1_decoy LN:1090 M5:9e9a3deb3fbb62096e9267cf17b4538e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001657v1_decoy LN:1089 M5:2fcd006deec28bc7b3e2ed1c81f6bd69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001658v1_decoy LN:1087 M5:b5ff50152c9932bd5f5c8f6d8f085250 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001659v1_decoy LN:1087 M5:8dc8692a004984e174cae1a798c308ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001660v1_decoy LN:1085 M5:a26ef2777b049efc475e9f983166c858 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001661v1_decoy LN:1085 M5:1d1b538881461819bddec7da548477d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001662v1_decoy LN:1085 M5:a02c26203237d3ab0fdeec3b2226e415 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001663v1_decoy LN:1083 M5:97595ecd8251d05c23a9a1f24c4fc331 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001664v1_decoy LN:1080 M5:bb065f6218181f056b36deb6d5ea3fcf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001665v1_decoy LN:1080 M5:242177401fdbd05a55373745dd7678ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001666v1_decoy LN:1079 M5:aee38ae173783e327db2c83e4636269b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001667v1_decoy LN:1079 M5:eb281bc766d87e3e887aff0c1f473bb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001668v1_decoy LN:1079 M5:b15b982e045772eb4fae4cf61eff32f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001669v1_decoy LN:1075 M5:a0fe4aa8f27e7528a49b7fc241413bcd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001670v1_decoy LN:1074 M5:5a9ee12d6200281af569684e9eb6c44d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001671v1_decoy LN:1073 M5:58e3e1c01c24005c1bf6ecc4081328af UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001672v1_decoy LN:1070 M5:6b4eaaed019b55c1445f856fa75748c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001673v1_decoy LN:1068 M5:962507e31477075a1ec295c7f3e4f370 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001674v1_decoy LN:1067 M5:246916ebbed387cdb53894261342b334 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001675v1_decoy LN:1066 M5:45ef97be51801b68eddaf9d903c6569e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001676v1_decoy LN:1066 M5:a1e2a77d7c51fc62c3f1261d96788416 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001677v1_decoy LN:1066 M5:a72df1625ec9a3676769041db2b5db74 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001678v1_decoy LN:1063 M5:63c3ca073be59c038fca2ce8cf57b58f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001679v1_decoy LN:1063 M5:3b4176334e519bf0fc0ea90b5c450256 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001680v1_decoy LN:1063 M5:e0c561be692e2103d216fec5f81a004f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001681v1_decoy LN:1062 M5:a87bd7c0b225c0db2cc82df0e92fd34d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001682v1_decoy LN:1058 M5:bd1fa296d60d2c1447479727889fd06d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001683v1_decoy LN:1056 M5:ec709ad9663274dc4b8d9c95637e6231 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001684v1_decoy LN:1052 M5:4c92b0e2cf0179f74e829358fdd4fd7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001685v1_decoy LN:1051 M5:d301a97b1b34b2cbcd690824430ef873 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001686v1_decoy LN:1051 M5:bbc47709fbd21b2776773a149cc0338a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001687v1_decoy LN:1050 M5:a01a1af5e1a2b7e924af29af696e561c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001688v1_decoy LN:1048 M5:71b188e0ec08d62bcf1e70c1ad5dece1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001689v1_decoy LN:1046 M5:6a0e08e5268e2e656a415ba75e6fd9bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001690v1_decoy LN:1046 M5:59e6b5101b057b8b61117d4e775da1a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001691v1_decoy LN:1045 M5:f09e7737756f026981c00c9f4e4a0fcb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001692v1_decoy LN:1043 M5:90cc154008842d098dcd3bfcb288fad9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001693v1_decoy LN:1038 M5:760fd011482ffc7b2972fff26ede08ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001694v1_decoy LN:1036 M5:403a116827eda12cce96c83a442432ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001695v1_decoy LN:1035 M5:aa903a22959e0e71bf9b65e184c98664 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001696v1_decoy LN:1035 M5:c7b013db629c9e53bcc9e5ef6b45ea86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001697v1_decoy LN:1035 M5:b124dac1b39002796762805b01e70939 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001698v1_decoy LN:1033 M5:973b579d06cff1e48883e0c933a40ace UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001699v1_decoy LN:1032 M5:d91e5694037129aa77037d5c631b2c89 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001700v1_decoy LN:1031 M5:26f0a1a25d70795beec266b79ee9872a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001701v1_decoy LN:1026 M5:d895a2e531136b85f91cae9c41bac3eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001702v1_decoy LN:1026 M5:eeaed2987f143706ea90f593db2ee43c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001703v1_decoy LN:1026 M5:201ae22e581315eb83bf1b2a90a376ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001704v1_decoy LN:1023 M5:676723aca3cbe94f5e6fa6e3e677382e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001705v1_decoy LN:1022 M5:079f24ae7a37258e2dcd3e45c2a82dc0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001706v1_decoy LN:1020 M5:0c6c76de07c4a66f7b7c1625990fe97c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001707v1_decoy LN:1020 M5:23b7cef7e53c021320be37e395337a6b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001708v1_decoy LN:1020 M5:442bbaf4d63d7243f581f2a4f3c8713e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001709v1_decoy LN:1019 M5:ead84759fc3d0742886397aa847a8c43 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001710v1_decoy LN:1018 M5:30842e0e30b9626a2db87c8abee99c69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001711v1_decoy LN:1018 M5:85cd9b0b4ac27a135417acad1b49f347 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001712v1_decoy LN:1017 M5:cf02f8f140f6cabc6edaa85f110e6a45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001713v1_decoy LN:1015 M5:a1b077bd6e18faf0ce248051997e87b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001714v1_decoy LN:1015 M5:c108897419126d2b77335084625f756f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001715v1_decoy LN:1015 M5:887e1695f416731637dc46a35b2a9548 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001716v1_decoy LN:1014 M5:f4c888663c13ab55190b9228c5b46bb8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001717v1_decoy LN:1014 M5:be3c4d0d37c5c1c8c88889dede5ba4b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001718v1_decoy LN:1013 M5:2d3c3dfc692750b9b4c716debe8451a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001719v1_decoy LN:1013 M5:c1212f7ba1d00f6f6553fc075e858e42 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001720v1_decoy LN:1013 M5:6e854869ecc63cf6e45e7f0fc6eeb9c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001721v1_decoy LN:1012 M5:05b1945756fa1adf0d4547bcd12f01ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001722v1_decoy LN:1011 M5:0ef4eb4aa59e9725d43ed27a2f67d8d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001723v1_decoy LN:1011 M5:b4080ef5ca76b971d7eea5438484ce22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001724v1_decoy LN:1009 M5:34953db0af28061b89bb32262121af7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001725v1_decoy LN:1008 M5:b05c20cc6394423a0e1aef1bac72d0b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001726v1_decoy LN:1008 M5:f2301601e3a88c6be9c38500a9d29685 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001727v1_decoy LN:1007 M5:2ff7ac77e3568eeb2e7772e932dfa819 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001728v1_decoy LN:1007 M5:04fd5dc748b4e7815316b0cccea3cba0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001729v1_decoy LN:1007 M5:957456ba0e3f879860817f3ae6bfca78 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001730v1_decoy LN:1006 M5:8381668c661d255ffbd7498d663acff2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001731v1_decoy LN:1005 M5:92a03e55b43fc40c9c010df225cc650c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001732v1_decoy LN:1003 M5:bc99a37cda9c755b77763c0873a3435d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001733v1_decoy LN:1001 M5:6a123c9c76f9d1c0a35ba3666f861ddd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001734v1_decoy LN:1000 M5:31048d43d8cae8a2c51fe5fcfbfa2462 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001735v1_decoy LN:19311 M5:110c68ef04650368b85e76c55d599fcb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001736v1_decoy LN:11713 M5:392799b68e6e2c6e288f07bc44a3a6b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001737v1_decoy LN:11263 M5:091c74a89bf6767adbc92c7cfd9ab6bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001738v1_decoy LN:9779 M5:fdbeb36b87e966517a30478e8fd55631 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001739v1_decoy LN:9568 M5:3562fa1ff9f4f23377f715895ecd504e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001740v1_decoy LN:9344 M5:7c7fa5c413301e5c71020ee588c0c1cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001741v1_decoy LN:9188 M5:ec88b5a808b4c20414719ba271b7b641 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001742v1_decoy LN:9100 M5:bad4d9e7865b7ef45b00609846739bda UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001743v1_decoy LN:8771 M5:56e67b8b7db6db977411d3485186a095 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001744v1_decoy LN:8690 M5:f01b9132254ca4074ba5ddc5b74c3d1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001745v1_decoy LN:8566 M5:d96c00c236c6c63764d62b50443c448a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001746v1_decoy LN:8058 M5:14979af859f72ff6efa1b0230cb0bbd9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001747v1_decoy LN:7759 M5:57af8f9f3fb38d3eb268e3d82b21e918 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001748v1_decoy LN:7585 M5:332f1fac3f4964c85f80b068cd91e49f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001749v1_decoy LN:7471 M5:a2a4f2f140a6703cfaa11c1a6ca07380 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001750v1_decoy LN:7461 M5:ec2b8f7282528afa2de49ece1a8d3daa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001751v1_decoy LN:7342 M5:328d2146da22838f30adcf361c5a9165 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001752v1_decoy LN:7223 M5:b0e8a99c193409a71f86d373159d576c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001753v1_decoy LN:7064 M5:3c4648b387bc44aaabddf795d6678ca1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001754v1_decoy LN:6916 M5:c57ae49f10014609feff4fe84c91b31c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001755v1_decoy LN:6897 M5:0bd737852eeaf5c723587cbbd74005d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001756v1_decoy LN:6880 M5:e3d30ef79c4983f0fb99ecf9ac1ca7fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001757v1_decoy LN:6857 M5:cfd001bcac96f459ceac7a135a59cb04 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001758v1_decoy LN:6840 M5:6757a710758275b58b95545211e06526 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001759v1_decoy LN:6728 M5:4d8b4b254f60864202e63aa9dba42086 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001760v1_decoy LN:6688 M5:9e056067d1281622858dfe9830f147f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001761v1_decoy LN:6553 M5:9182e4b1d32ec558a3bd3c94a5254ecc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001762v1_decoy LN:6396 M5:23b91febfc6ba054d109596479f66f8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001763v1_decoy LN:6345 M5:f038e6215610b387550c3b50dddd49a5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001764v1_decoy LN:6295 M5:d0f7f5b63499d6efd4d901a757b1cd7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001765v1_decoy LN:6266 M5:95b3fdc4315475b9c0408bc1f1f49202 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001766v1_decoy LN:6173 M5:4db332a6222e767873f0d9c5051b83f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001767v1_decoy LN:6171 M5:399db4ef3a58b54f82a07da4f0d264af UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001768v1_decoy LN:6120 M5:399aa8c3cb106b5c685092b0de9313fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001769v1_decoy LN:6105 M5:4990624c065870871ce2bfff6bbd26e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001770v1_decoy LN:6099 M5:2e817dafcd97d49c6dfa1eef14a5e32e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001771v1_decoy LN:5893 M5:c8155a905ca8708a397ff91ea578e41f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001772v1_decoy LN:5829 M5:85146edd3b2fab18762262a7455e00c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001773v1_decoy LN:5793 M5:b72258377a1fd9ccb2a374de66066126 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001774v1_decoy LN:5776 M5:51cdb4aa5438f8b50fb21eb9c0959839 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001775v1_decoy LN:5759 M5:f30e892168c0f735365a13e51c8d0af3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001776v1_decoy LN:5716 M5:c03eea2b4a0bc45e1e7df084b89340ef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001777v1_decoy LN:5708 M5:5aba75f216a07e87387db64ea61a7c17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001778v1_decoy LN:5590 M5:09a93eafef7f433e1d2b0d7f3e4c2857 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001779v1_decoy LN:5566 M5:173109c0f5077facb133a124c11fe44f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001780v1_decoy LN:5558 M5:c7f1155230324ea1ff2ec21957290278 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001781v1_decoy LN:5418 M5:8de9a43d5de5e0bd3fbdc3a8ba79bb3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001782v1_decoy LN:5375 M5:2d96ecf69a27a8876ecd20073def54b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001783v1_decoy LN:5300 M5:07550b4953f67bec7ce4f4a04c493553 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001784v1_decoy LN:5255 M5:5c4536fdcf9b0c8f69b6c13e9c535eb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001785v1_decoy LN:5157 M5:af427140b151e2e892fc9a95aec694f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001786v1_decoy LN:5130 M5:254488c203afc62f6965ed57e0644916 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001787v1_decoy LN:4978 M5:a0d7d65d0e93baf75fd6d6d38c774828 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001788v1_decoy LN:4957 M5:6acbb899137257944298b46317165e69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001789v1_decoy LN:4947 M5:cec51f8ad5909dd8e0d3b01e25acbfd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001790v1_decoy LN:4897 M5:a8c98d6ff4dfa416c2dc12f726057ac0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001791v1_decoy LN:4867 M5:8ad543666fcb2e40d2ce2c0bcb95a150 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001792v1_decoy LN:4845 M5:a4cb8d08b6f399bd75e5d3edc40320f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001793v1_decoy LN:4678 M5:02de644cfd20b00dbf3730793f839102 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001794v1_decoy LN:4641 M5:5326e7bd1418a6bd8475fc0cf9383333 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001795v1_decoy LN:4592 M5:bd9741fa5a5eaf10cb939a6822ac9473 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001796v1_decoy LN:4543 M5:465f55fb7df07a219d9cb52c8d369e8a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001797v1_decoy LN:4532 M5:4956e33b08cf6f8939a8053da18b8b4e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001798v1_decoy LN:4503 M5:1fe8c217b537b8ff77877f684728af90 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001799v1_decoy LN:4495 M5:317fc278049d65e36b6b4ac9ea865e2c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001800v1_decoy LN:4444 M5:1815e62f1fb46ebe5a8f20ca027f5e3b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001801v1_decoy LN:4414 M5:7911ec2b81df531fe586719cf18f339f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001802v1_decoy LN:4409 M5:65257f0e3c59604d1b9d1534d2e05cc2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001803v1_decoy LN:4302 M5:dd3519a759d8fb2773d3ce4e05d2e0c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001804v1_decoy LN:4300 M5:1baabb53ab93bc883f297c4fd3313726 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001805v1_decoy LN:4277 M5:a93c3da31dbc626ca07c7a5bb2ae1fce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001806v1_decoy LN:4173 M5:ad4dbde798fb8814acca378a14f36ef4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001807v1_decoy LN:4169 M5:5e7896feb74a66cdf343257968e26128 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001808v1_decoy LN:4136 M5:bb27f7b565373b54a90878f371ecb315 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001809v1_decoy LN:4101 M5:cc0696dc2c3a1cf86aa2bcf3be4fea1c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001810v1_decoy LN:4089 M5:84d4249370d2ab227a405c59d78a49e5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001811v1_decoy LN:4015 M5:13a4dd160518b12fd8900d058ae24627 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001812v1_decoy LN:4000 M5:0d5607a998bac1719a3842db4b2ac324 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001813v1_decoy LN:3973 M5:c54b9de7f485ab7729a4b35a122e5676 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001814v1_decoy LN:3732 M5:0b810e5685d52453ea3c4c3c15f4ab46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001815v1_decoy LN:3709 M5:41dc92336fe91882541594fefae1b397 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001816v1_decoy LN:3686 M5:23342688bf37b8caa294e20464135302 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001817v1_decoy LN:3676 M5:9d214925d90fe222b09cbc3b862d4be5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001818v1_decoy LN:3673 M5:c4bbe25da86730cc4e55c74636296210 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001819v1_decoy LN:3672 M5:3365257914847acb537ffb644b32f6fc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001820v1_decoy LN:3633 M5:9af0a5d44c112d976299f598083354bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001821v1_decoy LN:3633 M5:276e5a73fe80f25706975973fca81151 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001822v1_decoy LN:3613 M5:96044e09be5ba69252a13d71123ac546 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001823v1_decoy LN:3605 M5:2402d86c44cceab83a438a4af0e24939 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001824v1_decoy LN:3592 M5:8e4cc1d20a97fbddc8d430158ab2f8a9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001825v1_decoy LN:3586 M5:b0cc5444bffc7aec1c01a855b52ec3a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001826v1_decoy LN:3584 M5:9b94b86d924e51adbe1fa241e9eb4980 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001827v1_decoy LN:3577 M5:2ed768998d7e36315f9b21917eff299e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001828v1_decoy LN:3537 M5:b9fb044a6692c6bc7dfdca8fcb811f79 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001829v1_decoy LN:3510 M5:27176699c6daad8aa9991c140bb4dceb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001830v1_decoy LN:3509 M5:dab9d17bab4efaf2e54273afe4017807 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001831v1_decoy LN:3488 M5:cc824ab2de05f959425033f7d1b34c54 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001832v1_decoy LN:3473 M5:05d718c7b92a89c2a4b961ee6c0b008c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001833v1_decoy LN:3445 M5:c374915181a1f43da7688151fad2c609 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001834v1_decoy LN:3427 M5:fea3edb105a855523da01bbac633b515 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001835v1_decoy LN:3395 M5:9323a036098595da4ac07b2de10ef332 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001836v1_decoy LN:3367 M5:19070dba7b35a5d94fffb7a4c9bc41d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001837v1_decoy LN:3337 M5:21d6bebf3b38b8e623684b9e0e707909 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001838v1_decoy LN:3324 M5:445cf21cdd79290b7e69a748db3691c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001839v1_decoy LN:3315 M5:f1f38a814378ad3f53ab744d03c6faec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001840v1_decoy LN:3313 M5:943531134a853079b30be3d92c9a37ee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001841v1_decoy LN:3283 M5:aa33f6f89273b5d970d09f46c4acffc4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001842v1_decoy LN:3250 