diff --git a/packages/aws-cdk-lib/aws-autoscaling/lib/auto-scaling-group.ts b/packages/aws-cdk-lib/aws-autoscaling/lib/auto-scaling-group.ts
index e38c2456ebcb5..e0105d02ce4dd 100644
--- a/packages/aws-cdk-lib/aws-autoscaling/lib/auto-scaling-group.ts
+++ b/packages/aws-cdk-lib/aws-autoscaling/lib/auto-scaling-group.ts
@@ -1759,6 +1759,9 @@ export class AutoScalingGroup extends AutoScalingGroupBase implements
     if (props.blockDevices) {
       throw new Error('Setting \'blockDevices\' must not be set when \'launchTemplate\' or \'mixedInstancesPolicy\' is set');
     }
+    if (props.requireImdsv2) {
+      throw new Error('Setting \'requireImdsv2\' must not be set when \'launchTemplate\' or \'mixedInstancesPolicy\' is set');
+    }
   }
 
   /**
diff --git a/packages/aws-cdk-lib/aws-autoscaling/test/auto-scaling-group.test.ts b/packages/aws-cdk-lib/aws-autoscaling/test/auto-scaling-group.test.ts
index 1ccbdd5f423ba..2b78904dd300c 100644
--- a/packages/aws-cdk-lib/aws-autoscaling/test/auto-scaling-group.test.ts
+++ b/packages/aws-cdk-lib/aws-autoscaling/test/auto-scaling-group.test.ts
@@ -2947,6 +2947,26 @@ describe('InstanceMaintenancePolicy', () => {
       });
     }).toThrow(/The difference between minHealthyPercentage and maxHealthyPercentage cannot be greater than 100, got 200/);
   });
+
+  test('throws if requireImdsv2 set when launchTemplate is set', () => {
+    // GIVEN
+    const stack = new cdk.Stack();
+    stack.node.setContext(AUTOSCALING_GENERATE_LAUNCH_TEMPLATE, true);
+    const vpc = mockVpc(stack);
+    const lt = LaunchTemplate.fromLaunchTemplateAttributes(stack, 'imported-lt', {
+      launchTemplateId: 'test-lt-id',
+      versionNumber: '0',
+    });
+
+    // THEN
+    expect(() => {
+      new autoscaling.AutoScalingGroup(stack, 'MyFleet', {
+        vpc,
+        launchTemplate: lt,
+        requireImdsv2: true,
+      });
+    }).toThrow(/Setting \'requireImdsv2\' must not be set when \'launchTemplate\' or \'mixedInstancesPolicy\' is set/);
+  });
 });
 
 function mockSecurityGroup(stack: cdk.Stack) {