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Inconsistant transfer #146

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gdroc opened this issue Mar 16, 2023 · 0 comments
Open

Inconsistant transfer #146

gdroc opened this issue Mar 16, 2023 · 0 comments

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@gdroc
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gdroc commented Mar 16, 2023

Hi,

I want to compare 4 genomes, and I use liftoff to transfer annotations from one genome to another. In the vast majority of cases, it works very fine, but in the example in attachment, I have the impression that it does not keep the expected gene.
In the JBrowse, the track "C. micrantha" matches the original annotations, and we found CITMI_009t013070 (expected to be on chromosome 9).
In the track "C. micrantha remove CITMI_009t013070", I simply deleted this gene from the original GFF3, and I find the expected gene CITMI_001t000040, which also seems to have a much better coverage than CITMI_009t013070. When I look at the output of minimap2, I find the same alignments.
I tried to play with several liftoff parameters without success (-polish, -s)
Do you have any idea where this could be coming from?
Regards
Gaetan
Capture d’écran 2023-03-16 à 14 30 12

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