M5:6c7e5a0af5d65078902e7369e77c3eca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001843v1_decoy LN:3247 M5:a40dde4dacd9b3af8d1346af020f3826 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001844v1_decoy LN:3237 M5:19c321a618697df2f90bf2623e39e09a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001845v1_decoy LN:3235 M5:858d4ef2c5182eec9a3af185040447c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001846v1_decoy LN:3200 M5:5198826fed1230b23329839aa0fe293b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001847v1_decoy LN:3195 M5:bc41aa22979bdd3975774235ac080c4c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001848v1_decoy LN:3175 M5:e3cf116e22c63b9ca8f5f83ef00c9227 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001849v1_decoy LN:3158 M5:2c546de8c11c8b020271c0a602c8e914 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001850v1_decoy LN:3143 M5:b114278cb76d8a47cd1ea131b6b01c46 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001851v1_decoy LN:3139 M5:836cf9f277e7abe51f821eea4e72f9da UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001852v1_decoy LN:3138 M5:99ec6bed7ebdeb700737347dbb987feb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001853v1_decoy LN:3136 M5:3ce9684d466895b5b1ebc7b730c1a22d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001854v1_decoy LN:3132 M5:4133dd5a44f06f0676fd2c8f8f2e532a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001855v1_decoy LN:3132 M5:1d73546845d822a91cbc6d9f4e78688c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001856v1_decoy LN:3095 M5:318534fba07d7da9181de4e227a5ddf1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001857v1_decoy LN:3094 M5:bb93d34eb9f7a80b575056a9785171f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001858v1_decoy LN:3093 M5:b4c67202f6c2cd5231bdf62ec60bf138 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001859v1_decoy LN:3059 M5:80bd25131b27cbfb7222fb139cdc03f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001860v1_decoy LN:2985 M5:3b138424ffa05470fbaf4e81e0b11722 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001861v1_decoy LN:2975 M5:128ceb0f1b4c41d3107868af22cbd590 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001862v1_decoy LN:2967 M5:f0bd303b1446335f781e0c2f74d28de3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001863v1_decoy LN:2961 M5:562fe7159c1f06d7750af9ef9c3d3e6f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001864v1_decoy LN:2955 M5:8028d9a1447473820a8c0b0e88325ffb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001865v1_decoy LN:2935 M5:2fb53f3d6f706d8f5738f63429c8de36 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001866v1_decoy LN:2933 M5:a22cdeb6c078d85e19e089f7f9efb045 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001867v1_decoy LN:2909 M5:1fd7abd65c5be9c9dc924daa280f43be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001868v1_decoy LN:2904 M5:5e863959002b0c580d4820a7cebdbf48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001869v1_decoy LN:2892 M5:662acc47cf615cef44478309a902b41b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001870v1_decoy LN:2886 M5:b83662f3f83e28d70a9133675fbe1b1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001871v1_decoy LN:2885 M5:0734ed3f7e1aed9828f22b347664e98e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001872v1_decoy LN:2878 M5:2246626d2530d2260b223f28a96a624a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001873v1_decoy LN:2875 M5:a306eed4bf0048509612220ddfca4dca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001874v1_decoy LN:2861 M5:45f856116b6642c701e0671967fb8bfe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001875v1_decoy LN:2856 M5:a35e380dc9bcacbbc7a29692f039feb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001876v1_decoy LN:2838 M5:e010e81c2ae154a12105872faac2d033 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001877v1_decoy LN:2801 M5:c830abeee2c0527ca00ee4004e2f26f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001878v1_decoy LN:2797 M5:9f0b46d448ffe81f55a61311b72408d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001879v1_decoy LN:2788 M5:ff37de9e839a483b85b9978f165e2aa4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001880v1_decoy LN:2773 M5:45df0f07d5235217631f54d4decf8ce9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001881v1_decoy LN:2755 M5:22fbad6b463ff553fbe7482783018f9b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001882v1_decoy LN:2754 M5:f5751d68ef59013dd8a924405b61c766 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001883v1_decoy LN:2743 M5:68564eee42e550492f0bf366f22345f1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001884v1_decoy LN:2725 M5:c4477959c12bb115614ea504cf712579 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001885v1_decoy LN:2722 M5:d221c4dd4b764a3cf767ae2f408cd855 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001886v1_decoy LN:2682 M5:6a91b1b63b686c713c6daaa9a5b28c48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001887v1_decoy LN:2669 M5:176039ee6f1d1ae8eaf240056ad7e1ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001888v1_decoy LN:2663 M5:ac2fb1176f2733ad7fcdd03cc15e919d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001889v1_decoy LN:2652 M5:f14b36f702c6db220606dd6696067369 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001890v1_decoy LN:2647 M5:82b5cfe2b5ccc5d14445809aefc4acee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001891v1_decoy LN:2635 M5:fa2959b22afb179b2e6b195f5bba68bc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001892v1_decoy LN:2633 M5:62d8ae1cef408d715d22ae3ba3dfb9c6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001893v1_decoy LN:2629 M5:cb667e2bdb9486da5a227dbd50803c45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001894v1_decoy LN:2612 M5:923dfb32d8271c70d8e8cd9288621481 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001895v1_decoy LN:2599 M5:691f294fc31908fd910ca9dac0cd4362 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001896v1_decoy LN:2566 M5:9cce036b1dc05a82d7a2bd677688b7a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001897v1_decoy LN:2556 M5:975e1058290d80f4ab2cf207313135aa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001898v1_decoy LN:2551 M5:e52be60ead1084c5dbd6851964b3d575 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001899v1_decoy LN:2551 M5:71028cef766fc1dc75684dc127ec3f73 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001900v1_decoy LN:2538 M5:e180467b2632a688c6d3b1e58b17892d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001901v1_decoy LN:2538 M5:bcaea2754972af50dff62993504801c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001902v1_decoy LN:2525 M5:647f59f3a4ad408b803a4c971db5651a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001903v1_decoy LN:2498 M5:80be471faeb22fb67458b7bb8e00ea3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001904v1_decoy LN:2496 M5:be7e3722d3c1a45b98f79cf89cfc235f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001905v1_decoy LN:2483 M5:609856bcc6aeb799c0a57773bccc7598 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001906v1_decoy LN:2475 M5:e64a6fb38d225decae7e079ea7b9732b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001907v1_decoy LN:2469 M5:e2b450a085ac14eec02539b8c1bd6bed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001908v1_decoy LN:2455 M5:0b0b3b8bce8db6dc091b3429802919bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001909v1_decoy LN:2444 M5:6a091545fa77d96dc253bedeaccb6692 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001910v1_decoy LN:2437 M5:ad77fbca3fa49f1c970e8c64b58391d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001911v1_decoy LN:2435 M5:233e3f635c3bd0fae9ed04c155c799a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001912v1_decoy LN:2427 M5:e22283fea810c2cc4c92cc5ac5ead914 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001913v1_decoy LN:2419 M5:ea7d2ed4522c0ecea6389a3b9fb7bcfd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001914v1_decoy LN:2413 M5:953de10ba34733832f5bbcb37e12c5fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001915v1_decoy LN:2412 M5:ea8ef9ae0476686a6cbd488a0202b2bd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001916v1_decoy LN:2400 M5:0198ad2dfad702357fb849a43500deaa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001917v1_decoy LN:2399 M5:d566d3ebbd937aa7ba415298188a1c93 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001918v1_decoy LN:2396 M5:536fac7c509791bd524e9da425d7fd93 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001919v1_decoy LN:2393 M5:522e11044a40018a2f755d9d4fd8103b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001920v1_decoy LN:2386 M5:aa9e69c6d5f6d04b4030be6b3226964c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001921v1_decoy LN:2384 M5:b4d05aabf35963906d1861ef966a4709 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001922v1_decoy LN:2382 M5:97749df462ddf825702a260f9aa2f021 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001923v1_decoy LN:2382 M5:3c0d3376e16a20d57497a815d2475873 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001924v1_decoy LN:2367 M5:e76216aa8c58c6949a9c04cfa9b95995 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001925v1_decoy LN:2366 M5:4b4ae46d37a4f45b63c4b8c3cb63294c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001926v1_decoy LN:2362 M5:589965b553dd53d631ae9970b0c65403 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001927v1_decoy LN:2361 M5:aaac326f62cef7cc31b28e60534d0a3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001928v1_decoy LN:2353 M5:2c60f763bb39af3328df40c97b8e4f0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001929v1_decoy LN:2349 M5:5e3aee054d3451ec9e4e18ee277b5efb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001930v1_decoy LN:2348 M5:c885bcc19373dca31580a65ec6fc7e48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001931v1_decoy LN:2340 M5:b36f2e5330d409cd8ba0973000358c52 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001932v1_decoy LN:2339 M5:63104b56d2278c3cb82c3d6f23d8977c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001933v1_decoy LN:2336 M5:2c7518f9e4ed0d994be4698762511cf3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001934v1_decoy LN:2333 M5:308cac07c79336f8162a8c2e855f7762 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001935v1_decoy LN:2330 M5:bb4702fc9e6227387e8ce3498109d998 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001936v1_decoy LN:2327 M5:ddad50424c1114dd98bc0202ac1cd9fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001937v1_decoy LN:2318 M5:28804e3d50bf7764ac6b1897b1128790 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001938v1_decoy LN:2293 M5:2aa344c0d737b4d9042325fb5a858bee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001939v1_decoy LN:2292 M5:22b000d9737a411cb3844951ebc17b87 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001940v1_decoy LN:2287 M5:44ec350c3d506da4df5a5c6c60683796 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001941v1_decoy LN:2274 M5:c2d8c7fed619477a490f674da302b98c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001942v1_decoy LN:2274 M5:bae016f221de9bcae9788e26dee71d3a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001943v1_decoy LN:2267 M5:88696dc559363af860e8828eed45eb07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001944v1_decoy LN:2260 M5:be18f9c08c02b23d6bc3e38f71843b69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001945v1_decoy LN:2257 M5:bc65d96f85bac2cf76e5db2847c7c723 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001946v1_decoy LN:2240 M5:98c67c23fdf01dd3a05e1213470d56d5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001947v1_decoy LN:2239 M5:0ec5d58555d46a257ce56425c730f4d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001948v1_decoy LN:2232 M5:b8901c5291103859cc240e5d6c96c4ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001949v1_decoy LN:2230 M5:976e267cf6d42bc3c67a345bb56e707f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001950v1_decoy LN:2230 M5:f179f9db12db687102c9a63bacaf36ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001951v1_decoy LN:2222 M5:f5a5058d6f869ae64c9a4b69439d4114 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001952v1_decoy LN:2216 M5:63f1d3d20deaabfeeb68d11879debd0d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001953v1_decoy LN:2214 M5:b09d559a9d44f7eb887d75fd192524ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001954v1_decoy LN:2210 M5:7d0a884bf7fd09ebc9175286c058635f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001955v1_decoy LN:2203 M5:6005c464e19405b659d2d2e09778b06b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001956v1_decoy LN:2197 M5:a15768dc613354cb73ec09a4c5c814ee UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001957v1_decoy LN:2196 M5:cd06502f93b8e17fd05511c84cf6c523 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001958v1_decoy LN:2196 M5:c315413cc7a46460c67bde0cd8e78b71 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001959v1_decoy LN:2179 M5:708d5a6db13c3ac37d5e90e0cc2a8c32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001960v1_decoy LN:2178 M5:e217486e375bc7a5925ec6cc7fa9e003 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001961v1_decoy LN:2178 M5:dd793d59199c7f60817d549e0c149b15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001962v1_decoy LN:2172 M5:aff6ab00ffade197cf1c12f043e2cbe5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001963v1_decoy LN:2170 M5:2cf0f6c81889758a8dadc195f7928b09 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001964v1_decoy LN:2167 M5:c20e5ca2be0e106f7d0694f9e67a3ea2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001965v1_decoy LN:2167 M5:df1f17762d17818e216feee97299c37b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001966v1_decoy LN:2157 M5:071891675e2ba4476534e0035ef13cef UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001967v1_decoy LN:2153 M5:59ad2443710d81ac651620a8f216e7c0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001968v1_decoy LN:2151 M5:2c5f2ad4a6812000584bf131c918c3f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001969v1_decoy LN:2147 M5:e6458e552c51be648c8cc8ca7c01ac98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001970v1_decoy LN:2145 M5:408a6d5ea59519609696b4228f0cbf97 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001971v1_decoy LN:2142 M5:90b747485a6b12433a92fb55202869fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001972v1_decoy LN:2142 M5:7de080d8181d8066e63905fc22a7ee14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001973v1_decoy LN:2136 M5:b611d1ca78ef211201bfc0b6a68e74df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001974v1_decoy LN:2130 M5:5f1f983972aaf38be9f2fd9fbcef8a9a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001975v1_decoy LN:2128 M5:0323f4f5194c96dc1bcb919f06960a20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001976v1_decoy LN:2126 M5:c142de1e2c1924e545f116a373abafc1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001977v1_decoy LN:2126 M5:6ec3293375949e50d082d3f5639ba434 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001978v1_decoy LN:2119 M5:e1eeff28afd215be837a02bfe59d8294 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001979v1_decoy LN:2107 M5:eee07feaf8a44d69317e6b46e8eee089 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001980v1_decoy LN:2091 M5:ea5e9165f2f579567e1addc7de1a7a6c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001981v1_decoy LN:2087 M5:635626092c6f01e77135d2798172beb8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001982v1_decoy LN:2086 M5:269d34f32d02129323bbc21d6336cc6f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001983v1_decoy LN:2083 M5:5aadd7636d45bda287185de4cefac8d9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001984v1_decoy LN:2075 M5:ae2d128f1ba2ba34942dffebf4b7905f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001985v1_decoy LN:2075 M5:0f195e7dde4153887f4f0615cbd730e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001986v1_decoy LN:2072 M5:b236a0d4a67c91d699b3cdf5adc47a71 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001987v1_decoy LN:2068 M5:2e34944b2779f309285d9db7a20b69ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001988v1_decoy LN:2067 M5:233b964d4a2e5bab26b7b996dc46d4b8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001989v1_decoy LN:2055 M5:795ffd63a0e2d97ac00ca57cdab045eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001990v1_decoy LN:2051 M5:5e69880a7e6f7bf744cf4b305624a7e7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001991v1_decoy LN:2050 M5:abba4241716dcac69b95e887f8358859 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001992v1_decoy LN:2033 M5:096ca0754d9f8f4cf7a582f57c5fc083 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001993v1_decoy LN:2024 M5:288495a36e0f4b1fc8e90cff6d4734eb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001994v1_decoy LN:2016 M5:96d95a271cf5d103433921842ead9915 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001995v1_decoy LN:2011 M5:cb8560d292475a0fd481b0e794ed0186 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001996v1_decoy LN:2009 M5:a6503ea36c1b69162d3eda87c4f33922 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001997v1_decoy LN:2003 M5:d3a1bed41244634725882235662d11a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:chrUn_JTFH01001998v1_decoy LN:2001 M5:35916d4135a2a9db7bc0749955d7161a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:01:01:01 LN:3503 M5:01cd0df602495b044b2c214d69a60aa2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:01:01:02N LN:3291 M5:743d9f66c77fc21b964a681e0c6de2ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:01:38L LN:3374 M5:dd27b7fe617e92bb77eea00fede6fd15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:02 LN:3374 M5:3ba47a11a8a5b47ccb855308e26a2f4a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:03 LN:3503 M5:554d43de8f2a97cae068169fe3d8462e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:04N LN:3136 M5:072ea3e53c79f3d00e1f1a7b492b0a8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:09 LN:3105 M5:68176666a98582ea361a9181d69679af UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:11N LN:3374 M5:b9ad3338cc73e2a99888a36e04c29f75 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:14 LN:3095 M5:0385be87eb49df4c59d7487495e3b1b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:16N LN:2985 M5:10150ad21301a29f92e1521530fdd3f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*01:20 LN:3105 M5:05dc0384da2f751afe549a9bfdbc3037 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:01:01:01 LN:3517 M5:174a8ac24d2e6625c1e7589219d6fb84 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:01:01:02L LN:3287 M5:96a6b1f55c41a7da24857ab8844a7a22 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:01:01:03 LN:3023 M5:0ac19a55ed1a22389a2d667c42ba1218 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:01:01:04 LN:3516 M5:f6a0bed71d059f8387d9d22e2b0c46b2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:02:01 LN:2917 M5:a99f213073198aa5a532f950135f6d1d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:03:01 LN:3517 M5:8a795bfd81ee3440e8d0fa3e5d09cd35 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:03:03 LN:3148 M5:6ec792b8a5944a6edb0c1acc18ee1418 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:05:01 LN:3517 M5:dbb2cf4bd26ccd1d7c17327d2cbc119f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:06:01 LN:3517 M5:00a663d257ac9d4d6eabae95f6fb6d8d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:07:01 LN:3517 M5:2833baebca84548dd4e37d79642db779 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:10 LN:3517 M5:61bf8d00643229eaec4eb8def24f1a98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:251 LN:3517 M5:d39aa010841e51a5fc857e82a3378d96 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:259 LN:2978 M5:fcedee868a44319f80c453cf7b833abc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:264 LN:3002 M5:6cc807b0948af0b655ad0265629b7906 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:265 LN:3148 M5:5e00ac7ffc401cd5f866e17cd6b7d76b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:266 LN:3084 M5:fe25829d3992130e1ca423e98507b843 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:269 LN:3101 M5:724d76a112c0b557ae52e35dcc2973dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:279 LN:3103 M5:0aef01822ccf2bada1e9a2ce8c771711 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:32N LN:3517 M5:02ae1b32f6268509dbfab828c8339a36 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:376 LN:3104 M5:79dba7660c7fa948d23f2703e80eab7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:43N LN:3218 M5:68aa8847e8ec55dce252be3812b03bac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:455 LN:3118 M5:6903220f41d0f8754d6cc3ff6fb3fbba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:48 LN:3517 M5:2947ea9bc4c28b64abba44002460a38f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:51 LN:3109 M5:84c0d488e0a5f95cb3587efd6c84b00c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:533 LN:3217 M5:34bc003a99760f4ec928572b3a747094 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:53N LN:3305 M5:108696b83e605fa59b435cfb8406edf4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:57 LN:3054 M5:bc22083129551a34dbbdfd07e36f763b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:60:01 LN:3112 M5:b58aed6de00585e7f185bf3eb573cb45 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:65 LN:3387 M5:03702f960713a7c42469b033b7ef05ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:68 LN:3109 M5:74777150832f351162301c7d04a64d48 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:77 LN:3371 M5:9e5ec638d0de688fc20aaf00b74b5a9c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:81 LN:3309 M5:2cbf6e9f368ea4387632204bbdcba76f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:89 LN:3371 M5:782c923ac311a590d29e9e85b6a6b5e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*02:95 LN:3388 M5:36b421cc1baeb1cc37291f98566cc5f9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:01:01:01 LN:3502 M5:bc204fec41a0219e8044aae1c5a3a6ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:01:01:02N LN:3373 M5:c9d431f34db1cf123c6dec89ae7075f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:01:01:03 LN:3094 M5:4744e9f0617b3715ed23779866c2e1ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:02:01 LN:3502 M5:d54cdfb5d7a25804bc3b5a3711d1e42c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:11N LN:3404 M5:fb87c0184303005cf961e7e6bc65a3f5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:21N LN:3095 M5:665152840dba04b1bdbfefaf3d4913ac UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*03:36N LN:3142 M5:c5889d985a6d5ac0f86ee9a5f1449762 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:01:01 LN:3503 M5:1b32d51d3415a99c68971d21b686744a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:01:18 LN:3503 M5:3283d7a24c7ad61f9024a8f7d76d5bd6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:02:01 LN:3503 M5:fd13f748ca0c574e318250f8dc0f147e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:05 LN:3373 M5:78f70065b11cbfb3f502af9802ae3b7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:110 LN:2903 M5:ca7ce57dc961032a37af87fc26cb626e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:25 LN:3073 M5:e1ba0819b77d173c0a9c6697f76f3e50 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:50Q LN:3362 M5:ec1d5f53ad6427e623c41a11393bc518 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:60 LN:3241 M5:6815ddd990a7231bc8dc40a414cba309 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:69N LN:3500 M5:dd8ce51ba7bb514b56ffcb5d7566dcae UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:74 LN:3227 M5:057f5c85ad1f09e82b0f04bbc54f9283 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:75 LN:3184 M5:9975bcb416ee87f54025f71692bbc1ce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*11:77 LN:3233 M5:615353e3ceac5a04cb381339a800ad9a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*23:01:01 LN:3502 M5:edf8a55a69faad1aab25516e1126b0c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*23:09 LN:3104 M5:fbcac94109d55d95b94b869fd6e42705 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*23:38N LN:3020 M5:83381147ae6889a0af532bcb7af83e6a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:02:01:01 LN:3502 M5:95495e041aa53313d12032c49baa1b1e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:02:01:02L LN:3502 M5:d91ba7d36f6472faec4c54d1f476c9f8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:02:01:03 LN:3075 M5:a89ed5d11db1d882965e052a6a049217 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:02:03Q LN:3247 M5:d55685eded22f07791df08b502ad3872 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:02:10 LN:3356 M5:739b4ee3c4a555c2b699fa358ad07744 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:03:01 LN:3502 M5:d866111fbc6e2ea2cf086915b28442b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:07:01 LN:3502 M5:872c7be9ec525309cd91d949580a87e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:08 LN:3502 M5:f0bbd52535d5ef86e21c4486e1761207 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:09N LN:3502 M5:9d122834f5117a876ff9ef768a61672e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:10:01 LN:3502 M5:035534e6b4f7a7b2b20506a41290b8de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:11N LN:3503 M5:bb7085dbbd00c565896a17505dbd06c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:152 LN:3176 M5:6d81297ebe64b08f9ec9e8adedcde902 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:20 LN:3502 M5:eb4b55e730512095c889a1c36a7fb51d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:215 LN:3116 M5:f6c6ec83d1c70ef4bdc483abf45a141c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:61 LN:3043 M5:8ae770b761bfdf718a8cf382b54fc71a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*24:86N LN:3415 M5:0e1108b26db8b22f177b460c8a34c017 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*25:01:01 LN:2917 M5:2e22cd0725822e29d3f0df6844339714 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*26:01:01 LN:3517 M5:51cabb4c3149d169568609c1906d969a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*26:11N LN:3091 M5:112419eeaa846213785eadd7380308a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*26:15 LN:3217 M5:2a2c8f5ba64eb0110f2de0d8b74a5d98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*26:50 LN:3141 M5:9a1a6cdedaef2a3c66ca98f77622fe50 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*29:01:01:01 LN:3518 M5:6a6e4c826d8743d5f327d8906a311270 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*29:01:01:02N LN:3303 M5:497a11cff2fde28a5c500d529581c57b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*29:02:01:01 LN:3518 M5:77d1ca0d5ea97ccf052d85d9515117ed UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*29:02:01:02 LN:3518 M5:53bad80334b21427430686d44dbda04d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*29:46 LN:3310 M5:b4635ec707263c634b27769afb46323a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*30:01:01 LN:3503 M5:c6b537ed485b72a6b78cf527493d8ca4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*30:02:01:01 LN:2903 M5:65d618ee20ba439ef5ae408a182e25df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*30:02:01:02 LN:3374 M5:4031b0ca00a39d4d076dcdc2808f590d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*30:04:01 LN:3503 M5:21f4d62eb6e85f6257661e4c94b68aa4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*30:89 LN:2903 M5:694bc9c1c804f437646697ba9d968bc5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*31:01:02 LN:3518 M5:6e24e36ceae5a9c0dfa1cbe4520b4425 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*31:01:23 LN:2918 M5:61060b6e397e85fd905668b5876d8c50 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*31:04 LN:2918 M5:2d4a59604c2d3d795f3647a186685ad9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*31:14N LN:3090 M5:afc943d7553ef55e21a855f5e266c8a8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*31:46 LN:3075 M5:ae87be36c211dbf040022ade7713d2c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*32:01:01 LN:3518 M5:d3a7f1fb642659538a431ee2c1d8113e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*32:06 LN:3389 M5:06c6aff700dd7ddd935fd83f2def16e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*33:01:01 LN:3518 M5:f1f4a4e1fd2e774e4003c4f94c67b36a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*33:03:01 LN:3518 M5:4e7abd41072e8f3c62c49c683497d39c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*33:07 LN:3389 M5:4ed7b17c57b32199660f285b9e53bdd8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*34:01:01 LN:3517 M5:c79f20a6bd41e2e41b6e067222b4af42 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*34:02:01 LN:3096 M5:a3a26d9b6292599851c207c193d92b23 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*36:01 LN:2903 M5:a923a3fb762fa22c7956888b6634dbcf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*43:01 LN:3388 M5:a5e6a04ac5537f379e03956d57cefe3d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*66:01:01 LN:3517 M5:787d6cacbda41669a942dc4b54c4f7e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*66:17 LN:3075 M5:5f0734606934302fccb508a2f5d3b915 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:01:01:01 LN:2930 M5:93bb5b64ccd0bab2bc0478d6bd0e8854 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:01:01:02 LN:3517 M5:957d09385dbaaadcf87f85227a2a43d2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:01:02:01 LN:3517 M5:0e43acd52311b6485d4542edf7c6d286 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:01:02:02 LN:3388 M5:fbfbf3067287661249f797a0701cbb58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:02:01:01 LN:3517 M5:21b71bd572d6dda388a4d60211edba0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:02:01:02 LN:3506 M5:00fd36e8b22143b0adfbe025e2d5d2b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:02:01:03 LN:2909 M5:df913a8914cc697da83538a7b8e1898e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:02:02 LN:2916 M5:a34e21e7b4de5c05f67281bf68bdeb66 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:03:01 LN:2917 M5:9eaaf636f6b7bcb8557a2b843eda3b60 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:08:01 LN:3120 M5:c2e72d05d212b232f180c5020ad652ba UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:113 LN:3070 M5:474b432629b8d4cc0486021e71e3dabd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:17 LN:3134 M5:f44b78e8df335a94b8ba2ee62214ed82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:18N LN:3237 M5:250389f8d56a86c0037d9e10ce88aad4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:22 LN:3119 M5:5a8b2633f1fb32860f4d5df30e8677ca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*68:71 LN:3198 M5:dd6320e01a52a1aaa9224cd4c39807e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*69:01 LN:2917 M5:02bd84b3d7b9d16ec8546efc41b4d0c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*74:01 LN:2918 M5:948aa5c51d96d4ecbe3fc862f0214a3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*74:02:01:01 LN:2918 M5:4b972360085a8e764c52f0a30ff47673 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*74:02:01:02 LN:3518 M5:97a7f1d63575aaadaff78b53c7bdd231 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*80:01:01:01 LN:3263 M5:5405ef04524037ea84ce1fc83dc9ac20 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-A*80:01:01:02 LN:3055 M5:5cc77fd13bd6123b7300d6da19ae5c13 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:02:01 LN:3323 M5:5eba87e3d51a0b86a498f97022e5714a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:05:01 LN:2676 M5:d41a7c05ac284e89b86b658d33564b3d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:06 LN:2676 M5:a5d7749f86004926ffe9fc104b1e99c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:156 LN:2967 M5:8cc8227691836b87823ff2228f9b25a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:33:01 LN:3239 M5:6ab184ee1fa9167f8857a4d6a7b33da6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:41 LN:3266 M5:9b52773dd0c042b641ea254944e932d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:44 LN:3270 M5:03ee83997251cdeeec6269f547238122 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*07:50 LN:3323 M5:e369192a36b773e819b37507a823343d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:01:01 LN:3322 M5:4ed3fc74f9934e922cc8345b00459784 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:08N LN:3035 M5:3d8eb010a8cb9c3da8d4eb759dee63f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:132 LN:2675 M5:a863b21bd08f28404b8b991486beec56 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:134 LN:2959 M5:cd3c81892d9127631465bd4454169e3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:19N LN:3322 M5:c64401d837f2f4eb51a6f89375d02401 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:20 LN:3322 M5:87e42f5c7d6c6b2f1990fb2477271f14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:33 LN:3322 M5:704bdddcfc1c52e4181d4c12a4e25e54 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*08:79 LN:2676 M5:f08c9b4724eaaedae63d87b79af851de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:01:01 LN:3324 M5:6a8fa9ecf6d7b7a57c77b21c908740b0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:02:01 LN:3324 M5:120d59fa894b11fe23dce75e3f4869f2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:02:03 LN:3323 M5:d52345523e7897e545fffd4718dcccf4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:02:09 LN:2919 M5:ef118f2b15e542e80c1b9c162ad69345 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:08 LN:3324 M5:11f30cc59a8a4b6d4b43144c294440c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:15 LN:3323 M5:0684fd43ee5428bc529561b33db2f2d0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*13:25 LN:2689 M5:dbbc5af54a1289ade5eb273e2f7aee7e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*14:01:01 LN:3312 M5:3764a48b755f8e31055b0e861e4c74e3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*14:02:01 LN:3312 M5:26b567e4c01ece594ca1d0f0b18ae0c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*14:07N LN:3255 M5:74dd725f56e8dad2652028bd0b496125 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:01:01:01 LN:3336 M5:b00240fd0143d1f67ee96b53a30adcfa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:01:01:02N LN:1208 M5:1c5acd964349055939ac8654093dde0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:01:01:03 LN:3026 M5:dcd416dc1f3d5d91d7374096d09a68ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:02:01 LN:3335 M5:25973b11c58535f98da0dae1d36e1ce7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:03:01 LN:2689 M5:bc488eabb3f7519b04b6a795acdf81c8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:04:01 LN:3052 M5:6ccb4f7a3eeb9e8ed44236b5092c2bd9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:07:01 LN:3336 M5:7630abd7e06b6d17b3542a0efdb6c780 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:108 LN:3283 M5:80b9c94caa804a2bd7f2447b9d24cf82 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:10:01 LN:2689 M5:c0699fd2bce33c66fbad3066177f17db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:11:01 LN:3336 M5:c3971913c6e6b41e2f1bbb56026c60b0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:13:01 LN:2688 M5:a5d9251e2d32c20001b7845134141a39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:16:01 LN:2688 M5:fc7d48526274847fbb255137159d9d47 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:17:01:01 LN:3051 M5:fbfa24d81ff319e1c9a77eb23a5c5af6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:17:01:02 LN:3051 M5:547e0689ef02b1b497deaa785f374c32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:18:01 LN:3336 M5:79c954404a5e1b10f46264fbb3c6b32a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:220 LN:2878 M5:a87213c0fe840a0cac742fc92c4a203c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:25:01 LN:3335 M5:316c1666b4ee7ffb47f4922157d121c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:27:01 LN:2689 M5:e01a2e586f79a078cc425bfca6367182 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:32:01 LN:3336 M5:4411f20a66f0e08032144115799b7530 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:42 LN:3333 M5:e6b197d6c9397d8de0ea8de14a814b05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:58 LN:3336 M5:1c0d71c98a79449eabd99e163692bd6c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:66 LN:2902 M5:a5186448b35a7b3923ccdae5bdf4bd8c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:77 LN:3336 M5:a4984835f8285fd5518e523394749864 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*15:83 LN:3337 M5:322d07dc63950f70a35cd4a6cb9ba2ab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:01:01:01 LN:3323 M5:b0017c6a0823ba043caa28c260a41b12 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:01:01:02 LN:3323 M5:a51373cbff28c2af5ebec8fac8e33cca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:02 LN:2686 M5:ba01be9495db45e331f731023378e599 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:03 LN:3323 M5:207b298dc49c700b8a16865a72eb7c7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:17N LN:2979 M5:01b001b48d8d89f7671231f8cd45ca05 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:26 LN:3323 M5:655b4f8cf9b575997c39e45df3664c8e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*18:94N LN:2970 M5:67c613fbd0339031dc79fc91e294348a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:04:01 LN:3325 M5:4380eeb25545f28b8d6a8bf602e7c2f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:05:02 LN:3325 M5:f60437ab7a86bf431ed0854fd2641cdf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:05:18 LN:3321 M5:ff2e5f34be64b46f1e15d1091ea54645 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:06 LN:3325 M5:4c3f65501973406722a9c66c6f129061 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:07:01 LN:2677 M5:61c1b2092f60d8b21a413b8e6846c58d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:131 LN:3325 M5:08dcdc60c6c29f52d100af9900b30e39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:24 LN:2677 M5:c0e3b858ba702a5959d35e51b8b75406 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:25 LN:2677 M5:f1a1a745dc082e0c0a91813d3f12e52d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*27:32 LN:3325 M5:ef9b650861b7081940788ef1618a074c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:01:01:01 LN:3327 M5:72eabdc1286e5f8faec021e4c3fbd212 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:01:01:02 LN:3327 M5:8d6dd73094fd1458c95866fab0bccfb3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:01:22 LN:2806 M5:198a61b3d4b83cfed1fa018f0b5313e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:02:01 LN:3327 M5:69e50f2988b92d745c93ebc9877eace7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:03:01 LN:2689 M5:866f05a4324734d3cca067f98354488e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:05:01 LN:2690 M5:c6cc89ecf3130ee79cefb981dd362f0c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:08:01 LN:2689 M5:6baeeca7110354530934af4af0cccd8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:14:02 LN:3327 M5:99db27c20c358808c9e75b090eb54d06 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:241 LN:3042 M5:9fd1849b96ef49c7b705acfdfb430e8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*35:41 LN:3327 M5:8526562f5800fb5180e418bdf4b6e56b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*37:01:01 LN:3324 M5:8e4396d6b7c04761713fc800437312bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*37:01:05 LN:2687 M5:c4312bfb5ca6f84d1c4b2c444107b844 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*38:01:01 LN:3312 M5:205bcc2884f405305b551c58c25d7232 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*38:02:01 LN:3312 M5:501fd25edfcd87eeda45de0caa78e1de UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*38:14 LN:2738 M5:7639a6e735e55ac574403a7970e113b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:01:01 LN:3155 M5:eee292da1356cc9c3bf1b333e89661d6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:01:02L LN:3153 M5:cd343572a20faec9ea375660b4b08feb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:01:03 LN:3312 M5:e3fff974f234f099f66e0fe53a93ef2c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:03 LN:3155 M5:2e520e3148dcdf7d8fc7763872d61957 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:16 LN:3155 M5:cb333cf3f06cf66b86f11654c941690f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:01:21 LN:3312 M5:6e1fb83beab576ba8c39ff0237d40277 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:05:01 LN:2675 M5:bf2d7c62a16bf00c2559565daeb0538d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:06:02 LN:2674 M5:77758f231bef5ff95e5a4be58d38dde1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:10:01 LN:3027 M5:fb13d6eeb084ecb4d241e45485ef36e8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:13:02 LN:3255 M5:5836ea0fc7935bd208b6caa597934b98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:14 LN:2765 M5:76c31f2b103685de65a25008bf484f11 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:34 LN:3254 M5:840ee3b914f83a21f98cde71f89d77d6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*39:38Q LN:2675 M5:55571273ba2d1a50d1f87b47ae59d9aa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:01:01 LN:2676 M5:afc20b1efd5b1b479db2e62fb8b88337 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:01:02 LN:3323 M5:e80885c2e036b557e2bd1ca6c2b6667f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:02:01 LN:3258 M5:e6e0e85a0fab0b1e17686742e0ace0e4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:03 LN:2677 M5:a4f89215e2d83f162c338ac822bfb46e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:06:01:01 LN:3325 M5:9a238e4bba71b906acf8c46254a9bc31 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:06:01:02 LN:3299 M5:ee4804e6a27f57a5c37e372dc6daa312 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:10:01 LN:3304 M5:5d4db1b120369dcee6c52764f69bfb3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:150 LN:2800 M5:f889007857633aee1ffcdf1fc924ca96 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:40 LN:2677 M5:549008522cb77878ea5c2cf5bd048064 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:72:01 LN:3283 M5:7d61179ccd5e3f1fe4b4976456960d02 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*40:79 LN:3257 M5:521e3a50ed37f445b431b465b0d4ed77 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*41:01:01 LN:3322 M5:17390ad33a3efae3617d3a175671902c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*41:02:01 LN:3322 M5:c886279897cd7e75a357ac3218a1be5f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*42:01:01 LN:3322 M5:154e1df41ca09b47ebd7e365545f6e6d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*42:02 LN:2675 M5:59d27bea1d07ea5695101e714f65812c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*42:08 LN:3165 M5:d860761be3a99c6724146e606a28aac9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:02:01:01 LN:3323 M5:477ac3a92e88828aa579f38ac0fc39d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:02:01:02S LN:3152 M5:05fc8de1a02c971099db853c790d3fca UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:02:01:03 LN:3152 M5:2202501e60f1d3e547cfe4e814c7f877 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:02:17 LN:3323 M5:3af3bf43df87b594bc20bc5f791785b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:02:27 LN:2872 M5:6ca96787a367bd1ce0838f941140cb81 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:03:01 LN:3323 M5:83f22de8d085ea321aad42dbf25a571a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:03:02 LN:2676 M5:9fcac4fbbe8fd92d125550cd06db12c1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:04 LN:3239 M5:9b24c6a9a01dea4a6bcdb76824470e3e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:09 LN:3317 M5:5ed7833ec37da80387b4a86c6b2a2040 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:138Q LN:3043 M5:1088ef8207c18405bc0b180f0226dab0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:150 LN:2676 M5:0290d7c3b24a19eefc6a89bc41146c39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:23N LN:3323 M5:f1ec2e8fa319def50d20ce89e0b80d5a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:26 LN:2804 M5:174005655892f2cff1ad41bb64927506 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:46 LN:3323 M5:4b352ce812b6c8a65a2e45741d805d7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:49 LN:3039 M5:cb75887e3f845944c90cdf5315369b16 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*44:56N LN:2676 M5:4da8a161653ca5951070a765b2070b3f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*45:01:01 LN:3338 M5:86f5f0263cbccdf31fc646079fce98d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*45:04 LN:3339 M5:bba751b5e3727ad2af0a8cb343a94d57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*46:01:01 LN:3336 M5:7ab2f1273efeb3a770ca80b5d0d078b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*46:01:05 LN:2891 M5:314e19fc403d8ea017669e53723aead0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*47:01:01:01 LN:3041 M5:3107f208d002db7e36cf098ebbb8516c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*47:01:01:02 LN:3041 M5:9f6a5f1290f5f00afcc6b1e732a37b14 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*48:01:01 LN:3323 M5:105f81689cb818d76fe1effd3d150157 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*48:03:01 LN:2676 M5:874fcca35339a027ede1021bb682c5b9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*48:04 LN:2676 M5:77accce2b51d36c34cfae7d7f77a0644 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*48:08 LN:3323 M5:f6f1a40b586aa5025bb2ed56a890ac8c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*49:01:01 LN:3340 M5:7da4db3c2933e38871dde7de2cdf0131 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*49:32 LN:3340 M5:1a762b6501a216624feb498212c99a07 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*50:01:01 LN:3340 M5:9d8a8957807527e6413e745a8fd4e068 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*51:01:01 LN:3327 M5:263006da7f87d352df017a5efcc6a2ad UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*51:01:02 LN:3043 M5:d0a5d20ecc047c9db286860c217ffe57 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*51:02:01 LN:3327 M5:0f09e1d79fda822bb499e29d59e7dfb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*51:07:01 LN:3327 M5:63b4b6ba9c4049e98e44399a1b1ad03c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*51:42 LN:2962 M5:8253b5b1e485378dfeedfa598e677579 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*52:01:01:01 LN:3327 M5:bb94101c60541202c4b844e21b640a92 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*52:01:01:02 LN:3327 M5:ff0ebc333c63692ac07e7b95c6febbd0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*52:01:01:03 LN:3327 M5:9e6abe5b1d3205aed3a8b62d580f5c6c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*52:01:02 LN:3327 M5:d7fde559163710e8a22f5497902dee7b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*53:01:01 LN:3327 M5:0e08f9e5e5ee052f077c388fe43ed672 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*53:11 LN:3274 M5:9d84a07be4be471551bc6c8cd89ddfa0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*54:01:01 LN:3332 M5:7621d4b27eef76b4b57df91eee3bd38c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*54:18 LN:2813 M5:ecf8408d4379ee7e2467c784e51ea2bb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:01:01 LN:3332 M5:b19860ce7d3e3feec3da15d850ffaf83 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:01:03 LN:3332 M5:17fe5fcacdc35ec18448d04e206578e2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:02:01 LN:3333 M5:fe9cf4bfff80e738fa573a1e4336621c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:12 LN:3332 M5:570fdcabb0ff55d30de3a319b09b2da2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:24 LN:3332 M5:0194f66b5128ba6d3eb9fec43ee985c7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*55:48 LN:2980 M5:b8cd7ebdabaea8025fe0b0308b1c500f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*56:01:01 LN:2688 M5:8ea679a7f814ff4b104970fc3363591c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*56:03 LN:2688 M5:13a5b854a9c6cf607e0758dd8977a136 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*56:04 LN:2688 M5:52674e8d83e65bf23b0b36f2cee3b279 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*57:01:01 LN:3337 M5:435c90dcaa816e70a0a07c23d2f363b7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*57:03:01 LN:2689 M5:b412ccacf520ef015be9010e88d13fd6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*57:06 LN:3284 M5:14711544ed455ec1aa9a55bae251514e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*57:11 LN:3306 M5:66b4e723563c1e4a79cc9233cbc2d5fb UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*57:29 LN:3337 M5:ae6a6681ba3a2ed0efeceafc6ac41fb7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*58:01:01 LN:3336 M5:89112836a876c731a21d3e2357e297d6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*58:31N LN:3004 M5:8507d0acf3a65b125d7ff5bd6c4d1e8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*59:01:01:01 LN:3333 M5:23fda5090f014267db805d001c7c3add UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*59:01:01:02 LN:3332 M5:c4602c638fb61927261e08d529f2898f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*67:01:01 LN:3312 M5:87814da89393ae312125f9b36af8bb77 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*67:01:02 LN:2675 M5:390a5500664173ba6708dbeed0e360dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*67:02 LN:3307 M5:ca5872a8e8c8fe4b920ba96e1542cf58 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*73:01 LN:3323 M5:0136d8cfbbe9fd393a1e699753f7f7a3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*78:01:01 LN:3327 M5:81fbcef3edbbc1e8df879a12d817a9b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*81:01 LN:2676 M5:06b880357cb8d04e7715100462e6e58d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-B*82:02:01 LN:3050 M5:7f0f011b72e32064bdeab6fc9d15df0f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:02:01 LN:3349 M5:0071e65ee429afd318ef699ad315c4b4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:02:11 LN:3057 M5:b9f8c9b73140b44e888ca0d3cb97a51a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:02:29 LN:3349 M5:daaad7b0fa23883b6f6a74c29b218154 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:02:30 LN:3333 M5:f72ada7c617959035065509827004c55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:03 LN:3349 M5:1dfba36de6a84343e0f1bc21f4290dd1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:06 LN:2895 M5:707a109a70808774faabaa9f20fc9c2d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:08 LN:3349 M5:d84d29f8412f7bc25c6faa02ce1e8027 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:14 LN:2895 M5:6184a7ce778825a8d3dbbdca08425352 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:21 LN:2895 M5:7c3c33a8a09cf7ab14dfb252dbb028be UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:30 LN:3349 M5:e7dc7b0b79ca3fba89e46935c2f6f53a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*01:40 LN:2968 M5:1f1ff8df1d52ca176768c131fa0dfd1b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:02:02:01 LN:3347 M5:7d432e207af5901819172d943367ab85 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:02:02:02 LN:3347 M5:3b927e5396f09d7b73cebc3cf7894022 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:10 LN:2893 M5:bc3caf3cb6c08beee025921ea8ab2c2b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:11 LN:3320 M5:86a83cd46a667da7f75c104e6964997a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:16:02 LN:3029 M5:117c55e59cdf735f8e4600c677295e86 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:69 LN:2933 M5:8fb20dc7ab2383635eb05c7b6aa75585 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:85 LN:3347 M5:19bc16298211d1df9285935c0e74349e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:86 LN:3347 M5:1a839f0d783dd916f4c4d85314fb33d3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*02:87 LN:3064 M5:efb30bed21ab99dd6db7b5aec573702f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:02:01 LN:2894 M5:df3f967e88fe534c02bfad024b8c887a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:02:02:01 LN:3348 M5:7ca03d45f7f1fd27eac518520148daa3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:02:02:02 LN:2896 M5:6d6e1775bedec2a59fa4d0bd7c94f4c5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:02:02:03 LN:3348 M5:22fe2d580ec4460d1b085eba87d9aac0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:03:01 LN:3348 M5:4e9e631972020eda52a012a3bcce4cd5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:04:01:01 LN:3348 M5:df0eaa78940580a0f21395f9387cc32d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:04:01:02 LN:3348 M5:d13c9657f578e20631d54771581b5204 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:04:02 LN:2877 M5:be42038dd5348757a9c683918b1db4f0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:04:04 LN:2966 M5:deeefe05be47eaf45bb483add4913ffc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:05 LN:2894 M5:bb0c05e7fd6c15d9d6593a4f3bbe783f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:06 LN:2894 M5:21c18b0c0ff10700610d7f1a50b1c899 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:100 LN:3034 M5:99e13ee46eceaa60fe5084f320894a2d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:13:01 LN:3065 M5:3981907aafcbb510b01423d7f323477c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:20N LN:3321 M5:b3a7cb20db02d1256c2106d420e765fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:219 LN:3070 M5:2f9e694079fd1378d796e05baa51889c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:261 LN:3348 M5:3248cc886e5d3793ba099f0ab1a05d66 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:40:01 LN:2894 M5:93c62f86d2dfc6633dbd2c975908da1a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:41:02 LN:3328 M5:f5752e34d19b00f37d306621c38909a2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:46 LN:2997 M5:0ea8327e0b67ad6f90e5a44c1629c7d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*03:61 LN:2894 M5:6d712f749acb251535e2ae8c3c617413 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:01:01 LN:3349 M5:3efce3936c70251730ad6b287f7d110e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:01:02 LN:3349 M5:5d4e1f36ad16f12cbf6447e60f24081c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:01:03 LN:3349 M5:9e9f6956c08e23d56266b6fa217efd7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:01:04 LN:3012 M5:2579591d3a321b72379ecd8a51d7edd7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:01:05 LN:2931 M5:64ad58c15cabe00bc7528c32341c0878 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:01:62 LN:3329 M5:43792d77155a7b8f48cd4c57e217997e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:03:01 LN:3349 M5:0ccb8d21235af6c2aff22aa6afbc6732 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:06 LN:3349 M5:63b57fd97300edc439359a50449974db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:09N LN:2991 M5:0fbbbf7cb8fddeb130cf9e967404f973 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:128 LN:3086 M5:e6d5cc6672b9d7e2050ce659e5ed35b6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:161 LN:3237 M5:5bf40c2edbc86b4e13300af3d685dc1b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:177 LN:3349 M5:8547dcf17572b7586eec8ec28cbb8792 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:70 LN:3058 M5:5f1c7d679cd5c3f3110db5fe628cd613 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*04:71 LN:3086 M5:02b46fcce463bf80c626c51f5d36bab3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*05:01:01:01 LN:3349 M5:c6d922fe90d58a4380b338ce33a098db UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*05:01:01:02 LN:3349 M5:a7b54d490750d2723ab72f5eab4de589 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*05:08 LN:3059 M5:1560938f348bbf8027a77fe08ce4b46f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*05:09:01 LN:3322 M5:bb8835a101a1bd226ba1cf04e30c8298 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*05:93 LN:2946 M5:8163cb61a36ebacb034b44d4537e3ccd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:02:01:01 LN:3349 M5:e2d2d51306ffa6a15072abc9e218afa3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:02:01:02 LN:3349 M5:289ade2a84e2a669f4950d905de18ef3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:02:01:03 LN:3349 M5:541d1cf5b93596d04882fdcea98fdb24 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:23 LN:3349 M5:f90da72a0371a17e345090ee35ced67e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:24 LN:3349 M5:8416842ec1691954a14211e9b178951b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*06:46N LN:2987 M5:1276d9ecb257086ba85fb1dff231b2d1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:01:01 LN:3354 M5:718bc83515052da25d2aa2cf1017aa8d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:01:02 LN:3093 M5:e45eed48ec341cc12ae10b917381d885 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:02 LN:3352 M5:d7dd73d1eb5b9a25c83e84ef950dd44b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:19 LN:3354 M5:db13dc42d8ac6f58daa4d04dd21e0296 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:27 LN:3195 M5:53fc59605c057f246aa7cb06caa3523c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:01:45 LN:3354 M5:7b104b85dc1b908764ff4904a903c90a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:01:01 LN:3354 M5:00b5a7cce8e5acc98b508e4d39091f15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:01:02 LN:3074 M5:152f1c93813d70470aa3a116478c5a69 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:01:03 LN:3354 M5:b854cfa857b40775c2ad6e6ded4b848a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:01:04 LN:3353 M5:7de039797ea7911c70f4fa82c658366e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:01:05 LN:3354 M5:00709b8f2f15aa3aa482a992d1d87b60 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:05 LN:2903 M5:c7cbc76d1fb0114a55ee635afdb0cd15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:06 LN:3354 M5:1d0e73f11030eece15f033cab7216c51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:02:64 LN:3354 M5:7f780958705ae801a5814f2471cb59f4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:04:01 LN:3354 M5:7aa1a4c1a413aca4e232a2e7dce8ee4d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:04:02 LN:3343 M5:b44a66a5ff9ba711c465dc629d4336e9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:06 LN:3354 M5:3ac9135bdf1975f31d5f501cb5b62c98 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:149 LN:3098 M5:df315cb1e89fca70587836e750b11737 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:18 LN:3353 M5:fa6f988acdf9509ba3790814e29ef79c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:19 LN:3222 M5:2597c84fe3afb1831211a4c9ad6427ec UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:26 LN:3069 M5:705cb7c1e6112114a894aab34b391fdf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:30 LN:2903 M5:e5947b700176aabf378cf29e78c17084 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:32N LN:3334 M5:fe7d565923067a8248624f41f45c79df UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:384 LN:3349 M5:90a8a5267a890ccd78717e88de59232d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:385 LN:3354 M5:20b5d8a093d1e81d1e6b9e56b6d4ad17 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:386 LN:3183 M5:b5524ee2cc10b5a45e99af42e190f5f3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:391 LN:3354 M5:de98c716203e1ca551130af6affe2199 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:392 LN:3354 M5:1a509719976de84a60b59343364e4b51 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:49 LN:2935 M5:45a4d818fffa2d2428658489af9e98b1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:56:02 LN:3354 M5:e4c99b9226de6adbf587934d835048d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:66 LN:3354 M5:a822c754dee47e720af532f5f5bb1ef4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*07:67 LN:3354 M5:2ba41a6538775c77f84b648069196d70 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:01:01 LN:3349 M5:96d5d92a1f9a605bb014f058b767ecfd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:01:03 LN:2998 M5:585c1deb75a38ddadc48575546be3772 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:02:01:01 LN:3349 M5:734e931f1481380f2d0bf98c7925ee2f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:02:01:02 LN:3349 M5:8f2c108d077649a22ae5df5fb42fe265 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:03:01 LN:3349 M5:633f446c1fba5006f49dd2bbddb3ce5e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:04:01 LN:2895 M5:0d4f700e3c6bb58e12ab10c1c85f1bcc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:112 LN:3178 M5:ad581d3ae2ddf7d3895c8bda459221c3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:20 LN:3349 M5:de321b1690b9ce4e07c6f6f1c838ba7c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:21 LN:3349 M5:574d8b3a969397fe0d6168a851acac10 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:22 LN:3349 M5:c4fc4ff7544492d91fc221e274b790a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:24 LN:2895 M5:9cc0d743f4c6cea3a61dd94cf81e8782 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:27 LN:3349 M5:3ab94e3b0cb8b613e8cc644c6dc715d4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:36N LN:3097 M5:5e4997d79367c75ea3301d1222f70938 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:40 LN:2978 M5:e8013eaab611499b1827191279b34223 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:41 LN:3019 M5:bf7ecc6786f26c3740e9291e7fdd990e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*08:62 LN:3086 M5:a3f951c6e67ba7b5d43b4678504ceb8f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:02:02 LN:3349 M5:6f12358794b5c624b6948b27722c0d4d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:03:01:01 LN:3349 M5:a5f9f1324e2a617a08c5bcbc275608b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:03:01:02 LN:3348 M5:ff3c56a68534ef6adbf0622ea691498c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:08 LN:3066 M5:5800471074ab27c8bea70d68c5e225d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:13 LN:3058 M5:807b94f9d89314085415b5578b24a778 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:19 LN:3349 M5:3f11cc62c9ed887231ac88ad834d72c9 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:22 LN:2895 M5:9ba9eb10b465c567357944d23d28cb83 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*12:99 LN:3349 M5:6bc65113a76be4e417061df97ec9323e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*14:02:01 LN:3349 M5:8707b7806173ec4a4c61b1d405f1aae3 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*14:03 