From b51c5ff9bf83f51525c356795a048213ec4bd1ef Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Tue, 7 Jul 2020 19:12:20 -0700 Subject: [PATCH 01/12] BUG: match feature table and ordination This would otherwise lead to situations where mismatching categories would be displayed in the ordination and phylogeny. In principle users should use the rarefied table that was used for generating the ordination i.e. samples should have a 1:1 match. Fixes #204 --- empress/core.py | 2 +- empress/tools.py | 16 +++++++++++++ tests/python/test_tools.py | 48 +++++++++++++++++++++++++++++++++++++- 3 files changed, 64 insertions(+), 2 deletions(-) diff --git a/empress/core.py b/empress/core.py index fa96c4d81..51a933cd6 100644 --- a/empress/core.py +++ b/empress/core.py @@ -136,7 +136,7 @@ def _validate_and_match_data(self, ignore_missing_samples, # table for the rest of this visualizer. self.table, self.samples, self.tip_md, self.int_md = match_inputs( self.tree, self.table.T, self.samples, self.features, - ignore_missing_samples, filter_missing_features + self.ordination, ignore_missing_samples, filter_missing_features ) # remove unobserved features from the phylogeny if filter_unobserved_features_from_phylogeny: diff --git a/empress/tools.py b/empress/tools.py index d916db956..33cb1769d 100644 --- a/empress/tools.py +++ b/empress/tools.py @@ -59,6 +59,7 @@ def match_inputs( table, sample_metadata, feature_metadata=None, + ordination=None, ignore_missing_samples=False, filter_missing_features=False ): @@ -87,6 +88,8 @@ def match_inputs( IDs and the columns should describe different feature metadata fields' names. (Feature IDs here can describe tips or internal nodes in the tree.) + ordination: skbio.OrdinationResults, optional + The ordination to display in a tandem plot. ignore_missing_samples: bool If True, pads missing samples (i.e. samples in the table but not the metadata) with placeholder metadata. If False, raises a @@ -136,6 +139,8 @@ def match_inputs( metadata, AND ignore_missing_samples is False. 5. The feature metadata was passed, but no features present in it are also present as tips or internal nodes in the tree. + 6. The ordination AND feature table don't have exactly the same + samples. References ---------- @@ -271,4 +276,15 @@ def match_inputs( "either as tips or as internal nodes." ) + if ordination is not None: + # tandem plots require a 1-1 match between feature table and ordination + mismatched = set(ordination.samples.index) ^ set(ff_table.columns) + + if mismatched: + raise DataMatchingError( + "The feature table does not have exactly the same samples as " + "the ordination. These are the problematic sample identifiers:" + " %s" % (', '.join(sorted(mismatched))) + ) + return ff_table, sf_sample_metadata, tip_metadata, int_metadata diff --git a/tests/python/test_tools.py b/tests/python/test_tools.py index 1b9263d3e..23201a2c8 100644 --- a/tests/python/test_tools.py +++ b/tests/python/test_tools.py @@ -8,7 +8,7 @@ import unittest import pandas as pd from pandas.testing import assert_frame_equal -from skbio import TreeNode +from skbio import TreeNode, OrdinationResults from empress import Tree, tools from empress.taxonomy_utils import split_taxonomy from bp import parse_newick, from_skbio_treenode @@ -73,6 +73,25 @@ def setUp(self): "Level 7", "Confidence" ] + eigvals = pd.Series([0.50, 0.25, 0.25], + index=['PC1', 'PC2', 'PC3']) + samples = [[0.1, 0.2, 0.3], + [0.2, 0.3, 0.4], + [0.3, 0.4, 0.5], + [0.4, 0.5, 0.6]] + proportion_explained = pd.Series([15.5, 12.2, 8.8], + index=['PC1', 'PC2', 'PC3']) + samples_df = pd.DataFrame(samples, + index=['Sample1', 'Sample2', 'Sample3', + 'Sample4'], + columns=['PC1', 'PC2', 'PC3']) + self.ordination = OrdinationResults( + 'PCoA', + 'Principal Coordinate Analysis', + eigvals, + samples_df, + proportion_explained=proportion_explained) + def test_fill_missing_node_names(self): t = Tree.from_tree(self.tree) tools.fill_missing_node_names(t) @@ -91,6 +110,20 @@ def test_match_inputs_nothing_dropped(self): self.assertIsNone(t_md) self.assertIsNone(i_md) + def test_match_inputs_nothing_dropped_with_ordination(self): + # everything is the same since the ordination has a 1:1 match to the + # feature table + filtered_table, filtered_sample_md, t_md, i_md = tools.match_inputs( + self.bp_tree, self.table, self.sample_metadata, + ordination=self.ordination + ) + + assert_frame_equal(filtered_table, self.table) + assert_frame_equal(filtered_sample_md, self.sample_metadata) + # We didn't pass in any feature metadata, so we shouldn't get any out + self.assertIsNone(t_md) + self.assertIsNone(i_md) + def test_match_inputs_only_1_feature_in_table(self): # This is technically allowed (so long as this 1 feature is a tree tip) tiny_table = self.table.loc[["a"]] @@ -381,6 +414,19 @@ def test_match_inputs_feature_metadata_only_internal_node_metadata(self): self.assertListEqual(list(t_fm.columns), self.exp_split_fm_cols) self.assertListEqual(list(i_fm.columns), self.exp_split_fm_cols) + def test_failed_match_to_ordination(self): + self.ordination.samples.index = pd.Index(['Sample1', 'Zample2', + 'Sample3', 'Sample4']) + + with self.assertRaisesRegex( + tools.DataMatchingError, + "The feature table does not have exactly the same samples as the " + "ordination. These are the problematic sample identifiers: Sample2" + ", Zample2" + ): + tools.match_inputs(self.bp_tree, self.table, self.sample_metadata, + ordination=self.ordination) + if __name__ == "__main__": unittest.main() From ba68daf69e842086a871d8f18c5eec272685b928 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Wed, 8 Jul 2020 08:36:03 -0700 Subject: [PATCH 02/12] Add vim files to gitignore and update table --- .gitignore | 5 +++++ docs/moving-pictures/table.qza | Bin 53704 -> 41773 bytes 2 files changed, 5 insertions(+) diff --git a/.gitignore b/.gitignore index 21e7bafb4..346847d6f 100644 --- a/.gitignore +++ b/.gitignore @@ -142,3 +142,8 @@ dmypy.json # dev scripts/data inf/ create-plt.bash + +# vi swap and temp files +*.swp +*.swo +*~ diff --git a/docs/moving-pictures/table.qza b/docs/moving-pictures/table.qza index c26b61643503afdefb3ccee2abdbf83644d057ca..ae6b1cde7ff933d3f2e024628f9fd262fae8750b 100644 GIT binary patch literal 41773 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empress/plugin_setup.py | 5 +++ empress/tools.py | 53 ++++++++++++++++++-------- tests/python/test_tools.py | 76 +++++++++++++++++++++++++++++++++++--- 4 files changed, 121 insertions(+), 21 deletions(-) diff --git a/README.md b/README.md index fafd7f5ec..78590c8f9 100644 --- a/README.md +++ b/README.md @@ -58,6 +58,14 @@ This generates a visualization of a phylogenetic tree at ### Example 2: Using Empress to visualize a tree in tandem with an ordination +**Note**: In tandem plots we recommend that users carefully consider which +feature table they would like to visualize. These can either be a *filtered +table* (rarefied or without low abundance features), or a *raw table*. If you +select a *filtered table* the tree will include less data than in the *raw +dataset*. If you select the *raw table*, you might find that some nodes in the +tree won't be represented by any of the samples in the ordination (if the +ordination was made using a *filtered table*). + ```bash qiime empress plot \ --i-tree docs/moving-pictures/rooted-tree.qza \ diff --git a/empress/plugin_setup.py b/empress/plugin_setup.py index fc57cd0aa..3f9faa566 100644 --- a/empress/plugin_setup.py +++ b/empress/plugin_setup.py @@ -39,6 +39,7 @@ 'sample_metadata': Metadata, 'feature_metadata': Metadata, 'ignore_missing_samples': Bool, + 'filter_extra_samples': Bool, 'filter_missing_features': Bool, 'number_of_features': Int % Range(1, None), 'filter_unobserved_features_from_phylogeny': Bool @@ -78,6 +79,10 @@ 'metadata". Note that this flag will only be applied if at least ' 'one sample is present in both the feature table and the metadata.' ), + 'filter_extra_samples': ( + 'This will supress the error that\'s raised when samples in the ' + 'feature table are not included in the ordination.' + ), 'filter_missing_features': ( 'This will suppress the error raised when the feature table ' 'contains features that are not present as tips in the tree. ' diff --git a/empress/tools.py b/empress/tools.py index 708d0f4a5..bf6ddf763 100644 --- a/empress/tools.py +++ b/empress/tools.py @@ -61,6 +61,7 @@ def match_inputs( sample_metadata, feature_metadata=None, ordination=None, + filter_extra_samples=False, ignore_missing_samples=False, filter_missing_features=False ): @@ -98,6 +99,10 @@ def match_inputs( no samples are shared between the table and metadata, a DataMatchingError is raised regardless.) This is analogous to the ignore_missing_samples flag in Emperor. + filter_extra_samples: bool, optional + If True, ignores samples in the feature table that are not present in + the ordination. If false, raises a DataMatchingerror if such samples + exist. filter_missing_features: bool If True, filters features from the table that aren't present as tips in the tree. If False, raises a DataMatchingError if any such features @@ -151,16 +156,45 @@ def match_inputs( # (Ignore None-named tips in the tree, which will be replaced later on # with "default" names like "EmpressNode0".) tip_names = set(bp_tree_tips(bp_tree)) - tree_and_table_features = table.index.intersection(tip_names) + ff_table = table.copy() + + if ordination is not None: + table_ids = set(ff_table.columns) + ord_ids = set(ordination.samples.index) + + # don't allow for disjoint datasets + if not (table_ids & ord_ids): + raise DataMatchingError( + "No samples in the feature table are present in the " + "ordination" + ) + + if ord_ids.issubset(table_ids): + extra = table_ids - ord_ids + if extra and not filter_extra_samples: + raise DataMatchingError( + "The feature table has more samples than the ordination. " + "These are the problematic sample identifiers: %s. You can" + " override this error by using the " + "--p-filter-extra-samples flag" % + (', '.join(sorted(extra))) + ) + elif extra: + ff_table = ff_table[ord_ids] + ff_table = ff_table.loc[ff_table.sum(axis=1) > 0] + else: + raise DataMatchingError( + "The ordination has more samples than the feature table" + ) + tree_and_table_features = ff_table.index.intersection(tip_names) if len(tree_and_table_features) == 0: # Error condition 1 raise DataMatchingError( "No features in the feature table are present as tips in the tree." ) - ff_table = table.copy() - if len(tree_and_table_features) < len(table.index): + if len(tree_and_table_features) < len(ff_table.index): if filter_missing_features: # Filter table to just features that are also present in the tree. # @@ -169,7 +203,7 @@ def match_inputs( # (and this is going to be the case in most datasets where the # features correspond to tips, since internal nodes aren't # explicitly described in the feature table). - ff_table = table.loc[tree_and_table_features] + ff_table = ff_table.loc[tree_and_table_features] # Report to user about any dropped features from table. dropped_feature_ct = table.shape[0] - ff_table.shape[0] @@ -276,17 +310,6 @@ def match_inputs( "either as tips or as internal nodes." ) - if ordination is not None: - # tandem plots require a 1-1 match between feature table and ordination - mismatched = set(ordination.samples.index) ^ set(ff_table.columns) - - if mismatched: - raise DataMatchingError( - "The feature table does not have exactly the same samples as " - "the ordination. These are the problematic sample identifiers:" - " %s" % (', '.join(sorted(mismatched))) - ) - return ff_table, sf_sample_metadata, tip_metadata, int_metadata diff --git a/tests/python/test_tools.py b/tests/python/test_tools.py index afd0deb77..974c813a3 100644 --- a/tests/python/test_tools.py +++ b/tests/python/test_tools.py @@ -414,19 +414,83 @@ def test_match_inputs_feature_metadata_only_internal_node_metadata(self): self.assertListEqual(list(t_fm.columns), self.exp_split_fm_cols) self.assertListEqual(list(i_fm.columns), self.exp_split_fm_cols) - def