Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

Sign up for GitHub

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Jump to bottom

HUMAnN3: single-end reads equal to 50 bp, resulting in zero species #62

Open
zhangshuyue1 opened this issue Feb 11, 2025 · 0 comments
Open

HUMAnN3: single-end reads equal to 50 bp, resulting in zero species #62

zhangshuyue1 opened this issue Feb 11, 2025 · 0 comments

Comments

@zhangshuyue1
Copy link

I’m encountering complete classification failure using MetaPhlAn4/HUMAnN3 with single-end 50bp metagenomic data. Despite proper database configuration and parameter tuning, all reads remain unclassified. Has anyone resolved similar issues?
For example:
@S250118066L1C001R00100001100
CTATGATTGGAAGCTTCTTGAGACCTCCCCAGAAGCAGATGCAAGTCGGA
+
FDECFADDFFCAFF@BFDDFCFCFF@FFFFCF@EFFEFA@FFCEFAFFFC
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Assignees
No one assigned
Labels
None yet
Projects
None yet
Milestone
No milestone
Development

No branches or pull requests
1 participant
@zhangshuyue1