LN:3349 M5:833afc09e1ba23f97925580cbd67b077 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*14:21N LN:3099 M5:360ab26c5e5b147d6195bcdff0c92871 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*14:23 LN:2976 M5:e5e65cbe62521c667b3ed587aaa762e0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:02:01 LN:3349 M5:95c60c535112b121a9e6ee900399d619 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:05:01 LN:3349 M5:d59cc60db3e774256fafc8cb4e13702e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:05:02 LN:3349 M5:e4ecf0c60d3ee668e494d70c54964c29 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:13 LN:2895 M5:a7312985b9c4182c9232eb0ffdc57ed0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:16 LN:3066 M5:35043b5832829f2204f9418cc925e19b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:17 LN:3349 M5:82cd4aaf3336e9d3414b28f0d7286e9b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*15:96Q LN:3349 M5:42133cdaef4b18738bc26fa5695f8f65 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*16:01:01 LN:3349 M5:0c9b3216f1f00e1a3201ea90fb49bd61 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*16:02:01 LN:2895 M5:cf1da0837be9b9302b0fb7158fc5396d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*16:04:01 LN:3349 M5:aefc6289675459afe38397002b7451c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*17:01:01:01 LN:3368 M5:aafe636c5bfce4449d6eed73eefa3a9f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*17:01:01:02 LN:3368 M5:d3cbf8067c1957eaeb80c6a399477245 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*17:01:01:03 LN:3368 M5:f2cc1e59a91a4f102f9ab5fdf4d3c1ff UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*17:03 LN:3197 M5:a94688722b11045bbf7ef13dfc7d34a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-C*18:01 LN:3346 M5:d53dd9ee731f0437bbbffc584867b0b5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:01:02 LN:6489 M5:d0d728fb8e62a0b0366517669b0e4164 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:02:01:01 LN:6484 M5:823632b556203973124fe9c35cb13a3b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:02:01:02 LN:6485 M5:6d2b0b5b4de67207756f9a9ca9132232 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:02:01:03 LN:6485 M5:df8eab835a0677224be043db0196865c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:02:01:04 LN:6492 M5:1e79553d8e925c6243368db955220c7d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:03:01:01 LN:6485 M5:705ec2d75d9d349a7e4d1b2df73073d7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:03:01:02 LN:6492 M5:dc4b199889931d13d1522358a9a01f3c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:04:01:01 LN:6484 M5:f9a28fc80725b04a807c1a7fa37786bf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:04:01:02 LN:6485 M5:da201e2fb04103e9cfa1eaecab78b098 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:05:01 LN:6485 M5:a13023aaec38d48c163c5fe5996596d8 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:07 LN:5959 M5:1af58658f072dc6319086af52c3d5f6f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:10 LN:5790 M5:52685ee8463a30a3909841a38e4b9d8b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*01:11 LN:5926 M5:18894dd38987f5ffd32b9896a4a7f732 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*02:01 LN:6403 M5:14bb0fb46e443150199a75018f001115 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*03:01:01 LN:6437 M5:75b42b5c4fb93885ab4fdb6a312d690f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*03:02 LN:6437 M5:caaf9e4318294ebd0c262db0018d88fa UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*03:03:01 LN:6437 M5:c0908bb87356f6cad982be1acb618dcc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*04:01:02:01 LN:5853 M5:dc7c3223c9df7e828a1c8f22c4cc89c4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*04:01:02:02 LN:5666 M5:6548c257283705fae841fe89148efc1f UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*04:02 LN:6210 M5:6d9b8e4e332e7e398b831c81a6179e76 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:01:01:01 LN:5806 M5:72dbcba71cbed79962e1fcb9efa94824 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:01:01:02 LN:6529 M5:b129bfcbf4b80c10ffab12eb652ba5a1 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:03 LN:6121 M5:38e52c5a505ee662100c0789cfe884a7 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:05:01:01 LN:6593 M5:f4020b6d26ac50eed22026f28371f610 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:05:01:02 LN:6597 M5:456ddd5aaccb336023a80f2ae5abbbdd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:05:01:03 LN:6393 M5:39e340eb675634315a0f9aa27e8fdb32 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*05:11 LN:6589 M5:a47aa87bace1ec4da327ee121bceac36 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQA1*06:01:01 LN:5878 M5:cd02d6c8bd4c86f9abb999bab52820cf UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*02:01:01 LN:7480 M5:0b0031da03392e56acd208fc67ae6e39 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*02:02:01 LN:7471 M5:69337da3c9bd0a6048d1a1144aabbdb0 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*03:01:01:01 LN:7231 M5:78802163e98cdc6f909438b62e0cfab4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*03:01:01:02 LN:7230 M5:e8f01ef987d6dd2a878f94e6d97be1f1 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LN:7090 M5:0f304adf7acf3bd4b7c54c1394c85a4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*05:03:01:01 LN:7089 M5:618b80618d0a6750a82d9caf5b2b01ea UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*05:03:01:02 LN:7089 M5:198d06518fff03b2dafc0bb113efc008 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*06:01:01 LN:7111 M5:231c9c7d1321a266933777181a2761a4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*06:02:01 LN:7102 M5:739d56a7e8c44823d1a11bd4ff13f465 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*06:03:01 LN:7103 M5:1bfde4e12c5bac5e9e5e7e6b84582b0b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DQB1*06:09:01 LN:7102 M5:805267e877e03b8ec95319888e38c5dc UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*01:01:01 LN:10741 M5:91618decb1b3933c623070b2ccbe52a6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*01:02:01 LN:11229 M5:9efa1a8356e55f6be83b4ddb84833e4b UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*03:01:01:01 LN:13908 M5:411919f8128149a1f5099cdd22d276ab UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*03:01:01:02 LN:13426 M5:869ae29cc3f279d7fc43e27249a53e8a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*04:03:01 LN:15246 M5:ce0de8afd561fb1fb0d3acce94386a27 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*07:01:01:01 LN:16110 M5:4063054a8189fbc81248b0f37b8273fd UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*07:01:01:02 LN:16120 M5:a4b1a49cfe8fb2c98c178c02b6c64ed4 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*08:03:02 LN:13562 M5:dc6eb484555ba796b15b9b91aa1a9ec6 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*09:21 LN:16039 M5:cad793cbc22699d1cca3101b5a55437d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*10:01:01 LN:13501 M5:91dcd8c149b00a3edb1afa43a1cc6346 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*11:01:01 LN:13921 M5:07cefc23572c21731a2804c5d44fcf55 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*11:01:02 LN:13931 M5:f47cd478ee5be3ff048069adbbb5c101 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*11:04:01 LN:13919 M5:fc86714e3d7b9503d74e40d92f08cd7a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*12:01:01 LN:13404 M5:31f08980f62b68be96334f3cafc3e7c2 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*12:17 LN:11260 M5:b0b7bea536479c8e61164e1475203038 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*13:01:01 LN:13935 M5:937994edc4b4760eb13cbf451c3f3bbe UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*13:02:01 LN:13941 M5:6a124566ab0a103d525d2fcf1292ce15 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*14:05:01 LN:13933 M5:10890137f738be0861b6ba128cfb953d UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*14:54:01 LN:13936 M5:2b67be4ed005b3f3040e3c72bfc65c0c UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:01:01:01 LN:11080 M5:d22cd7f323b0e51abb025a4dec827aa5 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:01:01:02 LN:11571 M5:5e3280587aa6f38dd9a282e9bf21287e UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:01:01:03 LN:11056 M5:d548f5e5798e09c869571ea8c660c983 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:01:01:04 LN:11056 M5:3bb2753595aaf099a490a7ce1e042fce UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:02:01 LN:10313 M5:2deab6035ce8cc2e09db20698cf1f117 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:03:01:01 LN:11567 M5:e6a26b767a62b282e0db211bbc9a7363 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*15:03:01:02 LN:11569 M5:4e0d459b9bd15bff8645de84334e3d25 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa +@SQ SN:HLA-DRB1*16:02:01 LN:11005 M5:4a972df76bd3ee2857b87bd5be5ea00a UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa chr1 10001 207666 + . chr1 257667 297968 + . chr1 347969 535988 + . diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list index 8b2defac..fc515fb0 100644 --- a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list +++ b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/chr14_non_gap_regions.interval_list @@ -1,5 +1,5 @@ @HD VN:1.6 SO:coordinate -@SQ SN:chr14 LN:107043718 M5:e0f0eecc3bcab6178c62b6211565c807 UR:file:/Users/gnanakkan/NPM-sample-qc/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/work/d7/208435b73576bd9dd7cc1a11c12341/hg38.fa +@SQ SN:chr14 LN:107043718 M5:e0f0eecc3bcab6178c62b6211565c807 UR:s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa chr14 16000001 16022537 + . chr14 16022638 16053976 + . chr14 16054460 16061677 + . From 9d1441c0f7d88d9851156c82508f8c1a7f4321f6 Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 14:24:58 +0800 Subject: [PATCH 130/132] Update few sections in the README --- README.rst | 21 ++++++++++----------- 1 file changed, 10 insertions(+), 11 deletions(-) diff --git a/README.rst b/README.rst index 31fec358..01f8e892 100644 --- a/README.rst +++ b/README.rst @@ -45,7 +45,7 @@ Run workflow on 45Mbp region around AKT1 gene, 30X, of sample NA12878 from the 1 This creates `output` directory with the results that can be compared to the content of `output_certified` :: - diff output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json output/results/multiqc/NA12878-chr14-AKT1.metrics.json + diff output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json output/results/metrics/NA12878-chr14-AKT1.metrics.json Please refer to the workflow help for more information on its usage and access to additional options: :: @@ -59,7 +59,7 @@ Resources The workflow requires the following resources given in the ``conf/resources.config`` -- *N-regions reference file*, used as an input for computing "non-gap regions autosome" coverages (mosdepth). +- *N-regions reference file*, used as an input for computing "non-gap regions autosome" coverages (picard, bcftools). - Gaps in the GRCh38 (hg38) genome assembly, defined in the AGP file delivered with the sequence, are being closed during the finishing process on the human genome. GRCh38 (hg38) genome assembly still contains the following principal types of gaps: @@ -75,6 +75,8 @@ The workflow requires the following resources given in the ``conf/resources.conf - *FASTA file index*. This file can be downloaded from ``s3://1000genomes-dragen-3.7.6/references/fasta/hg38.fa.fai`` and not required to be specified in the config. The workflow will look fasta index file in a folder the fasta file is present. +- *Verify Bam ID 2 reference panel files*, 100K sites from 1000 Genome Project phase 3 build 38, downloaded from ``https://github.com/Griffan/VerifyBamID/tree/master/resource/``. + Inputs ------ @@ -100,14 +102,15 @@ Upon completion, the workflow will create the following files in the ``outdir`` timeline.html trace.txt results/ # final metrics.json and intermediate outputs - mosdepth/ - multiqc/ + bcftools/ + metrics/ .metrics.json picard_collect_multiple_metrics/ + picard_collect_wgs_metrics/ samtools/ verifybamid2/ -If ``keep_workdir`` has been specified, the contents of the Nextflow work directory (``work-dir``) will also be preserved. +If ``cleanup = true`` in the nextflow.config is commented out, the contents of the Nextflow work directory (``work-dir``) will also be preserved. Docker image ------------ @@ -132,15 +135,11 @@ In a nutshell, this workflow generates QC metrics from single-sample WGS results **Metrics calculation** -The current workflow combines widely-used third-party tools (samtools, picard, mosdepth) and custom scripts. Full details on which processes are run/when can be found in the actual workflow definition (``main.nf``). We also provide an example dag for a more visual representation (``tests/NA12878_1000genomes-dragen-3.7.6/dag.pdf``). +The current workflow combines widely-used third-party tools (samtools, picard, bcftools, verifybamid2) and custom scripts. Full details on which processes are run/when can be found in the actual workflow definition (``main.nf``). We also provide an example dag for a more visual representation (``tests/NA12878_1000genomes-dragen-3.7.6/dag.pdf``). **Metrics parsing** -Next, output files from each individual tool are parsed and combined into a single json file. This is done by calling ``bin/multiqc_plugins/multiqc_npm/``, a MultiQC plugin that extends the base tool to support additional files. - -**Metrics reporting** - -Finally, the contents of the MultiQC json are formatted into a final metrics report, also in json format. The reporting logic lives in the ``bin/compile_metrics.py`` script, and whilst its contents are simple, it enables automatic documentation of metric definitions from code comments (see the **Metric definitions** section). +Next, output files from each individual tool are parsed and combined into a single json file. Metric definitions ================== From fcf0da8906bfeeb9b5fdfc7da3b8e79589927c3c Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 14:45:54 +0800 Subject: [PATCH 131/132] Switch Dockerfile git clone source --- containers/Dockerfile | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/containers/Dockerfile b/containers/Dockerfile index 226956ac..e93152ec 100644 --- a/containers/Dockerfile +++ b/containers/Dockerfile @@ -45,10 +45,10 @@ RUN conda env create -f /conda_env.yml RUN conda clean -a # Clone master -# RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git +RUN git clone --depth 1 https://github.com/c-BIG/NPM-sample-qc.git # Clone develop -RUN git clone -b develop https://github.com/c-BIG/NPM-sample-qc.git +# RUN git clone -b develop https://github.com/c-BIG/NPM-sample-qc.git WORKDIR /NPM-sample-qc/bin RUN pip3 install numpy scipy argparse From 6d121bb3828cdd08d4bfc1186649503ebf99db44 Mon Sep 17 00:00:00 2001 From: mhebrard Date: Wed, 25 Sep 2024 14:47:19 +0800 Subject: [PATCH 132/132] Update doc --- .../api/metrics.count_deletions.rst.txt | 6 + .../api/metrics.count_insertions.rst.txt | 6 + docs/_sources/api/metrics.count_snvs.rst.txt | 6 + ...ratio_heterozygous_homzygous_indel.rst.txt | 6 + ...s.ratio_heterozygous_homzygous_snv.rst.txt | 6 + .../metrics.ratio_insertion_deletion.rst.txt | 6 + ...cs.ratio_transitions_transversions.rst.txt | 6 + docs/api/metrics.count_deletions.html | 132 ++++++++++++++++++ docs/api/metrics.count_insertions.html | 132 ++++++++++++++++++ docs/api/metrics.count_snvs.html | 132 ++++++++++++++++++ .../api/metrics.cross_contamination_rate.html | 11 +- docs/api/metrics.html | 49 +++++++ .../metrics.insert_size_std_deviation.html | 7 + docs/api/metrics.mad_autosome_coverage.html | 7 + docs/api/metrics.mean_autosome_coverage.html | 7 + docs/api/metrics.mean_insert_size.html | 7 + docs/api/metrics.pct_autosomes_15x.html | 7 + docs/api/metrics.pct_reads_mapped.html | 7 + .../metrics.pct_reads_properly_paired.html | 11 +- ...cs.ratio_heterozygous_homzygous_indel.html | 132 ++++++++++++++++++ ...rics.ratio_heterozygous_homzygous_snv.html | 132 ++++++++++++++++++ .../api/metrics.ratio_insertion_deletion.html | 132 ++++++++++++++++++ ...trics.ratio_transitions_transversions.html | 132 ++++++++++++++++++ docs/api/metrics.yield_bp_q30.html | 11 +- docs/genindex.html | 53 +++++++ docs/index.html | 22 +-- docs/metrics.html | 50 +++++-- docs/objects.inv | Bin 578 -> 769 bytes docs/searchindex.js | 2 +- 29 files changed, 1188 insertions(+), 29 deletions(-) create mode 100644 docs/_sources/api/metrics.count_deletions.rst.txt create mode 100644 docs/_sources/api/metrics.count_insertions.rst.txt create mode 100644 docs/_sources/api/metrics.count_snvs.rst.txt create mode 100644 docs/_sources/api/metrics.ratio_heterozygous_homzygous_indel.rst.txt create mode 100644 docs/_sources/api/metrics.ratio_heterozygous_homzygous_snv.rst.txt create mode 100644 docs/_sources/api/metrics.ratio_insertion_deletion.rst.txt create mode 100644 docs/_sources/api/metrics.ratio_transitions_transversions.rst.txt create mode 100644 docs/api/metrics.count_deletions.html create mode 100644 docs/api/metrics.count_insertions.html create mode 100644 docs/api/metrics.count_snvs.html create mode 100644 docs/api/metrics.ratio_heterozygous_homzygous_indel.html create mode 100644 docs/api/metrics.ratio_heterozygous_homzygous_snv.html create mode 100644 docs/api/metrics.ratio_insertion_deletion.html create mode 100644 docs/api/metrics.ratio_transitions_transversions.html diff --git a/docs/_sources/api/metrics.count_deletions.rst.txt b/docs/_sources/api/metrics.count_deletions.rst.txt new file mode 100644 index 00000000..48746442 --- /dev/null +++ b/docs/_sources/api/metrics.count_deletions.rst.txt @@ -0,0 +1,6 @@ +count_deletions +=============== + +.. currentmodule:: metrics + +.. autofunction:: count_deletions diff --git a/docs/_sources/api/metrics.count_insertions.rst.txt b/docs/_sources/api/metrics.count_insertions.rst.txt new file mode 100644 index 00000000..003935bb --- /dev/null +++ b/docs/_sources/api/metrics.count_insertions.rst.txt @@ -0,0 +1,6 @@ +count_insertions +================ + +.. currentmodule:: metrics + +.. autofunction:: count_insertions diff --git a/docs/_sources/api/metrics.count_snvs.rst.txt b/docs/_sources/api/metrics.count_snvs.rst.txt new file mode 100644 index 00000000..32178d28 --- /dev/null +++ b/docs/_sources/api/metrics.count_snvs.rst.txt @@ -0,0 +1,6 @@ +count_snvs +========== + +.. currentmodule:: metrics + +.. autofunction:: count_snvs diff --git a/docs/_sources/api/metrics.ratio_heterozygous_homzygous_indel.rst.txt b/docs/_sources/api/metrics.ratio_heterozygous_homzygous_indel.rst.txt new file mode 100644 index 00000000..58e5e4a9 --- /dev/null +++ b/docs/_sources/api/metrics.ratio_heterozygous_homzygous_indel.rst.txt @@ -0,0 +1,6 @@ +ratio_heterozygous_homzygous_indel +================================== + +.. currentmodule:: metrics + +.. autofunction:: ratio_heterozygous_homzygous_indel diff --git a/docs/_sources/api/metrics.ratio_heterozygous_homzygous_snv.rst.txt b/docs/_sources/api/metrics.ratio_heterozygous_homzygous_snv.rst.txt new file mode 100644 index 00000000..c43a8d34 --- /dev/null +++ b/docs/_sources/api/metrics.ratio_heterozygous_homzygous_snv.rst.txt @@ -0,0 +1,6 @@ +ratio_heterozygous_homzygous_snv +================================ + +.. currentmodule:: metrics + +.. autofunction:: ratio_heterozygous_homzygous_snv diff --git a/docs/_sources/api/metrics.ratio_insertion_deletion.rst.txt b/docs/_sources/api/metrics.ratio_insertion_deletion.rst.txt new file mode 100644 index 00000000..32046132 --- /dev/null +++ b/docs/_sources/api/metrics.ratio_insertion_deletion.rst.txt @@ -0,0 +1,6 @@ +ratio_insertion_deletion +======================== + +.. currentmodule:: metrics + +.. autofunction:: ratio_insertion_deletion diff --git a/docs/_sources/api/metrics.ratio_transitions_transversions.rst.txt b/docs/_sources/api/metrics.ratio_transitions_transversions.rst.txt new file mode 100644 index 00000000..e81c9f87 --- /dev/null +++ b/docs/_sources/api/metrics.ratio_transitions_transversions.rst.txt @@ -0,0 +1,6 @@ +ratio_transitions_transversions +=============================== + +.. currentmodule:: metrics + +.. autofunction:: ratio_transitions_transversions diff --git a/docs/api/metrics.count_deletions.html b/docs/api/metrics.count_deletions.html new file mode 100644 index 00000000..c0c4fceb --- /dev/null +++ b/docs/api/metrics.count_deletions.html @@ -0,0 +1,132 @@ + + + + + + + count_deletions — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
              + + +
              + +
              +
              +
              + +
              +
              +
              +
              + +
              +

              count_deletions

              +
              +
              +metrics.count_deletions(mqc, biosample_id)
              +

              Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

              +

              Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS/bcftools view -H -v indels -f PASS….DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

              +
              + +
              + + +
              +
              + +
              +
              +
              +
              + + + + \ No newline at end of file diff --git a/docs/api/metrics.count_insertions.html b/docs/api/metrics.count_insertions.html new file mode 100644 index 00000000..0459a9ed --- /dev/null +++ b/docs/api/metrics.count_insertions.html @@ -0,0 +1,132 @@ + + + + + + + count_insertions — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
              + + +
              + +
              +
              +
              + +
              +
              +
              +
              + +
              +

              count_insertions

              +
              +
              +metrics.count_insertions(mqc, biosample_id)
              +

              Description: The number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants.

              +

              Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS). Insertions are only considered in this metric short, less than 50bp, insertions as commonly identified by most short reads variant callers. Structural variations which include insertions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

              +
              + +
              + + +
              +
              + +
              +
              +
              +
              + + + + \ No newline at end of file diff --git a/docs/api/metrics.count_snvs.html b/docs/api/metrics.count_snvs.html new file mode 100644 index 00000000..22f2f091 --- /dev/null +++ b/docs/api/metrics.count_snvs.html @@ -0,0 +1,132 @@ + + + + + + + count_snvs — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
              + + +
              + +
              +
              +
              + +
              +
              +
              +
              + +
              +

              count_snvs

              +
              +
              +metrics.count_snvs(mqc, biosample_id)
              +

              Description: The number of variant type SNVs in VCF, only in autosomal regions, high quality variants.

              +

              Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view. (bcftools view -H -v snps -f PASS)

              +
              + +
              + + +
              +
              + +
              +
              +
              +
              + + + + \ No newline at end of file diff --git a/docs/api/metrics.cross_contamination_rate.html b/docs/api/metrics.cross_contamination_rate.html index 39f4492a..4c664bb6 100644 --- a/docs/api/metrics.cross_contamination_rate.html +++ b/docs/api/metrics.cross_contamination_rate.html @@ -21,7 +21,7 @@ - + @@ -42,6 +42,9 @@

              Metric definitions

              • Functions
                • @@ -95,7 +102,7 @@

                cross_contamination_rate - + diff --git a/docs/api/metrics.html b/docs/api/metrics.html index dd1c836f..7c38d738 100644 --- a/docs/api/metrics.html +++ b/docs/api/metrics.html @@ -73,6 +73,27 @@

                Submodules

                metrics.metrics module

                +
                +
                +metrics.metrics.count_deletions(mqc, biosample_id)
                +

                Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS/bcftools view -H -v indels -f PASS….DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

                +
                + +
                +
                +metrics.metrics.count_insertions(mqc, biosample_id)
                +

                Description: The number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS). Insertions are only considered in this metric short, less than 50bp, insertions as commonly identified by most short reads variant callers. Structural variations which include insertions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

                +
                + +
                +
                +metrics.metrics.count_snvs(mqc, biosample_id)
                +

                Description: The number of variant type SNVs in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the number of variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view. (bcftools view -H -v snps -f PASS)

                +
                +
                metrics.metrics.cross_contamination_rate(mqc, biosample_id)
                @@ -129,6 +150,34 @@

                Submodules +
                +metrics.metrics.ratio_heterozygous_homzygous_indel(mqc, biosample_id)
                +

                Description: The ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS -g het / bcftools view -H -v indels -f PASS -g hom).

                +

                + +
                +
                +metrics.metrics.ratio_heterozygous_homzygous_snv(mqc, biosample_id)
                +

                Description: The ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v snps -f PASS -g het / bcftools view -H -v snps -f PASS -g hom).

                +
                + +
                +
                +metrics.metrics.ratio_insertion_deletion(mqc, biosample_id)
                +

                Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS/bcftools view -H -v indels -f PASS….DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

                +
                + +
                +
                +metrics.metrics.ratio_transitions_transversions(mqc, biosample_id)
                +

                Description: The ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality variants.

                +

                Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality-variants by bcftools stats, (bcftools stats -f PASS … TSTV).

                +
                +
                metrics.metrics.yield_bp_q30(mqc, biosample_id)
                diff --git a/docs/api/metrics.insert_size_std_deviation.html b/docs/api/metrics.insert_size_std_deviation.html index 1330f9fe..6c728946 100644 --- a/docs/api/metrics.insert_size_std_deviation.html +++ b/docs/api/metrics.insert_size_std_deviation.html @@ -42,6 +42,9 @@

                Metric definitions

                • Functions
                  • diff --git a/docs/api/metrics.mad_autosome_coverage.html b/docs/api/metrics.mad_autosome_coverage.html index 6ad07bea..c5167b50 100644 --- a/docs/api/metrics.mad_autosome_coverage.html +++ b/docs/api/metrics.mad_autosome_coverage.html @@ -42,6 +42,9 @@

                  Metric definitions

                  • Functions
                    • diff --git a/docs/api/metrics.mean_autosome_coverage.html b/docs/api/metrics.mean_autosome_coverage.html index e23fdad1..cd1e479e 100644 --- a/docs/api/metrics.mean_autosome_coverage.html +++ b/docs/api/metrics.mean_autosome_coverage.html @@ -42,6 +42,9 @@

                    Metric definitions

                    • Functions
                      • diff --git a/docs/api/metrics.mean_insert_size.html b/docs/api/metrics.mean_insert_size.html index 20dfcb0f..d94f2d2e 100644 --- a/docs/api/metrics.mean_insert_size.html +++ b/docs/api/metrics.mean_insert_size.html @@ -42,6 +42,9 @@

                      Metric definitions

                      • Functions
                        • diff --git a/docs/api/metrics.pct_autosomes_15x.html b/docs/api/metrics.pct_autosomes_15x.html index 063a534c..11412fd1 100644 --- a/docs/api/metrics.pct_autosomes_15x.html +++ b/docs/api/metrics.pct_autosomes_15x.html @@ -42,6 +42,9 @@

                        Metric definitions

                        • Functions
                          • diff --git a/docs/api/metrics.pct_reads_mapped.html b/docs/api/metrics.pct_reads_mapped.html index e1ac7687..751feded 100644 --- a/docs/api/metrics.pct_reads_mapped.html +++ b/docs/api/metrics.pct_reads_mapped.html @@ -42,6 +42,9 @@

                          Metric definitions

                          • Functions
                            • diff --git a/docs/api/metrics.pct_reads_properly_paired.html b/docs/api/metrics.pct_reads_properly_paired.html index d07cb196..310418a6 100644 --- a/docs/api/metrics.pct_reads_properly_paired.html +++ b/docs/api/metrics.pct_reads_properly_paired.html @@ -20,7 +20,7 @@ - + @@ -42,6 +42,9 @@

                            Metric definitions

                            • Functions
                              • @@ -96,7 +103,7 @@

                              pct_reads_properly_paired - +
                              diff --git a/docs/api/metrics.ratio_heterozygous_homzygous_indel.html b/docs/api/metrics.ratio_heterozygous_homzygous_indel.html new file mode 100644 index 00000000..1f5d3aaf --- /dev/null +++ b/docs/api/metrics.ratio_heterozygous_homzygous_indel.html @@ -0,0 +1,132 @@ + + + + + + + ratio_heterozygous_homzygous_indel — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
                              + + +
                              + +
                              +
                              +
                              + +
                              +
                              +
                              +
                              + +
                              +

                              ratio_heterozygous_homzygous_indel

                              +
                              +
                              +metrics.ratio_heterozygous_homzygous_indel(mqc, biosample_id)
                              +

                              Description: The ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants.

                              +

                              Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS -g het / bcftools view -H -v indels -f PASS -g hom).

                              +
                              + +
                              + + +
                              +
                              + +
                              +
                              +
                              +
                              + + + + \ No newline at end of file diff --git a/docs/api/metrics.ratio_heterozygous_homzygous_snv.html b/docs/api/metrics.ratio_heterozygous_homzygous_snv.html new file mode 100644 index 00000000..6b35e5ca --- /dev/null +++ b/docs/api/metrics.ratio_heterozygous_homzygous_snv.html @@ -0,0 +1,132 @@ + + + + + + + ratio_heterozygous_homzygous_snv — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
                              + + +
                              + +
                              +
                              +
                              + +
                              +
                              +
                              +
                              + +
                              +

                              ratio_heterozygous_homzygous_snv

                              +
                              +
                              +metrics.ratio_heterozygous_homzygous_snv(mqc, biosample_id)
                              +

                              Description: The ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants.

                              +

                              Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v snps -f PASS -g het / bcftools view -H -v snps -f PASS -g hom).

                              +
                              + +
                              + + +
                              +
                              + +
                              +
                              +
                              +
                              + + + + \ No newline at end of file diff --git a/docs/api/metrics.ratio_insertion_deletion.html b/docs/api/metrics.ratio_insertion_deletion.html new file mode 100644 index 00000000..5d85023d --- /dev/null +++ b/docs/api/metrics.ratio_insertion_deletion.html @@ -0,0 +1,132 @@ + + + + + + + ratio_insertion_deletion — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
                              + + +
                              + +
                              +
                              +
                              + +
                              +
                              +
                              +
                              + +
                              +

                              ratio_insertion_deletion

                              +
                              +
                              +metrics.ratio_insertion_deletion(mqc, biosample_id)
                              +

                              Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

                              +

                              Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of insertions and deletion in VCF, only in autosomal regions, high quality variants by bcftools view, (bcftools view -H -v indels -f PASS….INS/bcftools view -H -v indels -f PASS….DEL). Insertions and Deletions are only considered in this metric short, less than 50bp, insertions, deletion as commonly identified by most short reads variant callers. Structural variations which include insertions, deletions larger than 50bp and which are typically identified using dedicated SV callers are not considered.

                              +
                              + +
                              + + +
                              +
                              + +
                              +
                              +
                              +
                              + + + + \ No newline at end of file diff --git a/docs/api/metrics.ratio_transitions_transversions.html b/docs/api/metrics.ratio_transitions_transversions.html new file mode 100644 index 00000000..9c7ca3b9 --- /dev/null +++ b/docs/api/metrics.ratio_transitions_transversions.html @@ -0,0 +1,132 @@ + + + + + + + ratio_transitions_transversions — NPM-sample-qc documentation + + + + + + + + + + + + + + + + + + +
                              + + +
                              + +
                              +
                              +
                              + +
                              +
                              +
                              +
                              + +
                              +

                              ratio_transitions_transversions

                              +
                              +
                              +metrics.ratio_transitions_transversions(mqc, biosample_id)
                              +

                              Description: The ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality variants.

                              +

                              Implementation details: In the NPM-sample-QC reference implementation, calculate the ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality-variants by bcftools stats, (bcftools stats -f PASS … TSTV).

                              +
                              + +
                              + + +
                              +
                              + +
                              +
                              +
                              +
                              + + + + \ No newline at end of file diff --git a/docs/api/metrics.yield_bp_q30.html b/docs/api/metrics.yield_bp_q30.html index e728b0e6..b4157113 100644 --- a/docs/api/metrics.yield_bp_q30.html +++ b/docs/api/metrics.yield_bp_q30.html @@ -20,7 +20,7 @@ - + @@ -41,6 +41,9 @@

                              Metric definitions

                              • Functions
                                • @@ -94,7 +101,7 @@

                                yield_bp_q30 - +
                                diff --git a/docs/genindex.html b/docs/genindex.html index 12cea1f0..5742993d 100644 --- a/docs/genindex.html +++ b/docs/genindex.html @@ -72,12 +72,33 @@

                                Index

                                | I | M | P + | R | Y

                                C

                                +
                                +

                                R

                                + + + +
                                +

                                Y

                                - + + + + + + + + + + - + - + - + - + - + - + - + - + + + + + + + + + + + + + @@ -123,7 +151,7 @@

                                Functions - +
                                diff --git a/docs/objects.inv b/docs/objects.inv index 15d921a657b73ae4f399374b2603123c61863445..b8969c8a6c0beb3d287b8c04a73097446965acc9 100644 GIT binary patch delta 658 zcmV;D0&V@m1c3&Sdw;E$OK#gR5Qg_W#Q^PAZGxhk9H2lKDGc-g1S7E)QHf+o+Ng5+ zQl=lW#34l`J9!@8F!4|SqnY72UJ>Q!^-I6MH;;%gt`Us~69pF*bk3>CS=Yju#0 zHoW6bD0%AtbCC7;0UqxNv&ukRqJ*^EtYh6uF_@LdJZ3D40Dp|Kmexu-)(gqTZfcn0 zDIC(Wgol$;E8aM@uJQksqd3(k=xEOp-?N6YgHu-Z+@I&j4cn&<9<7gHIg@4FXafSC z{Czp(q%;QGrsQ%(W1~xyoMB`HjCYr-7iDb`D+()>CJrhUW16&1eJDfM?s^zYAHV8%yeG|BF#*hGAp zg=mW8H&%2MzTZMTM^d|Na?E_^g?W@D%>ZM^T>}6hPCY z%rCI2M8JUc=q5*bpQYW delta 465 zcmV;?0WSW52Eqi8dw->t%}&EG41n)>3QKS;KSEsg07x8$Ch-7KmY6M~X+x4tl&7a9 zjCE_Aw&Pa$=d)|qb`1unr3c$AOs#e$!W{^!Z%vfnY=bGUqNccpf*s`2)!uViS3wwE ziJI}xx0z3nR53E=(&MY+TneYmbXy1>2uLhgg&3itZQK7T_eeTH}=6c@Wt``Tce z&mNuF9%T;i5j1u(@5OL38_0FY`n zy6>ro^l^Zsij7|YG8BC;AgyD$+mH=&ena3~%Ce(IvC}gN12M~uUJgDOQ8<^fN%J=z zBQ>xSayJeG&($Yk4o%l_8X;V@v>SLUKrcn`VYwx z3*uxsJpFDQ&)WZkM0DE}2Ss#P#vhEBM^8KAwraw*9q|)ykHX3AsbiEypVz2gbszmV He6j{F5?1Nz diff --git a/docs/searchindex.js b/docs/searchindex.js index 699252f1..0bedd451 100644 --- a/docs/searchindex.js +++ b/docs/searchindex.js @@ -1 +1 @@ -Search.setIndex({"docnames": ["api/metrics", "api/metrics.cross_contamination_rate", "api/metrics.insert_size_std_deviation", "api/metrics.mad_autosome_coverage", "api/metrics.mean_autosome_coverage", "api/metrics.mean_insert_size", "api/metrics.pct_autosomes_15x", "api/metrics.pct_reads_mapped", "api/metrics.pct_reads_properly_paired", "api/metrics.yield_bp_q30", "index", "metrics"], "filenames": ["api/metrics.rst", "api/metrics.cross_contamination_rate.rst", "api/metrics.insert_size_std_deviation.rst", "api/metrics.mad_autosome_coverage.rst", "api/metrics.mean_autosome_coverage.rst", "api/metrics.mean_insert_size.rst", "api/metrics.pct_autosomes_15x.rst", "api/metrics.pct_reads_mapped.rst", "api/metrics.pct_reads_properly_paired.rst", "api/metrics.yield_bp_q30.rst", "index.rst", "metrics.rst"], "titles": ["metrics package", "cross_contamination_rate", "insert_size_std_deviation", "mad_autosome_coverage", "mean_autosome_coverage", "mean_insert_size", "pct_autosomes_15x", "pct_reads_mapped", "pct_reads_properly_paired", "yield_bp_q30", "NPM-sample-qc", "Functions"], "terms": {"cross_contamination_r": 0, "mqc": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "biosample_id": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "descript": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "estim": [0, 1], "inter": [0, 1], "sampl": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "contamin": [0, 1], "rate": [0, 1], "short": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10], "pair": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "end": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "sequenc": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10], "high": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "qualiti": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "non": [0, 1, 3, 4, 6, 10], "duplic": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "read": [0, 1, 2, 3, 4, 5, 6, 7, 8, 9], "primari": [0, 1, 2, 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                                  diff --git a/docs/index.html b/docs/index.html index 213f001d..366abf25 100644 --- a/docs/index.html +++ b/docs/index.html @@ -75,9 +75,9 @@

                                  Requirements

                                • Install Nextflow

                                -

                                v23.08.1-edge or higher

                                +

                                v23.10.4 or higher

                                # Download the executable package
-wget -qO- https://github.com/nextflow-io/nextflow/releases/download/v23.08.1-edge/nextflow-23.08.1-edge-all | bash
+wget -qO- https://github.com/nextflow-io/nextflow/releases/download/v23.10.4/nextflow-23.10.4-all | bash
 # Make the binary executable on your system
 chmod -x nextflow
 # Optionally, move the nextflow file to a directory accessible by your $PATH variable
@@ -103,7 +103,7 @@ 
                                Quick start
                                diff output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json output/results/multiqc/NA12878-chr14-AKT1.metrics.json
+
                                diff output_certified/results/metrics/NA12878-chr14-AKT1.metrics.json output/results/metrics/NA12878-chr14-AKT1.metrics.json
 
                                
 
                                
 
                                Please refer to the workflow help for more information on its usage and access to additional options:
                                
@@ -117,7 +117,7 @@ 
                                Understanding the workflow
                                
 
                                The workflow requires the following resources given in the conf/resources.config
                                -

                                If keep_workdir has been specified, the contents of the Nextflow work directory (work-dir) will also be preserved.

                                +

                                If cleanup = true in the nextflow.config is commented out, the contents of the Nextflow work directory (work-dir) will also be preserved.

                                Docker image

                                @@ -182,11 +184,9 @@

                                Workflow logic

                                In a nutshell, this workflow generates QC metrics from single-sample WGS results in three stages: metrics calculation, parsing of intermediate outputs and generation of a final report. This makes it possible to take full advantage of the parallelisation capabilities of Nextflow, allows users to leverage third-party tools or add custom scripts, and enables auto-documentation of metrics from code comments.

                                Metrics calculation

                                -

                                The current workflow combines widely-used third-party tools (samtools, picard, mosdepth) and custom scripts. Full details on which processes are run/when can be found in the actual workflow definition (main.nf). We also provide an example dag for a more visual representation (tests/NA12878_1000genomes-dragen-3.7.6/dag.pdf).

                                +

                                The current workflow combines widely-used third-party tools (samtools, picard, bcftools, verifybamid2) and custom scripts. Full details on which processes are run/when can be found in the actual workflow definition (main.nf). We also provide an example dag for a more visual representation (tests/NA12878_1000genomes-dragen-3.7.6/dag.pdf).

                                Metrics parsing

                                -

                                Next, output files from each individual tool are parsed and combined into a single json file. This is done by calling bin/multiqc_plugins/multiqc_npm/, a MultiQC plugin that extends the base tool to support additional files.

                                -

                                Metrics reporting

                                -

                                Finally, the contents of the MultiQC json are formatted into a final metrics report, also in json format. The reporting logic lives in the bin/compile_metrics.py script, and whilst its contents are simple, it enables automatic documentation of metric definitions from code comments (see the Metric definitions section).

                                +

                                Next, output files from each individual tool are parsed and combined into a single json file.

                                Metric definitions

                                diff --git a/docs/metrics.html b/docs/metrics.html index 296429e2..192e4edd 100644 --- a/docs/metrics.html +++ b/docs/metrics.html @@ -20,7 +20,7 @@ - + @@ -42,6 +42,9 @@

                                Metric definitions

                                cross_contamination_rate(mqc, biosample_id)

                                count_deletions(mqc, biosample_id)

                                Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

                                count_insertions(mqc, biosample_id)

                                Description: The number of variant type indels only insertions in VCF, only in autosomal regions, high quality variants.

                                count_snvs(mqc, biosample_id)

                                Description: The number of variant type SNVs in VCF, only in autosomal regions, high quality variants.

                                cross_contamination_rate(mqc, biosample_id)

                                Description: Estimation of inter-sample contamination rate of short paired-end sequencing high quality, non duplicated reads, primary alignments, mapped on GRCh38 assembly.

                                insert_size_std_deviation(mqc, biosample_id)

                                insert_size_std_deviation(mqc, biosample_id)

                                Description: The insert size standard deviation of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly.

                                mad_autosome_coverage(mqc, biosample_id)

                                mad_autosome_coverage(mqc, biosample_id)

                                Description: The median absolute deviation of sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly.

                                mean_autosome_coverage(mqc, biosample_id)

                                mean_autosome_coverage(mqc, biosample_id)

                                Description: The mean sequencing coverage derived from short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly.

                                mean_insert_size(mqc, biosample_id)

                                mean_insert_size(mqc, biosample_id)

                                Description: The mean insert size of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly.

                                pct_autosomes_15x(mqc, biosample_id)

                                pct_autosomes_15x(mqc, biosample_id)

                                Description: The percentage of bases attaining at least 15X sequencing coverage in short paired-end sequencing high quality, non duplicated reads, primary alignments, achieving a base quality of 20 or greater and mapping quality of 20 or greater, in autosomes non gap regions of GRCh38 assembly.

                                pct_reads_mapped(mqc, biosample_id)

                                pct_reads_mapped(mqc, biosample_id)

                                Description: The percentage of short paired-end sequencing high quality reads, primary alignments, mapped on GRCh38 assembly.

                                pct_reads_properly_paired(mqc, biosample_id)

                                pct_reads_properly_paired(mqc, biosample_id)

                                Description: The percentage of short paired-end sequencing high quality, properly paired reads, primary alignments, mapped on GRCh38 assembly.

                                yield_bp_q30(mqc, biosample_id)

                                ratio_heterozygous_homzygous_indel(mqc, ...)

                                Description: The ratio of heterozygous and homozygous variant type indels in VCF, only in autosomal regions, high quality variants.

                                ratio_heterozygous_homzygous_snv(mqc, ...)

                                Description: The ratio of heterozygous and homozygous variant type SNVs in VCF, only in autosomal regions, high quality variants.

                                ratio_insertion_deletion(mqc, biosample_id)

                                Description: The ratio between number of insertion and deletion in VCF, only in autosomal regions, high quality variants.

                                ratio_transitions_transversions(mqc, ...)

                                Description: The ratio of transitions and transversions of bi-allelic SNVs in VCF, only in autosomal regions, high quality variants.

                                yield_bp_q30(mqc, biosample_id)

                                Description: The number of bases in short paired-end sequencing high quality reads, primary alignments, achieving a base quality score of 30 or greater (Phred scale).

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ends.. chr14:101769349-106795748 + +bcftools view -r chr14:101769349-106795748 NA12878.hard.filtered.vcf.gz >NA12878-chr14-AKT1.vcf +bcftools view NA12878-chr14-AKT1.vcf -Oz -o NA12878-chr14-AKT1.vcf.gz + +tabix -p vcf NA12878-chr14-AKT1.vcf.gz From 617caa714ce957949903d21266d20d88d9b167d7 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Mon, 27 May 2024 06:43:39 +0000 Subject: [PATCH 041/132] update metrics qc IDs --- bin/compile_metrics.py | 8 ++-- bin/count_variants.py | 96 ++++-------------------------------------- bin/metrics/metrics.py | 30 ++++++++++--- 3 files changed, 37 insertions(+), 97 deletions(-) diff --git a/bin/compile_metrics.py b/bin/compile_metrics.py index 460c2740..fdc7d5e4 100755 --- a/bin/compile_metrics.py +++ b/bin/compile_metrics.py @@ -55,10 +55,10 @@ def calculate_metrics(mqc): "insert_size_std_deviation", "cross_contamination_rate", # variant calling - "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", - "pass_indel_het_hom", - "pass_del", "pass_ins", - "pass_snp_ts_tv" + "count_snvs", "ratio_heterozygous_homzygous_snv", + "ratio_heterozygous_homzygous_indel", + "count_deletions", "count_insertions", "ratio_insertion_deletion", + "ratio_transitions_transversions" ] result = dict() logging.info("Calculating %d metrics..." % len(metrics_list)) diff --git a/bin/count_variants.py b/bin/count_variants.py index 8715dbd8..ac3aa29f 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -66,16 +66,15 @@ def count_variants(input_vcf, scratch_dir): # initialise results dict r = dict() metrics_list = [ - "all_snps", "all_het_snps", "all_homalt_snps", "all_snp_het_hom", + "all_snps", "pass_snps", "pass_het_snps", "pass_homalt_snps", "pass_snp_het_hom", - "all_indels", "all_het_indels", "all_homalt_indels", "all_indel_het_hom", + "all_indels", "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", - "all_del", "all_ins", "pass_del", "pass_ins", + "pass_del", "pass_ins", "pass_ins_del", "all_mnps", "pass_mnps", - "all_complex_indels", "all_complex_ins", "all_complex_del", "pass_complex_indels", "pass_complex_ins", "pass_complex_del", - "all_multiallelic_sites", "all_multiallelic_sites", - "all_snp_ts_tv", "pass_snp_ts_tv" + "pass_multiallelic_sites", + "pass_snp_ts_tv" ] for m in metrics_list: r[m] = 0 @@ -98,22 +97,6 @@ def count_variants(input_vcf, scratch_dir): p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["all_snps"] = int(p.stdout.read()) - logging.info("Counting all_het_snps...") - cmd = "bcftools view -H -v snps -g het %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_het_snps"] = int(p.stdout.read()) - - logging.info("Counting all_homalt_snps...") - cmd = "bcftools view -H -v snps -g hom %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_homalt_snps"] = int(p.stdout.read()) - - logging.info("Calculating all_snp_het_hom...") - snp_het_hom = np.divide(r["all_het_snps"], r["all_homalt_snps"]) - r["all_snp_het_hom"] = np.round(snp_het_hom, 2) - logging.info("Counting pass_snps...") cmd = "bcftools view -H -v snps -f PASS %s | wc -l" % input_vcf logging.debug("CMD: %s" % cmd) @@ -142,22 +125,6 @@ def count_variants(input_vcf, scratch_dir): p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["all_indels"] = int(p.stdout.read()) - logging.info("Counting all_het_indels...") - cmd = "bcftools view -H -v indels -g het %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_het_indels"] = int(p.stdout.read()) - - logging.info("Counting all_homalt_indels...") - cmd = "bcftools view -H -v indels -g hom %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_homalt_indels"] = int(p.stdout.read()) - - logging.info("Calculating all_indel_het_hom...") - indel_het_hom = np.divide(r["all_het_indels"], r["all_homalt_indels"]) - r["all_indel_het_hom"] = np.round(indel_het_hom, 2) - logging.info("Counting pass_indels...") cmd = "bcftools view -H -v indels -f PASS %s | wc -l" % input_vcf logging.debug("CMD: %s" % cmd) @@ -180,28 +147,6 @@ def count_variants(input_vcf, scratch_dir): indel_het_hom = np.divide(r["pass_het_indels"], r["pass_homalt_indels"]) r["pass_indel_het_hom"] = np.round(indel_het_hom, 2) - logging.info("Counting all_del, all_ins...") - cmd = "bcftools view -H -v indels %s" % input_vcf - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF), label as INS - cmd += " | awk '{ if( length($5) > length($4) ) { print \"INS\" }" - # if length(ALT) < length(REF), label as DEL - cmd += " else if ( length($5) < length($4) ) { print \"DEL\" }" - # label everything else as UNK and report its location - cmd += " else { print \"UNK\" } }'" - # count by label - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "all_%s" % l[1].lower() - v = int(l[0]) - r[k] = v logging.info("Counting pass_del, pass_ins...") cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf @@ -226,6 +171,10 @@ def count_variants(input_vcf, scratch_dir): v = int(l[0]) r[k] = v + logging.info("Calculating pass_ins_del...") + ins_del = np.divide(r["pass_ins"], r["pass_del"]) + r["pass_ins_del"] = np.round(ins_del, 2) + logging.info("Counting all_mnps...") cmd = "bcftools view -H -v mnps %s | wc -l" % input_vcf logging.debug("CMD: %s" % cmd) @@ -238,26 +187,6 @@ def count_variants(input_vcf, scratch_dir): p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_mnps"] = int(p.stdout.read()) - logging.info("Counting all_complex_indels...") - cmd = "bcftools view -H -v indels %s" % input_vcf - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF) and length(REF) > 1, label as COMPLEX_INS - cmd += " | awk '{ if( length($5) > length($4) && length($4) > 1 ) { print \"COMPLEX_INS\" }" - # if length(ALT) < length(REF) and length(ALT) > 1, label as COMPLEX_DEL - cmd += " else if ( length($5) < length($4) && length($5) > 1 ) { print \"COMPLEX_DEL\" } }'" - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "all_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - r["all_complex_indels"] = r["all_complex_ins"] + r["all_complex_del"] logging.info("Counting pass_complex_indels...") cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf @@ -292,13 +221,6 @@ def count_variants(input_vcf, scratch_dir): p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_multiallelic_sites"] = int(p.stdout.read()) - logging.info("Calculating all_snp_ts_tv...") - cmd = "cat %s/all.bcftools_stats | grep '^TSTV'" % scratch_dir - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - l = p.stdout.read().decode("utf-8").strip().split("\t") - r["all_snp_ts_tv"] = np.round(float(l[4]), 2) - logging.info("Calculating pass_snp_ts_tv...") cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir logging.debug("CMD: %s" % cmd) diff --git a/bin/metrics/metrics.py b/bin/metrics/metrics.py index c123dfc1..cafbe7b7 100644 --- a/bin/metrics/metrics.py +++ b/bin/metrics/metrics.py @@ -158,7 +158,7 @@ def cross_contamination_rate(mqc, biosample_id): return k, v -def pass_snps(mqc, biosample_id): +def count_snvs(mqc, biosample_id): """ The number of PASS SNPs. @@ -209,7 +209,7 @@ def pass_homalt_snps(mqc, biosample_id): return k, v -def pass_snp_het_hom(mqc, biosample_id): +def ratio_heterozygous_homzygous_snv(mqc, biosample_id): """ The het/hom ratio for PASS SNPs. @@ -226,7 +226,7 @@ def pass_snp_het_hom(mqc, biosample_id): return k, v -def pass_indel_het_hom(mqc, biosample_id): +def ratio_heterozygous_homzygous_indel(mqc, biosample_id): """ The het/hom ratio for PASS INDELs. @@ -243,7 +243,7 @@ def pass_indel_het_hom(mqc, biosample_id): return k, v -def pass_del(mqc, biosample_id): +def count_deletions(mqc, biosample_id): """ The number of PASS deletions. @@ -260,7 +260,7 @@ def pass_del(mqc, biosample_id): return k, v -def pass_ins(mqc, biosample_id): +def count_insertions(mqc, biosample_id): """ The number of PASS insertions. @@ -277,7 +277,25 @@ def pass_ins(mqc, biosample_id): return k, v -def pass_snp_ts_tv(mqc, biosample_id): +def ratio_insertion_deletion(mqc, biosample_id): + """ + The ins/del ratio for PASS INDELs. + + Source: count_variants.py (bcftools view) + """ + k = inspect.currentframe().f_code.co_name + + try: + d = mqc["multiqc_npm_count_variants"][biosample_id + ".variant"] + v = d["pass_ins_del"] + v = np.round(v, DECIMALS) + except KeyError: + v = "NA" + + return k, v + + +def ratio_transitions_transversions(mqc, biosample_id): """ The transition to transversion ratio of passing bi-allelic SNPs. 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13248182 13254852 +chr22 13254952 13258197 +chr22 13258297 13280858 +chr22 13280958 13285143 +chr22 13285243 14419454 +chr22 14419554 14419894 +chr22 14419994 14420334 +chr22 14420434 14421632 +chr22 14421732 15054318 +chr22 15154318 16279672 +chr22 16302843 16304296 +chr22 16305427 16307048 +chr22 16307605 16310302 +chr22 16310402 16313516 +chr22 16314010 18239129 +chr22 18339129 18433513 +chr22 18483513 18659564 +chr22 18709564 49973865 +chr22 49975365 50808468 From ade77b02301348a8c99229535ea3f8a0605404f7 Mon Sep 17 00:00:00 2001 From: justinjj24 Date: Wed, 29 May 2024 02:48:08 +0000 Subject: [PATCH 043/132] regenerate autosomes_non_gap_regions.bed --- resources/autosomes_non_gap_regions.bed | 4063 +++-------------------- 1 file changed, 485 insertions(+), 3578 deletions(-) diff --git a/resources/autosomes_non_gap_regions.bed b/resources/autosomes_non_gap_regions.bed index 09ecb69d..660b7668 100644 --- a/resources/autosomes_non_gap_regions.bed +++ b/resources/autosomes_non_gap_regions.bed @@ -1,3578 +1,485 @@ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - NPM-sample-qc/autosomes_non_gap_regions.bed at feature/metrics-fix · c-BIG/NPM-sample-qc · GitHub - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

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From: justinjj24 Date: Wed, 29 May 2024 09:37:09 +0000 Subject: [PATCH 044/132] cleanup the metrics and included bcftools -R autosomes_non_gap_regions.bed --- bin/count_variants.py | 82 ++++-------------- .../NA12878-chr14-AKT1.vcf.gz | Bin 0 -> 609662 bytes .../NA12878-chr14-AKT1.vcf.gz.tbi | Bin 0 -> 3298 bytes 3 files changed, 17 insertions(+), 65 deletions(-) create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.gz create mode 100644 tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.gz.tbi diff --git a/bin/count_variants.py b/bin/count_variants.py index ac3aa29f..bcb0e263 100755 --- a/bin/count_variants.py +++ b/bin/count_variants.py @@ -61,7 +61,7 @@ def setup(input_vcf, scratch_dir): return variants_vcf -def count_variants(input_vcf, scratch_dir): +def count_variants(input_vcf, scratch_dir, regions): # initialise results dict r = dict() @@ -71,9 +71,6 @@ def count_variants(input_vcf, scratch_dir): "all_indels", "pass_indels", "pass_het_indels", "pass_homalt_indels", "pass_indel_het_hom", "pass_del", "pass_ins", "pass_ins_del", - "all_mnps", "pass_mnps", - "pass_complex_indels", "pass_complex_ins", "pass_complex_del", - "pass_multiallelic_sites", "pass_snp_ts_tv" ] for m in metrics_list: @@ -81,36 +78,39 @@ def count_variants(input_vcf, scratch_dir): # run bcftools stats - used to obtain ts/tv and sanity checks logging.info("Running bcftools stats for all variants...") - cmd = "bcftools stats %s > %s/all.bcftools_stats" % (input_vcf, scratch_dir) + cmd = "tabix -p vcf %s" % input_vcf + cmd1 = "bcftools stats -R %s %s > %s/all.bcftools_stats" % (regions, input_vcf, scratch_dir) logging.debug("CMD: %s" % cmd) + logging.debug("CMD: %s" % cmd1) p = subprocess.run(cmd, shell=True) + p = subprocess.run(cmd1, shell=True) logging.info("Running bcftools stats for PASS variants...") - cmd = "bcftools stats -f PASS %s > %s/pass.bcftools_stats" % (input_vcf, scratch_dir) + cmd = "bcftools stats -f PASS -R %s %s > %s/pass.bcftools_stats" % (regions, input_vcf, scratch_dir) logging.debug("CMD: %s" % cmd) p = subprocess.run(cmd, shell=True) # calculate metrics logging.info("Counting all_snps...") - cmd = "bcftools view -H -v snps %s | wc -l" % input_vcf + cmd = "bcftools view -H -v snps -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["all_snps"] = int(p.stdout.read()) logging.info("Counting pass_snps...") - cmd = "bcftools view -H -v snps -f PASS %s | wc -l" % input_vcf + cmd = "bcftools view -H -v snps -f PASS -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_snps"] = int(p.stdout.read()) logging.info("Counting pass_het_snps...") - cmd = "bcftools view -H -v snps -f PASS -g het %s | wc -l" % input_vcf + cmd = "bcftools view -H -v snps -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_het_snps"] = int(p.stdout.read()) logging.info("Counting pass_homalt_snps...") - cmd = "bcftools view -H -v snps -f PASS -g hom %s | wc -l" % input_vcf + cmd = "bcftools view -H -v snps -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_homalt_snps"] = int(p.stdout.read()) @@ -120,25 +120,25 @@ def count_variants(input_vcf, scratch_dir): r["pass_snp_het_hom"] = np.round(snp_het_hom, 2) logging.info("Counting all_indels...") - cmd = "bcftools view -H -v indels %s | wc -l" % input_vcf + cmd = "bcftools view -H -v indels -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["all_indels"] = int(p.stdout.read()) logging.info("Counting pass_indels...") - cmd = "bcftools view -H -v indels -f PASS %s | wc -l" % input_vcf + cmd = "bcftools view -H -v indels -f PASS -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_indels"] = int(p.stdout.read()) logging.info("Counting pass_het_indels...") - cmd = "bcftools view -H -v indels -f PASS -g het %s | wc -l" % input_vcf + cmd = "bcftools view -H -v indels -f PASS -g het -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_het_indels"] = int(p.stdout.read()) logging.info("Counting pass_homalt_indels...") - cmd = "bcftools view -H -v indels -f PASS -g hom %s | wc -l" % input_vcf + cmd = "bcftools view -H -v indels -f PASS -g hom -R %s %s | wc -l" % (regions, input_vcf) logging.debug("CMD: %s" % cmd) p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) r["pass_homalt_indels"] = int(p.stdout.read()) @@ -149,7 +149,7 @@ def count_variants(input_vcf, scratch_dir): logging.info("Counting pass_del, pass_ins...") - cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf + cmd = "bcftools view -H -v indels -f PASS -R %s %s" % (regions, input_vcf) # filter dragen annotation of variant records cmd += " | sed 's/,//g'" # exclude multi-allelic sites @@ -175,52 +175,6 @@ def count_variants(input_vcf, scratch_dir): ins_del = np.divide(r["pass_ins"], r["pass_del"]) r["pass_ins_del"] = np.round(ins_del, 2) - logging.info("Counting all_mnps...") - cmd = "bcftools view -H -v mnps %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_mnps"] = int(p.stdout.read()) - - logging.info("Counting pass_mnps...") - cmd = "bcftools view -H -v mnps -f PASS %s | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_mnps"] = int(p.stdout.read()) - - - logging.info("Counting pass_complex_indels...") - cmd = "bcftools view -H -v indels -f PASS %s" % input_vcf - # filter dragen annotation of variant records - cmd += " | sed 's/,//g'" - # exclude multi-allelic sites - cmd += " | awk '$4 !~ /,/ && $5 !~ /,/'" - # if length(ALT) > length(REF) and length(REF) > 1, label as COMPLEX_INS - cmd += " | awk '{ if( length($5) > length($4) && length($4) > 1 ) { print \"COMPLEX_INS\" }" - # if length(ALT) < length(REF) and length(ALT) > 1, label as COMPLEX_DEL - cmd += " else if ( length($5) < length($4) && length($5) > 1 ) { print \"COMPLEX_DEL\" } }'" - cmd += " | sort | uniq -c" - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - x = p.stdout.read() - for l in x.splitlines(): - l = l.decode("utf-8").strip().split(" ") - k = "pass_%s" % l[1].lower() - v = int(l[0]) - r[k] = v - r["pass_complex_indels"] = r["pass_complex_ins"] + r["pass_complex_del"] - - logging.info("Calculating all_multiallelic_sites...") - cmd = "bcftools view -H %s | sed 's/,//g' | awk '$5 ~ /,/' | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["all_multiallelic_sites"] = int(p.stdout.read()) - - logging.info("Calculating pass_multiallelic_sites...") - cmd = "bcftools view -H -f PASS %s | sed 's/,//g' | awk '$5 ~ /,/' | wc -l" % input_vcf - logging.debug("CMD: %s" % cmd) - p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True) - r["pass_multiallelic_sites"] = int(p.stdout.read()) - logging.info("Calculating pass_snp_ts_tv...") cmd = "cat %s/pass.bcftools_stats | grep '^TSTV'" % scratch_dir logging.debug("CMD: %s" % cmd) @@ -245,9 +199,6 @@ def count_variants(input_vcf, scratch_dir): elif "indels" in k and v != r["all_indels"]: logging.error("Mismatch in INDEL counts: bcftools stats=%d, count_variants=%d" % (v, r["all_indels"])) error_count += 1 - elif "mnps" in k and v != r["all_mnps"]: - logging.error("Mismatch in MNP counts: bcftools stats=%d, count_variants=%d" % (v, r["all_mnps"])) - error_count += 1 if error_count > 0: sys.exit("Sanity checks failed, aborting execution") @@ -266,6 +217,7 @@ def done(scratch_dir): # clean up logging.info("Cleaning up...") os.remove("%s/variants.vcf.gz" % scratch_dir) + os.remove("%s/variants.vcf.gz.tbi" % scratch_dir) os.remove("%s/all.bcftools_stats" % scratch_dir) os.remove("%s/pass.bcftools_stats" % scratch_dir) if len(os.listdir(scratch_dir)) == 0: @@ -281,7 +233,7 @@ def done(scratch_dir): variants_vcf = setup(args.input_vcf, args.scratch_dir) - vcf_metrics = count_variants(variants_vcf, args.scratch_dir) + vcf_metrics = count_variants(variants_vcf, args.scratch_dir, args.regions) save_output(vcf_metrics, args.output_json) done(args.scratch_dir) diff --git a/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.gz b/tests/NA12878-chr14-AKT1_1000genomes-dragen-3.7.6/NA12878-chr14-AKT1.vcf.gz new file mode 100644 index 0000000000000000000000000000000000000000..82cf8fa36919e9efdb93b22140182ed81c03afdb GIT binary patch literal 609662 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