diff --git a/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.py b/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.py
index 15631d15f2acd..8b2811eb63467 100644
--- a/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.py
+++ b/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.py
@@ -36,3 +36,11 @@ def f8(x: bytes = b"50 character byte stringgggggggggggggggggggggggggg\xff") ->
 baz: bytes = b"50 character byte stringgggggggggggggggggggggggggg"
 
 qux: bytes = b"51 character byte stringggggggggggggggggggggggggggg\xff"
+
+
+class Demo:
+    """Docstrings are excluded from this rule. Some padding."""
+
+
+def func() -> None:
+    """Docstrings are excluded from this rule. Some padding."""
diff --git a/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.pyi b/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.pyi
index d2f55531a2660..71064d9bdbd02 100644
--- a/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.pyi
+++ b/crates/ruff/resources/test/fixtures/flake8_pyi/PYI053.pyi
@@ -28,3 +28,9 @@ bar: str = "51 character stringgggggggggggggggggggggggggggggggg"  # Error: PYI05
 baz: bytes = b"50 character byte stringgggggggggggggggggggggggggg"  # OK
 
 qux: bytes = b"51 character byte stringggggggggggggggggggggggggggg\xff"  # Error: PYI053
+
+class Demo:
+    """Docstrings are excluded from this rule. Some padding."""  # OK
+
+def func() -> None:
+    """Docstrings are excluded from this rule. Some padding."""  # OK
diff --git a/crates/ruff/src/rules/flake8_pyi/rules/string_or_bytes_too_long.rs b/crates/ruff/src/rules/flake8_pyi/rules/string_or_bytes_too_long.rs
index cecaed2685707..ff5ab572e88bd 100644
--- a/crates/ruff/src/rules/flake8_pyi/rules/string_or_bytes_too_long.rs
+++ b/crates/ruff/src/rules/flake8_pyi/rules/string_or_bytes_too_long.rs
@@ -2,6 +2,7 @@ use rustpython_parser::ast::{self, Constant, Expr, Ranged};
 
 use ruff_diagnostics::{AlwaysAutofixableViolation, Diagnostic, Edit, Fix};
 use ruff_macros::{derive_message_formats, violation};
+use ruff_python_ast::helpers::is_docstring_stmt;
 
 use crate::checkers::ast::Checker;
 use crate::registry::AsRule;
@@ -41,6 +42,11 @@ impl AlwaysAutofixableViolation for StringOrBytesTooLong {
 
 /// PYI053
 pub(crate) fn string_or_bytes_too_long(checker: &mut Checker, expr: &Expr) {
+    // Ignore docstrings.
+    if is_docstring_stmt(checker.semantic().stmt()) {
+        return;
+    }
+
     let length = match expr {
         Expr::Constant(ast::ExprConstant {
             value: Constant::Str(s),
diff --git a/crates/ruff/src/rules/flake8_pyi/snapshots/ruff__rules__flake8_pyi__tests__PYI053_PYI053.pyi.snap b/crates/ruff/src/rules/flake8_pyi/snapshots/ruff__rules__flake8_pyi__tests__PYI053_PYI053.pyi.snap
index 275d11d8b435d..951ca3acb183b 100644
--- a/crates/ruff/src/rules/flake8_pyi/snapshots/ruff__rules__flake8_pyi__tests__PYI053_PYI053.pyi.snap
+++ b/crates/ruff/src/rules/flake8_pyi/snapshots/ruff__rules__flake8_pyi__tests__PYI053_PYI053.pyi.snap
@@ -89,6 +89,8 @@ PYI053.pyi:30:14: PYI053 [*] String and bytes literals longer than 50 characters
 29 | 
 30 | qux: bytes = b"51 character byte stringggggggggggggggggggggggggggg\xff"  # Error: PYI053
    |              ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PYI053
+31 | 
+32 | class Demo:
    |
    = help: Replace with `...`
 
@@ -98,5 +100,8 @@ PYI053.pyi:30:14: PYI053 [*] String and bytes literals longer than 50 characters
 29 29 | 
 30    |-qux: bytes = b"51 character byte stringggggggggggggggggggggggggggg\xff"  # Error: PYI053
    30 |+qux: bytes = ...  # Error: PYI053
+31 31 | 
+32 32 | class Demo:
+33 33 |     """Docstrings are excluded from this rule. Some padding."""  # OK