diff --git a/sdks/python/tox.ini b/sdks/python/tox.ini
index 8beadfcf13b8..1e797d96074f 100644
--- a/sdks/python/tox.ini
+++ b/sdks/python/tox.ini
@@ -17,7 +17,7 @@
 
 [tox]
 # new environments will be excluded by default unless explicitly added to envlist.
-envlist = py38,py39,py310,py311,py38-{cloud,cython,docs,lint,mypy,cloudcoverage,dask},py39-{cloud,cython},py310-{cloud,cython,dask},py311-{cloud,cython,dask},whitespacelint
+envlist = py38,py39,py310,py311,py38-{cloud,docs,lint,mypy,cloudcoverage,dask},py39-{cloud},py310-{cloud,dask},py311-{cloud,dask},whitespacelint
 toxworkdir = {toxinidir}/target/{env:ENV_NAME:.tox}
 
 [pycodestyle]
@@ -44,8 +44,6 @@ allowlist_externals =
   curl
   ./codecov
   chmod
-deps =
-  cython: cython==0.29.33
 setenv =
   RUN_SKIPPED_PY3_TESTS=0
   # Use an isolated tmp dir for tests that get slowed down by scanning /tmp.
@@ -66,6 +64,7 @@ commands_pre =
   bash {toxinidir}/scripts/run_tox_cleanup.sh
 commands_post =
   bash {toxinidir}/scripts/run_tox_cleanup.sh
+
 commands = false {envname} is misconfigured
 
 [testenv:py{38,39,310,311}]
@@ -80,28 +79,18 @@ commands =
 install_command = {envbindir}/python.exe {envbindir}/pip.exe install --retries 10 {opts} {packages}
 list_dependencies_command = {envbindir}/python.exe {envbindir}/pip.exe freeze
 
-[testenv:py{38,39,310,311}-cython]
-# cython tests are only expected to work in linux (2.x and 3.x)
-# If we want to add other platforms in the future, it should be:
-# `platform = linux2|darwin|...`
-# See https://docs.python.org/2/library/sys.html#sys.platform for platform codes
-platform = linux
-commands =
-  # TODO(https://github.com/apache/beam/issues/20051): Remove this build_ext invocation once local source no longer
-  #   shadows the installed apache_beam.
-  python setup.py build_ext --inplace
-  python apache_beam/examples/complete/autocomplete_test.py
-  bash {toxinidir}/scripts/run_pytest.sh {envname} "{posargs}"
-
 [testenv:py{38,39,310,311}-cloud]
+; extras = test,gcp,interactive,dataframe,aws,azure
 extras = test,gcp,interactive,dataframe,aws,azure
 commands =
+  python apache_beam/examples/complete/autocomplete_test.py
   bash {toxinidir}/scripts/run_pytest.sh {envname} "{posargs}"
 
 [testenv:py{38,39,310,311}-dask]
 extras = test,dask
 commands =
   bash {toxinidir}/scripts/run_pytest.sh {envname} "{posargs}"
+
 [testenv:py38-cloudcoverage]
 deps =
   pytest-cov==3.0.0
@@ -123,7 +112,6 @@ commands =
 setenv =
 # keep the version of pylint in sync with the 'rev' in .pre-commit-config.yaml
 deps =
-  -r build-requirements.txt
   astroid<2.17.0,>=2.15.6
   pycodestyle==2.8.0
   pylint==2.17.5
@@ -142,7 +130,6 @@ commands =
 
 [testenv:py38-mypy]
 deps =
-  -r build-requirements.txt
   mypy==0.790
   dask==2022.01.0
   distributed==2022.01.0
@@ -172,7 +159,6 @@ commands =
 # Used by hdfs_integration_test.sh. Do not run this directly, as it depends on
 # nodes defined in hdfs_integration_test/docker-compose.yml.
 deps =
-  -r build-requirements.txt
   holdup==1.8.0
 extras =
   gcp
@@ -205,7 +191,6 @@ commands_pre =
 # Do not run this directly, as it depends on nodes defined in
 # azure/integration_test/docker-compose.yml.
 deps =
-  -r build-requirements.txt
 extras =
   azure
 passenv = REQUESTS_CA_BUNDLE
@@ -334,7 +319,6 @@ commands =
 
 [testenv:py{38,39,310,311}-pytorch-{19,110,111,112,113}]
 deps =
-  -r build-requirements.txt
   19: torch>=1.9.0,<1.10.0
   110: torch>=1.10.0,<1.11.0
   111: torch>=1.11.0,<1.12.0
@@ -352,7 +336,6 @@ commands =
 
 [testenv:py{38,39,310}-pytorch-200]
 deps =
-  -r build-requirements.txt
   200: torch>=2.0.0,<2.1.0
 extras = test,gcp
 # Don't set TMPDIR to avoid "AF_UNIX path too long" errors in certain tests.
@@ -386,7 +369,6 @@ commands =
 
 [testenv:py{38,39,310}-tensorflow-212]
 deps =
-  -r build-requirements.txt
   212: tensorflow>=2.12rc1,<2.13
 extras = test,gcp
 commands =
@@ -398,7 +380,6 @@ commands =
 
 [testenv:py{38,39,310}-xgboost-{160,170}]
 deps =
-  -r build-requirements.txt
   160:
     xgboost>=1.6.0,<1.7.0
     datatable==1.0.0
@@ -415,7 +396,6 @@ commands =
 
 [testenv:py{38,39,310,311}-transformers-{428,429,430}]
 deps =
-  -r build-requirements.txt
   428: transformers>=4.28.0,<4.29.0
   429: transformers>=4.29.0,<4.30.0
   430: transformers>=4.30.0,<4.31.0
@@ -433,7 +413,6 @@ commands =
 
 [testenv:py{38,311}-vertex-ai]
 deps =
-  -r build-requirements.txt
   tensorflow==2.12.0
 extras = test,gcp
 commands =