diff --git a/functions/api/routes/room/roomTimelineGET.js b/functions/api/routes/room/roomTimelineGET.js
index ffc6bf8..73bef05 100644
--- a/functions/api/routes/room/roomTimelineGET.js
+++ b/functions/api/routes/room/roomTimelineGET.js
@@ -69,6 +69,8 @@ module.exports = async (req, res) => {
       return timeline;
     });
 
+    await lifeTimelineDB.readLifeTimeline(client, roomId, user.userId);
+
     return res.status(statusCode.OK).send(
       util.success(statusCode.OK, responseMessage.GET_LIFE_TIMELINE_SUCCESS, {
         timelines,
diff --git a/functions/constants/responseMessage.js b/functions/constants/responseMessage.js
index 5171ff1..5ab35e7 100644
--- a/functions/constants/responseMessage.js
+++ b/functions/constants/responseMessage.js
@@ -69,6 +69,7 @@ module.exports = {
 
   // Life Timeline
   GET_LIFE_TIMELINE_SUCCESS: '생명 타임라인 조회 성공',
+  LIFE_TIMELINE_READ_SUCCESS: '생명 타임라인 읽음처리 완료',
 
   // Feed
   GET_FEED_SUCCES: '피드 조회 성공',
diff --git a/functions/db/lifeTimeline.js b/functions/db/lifeTimeline.js
index 74f2072..39633ee 100644
--- a/functions/db/lifeTimeline.js
+++ b/functions/db/lifeTimeline.js
@@ -28,7 +28,22 @@ const getLifeTimeline = async (client, roomId, userId) => {
   return convertSnakeToCamel.keysToCamel(rows);
 };
 
+const readLifeTimeline = async (client, roomId, userId) => {
+  const { rows } = await client.query(
+    `
+    UPDATE spark.life_timeline
+    SET is_read = TRUE
+    WHERE room_id = $1
+    AND receiver_id = $2
+    RETURNING *
+    `,
+    [roomId, userId],
+  );
+  return convertSnakeToCamel.keysToCamel(rows);
+};
+
 module.exports = {
   addLifeTimeline,
   getLifeTimeline,
+  readLifeTimeline,
 };