diff --git a/README.md b/README.md
index 1b35d4cec..52fb46758 100644
--- a/README.md
+++ b/README.md
@@ -166,7 +166,7 @@ To learn more about the docker pull rate limits and the open source software pro
 | [geNomad](https://hub.docker.com/r/staphb/genomad) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/genomad)](https://hub.docker.com/r/staphb/genomad) | <ul><li>[1.7.4](./genomad/1.7.4/)</li></ul> | https://github.com/apcamargo/genomad |
 | [GenoVi](https://hub.docker.com/r/staphb/genovi) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/genovi)](https://hub.docker.com/r/staphb/genovi) | <ul><li>[0.2.16](./genovi/0.2.16/)</li></ul> | https://github.com/robotoD/GenoVi |
 | [gfastats](https://hub.docker.com/r/staphb/gfastats) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/gfastats)](https://hub.docker.com/r/staphb/gfastats) | <ul><li>1.3.6</li></ul> | https://github.com/vgl-hub/gfastats |
-| [Gubbins](https://hub.docker.com/r/staphb/gubbins) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/gubbins)](https://hub.docker.com/r/staphb/gubbins) | <ul><li>[3.3.3](./gubbins/3.3.3/)</li></ul> | https://github.com/nickjcroucher/gubbins |
+| [Gubbins](https://hub.docker.com/r/staphb/gubbins) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/gubbins)](https://hub.docker.com/r/staphb/gubbins) | <ul><li>[3.3.3](./gubbins/3.3.3/)</li><li>[3.3.4](./gubbins/3.3.4/)</li></ul> | https://github.com/nickjcroucher/gubbins |
 | [heatcluster](https://hub.docker.com/r/staphb/heatcluster) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/heatcluster)](https://hub.docker.com/r/staphb/heatcluster) | <ul><li>[1.0.2c](./heatcluster/1.0.2c/)</li></ul> | https://github.com/DrB-S/heatcluster/tree/main |
 | [hmmer](https://hub.docker.com/r/staphb/hmmer) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/hmmer)](https://hub.docker.com/r/staphb/hmmer) | <ul><li>[3.3](hmmer/3.3/)</li><li>[3.3.2](hmmer/3.3.2/)</li><li>[3.4](./hmmer/3.4/)</li></ul> | http://hmmer.org/ |
 | [homopolish](https://hub.docker.com/r/staphb/homopolish) <br/> [![docker pulls](https://badgen.net/docker/pulls/staphb/homopolish)](https://hub.docker.com/r/staphb/homopolish) | <ul><li>0.4.1</li></ul> | https://github.com/ythuang0522/homopolish/ |
diff --git a/gubbins/3.3.4/Dockerfile b/gubbins/3.3.4/Dockerfile
new file mode 100644
index 000000000..abe8115b8
--- /dev/null
+++ b/gubbins/3.3.4/Dockerfile
@@ -0,0 +1,42 @@
+FROM mambaorg/micromamba:1.5.8 as app
+
+ARG GUBBINS_VER="3.3.4"
+
+USER root
+
+WORKDIR /
+
+LABEL base.image="mambaorg/micromamba:1.5.8"
+LABEL dockerfile.version="1"
+LABEL software="Gubbins"
+LABEL software.version="${GUBBINS_VER}"
+LABEL description="Genealogies Unbiased By recomBinations In Nucleotide Sequences"
+LABEL website="https://github.com/nickjcroucher/gubbins"
+LABEL license="https://github.com/nickjcroucher/gubbins/blob/master/LICENSE"
+LABEL maintainer="Kutluhan Incekara"
+LABEL maintainer.email="kutluhan.incekara@ct.gov"
+
+RUN apt-get update && apt-get install --no-install-recommends -y \
+    procps &&\
+    apt-get autoclean && rm -rf /var/lib/apt/lists/*
+
+RUN micromamba install --name base -c conda-forge -c bioconda gubbins=${GUBBINS_VER} && \
+    micromamba clean -a -y && \
+    mkdir /data
+
+ENV PATH="/opt/conda/bin/:${PATH}" \
+    LC_ALL=C.UTF-8
+
+CMD ["run_gubbins.py", "-h"]
+
+WORKDIR /data
+
+FROM app as test
+
+RUN run_gubbins.py -h
+
+WORKDIR /test
+
+RUN curl -LJO https://github.com/nickjcroucher/gubbins/raw/master/tests/data/alignment_file_multiple_lines_per_sequence.aln &&\
+    run_gubbins.py alignment_file_multiple_lines_per_sequence.aln
+
diff --git a/gubbins/3.3.4/README.md b/gubbins/3.3.4/README.md
new file mode 100644
index 000000000..d34c154d8
--- /dev/null
+++ b/gubbins/3.3.4/README.md
@@ -0,0 +1,26 @@
+# Gubbins container
+
+Main tool: [Gubbins](https://github.com/nickjcroucher/gubbins)
+  
+Code repository: https://github.com/nickjcroucher/gubbins
+
+Additional tools:
+- RAxML: 8.2.12
+- FastTree: 2.1.10
+- IQTree: 2.3.0
+- RAxML-NG: 1.0.1
+- Rapidnj: 2.3.2
+
+Basic information on how to use this tool:
+- executable: run_gubbins.py
+- help: -h
+- version: --version
+- description: Gubbins detects recombinations through the locally elevated densities of polymorphisms that arise when sequence segments are acquired from a donor genetically divergent from the set of analyzed sequences.
+
+Full documentation: https://github.com/nickjcroucher/gubbins/blob/master/docs/gubbins_manual.md
+
+## Example Usage
+
+```bash
+run_gubbins.py fasta_alignment.aln
+```