diff --git a/crates/ruff/resources/test/fixtures/pandas_vet/PD101.py b/crates/ruff/resources/test/fixtures/pandas_vet/PD101.py
new file mode 100644
index 0000000000000..157c5e47a956a
--- /dev/null
+++ b/crates/ruff/resources/test/fixtures/pandas_vet/PD101.py
@@ -0,0 +1,27 @@
+import pandas as pd
+
+
+data = pd.Series(range(1000))
+
+# PD101
+data.nunique() <= 1
+data.nunique(dropna=True) <= 1
+data.nunique(dropna=False) <= 1
+data.nunique() == 1
+data.nunique(dropna=True) == 1
+data.nunique(dropna=False) == 1
+data.nunique() != 1
+data.nunique(dropna=True) != 1
+data.nunique(dropna=False) != 1
+data.nunique() > 1
+data.dropna().nunique() == 1
+data[data.notnull()].nunique() == 1
+
+# No violation of this rule
+data.nunique() == 0  # empty
+data.nunique() >= 1  # not-empty
+data.nunique() < 1  # empty
+data.nunique() == 2  # not constant
+data.unique() == 1  # not `nunique`
+
+{"hello": "world"}.nunique() == 1  # no pd.Series
diff --git a/crates/ruff/src/checkers/ast/mod.rs b/crates/ruff/src/checkers/ast/mod.rs
index 3c6e1b2b9e0d0..a168fcd2483bf 100644
--- a/crates/ruff/src/checkers/ast/mod.rs
+++ b/crates/ruff/src/checkers/ast/mod.rs
@@ -3285,6 +3285,15 @@ where
                 if self.enabled(Rule::YodaConditions) {
                     flake8_simplify::rules::yoda_conditions(self, expr, left, ops, comparators);
                 }
+                if self.enabled(Rule::PandasNuniqueConstantSeriesCheck) {
+                    pandas_vet::rules::nunique_constant_series_check(
+                        self,
+                        expr,
+                        left,
+                        ops,
+                        comparators,
+                    );
+                }
             }
             Expr::Constant(ast::ExprConstant {
                 value: Constant::Int(_) | Constant::Float(_) | Constant::Complex { .. },
diff --git a/crates/ruff/src/codes.rs b/crates/ruff/src/codes.rs
index 3a2d13a41e1d6..b77dccb198269 100644
--- a/crates/ruff/src/codes.rs
+++ b/crates/ruff/src/codes.rs
@@ -604,6 +604,7 @@ pub fn code_to_rule(linter: Linter, code: &str) -> Option<(RuleGroup, Rule)> {
         (PandasVet, "012") => (RuleGroup::Unspecified, rules::pandas_vet::rules::PandasUseOfDotReadTable),
         (PandasVet, "013") => (RuleGroup::Unspecified, rules::pandas_vet::rules::PandasUseOfDotStack),
         (PandasVet, "015") => (RuleGroup::Unspecified, rules::pandas_vet::rules::PandasUseOfPdMerge),
+        (PandasVet, "101") => (RuleGroup::Unspecified, rules::pandas_vet::rules::PandasNuniqueConstantSeriesCheck),
         (PandasVet, "901") => (RuleGroup::Unspecified, rules::pandas_vet::rules::PandasDfVariableName),
 
         // flake8-errmsg
diff --git a/crates/ruff/src/rules/pandas_vet/mod.rs b/crates/ruff/src/rules/pandas_vet/mod.rs
index 6319a0f5cbd05..b57f0836dfe6f 100644
--- a/crates/ruff/src/rules/pandas_vet/mod.rs
+++ b/crates/ruff/src/rules/pandas_vet/mod.rs
@@ -344,6 +344,7 @@ mod tests {
         Path::new("pandas_use_of_dot_read_table.py")
     )]
     #[test_case(Rule::PandasUseOfInplaceArgument, Path::new("PD002.py"))]
+    #[test_case(Rule::PandasNuniqueConstantSeriesCheck, Path::new("PD101.py"))]
     fn paths(rule_code: Rule, path: &Path) -> Result<()> {
         let snapshot = format!("{}_{}", rule_code.noqa_code(), path.to_string_lossy());
         let diagnostics = test_path(
diff --git a/crates/ruff/src/rules/pandas_vet/rules/mod.rs b/crates/ruff/src/rules/pandas_vet/rules/mod.rs
index 0a52e1878324a..7a137babb3bb5 100644
--- a/crates/ruff/src/rules/pandas_vet/rules/mod.rs
+++ b/crates/ruff/src/rules/pandas_vet/rules/mod.rs
@@ -2,6 +2,7 @@ pub(crate) use assignment_to_df::*;
 pub(crate) use attr::*;
 pub(crate) use call::*;
 pub(crate) use inplace_argument::*;
+pub(crate) use nunique_constant_series_check::*;
 pub(crate) use pd_merge::*;
 pub(crate) use read_table::*;
 pub(crate) use subscript::*;
@@ -10,6 +11,7 @@ pub(crate) mod assignment_to_df;
 pub(crate) mod attr;
 pub(crate) mod call;
 pub(crate) mod inplace_argument;
+pub(crate) mod nunique_constant_series_check;
 pub(crate) mod pd_merge;
 pub(crate) mod read_table;
 pub(crate) mod subscript;
diff --git a/crates/ruff/src/rules/pandas_vet/rules/nunique_constant_series_check.rs b/crates/ruff/src/rules/pandas_vet/rules/nunique_constant_series_check.rs
new file mode 100644
index 0000000000000..9edffcf25ee8c
--- /dev/null
+++ b/crates/ruff/src/rules/pandas_vet/rules/nunique_constant_series_check.rs
@@ -0,0 +1,122 @@
+use num_traits::One;
+use rustpython_parser::ast::{self, CmpOp, Constant, Expr, Ranged};
+
+use ruff_diagnostics::Diagnostic;
+use ruff_diagnostics::Violation;
+use ruff_macros::{derive_message_formats, violation};
+
+use crate::checkers::ast::Checker;
+use crate::rules::pandas_vet::helpers::{test_expression, Resolution};
+
+/// ## What it does
+/// Check for uses of `.nunique()` to check if a Pandas Series is constant
+/// (i.e., contains only one unique value).
+///
+/// ## Why is this bad?
+/// `.nunique()` is computationally inefficient for checking if a Series is
+/// constant.
+///
+/// Consider, for example, a Series of length `n` that consists of increasing
+/// integer values (e.g., 1, 2, 3, 4). The `.nunique()` method will iterate
+/// over the entire Series to count the number of unique values. But in this
+/// case, we can detect that the Series is non-constant after visiting the
+/// first two values, which are non-equal.
+///
+/// In general, `.nunique()` requires iterating over the entire Series, while a
+/// more efficient approach allows short-circuiting the operation as soon as a
+/// non-equal value is found.
+///
+/// Instead of calling `.nunique()`, convert the Series to a NumPy array, and
+/// check if all values in the array are equal to the first observed value.
+///
+/// ## Example
+/// ```python
+/// import pandas as pd
+///
+/// data = pd.Series(range(1000))
+/// if data.nunique() <= 1:
+///     print("Series is constant")
+/// ```
+///
+/// Use instead:
+/// ```python
+/// import pandas as pd
+///
+/// data = pd.Series(range(1000))
+/// array = data.to_numpy()
+/// if array.shape[0] == 0 or (array[0] == array).all():
+///     print("Series is constant")
+/// ```
+///
+/// ## References
+/// - [Pandas Cookbook: "Constant Series"](https://pandas.pydata.org/docs/user_guide/cookbook.html#constant-series)
+/// - [Pandas documentation: `nunique`](https://pandas.pydata.org/docs/reference/api/pandas.Series.nunique.html)
+#[violation]
+pub struct PandasNuniqueConstantSeriesCheck;
+
+impl Violation for PandasNuniqueConstantSeriesCheck {
+    #[derive_message_formats]
+    fn message(&self) -> String {
+        format!("Using `series.nunique()` for checking that a series is constant is inefficient")
+    }
+}
+
+/// PD101
+pub(crate) fn nunique_constant_series_check(
+    checker: &mut Checker,
+    expr: &Expr,
+    left: &Expr,
+    ops: &[CmpOp],
+    comparators: &[Expr],
+) {
+    let ([op], [right]) = (ops, comparators) else {
+        return;
+    };
+
+    // Operators may be ==, !=, <=, >.
+    if !matches!(op, CmpOp::Eq | CmpOp::NotEq | CmpOp::LtE | CmpOp::Gt,) {
+        return;
+    }
+
+    // Right should be the integer 1.
+    if !is_constant_one(right) {
+        return;
+    }
+
+    // Check if call is `.nuniuqe()`.
+    let Expr::Call(ast::ExprCall { func, .. }) = left else {
+        return;
+    };
+
+    let Expr::Attribute(ast::ExprAttribute { value, attr, .. }) = func.as_ref() else {
+        return;
+    };
+
+    if attr.as_str() != "nunique" {
+        return;
+    }
+
+    // Avoid flagging on non-Series (e.g., `{"a": 1}.at[0]`).
+    if !matches!(
+        test_expression(value, checker.semantic()),
+        Resolution::RelevantLocal
+    ) {
+        return;
+    }
+
+    checker.diagnostics.push(Diagnostic::new(
+        PandasNuniqueConstantSeriesCheck,
+        expr.range(),
+    ));
+}
+
+/// Return `true` if an [`Expr`] is a constant `1`.
+fn is_constant_one(expr: &Expr) -> bool {
+    match expr {
+        Expr::Constant(constant) => match &constant.value {
+            Constant::Int(int) => int.is_one(),
+            _ => false,
+        },
+        _ => false,
+    }
+}
diff --git a/crates/ruff/src/rules/pandas_vet/snapshots/ruff__rules__pandas_vet__tests__PD101_PD101.py.snap b/crates/ruff/src/rules/pandas_vet/snapshots/ruff__rules__pandas_vet__tests__PD101_PD101.py.snap
new file mode 100644
index 0000000000000..ddfdd060ba947
--- /dev/null
+++ b/crates/ruff/src/rules/pandas_vet/snapshots/ruff__rules__pandas_vet__tests__PD101_PD101.py.snap
@@ -0,0 +1,122 @@
+---
+source: crates/ruff/src/rules/pandas_vet/mod.rs
+---
+PD101.py:7:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+  |
+6 | # PD101
+7 | data.nunique() <= 1
+  | ^^^^^^^^^^^^^^^^^^^ PD101
+8 | data.nunique(dropna=True) <= 1
+9 | data.nunique(dropna=False) <= 1
+  |
+
+PD101.py:8:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+ 6 | # PD101
+ 7 | data.nunique() <= 1
+ 8 | data.nunique(dropna=True) <= 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+ 9 | data.nunique(dropna=False) <= 1
+10 | data.nunique() == 1
+   |
+
+PD101.py:9:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+ 7 | data.nunique() <= 1
+ 8 | data.nunique(dropna=True) <= 1
+ 9 | data.nunique(dropna=False) <= 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+10 | data.nunique() == 1
+11 | data.nunique(dropna=True) == 1
+   |
+
+PD101.py:10:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+ 8 | data.nunique(dropna=True) <= 1
+ 9 | data.nunique(dropna=False) <= 1
+10 | data.nunique() == 1
+   | ^^^^^^^^^^^^^^^^^^^ PD101
+11 | data.nunique(dropna=True) == 1
+12 | data.nunique(dropna=False) == 1
+   |
+
+PD101.py:11:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+ 9 | data.nunique(dropna=False) <= 1
+10 | data.nunique() == 1
+11 | data.nunique(dropna=True) == 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+12 | data.nunique(dropna=False) == 1
+13 | data.nunique() != 1
+   |
+
+PD101.py:12:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+10 | data.nunique() == 1
+11 | data.nunique(dropna=True) == 1
+12 | data.nunique(dropna=False) == 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+13 | data.nunique() != 1
+14 | data.nunique(dropna=True) != 1
+   |
+
+PD101.py:13:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+11 | data.nunique(dropna=True) == 1
+12 | data.nunique(dropna=False) == 1
+13 | data.nunique() != 1
+   | ^^^^^^^^^^^^^^^^^^^ PD101
+14 | data.nunique(dropna=True) != 1
+15 | data.nunique(dropna=False) != 1
+   |
+
+PD101.py:14:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+12 | data.nunique(dropna=False) == 1
+13 | data.nunique() != 1
+14 | data.nunique(dropna=True) != 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+15 | data.nunique(dropna=False) != 1
+16 | data.nunique() > 1
+   |
+
+PD101.py:15:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+13 | data.nunique() != 1
+14 | data.nunique(dropna=True) != 1
+15 | data.nunique(dropna=False) != 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+16 | data.nunique() > 1
+17 | data.dropna().nunique() == 1
+   |
+
+PD101.py:16:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+14 | data.nunique(dropna=True) != 1
+15 | data.nunique(dropna=False) != 1
+16 | data.nunique() > 1
+   | ^^^^^^^^^^^^^^^^^^ PD101
+17 | data.dropna().nunique() == 1
+18 | data[data.notnull()].nunique() == 1
+   |
+
+PD101.py:17:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+15 | data.nunique(dropna=False) != 1
+16 | data.nunique() > 1
+17 | data.dropna().nunique() == 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+18 | data[data.notnull()].nunique() == 1
+   |
+
+PD101.py:18:1: PD101 Using `series.nunique()` for checking that a series is constant is inefficient
+   |
+16 | data.nunique() > 1
+17 | data.dropna().nunique() == 1
+18 | data[data.notnull()].nunique() == 1
+   | ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ PD101
+19 | 
+20 | # No violation of this rule
+   |
+
+
diff --git a/ruff.schema.json b/ruff.schema.json
index 353136f6944ff..8732c73890891 100644
--- a/ruff.schema.json
+++ b/ruff.schema.json
@@ -2090,6 +2090,9 @@
         "PD012",
         "PD013",
         "PD015",
+        "PD1",
+        "PD10",
+        "PD101",
         "PD9",
         "PD90",
         "PD901",