test_failed_match_to_ordination(self): - self.ordination.samples.index = pd.Index(['Sample1', 'Zample2', - 'Sample3', 'Sample4']) + def test_disjoint_table_and_ordination(self): + self.ordination.samples.index = pd.Index(['Zample1', 'Zample2', + 'Zample3', 'Zample4']) with self.assertRaisesRegex( tools.DataMatchingError, - "The feature table does not have exactly the same samples as the " - "ordination. These are the problematic sample identifiers: Sample2" - ", Zample2" + "No samples in the feature table are present in the ordination" ): tools.match_inputs(self.bp_tree, self.table, self.sample_metadata, ordination=self.ordination) + def test_ordination_is_superset(self): + self.table = pd.DataFrame( + { + "Sample1": [1, 2, 0, 4], + "Sample2": [8, 7, 0, 5], + "Sample3": [1, 0, 0, 0], + }, + index=["a", "b", "e", "d"] + ) + + with self.assertRaisesRegex( + tools.DataMatchingError, + "The ordination has more samples than the feature table" + ): + tools.match_inputs(self.bp_tree, self.table, self.sample_metadata, + ordination=self.ordination) + + def test_table_is_superset_raises(self): + self.table = pd.DataFrame( + { + "Sample1": [1, 2, 0, 4], + "Sample2": [8, 7, 0, 5], + "Sample3": [1, 0, 0, 0], + "Sample4": [1, 0, 0, 0], + "Sample5": [1, 0, 4, 0], + }, + index=["a", "b", "e", "d"] + ) + + with self.assertRaisesRegex( + tools.DataMatchingError, + "The feature table has more samples than the ordination. These are" + " the problematic sample identifiers: Sample5. You can override " + "this error by using the --p-filter-extra-samples flag" + ): + tools.match_inputs(self.bp_tree, self.table, self.sample_metadata, + ordination=self.ordination) + + def test_table_is_superset_override_raises(self): + self.table = pd.DataFrame( + { + "Sample1": [1, 2, 0, 4], + "Sample2": [8, 7, 0, 5], + "Sample3": [1, 0, 0, 0], + "Sample4": [1, 0, 0, 0], + "Sample5": [1, 0, 4, 0], + }, + index=["a", "b", "e", "d"] + ) + + filtered_table, filtered_sample_md, t_md, i_md = tools.match_inputs( + self.bp_tree, self.table, self.sample_metadata, + ordination=self.ordination, filter_extra_samples=True) + + exp = self.table.loc[['a', 'b', 'd'], + ['Sample1', 'Sample2', 'Sample3', 'Sample4']] + + # guarantee the same sample-wise order + assert_frame_equal(filtered_table[exp.columns], exp) + assert_frame_equal(filtered_sample_md.loc[exp.columns], + self.sample_metadata) + + # We didn't pass in any feature metadata, so we shouldn't get any out + self.assertIsNone(t_md) + self.assertIsNone(i_md) + def test_shifting(self): # helper test function to count number of bits in the number def _count_bits(n): From 478710e81b5baf8007dc518cece5a30352a26728 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 11:23:30 -0700 Subject: [PATCH 04/12] BLD: Fix plugin setup --- empress/_plot.py | 2 ++ 1 file changed, 2 insertions(+) diff --git a/empress/_plot.py b/empress/_plot.py index 1196b1408..81e5dc10b 100644 --- a/empress/_plot.py +++ b/empress/_plot.py @@ -29,6 +29,7 @@ def plot(output_dir: str, tree: NewickFormat, feature_table: pd.DataFrame, sample_metadata: qiime2.Metadata, pcoa: OrdinationResults = None, feature_metadata: qiime2.Metadata = None, ignore_missing_samples: bool = False, + filter_extra_samples: bool = False, filter_missing_features: bool = False, number_of_features: int = 5, filter_unobserved_features_from_phylogeny: bool = True) -> None: @@ -56,6 +57,7 @@ def plot(output_dir: str, tree: NewickFormat, feature_table: pd.DataFrame, sample_metadata=sample_metadata, feature_metadata=feature_metadata, ordination=pcoa, ignore_missing_samples=ignore_missing_samples, + filter_extra_samples=filter_extra_samples, filter_missing_features=filter_missing_features, filter_unobserved_features_from_phylogeny=trim_tree) From 2ec2d6e33cc5c30b6e88b5c606eba16b4f39210a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 11:30:48 -0700 Subject: [PATCH 05/12] TST: Add missing parameter --- empress/core.py | 13 ++++++++++--- 1 file changed, 10 insertions(+), 3 deletions(-) diff --git a/empress/core.py b/empress/core.py index d876bebcc..c68e0a837 100644 --- a/empress/core.py +++ b/empress/core.py @@ -29,8 +29,8 @@ class Empress(): def __init__(self, tree, table, sample_metadata, feature_metadata=None, ordination=None, - ignore_missing_samples=False, filter_missing_features=False, - resource_path=None, + ignore_missing_samples=False, filter_extra_samples=False, + filter_missing_features=False, resource_path=None, filter_unobserved_features_from_phylogeny=True): """Visualize a phylogenetic tree @@ -63,6 +63,10 @@ def __init__(self, tree, table, sample_metadata, out; and if no samples are shared between the table and metadata, a DataMatchingError is raised regardless.) This is analogous to the ignore_missing_samples flag in Emperor. + filter_extra_samples: bool, optional (default False) + If True, ignores samples in the feature table that are not present + in the ordination. If false, raises a DataMatchingerror if such + samples exist. filter_missing_features: bool, optional (default False) If True, filters features from the table that aren't present as tips in the tree. If False, raises a DataMatchingError if any such @@ -110,6 +114,7 @@ def __init__(self, tree, table, sample_metadata, self._validate_and_match_data( ignore_missing_samples, + filter_extra_samples, filter_missing_features, filter_unobserved_features_from_phylogeny ) @@ -128,6 +133,7 @@ def __init__(self, tree, table, sample_metadata, self._emperor = None def _validate_and_match_data(self, ignore_missing_samples, + filter_extra_samples, filter_missing_features, filter_unobserved_features_from_phylogeny): @@ -138,7 +144,8 @@ def _validate_and_match_data(self, ignore_missing_samples, # table for the rest of this visualizer. self.table, self.samples, self.tip_md, self.int_md = match_inputs( self.tree, self.table.T, self.samples, self.features, - self.ordination, ignore_missing_samples, filter_missing_features + self.ordination, ignore_missing_samples, filter_extra_samples, + filter_missing_features ) # remove unobserved features from the phylogeny if filter_unobserved_features_from_phylogeny: From ca353cbeb6c81bcc1b793880a403a80652739104 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:24:47 -0700 Subject: [PATCH 06/12] Improve the flow of the code --- empress/tools.py | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/empress/tools.py b/empress/tools.py index bf6ddf763..bd6a405f4 100644 --- a/empress/tools.py +++ b/empress/tools.py @@ -171,15 +171,15 @@ def match_inputs( if ord_ids.issubset(table_ids): extra = table_ids - ord_ids - if extra and not filter_extra_samples: - raise DataMatchingError( - "The feature table has more samples than the ordination. " - "These are the problematic sample identifiers: %s. You can" - " override this error by using the " - "--p-filter-extra-samples flag" % - (', '.join(sorted(extra))) - ) - elif extra: + if extra: + if not filter_extra_samples: + raise DataMatchingError( + "The feature table has more samples than the " + "ordination. These are the problematic sample " + "identifiers: %s. You can override this error by using" + " the --p-filter-extra-samples flag" % + (', '.join(sorted(extra))) + ) ff_table = ff_table[ord_ids] ff_table = ff_table.loc[ff_table.sum(axis=1) > 0] else: From a6308ea4b94d2d6d5026df9907f6ef09157f964c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:25:05 -0700 Subject: [PATCH 07/12] Apply suggestions from code review Co-authored-by: Marcus Fedarko --- empress/plugin_setup.py | 7 +++++-- empress/tools.py | 6 +++--- 2 files changed, 8 insertions(+), 5 deletions(-) diff --git a/empress/plugin_setup.py b/empress/plugin_setup.py index 3f9faa566..929dc251e 100644 --- a/empress/plugin_setup.py +++ b/empress/plugin_setup.py @@ -80,8 +80,11 @@ 'one sample is present in both the feature table and the metadata.' ), 'filter_extra_samples': ( - 'This will supress the error that\'s raised when samples in the ' - 'feature table are not included in the