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run_daytona.sh and Daytona-Combine

This wrapper is used to tidy and analyse Clear Labs data using a custom cleaner and the Daytona-combine pipeline.

Prerequisites

  1. Install Nextflow (>=21.10.3)

  2. Install Python3.7+ and BioPython

May need to use update-alternatives to set correct version of python, ie:

$ sudo update-alternatives --install /usr/bin/python3 python3 /home/${USER}/miniconda3/bin/python3.8 4

BioPython:

$ python3 -m pip install biopython
  1. Install Bash Needs (may need to install individually)
$ sudo apt-get update && sudo apt-get install -y \
    build-essential \
    libssl-dev \
    uuid-dev \
    libgpgme11-dev \
    squashfs-tools \
    libseccomp-dev \
    pkg-config

$ sudo apt-get install libtool-bin
  1. Install Go
$ export VERSION=1.11 OS=linux ARCH=amd64 && wget https://dl.google.com/go/go1.22.2.linux-amd64.tar.gz && sudo tar -C /usr/local -xzvf go1.22.2.linux-amd64.tar.gz && rm go1.22.2.linux-amd64.tar.gz

$ echo 'export GOPATH=${HOME}/go' >> ~/.bashrc && \
    echo 'export PATH=/usr/local/go/bin:${PATH}:${GOPATH}/bin' >> ~/.bashrc && \
    source ~/.bashrc
  1. Install Singularity
$ wget https://github.com/sylabs/singularity/releases/download/v4.1.2/singularity-ce-4.1.2.tar.gz
$ tar -xzf singularity-ce-4.1.2.tar.gz
$ cd ./singularity-ce-4.1.2
$ ./mconfig
$ make -C ./builddir
$ sudo make -C ./builddir install
  1. Install the Code
$ git clone https://github.com/VADGS/Daytona.git

Set to path in bashrc:

export PATH="/home/${USER}/Applications/daytona:$PATH"

How to run

1) If the data is "raw" from Clear Labs:

  1. Download the tar file from Clear Labs
  2. Move the tar file into the desired directory
  3. Use the following command to invoke the pipeline:
$ run_daytona.sh -t tar

2) If the data has been previously downloaded and extracted/analzed from Clear Labs:

  1. Use the following command to invoke the pipeline:
$ run_daytona.sh -r path/to/directory -o desired/output/name

Results

All results can be found in the directory /output.