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show user the result of threshold value and give option to change the value. Repeat until user is satisfied
fit histogram to a function (maybe a double gaussian) to indentify the background and nucleus peaks
start at high threshold values and measure size of the obtained nucleus. Slowly increase until the difference on its area is really big (alternative, make big jump, and cut the diference into half, until finding the best value)
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Different possible approaches to this:
The text was updated successfully, but these errors were encountered: