You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi, I'm trying to do an rpsblast using ncbi-blast-2.5.0+. I have a file containing gi numbers as my query and my command line is below.
rpsblast -query t -db results/Cog/Cog -out rps-blast.out -evalue 1e-2 -outfmt 6
Oddly the rpbslast keeps giving me this error:
Warning: [rpsblast] Error initializing remote BLAST database data loader: Protein BLAST database 'Cog/Cog nr' does not exist in the NCBI servers
My query file (t) looks like this. I've tried to remove search using just the numbers (292833481) but it still doesn't solve the problem.
gi|292833481
gi|383341230
gi|289693981
However, when I try to the same search but using a fasta file as query, it runs fine and gives the results I need. rpsblast -query GCF_000005845.2_ASM584v2_protein.faa -db results/Cog/Cog -out rps-blast.out -evalue 1e-2 -outfmt 6
Is there something that I did wrong here? What is the correct way to format query a list of gi's for blast? Thanks!
The text was updated successfully, but these errors were encountered:
Hi, I'm trying to do an rpsblast using ncbi-blast-2.5.0+. I have a file containing gi numbers as my query and my command line is below.
rpsblast -query t -db results/Cog/Cog -out rps-blast.out -evalue 1e-2 -outfmt 6
Oddly the rpbslast keeps giving me this error:
Warning: [rpsblast] Error initializing remote BLAST database data loader: Protein BLAST database 'Cog/Cog nr' does not exist in the NCBI servers
My query file (t) looks like this. I've tried to remove search using just the numbers (292833481) but it still doesn't solve the problem.
gi|292833481
gi|383341230
gi|289693981
However, when I try to the same search but using a fasta file as query, it runs fine and gives the results I need.
rpsblast -query GCF_000005845.2_ASM584v2_protein.faa -db results/Cog/Cog -out rps-blast.out -evalue 1e-2 -outfmt 6
Is there something that I did wrong here? What is the correct way to format query a list of gi's for blast? Thanks!
The text was updated successfully, but these errors were encountered: