diff --git a/packages/components/CHANGELOG.md b/packages/components/CHANGELOG.md
index ed33677b55a82a..d7a838344f5cd5 100644
--- a/packages/components/CHANGELOG.md
+++ b/packages/components/CHANGELOG.md
@@ -18,6 +18,10 @@
 -   `ToolsPanel`: fix a performance issue ([#56770](https://github.com/WordPress/gutenberg/pull/56770)).
 -   `BorderControl`: adjust `BorderControlDropdown` Button size to fix misaligned border ([#56730](https://github.com/WordPress/gutenberg/pull/56730)).
 
+### Internal
+
+-   `DropdownMenuV2Ariakit`: prevent prefix collapsing if all radios or checkboxes are unselected ([#56720](https://github.com/WordPress/gutenberg/pull/56720)).
+
 ## 25.13.0 (2023-11-29)
 
 ### Enhancements
diff --git a/packages/components/src/dropdown-menu-v2-ariakit/styles.ts b/packages/components/src/dropdown-menu-v2-ariakit/styles.ts
index 465bdb1aebb30e..eaa249ae86b78c 100644
--- a/packages/components/src/dropdown-menu-v2-ariakit/styles.ts
+++ b/packages/components/src/dropdown-menu-v2-ariakit/styles.ts
@@ -212,6 +212,18 @@ export const ItemPrefixWrapper = styled.span`
 	/* Always occupy the first column, even when auto-collapsing */
 	grid-column: 1;
 
+	/*
+	 * Even when the item is not checked, occupy the same screen space to avoid
+	 * the space collapside when no items are checked.
+	 */
+	${ DropdownMenuCheckboxItem } > &,
+	${ DropdownMenuRadioItem } > & {
+		/* Same width as the check icons */
+		min-width: ${ space( 6 ) };
+	}
+
+	${ DropdownMenuCheckboxItem } > &,
+	${ DropdownMenuRadioItem } > &,
 	&:not( :empty ) {
 		margin-inline-end: ${ space( 2 ) };
 	}