ordination.' + 'This will suppress the error raised when samples in the feature ' + 'table are not included in the ordination. These samples will be ' + 'will be removed from the visualization if this flag is passed. ' + 'Note that this flag will only be applied if at least one sample ' + 'in the table is also present in the ordination.' ), 'filter_missing_features': ( 'This will suppress the error raised when the feature table ' diff --git a/empress/tools.py b/empress/tools.py index bf6ddf763..405eb4149 100644 --- a/empress/tools.py +++ b/empress/tools.py @@ -101,7 +101,7 @@ def match_inputs( ignore_missing_samples flag in Emperor. filter_extra_samples: bool, optional If True, ignores samples in the feature table that are not present in - the ordination. If false, raises a DataMatchingerror if such samples + the ordination. If False, raises a DataMatchingError if such samples exist. filter_missing_features: bool If True, filters features from the table that aren't present as tips in @@ -176,7 +176,7 @@ def match_inputs( "The feature table has more samples than the ordination. " "These are the problematic sample identifiers: %s. You can" " override this error by using the " - "--p-filter-extra-samples flag" % + "--p-filter-extra-samples flag." % (', '.join(sorted(extra))) ) elif extra: @@ -184,7 +184,7 @@ def match_inputs( ff_table = ff_table.loc[ff_table.sum(axis=1) > 0] else: raise DataMatchingError( - "The ordination has more samples than the feature table" + "The ordination has more samples than the feature table." ) tree_and_table_features = ff_table.index.intersection(tip_names) From 41fc8ae1a41f1af3d0776e512dda4e86db743630 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:28:19 -0700 Subject: [PATCH 08/12] Apply suggestions from code review Co-authored-by: Marcus Fedarko --- empress/core.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/empress/core.py b/empress/core.py index c68e0a837..275de809e 100644 --- a/empress/core.py +++ b/empress/core.py @@ -65,7 +65,7 @@ def __init__(self, tree, table, sample_metadata, ignore_missing_samples flag in Emperor. filter_extra_samples: bool, optional (default False) If True, ignores samples in the feature table that are not present - in the ordination. If false, raises a DataMatchingerror if such + in the ordination. If False, raises a DataMatchingError if such samples exist. filter_missing_features: bool, optional (default False) If True, filters features from the table that aren't present as From 75cfddacb636d41790d9876f105bafdd758e9c99 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:29:23 -0700 Subject: [PATCH 09/12] DOC: Add comment from @fedarko Not sure why this didn't commit before --- README.md | 26 +++++++++++++++++++------- 1 file changed, 19 insertions(+), 7 deletions(-) diff --git a/README.md b/README.md index 78590c8f9..8779286f8 100644 --- a/README.md +++ b/README.md @@ -58,13 +58,25 @@ This generates a visualization of a phylogenetic tree at ### Example 2: Using Empress to visualize a tree in tandem with an ordination -**Note**: In tandem plots we recommend that users carefully consider which -feature table they would like to visualize. These can either be a *filtered -table* (rarefied or without low abundance features), or a *raw table*. If you -select a *filtered table* the tree will include less data than in the *raw -dataset*. If you select the *raw table*, you might find that some nodes in the -tree won't be represented by any of the samples in the ordination (if the -ordination was made using a *filtered table*). +**Note**: When your ordination was created from a subset of your original +dataset (e.g. the feature table was rarefied, or certain low-frequency features +or samples were otherwise filtered out), we recommend that you carefully +consider *which* feature table you would like to visualize in Empress. You can +use either: + +- A *filtered table* that matches the ordination (e.g. with rarefaction done, + and/or with low-abundance features/samples removed), or +- A *raw table* -- that is, the original table before performing + rarefaction/filtering for the ordination. + +There are some pros and cons for either of these choices. If you use a +*filtered table*, then the Empress visualization will include less data than in +the *raw dataset*: this will impact sample presence information, sample +metadata coloring, and other parts of the visualization. If you select the *raw +table*, you might find that some nodes in the tree won't be represented by any +of the samples in the ordination (if the ordination was made using a *filtered +table*). If you'd like to read more about this, there's some informal +discussion in [pull request 237](https://github.com/biocore/empress/pull/237). ```bash qiime empress plot \ From 96d7944efc5ddc416c94a625a8f342a445832072 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:30:36 -0700 Subject: [PATCH 10/12] =?UTF-8?q?DOC:=20Add=20note=20about=20pandas=20?= =?UTF-8?q?=F0=9F=90=BC?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- empress/tools.py | 1 + 1 file changed, 1 insertion(+) diff --git a/empress/tools.py b/empress/tools.py index 842552809..5a3d580e8 100644 --- a/empress/tools.py +++ b/empress/tools.py @@ -181,6 +181,7 @@ def match_inputs( (', '.join(sorted(extra))) ) ff_table = ff_table[ord_ids] + # remove empty features ff_table = ff_table.loc[ff_table.sum(axis=1) > 0] else: raise DataMatchingError( From 30e851492b9682aa50729b5f5d59d89a6f4ba934 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Yoshiki=20V=C3=A1zquez=20Baeza?= Date: Thu, 9 Jul 2020 16:57:57 -0700 Subject: [PATCH 11/12] DOC: Document the table and flag --- Makefile | 3 ++- README.md | 4 ++++ docs/moving-pictures/table.qza | Bin 41773 -> 53704 bytes 3 files changed, 6 insertions(+), 1 deletion(-) diff --git a/Makefile b/Makefile index c56b49fa3..edba7b44c 100644 --- a/Makefile +++ b/Makefile @@ -54,4 +54,5 @@ docs: --i-pcoa docs/moving-pictures/unweighted_unifrac_pcoa_results.qza \ --m-sample-metadata-file docs/moving-pictures/sample_metadata.tsv \ --m-feature-metadata-file docs/moving-pictures/taxonomy.qza \ - --o-visualization docs/moving-pictures/empress-tree-tandem.qzv + --o-visualization docs/moving-pictures/empress-tree-tandem.qzv \ + --p-filter-extra-samples diff --git a/README.md b/README.md index 8779286f8..74d83ab72 100644 --- a/README.md +++ b/README.md @@ -78,6 +78,9 @@ of the samples in the ordination (if the ordination was made using a *filtered table*). If you'd like to read more about this, there's some informal discussion in [pull request 237](https://github.com/biocore/empress/pull/237). +The command below uses the *raw dataset* and removes extra samples not +represented in the ordination (using the `--p-filter-extra-samples` flag): + ```bash qiime empress plot \ --i-tree docs/moving-pictures/rooted-tree.qza \ @@ -86,6 +89,7 @@ qiime empress plot \ --m-sample-metadata-file docs/moving-pictures/sample_metadata.tsv \ --m-feature-metadata-file docs/moving-pictures/taxonomy.qza \ --o-visualization docs/moving-pictures/empress-tree-tandem.qzv + --p-filter-extra-samples ``` This generates a visualization of a phylogenetic tree alongside a visualization diff --git a/docs/moving-pictures/table.qza b/docs/moving-pictures/table.qza index ae6b1cde7ff933d3f2e024628f9fd262fae8750b..c26b61643503afdefb3ccee2abdbf83644d057ca 100644 GIT binary patch literal 53704 zcmc$_V~}Ofzpq*BvTeI|+3d1y+qP|+UF@=L+qUiMvN`>`n0x;B&P1F!XXeg}j2$~- 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+- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 74d83ab72..91b3d4d71 100644 --- a/README.md +++ b/README.md @@ -88,7 +88,7 @@ qiime empress plot \ --i-feature-table docs/moving-pictures/table.qza \ --m-sample-metadata-file docs/moving-pictures/sample_metadata.tsv \ --m-feature-metadata-file docs/moving-pictures/taxonomy.qza \ - --o-visualization docs/moving-pictures/empress-tree-tandem.qzv + --o-visualization docs/moving-pictures/empress-tree-tandem.qzv \ --p-filter-extra-samples ```